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Abstract The iAtlantic Project has established an international collaborative strategy to improve mapping and characterization of deep and open ocean ecosystems in understudied regions of the Atlantic and evaluate their health. In December 2022, the first iAtlantic expedition in the South Atlantic set off to map and explore seafloor ecosystems in the Santos Basin slope (200-1,000 m depths) in collaboration with the Petrobras ‘Santos Basin - Regional Characterization Project.’ The 17-day ‘iAtlantic_BR10-Petrobras’ cruise was conducted on board the research vessel NPqHOc Vital de Oliveira (Brazilian Navy) and performed (a) water column structure characterization, (b) seafloor morphology mapping, (c) description of benthic habitats and communities by seafloor imagery and biological/ geological sampling, and (d) ex-situ experiments to assess the functioning of sedimentary ecosystems and their responses to climate-related environmental changes. This study describes the rationale behind the iAtlantic_BR10-Petrobras cruise science plan, reports its sampling strategy and methods, and summarizes its collected data and preliminary results.

https://doi.org/10.1590/2675-2824071.23069jaap

Multidisciplinary Scientific Cruises for Environmental Characterization in the Santos Basin – Methods and Sampling Design
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Moreira, Daniel L.

; Dalto, Adriana G.

; Figueiredo JR, Alberto G.

; Valerio, Aline M.

; Detoni, Amalia M. S.

; Bonecker, Ana C. T.

; Signori, Camila N.

; Namiki, Cláudia

; Sasaki, Dalton K.

; Pupo, Daniel V.

; Silva, Danilo A.

; Kutner, Deborah S.

; Duque-Castaño, Diana C.

; Marcon, Eduardo H.

; Gallotta, Fabiana D. C.

; Paula, Fabiana S.

; Gallucci, Fabiane

; Roque, Gabriela C. F.

; Campos, Giulia S.

; Fonseca, Gustavo

; Mattos, Gustavo

; Lavrado, Helena P.

; Silveira, Ilson C. A. da

; Costa, Jessica O.

; Santos Filho, João R. dos

; Carneiro, Juliane C.

; Moreira, Julio C.F.

; Rozo, Laura

; Araujo, Leandro F.M.

; Lazzari, Letícia

; Silva, Letícia O. da

; Michelazzo, Luan S.

; Fernandes, Luciano F.

; Dottori, Marcelo

; Araújo Jr., Marcus A. G. de

; Chuqui, Mateus G.

; Ceccopieri, Milena

; Borges-Silva, Milton

; Kampel, Milton

; Bergo, Natascha M.

; Silva, Paulo V. M.

; Tura, Pedro M.

; Moura, Rafael B. de

; Romano, Renato G.

; Martins, Renato P.

; Carreira, Renato S.

; Toledo, Rodrigo G.A.

; Bonecker, Sérgio L.C.

; Disaró, Sibelle T.

; Rodrigues, Silvana V.

; Corbisier, Thais N.

; Vicente, Thaisa M.

; Paiva, Vitor G. de

; Pellizari, Vivian H.

; Belo, Wellington C.

; Brandini, Frederico P.

; Sousa, Silvia H.M

Ocean and Coastal Research

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2023, Volume 71 Suppl. 3 elocation e23022

Abstract: En | Text: En | PDF: En

Abstract The Santos Basin (SB) is the main petroliferous basin in the Brazilian continental margin and one of the most studied marine areas in Brazil. However, historical data suggest that new efforts should be carried out to acquire quantitative biological data, especially in the deep sea, to establish the baseline of essential ocean variables in different ecosystems for future monitoring programs. The Brazilian energy company Petrobras planned and executed 24 oceanographic cruises over a period of 2 years to assess the benthic (SANSED cruise) and pelagic (SANAGU cruise) systems of the SB (356 days at sea in 2019 and 2021/2022). These efforts were part of the Santos Project, which comprised a comprehensive environmental study aimed at investigating benthic and pelagic variables to characterize ecology, biogeochemistry, thermohaline properties of water masses, and ocean circulation patterns, geomorphology, and sedimentology, as well as organic and inorganic chemistry. Here we present the detailed sampling designs and the field methods employed on board, during the SB scientific cruises. All sampling protocols were based on standardized approaches. For the benthos analyses, triplicate sediment samples were performed using a GOMEX-type box corer (0.25 m²) or a large modified Van Veen grab (0.75 m²) at 100 stations ranging from 25 to 2400 m depth. At each station, 25 geochemical and physico-chemical parameters were analyzed in addition to micro-, meio-, and macrofauna and living foraminifera samples. For the pelagic system, 60 stations were selected to investigate the plankton community, ranging in size from pico- to macroplankton, through vertical, horizontal, and oblique net hauls (20, 200, and 500 μm mesh size), as well as 25 biogeochemical parameters collected with an aid of a CTD-rosette sampler. Part of this scientific information also serves the Regional Environmental Characterization Project (PCR-BS) in support of Petrobras’ Santos Basin drilling licensing process led by the Brazilian Environmental Agency – IBAMA. This project contributes to the sustainable development of the SB, in line with the guidelines of the United Nations Decade of Ocean Science for Sustainable Development.

