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Abstract Objective: To investigate the usefulness, reliability, quality, and content of Portuguese-language YouTube videos on COVID-19. Material and Methods: Three keywords selected on Google Trends were searched on YouTube, and the 60 first videos listed with each term were analyzed. Two calibrated researchers evaluated the reliability (DISCERN Modified Scale), the quality (Global Quality Score - GQS), and the usefulness of videos for the users (COVID-19 Specific Score - CSS). The number of views, likes, and engagement were also analyzed. The data were evaluated by the Mann-Whitney test and Spearman correlation (α=5%). Results: 59 videos were included. The average scores of quality, reliability, and usefulness were 3.0 (±1.1), 3.2 (± 0.8), and 1.5 (± 0.9), respectively. Two-thirds of the videos (64.4%) had low/moderate quality, and the majority (76.2%) were about signs and symptoms. The numbers of views (p=0.005), likes (p=0.006), and engagement (p=0.014) were significantly higher in moderate/good quality videos. The number of comments (p=0.007), duration of videos (p=0.004), and the DISCERN score (p<0.001) were significantly higher in videos made by health professionals. The general quality of the videos was positively correlated to the CSS scores, number of views, likes, and engagement (p<0.05). Conclusion: Most videos had moderate quality and reliability and low usefulness for the users.

https://doi.org/10.1590/pboci.2024.009

Clinical characteristics and outcomes of patients with COVID-19 admitted to the intensive care unit during the first and second waves of the pandemic in Brazil: a single-center retrospective cohort study
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Corrêa, Thiago Domingos

; Midega, Thais Dias

; Cordioli, Ricardo Luiz

; Barbas, Carmen Silvia Valente

; Rabello Filho, Roberto

; Silva, Bruno Caldin da

; Silva Júnior, Moacyr

; Nawa, Ricardo Kenji

; Carvalho, Fabrício Rodrigues Torres de

; Matos, Gustavo Faissol Janot de

; Lucinio, Neide Marcela

; Rodrigues, Rodrigo Dias

; Eid, Raquel Afonso Caserta

; Bravim, Bruno de Arruda

; Pereira, Adriano José

; Santos, Bento Fortunato Cardoso dos

; Pinho, João Renato Rebello

; Pardini, Andreia

; Teich, Vanessa Damazio

; Laselva, Claudia Regina

; Cendoroglo Neto, Miguel

; Klajner, Sidney

; Ferraz, Leonardo José Rolim

einstein (São Paulo)

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2023, Volume 21 elocation eAO0233

Abstract: En | Text: En | PDF: En

ABSTRACT Objective To describe and compare the clinical characteristics and outcomes of patients admitted to intensive care units during the first and second waves of the COVID-19 pandemic. Methods In this retrospective single-center cohort study, data were retrieved from the Epimed Monitor System; all adult patients admitted to the intensive care unit between March 4, 2020, and October 1, 2021, were included in the study. We compared the clinical characteristics and outcomes of patients admitted to the intensive care unit of a quaternary private hospital in São Paulo, Brazil, during the first (May 1, 2020, to August 31, 2020) and second (March 1, 2021, to June 30, 2021) waves of the COVID-19 pandemic. Results In total, 1,427 patients with COVID-19 were admitted to the intensive care unit during the first (421 patients) and second (1,006 patients) waves. Compared with the first wave group [median (IQR)], the second wave group was younger [57 (46-70) versus 67 (52-80) years; p<0.001], had a lower SAPS 3 Score [45 (42-52) versus 49 (43-57); p<0.001], lower SOFA Score on intensive care unit admission [3 (1-6) versus 4 (2-6); p=0.018], lower Charlson Comorbidity Index [0 (0-1) versus 1 (0-2); p<0.001], and were less frequently frail (10.4% versus 18.1%; p<0.001). The second wave group used more noninvasive ventilation (81.3% versus 53.4%; p<0.001) and high-flow nasal cannula (63.2% versus 23.0%; p<0.001) during their intensive care unit stay. The intensive care unit (11.3% versus 10.5%; p=0.696) and in-hospital mortality (12.3% versus 12.1%; p=0.998) rates did not differ between both waves. Conclusion In the first and second waves, patients with severe COVID-19 exhibited similar mortality rates and need for invasive organ support, despite the second wave group being younger and less severely ill at the time of intensive care unit admission.

https://doi.org/10.31744/einstein_journal/2023ao0233

COVID-19-associated coagulopathy and acute kidney injury in critically ill patients
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Silva, Bruno Caldin da

; Cordioli, Ricardo Luiz

; Santos, Bento Fortunato Cardoso dos

; Guerra, João Carlos de Campos

; Rodrigues, Roseny dos Reis

; Souza, Guilherme Martins de

; Ashihara, Carolina

; Midega, Thais Dias

; Campos, Niklas Söderberg

; Carneiro, Bárbara Vieira

; Campos, Flávia Nunes Dias

; Guimarães, Hélio Penna

; Matos, Gustavo Faissol Janot de

; Aranda, Valdir Fernandes de

; Ferraz, Leonardo José Rolim

; Corrêa, Thiago Domingos

einstein (São Paulo)

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2023, Volume 21 elocation eAO0119

