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ABSTRACT Chrysanthemum (Dendranthema grandiflora) is the second most produced and commercialized ornamental plant in the world. Measuring leaf area through non-destructive methods is fundamental for studies on its growth and production. The estimation of leaf area by linear dimensions of the leaves can be a strategy for this purpose. The objective of this study was to find allometric equations to estimate the leaf area of chrysanthemum. The linear, linear without intercept, quadratic, cubic, power, and exponential regression models were used for the analysis. The choice of equations was based on the highest coefficients of determination. The non-destructive method using allometric models has accuracy for estimating the leaf area (LA) of chrysanthemum from the product between leaf length (L) and leaf width (W). The LA of chrysanthemum can be estimated using the equation ŷ = 0.6611*LW0.9490 (L - leaf length; W - leaf width). This equation will allow researchers and producers to determine leaf area non-destructively.

RESUMO O crisântemo (Dendranthema grandiflora) é a segunda planta ornamental mais produzida e comercializada no mundo. A medição da área foliar por métodos não destrutivos é fundamental para estudos sobre seu crescimento e produção. A estimativa da área foliar por dimensões lineares das folhas pode ser uma estratégia para este fim. O objetivo deste estudo foi encontrar equações alométricas para estimar a área foliar do crisântemo. Os modelos de regressão linear, linear sem intercepto, quadrático, cúbico, potência e exponencial foram utilizados para a análise. A escolha das equações foi baseada nos maiores coeficientes de determinação. O método não destrutivo por meio de modelos alométricos tem acurácia para estimar a área foliar (AF) do crisântemo a partir do produto entre o comprimento da folha (C) e a largura da folha (L). A AF do crisântemo pode ser estimada pela equação ŷ = 0,6611*CL0,9490 (C - comprimento da folha; L - largura da folha). Essa equação permitirá que pesquisadores e produtores determinem a área foliar de forma não destrutiva.

https://doi.org/10.1590/1807-1929/agriambi.v27n12p934-940

How to measure organizational resilience? Validating a simplified model
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Santos, Alã Y. P.

; Spers, Renata G.

RAM. Revista de Administração Mackenzie

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2023, Volume 24 N. 6 elocation eRAMD230007

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract Purpose: This study sought to analyze the elements that compose the resilience of organizations when facing a disruptive event, using as context the crisis caused by Covid-19. Originality/value: The study of organizational resilience helps us to theorize, more accurately, organizational adjustment and adaptation in increasingly complex and difficult-to-understand environments. However, although their research has shown promise, its concept still needs more independent attention and empirical validation. In this sense, this paper contributes to a holistic view with a consistent definition of the term through a quantitatively validated model since most of the empirical studies found in the literature are qualitative in nature. Design/methodology/approach: A survey was applied to test a model for measuring organizational resilience, through an exploratory factor analysis, in addition to the use of simple descriptive statistics. Data collection occurred between November 9 and December 23, 2021, through an electronic form with 41 respondents (companies). Findings: The tested model showed a high level of reliability (McDonald’s ω of 0.955) and can be applied within organizations to measure their resilience. The tested model comprises 12 items: unity of purpose; stress testing plans; strategic planning; proactive posture; breaking silos; leveraged knowledge; internal resources; effective partnerships; innovation and creativity; decision-making; staff engagement; and leadership.

Resumo Objetivo: Este estudo buscou analisar os elementos que compõem a resiliência das organizações diante de um evento disruptivo, utilizando como contexto a crise provocada pela Covid-19. Originalidade/valor: O estudo da resiliência organizacional nos ajuda a teorizar com mais precisão o ajuste e a adaptação organizacional em ambientes cada vez mais complexos e difíceis de ser compreendidos. Entretanto, embora a sua investigação tenha demonstrado ser algo promissor, o seu conceito ainda necessita de maior atenção independente e validação empírica. Nesse sentido, este trabalho contribui para que haja uma visão holística com uma definição consistente do termo, por meio de um modelo validado quantitativamente, pois a maioria dos estudos empíricos encontrados na literatura é de natureza qualitativa. Design/metodologia/abordagem: Foi aplicada uma survey a fim de testar um modelo para a mensuração da resiliência organizacional, por meio de uma análise fatorial exploratória, além da utilização de uma estatís-tica descritiva simples. A coleta dos dados ocorreu entre os dias 9 de novembro e 23 de dezembro de 2021, por meio de um formulário eletrônico, que contou com 41 respondentes (empresas). Resultados: O modelo testado apresentou um alto nível de confiabili-dade (ω de McDonald de 0,955), podendo ser aplicado no âmbito das organizações para medir sua resiliência. O modelo testado é composto por 12 itens: unidade de propósito; planos de teste de estresse; planejamento estratégico; postura proativa; quebra de silos; conhecimento aproveitado; recursos internos; parcerias eficazes; inovação e criatividade; tomada de decisão; engajamento da equipe; e liderança.

https://doi.org/10.1590/1678-6971/eramd230007.en

USING REMOTELY PILOTED AIRCRAFT (RPA) IMAGERY TO MAP THE PROFITABILITY OF COTTON CROPS
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Santos, Erli P. dos

; Moreira, Michel C.