https://doi.org/10.1590/2675-2824071.22072dlm

Isso a marca não o mostra: omissões e distorções comunicacionais empresariais à luz do imaginário
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Torres, Leonardo

; Souza, Rafael Rodrigues de

Intercom: Revista Brasileira de Ciências da Comunicação

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2023, Volume 46 elocation e2023135

Abstract: En Es Pt | Text: En Es Pt | PDF: En | PDF: Es | PDF: Pt

Abstract We propose a symbolic investigation of two publications posted on Instagram by Heineken and Carrefour. This choice was made because they represent the idea of “political correctness”. The objective is to analyze these communications from an imaginary theories perspective, as suggested by M. Contrera, G. Durand, C. Jung, and others, as this action allows the identification of hidden aspects in the publications and the perception of what information is there, however, in addition to the obviousness of commercial interests that aim to reinforce the brand’s good public reputation. Our conclusions suggest that it is possible to change this biased perception by building a critical mass sufficiently qualified to recognize these mechanisms that lead to communication one-sidedness.

Resumen Se propone la investigación simbólica de dos publicaciones en Instagram por Heineken y Carrefour. Esta elección se hizo porque representan la idea de “corrección política”. El objetivo es analizar estas comunicaciones a la luz de teorías imaginarias, como lo sugieren M. Contrera, G. Durand, C. Jung y otros, ya que esta acción permite identificar aspectos ocultos en las publicaciones y la percepción de qué información hay allí, sin embargo, además de la evidencia de intereses comerciales que apuntan a reforzar la buena reputación pública de la marca. Las conclusiones sugieren que es posible cambiar esta percepción sesgada construyendo una masa crítica, suficientemente calificada para reconocer estos mecanismos que conducen a la unilateralidad de la comunicación.

Resumo Propõe-se a investigação simbólica de duas publicações postadas no Instagram pela Heineken e pelo Carrefour. Essa escolha se deu por estas representarem a ideia do “politicamente correto”. O objetivo é analisar estas comunicações à luz das teorias do imaginário, conforme sugerem M. Contrera, G. Durand, C. Jung e outros, pois tal ação permite a identificação de aspectos escamoteados nas publicações e a percepção de quais informações estão ali, porém, além das obviedades dos interesses comerciais que visam reforçar a boa reputação pública da marca. As conclusões sugerem que é possível a mudança dessa percepção enviesada a partir da construção de uma massa crítica, suficientemente capacitada para reconhecer estes mecanismos que levam a uma unilateralidade comunicacional.

https://doi.org/10.1590/1809-58442023135pt

Therapeutic ultrasound ameliorates hyperalgesia and edema on CFA-induced persistent inflammatory response in mice
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Rodrigues, Mariana

; Barbosa, Rafael I.

; Neves, Lais M.S.

; Kuriki, Heloyse U.

; Gonçalves, Elaine C.D.

; Santos, Adair R.S.

; Dutra, Rafael C.

; Marcolino, Alexandre M.

Brazilian Journal of Anesthesiology

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2023, Volume 73 N. 5 Pages 626 - 634

