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BACKGROUND The high demand for adequate material for the gold standard reverse transcription real-time polymerase chain reaction (RT-qPCR)-based diagnosis imposed by the Coronavirus disease 2019 (COVID-19) pandemic, combined with the inherent contamination risks for healthcare workers during nasopharyngeal swab (NP) sample collection and the discomfort it causes patients, brought the need to identify alternative specimens suitable for the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). OBJECTIVES The aim of this work was to compare saliva and gingival fluid swabs to NP swabs as specimens for RT-qPCR-based SARS-CoV-2 diagnosis. METHODS We compared gingival fluid swabs (n = 158) and saliva (n = 207) to the rayon-tipped NP swabs obtained from mild-symptomatic and asymptomatic subjects as specimens for RT-qPCR for SARS-CoV-2 detection. FINDINGS When compared to NP swabs, gingival fluid swabs had a concordance rate of 15.4% among positive samples, zero among inconclusive, and 100% among negative ones. For saliva samples, the concordance rate was 67.6% among positive samples, 42.9% among inconclusive, and 96.8% among negative ones. However, the concordance rate between saliva and NP swabs was higher (96.9%) within samples with lower cycle threshold (Ct) values (Ct > 10 ≤ 25). MAIN CONCLUSIONS Our data suggests that whereas gingival fluid swabs are not substitutes for NP swabs, saliva might be considered whenever NP swabs are not available or recommended.

https://doi.org/10.1590/0074-02760210085 205 downloads

Schistosoma mansoni granulomas in the skeletal striated muscles in the murine model of neuroschistosomiasis: histological findings
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Fidelis, Thiago Andre Alves

; Brasileiro-Filho, Geraldo

; Parreiras, Patricia M

; Coelho, Paulo Marcos Z

; Araujo, Neusa

; Chaud, Marco Vinicius

; Baldo, Denicezar Angelo

; dos Santos, Nelson Brancaccio

; Lambertucci, José Roberto

Memórias do Instituto Oswaldo Cruz

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2020, Volume 115 elocation e190383

Abstract: En | Text: En | PDF: En

Schistosomiasis mansoni presents many clinical manifestations during migration of schistosomes in their hosts, including diarrhea, hepatomegaly, splenomegaly, liver abscesses, skinlesions, brain tumors and myeloradiculopathy. No lesions have been reported in skeletal striated muscles due to schistosomiasis mansoni in the literature. This short communication reports the histopathological findings on skeletal musculature in a murine model of neuroeschistosomiasis mansoni. Lesions were found in the tongue, masseter muscle, buccinator muscle, digastric muscle and temporalis muscle. Worm recovery was carried out to confirm the infection. We describe here, for the first time in the literature, injuries in the skeletal musculature due to Schistosoma mansoni nfection.

https://doi.org/10.1590/0074-02760190383 621 downloads

Tumor de corpo carotídeo (paraganglioma): relato de dois casos submetidos a tratamento cirúrgico
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Mesquita Junior, Nelson

; Silva, Rogério Santos

; Ribeiro, José Henrique Agner

; Batista, Lislaine Cruz

; Bringhentti, Emanuelle Melania Stedille

; Souza, Bruno Benjamin Brunini de

; Cabral, Lisiane Cristine da Mota

Jornal Vascular Brasileiro

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jun 2016, Volume 15 N. 2 Pages 158 - 164

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo O tumor de corpo carotídeo é uma neoplasia rara, geralmente benigna, que acomete, sobretudo, indivíduos entre a quarta e a quinta décadas de vida. Manifesta-se pela presença de massa cervical consistente localizada abaixo do ângulo da mandíbula, pulsátil e comumente indolor. Pode evoluir para dor local, disfagia, soluços, rouquidão e síndrome do corpo carotídeo hipersensível. Este artigo relata os casos de duas pacientes diagnosticadas com essa neoplasia e submetidas ao tratamento cirúrgico. A primeira foi submetida a uma ressecção em bloco do tumor, enquanto a segunda, com estadiamento mais precoce, foi tratada com uma ressecção subadventicial da lesão.