; Santana, Charles C.

; Perina, Fabiano J.

; Cruz, Renata R. P.

; Carvalho, Luís G.

Engenharia Agrícola

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2023, Volume 43 N. 3 elocation e20220218

Abstract: En | Text: En | PDF: En

ABSTRACT The goal of this paper was to apply a method for the delineation of vegetation homogeneous zones (HZs) to map the profitability of cotton ( Gossypium sp.) using NDVI (Normalized Difference Vegetation Index) data from RPA imagery. Two irrigated cotton crops, at the FAU (103 ha) and FRP farms (106 ha), located in the western region of Bahia State, Brazil, were studied. The NDVI images were classified into three HZs: “high”, “medium” and “low” plant vigor; using the k-means clustering method. In each HZ the yield was measured, and the profitability estimated. In FAU we found that the lower the HZ’s NDVI values, the lower its profitability, because there was profit in the “high vigor” HZ, of US$ 829.40 ha-1, and loss in the “low vigor” HZ, of about US$ ‒1.256.09 ha-1 (‒251.44% compared with the “high vigor”). In the FRP plantation, the lower the NDVI values, the lower the loss of the plantation, as there were losses in all HZs: from “high” to “low vigor” HZ, the percentage difference in profitability was ‒343.43%. Thus, the use of a low-cost modified near-infrared Canon S100 on an RPA enabled the mapping of crop profitability, aiding the search for the factors behind yield variability.

https://doi.org/10.1590/1809-4430-eng.agric.v43n3e20220218/2023

Mammals in São Paulo State: diversity, distribution, ecology, and conservation
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Galetti, Mauro

; Carmignotto, Ana Paula

; Percequillo, Alexandre R.

; Santos, Marcos C. de O.

; Ferraz, Katia Maria P. M. de Barros

; Lima, Fernando

; Vancine, Maurício H.

; Muylaert, Renata L.

; Bonfim, Fernando César Gonçalves

; Magioli, Marcelo

; Abra, Fernanda D.

; Chiarello, Adriano G.

; Duarte, José Maurício Barbanti

; Morato, Ronaldo

; de Mello Beisiegel, Beatriz

; Olmos, Fábio

; Galetti Jr., Pedro Manoel

; Ribeiro, Milton Cezar

Biota Neotropica

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2022, Volume 22 N. spe elocation e20221363

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Os mamíferos são organismos carismáticos que desempenham um papel fundamental na função ecológica e nos serviços ecossistêmicos, como polinização, dispersão de sementes, ciclagem de nutrientes e controle de pragas. O Estado de São Paulo representa apenas 3% do território brasileiro, mas detém 33% da diversidade de mamíferos. A maior parte de seu território é dominado pela agricultura, pastagens e áreas urbanas que afetam diretamente a diversidade e a persistência dos mamíferos na paisagem. Além disso, São Paulo possui o maior porto da América Latina e o maior reservatório de petróleo costeiro do Brasil, com 600 km de extensão de litoral com diversas espécies de mamíferos marinhos. Essas infraestruturas afetam a diversidade, distribuição, ecologia e o futuro dos mamíferos no estado. Aqui, respondemos cinco perguntas principais: 1) Qual é a diversidade de mamíferos silvestres no Estado de São Paulo? 2) Onde eles ocorrem? 3) Qual é o seu impacto positivo e negativo no bem-estar humano? 4) Como os mamíferos persistem em paisagens modificadas pelo homem? 5) Qual é o futuro dos mamíferos no estado? O estado de São Paulo possui 255 espécies de mamíferos nativos, com quatro espécies endêmicas, duas delas globalmente ameaçadas de extinção. Pelo menos seis espécies (dois marsupiais, ariranha, veado-campeiro, veado-cambuta e tatu-canastra) foram extirpadas do estado devido à caça e perda de habitat. O intenso uso humano da terra no estado forçou muitas espécies de mamíferos a mudar sua dieta para lidar com a intensa fragmentação e agricultura. A monocultura em larga escala facilitou a invasão de espécies exóticas, como porcos selvagens (javaporco) e a lebre europeia. Várias espécies de áreas abertas estão expandindo suas áreas de distribuição (lobo-guará, veado-catingueiro) sobre áreas desmatadas e provavelmente refletem mudanças em direção a um clima mais seco. Como o estado possui o maior sistema rodoviário do Brasil, cerca de 40 mil mamíferos de 33 espécies são mortos por ano em colisões, causando um prejuízo econômico de 12 milhões de dólares/ano. A diversidade de mamíferos está concentrada nos maiores remanescentes florestais da Serra do Mar e no interior do Estado, principalmente nas regiões de Ribeirão Preto e Jundiaí. As lacunas amostrais estão concentradas em todo o interior do estado, principalmente na região noroeste. Os mamíferos silvestres desempenham um papel fundamental em muitos serviços ecossistêmicos, mas também podem ser uma preocupação em trazer novas doenças emergentes para as populações humanas. Embora a taxonomia de mamíferos pareça ser bem conhecida, mostramos que novas espécies estão sendo continuamente descobertas no estado. Portanto, pesquisas usando tecnologias tradicionais e novas (eDNA, iDNA, drones), monitoramento populacional de longo prazo, a investigação da interface do conflito homem-vida selvagem e a compreensão do papel único no ecossistema desempenhado pelos mamíferos são um caminho futuro para promover uma paisagem verde sustentável aliada ao bem-estar humano no estado. O plantio de corredores florestais ou de cerrado, principalmente junto aos principais sistemas fluviais, no planalto, o controle da caça ilegal nas áreas costeiras, o manejo dos regimes de fogo no Cerrado e a mitigação dos atropelamentos devem ser uma prioridade para proteger essa notável diversidade de mamíferos.