Abstract: En | Text: En | PDF: En

Abstract Background: The present study investigated the effects of pulsed and continuous ultrasound (USP and USC) in edema and hyperalgesia after chronic inflammatory process induced by Complete Freund’s Adjuvant-CFA and analyzing the relationship of the application frequency of ultrasound, in pro- and anti-inflammatory cytokine production. Methods: Forty-five animals were divided into 9 groups; all animals from groups 2 to 9 were subjected to a persistent inflammation model induced by CFA in mice. We report the effects and the underlying action mechanisms of USP and USC in the animals which were irradiated two, three or five times a week on the left hind paw. The analyses performed in this study were: evaluation of hind paw edema through the plethysmometer, evaluation of thermal hyperalgesia through withdrawal test using a water container at 44.5°C (± 0.5°C), and the plantar region of the left paw which was removed for analysis of cytokines. Results: Our results showed that USP and USC consistently reduced paw edema, and pulsed ultrasound showed a higher significant effect than the continuous mode. Moreover, groups with irradiation frequency of five times a week presented an inhibition of the edema, and groups with frequency of three or two times a week reduced mainly hyperalgesia, in comparison with the control group. The beneficial effects of the US then seem to be associated with upregulation of anti- and pro-inflammatory mediators, such as IL-10 and IL-6, respectively. Conclusion: This study provided evidence that ultrasound constitutes an important non-pharmacological intervention for the management of inflammatory and pain states.

https://doi.org/10.1016/j.bjane.2022.08.004

Water salinity and air temperature on quail production and organ characteristics
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Furtado, Dermeval A.

; Rodrigues, Ladyanne R.

; Rodrigues, Valéria P.

; Ribeiro, Neila L.

; Silva, Rafael C.

; Farias, Soahd A. R.

Revista Brasileira de Engenharia Agrícola e Ambiental

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apr 2022, Volume 26 N. 4 Pages 313 - 318

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O fornecimento de água salgada no semiárido é uma prática recorrente, visto que existe uma grande escassez de água para uso na alimentação animal. Assim, na maioria das vezes a água oferecida às aves pode apresentar sal acima do recomendado. O presente estudo teve como objetivo avaliar o desempenho produtivo e a morfometria dos órgãos de codornas japonesas à medida que eram abastecidas com água potável com diferentes concentrações de cloreto de sódio, sendo mantidas em conforto e sob estresse térmico. As aves receberam água com valores crescentes de condutividade elétrica (1,5, 3,0, 4,5 e 6,0 dS m-1), e foram mantidas em câmara climática em temperatura do ar termoneutra (24 °C) e sob estresse térmico (32 °C), sendo distribuídas em um delineamento inteiramente casualizado e esquema fatorial 2 × 4. As condutividades elétricas da água não afetaram o desempenho das aves, exceto para o peso da moela, que apresentou efeito linear crescente com o aumento da condutividade elétrica. Na temperatura de estresse, houve redução no consumo de ração, peso e massa do ovo e na conversão alimentar por dúzia de ovos, porém sem efeito no peso do coração, fígado e moela. Codornas japonesas na fase de produção podem consumir água com condutividade elétrica de até 6,0 dS m-1, apresentando bom desempenho produtivo e sem comprometer a morfometria dos órgãos.

ABSTRACT The supply of salt water in the semiarid region is a recurrent practice, as there is a severe shortage of water for use in animal consumption. Thus, most of the times the water offered to the birds can contain salts above the recommended amount. The present study aimed to evaluate the production performance and morphometry of the organs of Japanese quails as they were supplied with drinking water with different concentrations of sodium chloride, while being maintained in comfort and under thermal stress. The birds received water with increasing electrical conductivity (1.5, 3.0, 4.5 and 6.0 dS m-1) and were kept in a climate chamber at thermoneutral air temperature (24 °C) and under thermal stress (32 °C), being distributed in a completely randomized design and 2 × 4 factorial scheme. Water electrical conductivities did not affect the performance of the birds, except for the weight of the gizzard, which showed an increasing linear effect as the electrical conductivities increased. At the stress temperature, there was reduction in feed intake, egg weight and mass, and in feed conversion per dozen eggs, but with no effect on the weights of the heart, liver and gizzard. Japanese quails in the production phase can consume water with electrical conductivity of up to 6.0 dS m-1, showing good production performance and without compromising organ morphometry.

https://doi.org/10.1590/1807-1929/agriambi.v26n4p313-318

Antennal sensilla in Cyclocephala literata Burmeister, 1847 (Coleoptera: Scarabaeidae: Dynastinae)
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Nagamine, Rafael Reverendo Vidal Kawano

; Costa, Cleicimar Gomes

; Fuhrmann, Juares

; Rodrigues, Sérgio Roberto

Biota Neotropica

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2022, Volume 22 N. 2 elocation e20211292

