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Educating diabetic patients through an SMS intervention: a randomized controlled trial at a Brazilian public hospital
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Aceti, Viviane Moura

; Santoro, Rodolpho Vianna

; Velarde, Luis Guillermo Coca

; Brandão, Diego Nunes

; Cruz Filho, Rubens Antunes da

; Taboada, Giselle F.

Archives of Endocrinology and Metabolism

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dec 2021, Volume 65 N. 6 Pages 695 - 703

Abstract: En | Text: En | PDF: En

ABSTRACT Objective: Diabetes mellitus (DM) has a high healthcare system cost worldwide. Educational strategies are important to improve self-care and control this disease. This study aimed to evaluate satisfaction and clinical efficacy of a Short Message Service (SMS) educational intervention in self-care and nutrition at a Brazilian university hospital. Materials and methods: We conducted a trial of educational intervention and assigned eligible patients with DM to either receive weekly educational SMS for 6 months (intervention group [IG]) or no SMS at all (control group). A satisfaction questionnaire was applied before and after the intervention in both groups. Laboratory (fasting glucose, hemoglobin [Hb] A1c, total cholesterol, triglycerides, high-density lipoprotein, and low-density lipoprotein) and clinical (blood pressure) data were also collected. Data were analyzed using nonparametric tests with the Statistical Package for the Social Sciences. Results: We included 128 patients (64 in each group). Responses to the satisfaction questionnaire with self-care and healthcare professionals from 112 patients revealed an improvement in the perception of receiving information regarding helpful eating habits and in healthy eating behavior and an improvement in satisfaction with their diabetes care in the IG. In the post-intervention period, improved systolic blood pressure and HbA1c levels were observed in the IG as illustrated by delta % (post-intervention minus pre-intervention data divided by pre-intervention data multiplied by 100) reductions of 2.3% and 3.9%, respectively Conclusion: SMS intervention was useful as an educational tool for improving satisfaction and glycemic and blood pressure control of patients with DM observed at a Brazilian university hospital.

https://doi.org/10.20945/2359-3997000000390 2 downloads

Diversity matters: opportunities in the study of the genetics of psychotic disorders in low- and middle-income countries in Latin America
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Fonseca, Lais

; Sena, Brena F.

; Crossley, Nicolas

; Lopez-Jaramillo, Carlos

; Koenen, Karestan

; Freimer, Nelson B.

; Bressan, Rodrigo A.

; Belangero, Sintia I.

; Santoro, Marcos L.

; Gadelha, Ary

Brazilian Journal of Psychiatry

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dec 2021, Volume 43 N. 6 Pages 631 - 637

Abstract: En | Text: En | PDF: En

Lack of diversity regarding genetic and environmental backgrounds weakens the generalization and clinical applicability of research findings on psychotic disorders. Notably, Latin Americans have been generally neglected in genetic studies, comprising less than 2% of genome-wide association study samples. But Latin American populations represent a unique opportunity for research, given the exceptionally high ethnic admixture of this group. Increasing genetic diversity is essential to improve the fine mapping of known regions associated with psychotic disorders, discover novel genetic associations, and replicate studies. Additionally, Latin America is characterized by massive social, political, and economic inequalities, all known risk factors for mental health issues, including psychotic disorders. This article aims to 1) discuss the challenges and advantages of studying Latin America’s particular genetic makeup and environmental context; 2) review previous studies conducted in the region; and 3) describe three Latin American research initiatives in progress: the Neuropsychiatric Genetics of Psychosis in Mexican Populations (NeuroMEX), the Paisa, and the Latin American Network for the Study of Early Psychosis (ANDES) studies.

https://doi.org/10.1590/1516-4446-2020-1240

A WORLDWIDE NETWORK FOR COMPARATIVE STUDIES ON CARAVANS: PAST, PRESENT AND FUTURE
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Clarkson, Persis B.

; Santoro, Calogero M.

; Levy, Thomas E.

; Núñez, Lautaro

; Nielsen, Axel

; Rosen, Steven

; Forster, Frank

; Capriles, José M.

; Khazanov, Anatoly M.

; Frachetti, Michael

; Valenzuela, Daniela

; Standen, Vivien G.

; Cases, Barbara

; Pimentel, Gonzalo

; Lecoq, Patrice

; Medinacelli, Ximena

; Briones, Luis

; Wink, André

; Tripcevich, Nicholas

; Riemer, Heiko

; O'Ryan, Enelidolfo

; Loayza, Ximena

; Lynch, Thomas F.

