Composite films are an alternative in the replacement of synthetic polymers. These films can be prepared from polysaccharides and used to store various drugs to be applied in different areas. In addition, aminoethoxyvinylglycine (AVG) is an ethylene inhibitor that prolongs shelf life of food. For this reason, this research aimed to take advantage of the brown algae Macrocystis pyrifera and Lessonia trabeculata to extract sodium alginate and produce films composed of sodium alginate/kappa-carrageenan/iota-carrageenan, which were plasticized with glycerol and polyethylene glycol 400 and loaded with aminoethoxyvinylglycine. The extracted sodium alginate was characterized by 1H and 13C nuclear magnetic resonance (NMR), size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS), thermogravimetry (TG/DTG), Fourier transform infrared (FTIR), and X-ray diffraction (XRD); and the composite films were characterized by FTIR, XRD, TG/DTG, and scanning electron microscopy (SEM). Then, the drug release kinetics were investigated using Higuchi and Korsmeyer-Peppas kinetic models. The extracted alginates obtained were of low molecular weight, and the films showed desirable properties for AVG release. Furthermore, drug release profiles revealed that AVG release is governed by Fick’s Law, and this is favored at low temperatures. In summary, sodium alginate allows the preparation of composite films, which can replace synthetic polymers to be used in the loading and releasing of drugs.

Resumo A herbivoria é uma interação de grande impacto sobre as comunidades de plantas, uma vez que as relações entre herbívoros e plantas são fundamentais para a distribuição e abundância das espécies ao longo do tempo e do espaço. O objetivo deste estudo foi monitorar a taxa de expansão foliar da samambaia arborescente Cyathea phalerata e avaliar o dano causado por herbívoros a folhas de diferentes idades, assim como verificar se este dano está relacionado à temperatura e à precipitação. O estudo foi realizado em um fragmento de Floresta Atlântica subtropical localizado no município de Caraá, na encosta nordeste do estado do Rio Grande do Sul, no sul do Brasil. Nós monitoramos 24 indivíduos maduros de C. phalerata com báculos em uma população de aproximadamente 50 plantas. A taxa de expansão foliar, a porcentagem de folhas danificadas e a taxa de consumo da lâmina foliar por herbivoria foram calculados. Médias mensais de temperatura e precipitação acumulada foram calculadas a partir de dados diários. Báculos de C. phalerata expandiram rapidamente durante o primeiro e o segundo mês após emergência (3,98 cm dia-1; 2,91 cm dia-1, respectivamente). O dano causado por herbivoria foi observado em todas as folhas monitoradas, mas nenhuma das plantas sofreu desfolhação completa. A maior porcentagem (57%) de folhas danificadas foi registrada aos 60 dias de monitoramento, e também a maior taxa de consumo mensal (6,04%) ocorreu em folhas jovens, recém expandidas, enquanto esta taxa permaneceu entre 1,50 e 2,21% em folhas maduras. As taxas mensais de consumo da lâmina foliar e de folhas danificadas mostraram relação positiva e forte entre si e com a temperatura. A rápida expansão foliar observada em C. phalerata pode ser considerada uma estratégia fenológica para reduzir o dano a folhas jovens, abreviando o período de desenvolvimento e acelerando o aumento das defesas em folhas maduras.

Abstract Herbivory is an interaction with great impact on plant communities since relationships between herbivores and plants are fundamental to the distribution and abundance of species over time and space. The aim of this study was to monitor the rate of leaf expansion in the tree fern Cyathea phalerata and evaluate the damage caused by herbivores to leaves of different ages and whether such damage is related to temperature and precipitation. The study was performed in a subtropical Atlantic Forest fragment located in the municipality of Caraá, in the northeast hillside of Rio Grande do Sul state, in southern Brazil. We monitored 24 mature individuals of C. phalerata with croziers in a population of approximately 50 plants. Leaf expansion rate, percentage of damaged leaves and leaf blade consumption rate by herbivory were calculated. Monthly means for temperature and accumulated rainfall were calculated from daily data. Croziers of C. phalerata were found to expand rapidly during the first and second months after emergence (3.98 cm day-1; 2.91 cm day-1, respectively). Damage caused by herbivory was observed in all of the monitored leaves, but none of the plants experienced complete defoliation. The highest percentage (57%) of damaged leaves was recorded at 60 days of monitoring, and also the highest monthly consumption rate of the blade (6.04%) occurred with young, newly-expanded leaves, while this rate remained between 1.50 and 2.21% for mature leaves. Rates of monthly leaf consumption and damaged leaves showed positive and strong relationship with each other and with temperature. The rapid leaf expansion observed for C. phalerata can be considered a phenological strategy to reduce damage to young leaves by shortening the developmental period and accelerating the increase of defenses in mature leaves.