Abstract Mammals are charismatic organisms that play a fundamental role in ecological functions and ecosystem services, such as pollination, seed dispersal, nutrient cycling, and pest control. The state of São Paulo represents only 3% of the Brazilian territory but holds 33% of its mammalian diversity. Most of its territory is dominated by agriculture, pastures, and urban areas which directly affect the diversity and persistence of mammals in the landscape. In addition, São Paulo has the largest port in Latin America and the largest offshore oil reservoir in Brazil, with a 600 km stretch of coastline with several marine mammal species. These human-made infrastructures affect the diversity, distribution, ecology, and the future of mammals in the state. Here, we answer five main questions: 1) What is the diversity of wild mammals in São Paulo state? 2) Where are they? 3) What is their positive and negative impact on human well-being? 4) How do mammals thrive in human-modified landscapes? 5) What is the future of mammals in the state? The state of São Paulo holds 255 species of native mammals, with four endemic species, two of them globally endangered. At least six species (two marsupials, Giant otter, Pampas deer, Brazilian dwarf brocket deer, and Giant armadillo) were extirpated from the state due to hunting and habitat loss. The intense human land use in the state forced many mammalian species to change their diet to cope with the intense fragmentation and agriculture. Large-scale monoculture has facilitated the invasion of exotic species such as wild boars (javali) and the European hare. Several “savanna-dwelling” species are expanding their ranges (Maned wolf, Brocket deer) over deforested areas and probably reflect changes towards a drier climate. Because the state has the largest road system, about 40,000 mammals from 33 species are killed per year in collisions causing an economic loss of 12 million dollars/year. The diversity of mammals is concentrated in the largest forest remnants of Serra do Mar and in the interior of the State, mainly in the regions of Ribeirão Preto and Jundiaí. Sampling gaps are concentrated throughout the interior of the state, particularly in the northwest region. Wild mammals play a fundamental role in many ecosystem services, but they can also be a concern in bringing new emergent diseases to humans. Although the taxonomy of mammals seems to be well known, we show that new species are continuously being discovered in the state. Therefore, continuous surveys using traditional and new technologies (eDNA, iDNA, drones), long-term population monitoring, investigation of the interface of human-wildlife conflict, and understanding of the unique ecosystem role played by mammals are future avenues for promoting sustainable green landscapes allied to human well-being in the state. The planting of forest or savanna corridors, particularly along with major river systems, in the plateau, controlling illegal hunting in the coastal areas, managing fire regimes in the Cerrado, and mitigating roadkill must be prioritized to protect this outstanding mammal diversity.

https://doi.org/10.1590/1676-0611-bn-2022-1363

Schools reopening and the COVID-19 pandemic: a case study from Macaé, Rio de Janeiro, Brazil
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POZZOBON, ALLAN P. B.

; PETRY, ANA C.

; ZILBERBERG, CARLA

; BARROS, CINTIA M. DE

; NEPOMUCENO-SILVA, JOSÉ L.

; FEITOSA, NATÁLIA M.

; GOMES NETO, LUPIS R.

; RODRIGUES, BRUNO C.

; BRINDEIRO, RODRIGO M.

; NOCCHI, KEITY JAQUELINE C.V.

; MURY, FLAVIA B.

; SOUZA-MENEZES, JACKSON DE

; SILVA, MANUELA L. DA

; MEDEIROS, MARCIO JOSÉ DE

; GESTINARI, RAQUEL S.

; ALVARENGA, ALESSANDRA S. DE

; SILVA, CARINA A.O.

; SANTOS, DANIELE G. DOS

; SILVESTRE, DIEGO HENRIQUE

; SOUSA, GRAZIELE F. DE

; ALMEIDA, JANIMAYRI F. DE

; SILVA, JHENIFER N. DA

; BRANDÃO, LAYZA M.

; DRUMMOND, LEANDRO O.

; CARPES, RAPHAEL M.

; SANTOS, RENATA C. DOS

; PORTAL, TAYNAN M.