; Woldekiros, Helina

Chungará (Arica)

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sep 2017, Volume 49 N. 3 Pages 297 - 307

Abstract: En | Text: En | PDF: En

700 downloads

Mudanças nas características físico-químicas de um latossolo vermelho-amarelo distrófico após a irrigação com água de reúso na cultura da alface-crespa (Lactuca sativa, L.)
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Varallo, Antonio C. T.

; Souza, Claudinei F.

; Santoro, Bruno de L.

Engenharia Agrícola

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apr 2012, Volume 32 N. 2 Pages 271 - 279

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A utilização do efluente de esgoto tratado para poupar os corpos d'água utilizados para irrigação tem sido de grande interesse na agricultura, e o uso não regulamentado desta prática pode acarretar mudanças no comportamento físico-químico do solo. Sua prática tem sido de grande interesse em vista da redução da quantidade de água retirada dos mananciais, preservando-os. Objetivou-se, neste trabalho, a avaliação dos atributos físico-químicos de um Latossolo Vermelho-Amarelo após aplicação de água de reúso. O experimento foi realizado em uma condição de casa de vegetação onde foram cultivados dois ciclos da alface-crespa (Lactuca sativa, L) com dois tratamentos, com três repetições cada: testemunhas (água de abastecimento proveniente de poço) e reúso (proveniente de esgoto local). Os resultados mostraram que não ocorreram aumentos em alguns atributos químicos de fertilidade, como ferro, magnésio, zinco, boro, potássio, nitrato, sódio e cálcio; decréscimo nos valores de matéria orgânica e manganês, além de diminuição da condutividade hidráulica e aumento na condutividade elétrica do solo. Observou-se, ainda, redução do pH no solo, além do aumento da RAS e PST. Desta forma, concluiu-se que a utilização de água de reúso para fins agronômicos deve ser de forma racional, monitorando-se, principalmente, a elevação do teor de sais no solo.

The use of treated sewage effluent to save the ponds used for irrigation has been of great interest in agriculture, and the unregulated use of this practice can cause changes in physical and chemical behavior of the soil. This practice has been of great interest in view of reducing the quantity of water withdrawal from the springs and its preservation. The objective of this study is to evaluate the physical and chemical attributes of a Red-yellow Dystrophic Latosol after the implementation of water reuse. The experiment was conducted in a condition of greenhouse where were grown two rounds of curly lettuce (Lactuca sativa, L. ) with two treatments with three repetitions each: witness (provisioning water) and reuse. The results did not show increases in some chemical attributes of fertility as iron, magnesium, zinc, boron, potassium, nitrate, sodium and calcium; decrease in organic matter and manganese, besides increase of hydraulic conductivity and the electrical conductivity of the soil. It was also observed a reduction of the soil pH, besides the increase of SAR and ESP. Thus it must be concluded the application of water reuse for agricultural purposes should be in a rational way, and the elevation of salts in the soil must be monitored.

3429 downloads

Utilização da TDR para monitoramento da solução de nitrato de potássio em Latossolo Vermelho-Amarelo
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Lopes, Leonardo do N.

; Souza, Claudinei F.

; Santoro, Bruno de L.

Engenharia Agrícola

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oct 2010, Volume 30 N. 5 Pages 932 - 947

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O conhecimento da distribuição e armazenamento da solução no solo é de grande importância para a agricultura, pois a interação entre os nutrientes e a água é um dos fatores que influenciam diretamente no rendimento das culturas. Das várias técnicas utilizadas para o monitoramento da solução no solo, a reflectometria no domínio do tempo (TDR) vem sendo bastante difundida entre os pesquisadores por apresentar inúmeras vantagens, dentre as quais a mensuração em tempo real e a possibilidade de leituras automatizadas. O principal objetivo desta pesquisa foi avaliar a distribuição da solução de KNO3 no perfil de um Latossolo Vermelho-Amarelo. Sondas de Reflectometria no Domínio do Tempo (TDR) foram utilizadas para monitorar a distribuição de solução no solo aplicada por gotejadores de fluxo constante nas vazões de 2; 4 e 8 L h-1. Considerando-se os resultados de diferentes perfis, observou-se maior armazenamento da solução próxima ao gotejador, diminuindo progressivamente para frente de molhamento. Pouco mais da metade da solução aplicada (65%) foi armazenada na primeira camada (0-0,10 m) para todos os ensaios, e 22% foi armazenada na próxima camada (0,10-0,20 m). Comparando-se diferentes taxas de aplicação, observou-se maior armazenamento de água para o gotejador de 4 L h-1, com 60; 72 e 63% de solução de KNO3 aplicada acumulada na primeira camada (0-0,10 m) para gotejadores de 2; 4 e 8 L h-1, respectivamente. Os resultados sugerem que, com base no volume e frequência utilizada neste experimento, seria vantajoso aplicar pequenas quantidades de água em intervalos mais frequentes para reduzir perdas por percolação.