Abstract Introduction: High-volume corticosteroid nasal irrigation is a treatment option in patients with chronic rhinosinusitis. In Brazil, alternatives are used to optimize its cost and popularize its use, such as 1% compounded budesonide drops or betamethasone cream, and it is necessary to study these treatment modalities. Objective: To evaluate the clinical response of nasal irrigation with 1% compounded budesonide drops or betamethasone cream compared to nasal sprays utilized in patients with chronic rhinosinusitis. Methods: This was a retrospective observational study with 257 patients. One hundred and eight patients using corticosteroid nasal irrigation (292 treatment cycles) and 149 using corticosteroid nasal spray (300 treatment cycles) were included. Evaluation of subjective improvement, adverse events, exacerbations, and objective assessments with SNOT-22 and Lund-Kennedy endoscopic score were performed, in addition to sub-analyses related to nasal polyps and previous surgery. Results: Corticosteroid nasal irrigation and corticosteroid nasal spray improved the Lund–Kennedy endoscopic score, with more adverse events in the corticosteroid nasal irrigation group. Previous surgery increased corticosteroid nasal irrigation improvement, with greater subjective improvement and fewer exacerbations. 1% compounded budesonide drops were better than betamethasone cream in the Lund-Kennedy endoscopic score, with fewer adverse events. A 1,000 μg dose of 1% compounded budesonide drops was more effective than 500 μg. Conclusion: Corticosteroid nasal irrigation was effective in improving the Lund-Kennedy endoscopic score in chronic rhinosinusitis, especially in patients with nasal polyps and previous surgery, in addition to promoting a higher rate of subjective improvement and fewer exacerbations than corticosteroid nasal spray, but with more adverse events. 1% compounded budesonide drops improved the Lund-Kennedy endoscopic score with fewer adverse events than betamethasone cream, particularly at higher doses (1000 μg).

ABSTRACT In search for a connection between the formation of stars and the formation of planets, a new semi-automatic spectral analysis method using iSpec was developed for the TIGRE telescope installed in Guanajuato, Mexico. TIGRE is a 1.2m robotic telescope, equipped with an Echelle spectrograph (HEROS), with a resolution R ⋍ 20000. iSpec is a synthetic spectral fitting program for stars that allows to determine in an homogeneous way their fundamental parameters: effective temperature, Teff , surface gravity, log g, metallicities, [M=H] and [Fe=H], and rotational velocity, V sin i. In this first article we test our method by analysing the spectra of 46 stars, hosts of exoplanets, obtained with the TIGRE.

RESUMEN En la búsqueda de una conexión entre la formación estelar y planetaria, se desarrolló un nuevo método semiautomático de análisis espectral estelar usando iSpec, para el telescopio TIGRE, instalado en Guanajuato, México. El TIGRE es un telescopio robótico de 1.2m, el cual está equipado con el espectrógrafo Echelle HEROS, que tiene una resolución R ≃ 20; 000. iSpec es un programa de ajuste espectral sintético para estrellas que permite determinar de manera homogénea sus parámetros fundamentales: temperatura efectiva, Teff, gravedad superficial, log g, metalicidades, [M=H] y [Fe=H], y velocidad de rotación, V sin i. En este artículo, probamos nuestro método, analizando una muestra de 46 estrellas que albergan exoplanetas observadas por el TIGRE.

