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ABSTRACT Neospora caninum is an important worldwide parasite responsible for causing abortion in animals. Due to limited information on the occurrence of infection by this parasite in the state of Rondônia, Brazil, this study aimed to determine the seroprevalence and identify the risk factors associated with the infection in slaughtered cattle, from 19 municipalities distributed in seven microregions of the state. A total of 494 samples were obtained and subjected to anti-N. caninum antibodies, using the Indirect Immunofluorescence Reaction technique. Antibodies were detected in 5.06% (25/494) of the samples, in 30.30% (10/33) of farms, in nine municipalities located in four microregions of Rondônia. Of all the animals analyzed, 4.81% of the females (20/416) and 6.41% of the males (05/78) were seropositive for the parasite, with “abortion in the last 12 months” being considered an important risk factor for the occurrence of infection (OR = 9.54; p = 0.01). The present study points out the prevalence of anti-N. caninum antibodies in 5.06% of slaughtered animals and abortion as the main risk factor associated with infection by N. caninum, thus contributing to the elucidation of the epidemiology of this protozoan in Rondônia, Brazil.

RESUMO Neospora caninum é um importante parasito, responsável por causar aborto em animais e distribuído mundialmente. Devido às informações limitadas sobre a ocorrência da infecção por esse parasito no estado de Rondônia, Brasil, este estudo teve como objetivo determinar a soroprevalência e identificar os fatores de risco associados à infecção em bovinos abatidos, oriundos de 19 municípios, distribuídos em sete microrregiões do estado. Um total de 494 amostras foi obtido e submetido à pesquisa de anticorpos anti-N. caninum, por meio da técnica de reação de imunofluorescência indireta. Os anticorpos foram detectados em 5,06% (25/494) das amostras, em 30,30% (10/33) das propriedades rurais, de nove municípios, localizados em quatro microrregiões de Rondônia. De todos os animais analisados, 4,81% das fêmeas (20/416) e 6,41% dos machos (05/78) foram soropositivos para o parasito, sendo o “aborto nos últimos 12 meses” considerado um importante fator de risco para a ocorrência da infecção (OR = 9,54; P = 0,01). O presente estudo aponta a prevalência de anticorpos anti-N. caninum em 5,06% dos animais abatidos e o aborto como o principal fator de risco associado à infecção por N. caninum, contribuindo assim para a elucidação da epidemiologia desse protozoário em Rondônia, Brasil.

https://doi.org/10.1590/1678-4162-12887

Identifying agroecological transition pathways based on the Global Approach to Agricultural Systems: the case of family livestock farmers in northern Uruguay
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Ferreira Rivaben, I.

; Rossi, V.

; Figari, M.

; Chia, E.

Agrociencia Uruguay

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2023, Volume 27 elocation e1069

Abstract: Es En Pt | Text: Es En Pt | PDF: Es | PDF: En | PDF: Pt

Resumen: La agroecología representa una de las principales alternativas a modos de producción generados por la revolución verde, tanto en Uruguay como en otros países. Aparece como una respuesta al cambio climático, a la gestión de la biodiversidad y a la restauración de los recursos naturales; y más recientemente como respuesta a la crisis de la pandemia COVID-19. En Uruguay la agroecología, a pesar de que existe una Ley de Plan Nacional para el Fomento de la Producción con Bases Agroecológicas desde 2018, es marginal y la investigación sobre procesos de transición agroecológica es muy incipiente. El objetivo de este trabajo es identificar prácticas agroecológicas y posibles caminos de transición hacia la agroecología de los ganaderos familiares del norte uruguayo utilizando una metodología que se centra en el estudio de las prácticas técnicas, sociales y organizativas, lo cual permite acceder al esquema de funcionamiento y comprender los procesos de toma de decisiones en los sistemas de producción de tipo familiar. Las prácticas socio-productivas evidenciadas en la investigación permitieron elaborar una tipología que representa posibles caminos por los cuales estos ganaderos transitan hacia una producción y gestión agroecológica de las explotaciones. Los resultados de este estudio pueden ser utilizados para fortalecer procesos de concepción o de co-concepción, a través de la selección y difusión de innovaciones con acciones y políticas públicas más cercanas a los productores.

Abstract: Agroecology represents one of the main alternatives to production models generated by the green revolution, both in Uruguay and other countries. It appears as a response to climate change, biodiversity management, nature resource restoration, and, more recently, as a response to the COVID-19 pandemic crisis. In Uruguay, agroecology is marginal despite the existence of a law for a National Plan to Encourage Production with Agroecological Bases since 2018. Moreover, research on agroecological transition processes is very incipient. This study aims to identify agroecological practices and possible transition paths towards agroecology of family livestock farmers in northern Uruguay. The methodology used focuses the study on technical, social and organizational practices, allowing access to the operation scheme and understanding the decision-making processes in family-type production systems. The socio-productive practices revealed in the research allowed the elaboration of a typology that represents possible ways for these livestock farmers to move towards agroecological production and management of their farms. The results of this study can be used to strengthen processes of conception or co-conception by selecting and disseminating innovation with actions and public policies closer to farmers.