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo: Adultos do besouro Cyclocephala literata Burmeister, 1847 desempenham importantes funções de polinização em plantas da família Magnoliaceae. É conhecido que muitos insetos encontram plantas hospedeiras pela detecção de voláteis pelas sensilas antenais. Cyclocephala possui os três antenômeros distais lamelados e na superfície de cada lamela possuem sensila trichodea, caética, placódea, coelocônica, basicônica e ampulacea. Foram encontrados três tipos de sensilas placódeas (tipo I, II e III), e dois tipos de sensilas coelocônicas (tipo I e II). Fêmeas apresentam em média 10.776 sensilas, das quais 10.214 são sensilas placódeas, 536 são sensilas coelocônicas e 26 são sensilas basicônicas. Os machos apresentaram em média 10.386 sensilas, das quais 9.873 são sensilas placódeas, 464 são sensilas coelocônicas e 49 são sensilas basicônicas. Machos e fêmeas apresentam quantidades semelhantes de sensilas e as sensilas placódeas são predominantes. As diferenças encontradas nas quantidades de sensilas em machos e fêmeas também foram encontradas em outros besouros e foram atribuídas a detecção de feromônios coespecíficos por um dos sexos, ou para detecção de voláteis de plantas. A sensila antenal de C. literata é descrita e quantificada no presente estudo, e algumas perspectivas sobre as diferenças entre os tipos de comunicação química, polinização e dimorfismo antenal é discutido.

Abstract: Adults of the beetle Cyclocephala literata Burmeister, 1847 are important pollinators to some Magnoliaceae. Is known that insects could find host plants by detecting volatiles through antennal sensilla. Cyclocephala has its three distal antennomeres lamellate, and the surface of each lamella has sensilla trichodea, chaetica, placodea, coeloconica, basiconica and ampullacea. Three kinds of sensilla placodea were found (type I, II and III), and two kinds of sensilla coeloconica were observed (type I and II). Females have on average 10,776 sensilla, of which 10,214 are sensilla placodea, 536 are sensilla coeloconica, and 26 are sensilla basiconica. Males have on average 10,386 sensilla, of which 9,873 are sensilla placodea, 464 are sensilla coeloconica, and 49 are sensilla basiconica. Males and females have similar quantities of sensilla, and sensilla placodea are predominant. The differences observed in the number of sensilla of males and females were found in other beetles and were attributed to the detection of cospecific sexual pheromones by one of the sexes, or to the detection of plant volatiles. The antennal sensilla of C. literata is described and quantified in present study, and some perspectives about the differences kind of chemical communication, pollination, and antennae dimorphism is discussed.

https://doi.org/10.1590/1676-0611-bn-2021-1292

Robot-assisted simple prostatectomy: the evolution of a surgical technique
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Rodrigues, Gilberto J.

; Sawczyn, Guilherme V.

; Guglielmetti, Giuliano B.

; Fazoli, Arnaldo J. C.

; Tanure, Luís H. R.

; Nahas, William C.

; Coelho, Rafael F.

International braz j urol

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jun 2021, Volume 47 N. 3 Pages 682 - 683

Abstract: En | Text: En | PDF: En

ABSTRACT Purpose: Enucleation of a large prostate is the best surgical choice for patients refractory to clinical treatment (1,2). Since the first robot-assisted simple prostatectomy (RASP) was described (3,4), some technical modifications (5–7) and different approaches to reach the adenoma have been proposed (8,9). The aim of this video is to demonstrate three different techniques of RASP. Materials and Methods: The first procedure begins with a transversal incision over the bladder neck, the second is a transvesical approach and the last one is a Retzius-sparing RASP. All techniques were performed with a vesico-urethral anastomosis. Results: Three patients underwent RASP, each one with a different approach. Patients presented mean age of 66±4.4 years, PSA baseline level of 7.8±3ng/mL, IPSS score of 17.7±4.5, maximum urine flow of 8.3±1.5mL/seg and 122.3±11.2cm3 of prostate volume. The mean operative time was 63±8 minutes, estimated blood loss of 106.7±11.5mL, prostate weight of the surgical specimen of 106.3±8 grams and 1 day of length of stay. No continuous bladder irrigation was required and there was no complication. The mean postoperative PSA and IPSS were 0.7±0.3ng/mL, 4.7±1.5. The maximum urine flow raised to 20±4.4mL/seg. Conclusions: RASP with vesico-urethral anastomosis allowed minimal blood loss, short length of stay and great functional outcomes. All the three approaches allowed to perform this technique in a safe way, while showing different alternatives to reach the adenoma.