ABSTRACT A sample of 46 stars, host of exoplanets, is used to search for a connection between their formation process and the formation of the planets rotating around them. Separating our sample into two, stars hosting high-mass exoplanets (HMEs) and low-mass exoplanets (LMEs), we found the former to be more massive and to rotate faster than the latter. We also found the HMEs to have higher orbital angular momentum than the LMEs and to have lost more angular momentum through migration. These results are consistent with the view that the more massive the star and the higher its rotation, the more massive was its protoplanetarys disk and rotation, and the more efficient was the extraction of angular momentum from the planets.

RESUMEN Una muestra de 46 estrellas que albergan exoplanetas se usa para la búsqueda de una conexión entre su proceso de formación y el proceso de formación de los planetas que las orbitan. Separamos nuestra muestra en dos: estrellas que albergan exoplanetas de baja masa (LME) y de alta masa (HME); encontramos que las estrellas con HMEs rotan preferentemente alrededor de estrellas con alta masa y con mayor rotación que las estrellas con LMEs. También encontramos que las HMEs tienen un momento angular orbital más alto que las LMEs y que perdieron más momento angular durante su migración. Nuestros resultados son congruentes con un modelo en el cual las estrellas más masivas con alta rotación forman discos protoplanetarios más masivos que también rotan mas rápido, y que además son más eficientes en disipar el momento angular de sus planetas.

Resumo As florestas ripárias apresentam fatores bióticos e abióticos que influenciam a dissimilaridade ambiental, e que refletem a distribuição específica de samambaias. O objetivo deste trabalho foi avaliar a heterogeneidade ambiental associada às comunidades de samambaias terrícolas em remanescentes de mata ciliar dos campos da metade sul do estado do Rio Grande do Sul (Pampa). Foram amostradas dez parcelas de 5×5 m em cada sítio (AI, AII e AIII), totalizando 30 parcelas. Em cada quadro, foram inventariadas as espécies de samambaia, além de outros parâmetros vegetacionais, microclimáticos e edáficos. Um total de 24 espécies foram identificadas nos três remanescentes de mata ciliar, distribuídas em 19 gêneros e 10 famílias. A Análise de Componentes Principais (PCA) mostrou que as parcelas de AI apresentaram os maiores valores de temperatura e alumínio, enquanto a umidade foi a menor, o que se refletiu na diminuição da riqueza de samambaias. A umidade do solo e a umidade do ar foram as variáveis mais relacionadas às parcelas de AII e AIII, indicando que essas plantas foram favorecidas nesses. A identidade florística característica de cada local sugere que muitas espécies são dependentes de certas condições ambientais locais. Nesse sentido, mesmo em sítios geograficamente próximos, remanescentes de matas ciliares merecem atenção no que se refere a conservação, uma vez que são capazes de abrigar grande diversidade nesta fitofisionomia exclusiva do sul do Brasil. Os resultados deste estudo integrado, com parâmetros vegetacionais, climáticos e edáficos, sugerem, que as samambaias respondem categoricamente à heterogeneidade mostrada pelos ambientes ripários, mesmo quando os fragmentos estão próximos.

Abstract Riparian forests present biotic and abiotic factors that influence environmental dissimilarity, which reflect specific fern distribution. The purpose of this this study was to evaluate the environmental heterogeneity associated to the terrestrial fern communities in riparian forest remnants in the grasslands southern half of Rio Grande do Sul state . We sampled ten 5×5 m plots in each site (AI, AII and AIII), totaling 30 plots. In each plot, fern species were inventoried and vegetative, microclimatic and edaphic parameters were collected. A total of 24 species were surveyed in the three riparian forest remnants, distributed in 19 genera and 10 families. The Principal Components Analysis (PCA) showed that plots of AI had the highest temperature and aluminum values, while humidity was the lowest, which was reflected in the decrease of ferns richness. Soil moisture and air humidity were variables more related to the plots of AII and AII, indicating that these plants were favored in these environments. The floristic identity characteristic of each site suggests that many species are dependent to certain local environmental conditions. In this sense, even in very geographically close sites, riparian remnants deserve conservation attention, since they are capable of harboring high diversity in this grassland phytophysiognomy in southern Brazil. The results of this integrated study, with vegetation, climatic, and edaphic parameters, suggest, as expected, that ferns categorically respond to the heterogeneity shown by riparian environments, even when fragments are geographically very close to each other.