Resumo: A agroecologia representa uma das principais alternativas aos modos de produção gerados pela revolução verde, tanto no Uruguai como em outros países. Aparece como uma resposta às alterações climáticas, à gestão da biodiversidade e à restauração dos recursos naturais; e mais recentemente como uma resposta à crise pandêmica da COVID-19. No Uruguai, a agroecologia é marginal, apesar da existência de uma Lei do Plano Nacional para a Promoção da Produção de Base Agroecológica desde 2018, e a investigação sobre processos de transição agroecológica é muito incipiente. O objetivo deste trabalho é identificar práticas agroecológicas e possíveis vias de transição para a agroecologia dos criadores de gado familiares no norte do Uruguai, utilizando uma metodologia centrada no estudo das práticas técnicas, sociais e organizacionais, que permite o acesso ao esquema de funcionamento e a compreensão dos processos de tomada de decisão em sistemas de produção de tipo familiar. As práticas socio-produtivas reveladas pela investigação permitiram elaborar uma tipologia que representa as possíveis formas de evolução destes criadores de gado para a produção e gestão agro-ecológica das suas explorações. Os resultados deste estudo podem ser utilizados para reforçar os processos de concepção ou co-concepção, através da seleção e divulgação de inovações com ações e políticas públicas mais próximas dos produtores.

https://doi.org/10.31285/agro.27.1069

Updated Anopheles mosquitos abundance and distribution in north-eastern malaria-free area of Argentina
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STEIN, MARINA

; MARTIN, MÍA E.

; RAMÍREZ, PATRICIA G.

; ETCHEPARE, EDUARDO G.

; ORIA, GRISELDA I.

; ROSSI, GUSTAVO C.

; KURUC, JORGE

; ESTALLO, ELIZABET L.

Anais da Academia Brasileira de Ciências

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2023, Volume 95 Suppl. 2 elocation e20220956

Abstract: En | Text: En | PDF: En

Abstract Malaria is the most important parasitic disease worldwide. In 2019, more than 679,441 cases of malaria were reported in the American region. During this study, Argentina was in malaria pre-elimination autochthonous transmission phase with the aim of being declared as malaria-free country. The aim of this work was to assess the influence of remote sensing spectral indices (NDVI, NDWI) and climatic variables (temperature, relative humidity and precipitation) on the distribution and abundance of Anopheles mosquitoes, in four localities with different degrees of anthropogenic disturbance and with previous malaria cases records located , in a historical malarious area in northeastern of Argentina. Between June 2012 and July 2014, mosquitoes were collected. We collected 535 Anopheles adult mosquitoes. Anopheles strodei s.l. was the most abundant species. The greatest richness, diversity and abundance of species were registered in wild and semi-urban environments. The abundance of Anopheles presented a negative association with relative humidity and mean temperature, but positive with mean maximum temperature. The most important variables determining Anopheles total abundance and distribution were NDWI Index and distance to vegetation. The abundance of An. strodei s.l., was positive associated with water areas whereas the NDVI Index was negatively associated.

https://doi.org/10.1590/0001-3765202320220956

Chronic medical conditions associated with invasive pneumococcal diseases in inpatients in teaching hospitals in São Paulo city: Estimating antimicrobial susceptibility and serotype-coverage of pneumococcal vaccines
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Kfouri, Renato A.

; Brandileone, Maria-Cristina C.

; Sáfadi, Marco Aurélio P.

; Richtmann, Rosana

; Gilio, Alfredo E.

; Rossi, Flavia

; Guimarães, Thais

; Bierrenbach, Ana Luiza

; Moraes, José C.

Brazilian Journal of Infectious Diseases

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2023, Volume 27 N. 2 elocation 102746

Abstract: En | Text: En | PDF: En

ABSTRACT Background: Chronic conditions increase the risk of invasive pneumococcal diseases (IPD). Pneumococcal vaccination remarkably reduced IPD morbimortality in vulnerable populations. In Brazil, pneumococcal vaccines are included in the National Immunization Program (PNI): PCV10 for < 2 years-old, and PPV23 for high risk-patients aged ≥ 2 years and institutionalized ≥ 60 years. PCV13 is available in private clinics and recommended in the PNI for individuals with certain underlying conditions. Methods: A retrospective study was performed using clinical data from all inpatients from five hospitals with IPD from 2016 to 2018 and the corresponding data on serotype and antimicrobial-non-susceptibility of pneumococcus. Vaccine-serotype-coverage was estimated. Patients were classified according to presence of comorbidities: healthy, without comorbidities; at-risk, included immunocompetent persons with specific medical conditions; high-risk, with immunocompromising conditions and others Results: 406 IPD cases were evaluated. Among 324 cases with information on medical conditions, children < 5 years were mostly healthy (55.9%), while presence of comorbidity prevailed in adults ≥ 18 years old (> 82.0%). Presence of ≥1 risk condition was reported in ≥ 34.8% of adults. High-risk conditions were more frequent than at-risk in all age groups. Among high-risk comorbidity (n = 211), cancer (28%), HIV/AIDS (25.7%) and hematological diseases (24.5%) were the most frequent. Among at-risk conditions (n = 89), asthma (16.5%) and diabetes (8.1%) were the most frequent. Among 404 isolates, 42.9% belonged to five serotypes: 19A (14.1%), 3 (8.7%), 6C (7.7%), 4 and 8 (6.2% each); 19A and 6C expressed antimicrobial-non-susceptibility. The vaccine-serotype-coverage was: PCV10, 19.1%, PCV13, 43.8%; PCV15, 47.8%; PCV20, 62.9%; PCV21, 65.8%, and PPV23, 67.3%. Information on hospital outcome was available for 283 patients, of which 28.6% died. Mortality was 54.2% for those with meningitis. Conclusion: Vaccine with expanded valence of serotypes is necessary to offer broad prevention to IPD. The present data contribute to pneumococcal vaccination public health policies for vulnerable patients, mainly those with comorbidity and the elderly.