https://doi.org/10.1590/s1677-5538.ibju.2020.0744 286 downloads

Evaluation of mesalazine polymeric conjugate in the treatment of actinic proctitis in rats
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Nunes, Vinícius Rodrigues Taranto

; Vidigal, Paula Vieira Teixeira

; Pereira, Márcio Tadeu

; Ladeira, Luís Carlos Duarte

; Caliari, Marcelo Vidigal

; Oliveira, Fabrício Marcus Silva

; Cesar, Aina Liz Alves

; Faraco, André Augusto Gomes

; Barbuto, Rafael Calvão

; Duval-Araujo, Ivana

Acta Cirúrgica Brasileira

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2021, Volume 36 N. 8 elocation e360805

Abstract: En | Text: En | PDF: En

ABSTRACT Purpose: The present study aimed at testing a new formulation of mesalazine linked to chondroitin sulfate and its components alone in the treatment of actinic proctitis in rats. Methods: Forty-seven female Wistar rats were submitted to pelvic radiation and divided into eight groups: control A, mesalazine A, chondroitin A, and conjugate A, gavage of the according substance two weeks after irradiation and sacrifice three weeks after oral treatment; control C, mesalazine C, chondroitin C, and conjugate C, sacrifice six weeks after oral treatment. The rectum was submitted to histological characterization for each of the findings: inflammatory infiltrate, epithelial degeneration, mucosal necrosis, and fibrosis. Results: The inflammatory infiltrate was more intense in chondroitin A, mesalazine A, and conjugate C. The collagen deposition was less intense in chondroitin A, and mesalazine A, and more intense in control C. Conclusions: Mesalazine and chondroitin alone were efficacious in inducing a delayed inflammatory response, hence reducing the late fibrosis. The conjugate was able to induce an ever more delayed inflammatory response.

https://doi.org/10.1590/acb360805 195 downloads

Clostridium perfringens α and β recombinant toxoids in equine immunization
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Freitas, Nayra F.Q.R.

; Barbosa, José D.

; Otaka, Denis Y.

; Ferreira, Marcos Roberto A.

; Rodrigues, Rafael R.

; Moreira Jr, Clóvis

; Conceição, Fabricio R.

; Salvarani, Felipe M.

Pesquisa Veterinária Brasileira

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oct 2020, Volume 40 N. 10 Pages 776 - 780

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: Clostridium perfringens é considerado um dos principais agentes causadores de enterocolites superagudas, geralmente fatais na espécie equina, devido à ação da toxina β, além de ser responsável por causar quadros graves de mionecrose, pela ação da toxina α. A grande importância desses agentes na equinocultura, deve-se a elevada mortalidade e a inexistência de vacinas, principal forma de prevenção, que garantam a imunização dessa espécie animal. O objetivo deste trabalho foi avaliar três diferentes concentrações (100, 200 e 400μg) dos toxóides recombinantes α e β de C. perfringens na imunização de equinos, bem como comparar com um grupo vacinado com um toxóide comercial. A vacina comercial não se mostrou capaz de estimular uma resposta imune e a vacina recombinante foi capaz de induzir resposta imune humoral satisfatória em equinos vacinados, provando ser uma alternativa profilática para infecção por C. Perfringens.

ABSTRACT: Clostridium perfringens is considered one of the main causative agents of superacute enterocolitis, usually fatal in the equine species, due to the action of the β toxin, and is responsible for causing severe myonecrosis, by the action of the α toxin. The great importance of this agent in the equine economy is due to high mortality and lack of vaccines, which are the main form of prevention, which guarantee the immunization of this animal species. The aim of this study was to evaluate three different concentrations (100, 200 and 400μg) of C. perfringens α and β recombinant toxoids in equine immunization and to compare with a group vaccinated with a commercial toxoid. The commercial vaccine was not able to stimulate an immune response and the recombinant vaccine was able to induce satisfactory humoral immune response in vaccinated horses, proving to be an alternative prophylactic for C. perfringens infection.

https://doi.org/10.1590/1678-5150-pvb-6677 559 downloads

10.