RESUMO Objetivou-se avaliar as alterações fisiológicas de ovinos submetidos à restrição alimentar, expostos à radiação solar e à sombra. Dez borregas mestiças foram divididas igualmente em dois grupos: grupo radiação solar - GRS, animais expostos ao sol durante o dia; e grupo sombra - GS, animais alocados dentro de um galpão à sombra. Nos seis dias iniciais de experimento, os animais foram submetidos à restrição alimentar, com acesso apenas à água, já expostos ao sol ou à sombra. Nos seis dias subsequentes, foi ofertada dieta composta por ração e feno de alfafa (3%PV). Os parâmetros avaliados foram frequência respiratória (FR), temperatura retal (TR), β-hidroxibutirato (BHB), pH urinário, ingestão de matéria seca (IMS) e dados bioclimáticos (temperatura, umidade relativa do ar e índice de temperatura e umidade - ITU). A FR foi o parâmetro em que houve maior diferença significativa entre os grupos (p<0,05). O balanço energético negativo foi constatado em todos os animais (BHB>0,8mmol/L; pH<7,0). Ovelhas que sofrem restrição alimentar durante períodos de alta temperatura e umidade têm menor frequência respiratória e temperatura interna, independentemente de estar na sombra ou pleno sol. Porém, quando normalizado a oferta de alimento as ovelhas em pleno sol têm menor ingestão de matéria seca.

ABSTRACT We aimed to evaluate physiological changes in sheep submitted to food restriction, exposed to the sun or shade. Ten ewe lambs were equally divided into two groups: Sun Radiation Group - GRS, exposed to sun during day hours, and Shade Group - GS, with animals placed inside a barn. In the first six days of experiment, animals were submitted to food restriction, with access to fresh water, already exposed to sun and shade. In the following six days alfalfa hay and concentrate were provided to the animals (3%BW). Parameters assessed were respiratory rate (FR), rectal temperature (TR), β-hydroxybutyrate, urinary pH, dry matter intake (IMS) and climate data (air temperature, humidity, and temperature-humidity index - ITU). The parameter that most differed between groups was FR. All animals presented negative energy balance (BHB>0.8mmol/L; pH<7,0). Ewe lambs submitted to food restriction during periods of high temperature and humidity have lower respiratory rate and intern temperature, independently of being exposed to sun or shade. However, when food was offered animals exposed to sun had lower dry matter intake.

Abstract Background: Garlic cultivation is characterized by low capacity of competition with weed, mainly due to the characteristics of the canopy and it´s slow initial growth that make it difficult to cover the soil. Objective: This work aimed to evaluate the efficiency and selectivity of herbicides applied in the pre-emergence of two garlic cultivars in the edaphoclimatic condition of Santa Catarina State. Methods: Two experiments were carried out in the commercial garlic area with Ito and Chonan cultivars. Both experiments were conducted in randomized block design. The traits for Ito cultivar were without weed control, with weed control, with linuron (810 g ha-1), diuron (1,000 g ha-1), pendimethalin (1600 g ha-1), S-metolachlor (1200 g ha-1), oxyfluorfen (720 g ha-1) or flumioxazin (40 g ha-1). For the experiment with Chonan cultivar the treatment composed by oxadiazon (1000 g ha-1) was included. The analyzed variables were based on weed community control and phytotoxicity, stand, diameter of bulbs and productivity of garlic. Results: The infesting species of the experiment with the cultivar Ito were Stellaria media and Stachys arvensis, whereas for Chonan, only the first was found. The herbicides applied in pre-emergence were efficient in controlling these species until 45 days after application - DAA. The highest injuries at 45 DAA were caused by S-metolachlor, with 33% for Ito cultivar and 10% for Chonan. The best herbicides, taking into account weed control and productivity, were pendimethalin and S-metolachlor, for the Ito cultivar, and pendimethalin, oxyfluorfen, and flumioxazin for the Chonan cultivar. Conclusions: The pendimethalin stood out for presenting excellent weed control and being selective for both garlic cultivars.

