Lack of diversity regarding genetic and environmental backgrounds weakens the generalization and clinical applicability of research findings on psychotic disorders. Notably, Latin Americans have been generally neglected in genetic studies, comprising less than 2% of genome-wide association study samples. But Latin American populations represent a unique opportunity for research, given the exceptionally high ethnic admixture of this group. Increasing genetic diversity is essential to improve the fine mapping of known regions associated with psychotic disorders, discover novel genetic associations, and replicate studies. Additionally, Latin America is characterized by massive social, political, and economic inequalities, all known risk factors for mental health issues, including psychotic disorders. This article aims to 1) discuss the challenges and advantages of studying Latin America’s particular genetic makeup and environmental context; 2) review previous studies conducted in the region; and 3) describe three Latin American research initiatives in progress: the Neuropsychiatric Genetics of Psychosis in Mexican Populations (NeuroMEX), the Paisa, and the Latin American Network for the Study of Early Psychosis (ANDES) studies.

Abstract Denture adhesives (DA) improve the retention and stability of ill-fitting dentures, especially for older adults. These materials should be biocompatible, i.e., they cannot cause undesired biological responses and be non-cytotoxic to oral tissues. However, in vitro testing of DA biocompatibility employing primary cell culture may possibly be affected by other factors, such as the donor age. Objective To compare the cytotoxicity of three different denture adhesives when assessed in primary gingival fibroblasts from a young donor or from an older donor, as well as the release of the basic fibroblast growth factor (bFGF), and the inflammatory response marker interleukin-6 (IL-6). Material and Methods Gingival fibroblasts isolated from a 30- and a 62-year-old donor were assayed for proliferation (1-7 days) and sensitivity to latex (positive control). Fibroblasts were indirectly exposed to Corega Ultra (cream), Corega powder and Fixodent Original for a 24 h period and assayed by XTT and Crystal Violet tests. The release of IL-6 and bFGF by exposed cells was determined by ELISA. Results While cells from the young donor presented higher cell growth after 7 days, the sensitivity to increasing concentrations of latex extracts was very similar between young and older cells. Both XTT and CVDE detected no difference between the DA and the control group. All materials induced higher levels of IL-6 and bFGF compared to control. Cells from the older donor exposed to Corega Ultra released lower levels of cytokine and growth factor. Conclusions All materials were considered non-cytotoxic, but affected cytokine and growth factor release. The biological differences found between fibroblasts from both donors could be due to individual or age-related factors. The authors suggest the use of cells from older donors on studies of dental products aimed at older patients, to better simulate their physiological response.

The isothermal crystallization kinetics of PLA stereocomplex (scPLA) and scPLA/graphene oxide nanocomposites was investigated. scPLA was prepared by symmetric blending the two isomers, PLLA and PDLA and the scPLA nanocomposite by polymerization of the respective lactides in presence of commercial graphene oxide nanoplatelets (GNPO+) followed of blending the two composites. The effect of the filler on the crystallization behavior and thermal properties of scPLA was investigated by using differential scanning calorimetry (DSC). Avrami equation was used to describe the isothermal crystallization kinetics and melting behavior. Addition of 0.5 wt% GNPO+ to PLA matrix increases the crystallization rate, lowering the half-time of crystallization t1/2 to around 5.5 min for scPLA nanocomposites, which is about 2.7 min below that found for scPLA when isothermally crystallized at 165ºC. During the isothermal crystallization, homochiral and stereocomplex crystals are formed, depending on the crystallization temperature. As crystallization temperature reaches 160 and 165ºC, the crystallization of homocrystals become prevented and only stereocomplex crystals are formed.

Devido à falta de estudos sobre o movimento da água quando aplicada abaixo da superfície, realizou-se este trabalho com o objetivo de avaliar a distribuição de água aplicada pelo sistema de gotejamento enterrado e convencional. O experimento de campo foi conduzido na área experimental do Departamento de Engenharia Rural da ESALQ/USP - Piracicaba, SP. Para o estudo, trincheiras foram abertas e instaladas sondas de TDR, dispostas a 0,05; 0,15; 0,25; 0,35 e 0,45 m profundidade, e a 0,05; 0,15; 0,25; 0,35 m comprimento, totalizando 17 sondas por trincheira. Os tratamentos foram baseados na profundidade de aplicação (0,0 e 0,10 m) e vazão aplicada (2 e 4 L h-1): ENT2; ENT4; SUP2 e SUP4. A cada hora era aplicado 1 L de água (total de 10 L), seguida de leituras com o TDR. Medições do disco úmido e saturado foram feitas com régua milimetrada; além disso, estabeleceu-se um volume controle onde foi avaliada a uniformidade de aplicação; assim, foi possível verificar, em relação aos sistemas superficiais, que os sistemas enterrados apresentaram menor área superficial molhada e atingiram maior largura e profundidade; já as maiores concentrações foram obtidas próximas ao ponto de emissão.

Studies of sub-surface water movement is an interesting topic in irrigation but, in spite of the its importance, there is little literature. One of the purposes of this study was to contribute to this subject and evaluate the distribution of water applied by both subsurface drip irrigation (SDI) and conventional irrigation. Experiments were conducted at the Department of Rural Engineering (ESALQ/USP), located at Piracicaba, SP. Trenches were opened and 17 three-rod TDR probes were installed, placed at 0.05, 0.15, 0.25, 0.35 and 0.45 m depths, and to 0.05, 0.15, 0.25, 0.35 m intervals. This procedure was repeated using a dripper buried at 0 and 0.10 m for each discharge rate of 2 and 4 L h-1 tested. Wetted soil volume was observed with 1 L of water applications at intervals (total 10 L). The saturated disc around the dripper and the advance of the wetting front on the soil surface were measured with a ruler. Also, a central control volume was established inside the wetted soil volume where the uniformity of application was evaluated. The results suggest that SDI presented smaller wetted soil surface area and it reached larger distance and depth with greater storage of solution adjacent to the dripper.