Susceptibility of soybean looper to lufenuron and spinosad
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Queiroz, Lucas Ferraz de

; Corassa, Janaina De Nadai

; Rodrigues, Sandra Maria Morais

; Pitta, Rafael Major

Arquivos do Instituto Biológico

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2020, Volume 87 elocation e0062019

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: A lagarta falsa-medideira (Chrysodeixis includens) é uma importante praga desfolhadora em culturas como soja e algodão no Brasil e seu principal método de controle é o uso de inseticidas químicos. Considerando a importância do controle químico para essa praga, o monitoramento da suscetibilidade de populações de C. includens é estratégico para um eficiente programa de Manejo da Resistência de Insetos. Portanto, objetivou-se com este estudo avaliar os níveis de suscetibilidade de populações de C. includens no estado de Mato Grosso, Brasil, aos inseticidas lufenurom e espinosade. Sete populações foram coletadas em cultivos de soja ao longo do estado. Para os bioensaios, lagartas em terceiro ínstar foram expostas aos inseticidas utilizando o método de contaminação superficial de dieta. Embora os compostos apresentem modos de ação distintos, a população de Tangará da Serra obteve as maiores razões de resistência para lufenurom (11,62) e espinosade (7,84) em relação à população de laboratório (referência de suscetibilidade). Apesar dos níveis de resistência terem sido baixos, é necessário manter a manutenção do monitoramento dos níveis de suscetibilidade aos inseticidas avaliados, assim como ampliar a gama de moléculas monitoradas.

ABSTRACT: The soybean looper (Chrysodeixis includens) is an important defoliation pest in crops such as soybean and cotton in Brazil. Its main control tactic is chemical insecticides. Considering the importance of chemical control for this pest, monitoring the susceptibility of C. includens populations is strategic for an efficient Insect Resistance Management. Therefore, the objective of this study was to evaluate the susceptibility levels of C. includens populations in the state of Mato Grosso - Brazil to lufenuron and spinosad. Seven populations were collected in soybean fields around the state. For the bioassays, early L3 larvae were exposed to insecticides using the diet-overlay method. Although the compounds have distinct modes of action, Tangará da Serra population had the highest resistance ratios for lufenuron (11.62) and spinosad (7.84), compared to laboratory population (susceptibility reference). Even with low resistance levels, it is necessary to maintain regional monitoring of C. includens susceptibility to the evaluated insecticides, as well as to extend the range of molecules monitored.

https://doi.org/10.1590/1808-1657000062019 1211 downloads

11.

Approach to Endoscopic Procedures: A Routine Protocol from a Quaternary University Referral Center Exclusively for Coronavirus Disease 2019 Patients
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Franzini, Tomazo Antonio Prince

; Kotinda, Ana Paula Samy Tanaka

; Moura, Diogo Turiani Hourneaux de

; Badana, Márcia Lopes Vicente

; Medeiros, Marion Sielfeld de

; Lima, Patrícia Goulart Rodrigues

; Mello, Brigitte Feiner de

; Kayano, Rafael Priante

; Carmona, Maria José Carvalho

; Rocha, Marcelo Cristiano

; Campos, Aléia Faustina

; McCarty, Thomas R.

; Guimarães, Thaís

; Moura, Maria Luisa do Nascimento

; Thompson, Christopher C.

; Moura, Eduardo Guimarães Hourneaux de

Clinics

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2020, Volume 75 elocation e1989

Abstract: En | Text: En | PDF: En

OBJECTIVES: The present coronavirus disease (COVID-19) pandemic has ushered in an unprecedented era of quality control that has necessitated advanced safety precautions and the need to ensure the adequate protection of healthcare professionals (HCPs). Endoscopy units, endoscopists, and other HCP may be at a significant risk for transmission of the virus. Given the immense burden on the healthcare system and surge in the number of patients with COVID-19, well-designed protocols and recommendations are needed. We aimed to systematically characterize our approach to endoscopic procedures in a quaternary university hospital setting and provide summary protocol recommendations. METHOD: This descriptive study details a COVID-19-specific protocol designed to minimize infection risks to patients and healthcare workers in the endoscopy unit. RESULTS: Our institution, located in São Paulo, Brazil, includes a 900-bed hospital, with a 200-bed-specific intensive care unit exclusively designed for patients with moderate and severe COVID-19. We highlighted recommendations for infection prevention and control during endoscopic procedures, including appropriate triage and screening, outpatient management and procedural recommendations, role and usage of personal protective equipment (PPE), and role and procedural logistics involving COVID-19-positive patients. We also detailed hospital protocols for reprocessing endoscopes and cleaning rooms and also provided recommendations to minimize severe acute respiratory syndrome coronavirus 2 transmission. CONCLUSION: This COVID-19-specific administrative and clinical protocol can be replicated or adapted in multiple institutions and endoscopy units worldwide. Furthermore, the recommendations and summary protocol may improve patient and HCP safety in these trying times.

https://doi.org/10.6061/clinics/2020/e1989 1053 downloads

12.