Resumo Bordas florestais tipicamente exibem maior luminosidade e menor umidade que o interior florestal, resultando em um gradiente abiótico. Entretanto, o grau de diferença abiótica pode ser afetado a partir do tipo da matriz, influenciando a seleção de espécies. Comparamos a composição florística e a estrutura fitossociológica das comunidades de samambaias e licófitas na borda e interior de três sítios influenciados por diferentes matrizes (campo natural, plantio de Pinus e cultivo de olerícolas). Na região de Floresta com Araucária no Rio Grande do Sul, Brasil, foram sorteadas doze parcelas de 10 × 10 m na borda e no interior de cada sítio, totalizando 72 parcelas para avaliar o contraste fitossociológico, utilizando como parâmetro cobertura e riqueza das espécies por parcela para avaliar esse contraste. Registramos um total de 38 espécies nas áreas estudadas, distribuídas em 15 famílias. Os resultados mostraram que o efeito de borda atua em intensidades distintas nos sítios analisados. Nos sítios com matriz antropizada, a composição foi mais homogênea tanto nas bordas, quanto nos interiores e apresentou menor riqueza, demonstrando impacto mais pronunciado e profundo. Já no sítio com matriz de entorno natural, apesar da borda também apresentar baixa riqueza, o interior foi cerca de 3x mais rico. Sugerimos que os esforços de conservação de samambaias e licófitas em fragmentos com araucária, devem se concentrar em sítios inseridos em campo natural, pois, a conversão destes em plantio de Pinus e cultivo de olerícolas, diminui a diversidade dessas plantas, tanto nas bordas quanto nos interiores da floresta, além de alterar a estrutura fitossociológica levando as comunidades à simplificação.

Abstract Forest edges typically exhibit higher luminosity and lower humidity than the forest interior, resulting in an abiotic gradient. However, the degree of abiotic difference can be affected from the type of the matrix, influencing the selection of species. We compared the floristic and phytosociological structure of understory communities of ferns and lycophytes of the edge and interior of three forest sites influenced by different types of surrounding matrices (natural field, Pinus plantation, and cultivation of crops). In the region of Araucaria Forest, in Rio Grande do Sul, Brazil, twelve 10 × 10 m plots were selected at the edge and interior of each site, totaling 72 plots and to evaluate the phytosociological contrast, using as a parameter coverage and species richness per plot to evaluate this contrast. We recorded a total of 38 species in the studied areas, distributed in 15 families. The results show that the edge effect acts at different intensities in the analyzed sites. In the site with unnatural matrix, the composition was more homogeneous both in the edges and in the interiors and presented lower richness, showing a more pronounced and deep impact. Already in the site with natural matrix surroundings, although the border also presents low richness, the interior was about 3x richer. Based on our results, we concluded that fern conservation efforts should focus on fragments of Araucaria Forest inserted in the natural field, because the conversion of natural field into Pinus planting and cultivation of crops decreases ferns species both in the edges and forest interiors of the studied fragments, besides altering the phytosociological structure leading the communities to simplification.