https://doi.org/10.1016/j.bjid.2023.102746

Productive performance, breast growth and digestive system development in European quail subjected to post-hatch fasting for different periods
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CRUZ, FLAVIA K.

; FIGUEROA, CHRISTIAN DOUGLAS NICHELE

; ANDRADE, KASSIANA G.

; KANEKO, ISABELLE NAEMI

; BONAGURIO, LUCAS P.

; BEZERRA JÚNIOR, JAILTON S.

; ROSSI, ROBSON M.

; MURAKAMI, ALICE E.

; SANTOS, TATIANA C.

Anais da Academia Brasileira de Ciências

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2023, Volume 95 N. 4 elocation e20191188

Abstract: En | Text: En | PDF: En

Abstract This study assessed the effect of different periods of post-hatch fasting on animal performance and breast and digestive system growth in European quail. Quail chicks were distributed in a completely randomized design, with four fasting periods (0, 24, 36, and 48 hs) and four replications of 40 birds per treatment. In 1 to 14-day-old chicks, weight gain decreased with increasing fasting time. Compensatory gain was observed from 15 days of age onward. Fasted quail had a lower length and relative weight of the digestive system than fed animals for up to 14 days. Histologically, the duodenal villus height was significantly lower in 3-day-old quail fasted for 36 hs than in those fasted for 48 hs, but this effect was not observed at 7 days. Scanning electron microscopy showed no differences in the small intestinal mucosa between fasted and fed birds at 3 days of age. Post-hatch fasting reduced the relative weight of the breast in quail aged 1 to 14 days but did not affect type IIa and IIb fiber diameter at 35 days. On the basis of these results, it is recommended that European quail raised for meat should not be fasted for more than 48 hs post-hatch.

https://doi.org/10.1590/0001-3765202320191188

Diversity and genetic structure of natural populations of the palm tree Euterpe precatoria (Arecaceae)
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Santos-Cochev-da-Cruz, Jakeline

; de-Freitas-Encinas-Dardengo, Juliana

; Souza-Rodrigues, Alex

; Vicente-Tiago, Auana

; Moreno-de-Pedri, Eliane-Cristina

; Müller-Zortéa, Kelli-Évelin

; Gonçalves-Garcia, Magali

; Alves-da-Silva-Neves, Sandra-Mara

; Bandini-Rossi, Ana-Aparecida

Revista de Biología Tropical

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dec 2022, Volume 70 N. 1 Pages 213 - 221

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen Introducción: Los ecosistemas naturales del norte de Mato Grosso, Brasil, están en proceso de fragmentación, principalmente debido al crecimiento de la población y la expansión de la agricultura. Esto pone en peligro la palma Euterpe precatoria (localmente conocida como açaí), utilizada para la construcción, extracción de palmito, preparación de jugos y helados. Objetivo: Evaluar la diversidad local y estructura genética en poblaciones nativas de E. precatoria. Métodos: Recolectamos hojas de 106 palmas fructíferas de cinco poblaciones en el estado de Mato Grosso, para análisis de marcadores microsatélites con el método de Reacción en Cadena de la Polimerasa (PCR). Resultados: Los cinco loci SSR revelaron un total de 30 alelos, que van desde 5 (EE23 y EE43) hasta 7 (EE2 y EE15), con un promedio de 6 alelos por locus. El PIC medio fue de 0.74 y confirmó baja heterocigosidad y endogamia en las poblaciones. El dendrograma UPGMA produjo dos grupos y la varianza molecular reveló una mayor diferenciación genética dentro de las poblaciones. Los loci de microsatélites presentaron un alto nivel de homocigotos lo que indica una baja diversidad genética. Conclusiones: Estas poblaciones tienen baja diversidad genética, alto promedio de alelos por locus y alelos raros y únicos. Recomendamos el establecimiento de unidades de conservación permanentes con corredores entre ellas.