Abstract: En | Text: En | PDF: En

ABSTRACT Objective The incidence of thrombotic events and acute kidney injury is high in critically ill patients with COVID-19. We aimed to evaluate and compare the coagulation profiles of patients with COVID-19 developing acute kidney injury versus those who did not, during their intensive care unit stay. Methods Conventional coagulation and platelet function tests, fibrinolysis, endogenous inhibitors of coagulation tests, and rotational thromboelastometry were conducted on days 0, 1, 3, 7, and 14 following intensive care unit admission. Results Out of 30 patients included, 13 (43.4%) met the criteria for acute kidney injury. Comparing both groups, patients with acute kidney injury were older: 73 (60-84) versus 54 (47-64) years, p=0.027, and had a lower baseline glomerular filtration rate: 70 (51-81) versus 93 (83-106) mL/min/1.73m2, p=0.004. On day 1, D-dimer and fibrinogen levels were elevated but similar between groups: 1780 (1319-5517) versus 1794 (726-2324) ng/mL, p=0.145 and 608 (550-700) versus 642 (469-722) g/dL, p=0.95, respectively. Rotational thromboelastometry data were also similar between groups. However, antithrombin activity and protein C levels were lower in patients who developed acute kidney injury: 82 (75-92) versus 98 (90-116), p=0.028 and 70 (52-82) versus 88 (78-101) µ/mL, p=0.038, respectively. Mean protein C levels were lower in the group with acute kidney injury across multiple time points during their stay in the intensive care unit. Conclusion Critically ill patients experiencing acute kidney injury exhibited lower endogenous anticoagulant levels. Further studies are needed to understand the role of natural anticoagulants in the pathophysiology of acute kidney injury within this population.

https://doi.org/10.31744/einstein_journal/2023ao0119

The SISBIOTA-Diptera Brazilian Network: A long-term survey of Diptera from unexplored Brazilian Western Arc of Amazon, Cerrado, and Pantanal
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Lamas, Carlos José Einicker

; Fachin, Diego Aguilar

; Falaschi, Rafaela Lopes

; Alcantara, Daniel Máximo Correa de

; Ale-Rocha, Rosaly

; Amorim, Dalton de Souza

; Araújo, Maíra Xavier

; Ascendino, Sharlene

; Baldassio, Letícia

; Bellodi, Carolina Ferraz

; Bravo, Freddy

; Calhau, Julia

; Capellari, Renato Soares

; Carmo-Neto, Antonio Marcelino do

; Cegolin, Bianca Melo

; Couri, Márcia Souto

; Carvalho, Claudio José Barros de

; Dios, Rodrigo de Vilhena Perez

; Falcon, Aida Vanessa Gomez

; Fusari, Livia Maria

; Garcia, Carolina de Almeida

; Gil-Azevedo, Leonardo Henrique

; Gomes, Marina Morim

; Graciolli, Gustavo

; Gudin, Filipe Macedo

; Henriques, Augusto Loureiro

; Krolow, Tiago Kütter

; Mendes, Luanna Layla

; Limeira-de-Oliveira, Francisco

; Maia, Valéria Cid

; Marinoni, Luciane

; Mello, Ramon Luciano

; Mello-Patiu, Cátia Antunes de

; Morales, Mírian Nunes

; Oliveira, Sarah Siqueira

; Patiu, Claudemir

; Proença, Barbara

; Pujol-Luz, Cristiane Vieira de Assis

; Pujol-Luz, José Roberto

; Rafael, José Albertino

; Riccardi, Paula Raile

; Rodrigues, João Paulo Vinicios

; Roque, Fabio de Oliveira

; Sallum, Maria Anice Mureb

; Santis, Marcelo Domingos de

; Santos, Charles Morphy Dias dos

; Santos, Josenilson Rodrigues dos

; Savaris, Marcoandre

; Shimabukuro, Paloma Helena Fernandes

; Silva, Vera Cristina

; Schelesky-Prado, Daniel de Castro

; Silva-Neto, Alberto Moreira da

; Camargo, Alexssandro

; Sousa, Viviane Rodrigues de

; Urso-Guimarães, Maria Virginia

; Wiedenbrug, Sofia

; Yamaguchi, Carolina

; Nihei, Silvio Shigueo

Revista Brasileira de Entomologia

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2023, Volume 67 N. 4 elocation e20230051

Abstract: En | Text: En | PDF: En

ABSTRACT The SISBIOTA-BRASIL was a three-year multimillion-dollar research program of the Brazilian government to document plants and animals in endangered/understudied areas and biomes in Brazil. Distributional patterns and the historical events that generated them are extensively unknown regarding Brazilian fauna and flora. This deficiency hinders the development of conservation policies and the understanding of evolutionary processes. Conservation decisions depend on precise knowledge of the taxonomy and geographic distribution of species. Given such a premise, we proposed to research the diversity of Diptera of the Brazilian western arc of Amazon, Cerrado, and Pantanal in the states of Mato Grosso, Mato Grosso do Sul, and Rondônia. Three important biomes of the South American continent characterize these Brazilian states: Amazon forest, Cerrado (Brazilian Savannah), and Pantanal. Besides their ecological relevance, these biomes historically lack intensive entomological surveys. Therefore, they are much underrepresented in the Brazilian natural history collections and in the scientific literature, which is further aggravated by the fact that these areas are being exponentially and rapidly converted to commercial lands. Our project involved over 90 collaborators from 24 different Brazilian institutions and one from Colombia among researchers, postdocs, graduate and undergraduate students, and technicians. We processed and analyzed nearly 300,000 specimens from ~60 families of Diptera collected with a large variety of methods in the sampled areas. Here, we provide a detailed overview of the genera and species diversity of 41 families treated. Our results point to a total of 2,130 species and 514 genera compiled and identified for the three states altogether, with an increase of 41% and 29% in the numbers of species and genera known for the three states combined, respectively. Overall, the 10 most species-rich families were Tachinidae, Cecidomyiidae, Tabanidae, Psychodidae, Sarcophagidae, Stratiomyidae, Bombyliidae, Syrphidae, Tephritidae, and Asilidae. The 10 most diverse in the number of genera were Tachinidae, Stratiomyidae, Asilidae, Mycetophilidae, Syrphidae, Tabanidae, Muscidae, Dolichopodidae, Sarcophagidae, and Chloropidae. So far, 111 scientific papers were published regarding taxonomic, phylogenetic, and biogeographical aspects of the studied families, with the description of 101 new species and three new genera. We expect that additional publications will result from this investigation because several specimens are now curated and being researched by specialists.

https://doi.org/10.1590/1806-9665-rbent-2023-0051

Novel pulp capping material based on sodium trimetaphosphate: synthesis, characterization, and antimicrobial properties
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Franzin, Nayara Rodrigues Sartori