Abstract A carotid body tumor is a rare neoplasm, generally benign, that predominantly affects people between their fourth and fifth decades of life. It manifests as a pulsatile and generally painless cervical mass with firm consistency, located below the angle of the jaw. It can progress to the extent that it causes localized pain, dysphagia, hiccups, hoarseness and hypersensitive carotid body syndrome. This article reports the cases of two female patients diagnosed with this tumor who were treated surgically. The first was treated with block resection of the tumor, while the second patient, who had an early stage tumor, was treated with subadventitial resection of the lesion.

https://doi.org/10.1590/1677-5449.007315 15782 downloads

Dinâmica da regeneração natural em uma floresta baixa de restinga degradada
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Oliveira, Marcelo Augusto Meratti de

; Oliveira, André Cruz de

; Rossi, Lucia

; Catharino, Eduardo Luis Martins

; Gomes, Eduardo Pereira Cabral

; Santos Junior, Nelson Augusto dos

Hoehnea

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dec 2015, Volume 42 N. 4 Pages 759 - 774

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Este estudo teve como objetivo caracterizar a composição da chuva e banco de sementes em uma floresta baixa de restinga degradada, a fim de verificar o aporte e estabelecimento dos diásporos na área e a contribuição dos remanescentes próximos para a regeneração natural. Num trecho degradado em Ilha Comprida, dez coletas mensais (1 m2 cada) para a chuva de sementes e trimestrais (0,25 m2 cada) para o banco de sementes foram realizadas durante um ano; e num remanescente vizinho amostraram-se indivíduos arbóreos com DAP ≥ 3 cm no estudo fitossociológico. Amostraram-se 1.627 diásporos na chuva de sementes, 2.986 no banco e 356 indivíduos no estudo fitossociológico. A presença de Clusia criuva, Gaylussacia brasiliensis, Myrcia ilheoensis, Tapirira guianensis e Tibouchina clavata na chuva e banco de sementes, consideradas espécies nucleadoras, de elevado potencial biótico ou pouca limitação de dispersão, podem possibilitar a sucessão na área. A densidade e qualidade de espécies que chegam por meio da chuva de sementes e que se estabelecem no banco de sementes indicam que a área pode se recuperar via regeneração natural.

ABSTRACT This study aimed to characterize the composition of the seed rain and the seed bank in a degraded low sandbank forest to verify the inflow and establishment of the seed diaspores in the area under evaluation, its natural regeneration and contribution of the nearby remnants. At Ilha Comprida, in a degraded site, ten samples were taken monthly (1 m2 each) for the seed rain and quarterly (0.25 m2 each) for the seed bank, during the period of a year. In a the nearby remnant, arboreous individual with DBH ≥ 3 cm were sampled in the phytosociology study. During the specified period, 1627 diaspores were sampled in the seed rain and 2986 diaspores in the seed bank, and 356 individuals were sampled in the phytosociology study. The presence of Clusia criuva, Gaylussacia brasiliensis, Myrcia ilheoensis, Tapirira guianensis, and Tibouchina clavata in the seed rain and seed bank, considered nucleation species, with high biotic potential or little limitation of dispersion, can allow successional development in the area. The density and quality of species arriving and settling in the area have the potential to help it regenerate naturally.

https://doi.org/10.1590/2236-8906-09/RAD/2015 2991 downloads

Influenza A virus infection of healthy piglets in an abattoir in Brazil: animal-human interface and risk for interspecies transmission
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Amorim, Ariane Ribeiro

; Fornells, Luz Alba Maria Garcete

; Reis, Felicidade da Costa

; Rezende, Daiana Jacinto

; Mendes, Gabriella da Silva

; Couceiro, José Nelson dos Santos Silva

; Santos, Norma Suely de Oliveira

Memórias do Instituto Oswaldo Cruz

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aug 2013, Volume 108 N. 5 Pages 548 - 553

Abstract: En | Text: En | PDF: En

Asymptomatic influenza virus infections in pigs are frequent and the lack of measures for controlling viral spread facilitates the circulation of different virus strains between pigs. The goal of this study was to demonstrate the circulation of influenza A virus strains among asymptomatic piglets in an abattoir in Brazil and discuss the potential public health impacts. Tracheal samples (n = 330) were collected from asymptomatic animals by a veterinarian that also performed visual lung tissue examinations. No slaughtered animals presented with any noticeable macroscopic signs of influenza infection following examination of lung tissues. Samples were then analysed by reverse transcription-polymerase chain reaction that resulted in the identification of 30 (9%) influenza A positive samples. The presence of asymptomatic pig infections suggested that these animals could facilitate virus dissemination and act as a source of infection for the herd, thereby enabling the emergence of influenza outbreaks associated with significant economic losses. Furthermore, the continuous exposure of the farm and abattoir workers to the virus increases the risk for interspecies transmission. Monitoring measures of swine influenza virus infections and vaccination and monitoring of employees for influenza infection should also be considered. In addition regulatory agencies should consider the public health ramifications regarding the potential zoonotic viral transmission between humans and pigs.

https://doi.org/10.1590/0074-0276108052013003 3564 downloads

Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing
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Mendonça, Marcos César Lima de