Abstract Introduction: The natural ecosystems of northern Mato Grosso, Brazil, are in process of fragmentation, mainly due to population growth and the expansion of agriculture. This endangers the palm Euterpe precatoria (locally known as açaí), used for construction, palm hearts, juices and ice cream. Objective: To evaluate the local diversity and genetic structure in native populations of E. precatoria. Methods: We collected leaves from 106 fruiting palms from five populations in Mato Grosso State, for analysis of microsatellite markers with Polymerase Chain Reaction. Results: The five SSR loci revealed a total of 30 alleles, ranging from 5 (EE23 and EE43) to 7 (EE2 and EE15), with an average of 6 alleles per locus. The mean PIC was 0.74 and confirmed low heterozygosity and inbreeding. The UPGMA dendrogram produced two groups and molecular variance revealed greater genetic differentiation within populations. The high levels of homozygous microsatellite loci indicate low genetic diversity. Conclusions: These populations have low gene diversity, high average number of alleles per locus, and rare and exclusive alleles. We recommend the establishment of permanent conservation units with corridors among them.

https://doi.org/10.15517/rev.biol.trop..v70i1.42942

Años requeridos para la evaluación a campo de cultivares comerciales de trigo pan en Argentina
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Abbate, P E.

; Rossi, E. A.

; Bonamico, N. C.

; Balzarini, M. G.

; Mójica, C. J.

Agriscientia

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dec 2022, Volume 39 N. 2 Pages 3 - 3

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

RESUMEN En Argentina, la normativa vigente establece cuatro años de evaluación de rendimiento para cualquier nuevo cultivar comercial de trigo pan. El objetivo de este trabajo fue determinar la cantidad óptima de años de evaluación en ensayos comparativos de rendimiento de cultivares de trigo pan, condicionando dicho óptimo al grupo de calidad, ciclo de crecimiento y manejo fitosanitario. Se estimaron componentes de varianza (REML) con los datos de la Red de evaluación de cultivares de trigo pan de Argentina (RET), correspondientes a diez sitios experimentales. Se consideraron los tres grupos de calidad (GC1, GC2 y GC3), la primera y tercera época de siembra (E1 y E3), y manejo con y sin fungicida (CF y SF). Los resultados muestran que se requieren tres años de ensayos para evaluar el rendimiento de los diferentes grupos de calidad y ciclos de crecimiento manejados CF. Para el SF, se requieren cuatro años, excepto para GC1 en E3 y GC2 en E1, que demandan tres y más de cinco años, respectivamente. Estos resultados indican que la norma actual es apropiada para la mayoría de los casos; incluso, podría reducirse a tres CF, para mejorar la calidad de los ensayos y aumentar el número de repeticiones.

SUMMARY In Argentina, current regulations establish four years of yield evaluation for any new commercial bread wheat cultivar. The objective of this work was to determine the optimum number of years of evaluation in comparative yield trials of bread wheat cultivars, conditioning this optimum to the quality group, growth cycle and phytosanitary management. Variance components (REML) were estimated from the data of the bread wheat cultivar evaluation network of Argentina (RET), corresponding to ten experimental sites. The three quality groups (GC1, GC2 and GC3), the first and third sowing season (E1 and E3), and management with and without fungicide (CF and SF) were considered. The results show that three years of trials are required to evaluate the performance of the different quality groups and growth cycles managed CF. For SF, four years are required, except for GC1 in E3 and GC2 in E1, which demand three and more than five years, respectively. These results indicate that the current standard is appropriate for most cases; it could even be reduced to three CF, to improve trial quality and increase the number of repetitions.

https://doi.org/10.31047/1668.298x.v39.n2.36802

GIST gástrico complicado. Una forma de presentación inusual
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Quirós, Martín H.

; Crosbie, Gonzalo G.

; Andreani, Horacio D.

; Rossi, Agustín

; Orda, Carlos. A.

; Cattaneo, María E.

Revista argentina de cirugía

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jun 2022, Volume 114 N. 2 Pages 167 - 171

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

RESUMEN Los tumores GIST son un motivo de consulta cada vez más frecuente en las entrevistas de cirugía gastroenterológica. Suelen ser derivados como hallazgos incidentales o por presentar síntomas derivados de su crecimiento. Se presenta el caso clínico de una paciente que requirió internación de urgencia por síndrome anémico agudo. Se comenta su algoritmo diagnóstico y su resolución quirúrgica. Asimismo se comentan los estándares de diagnóstico y tratamiento actuales con especial foco en la estrategia quirúrgica, la cual debe ser individualizada según cada caso.

ABSTRACT Gastrointestinal stromal tumors (GISTs) are becoming an increasingly common reason for consultation in gastroenterology surgery interviews. Patients are usually referred for surgery due to an incidental finding or symptoms associated with tumor growth. We report the case of a female patient who required urgent hospitalization due to acute anemic syndrome. The diagnostic algorithm and surgical approach are described. The current standards of diagnosis and treatment are also discussed, with special focus on the surgical strategy, which must be tailored to each case.

https://doi.org/10.25132/raac.v114.n2.1583 25 downloads

The drop plate method as an alternative for Azospirillum spp viable cell enumeration within the consensus protocol of the REDCAI network
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Salvo, Luciana P. Di

; García, Julia E.

; Puente, Mariana L.

; Amigo, Josefina

; Anríquez, Analía

; Barlocco, Claudia

; Benintende, Silvia

; Bochatay, Tatiana

; Bortolato, Marta

; Cassán, Fabricio

; Castaño, Carolina

; Catafesta, Melina

; Coniglio, Anahí

; Díaz, Marisa

; Galián, Liliana R.