; Sostena, Michela Melissa Duarte Seixas

; Santos, Alailson Domingos dos

; Moura, Marcia Regina

; Camargo, Emerson Rodrigues de

; Hosida, Thayse Yumi

; Delbem, Alberto Carlos Botazzo

; Moraes, João Carlos Silos

Journal of Applied Oral Science

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2022, Volume 30 elocation e20210483

Abstract: En | Text: En | PDF: En

Abstract Objectives: To evaluate the mechanical, physicochemical, and antimicrobial properties of four different formulations containing micro- or nanoparticles of sodium trimetaphosphate (mTMP and nTMP, respectively). Methodology: Four experimental groups were used in this investigation: two mTMP groups and two nTMP groups, each containing zirconium oxide (ZrO2), and solution containing either chitosan or titanium oxide (TiO2) nanoparticles (NPs). Setting time, compression resistance, and radiopacity were estimated. The agar diffusion test was used to assess the antimicrobial activity of the formulations against five different microbial strains: Streptococcus mutans, Lactobacillus casei, Actinomyces israelii, Candida albicans, and Enterococcus faecalis. Parametric and nonparametric tests were performed after evaluating homoscedasticity data (p<0.05). Results: From the properties evaluated, nTMP cements required less setting time and showed greater resistance to compression. Cements containing TiO2 showed greater radiopacity for both nTMP and mTMP. All four cement formulations showed antimicrobial activity against S. mutans and L. casei Conclusion: Formulations containing nTMP have shorter setting times and higher compressive strength, and those with TiO2 nanoparticles showed antimicrobial activities. Clinical relevance: The cement containing nTMP, ZrO2, and TiO2 could be an alternative material for protecting the pulp complex.

https://doi.org/10.1590/1678-7757-2021-0483

Foliar application of salicylic acid intensifies antioxidant system and photosynthetic efficiency in tomato plants
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Aires, Eduardo Santana

; Ferraz, Andrew Kim Lopes

; Carvalho, Beatriz Lívero

; Teixeira, Fabrício Palla

; Rodrigues, João Domingos

; Ono, Elizabeth Orika

Bragantia

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2022, Volume 81 elocation e1522

Abstract: En | Text: En | PDF: En

ABSTRACT Tomatoes are the most important and grown vegetable crop in the world. The salicylic acid (SA) application could improve crop yields due the positive physiological effects of this plant growth regulator. Thus, this study aimed to evaluate the possible effects of SA application on leaf regarding the intensification of antioxidant enzymes activities, chlorophyll a fluorescence, gas exchange, and tomato production against environmental stress. This experiment was conducted by the use of Colossal tomato hybrid in a protected environment between July and December 2019. Therefore, a randomized block design with five SA doses was used, as follows: 0; 0.5; 1; 1.5, and 2 mM. Then, applications were performed weekly from 15 to 60 days after transplantation (DAT). At the 45th and the 60th DAT, the enzymes activities were analysed, such as superoxide dismutase (SOD), catalase activity (CAT) and peroxidase (POD), lipid peroxidation, proline content, chlorophyll a fluorescence, gas exchange, and plant height. At the end of the experiment, fruit weight, total and commercial production were also evaluated. Results indicated that foliar application of SA reduced the environmental stress in plants through the intensification of antioxidant system that reduced lipid peroxidation and qNP and increased the efficiency of photosystem II and ETR. Furthermore, gas exchange was also influenced by the action of SA in gs, favouring A and A/Ci. The SA dose between 0.5 and 0.8 mM positively enabled the total and commercial production of tomatoes. Therefore, foliar application of SA reduced oxidative damage, and increased photosynthetic efficiency and fruit production.

https://doi.org/10.1590/1678-4499.20210320

The Program for Biodiversity Research in Brazil: The role of regional networks for biodiversity knowledge, dissemination, and conservation
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ROSA, CLARISSA

; BACCARO, FABRICIO

; CRONEMBERGER, CECILIA

; HIPÓLITO, JULIANA

; BARROS, CLAUDIA FRANCA

; RODRIGUES, DOMINGOS DE JESUS

; NECKEL-OLIVEIRA, SELVINO

; OVERBECK, GERHARD E.

; DRECHSLER-SANTOS, ELISANDRO RICARDO

; ANJOS, MARCELO RODRIGUES DOS

; FERREGUETTI, ÁTILLA C.

; AKAMA, ALBERTO

; MARTINS, MARLÚCIA BONIFÁCIO

; TOMAS, WALFRIDO MORAES

; SANTOS, SANDRA APARECIDA

; FERREIRA, VANDA LÚCIA

; CUNHA, CATIA NUNES DA

; PENHA, JERRY

; PINHO, JOÃO BATISTA DE

; SALIS, SUZANA MARIA

; DORIA, CAROLINA RODRIGUES DA COSTA

; PILLAR, VALÉRIO D.

; PODGAISKI, LUCIANA R.

; MENIN, MARCELO

; BÍGIO, NARCÍSIO COSTA

; ARAGÓN, SUSAN

; MANZATTO, ANGELO GILBERTO

; VÉLEZ-MARTIN, EDUARDO

; SILVA, ANA CAROLINA BORGES LINS E

; IZZO, THIAGO JUNQUEIRA

; MORTATI, AMANDA FREDERICO

; GIACOMIN, LEANDRO LACERDA

; ALMEIDA, THAÍS ELIAS

; ANDRÉ, THIAGO

; SILVEIRA, MARIA AUREA PINHEIRO DE ALMEIDA

; SILVEIRA, ANTÔNIO LAFFAYETE PIRES DA

; MESSIAS, MARILUCE REZENDE

; MARQUES, MARCIA C.M.

; PADIAL, ANDRE ANDRIAN

; MARQUES, RENATO

; BITAR, YOUSZEF O.C.