STABILIZATION STUDY OF TETRAMERIC Kluyveromyces lactis β-GALACTOSIDASE BY IMMOBILIZATION ON IMMOBEAD: THERMAL, PHYSICO-CHEMICAL, TEXTURAL AND CATALYTIC PROPERTIES
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Gennari, Adriano

; Mobayed, Francielle Herrmann

; Rafael, Ruan da Silva

; Catto, André Luis

; Benvenutti, Edilson Valmir

; Rodrigues, Rafael C.

; Sperotto, Raul Antonio

; Volpato, Giandra

; Souza, Claucia Fernanda Volken de

Brazilian Journal of Chemical Engineering

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dec 2019, Volume 36 N. 4 Pages 1403 - 1417

Abstract: En | Text: En | PDF: En

Abstract We investigated the immobilization of a tetrameric Kluyveromyces lactis β-galactosidase (EC: 3.2.1.23) (KL-Gal) on Immobead 150 using different support modification strategies. Immobead support was modified using an acid solution of H2SO4:HNO3 (3:1) (Immobead-Ac) or 5 % (v/v) glutaraldehyde (Immobead-Glu). Its unmodified form (Immobead) was also tested. Immobilization yields and efficiencies were evaluated by testing protein loads from 10 to 200 mg.g-1 support. The thermal, physico-chemical, textural and catalytic properties of the supports (modified and unmodified) and their derivatives (Immobead-KL-Gal, Immobead-Ac-KL-Gal and Immobead-Glu-KL-Gal) were analyzed. The highest immobilization yields and efficiencies were achieved with a protein load of 100 mg.g-1 support. Surface and pore areas of the Immobead support were greatly decreased after modification. Michaelis constant of the immobilized β-galactosidase increased in the derivatives. Maximum velocity decreased approximately 2.8 times for Immobead-KL-Gal and Immobead-Glu-KL-Gal, and approximately 1.4 times for Immobead-Ac-KL-Gal. In batch processes, the three derivatives could be reused successfully at least 15 times, maintaining high residual enzymatic activity during the lactose hydrolysis (in both cheese whey and milk). The tetrameric K. lactis β-galactosidase immobilized on Immobead supports via the tested treatments was stabilized and is an alternative tool for lactose hydrolysis in the dairy industry.

https://doi.org/10.1590/0104-6632.20190364s20190235 910 downloads

13.

Personality Inventory for DSM-5 (PID-5): cross-cultural adaptation and content validity in the Brazilian context
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Barchi-Ferreira, Ana Maria

; Loureiro, Sonia Regina

; Torres, Albina Rodrigues

; da Silva, Thiago Dornela Apolinário

; Moreno, André Luiz

; DeSousa, Diogo Araújo

; Chagas, Marcos Hortes Nisihara

; dos Santos, Rafael Guimarães

; Machado-de-Souza, João Paulo

; Chagas, Natália Mota de Sousa

; Hallak, Jaime Eduardo C.

; Crippa, José Alexandre de Souza

; Osório, Flávia L.

Trends in Psychiatry and Psychotherapy

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sep 2019, Volume 41 N. 3 Pages 297 - 300

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Objetivo Apresentar o processo de adaptação transcultural do Personality Inventory for DSM-5 (PID-5) para o contexto brasileiro. Métodos A adaptação transcultural envolveu as etapas de tradução independente, versão síntese e retrotradução. A validade de conteúdo foi realizada por um comitê multidisciplinar de especialistas, com avaliação quantitativa dos índices de concordância. Por fim, o pré-teste foi conduzido com a população-alvo. Resultados Todos os estágios da adaptação transcultural foram seguidos, e na maioria das estruturas avaliadas, os valores de concordância foram satisfatórios (≥ 4.75). Grande parte das sugestões de modificações feitas pelos especialistas foram acatadas, sendo as principais relacionadas a ajustes no tempo e concordância verbal e a inclusão de letras e palavras para permitir a flexão de gênero. No pré-teste nenhuma sugestão foi apresentada e o instrumento foi considerado compreensível. Conclusão A versão brasileira do PID-5 mostrou-se adequada ao contexto brasileiro sob as perspectivas semântica, idiomática, cultural e conceitual. A versão brasileira avaliada é de uso livre, foi aprovada pelas editoras responsáveis pelos direitos autorais do instrumento e é considerada oficial. Novos estudos estão sendo conduzidos para aprimorar a busca por evidencias de validade e confiabilidade.