Knowledge of water distribution in soil is of great importance to agriculture, since water is one of the factors that most influence the yield of crops. There are many techniques used for monitoring of soil water content, the Time Domain Reflectometry (TDR) has been widespread among researchers to present several advantages, among which the determination in real time and possibility of automated readings. The main goal of this research was to evaluate the KNO3 solution distribution in a profile of a Dystrophic Red-Yellow latossol. Time domain reflectometry (TDR) probes were used to monitor the soil solution distribution from drippers discharging at constant flow rates of 2, 4 and 8 L h-1 in soil. Considering the results from different profiles, we observed greater solute storage near the dripper decreasing gradually towards the wetting front. About half of the applied KNO3 solution (65%) was stored in the first layer (0-0.10 m) for all experiments and 22 % was stored in the next layer (0.10-0.20 m). Comparing different dripper flow rates, we observed higher solution storage for 4 L h-1, with 60, 72 and 63 % of the applied KNO3 solution accumulated in the first layer (0-0.10 m) for dripper flow rates of 2, 4 and 8 L h-1, respectively. The results suggest that based on the volume and frequency used in this experiment, it would be advantageous to apply small amounts of solution at more frequent intervals to reduce deep percolation losses of applied water and solutes.

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Alterações nos atributos de um Latossolo Vermelho-amarelo irrigado com água de reúso
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Varallo, Antonio C. T.

; Carvalho, Letícia

; Santoro, Bruno L.

; Souza, Claudinei F.

Revista Brasileira de Engenharia Agrícola e Ambiental

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apr 2010, Volume 14 N. 4 Pages 372 - 377

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A reutilização do efluente de esgoto tratado para suprir a falta de fontes d'água para irrigação tem sido de grande interesse para a agricultura e o uso desta prática, se não regulamentado, pode acarretar em mudanças no comportamento físico e químico do solo. Objetivou-se, neste trabalho, a avaliação das alterações dos atributos físico-químicos de um Latossolo Vermelho amarelo, após a aplicação da água de reúso proveniente de uma estação de tratamento de efluentes por leitos cultivados. Um experimento em coluna de solo foi realizado em laboratório; 15 L de água destilada lhe foram aplicados e, logo após, mais 15 L de água de reúso e, a cada 5 L, foi coletada amostra da água lixiviada para análises. Os resultados mostraram, após a aplicação no solo, da água de reúso, redução de nitrato, potássio e valores da condutividade hidráulica saturada e aumento das concentrações de sódio, ferro, manganês, zinco e condutividade elétrica. Pode-se concluir, desta forma, que a utilização de água de reúso para fins agronômicos deve ser de forma racional, monitorando-se principalmente a elevação do teor do íon sódio.

The reuse of treated sewage effluent to water bodies to supply water for irrigation has been of great advantage to agriculture, and the use if not regimented could bring changes in physical and chemical properties of soil. This paper aimed the evaluation of the physical and chemical properties alteration of a Red-yellow Latosol after the application of reuse water from an effluent treatment station of wetlands. The experiment was accomplished in laboratory using a soil column. First, 15 L of distilled water were applied in the soil column and after 15 L of reuse water and at interval of 5 L of water a sample was collected for analysis. The results showed decrease of nitrate, potassium and saturated hydraulic conductivity values and an increase in sodium, iron, manganese, zinc and electrical conductivity of the soil after water application. In this way, it may be concluded that the use of waste water for agronomic activities must be used in a rational way, mainly checking the elevation of sodium.

4310 downloads

Distribuição de água no solo aplicado por gotejamento enterrado e superficial
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Barros, Allan C.

; Folegatti, Marcos V.

; Souza, Claudinei F.

; Santoro, Bruno L.