; SILVEIRA, MARCOS

; MORATO, ELDER FERREIRA

; PAGOTTO, RUBIANI DE CÁSSIA

; STRUSSMANN, CHRISTINE

; MACHADO, RICARDO BOMFIM

; AGUIAR, LUDMILLA MOURA DE SOUZA

; FERNANDES, GERALDO WILSON

; OKI, YUMI

; NOVAIS, SAMUEL

; FERREIRA, GUILHERME BRAGA

; BARBOSA, FLÁVIA RODRIGUES

; OCHOA, ANA C.

; MANGIONE, ANTONIO M.

; GATICA, AILIN

; CARRIZO, MARÍA CELINA

; RETTA, LUCÍA MARTINEZ

; JOFRÉ, LAURA E.

; CASTILLO, LUCIANA L.

; NEME, ANDREA M.

; RUEDA, CARLA

; TOLEDO, JOSÉ JULIO DE

; GRELLE, CARLOS EDUARDO VIVEIROS

; VALE, MARIANA M.

; VIEIRA, MARCUS VINICIUS

; CERQUEIRA, RUI

; HIGASHIKAWA, EMÍLIO MANABU

; MENDONÇA, FERNANDO PEREIRA DE

; GUERREIRO, QUÊZIA LEANDRO DE MOURA

; BANHOS, AUREO

; HERO, JEAN-MARC

; KOBLITZ, RODRIGO

; COLLEVATTI, ROSANE GARCIA

; SILVEIRA, LUÍS FÁBIO

; VASCONCELOS, HERALDO L.

; VIEIRA, CECÍLIA RODRIGUES

; COLLI, GUARINO RINALDI

; CECHIN, SONIA ZANINI

; SANTOS, TIAGO GOMES DOS

; FONTANA, CARLA S.

; JARENKOW, JOÃO A.

; MALABARBA, LUIZ R.

; RUEDA, MARTA P.

; ARAUJO, PUBLIO A.

; PALOMO, LUCAS

; ITURRE, MARTA C.

; BERGALLO, HELENA GODOY

; MAGNUSSON, WILLIAM E.

Anais da Academia Brasileira de Ciências

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2021, Volume 93 N. 2 elocation e20201604

Abstract: En | Text: En | PDF: En

Abstract The Program for Biodiversity Research (PPBio) is an innovative program designed to integrate all biodiversity research stakeholders. Operating since 2004, it has installed long-term ecological research sites throughout Brazil and its logic has been applied in some other southern-hemisphere countries. The program supports all aspects of research necessary to understand biodiversity and the processes that affect it. There are presently 161 sampling sites (see some of them at Supplementary Appendix), most of which use a standardized methodology that allows comparisons across biomes and through time. To date, there are about 1200 publications associated with PPBio that cover topics ranging from natural history to genetics and species distributions. Most of the field data and metadata are available through PPBio web sites or DataONE. Metadata is available for researchers that intend to explore the different faces of Brazilian biodiversity spatio-temporal variation, as well as for managers intending to improve conservation strategies. The Program also fostered, directly and indirectly, local technical capacity building, and supported the training of hundreds of undergraduate and graduate students. The main challenge is maintaining the long-term funding necessary to understand biodiversity patterns and processes under pressure from global environmental changes.

https://doi.org/10.1590/0001-3765202120201604 1034 downloads

Combining ability of physiological traits in forrage maize
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Redivo, Greice Daiane Rodrigues Gomes

; Faria, Marcos Ventura

; Rosa, Jocimar Costa

; Ono, Elizabeth Orika

; Rodrigues, João Domingos

; Adriano, Elisa

Ciência e Agrotecnologia

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2020, Volume 44 elocation e018520

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A exploração da variabilidade genética existente para características fisiológicas relacionadas com a produtividade e a qualidade da forragem de milho pode auxiliar no desenvolvimento de linhagens e híbridos superiores. O objetivo deste trabalho foi avaliar a capacidade geral e específica de combinação e a natureza dos efeitos genicos de características fisiológicas e químicas de genótipos de milho forrageiro. Foram avaliados dois grupos de genótipos e 16 híbridos resultantes dos cruzamentos em esquema de dialelo parcial 4x4. O grupo I foi constituído por dois híbridos comerciais (AG8025, P30B39) e duas linhagens elite (LEM2 e LEM3). O grupo II foi constituído por quatro linhagens experimentais oriundas de diferentes populações do programa de melhoramento do milho forrageiro da UNICENTRO. No total foram avaliados 24 tratamentos, constituídos pelos genótipos de ambos os grupos e os respectivos cruzamentos. As características avaliadas foram assimilação de CO2 (A), condutância estomática (gs), concentração interna de CO2 (Ci), transpiração (E), atividade calculada da Rubisco (A/Ci) e eficiência de uso da água (A/E). Foram obtidos os teores de fibra em detergente ácido (FDA), fibra em detergente neutro (FDN) e a digestibilidade in situ da forragem. Houve predomínio de efeitos gênicos não aditivos para a maioria das características químicas e fisiológicas. Os cruzamentos LEM2 x 203-218,3, LEM3 x 201-107,2, LEM2 x 101-7,2 e LEM3 x 101-7,2 se destacaram quanto à assimilação de CO2, sendo indicados para pesquisas futuras considerando as características fisiológicas. As linhagens 101-7.2 e 203-218.3 apresentaram alta concentração de alelos favoráveis para aumentar a eficiência da carboxilação, em que a linhagem 101-7.2 se destacou para NDF, ADF e DIG. A linhagem endogâmica 201-107.2 possui alta concentração de alelos favoráveis para eficiência do uso da água. Parâmetros fisiológicos podem auxiliar na seleção de linhagens e híbridos no melhoramento do milho forrageiro.