; TANURI, AMILCAR

; NUNES-DA-FONSECA, RODRIGO

Anais da Academia Brasileira de Ciências

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2022, Volume 94 elocation e20211361

Abstract: En | Text: En | PDF: En

Abstract Since the first reported case of COVID-19 in Brazil, the public and private educational system started to close. Up to November 2020, scientific discussions about the return of schooling activities have been rarely performed by the national scientific community and police-makers. The great delay of school returning in Brazil contrasts with successful international strategies of school reopening worldwide and seems counterintuitive with the reopening of non-essential activities. Here, important issues to be considered before and during school reopening are reviewed and discussed. COVID-19 testing is essential to avoid disease spreading, but high cost of individual RT-qPCRs impairs an extensive testing strategy for school returning. To reduce costs and increase the speed of diagnosis, we tested the efficiency of a pooled-sample PCR strategy in a cohort of the educational staff in the city of Macaé/RJ, finding five asymptomatic individuals (0,66%) among the 754 people tested. Thus, a polled-sample PCR testing strategy of the educational staff might prevent infection spreading in schools at a reasonable cost. We discuss how our test strategy could be coupled with internationally recognized safety rules to allow for a safe school return and how countries from different world regions are dealing with educational activities during COVID-19 pandemic.

https://doi.org/10.1590/0001-3765202220211361

Self-made home: how and where does the anuran Rhinella dorbignyi build its retreat sites
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Schuck, Laura K.

; Moser, Camila F.

; Farina, Renata K.

; Santos, Nathália L. P. S. dos

; Tozetti, Alexandro M.

Iheringia. Série Zoologia

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2022, Volume 112 elocation e2022021

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Com as próprias patas: Como e onde o Sapinho-de-Jardim Rhinella dorbignyi constrói seus abrigos. Neste estudo, observamos que tocas de Rhinella dorbignyi (Duméril & Bibron, 1841) são distribuídas de uma forma não-aleatória no habitat, sugerindo que esta espécie seleciona sítios para cavá-las. Esta conclusão foi baseada em uma caracterização de 36 tocas e do micro-habitat que as cerca. Estabelecemos um quadrante de 1 m x 1 m com a toca como seu ponto central (n=36) para medir a porcentagem (densidade) e a altura média de gramíneas, plantas herbáceas e arbustos. Todas as medidas foram repetidas em dois quadrantes não utilizados (sem tocas), para avaliar o micro-habitat disponível ao anfíbio (n=72). As tocas são construídas em áreas específicas do habitat com maior porcentagem de gramíneas, ervas mais altas, menos arbustos presentes e pouca área sombreada. Baseado em modelos tridimensionais do interior das tocas (n=15), observamos que todas elas são construídas com uma abertura elíptica que se abre para um canal estreito, perpendicular ao chão. Os canais têm diâmetro máximo médio de 38 mm e diâmetro mínimo médio de 18 mm. O comprimento médio das tocas é de 182 mm, e o volume médio é de 95 mL.

ABSTRACT In this study, we observed that burrows of Rhinella dorbignyi (Duméril & Bibron, 1841) are distributed in a non-random manner in the habitat, suggesting a microhabitat selection for digging. This conclusion was based on a characterization of 36 burrows and surrounding micro-habitat. We established a 1 m x 1 m quadrat with the burrow in its central point (n=36) to measure the percentage (density) and the average heights of grasses, herbs, and shrubs. All measurements were repeated in two unused quadrats (without burrows) to evaluate the available microhabitat (n=72). The burrows are built in specific areas of the habitat with a higher percentage of grass, taller herbs, lower density of shrubs and low shaded sites than the founded at control sites. Based on three-dimensional models of the interior of the burrow (n=15), we observed that all of them were constructed with an elliptical opening that opens into a narrow channel perpendicular to the ground. Channels had a mean maximum diameter of 38 mm and a mean minimum diameter of 18 mm. The mean length of the burrows is 182 mm, and the mean volume is 95 mL.

https://doi.org/10.1590/1678-4766e2022021

Soil organic carbon in no-tillage systems of different ages in Southwest Mato Grosso, Brazil
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Severiano, Renata M.

; Pierangeli, Maria A. P.

; Santos, Nilton de S.

; Xavier, Vinícius

Revista Brasileira de Engenharia Agrícola e Ambiental

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apr 2021, Volume 25 N. 4 Pages 250 - 255