Revista Brasileira de Engenharia Agrícola e Ambiental

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dec 2009, Volume 13 N. 6 Pages 700 - 707

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Devido à falta de estudos sobre o movimento da água quando aplicada abaixo da superfície, realizou-se este trabalho com o objetivo de avaliar a distribuição de água aplicada pelo sistema de gotejamento enterrado e convencional. O experimento de campo foi conduzido na área experimental do Departamento de Engenharia Rural da ESALQ/USP - Piracicaba, SP. Para o estudo, trincheiras foram abertas e instaladas sondas de TDR, dispostas a 0,05; 0,15; 0,25; 0,35 e 0,45 m profundidade, e a 0,05; 0,15; 0,25; 0,35 m comprimento, totalizando 17 sondas por trincheira. Os tratamentos foram baseados na profundidade de aplicação (0,0 e 0,10 m) e vazão aplicada (2 e 4 L h-1): ENT2; ENT4; SUP2 e SUP4. A cada hora era aplicado 1 L de água (total de 10 L), seguida de leituras com o TDR. Medições do disco úmido e saturado foram feitas com régua milimetrada; além disso, estabeleceu-se um volume controle onde foi avaliada a uniformidade de aplicação; assim, foi possível verificar, em relação aos sistemas superficiais, que os sistemas enterrados apresentaram menor área superficial molhada e atingiram maior largura e profundidade; já as maiores concentrações foram obtidas próximas ao ponto de emissão.

Studies of sub-surface water movement is an interesting topic in irrigation but, in spite of the its importance, there is little literature. One of the purposes of this study was to contribute to this subject and evaluate the distribution of water applied by both subsurface drip irrigation (SDI) and conventional irrigation. Experiments were conducted at the Department of Rural Engineering (ESALQ/USP), located at Piracicaba, SP. Trenches were opened and 17 three-rod TDR probes were installed, placed at 0.05, 0.15, 0.25, 0.35 and 0.45 m depths, and to 0.05, 0.15, 0.25, 0.35 m intervals. This procedure was repeated using a dripper buried at 0 and 0.10 m for each discharge rate of 2 and 4 L h-1 tested. Wetted soil volume was observed with 1 L of water applications at intervals (total 10 L). The saturated disc around the dripper and the advance of the wetting front on the soil surface were measured with a ruler. Also, a central control volume was established inside the wetted soil volume where the uniformity of application was evaluated. The results suggest that SDI presented smaller wetted soil surface area and it reached larger distance and depth with greater storage of solution adjacent to the dripper.

4874 downloads

Influence of age, gender and educational level on performance in the Brief Cognitive Battery-Edu
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Nitrini, Ricardo

; Brucki, Sonia Maria Dozzi

; Smid, Jerusa

; Carthery-Goulart, Maria Teresa

; Anghinah, Renato

; Areza-Fegyveres, Renata

; Bahia, Valeria Santoro

; Damin, Antonio Eduardo

; Formigoni, Ana Paula

; Frota, Norberto Anízio Ferreira

; Guariglia, Carla

; Jacinto, Alessandro F.

; Kato, Eliane Mayme

; Lima, Edson Erasmo Pereira

; Moreira, Daniel

; Nóbrega, Ana Beatriz

; Porto, Claudia Sellitto

; Senaha, Mirna Lie Hosogi

; Silva, Mari Nilva M.

; Souza-Talarico, Juliana Neri

; Radanovic, Marcia

; Mansur, Leticia Lessa

Dementia & Neuropsychologia

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jun 2008, Volume 2 N. 2 Pages 114 - 118

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo A Bateria Breve de Rastreio Cognitivo - Edu (BBRC-Edu) contém nove testes, sete dos quais relacionados à memória de desenhos. Tem uma boa acurácia no diagnóstico de comprometimento cognitivo. Objetivos: Avaliar a influência da idade, sexo e nível educacional no desempenho nos testes relacionados à memória de desenhos da BBRC-Edu em sujeitos saudáveis. Métodos: Os participantes foram voluntários; os critérios de exclusão foram: analfabetismo, doenças neurológicas ou psiquiátricas, perda auditiva ou visual, condições clínicas descompensadas, etilismo, uso de drogas, e para aqueles com idade igual ou superior a 65 anos, informação de acompanhante de presença de comprometimento cognitivo ou funcional. Foram avaliados 325 indivíduos (207 mulheres), com idade média de 47.1 (±16.8) anos, variando de 19 a 81 nos, média de 9.8 (±5.0) anos de escolaridade. Análise univariada, correlações e regressão logística foram empregadas (a=0.05). Resultados: Houve correlações negativas significativas entre idade e os escores de quatro dos sete testes. A escolaridade correlacionou-se positivamente aos escores, embora a escolaridade diminua com a idade nesta amostra (rho= -0.323; p<0.001). Regressão Logística confirmou que o gênero tem influência no aprendizado, com melhor desempenho entre as mulheres, enquanto a idade influenciou a memória incidental, memória imediata, aprendizado e evocação das figuras; e a escolaridade influenciou a identificação, memória imediata, aprendizado, evocação e reconhecimento. Conclusão: Gênero, idade e educação tiveram influência no desempenho na memória de figuras da BBRC-Edu, embora de modo diferente entre os tipos de teste.