Resumo Dicksonia sellowiana Hook. (Dicksoniaceae) é alvo de exploração extrativista e encontra-se ameaçada de extinção. Analisamos a estrutura populacional, o padrão de distribuição espacial de D. sellowiana e sua relação com parâmetros ambientais no interior de três fragmentos de Floresta com Araucária, no Rio Grande do Sul (RS), Brasil. Os fragmentos possuem diferentes tamanhos sendo denominados de grande (H1LF) com 246 ha, médio (H2MF) com 57 ha e de pequeno (H3SF) com 5,2 ha. No interior de cada sítio, foi demarcado 1 ha, fracionado em 100 subparcelas (100m2), das quais 20 foram sorteadas. Em cada subparcela foi feita a contagem dos indivíduos, o registro da altura do cáudice e da cobertura (SC) (m2) das folhas, as medições da radiação fotossinteticamente ativa (PAR), grau de abertura do dossel (CO), umidade do solo (SM) e espessura da serapilheira (LT). A temperatura (T) foi medida no interior de cada sítio. Foram amostradas 792 plantas, das quais 551 concentradas no H1LF, 108 no H2MF e 133 no H3SF e em média foram estimados 1320 indivíduos ha-1. Do total dos três fragmentos, 96,9% dos indivíduos estão na primeira classe de altura (até 0,8 m), indicando grande potencial de desenvolvimento das populações. O padrão de distribuição espacial (AI) foi agregado nas três populações e as plantas apresentaram uma cobertura total heterogênea, entre 4,73m2 (H2MF) e 2.223,47m 2 (H1LF). Os maiores valores de SC e de SM foram mais relacionadas com a distribuição dos indivíduos no H1LF enquanto que o contrário foi registrado no H2MF. Os maiores valores de PAR, LT e de CO relacionaram-se com a distribuição de D. sellowiana no H3SF. Além de revelar que a população do H1LF está entre as mais densas já descritas no sul do Brasil, os resultados demonstraram uma significativa distinção estrutural entre as populações dos interiores dos fragmentos com diferentes tamanhos, embora muito próximos geograficamente e inseridos em uma mesma matriz de campo natural.

Abstract Dicksonia sellowiana Hook. (Dicksoniaceae) is target of extractive exploitation and is threatened with extinction. We analyzed the population structure, the spatial distribution pattern of D. sellowiana and its relationship with environmental parameters within three fragments of Araucaria Forest in Rio Grande do Sul, Brazil. The fragments are of different sizes, namely, large (H1LF) with 246 ha, medium (H2MF) with 57 ha and small (H3SF) with 5.2 ha. Within each site, 1 ha was delimited, divided into 100 subplots (100 m2), of which 20 were selected with a draw. In each subplot, counting of the individuals, the registration of the caudice height and the coverage of leaves (SC) (m2), measurements of photosynthetically active radiation (PAR), canopy opening degree (CO), soil moisture (SM) and litter thickness (LT). The temperature (T) was measured inside each site. A total of 792 plants were sampled, of which 551 were concentrated in H1LF, 108 in H2MF and 133 in H3SF. An average of 1320 ha-1 individuals were estimated. Of the total including the three fragments, 96.9% of the individuals are in the first class of height (up to 0.8 m), indicating a great potential of population development. The spatial distribution pattern (AI) was aggregated in the three populations and the plants presented a heterogeneous total coverage, between 4.73 m2 (H2MF) and 2,223.47 m2 (H1LF). The highest values of SC and SM were more related to the distribution of individuals in H1LF whereas the opposite was recorded in H2MF. The highest values of PAR, LT and CO correlated with the distribution of D. sellowiana in H3SF. In addition to revealing that the H1LF population is among the most dense in southern Brazil, the results demonstrated a significant structural distinction between the interior populations of the fragments, in spite of them being located near to one another and being part of the same natural field matrix.

Abstract Abstract: Specimens of Leptogium collected in ten localities from the State of Rio Grande do Sul were studied. Sixteen species were found of 28 records mentioned to the state, which represents around 57% of the Leptogium diversity known for Rio Grande do Sul. Leptogium exaratum is proposed as a new species. Leptogium atlanticum, L. milligranum and L. vesiculosum are reported for the first time to the state. The lectotype of L. javanicum is reported here and detailed descriptions are provided to the examined specimens. An identification key is showed with all the 28 mentioned species of Leptogium. It is the first published taxonomic key for Leptogium from Rio Grande do Sul, the State with the highest diversity of this genus in Brazil.

