Abstract Contamination of water sources due to herbicide is of great concern. Clomazone is a pesticide with a high contamination potential which could possibility lixiviate to water streams. Changes caused by residual herbicide include flora modifications which are generally detrimental for some species. The lack of morphological studies performed in aquatic plants exposed to herbicide-contaminated environments has encouraged the development of our research. For the first time, we present a study that aimed to evaluate leaf injuries visible to the naked eye as well as microscopical effects which may be caused by clomazone on Pistia stratiotes. Pistia stratiotes was subjected to five concentrations of clomazone. Our analysis showed leaf injuries, especially after 15 days of clomazone application. Hormesis was observed when the water lettuce was subjected to the lower concentrations. Total leaf area showed increase following by reduction while injured until reaching the highest concentration. Although the concentrations of clomazone tested in our study are not lethal to water lettuce, such herbicide have still caused morphoanatomical damages on leaves which advocates for the use of P. stratiotes as a bioindicator of the presence of herbicides such as clomazone in water.

RESUMO O uso de acaricidas é o principal método de controle de Tetranychus ludeni (Acari: Tetranychidae) em cultivos hortícolas. Esse ácaro foi registrado causando danos em batata-doce (Ipomoea batatas (L.). A utilização de fungos patogênicos é uma alternativa ao controle químico. O objetivo foi avaliar a patogenicidade dos fungos Metarhizium anisopliae (Meetch) Sorok. e Beauveria bassiana (Bals.) Vuill. ao ácaro fitófago T. ludeni em batata-doce. Suspensões conidiais de M. anisopliae e B. bassiana, nas concentrações de 106 e 107 con.mL-1, foram aplicadas sobre folhas de batata-doce. Após 24 horas, cinco fêmeas recém-emergidas foram liberadas sobre as folhas. O bioensaio foi inteiramente randomizado, com arranjo fatorial 2 × 2 (duas espécies de fungos e duas concentrações) e controle (água destilada), com 10 replicações por tratamento. A avaliação consistiu na observação de três parâmetros biológicos do ácaro: mortalidade, oviposição e repelência, após 24, 48, 72 e 96 horas de contato com os fungos. Os isolados de M. anisopliae causam altas taxas de mortalidade de T. ludeni em laboratório. Beauveria bassiana tem potencial para suprimir futuras gerações do ácaro, reduzindo a taxa de oviposição. Comportamento de repelência nos ácaros não foi observado.

ABSTRACT The use of acaricides is the main control method for Tetranychus ludeni (Acari: Tetranychidae) in horticultural crops. This mite has been recorded causing damage to sweet potato (Ipomoea batatas L.). The use of pathogenic fungi is an alternative to chemical control. The objective was to evaluate the pathogenicity of the fungi Metarhizium anisopliae (Meetch) Sorok, and Beauveria bassiana (Bals.) Vuill. to phytophagous mite T. ludeni in sweet potato plants. Conidial suspensions of M. anisopliae and B. bassiana, at concentrations of 106 and 107 con.mL-1 were applied to sweet potato leaves. After 24 hours, five females of T. ludeni newly emerged were released on the leaves. The bioassay was arranged in a completely randomized design with factorial arrangement 2 × 2 (two species of fungi and two concentrations) plus the control (distilled water), with 10 repetitions per treatment. The evaluation consisted of observing of three biological parameters of the mite: mortality, oviposition, and repellency, after 24, 48, 72, and 96 hours of contact with the fungi. The isolates of M. anisopliae cause high mortality rates of T. ludeni in laboratory. Beauveria bassiana has the potential to suppress future generations of mite, reducing its oviposition rate. Repellency behavior was not observed.

Resumo: O objetivo deste trabalho foi avaliar os efeitos de diferentes arranjos de plantas de eucalipto sobre as características morfoanatômicas de lâminas foliares de plantas de capim 'Marandu' [(Urochloa brizantha (Syn. Brachiaria brizantha)] cultivadas em sistemas agrossilvipastoris. O experimento foi estabelecido em condições de campo, em um delineamento de blocos ao acaso, com quatro repetições, em que as parcelas foram representadas pelos espaçamentos de plantio de eucalipto (12x2 e 12x4 m), e as subparcelas, pela distância entre o capim 'Marandu' e as fileiras de eucalipto (6, 4 e 2 m). Adicionou-se um controle apenas com capim 'Marandu', cultivado a sol pleno. As variáveis de anatomia foliar do capim 'Marandu' foram avaliadas quanto à interferência do sombreamento pelas árvores. A distância entre as plantas de capim 'Marandu' e as linhas de eucalipto interferiram na anatomia da lâmina foliar da forragem, e as maiores modificações ocorreram nas medidas lineares e proporções teciduais nos arranjos com maior densidade de eucalipto. A interferência do sombreamento pelo eucalipto nas características anatômicas das folhas de capim 'Marandu' depende do arranjo adotado. Isto ocorre em razão da adaptação do capim à baixa incidência de luz, com a consequente redução de sua atividade fisiológica e crescimento.

Abstract: The objective of this work was to evaluate the effects of different arrangements of eucalyptus plants on the morphoanatomical characteristics of leaf blades of 'Marandu' grass [(Urochloa brizantha (Syn. Brachiaria brizantha)] plants cultivated in agrosilvopastoral systems. The experiment was set in field conditions in a randomized complete block design with four replicates, in which the plots were represented by planting spacings of eucalyptus (12x2 and 12x4 m), and the subplots, by the distance between 'Marandu' grass and eucalyptus rows (6, 4, and 2 m). A control was added, composed by 'Marandu' grass cultivated in full sun. Variables of the leaf anatomy of 'Marandu' grass were evaluated for shading interference by trees. The distance of 'Marandu' grass plants from the eucalyptus lines interfered with the leaf blade anatomy of the forage, and the highest modifications occurred in linear measures and tissue proportions in the arrangements with the highest eucalyptus density. The shading interference of eucalyptus on the anatomical characteristics of leaf blades of 'Marandu' grass depends on the adopted arrangement. This occurs due to the grass adaptation to the low-light incidence, with a consequent reduction in its physiological activity and growth.