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivou-se neste estudo avaliar o efeito do plantio direto sobre a densidade do solo, o teor de carbono orgânico do solo e o estoque de carbono em Plintossolo Pétrico e Latossolo Vermelho-Amarelo (Oxisols), localizados no município de Pontes e Lacerda, no Estado de Mato Grosso, Brasil. Os tratamentos foram vegetação nativa e plantio direto de 3, 8, 10 e 12 anos. Para as análises de carbono orgânico do solo amostras de solo foram coletadas em cada área, com três repetições, nas camadas de 0-0,05; 0,05-0,10; 0,10-0,20; 0,20-0,40; 0,40-0,60; 0,60-1,00; 1,00-1,50 e 1,50-2,00 m. Para a densidade do solo foram coletadas amostras indeformadas em 0-0,20 e 0,20-0,40 m. Comparando-se com a vegetação nativa, os valores da densidade do solo aos 12 anos aumentaram 25% no Latossolo Vermelho-Amarelo e 30% no Plintossolo. No Latossolo e Plintossolo, respectivamente, os teores de carbono orgânico foram, em média, 20,57; 25,04 g kg-1 na vegetação nativa; 16,82; 16,59 g kg-1 aos 3 anos de plantio direto; 13,31; 4,96 g kg-1 aos 8 anos; 16,52; 14,39 g kg-1 aos 10 anos; e 17,97; 18,53 g kg-1 aos12 anos. Em ambos os solos o plantio direto vem contribuindo para o aumento dos estoques de carbono ao longo dos anos, porém não em profundidade, limitando-se em geral até 0,20 m. Comparados à vegetação nativa, aos 12 anos de plantio direto houve diminuição dos estoques de carbono a uma taxa de 0,075 Mg ha-1 ano-1 no Plintossolo e um aumento de 2,3 Mg ha-1 ano-1 no Latossolo.

ABSTRACT The objectives of this study were to evaluate the effect of the no-tillage system on soil bulk density, soil organic carbon, and carbon stocks in Plinthic subgroups and Oxisols, located in Pontes and Lacerda, State of Mato Grosso, Brazil. The treatments were native vegetation and no-tillage systems established for 3, 8, 10, and 12 years. To analyse soil organic carbon, soils were sampled in each area, with three repetitions, at layers of 0-0.05; 0.05-0.10; 0.10-0.20; 0.20-0.40; 0.40-0.60; 0.60-1.00; 1.00-1.50 and 1.50-2.00 m. For soil bulk density, undisturbed samples were collected at layers of 0-0.20 and 0.20-0.40 m. Compared with areas of native vegetation, soil bulk density values after 12 years increased by 25% in Oxisols and 30% in the Plinthic subgroups. In Oxisols and Plinthic subgroups, respectively, organic carbon concentration was, on average, 20.57, 25.04 g kg-1 under native vegetation; 16.82, 16.59 g kg-1 after 3 years of no-tillage; 13.31, 4.96 g kg-1 after 8 years; 16.52, 14.39 g kg-1 after 10 years; and 17.97, 18.53 g kg-1 after 12 years. In both soils, the no-tillage system contributed to an increase in carbon stocks over the years, but not at depth, being generally limited to the top 0.20 m of the soils. Compared to native vegetation, after 12 years of no-tillage, carbon stocks decreased at a rate of 0.075 Mg ha-1 year-1 in the Plinthic subgroups and increased by 2.3 Mg ha-1 year-1 in Oxisols.

https://doi.org/10.1590/1807-1929/agriambi.v25n4p250-255 326 downloads

DOES MOISTURE IN PODS INTERFERE WITH MECHANIZED HARVESTING OF PEANUTS?
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Santos, Adão F. dos

; Alcântara, Aline S.

; Corrêa, Lígia N.

; Queiroz, Renata F. de

; Silva, Rouverson P. da

Engenharia Agrícola

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feb 2021, Volume 41 N. 1 Pages 98 - 106

Abstract: En | Text: En | PDF: En

ABSTRACT Although peanut harvesting results in significant losses, it has rarely been investigated. Thus, this study aimed to analyze the interference of different pod moisture contents on peanut harvesting and their influence on the process quality based on losses and impurities. An experiment was performed in a peanut seed producing area, in Luzitânia, district of Jaboticabal - SP, using average moisture contents of 15% and 20% in the pods; 16 and 14 data points were collected for the 20% and 15% treatments, respectively. The experimental design used statistical process control (SPC) to analyze the collected variables. The results indicated higher losses in the platform than in the machine. In addition, the harvest quality was not affected by the pod moisture content. However, the correct adjustment of machines can restrict total losses to approximately 1% of the yield. Pods with moisture contents of 15% and 20% can be used for peanut harvesting. However, although a pod moisture content of 15% reduced the amount of mineral and vegetal impurities inside the bulk tank, the number of open pods increased, whereas a pod moisture content of 20% provided a greater number of whole pods.

https://doi.org/10.1590/1809-4430-eng.agric.v41n1p98-106/2021 331 downloads

Dehydrobufotenin extracted from the Amazonian toad Rhinella marina (Anura: Bufonidae) as a prototype molecule for the development of antiplasmodial drugs
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Banfi, Felipe Finger

; Krombauer, Gabriela Camila

; Fonseca, Amanda Luisa da

; Nunes, Renata Rachide

; Andrade, Silmara Nunes

; Rezende, Millena Alves de

; Chaves, Mariana Helena

; Monção Filho, Evaldo dos Santos

; Taranto, Alex Guterres

; Rodrigues, Domingos de Jesus

; Vieira Júnior, Gerardo Magela

; Castro, Whocely Victor de

; Varotti, Fernando de Pilla

; Sanchez, Bruno Antonio Marinho

Journal of Venomous Animals and Toxins including Tropical Diseases

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2021, Volume 27 elocation e20200073