; Gallace, Eugenia

; García, Patricia

; Salamone, Inés E. García de

; Landa, Marianela

; Liernur, Germán

; Maneiro, María Laura

; Massa, Rosana

; Malinverni, Julieta

; Marchessi, Nicolás

; Monteleone, Emilia

; Oviedo, Silvina

; Pobliti, Lucrecia

; Portela, Gabriela

; Radovancich, Débora

; Righes, Silvia

; Rocha, Rosina

; Cáceres, Enrique Rodríguez

; Rossi, Alejandro

; Santella, Gisela

; Tortora, María Laura

; Trejo, Nora

; Valenzuela, José A.

; Vallejo, Daniela

Revista argentina de microbiología

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jun 2022, Volume 54 N. 2 Pages 61 - 70

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen La evaluación de la calidad de los inoculantes comerciales es fundamental para garantizar una adecuada respuesta de los cultivos a la inoculación dentro de un marco de bioseguridad. En este sentido, el objetivo de este trabajo fue la estandarización y validación de la técnica de la microgota para la cuantificación de Azospirillum como metodología alternativa a la técnica de siembra en superficie, propuesta actualmente en el protocolo consenso de la Red de Calidad de Inoculantes, REDCAI. Entre 14 y 25 laboratorios, tanto privados como públicos, participaron de tres ensayos independientes. A partir de ellos se obtuvieron resultados reproducibles y robustos que permiten confirmar que ambas técnicas son equivalentes y concluir que la técnica de recuento por la microgota es una alternativa adecuada para ser incluida dentro del mencionado protocolo consenso.

Abstract Quality evaluation of commercial inoculants is essential to warrant an adequate cropresponse to inoculation within a biosecurity framework. In this sense, this work is aimed at standardizing and validating the drop plate method for the enumeration of Azospirillum viable cellsas an alternative to the spread plate technique, which is currently proposed in the consensusprotocol of the REDCAI network. Between 14 and 25 private and public laboratories partici-pated in three independent trials. We obtained consistent and robust results that allowed toconfirm that both techniques are equivalent, concluding that the drop plate method is an alternative enumeration technique that is adequate to be included in the abovementioned consensusprotocol.

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10.

Biological control: lessons learned for agroecological transition in Uruguay
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Rodríguez, A.

; Chia, E.

; Rossi, V.

Agrociencia Uruguay

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2022, Volume 26 N. nspe3 elocation e967

Abstract: Es En Pt | Text: Es En Pt | PDF: Es | PDF: En | PDF: Pt

Resumen: La agroecología propone movilizar los conocimientos científicos y prácticos, tradicionales y locales de los productores para coconcebir nuevos sistemas de producción. En este artículo presentamos los resultados de trabajos sobre el control biológico desarrollados a partir de 2003 en el norte del Uruguay. Las experiencias son realizadas por diferentes organizaciones de productores familiares y por BIO-Uruguay (Batoví Instituto Orgánico), una organización social fundada por productores familiares y pobladores rurales. Los resultados de las experiencias analizadas aquí son técnicos, metodológicos y sociales. Se trata principalmente de la lucha biológica contra plagas y enfermedades de la producción agrícola y pecuaria. Se presentan las diferentes etapas del proceso de coinnovación para la obtención de soluciones biológicas a partir del reconocimiento y el uso de hongos patogénicos nativos para controlar plagas de importancia económica. El objetivo fue restituir equilibrios biológicos, contribuir a las transiciones agroecológicas de los sistemas productivos y a implementar los aprendizajes colectivos, necesarios entre los actores para la coinnovación. Estos aprendizajes contribuyen al desarrollo de las capacidades individuales e institucionales necesarias para “fabricar la agroecología” en Uruguay.

Abstract: Agroecology proposes to mobilize the scientific and practical, traditional and local knowledge of producers to develop jointly new production systems. This article presents the results of research on biological control carried out since 2003 in the north of Uruguay. Experiences were conducted by different organizations of family farmers and by BIO-Uruguay (Batoví Instituto Orgánico), a social organization founded by family farmers and rural inhabitants. The results of the analyzed experiences are technical, methodological and social, focusing mainly on the biological fight against pests and diseases of agricultural and livestock production. The different stages of the co-innovation process are presented to obtain biological solutions from the recognition and use of native pathogenic fungi to control pests of economic importance. The objectives of this study were to restore biological balances, to contribute to the agroecological transitions of the productive systems, and to enforce the collective learning, necessary among the actors, for co-innovation. These learnings contribute to the development of the individual and institutional capacities needed to "build agroecology" in Uruguay.

Resumo: A agroecologia se propõe a mobilizar o conhecimento científico e prático, tradicional, local dos produtores para co-conceber novos sistemas de produção. Neste artigo apresentamos os resultados dos trabalhos de controle biológico desenvolvidos a partir de 2003 no Norte do Uruguai. As experiências são realizadas por diferentes organizações de produtores familiares e Batoví Instituto Orgânico (BIO-Uruguai), que é uma organização social fundada por produtores familiares e habitantes rurais. Os resultados das experiências aqui analisadas são técnicos, metodológicos e socais. Trata-se principalmente da luta biológica contra pragas e doenças da produção agrícola e pecuária. São apresentadas as diferentes etapas do processo de co-inovação para a obtenção de soluções biológicas a partir do reconhecimento e uso de fungos patogênicos nativos para controlar pragas de importância econômica. O objetivo era restaurar os equilíbrios biológicos; contribuir para as transições agroecológicas dos sistemas produtivos e implementar a aprendizagem coletiva, necessária entre os atores, para a co-inovação Essas aprendizagens contribuem para o desenvolvimento das capacidades individuais e institucionais necessárias para a “fabricar a agroecologia” no Uruguai.

https://doi.org/10.31285/agro.26.967

11.