ABSTRACT The exploitation of the existing genetic variability for the physiological traits related to the yield and quality of maize forage can assist in the development of superior inbred lines and hybrids. The objective of this work was to evaluate the general and the specific combining ability and the nature of gene effects of physiological and chemical traits of forage maize genotypes. Two groups of genotypes and 16 hybrids resulting from a 4x4 partial diallel scheme were evaluated. Group I consisted of two commercial hybrids (AG8025, P30B39) and two elite inbred lines (LEM2 and LEM3). Group II consisted of four experimental inbred lines originated from different populations of forage maize breeding program. In total, 24 treatments were evaluated, formed by the genotypes of both groups and the respective crossings. The traits evaluated were: CO2 assimilation (A), stomatal conductance (gs), internal CO2 concentration (Ci), transpiration (E), calculated activity of Rubisco (A/Ci) and efficiency of water use (A/E). Forage acid detergent fiber (ADF), neutral detergent fiber (NDF) and digestibility in situ were obtained. There was a predominance of non-additive gene effects for most of the chemical and physiological traits. Crossings LEM2 x 203-218.3, LEM3 x 201-107.2, LEM2 x 101-7.2 and LEM3 x 101-7.2 stood out regarding CO2 assimilation, and are indicated for future research considering the physiological traits. Inbred lines 101-7.2 and 203-218.3 presented high concentration of favorable alleles to increase carboxylation efficiency, in which inbred line 101-7.2 stood out for NDF, ADF and DIG. Inbred line 201-107.2 has a high concentration of favorable alleles for efficiency of water use. Physiological parameters can assist the selection of inbred lines and hybrids in maize breeding for forage purpose.

https://doi.org/10.1590/1413-7054202044018520 495 downloads

Intensive support recommendations for critically-ill patients with suspected or confirmed COVID-19 infection
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Corrêa, Thiago Domingos

; Matos, Gustavo Faissol Janot de

; Bravim, Bruno de Arruda

; Cordioli, Ricardo Luiz

; Garrido, Alejandra del Pilar Gallardo

; Assuncao, Murillo Santucci Cesar de

; Barbas, Carmen Silvia Valente

; Timenetsky, Karina Tavares

; Rodrigues, Roseny dos Reis

; Guimarães, Hélio Penna

; Rabello Filho, Roberto

; Lomar, Frederico Polito

; Scarin, Farah Christina de La Cruz

; Batista, Carla Luciana

; Pereira, Adriano José

; Guerra, João Carlos de Campos

; Carneiro, Bárbara Vieira

; Nawa, Ricardo Kenji

; Brandão, Rodrigo Martins

; Pesaro, Antônio Eduardo Pereira

; Silva Júnior, Moacyr

; Carvalho, Fabricio Rodrigues Torres de

; Silva, Cilene Saghabi de Medeiros

; Almeida, Ana Claudia Ferraz de

; Franken, Marcelo

; Pesavento, Marcele Liliane

; Eid, Raquel Afonso Caserta

; Ferraz, Leonardo José Rolim

einstein (São Paulo)

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2020, Volume 18 elocation eAE5793

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Em dezembro de 2019, uma série de pacientes com pneumonia grave foi identificada em Wuhan, província de Hubei, na China. Esses pacientes evoluíram para síndrome respiratória aguda grave e síndrome do desconforto respiratório agudo. Posteriormente, a COVID-19 foi atribuída a um novo betacoronavírus, o coronavírus da síndrome respiratória aguda grave 2 (SARS-CoV-2). Cerca de 20% dos pacientes com diagnóstico de COVID-19 desenvolvem formas graves da doença, incluindo insuficiência respiratória aguda hipoxêmica, síndrome respiratória aguda grave, síndrome do desconforto respiratório agudo e insuficiência renal aguda e requerem admissão em unidade de terapia intensiva. Não há nenhum ensaio clínico randomizado controlado que avalie potenciais tratamentos para pacientes com infecção confirmada pela COVID-19 no momento da publicação destas recomendações de tratamento. Dessa forma, essas recomendações são baseadas predominantemente na opinião de especialistas (grau de recomendação de nível C).

ABSTRACT In December 2019, a series of patients with severe pneumonia were identified in Wuhan, Hubei province, China, who progressed to severe acute respiratory syndrome and acute respiratory distress syndrome. Subsequently, COVID-19 was attributed to a new betacoronavirus, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Approximately 20% of patients diagnosed as COVID-19 develop severe forms of the disease, including acute hypoxemic respiratory failure, severe acute respiratory syndrome, acute respiratory distress syndrome and acute renal failure and require intensive care. There is no randomized controlled clinical trial addressing potential therapies for patients with confirmed COVID-19 infection at the time of publishing these treatment recommendations. Therefore, these recommendations are based predominantly on the opinion of experts (level C of recommendation).

https://doi.org/10.31744/einstein_journal/2020ae5793 23244 downloads

10.

Comment to: Intensive support recommendations for critically-ill patients with suspected or confirmed COVID-19 infection
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Corrêa, Thiago Domingos

; Matos, Gustavo Faissol Janot de

; Bravim, Bruno de Arruda

; Cordioli, Ricardo Luiz

; Garrido, Alejandra del Pilar Gallardo

; Assuncao, Murillo Santucci Cesar de

; Barbas, Carmen Silvia Valente

; Timenetsky, Karina Tavares

; Rodrigues, Roseny dos Reis

; Guimarães, Hélio Penna

; Rabello Filho, Roberto

; Lomar, Frederico Polito

; Scarin, Farah Christina de La Cruz

; Batista, Carla Luciana

; Pereira, Adriano José

; Guerra, João Carlos de Campos

; Carneiro, Bárbara Vieira

; Nawa, Ricardo Kenji

; Brandão, Rodrigo Martins

; Pesaro, Antônio Eduardo Pereira

; Silva Júnior, Moacyr

; Carvalho, Fabricio Rodrigues Torres de

; Silva, Cilene Saghabi de Medeiros

; Almeida, Ana Claudia Ferraz de

; Franken, Marcelo

; Pesavento, Marcele Liliane

; Eid, Raquel Afonso Caserta

; Ferraz, Leonardo José Rolim

einstein (São Paulo)

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2020, Volume 18 elocation eCE5931

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.31744/einstein_journal/2020ce5931 214 downloads

11.