Abstract: En | Text: En | PDF: En

Abstract Background: The resistance against antimalarial drugs represents a global challenge in the fight and control of malaria. The Brazilian biodiversity can be an important tool for research and development of new medicinal products. In this context, toxinology is a multidisciplinary approach on the development of new drugs, including the isolation, purification, and evaluation of the pharmacological activities of natural toxins. The present study aimed to evaluate the cytotoxicity, as well as the antimalarial activity in silico and in vitro of four compounds isolated from Rhinella marina venom as potential oral drug prototypes. Methods: Four compounds were challenged against 35 target proteins from P. falciparum and screened to evaluate their physicochemical properties using docking assay in Brazilian Malaria Molecular Targets (BraMMT) software and in silico assay in OCTOPUS® software. The in vitro antimalarial activity of the compounds against the 3D7 Plasmodium falciparum clones were assessed using the SYBR Green I based assay (IC50). For the cytotoxic tests, the LD50 was determined in human pulmonary fibroblast cell line using the [3(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) assay. Results: All compounds presented a ligand-receptor interaction with ten Plasmodium falciparum-related protein targets, as well as antimalarial activity against chloroquine resistant strain (IC50 = 3.44 μM to 19.11 μM). Three of them (dehydrobufotenine, marinobufagin, and bufalin) showed adequate conditions for oral drug prototypes, with satisfactory prediction of absorption, permeability, and absence of toxicity. In the cell viability assay, only dehydrobufotenin was selective for the parasite. Conclusions: Dehydrobufotenin revealed to be a potential oral drug prototype presenting adequate antimalarial activity and absence of cytotoxicity, therefore should be subjected to further studies.

https://doi.org/10.1590/1678-9199-jvatitd-2020-0073 992 downloads

10.

SALICYLIC ACID INCORPORATION IN Fe3O4-BSA NANOPARTICLES FOR DRUG RELEASE
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Neves, Renata P.

; Bronze-Uhle, Erika S.

; Santos, Pâmela L.

; Lisboa-Filho, Paulo N.

; Magdalena, Aroldo G.

Química Nova

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2021, Volume 44 N. 7 Pages 824 - 829

Abstract: En | Text: En | PDF: En

The controlled release of Salicylic Acid (SA) influences the concentration and collateral effects of the drug. This release refers to the matrix in which the SA is incorporated. Among the matrices, Fe3O4 nanoparticles (NPs) stand out, for transporting drugs to specific sites. The functionalization of Fe3O4 by bovine serum albumin (BSA) can improve colloidal and chemical stability, in addition to increasing interactions with drugs. Thus, understanding the release kinetics of the AS incorporated in Fe3O4-BSA is essential to improve the controlled release. The study aimed the synthesis, characterization and release of the SA into the Fe3O4-BSA NPs. The results showed the functionalization of the Fe3O4-BSA NPs was effective and the average size was below 30 nm. The NPs showed colloidal stability above the pH of 7.5 which can be used as a drug carrier in blood plasma. Drug encapsulation into the NPs system was efficient (~91%) with about 30% of drug loading capability. The kinetic results showed the SA release mechanism was controlled by diffusion. The conclusion is that the incorporation of SA in Fe3O4-BSA NPs led to a release of SA in the first six hours, reaching equilibrium at 0.265 mg mL-1; and 1.83 mg.

https://doi.org/10.21577/0100-4042.20170733 22 downloads

11.

Purification and characterization of fibrinolytic protease from Streptomyces parvulus by polyethylene glycol-phosphate aqueous two-phase system
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ALENCAR, VIVIANE N.S.

; NASCIMENTO, MARIA CLARA DO

; FERREIRA, JULYANNE V. DOS SANTOS

; BATISTA, JUANIZE M. DA SILVA

; CUNHA, MARCIA N.C. DA

; NASCIMENTO, JÉSSICA M. DO

; SOBRAL, RENATA V. DA SILVA

; COUTO, MILENA T.T. DO

; NASCIMENTO, THIAGO P.

; COSTA, ROMERO M.P.B.

; PORTO, ANA LÚCIA F.

; LEITE, ANA CRISTINA L.

Anais da Academia Brasileira de Ciências

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2021, Volume 93 elocation e20210335

Abstract: En | Text: En | PDF: En

Abstract Fibrinolytic proteases are a promising alternative in the pharmaceutical industry, they are used in the treatment of cardiovascular diseases, especially thrombosis. Microorganisms are the most interesting source of fibrinolytic proteases. The aim of this study was the production of fibrinolytic protease from Streptomyces parvulus DPUA 1573, the recovery of the protease by aqueous two-phase system and partial biochemical characterization of the enzyme. The aqueous two-phase system was performed according to a 24-full factorial design using polyethylene glycol molar mass, polyethylene glycol concentration, citrate concentration and pH as independent variables. It was analyzed the effect of different ions, surfactants, inhibitors, pH and temperature on enzyme activity. The best conditions for purifying the enzyme were 17.5% polyethylene glycol 8,000, 15% Phosphate and pH 8.0, it was obtained a partition coefficient of 7.33, a yield of 57.49% and a purification factor of 2.10-fold. There was an increase in enzyme activity in the presence of Fe2+ and a decrease in the presence of $\beta$-Mercaptoethanol, phenylmethylsulfonyl fluoride and Iodoacetic acid. The optimum pH was 7.0 and the optimum temperature was 40 ºC. The purified protease exhibited a molecular mass of 41 kDa. The fibrinolytic protease from Streptomyces parvulus proved to be a viable option for the development of a possible drug with fibrinolytic action.

https://doi.org/10.1590/0001-3765202120210335 1 downloads

12.