Abstract The Brief Cognitive Battery-Edu (BCB-Edu) contains nine tests, seven of which are related to the memory of drawings, and has good accuracy in the diagnosis of cognitive impairment. Objectives: To evaluate the influence of age, gender and educational level on the performance in tests related to memory of drawings of the BCB-Edu in healthy subjects. Methods: Participants were adult volunteers; exclusion criteria were illiteracy, neurologic or psychiatric disorders, visual or hearing impairment, untreated chronic clinical conditions, alcoholism, use of drugs, and for those aged 65 or over, an informant report of cognitive or functional impairment. We evaluated 325 individuals (207 women), with a mean age of 47.1 (±16.8) years, ranging from 19 to 81, and a mean of 9.8 (±5.0) schooling-years. Univariate analyses, correlations and logistic regression were employed (a=0.05). Results: There were significant negative correlations between age and the scores in four of the seven tests. However, schooling-years were positively correlated to the scores, where schooling-years decreased with age in this sample (rho= -0.323; p<0.001). Logistic regression confirmed that gender influenced the learning of drawings, where women performed better, while age influenced incidental memory, immediate memory, learning and delayed recall of the drawings, and schooling-years influenced visual identification, immediate memory, learning, delayed recall and recognition of the drawings. Conclusion: Gender, age and education influence the performance on the memory of drawings of the BCB-Edu, although the extent of these influences differs according to the nature of the test.

https://doi.org/10.1590/S1980-57642009DN20200007 1894 downloads

Aspectos biológicos e moleculares de amostras uropatogênicas de Escherichia coli isoladas na Cidade do Rio de Janeiro
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Esparis, Carmen Macedo

; Teixeira, Lúcia Martins

; Irino, Kinue

; Gil, Paola F.

; Almeida, Monica M.T. Barros

; Lopes, Guilherme Santoro

; Bravo, Vera Lúcia R.

; Pacheco, Raquel S.

; Regua-Mangia, Adriana Hamond

Revista da Sociedade Brasileira de Medicina Tropical

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dec 2006, Volume 39 N. 6 Pages 573 - 576

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Amostras uropatogênicas de Escherichia coli isoladas de indivíduos moradores de localidades distintas na Cidade do Rio de Janeiro, foram caracterizadas quanto o sorotipo, propriedades hemolíticas e hemaglutinantes, susceptibilidade a antimicrobianos e perfil isoenzimático. O método molecular empregado associado com a investigação de marcadores de urovirulência, permitiu detectar uma grande diversidade entre os isolados. Entretanto, foi observada uma relação mais estreita entre amostras de Escherichia coli epidemiologicamente relacionadas.

Uropathogenic Escherichia coli strains isolated from individuals living in different areas of the city of Rio de Janeiro were characterized according to serotype, hemolytic properties, hemagglutination properties, antimicrobial susceptibility and isoenzymatic profile. The molecular approach used, together with investigation of urovirulence markers, enabled detection of great diversity among the isolates. However, closer relationships were observed between epidemiologically related Escherichia coli samples.

6177 downloads Cited 2 times in SciELO

10.

Co-Editorial
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Femandes, Flávio César F.

; Moccellin, João Vitor

; Santoro, Miguel Cezar

; França, Paulo Morelato

; Armentano, Vinicius Amaral

Gestão & Produção

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dec 2000, Volume 7 N. 3 Pages i - i

Abstract: Pt | Text: Pt | PDF: Pt

1032 downloads

11.

The subclinical form of experimental visceral leishmaniasis in dogs
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Oliveira, G. G. S.

; Santoro, F.

; Sadigursky, M.