; Ferreira, Ana Maria de Amorim

; Santos, Marta Gonçalves Matos dos

; Oviedo, Elva Cristina

; Bello, Maria Sônia Dal

; Siqueira, Marilda Mendonça

; Maceira, Juan Manuel Piñeiro

; von Hubinger, Maria Genoveva

; Couceiro, José Nelson dos Santos Silva

Memórias do Instituto Oswaldo Cruz

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jun 2011, Volume 106 N. 4 Pages 502 - 504

Abstract: En | Text: En | PDF: En

Heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing were utilised to genotype human parvovirus B19 samples from Brazil and Paraguay. Ninety-seven serum samples were collected from individuals presenting with abortion or erythema infectiosum, arthropathies, severe anaemia and transient aplastic crisis; two additional skin samples were collected by biopsy. After the procedure, all clinical samples were classified as genotype 1.

3065 downloads

Fístula traumática entre coronária direita e câmara atrial direita
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Lemos, Alessandra Edna Teófilo

; Araújo, Antonio Luiz Júnior

; Belém, Lucia de Souza

; Mejia, Juan Alberto Cosquilo

; Cruz Júnior, Aluisio

; Evangelista, Nelson Lopes

; Oliveira, Luciana Santos

Arquivos Brasileiros de Cardiologia

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mar 2007, Volume 88 N. 3 Pages e66 - e67

Abstract: Pt | Text: Pt | PDF: Pt

2695 downloads

Modelo de gerenciamento da manutenção de equipamentos de radiologia convencional
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Couto, Nelson Fraga do

; Ribeiro, Rildo Santos

; Azevedo, Ana Cecília Pedrosa de

; Carvalho, Antonio Carlos Pires

Radiologia Brasileira

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dec 2003, Volume 36 N. 6 Pages 353 - 361

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Foi elaborado um modelo de gerenciamento da manutenção dos equipamentos convencionais de raios X no Hospital Universitário Clementino Fraga Filho. O modelo foi implementado em várias etapas, que incluíram visitas a centros hospitalares que gerenciam seus equipamentos, linha de colaboração com a Fundação Oswaldo Cruz para realização de cursos e treinamento de técnicos de eletrônica do hospital, montagem de uma esquemateca dos equipamentos em uso, criação de um programa de gerenciamento utilizando um banco de dados na plataforma Microsoft Windows 98-Access, levantamento operacional e condições de funcionamento dos equipamentos. A criação de uma nova rotina nas manutenções vai ao encontro das necessidades de reduzir o tempo de atendimento, custos, e de melhoria na qualidade da imagem em um serviço de radiodiagnóstico, atendendo também às exigências da Portaria 453/98 do Ministério da Saúde.

We developed a management model for maintenance of hospital radiological X-ray equipment. The model was implemented in several steps including visits to other hospitals where equipment management is also done, collaboration work with "Fundação Oswaldo Cruz" for the realization of courses and a training program for the hospital electronic technicians, organization of a small library with the schemes of the radiological equipment in use at "Hospital Universitário Clementino Fraga Filho", Rio de Janeiro, Brazil, development of a management software using a database from Access (Microsoft Windows) and evaluation of the performance of the radiological equipment. The implementation of a new routine for equipment maintenance fulfils the need for reducing repair delay and costs, improving image quality in radiodiagnosis services, and complying with resolution 453/98 of the Brazilian Ministry of Health.

24514 downloads

Reanastomose Tubária Laparoscópica: Resultados Preliminares
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Ribeiro, Sérgio Conti

; Tormena, Renata Assef

; Bedin, Alessandra de Araújo Silva

; Giribela, Cassiana Rosa Galvão

; Izzo, Carlos Roberto

; Santos, Nelson da Cruz

; Pinotti, José Aristodemo

Revista Brasileira de Ginecologia e Obstetrícia

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jun 2002, Volume 24 N. 5 Pages 337 - 341

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Objetivos: avaliar as taxas de gravidez após realização de reversão laparoscópica de obstrução tubária por laqueadura prévia. Métodos: entre dezembro de 1998 e dezembro de 2001, realizamos estudo prospectivo no qual foram incluídas 26 pacientes previamente submetidas a esterilização tubária. As pacientes tinham idades entre 28 e 37 anos e a reanastomose foi efetuada por via laparoscópica. O período de seguimento variou entre 150 e 425 dias e as taxas de permeabilidade tubária (estudada por meio da histerossalpingografia) e as taxas de gestação foram avaliadas. Resultados: a reversão laparoscópica pôde ser realizada em 23 pacientes, sendo bilateral em 21. Duas pacientes apresentavam uma tuba extremamente danificada e três haviam sido submetidas a fimbriectomia bilateral. O tempo cirúrgico variou de 95 a 155 minutos e a alta hospitalar ocorreu na manhã seguinte em todos os casos. Constatou-se permeabilidade tubária em 15 pacientes (15/23) e a taxa de gestação foi 56,5% (13/23), sem gestações ectópicas. O intervalo entre a interrupção do uso do condom e a gravidez foi de até quatro meses em nove pacientes e de até 10 meses em quatro pacientes. Conclusão: em casos selecionados, a reversão tubária videolaparoscópica pode ser oferecida à pacientes laqueadas que desejam novas gestações. A seleção adequada das pacientes, assim como técnica cirúrgica meticulosa, são os fatores-chave para atingir taxas de gestação satisfatórias.