Poor Sleep quality and health-related quality of life impact in adolescents with and without chronic immunosuppressive conditions during COVID-19 quarantine
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Helito, Alberto C.

; Lindoso, Livia

; Sieczkowska, Sofia M.

; Astley, Camilla

; Queiroz, Ligia B.

; Rose, Natalia

; Santos, Claudia Renata P.

; Bolzan, Thalis

; Peralta, Rita María I.A.

; Franco, Ruth R.

; Cominato, Louise

; Pereira, Rosa Maria R.

; Tannuri, Uenis

; Campos, Lucia Maria A.

; Lourenço, Benito

; Toma, Ricardo K.

; Medeiros, Karina

; Watanabe, Andréia

; Grangeiro, Patricia Moreno

; Farhat, Sylvia C.

; Casella, Caio B.

; Polanczyk, Guilherme V.

; Gualano, Bruno

; Silva, Clovis A.

; Sallum, Adriana M. E.

; Iraha, Amanda Y.

; Ihara, Bianca P.

; Mazzolani, Bruna C.

; Martinez, Claudia A.

; Strabelli, Claudia A. A.

; Fonseca, Claudia B.

; Lima, Dandara C. C.

; Setoue, Debora N. D.

; Roz, Deborah F. P.

; Smaira, Fabiana I.

; Roschel, Hamilton

; Miyatani, Helena T.

; Marques, Isabela G.

; Oba, Jane

; Ferreira, Juliana C. O.

; Simon, Juliana R.

; Kozu, Katia

; Saccani, Ligia P.

; Martiniano, Lorena V. M.

; Miranda, Luana C. A.

; Silva, Luiz E. V.

; Laurentino, Moisés F.

; Aikawa, Nadia E.

; Sakita, Neusa K.

; Tanigava, Nicolas Y.

; Pereira, Paulo R. A.

; Palmeira, Patrícia

; Angelo, Simone S.

; Lavorato, Sofia S. M.

; Bernardes, Tamires M.

; Franco, Tathiane C.

; Viana, Vivianne S. L.

; Barros, Vera P. M. F. R.

; Zheng, Yingying

Clinics

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2021, Volume 76 elocation e3501

Abstract: En | Text: En | PDF: En

OBJECTIVE: To assess the possible factors that influence sleep quality in adolescents with and without chronic immunosuppressive conditions quarantined during the coronavirus disease 2019 (COVID-19) pandemic. METHODS: This cross-sectional study included 305 adolescents with chronic immunocompromised conditions and 82 healthy adolescents. Online surveys were completed, which included questions on socio-demographic data and self-rated healthcare routine during COVID-19 quarantine and the following validated questionnaires: the Pittsburgh Sleep Quality Index (PSQI), Pediatric Quality of Life Inventory 4.0 (PedsQL4.0), and Pediatric Outcome Data Collection Instrument (PODCI). RESULTS: The median current age [14 (10-18) vs. 15 (10-18) years, p=0.847] and frequency of female sex (62% vs. 58%, p=0.571) were similar in adolescents with chronic conditions compared with healthy adolescents. The frequency of poor sleep quality was similar in both groups (38% vs. 48%, p=0.118). Logistic regression analysis, including both healthy adolescents and adolescents with chronic conditions (n=387), demonstrated that self-reported increase in screen time (odds ratio [OR] 3.0; 95% confidence interval [CI] 1.3-6.8; p=0.008) and intrafamilial violence report (OR 2.1; 95% CI 1.2-3.5; p=0.008) were independently associated with poor sleep quality in these adolescents. However, the PODCI global function score was associated with a lower OR for poor sleep quality (OR 0.97; 95% CI 0.94-0.99; p=0.001). Further logistic regression, including only adolescents with chronic conditions (n=305), demonstrated that self-reported increase in screen time (OR 3.1; 95% CI 1.4-6.8; p=0.006) and intrafamilial violence report (OR 2.0; 95% CI 1.2-3.4; p=0.011) remained independently associated with poor quality of sleep, whereas a lower PODCI global function score was associated with a lower OR for sleep quality (OR 0.96; 95% CI 0.94-0.98; p<0.001). CONCLUSION: Self-reported increases in screen time and intrafamilial violence report impacted sleep quality in both healthy adolescents and those with chronic conditions. Decreased health-related quality of life was observed in adolescents with poor sleep quality.

https://doi.org/10.6061/clinics/2021/e3501

13.

Pyrimidine Derivatives: QSAR Studies of Larvicidal Activity against Aedes aegypti
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Monte, Zenaide S.