Concentrações de biorreguladores na germinação e emergência de jiló
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Jorge, Leticia Galhardo

; Torres, Thaís Prado

; Martins, Bruno Novaes Menezes

; Ono, Elizabeth Orika

; Rodrigues, João Domingos

Revista de Ciências Agrárias

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sep 2019, Volume 42 N. 3 Pages 121 - 130

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Objetivou-se avaliar o efeito de diferentes concentrações de biorreguladores na germinação e emergência de plântulas de jiló. Foram realizados dois experimentos independentes, sendo o primeiro experimento em câmara tipo BOD, avaliando-se a germinação e o vigor de sementes em função de diferentes concentrações de reguladores vegetais aplicadas via semente, sendo: 0; 100; 200 e 300 mg.L-1 de GA3; 0; 50; 100 e 150 mg L-1 da mistura de GA4+7 + benziladenina e 0; 0,001; 0,05 e 0,1 ppm de brassinolídeo. O segundo experimento foi realizado em casa de vegetação, sendo os tratamentos aplicados os mesmos utilizados no primeiro experimento, determinando-se a emergência de sementes de jiló. O delineamento experimental utilizado nos dois experimentos foi inteiramente casualizado, com 4 repetições de 40 sementes. A aplicação via sementes com concentração próxima de 300 mg.L-1 de GA3 e 150 mg.L-1 de GA4+7 + benziladenina, aumentou a porcentagem de germinação e o índice de velocidade de germinação em jiló. As concentrações de brassinolídeo não influenciou a germinação de sementes. O uso de biorreguladores não contribuiu nas características de emergência de plântulas em casa de vegetação.

The objective of this work was to evaluate the effect of different concentrations of bioregulators on the germination and emergence of jiló seedlings. Two independent experiments were carried out, being the first experiment in chamber type BOD, evaluating the germination and seed vigor as a function of different concentrations of plant regulators applied via seed, being: 0; 100; 200 and 300 mg.L-1 GA3; 0; 50; 100 and 150 mg.L-1 of the mixture of GA4+7 + benzyladenine and 0; 0.001; 0.05 and 0.1 ppm brassinolide. The second experiment was conducted in a greenhouse, and the treatments were the same as those used in the first experiment, determining the emergence of jiló seeds. The experimental design used in the two experiments was completely randomized, with 4 replicates of 40 seeds. The application via seeds with a concentration of approximately 300 mg.L-1 of GA3 and 150 mg.L-1 of GA4+7 + benzyladenine, increased the percentage of germination and the rate of germination speed in jiló. Brassinolide concentrations did not influence seed germination. The use of bioregulators did not contribute to the emergence characteristics of greenhouse seedlings.

https://doi.org/10.19084/rca.17478 560 downloads

12.

Environmental structure affects taxonomic diversity but not functional structure of understory birds in the southwestern Brazilian Amazon
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OLIVEIRA, Jocieli de

; ALMEIDA, Sara M.

; FLORÊNCIO, Fernando P.

; PINHO, João B.

; OLIVEIRA, Dalci M. M.

; LIGEIRO, Raphael

; RODRIGUES, Domingos J.

Acta Amazonica

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sep 2019, Volume 49 N. 3 Pages 232 - 241

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Mudanças nas características ambientais podem influenciar o uso do habitat por aves, afetando o número de indivíduos, a riqueza de espécies e alterando a composição de espécies e estrutura funcional das assembleias. Medidas que avaliam a estrutura funcional de assembléias biológicas constituem uma ferramenta complementar à abordagem taxonômica, pois quantificam as diferenças entre as espécies através de seus atributos funcionais. Nós acessamos o efeito de variáveis ambientais sobre a diversidade taxonômica (riqueza, composição de espécies e número de indivíduos) e estrutura funcional (riqueza funcional, equabilidade funcional, diversidade funcional e média dos atributos ponderada pelas abundâncias) de assembleias de aves no norte do estado de Mato Grosso. Amostramos aves em 32 parcelas. Em cada parcela as aves foram capturadas usando redes de neblina e oito variáveis ambientais foram mensuradas: abertura de dossel, volume de serapilheira, elevação, número de árvores em três classe de DAP, teor de argila no solo e distância ao riacho mais próximo. Para avaliar a estrutura funcional nós tomamos sete medidas morfológicas de cada espécie de aves. Encontramos um efeito significativo das variáveis do habitat sobre a diversidade taxonômica, porém, a estrutura funcional, de maneira geral, não foi afetada. Elevação e distância ao riacho mais próximo foram as variáveis que direcionaram as mudanças na diversidade taxonômica e tiveram um pequeno efeito na riqueza funcional. As demais métricas de estrutura funcional não foram afetadas significativamente pelo conjunto de variáveis ambientais. Nossos resultados sugerem que a assembleia estudada exibe certa tolerância funcional (redundância) à variação ambiental em pequena escala, implicando em alguma resiliência à modificação do ecossistema.

ABSTRACT Changes in environmental characteristics can affect habitat use by birds, impacting the number of individuals, number of species, and changing species composition and functional structure of assemblages. Metrics that evaluate the functional structure of biological assemblages constitute a complementary tool to the traditional taxonomic approach, because they quantify the differences between species by means of functional traits. We assessed the effect of environmental characteristics on the taxonomic diversity (species richness, species composition and number of individuals) and functional structure (functional richness, functional evenness, Rao’s functional diversity, and community-weighted mean traits) of bird assemblages in northern Mato Grosso state, in the southwestern Brazilian Amazon. We sampled birds in 32 plots. At each plot birds were captured using mist nets, and eight environmental variables were measured: canopy openness, leaf litter, elevation, number of trees in three classes of DBH, soil clay content, and distance to nearest stream. To evaluate functional structure, we measured seven morphological traits from individuals of each bird species. Habitat variables had a significant effect on taxonomic diversity. However, the general functional structure was not affected. Elevation and distance to nearest stream were the main variables driving changes in taxonomic diversity and had a minor effect on functional richness. The other metrics of functional structure were not significantly affected by the set of environmental variables. Our results suggest that the sampled bird assemblages exhibit some functional tolerance (redundancy) to small-scale environmental variation, implying certain resilience to ecosystem modification.

https://doi.org/10.1590/1809-4392201803941 1500 downloads

13.