Haemagogus leucocelaenus (Diptera: Culicidae), the potential wild vector of yellow fever in the border zone of northern Misiones, Argentina
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SICHES, Julieta A.

; BERROZPE, Pablo E.

; ROSSI, Gustavo C.

; SALOMÓN, Oscar D.

; GARCÍA, Juan J.

Revista de la Sociedad Entomológica Argentina

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dec 2021, Volume 80 N. 4 Pages 136 - 141

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Haemagogus leucocelaenus (Diptera: Culicidae) is considered the primary vector of yellow fever virus (Flaviviridae) in wild environments in South America. Previous research has defined Hg. leucocelaenus as a wild species with phytotelmata-type breeding sites. The objective of this study was to report the temporal and space occurrence of Hg. leucocelaenus at the microscale in the wild-periurban fringe through a systematic and spatially stratified sampling using 81 ovitraps between April 2019 and February 2020 in the locality of Puerto Iguazú, Argentina. Of the total ovitraps, eight were positive for Hg. leucocelaenus, six in the wild environment and two in the periurban environment. Regarding the time distribution, 98.5% of the occurrence was concentrated in November and December 2019 towards the beginning of the rainy season. The results confirm the habitat plasticity of Hg. leucocelaenus and establish the aptitude of artificial containers as a methodology in monitoring studies, since they illustrate the potential of wild populations to deposit fertile eggs in them at least up to 300 m from the wild-periurban fringe. This work is a contribution to determine the temporal and space risk of yellow fever virus transmission in the region, based on the distribution patterns of this species as a function to the ecotone associated with forest borders and climatic variables.

Resumen Haemagogus leucocelaenus (Diptera: Culicidae) se considera el principal vector del virus de la fiebre amarilla (Flaviviridae) en entornos silvestres de Sudamérica. Investigaciones anteriores han definido a Hg. leucocelaenus como una especie silvestre con sitios de reproducción natural en fitotelmata. El objetivo de este estudio fue reportar la ocurrencia espacio-temporal de Hg. leucocelaenus a microescala en la franja silvestre-periurbana a través de un muestreo sistemático y espacialmente estratificado utilizando 81 ovitrampas realizado entre abril del 2019 y febrero del 2020 en la localidad de Puerto Iguazú, Argentina. Del total de las ovitrampas colocadas, ocho fueron positivas para Hg. leucocelaenus, seis en el ambiente selvático y dos en el periurbano. En cuanto a la distribución temporal, el 98,5% de las apariciones se concentraron en noviembre y diciembre de 2019, hacia el inicio de la temporada lluviosa. Estos resultados confirman la plasticidad del hábitat de Hg. leucocelaenus y establecen la aptitud de los contenedores artificiales como metodología en los estudios de seguimiento, ya que ilustran el potencial de las poblaciones silvestres para depositar huevos fértiles en ellos, al menos hasta 300 m de la franja silvestre-periurbana. Este trabajo es una contribución al riesgo espacio-temporal de transmisión del virus de la fiebre amarilla en la región, a partir de los patrones de distribución de esta especie en función del ecotono asociado a los bordes silvestres y variables climáticas.

12.

Correlation between 99mTc-TRODAT-1 SPECT and 18F-FDOPA PET in patients with Parkinson’s disease: a pilot study
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Arena, Julieta E.

; Urrutia, Leandro

; Falasco, Germán

; Leon, Magdalena Ponce de

; Vazquez, Silvia

; Rossi, Malco

; Merello, Marcelo

Radiologia Brasileira

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aug 2021, Volume 54 N. 4 Pages 232 - 237

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Objetivo: Determinar se a 99mTc-TRODAT-1 SPECT fornece resultados comparáveis aos da 18F-FDOPA PET, ferramenta menos acessível e menos amplamente disponível, no contexto de uma clínica de distúrbios do movimento. Materiais e Métodos: Neste estudo prospectivo, oito indivíduos com diagnóstico clínico de doença de Parkinson foram selecionados aleatoriamente entre pacientes em tratamento em uma clínica de distúrbios do movimento e submetidos a 99mTc-TRODAT-1 SPECT e 18F-FDOPA PET. Os resultados foram lidos por dois observadores experientes e uma análise semiquantitativa foi realizada. Resultados: As análises visual e semiquantitativa foram concordantes para todos os estudos, mostrando que a captação do radiotraçador no estriado contralateral do lado mais afetado foi menor quando a 99mTc-TRODAT-1 SPECT foi empregada. A análise semiquantitativa demonstrou uma correlação significativa entre 18F-FDOPA PET e 99mTc-TRODAT-1 SPECT (r = 0,73; p < 0,01). Conclusão: A 99mTc-TRODAT-1 SPECT parece ser uma opção válida para o estudo da função dopaminérgica em um ambiente clínico.