Purpose: to evaluate pregnancy outcome after laparoscopic tubal anastomosis. Method: from December 1998 to December 2001, 26 patients with bilateral tubal ligation who underwent laparoscopic tubal anastomosis were prospectively evaluated. Patients' age ranged from 28 to 37 years. The follow-up period ranged from 150 to 425 days and tubal patency (evaluated by hysterosalpingography) and pregnancy outcome were evaluated. Results: laparoscopic tubal reversal was performed in 23 patients. Bilateral reversal was attempted in all except two patients. The operation time ranged from 95 to 155 minutes and all patients were discharged on the following morning. Tubal patency was confirmed in 15 patients (15/23). Pregnancy rate was 56.5% (13/23), without ectopic pregnancies. Nine patients got pregnant in up to four months and four in up to nine months after discontinuing the use of condom. Conclusion: in selected cases, laparoscopic tubal reversal can be performed in patients who had been submitted to tubal sterilization and desire new pregnancies. Patient selection as well as meticulous surgical technique are key factors in achieving satisfactory pregnancy rates.

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10.

Tratamento Cirúrgico Conservador da Hidrossalpinge: Laparoscopia ou Microcirurgia?
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Ribeiro, Sérgio Conti

; Silva, Alessandra de Araujo

; Izzo, Carlos Roberto

; Santos, Nelson da Cruz

; Izzo, Vicente Mário

; Pinotti, José Aristodemo

Revista Brasileira de Ginecologia e Obstetrícia

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dec 2001, Volume 23 N. 10 Pages 627 - 631

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Objetivos: analisar as taxas de gravidez após realização de correção laparoscópica e microcirúrgica de hidrossalpinge. Métodos: no período de julho de 1996 a maio de 1999, foram tratadas 39 pacientes com hidrossalpinge, segundo protocolo de pesquisa previamente aprovado. As pacientes foram distribuídas, por sorteio, em dois grupos, de acordo com o tipo de acesso cirúrgico a ser utilizado: salpingostomia laparoscópica ou por laparotomia. Para análise dos resultados, as pacientes foram estratificadas de acordo com o grau de lesão tubária e as taxas de gestação nos dois grupos foram anotadas durante um intervalo de 24 meses. Resultados: as taxas de gravidez foram de 35,3 e 33,3%, respectivamente, após laparoscopia e microcirurgia. Em relação à gravidade da lesão tubária, 66,7% das pacientes com lesões leves e 21,7% das pacientes com lesões moderadas obtiveram sucesso na concepção. As taxas cumulativas de gravidez em um e dois anos, respectivamente, foram de 25,0 e 34,4%. Houve um caso de gestação ectópica, correspondendo a 9,1% de todas as gestações. Conclusões: pacientes com lesão tubária leve ou moderada podem ser tratadas inicialmente por cirurgia e o sucesso na concepção é inversamente proporcional ao grau de acometimento tubário.

Purpose: to analyze the pregnancy rates after laparoscopic and microsurgical treatment of hydrosalpinx. Methods: from July 1996 to May 1999 thirty-nine infertile patients with hydrosalpinx were treated according to a previously approved research protocol. They were randomly divided into two groups, according to the previously proposed surgical approach: laparoscopic or open microsurgical salpingostomy. To analyze the results, patients were stratified according to tubal damage, and pregnancy rates in both groups were determined for 24 months. Results: pregnancy rates in our series were 35.3 and 33.3% after laparoscopic and microsurgical salpingostomy, respectively. According to the severity of tubal damage, patients with mildly and moderately damaged tubes got pregnant in 66.7 and 21.7% of the cases, respectively. Cumulative pregnancy rates in one and two years were 25.0 and 34.4%, respectively. There was a single case of ectopic pregnancy, corresponding to 9.1% of all pregnancies. Conclusion: patients with mild and moderate lesions may be initially treated with surgery, and conception success is inversely proportional to the degree of tubal damage.

28532 downloads Cited 1 time in SciELO

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