Ácido salicílico e acibenzolar-S-methyl como atenuadores de fitointoxicação causada pelo chlorimuron-ethyl na cultura da soja
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Dias, Roque de Carvalho

; Bianchi, Leandro

; Batista, Thiago Barbosa

; Germino, Gabriel Henrique

; Carbonari, Caio Antonio

; Ono, Elizabeth Orika

; Rodrigues, João Domingos

; Velini, Edivaldo Domingues

Revista de Ciências Agrárias

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jun 2019, Volume 42 N. 2 Pages 141 - 150

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O ácido salicílico (AS) e o acibenzolar-S-methyl (ASM) podem ser usados como atenuadores de estresses bióticos/abióticos causados em plantas, entretanto, são escassos os estudos visando minimizar os possíveis efeitos negativos da utilização de herbicidas em plantas. Diante disso, objetivou-se avaliar o efeito do AS e ASM como atenuadores de fitointoxicação causado pelo chlorimuron-ethyl na cultura da soja. Foi realizado um experimento em casa de vegetação no delineamento em blocos casualizados com cinco repetições. Os tratamentos aplicados no estádio V3-V4 na cultura da soja foram: controle (sem herbicida, sem AS e ASM); AS (1,0 mM); ASM (1,0 mM); AS 7 dias antes da aplicação do herbicida + chlorimuron-ethyl (1,0 mM + 100 g ha-1); ASM 7 dias antes a aplicação do herbicida + chlorimuron-ethyl (1,0 mM + 100 g ha-1, respectivamente); chlorimuron-ethyl (100 g ha-1); AS em mistura de calda + chlorimuron-ethyl (1,0 mM + 100 g ha-1, respectivamente) e ASM em mistura de calda + chlorimuron-ethyl (1,0 mM + 100 g ha-1, respectivamente). Decorrido três horas após a aplicação e aos 3, 7, 14, 21 e 28 dias depois da aplicação (DDA) foram realizadas avaliações de taxa de transporte de elétrons (TTE). Nesses mesmos dias avaliou-se os sintomas de fitointoxicação e a estimativa da área foliar (AF). Aos 28 DDA mediu-se a altura das plantas e a biomassa seca da parte aérea. A aplicação de AS e ASM, antes ou em mistura com herbicida chlorimuron-ethyl, reduziu os sintomas de fitointoxicação, manteve os índices de TTE próximos ao tratamento controle e minimizou as reduções da AF, altura e biomassa seca. A aplicação de AS e ASM atenuou o estresse causado pelo chlorimuron-ethyl na soja.

Salicylic acid (SA) and acibenzolar-S-methyl (ASM) can be used as biotic/abiotic stress attenuators in plants, thus, there are few studies that reduce the negative effects of herbicide use on plants. Therefore, this study aimed the effect of SA and ASM as attenuators of phytointoxication caused by chlorimuron-ethyl in soybean crop. The experiment was carried out in a greenhouse in a randomized complete block design with five replicates. The treatments were not studied V3-V4 in the soybean crop were: control (without herbicide, without SA and ASM); SA (1.0 mM); ASM (1.0 mM); SA 7 days before application of the herbicide + chlorimuron-ethyl (1.0 mM + 100 g ha-1 respectively); ASM 7 days before application of the herbicide + chlorimuron-ethyl (1.0 mM + 100 g ha-1, respectively); chlorimuron-ethyl (100 g ha-1); SA in syrup mixture + chlorimuron-ethyl (1.0 mM + 100 g ha-1, respectively) and ASM in syrup mixture + chlorimuron-ethyl (1.0 mM + 100 g ha-1, respectively). After three hours of application and at 3, 7, 14, 21 and 28 days after application (DAA), electron transport rate (ETR) evaluations were performed. On the same days the symptoms of phytointoxication and the estimation of leaf area (LA) were evaluated. At 28 DAA, plant height and dry shoot biomass were measured. The application of SA and ASM, before or in combination with chlorimuron-ethyl herbicide, reduced phytointoxication symptoms, maintained ETR indexes close to the control treatment, and minimized the reductions of LA, height and dry biomass. The application of SA and ASM attenuated the stress caused by chlorimuron-ethyl in soybean crop.

https://doi.org/10.19084/rca.16031 607 downloads

14.

Óleos essenciais no controle de Botrytis cinerea: influência na qualidade pós-colheita de uvas ‘Rubi’
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Garcia, Carla

; Rodrigues, João Domingos

; Mazaro, Sergio Miguel

; Botelho, Renato Vasconcelos

; Faria, Cacilda Márcia Duarte Rios

Brazilian Journal of Food Technology

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2019, Volume 22 elocation e2018177

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Para que ocorra o controle de doenças na pós-colheita de uvas, são utilizados agroquímicos que, apesar de eficientes, podem causar problemas à saúde humana. Neste sentido, o presente trabalho tem o objetivo de verificar o potencial dos compostos voláteis presentes no óleo essencial de pitanga (OEP), guaçatonga (OEG) e melaleuca (OEM) no controle de Botrytis cinerea tanto in vitro quanto inoculados nas bagas de uvas ‘Rubi’, bem como verificar a indução de resistência nesses frutos. Dessa forma, avaliou-se o crescimento micelial do B. cinerea, o controle do mofo cinzento nas uvas ‘Rubi’, a qualidade pós-colheita dessas bagas e a atividade das enzimas polifenoloxidase (PPO), fenilalanina amônio liase (PAL), glucanase (GLU) e quitinase. De acordo com os resultados, observou-se que os compostos voláteis liberados pelos óleos essenciais reduziram os índices de velocidade de crescimento micelial de B. cinerea e da doença do mofo cinzento. Os tratamentos com voláteis de OEP e OEG ativaram mecanismos de indução de resistência desses frutos. Dessa forma, ressalta-se que OEP e OEG liberam compostos que apresentam a capacidade de agir diretamente sobre B. cinerea e também induzem a resistência de uvas ‘Rubi’, reduzindo o mofo cinzento nessas uvas.