RESUMO A aplicação de fertilizantes nitrogenados, tanto orgânicos quanto minerais, pode ocasionar perdas de nitrato por lixiviação. Para minimizar este efeito, inibidores enzimáticos são utilizados. Entretanto, são desconhecidos seus efeitos sobre o acúmulo de nutrientes, como o fósforo (P) da biomassa microbiana, que é o mais sensível e dinâmico compartimento de nutrientes do solo. Objetivou-se avaliar a influência da aplicação de inibidor enzimático (IE) sobre o teor de fósforo da biomassa microbiana do solo (Pm), quando aplicado adubo mineral (NPK) ou dejeto líquido de suínos (DLS) no cultivo de milho. O experimento foi instalado em um Cambissolo Húmico em Lages – SC (27° 47’ 08” S 50° 18’ 09” O) na safra de 2012/2013. Os tratamentos foram constituídos de: i) NPK; ii) NPK + IE; iii) DLS; iv) DLS + IE, aplicados antecedendo a semeadura do milho e v) controle (sem aplicação de fertilização e de inibidor enzimático) dispostos em delineamento de blocos casualizados com 4 repetições, em sistema de plantio direto. Amostras de solo foram coletadas da camada 0-0,10 m de profundidade aos 0, 10, 25, 58, 90, 135 e 202 dias após a aplicação dos tratamentos. O teor de Pm não foi afetado pela aplicação de inibidores enzimáticos mas sofreu influência ao longo do ciclo da cultura em resposta ao balanço hídrico e cultivo do milho.

ABSTRACT The application of nitrogen fertilizers, both organic and mineral, can cause nitrate losses by leaching. To minimize this effect, enzyme inhibitors are used. However, the effects on microbial biomass, which is the most sensitive and dynamic compartment of nutrients in the soil, as well as its effect on nutrients such as phosphorus (P) are unknown. This study aimed to evaluate the influence of the enzyme inhibitor (EI) on soil microbial phosphorus (Pm) when mineral fertilizer (NPK) or liquid swine manure (LSM) was applied in corn cultivation. The experiment was installed in a Humic Cambisol in Lages – SC, Brazil (27° 47’ 08” S; 50° 18’ 09” W) in the 2012/2013 season. The treatments consisted of: i) NPK; ii) NPK + EI; iii) LSM; iv) LSM + EI, applied before sowing of corn, and v) control (without application of fertilizer and enzyme inhibitor) arranged in a randomized block design, under no-tillage system. Soil samples were collected from the 0-0.10 m layer at 0, 10, 25, 58, 90, 135 and 202 days after application of the treatments. The Pm content was not affected by the enzyme inhibitor application but was influenced throughout the crop cycle in response to water balance and corn cultivation.

RESUMO O fósforo (P) reage com constituintes químicos e mineralógicos no solo, principalmente em solos tropicais e subtropicais. Por isso, estratégias têm sido utilizadas para diminuir a adsorção de P ao solo, dentre elas, a formação de complexos que poderiam diminuir a adsorção do P ao solo. Dois experimentos foram realizados com o objetivo de avaliar a formação de complexos ácido orgânico-Fe-P em laboratório e a eficiência desses complexos sobre o crescimento de plantas de trigo (T. aestivum), cultivadas em casa de vegetação. No experimento 1 foram selecionados sete ácidos orgânicos (AO) de baixo peso molecular e realizado um protocolo de formação de complexos de ácido orgânico e Fe3+ a pH 4,0. Após a agitação foi adicionado P ao complexo ácido orgânico-Fe para a formação do complexo ternário. O P remanescente foi avaliado para determinar a quantidade de P no complexo. No experimento 2, os três AO que apresentaram a maior quantidade de complexos foram selecionados, juntamente com um tratamento com P solúvel e um controle. O trigo foi cultivado em solo acondicionado em vasos em casa de vegetação por 34 dias. A quantidade de complexos ternários ácido orgânico-Fe-P formados variou 5 a 42% do P adicionado. Porém, quando esses complexos foram adicionados ao solo, nos tratamentos com maior quantidade de complexo formado houve menor crescimento do trigo. Assim, o complexo ternário com ácido orgânico-Fe-P é pouco efetivo no desempenho de plantas de trigo.