Abstract Objective: To determine whether technetium-99m-labeled tropane derivative single-photon emission computed tomography (99mTc-TRODAT-1 SPECT) provides results comparable to those of the less widely available, less accessible tool fluorine-18-labeled fluorodopa positron-emission tomography (18F-FDOPA PET) in the setting of a movement disorders clinic. Materials and Methods: In this prospective pilot study, eight subjects with a clinical diagnosis of Parkinson’s disease were randomly selected from among patients under treatment at a movement disorders clinic and submitted to 99mTc-TRODAT-1 SPECT and 18F-FDOPA PET. The results were read by two experienced observers, and a semiquantitative analysis was performed. Results: The visual and semiquantitative analyses were concordant for all studies, showing that radiotracer uptake in the contralateral striatum on the most affected side was lower when 99mTc-TRODAT-1 SPECT was employed. The semiquantitative analysis demonstrated a significant correlation between 18F-FDOPA PET and 99mTc-TRODAT-1 SPECT (r = 0.73; p < 0.01). Conclusion: It appears that 99mTc-TRODAT-1 SPECT is a valid option for the study of dopaminergic function in a clinical setting.

https://doi.org/10.1590/0100-3984.2020.0087 267 downloads

13.

Índice PROFUND y mortalidad intrahospitalaria en pacientes pluripatológicos. Análisis post-hoc
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Moretti, Dino

; Buncuga, Martin G.

; Laudanno, Carlos D.

; Quiñones, Nadia D.

; Scolari Pasinato, Carlos M.

; Rossi, Francisco E.

Medicina (Buenos Aires)

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jun 2021, Volume 81 N. 3 Pages 329 - 336

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract The PROFUND index was developed and valid to predict mortality at 12 months in polypathological patients (PP). However, its potential value for predicting in-hospital mortality has not been sufficiently studied. The ability of the PROFUND index in comparison with C-reactive protein (CRP), albumin, and red blood cell distribu tion width (RDW) to predict in-hospital mortality was evaluated through the subsequent analysis of a prospective cohort of 111 multiple pathological patients admitted to the clinic medical. The mean age was 75.8 ± 9.3 years. In-hospital mortality was 17% (19 patients). The median (IQR) of the PROFUND index, albumin, CRP and ADE in the deceased and survivors was 12 (4) and 6 (7) p < 0.0001, 2.5 (0.4) and 2.6 (0.8) p 0.295, 58 (64) and 40 (60) p 0.176, 14.5 (2) and 14.6 (3) p 0.523, respectively. The multivariate logistic analysis showed that the PROFUND index is associated with in-hospital mortality (p 0.0003). The risk of dying during hospitalization is 20% higher for each point that the PROFUND index increases (OR 1.2, 95% CI 1.1-1.4). The area under the curve the receiver operating characteristic (AUC-ROC) of the PROFUND index to predict mortality during hospitalization (0.760, 95% CI 0.628-0.891) was higher than that of the RDW, CRP and albumin (0.494 95% CI 0.364-0.624 p 0.012; 0.583 95% CI 0.437-0.728 p 0.028; 0.621 0.494-0.748 p 0.109, respectively). The PROFUND index is associated with in-hospital mortality, with a greater predictive capacity than the biomarkers studied, which would add to its long-term prognostic value in multiple pathological patients.

Resumen El índice PROFUND se desarrolló y validó para predecir mortalidad a 12 meses en pacientes pluripatológicos. Sin embargo, su valor potencial para predecir mortalidad intrahospitalaria no ha sido suficientemente estudiado. Se evaluó la capacidad del índice PROFUND en comparación con la proteína C re activa (PCR), la albúmina, y el ancho de distribución eritrocitaria (ADE) para predecir mortalidad intrahospitalaria, mediante el análisis posterior de una cohorte prospectiva de 111 pacientes pluripatológicos internados en clínica médica. La edad promedio fue 75.8 ± 9.3 años. La mortalidad intrahospitalaria fue de 17% (19 pacientes). La mediana (RIQ) del índice PROFUND, albúmina, PCR y ADE en los fallecidos y sobrevivientes fue 12 (4) y 6 (7) p< 0.0001, 2.5 (0.4) y 2.6 (0.8) p 0.295, 58 (64) y 40 (60) p 0.176, 14.5 (2) y 14.6 (3) p 0.523, respectivamente. El análisis logístico multivariado mostró que el índice PROFUND se asocia con mortalidad intrahospitalaria (p 0.0003). El riesgo de fallecer durante la internación es 20% mayor por cada punto que se incrementa el índice PROFUND (OR 1.2, IC95% 1.1-1.4). El área bajo la curva de las características operativas del receptor (AUC-ROC) del índice PROFUND para predecir mortalidad durante la internación (0.760, IC95% 0.628-0.891) fue mayor a la del ADE, PCR y albúmina (0.494 IC95% 0.364-0.624 p 0.012; 0.583 IC95% 0.437-0.728 p 0.028; 0.621 0.494-0.748 p 0.109, respectivamente). El índice PROFUND se asocia a mortalidad intrahospitalaria, con una mayor capacidad predictiva que los biomarcadores estudiados, lo cual se sumaría a su valor pronóstico a largo plazo en pacientes pluripatológicos.