Abstract Objective To describe the process of cross-cultural adaptation of the Personality Inventory for DSM-5 (PID-5) to the Brazilian context. Methods Cross-cultural adaptation involved the steps of independent translation of the instrument, synthesis version, and back-translation. Analysis of content validity was conducted by a multidisciplinary expert committee and consisted of quantitative assessment of agreement indicators. The test was then applied to a target population. Results All the steps required for a cross-cultural adaptation were followed and satisfactory agreement values (≥ 4.75) were reached for most of the structures assessed. Most of the changes suggested by the experts were followed; these changes consisted primarily of adjustments to verb tense and agreement and the inclusion of letters and words to allow gender inflection. In the pre-test, no suggestions were made and the instrument was considered comprehensible. Conclusion The Brazilian version of the PID-5 was found to be adequate to the Brazilian context from semantic, idiomatic, cultural, and conceptual perspectives. The Brazilian version assessed here can be freely used, was approved by the publishers who hold the copyright on the instrument, and is considered the official version of the instrument. New studies are underway to determine the validity and reliability of the PID-5.

https://doi.org/10.1590/2237-6089-2018-0098 2468 downloads

14.

ULTRASOUND-ASSISTED TRANSESTERIFICATION OF SOYBEAN OIL USING COMBI-LIPASE BIOCATALYSTS
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Freitas, Vitória O. de

; Matte, Carla R.

; Poppe, Jakeline K.

; Rodrigues, Rafael C.

; Ayub, Marco A. Z.

Brazilian Journal of Chemical Engineering

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jun 2019, Volume 36 N. 2 Pages 995 - 1005

Abstract: En | Text: En | PDF: En

Abstract We applied ultrasonic technology for the transesterification of soybean oil catalyzed by a mixture of lipases (combi-lipase: 75 % Novozym 435; 10 % Lipozyme TL-IM; and 15 % Lipozyme RM-IM), verifying the effects of ultrasonic amplitude, pulse conditions, the ethanol:oil molar ratio, enzyme concentration, and the influence of solvent (tert-butanol on the reaction. We also compared this system against ultrasound combined with mechanical stirring, and the efficiency of the combi-lipase, compared with the individual use of each lipase. The optimum conditions for the transesterification reaction were determined as enzyme concentration of 15 % (in relation to oil mass); ethanol:oil molar ratio of 3:1; ultrasonic amplitude of 30 %, duty cycle of 50 % and time pulse of 15 sec. The yields of conversion of ethyl esters with and without solvent were similar, indicating that the use of solvents during enzymatic transesterification reactions is not necessary when ultrasonic technology is applied to the system. The combination of mechanical stirring and ultrasound did not improve the yields of conversion compared to ultrasonic technology alone. The proposed combi-lipase produced higher yields of ethyl esters (75 %) than the individual lipases (55 %) in 5 h under ultrasonic-assisted batch reactions.

https://doi.org/10.1590/0104-6632.20190362s20180455 946 downloads

15.

One-Pot Synthesis of NEMP, a VX Surrogate, and Reactivation of NEMP-Inhibited Electrophorus Eel Acetylcholinesterase by Current Antidotes
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Cavalcante, Samir F. A.

; Kitagawa, Daniel A. S.

; Rodrigues, Rafael B.

; Silva, Taynara C.

; Bernardo, Leandro B.

; Correa, Ana Beatriz A.

; Simas, Alessandro B. C.

Journal of the Brazilian Chemical Society

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may 2019, Volume 30 N. 5 Pages 1095 - 1102

Abstract: En | Text: En | PDF: En

The use of nerve agents, organophosphorus compounds which irreversibly inhibit acetylcholinesterase, in conflicts and terrorism has gained renewed geopolitical status in recent years. As no “universal antidote” has ever been produced, the development of antidotes to reactivate enzyme is clearly warranted. Given the fact that nerve agents are restricted substances, the use of surrogates allows non-qualified laboratories to develop research on novel antidotes. We herein present an alternative and efficient synthesis of NEMP, O-(4-nitrophenyl) O-ethyl methylphosphonate, a venomous agent X (VX) surrogate, and Ellman’s test procedures for inhibition and reactivation of Electrophorus eel acetylcholinesterase involving the NEMP-generated adduct.

https://doi.org/10.21577/0103-5053.20180246 734 downloads

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