Memórias do Instituto Oswaldo Cruz

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jun 1993, Volume 88 N. 2 Pages 243 - 248

Abstract: En | Text: En | PDF: En

Pathological aspects of a subclinical form of experimental canine leishmaniasis is reported here for the first time. Fifteen mongrel dogs were used in the present study. Eight dogs were infected and seven were used as control. Four of the control dogs were inoculated with spleen cells from non-infected hamsters. The eight mongrel dogs inoculated intravenously with amastigotes forms of Leishmania chagasi envolved for periods as long as 25 months without any clinical characteristic sign of classical Visceral Leishmaniasis (VL). Most of the laboratory test results were compatible to those of the seven control animals but culture of bone marrow aspirated material and serologic testing (IIF) demonstrated or provided evidence that the animals were infected. The most important and predominant histopathological lesion in infected animals were epitheloid granulomas presented in the liver, spleen, adrenal gland and lung of some animals. Channels containing erythrocytes in some granulomas of the liver suggeste that these granulomas are formed inside sinusoidal capillaries. Despite the animals were proved to be infected and presented characteristic histologic lesions, they did not present external signs of disease. The granulomatous aspect of the lesions indicates a good immunologic reactivity and suggest that a host-parasite equilibrium does exist in the dog experimental model

1734 downloads Cited 1 time in SciELO

12.

Evaluation of spleen cell population and effect of splenectomy on granuloma modulation in BALB/c mice infected with Schistosoma mansoni
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Arruda, S. M.

; Santoro, F.

; Sadigursky, M.

Memórias do Instituto Oswaldo Cruz

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mar 1993, Volume 88 N. 1 Pages 97 - 102

Abstract: En | Text: En | PDF: En

A kinetic study of the cells present in the spleen of BALB/c mice infected with Schistosoma mansoni was carried out. The lymphocytes were evaluated phenotypically with monoclonal antibodies and the effect of splenectomy on the modulation of periovular granuloma was also investigated. The infected mice had proportional increases in the numbers of neutophils, plasma cells, macrophages and eosinophils in the spleen. The largest number of neutrophil, plasma cells and macrophage were found between the 8th and the 12th week of infection, while the amount of eosinophils were higher later on, around the 20th week. The lymphocytes phenotipically characterized as Thy 1.2, Lyt 1.2 (CD4) increased mildly in proportional numbers. However, the percentage of lymphocytes with the Lyt 2.2 (CD8) phenotype, which is characteristic of supressor and cytotoxic T cells, increased significantly with the progress of the disease. The numbers of B lymphocytes, which comprise 50% of the mononuclear cells present in the spleen, increased significantly till the 16th week they began to decrease. The mean diameters of periovular granulomas were comparatively similar in both experimental groups (splenectomized and non-splenectomized mice). However, the evolutive types of granuloma (exudative, intermediate and fibrous) in splenectomized mice were proprtionally different from those of non splenectomized mice in the 16th and 24th week of infection. It is inferred that lymphonodes or other secondary lymphoide organs, in the abscence of the spleen, assume a modulating action on periovular granulomas, although the evolution of the granulomas is somehow delayed in splenectomized mice.

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13.

Studies on anti-component 5 antibodies in animals infected with Trypanosoma cruzi
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Santoro, F.

; Sadigursky, M.

; Sherlock, I.

; Afchain, D.

; Capron, A.

Revista da Sociedade Brasileira de Medicina Tropical

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jun 1987, Volume 20 N. 2 Pages 91 - 94

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Foi usado um teste imunoenzimático competitivo para investigar a presença de anticorpos anticomponente 5 nos soros de sariguês, cães, coelhos e ratos infectados com o Trypanosoma cruzi. Neste teste, foi utilizado um anticorpo monoclonal contra o antígeno 5 que é específico do T. cruzi. Também foram testados os soros de 51 pacientes venezuelanos com doença de Chagas. Apesar desses anticorpos não serem detectados nos soros de cães, ratos e sariguês infectados com o T. cruzi, alguns soros de coelhos infectados apresentaram resultados positivos porém em níveis próximos aos do limite de positividade do teste. Esses achados sugerem que a resposta imune em animais naturalmente ou experimentalmente infectados com o T. cruzi é diferente daquela que é observada na doença de Chagas humana.

A competitive antibody enzyme immunoassay, using a monoclonal antibody against the species-specific Trypanosoma crnzi antigen 5, was used to investigate the presence of anti-component 5 antibodies in sera of opossums, dogs, rabbits and rats infected with this parasite. The sera from 51 Venezuelan patients with Chagas’disease were also tested. About 90% of the infected subjects showed significant levels of anti-component 5 antibodies. Nevertheless, these antibodies were not detected in the sera of dogs, rats and opossums infected with T. cruzl Some sera from infected rabbits presented significant results but close to the limit ofpositivity ofthe test. These findings suggest that the immune response in animals naturally or experimentally infected with T. cruzi is different from that observed in human Chagas’disease.

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