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14.

Enfermedad residual medible mediante reordenamientos de inmunoglobulinas y receptores de linfocitos-T
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Rubio, Patricia

; Alonso, Cristina N.

; Ducatelli, Maria E.

; Rossi, Jorge

; Guitter, Myriam

; Felice, Maria S.

Medicina (Buenos Aires)

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jun 2021, Volume 81 N. 3 Pages 337 - 345

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Acute leukemias are the most common neoplasm in pediatric patients. Currently, 80% of children with diagnosis of acute lymphoblastic leukemia (ALL) are cured with conventional chemotherapy, but 20% of them will have a recurrence of the disease. Measurable Residual Disease (MRD) has been described as an important prognostic factor that allows evaluating the response of patients to treatment. One of the most sensitive techniques to study MRD is the quantification of immunoglobulins (Ig) and T-lymphocyte receptors (TCR) genes rearrangements. The aims of this study were to describe the detected Ig/TCR rearrangements, to evaluate the prognostic impact of MRD in our population of children with ALL and to compare the MRD values by Ig/TCR with those obtained by multiparametric flow cytometry (MFC). A total of 455 patients were studied. In 96% of the cases, it was possible to characterize at least one Ig/TCR rearrangement. The total number of Ig/TCR rear rangements detected was 1550. MRD was successfully applied in 89% of the cases. The combined positive MRD values at day 33 and 78 of treatment allow the identification of high-risk patients in cases previously stratified by MRD using flow cytometry at day 15. The comparison between MRD determination by Ig/TCR rearrangements and FC showed excellent correlation. The present work constitutes a study of MRD by Ig/TCR carried out in a very significant number of patients consecutively diagnosed, treated within a homogeneous protocol and with excellent clinical follow-up.

Resumen Las leucemias agudas constituyen la neoplasia más frecuente en pacientes pediátricos. Actualmente, el 80% de los niños con leucemia linfoblástica aguda (LLA) logran curarse con quimioterapia con vencional pero el 20% de los mismos presentarán una reaparición de la enfermedad. La enfermedad residual medible (ERM) ha sido descripta como un importante factor pronóstico, que permite evaluar la respuesta de los pacientes al tratamiento. Una de las técnicas más sensibles par a estudiar ERM es la cuantificación de reordena mientos génicos de inmunoglobulinas (Ig) y receptores de linfocitos-T (TCR). Los objetivos del presente trabajo fueron describir los reordenamientos detectados de Ig/TCR, evaluar el efecto de la ERM en la supervivencia de niños con LLA y comparar la ERM por Ig/TCR con la cuantificada mediante citometría de flujo multiparamétrica (CFM). Del total de 455 pacientes estudiados, en el 96% fue posible caracterizar al menos un reordenamiento de Ig/TCR. El total de reordenamientos clonales detectados fue de 1550. La ERM pudo ser estudiada en forma exitosa en el 89% de los casos. El valor de ERM positiva combinada al día 33 y 78 de tratamiento, permitió identificar pacientes de alto riesgo, entre los previamente estratificados por la ERM mediante CFM al día 15. La comparación entre la determinación de ERM mediante reordenamientos Ig/TCR y CFM mostró una excelente correlación. El presente trabajo constituye un estudio de ERM mediante Ig/TCR realizado en un número muy significativo de pacientes diagnosticados en forma consecutiva, tratados en el marco de un protocolo homogéneo y con excelente seguimiento clínico.

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15.

Aprender en la virtualidad
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Aveleyra, Ema E.

; Rossi, David S.

Revista Iberoamericana de Tecnología en Educación y Educación en Tecnología

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mar 2021, N. 28 Pages 403 - 408

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

The results obtained from the implementation of the distance learning modality in a physics subject corresponding to the School of Engineering of the University of Buenos Aires are presented. The technique used was the survey carried out on 1164 second-year students of engineering degrees, after having completed the course corresponding to the first four-month period in 2020. From the quantitative-qualitative analysis carried out, positive results were obtained about the implementation of the modality. Comments made by students, after answering the questions asked, justify addressing some methodological changes and some improvements on the role of facilitators to foster student-student and student-teachers interactions.

Se presentan los resultados obtenidos de la aplicaciÃ³n de la modalidad a distancia en una asignatura de fÃsica correspondiente a la Facultad de IngenierÃa de la Universidad de Buenos Aires. La tÃ©cnica usada fue la encuesta realizada sobre 1164 estudiantes de segundo aÃ±o de las carreras de ingenierÃa, luego de haber finalizado la cursada correspondiente al primer cuatrimestre de 2020. Del anÃ¡lisis cuanti-cualitativo, llevado a cabo, se obtienen resultados positivos de la implementaciÃ³n de la modalidad. Los comentarios vertidos por los estudiantes, que completan las respuestas a las preguntas realizadas, justifican abordar algunos cambios metodolÃ³gicos y la bÃºsqueda de facilitadores para la interacciÃ³n entre estudiantes y estudiantes-docentes.

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