Abstract Agrochemicals are used to control diseases in post-harvest grapes, but although efficient, they may cause human health problems. Thus, the objective of the present work was to verify the potential of the volatile compounds present in the essential oils of the fruits pitanga (EOP), guaçatonga (EOG) and melaleuca (EOM) in the control of Botrytis cinerea both in vitro and inoculated in ' Rubi ' grapes, as well as to verify the induction of resistance in these fruits. Thus, B. cinerea mycelial growth, grey mould control in 'Rubi' grapes, post-harvest quality of the grapes and the activities of the enzymes polyphenoloxidase (PPO), phenylalanine ammonium lyase (PAL), glucanase (GLU) and chitinase, were evaluated. According to the results it was observed that the volatile compounds released by the essential oils reduced the rates of mycelial growth of B. cinerea and of the grey mould disease, and the treatments with the volatiles from EOP and EOG activated the resistance induction mechanisms of these fruits. Thus, EOP and OEG release compounds that can act directly on B. cinerea and also induce resistance in the 'Rubi' grapes, reducing the grey mould in these grapes.

https://doi.org/10.1590/1981-6723.17718 857 downloads

15.

Muscle Strength Training is Better than the Use of Growth Hormone (GH) in Bone Health of Wistar Rats
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Caetano, Heliard Rodrigues dos Santos

; Castoldi, Robson Chacon

; Zanuto, Éverton Alex Carvalho

; Ozaki, Guilherme Akio Tamura

; Garcia, Thiago Alves

; Pereira, João Domingos Augusto dos Santos

; Constantino, Carlos José Leopoldo

; Bremer-Neto, Hermann

; Giometti, Ines Cristina

; Camargo, Regina Celi Trindade

; Camargo-Filho, José Carlos Silva

; Belangero, William Dias

International Journal of Morphology

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2019, Volume 37 N. 1 Pages 104 - 110

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

SUMMARY: The objective of this study was to evaluate the effects of growth hormone (GH) and muscle strength training (ST) on the composition of bone tissue of Wistar rats through Raman spectroscopy. In total, 40 male rats were randomly distributed into four groups: (N = 10) control (C), control with the application of GH (GHC), strength training (T), and strength training with the application of GH (GHT). The training consisted of four series of 10 water jumps, performed three times a week, with an overload corresponding to 50 % of body weight and duration of four weeks. GH was applied at a dose of 0.2 IU / kg in each animal three times a week and every other day. After four weeks, the animals were euthanized and the right femurs collected for analysis of the bone structure. Raman spectroscopy (ER) was used to observe the following compounds from their respective bands: Calcium Carbonate-Triglycerides (fatty acids) 1073 cm-1, Collagen type I 509 cm-1, Bone-DNA Phosphate (Protein) 589 cm-1, Phosphate Phospholipids 1078 cm-1. For the statistical analysis, the Shapiro-Wilk and ANOVA One-Way variance analysis normality tests were performed, followed by the Tukey post-test. The results showed an increase in the concentrations of calcium carbonate-triglycerides (fatty acids), type I collagen, bone phosphate-DNA (protein), and phosphate phospholipids in all experimental groups, with or without ST and/or GH , But only the isolated training group differed significantly from the control group (P <0.05). It was concluded that all treatments could promote bone tissue gain, however, only the T group demonstrated a significant difference in the mineral compounds analyzed.

RESUMEN: El objetivo del estudio fue avaluar el efecto de la aplicación de la hormona del crecimiento (GH) y entrenamiento de la fuerza muscular (EF) en la composición del tejido óseo de ratas Wistar a partir de la espectroscopía Raman. Fueron utilizadas 40 ratas machos distribuidas de forma aleatoria en cuatro grupos (n=10): control (C), control y aplicación de GH (GHC), entrenamiento de la fuerza muscular (EF) y entrenamiento de la fuerza muscular y aplicación del GH (GHE). El entrenamiento fue consistió en cuatro series de 10 saltos acuáticos, realizados tres veces en la semana, con sobrecarga correspondiente a 50 % de la masa corporal y durante cuatro semanas. El GH fue aplicado en la dosificación de 0,2 UI/kg en cada animal, tres veces en la semana y en días alternados. Después de cuatro semanas, los animales fueran eutanasiados y retirados los fémures derechos para un análisis de la estructura ósea. La espectroscopía Raman fue utilizada para observar los siguientes compuestos a partir de las respectivas bandas: Carbonato de Calcio-Triglicéridos (ácidos grasos) 1073 cm-1, Colágeno Tipo I 509 cm-1, Fosfato Óseo-DNA (Proteína) 589 cm1, Fosfato Fosfolípidos 1078 cm-1. Para el análisis estadístico, fueron realizadas las pruebas Shapiro-Wilk y el análisis de variancia ANOVA One-Way, seguida de test post hoc de Tukey. Los resultados revelaran aumento de la concentración de Carbonato de Calcio-Triglicéridos (ácidos grasos), Colágeno Tipo I, Fosfato Óseo- DNA (Proteína), Fosfato Fosfolípidos en todos los grupos experimentales, asociados o no a la realización del EF y/o aplicación del GH. Además, solamente el grupo EF mostró diferencia significativa del grupo C (p<0,05). Es posible concluir que todos los tratamientos mostraran aumentos en el tejido óseo, sin embargo, solamente el grupo T demostró una diferencia significativa en los compuestos minerales analizados.

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