ABSTRACT Phosphorus (P) reacts with chemical and mineralogical constituents in the soil, mainly in tropical and subtropical soils. Therefore, strategies have been used to reduce the adsorption of P to the soil, among them, the formation of complexes that could decrease P adsorption to the soil. Two experiments were carried out to evaluate the formation of organic acid-Fe-P complexes in the laboratory and the efficiency of these complexes on the growth of wheat plants (T. aestivum) grown under greenhouse conditions. In experiment 1, seven low-molecular-weight organic acids (OA) were selected and a protocol for the formation of complexes between organic acid and Fe3+ at pH 4.0 was performed. After stirring, P was added to the organic acid-Fe complex to form the ternary complex. The remaining P was evaluated to determine the amount of P in the complex. In experiment 2, the three OAs which had the largest amount of complexes were selected, together with a treatment with soluble P and a control. Wheat was grown in potted soil in a greenhouse for 34 days. The amount of ternary organic acid-Fe-P complexes formed varied from 5 to 42% of the added P. However, when these complexes were added to the soil, in treatments with greater amount of complex formed there was lower growth of wheat. Thus, the ternary complex with organic acid-Fe-P has little effect on the performance of wheat plants.

Resumo O clima é um dos principais fatores que condicionam o comportamento das plantas. A fenologia de Guarea macrophylla Vahl, arvoreta utilizada no reflorestamento de áreas degradadas, foi acompanhada durante 18 meses, em mata ciliar do arroio Schmidt, Campo Bom, RS, Brasil. Foram observados os eventos vegetativos (queda foliar e brotamento) e reprodutivos, separados em floração (botão floral e antese) e frutificação (frutos imaturos, maturando e maduros). Os eventos fenológicos foram relacionados à temperatura, ao fotoperíodo e à precipitação e a sua sazonalidade foi verificada por meio da análise estatística circular. As fenofases vegetativas foram contínuas, não se relacionaram com os fatores climáticos e apresentaram baixa intensidade, ressaltando a característica perene da espécie. A floração ocorreu durante a primavera e o verão e tanto botão floral quanto antese relacionaram-se com temperatura e fotoperíodo. A frutificação foi constante e passou por todos os estádios de desenvolvimento. Os frutos imaturos desenvolveram-se em meses com o menor fotoperíodo e maturaram mais intensamente no inverno, em dias de menor temperatura. Os frutos maduros tornaram-se disponíveis para os dispersores na primavera, em períodos com maior fotoperíodo e temperatura. Com exceção da queda foliar, todos os outros eventos fenológicos apresentaram sazonalidade em sua manifestação. O adiantamento de um mês entre as florações observadas no presente estudo indicou que as alterações climáticas locais de temperatura induziram a ocorrência antecipada dessa fenofase.

Abstract Climate is one of the main factors that affect plant behavior. The phenology of Guarea macrophylla Vahl, which is a small tree used for reforestation of degraded areas, was monitored for 18 months in a riparian forest at the Schmidt Stream, Campo Bom, in the state of Rio Grande do Sul, southern Brazil. Vegetative (leaf fall and leaf flushing) and reproductive events were observed, with the latter divided into flowering (flower buds and anthesis) and fruiting (unripe, ripening and ripe fruit). Phenological events were related to temperature, photoperiod and precipitation and their seasonality was verified by circular statistical analysis. Vegetative phenophases were continuous; they were not related to climate factors and presented low intensity, emphasizing the perennial aspect of the species. Flowering occurred during spring and summer. Both flower buds and anthesis were related to temperature and photoperiod. Fruiting was constant and went through all stages of development. Unripe fruits developed during the months with the lowest photoperiod and ripen more intensely in winter, on colder days. Ripe fruit became available for dispersal in spring, in times of longer photoperiod and higher temperatures. Except for leaf fall, all other phenological events showed seasonality in their manifestation. The one-month difference between the onsets of the flowering phases observed in this study indicated that local climate changes induced the early occurrence of this phenophase.