; Navarro, Daniela M. A. F.

; Aguiar, Júlio C. R. O. F.

; Nascimento, Jessica S.

; Scotti, Marcus T.

; Scotti, Luciana

; Barros, Renata P. C.

; Santos, Aline C. S.

; Pereira, Valéria R. A.

; Falcão, Emerson P. S.

; Melo, Sebastião J.

Journal of the Brazilian Chemical Society

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jul 2020, Volume 31 N. 7 Pages 1531 - 1540

Abstract: En | Text: En | PDF: En

The present study investigated the activity of pyrimidine derivatives against Aedes aegypti. Two compounds, 3c and 3d showed excellent larvicide activity. Additionally, quantitative structure-activity relationship (QSAR) models were built using multiple-linear regression and partial least squares with descriptors generated from Dragon and VolSurf+ software, respectively. The best model is obtained with multiple linear regression (MLR), leading to a robust model. Moreover, the QSAR model is validated by means of some internal validation techniques in order to check its reliability, quality and robustness for predicting the larvicidal activity against A. aegypti. The models confirmed that the three-dimensional structure of molecules, steric properties, hydrophobic polar surface area, log partition (logP) and a simple pattern of substituent groups as methyl, methoxy, and succinimide in the pyrimidine derivatives are responsible for the larvicidal activity of the pyrimidine derivatives. Even more, the activity decreases by an electron-withdrawing group in R1 and increases when it is replaced by an aromatic ring activator group. These findings will aid in further studies of new pyrimidine derivatives active against Aedes aegypti.

https://doi.org/10.21577/0103-5053.20200039 283 downloads

14.

Extratos de Lilium sp., Agapanthus sp. e Hydrangea sp.: comportamento como indicadores naturais em diferentes faixas de pH
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Silva, Jhulli M. da

; Martins, Renata de C.

; Bernardi, Fabiele

; Santos, Camila de M. R. dos

; Nicolini, Keller P.

; Nicolini, Jaqueline

Química Nova

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feb 2020, Volume 43 N. 2 Pages 231 - 238

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

This experimental activity proposes the use of lily (Lilium sp.), agapanthus (Agapanthus sp.) and hydrangea (Hydrangea sp.) extracts to investigate the colorimetric profile of each extract between pH 1-14. Water, ethanol and ethanol/HCl 1% (w/w) were used as extractive solvents. Among the flowers tested, the extracts of orange lily and burnt yellow lily were the ones that presented the greatest color variation with the solution pH change. In addition, the total content of anthocyanins in relation to cyanidin-3-glycosidic was determined and in ascending order we have: yellow lily (LAM: 0,07 ± 0,03) < agapanthus white (AGB: 0,21 ± 0,03) < agapanthus violet (AGV: 1,72 ± 0,13) < blue hydrangea (HAZ: 1,83 ± 0,47) < orange lily (LAL: 2,87 ± 0,52) < burnt yellow lily (LAQ: 9,55 ± 0,46). For this determination methanol/HCl 1% (w/w) was used as the solvent. Using UV-Vis spectroscopy, it is suggested that the behaviors are due to the presence of cyanidin in AGB, AGV, LAL and LAQ, delphinidin in HAZ and apigeninidine in LAM. This activity can be applied in courses that have Chemistry in their curricular matrix and in General Chemistry subjects when the acid-base theme is approached.

https://doi.org/10.21577/0100-4042.20170459 524 downloads

15.

INFLUENCE OF GLYPHOSATE CONCENTRATIONS ON SPRAY SOLUTION PHYSICOCHEMICAL CHARACTERISTICS AND DRIFT POTENTIAL
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Almeida, Dieimisson P.

; Ferreira, Marcelo da C.

; Santos, Renata T. da S.

; Griesang, Fabiano

; Santos, Eduardo de S. dos

; Timossi, Paulo C.

Engenharia Agrícola

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feb 2020, Volume 40 N. 1 Pages 69 - 77

Abstract: En | Text: En | PDF: En

ABSTRACT The herbicide glyphosate is sprayed with varying application volumes that result in different concentrations used in the spray solutions. Thus, this study aimed to evaluate the effect of glyphosate concentrations on the potential risk of droplet drift, surface tension of spray solution, and contact angle of droplets on surfaces. Three experiments were conducted in a completely randomized design. Drift potential was evaluated by median volumetric diameter (MVD), percentage volume of droplets with diameter smaller than 100 μm (%V<100 μm), and coefficient of uniformity (Span), with 12 replications. The surface tension of spray solutions and contact angle of droplets were evaluated on Urochloa ruziziensis leaves, paraffin film, and glass, with four replications. Changing glyphosate concentrations in the spray solution changes the droplet spectrum and contact angle on different surfaces due to changes in spray solution physicochemical characteristics, such as surface tension. The increased glyphosate concentrations in the spray solution lead to increased drift potential of droplets, reducing the surface tension and contact angle of droplets on surfaces.

https://doi.org/10.1590/1809-4430-eng.agric.v40n1p69-77/2020 723 downloads

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