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au:SCHNEIDER, MARIA PAULA
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1.
Development and characterization of bacterial cellulose membrane incorporated with Witch hazel extract
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Molin, Maria Luiza
; Segat, Bruna
; Garcia, Michele Cristina Formolo
; Pezzin, Ana Paula Testa
; Silveira, Márcia Luciane Lange
; Schneider, Andrea Lima dos Santos
.
ABSTRACT Bacterial cellulose (BC) has stood out in the biomedical field for its biocompatibility, non-toxicity and high liquid absorption capacity. Thus, studies have been conducted aiming at the functionalization of BC with substances that add properties, such as anti-inflammatory and antimicrobial action. Hamamelis virginiana plant extract is known for its astringent, anti-inflammatory and antimicrobial properties. Thus, the present work aimed to incorporate aqueous (AE) and glycolic (GE) extracts of witch hazel in different concentrations to BC, aiming at its application as a curative. BC membranes incorporated with the extracts were characterized by thermogravimetric analysis (TGA), Fourier transforms infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and antimicrobial activity. The TGA results indicated a reduction in thermal stability and the appearance of a new stage of degradation in the samples that underwent incorporation. Furthermore, the FTIR showed the presence of aromatic compounds not found in the BC, corroborating the TGA. The micrographs revealed that the incorporation of the extracts resulted in the formation of a film on the surface of the membranes, covering the nanofibers. These results show that incorporating the aqueous and glycol extracts in the BC membrane was successful. However, despite the crude extracts’ antimicrobial potential, antimicrobial activity in the functionalized BC samples was not observed.
2.
TLR4 and TLR8 variability in Amazonian and West Indian manatee species from Brazil
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Oliveira, Tatiana Maia de
; Burlamaqui, Tibério Cesar Tortola
; Sá, André Luiz Alves de
; Breaux, Breanna
; Luna, Fábia de Oliveira
; Attademo, Fernanda Löffler Niemeyer
; Klautau, Alex Garcia Cavalleiro de Macedo
; Oliveira, Jairo Moura
; Sena, Leonardo
; Criscitiello, Michael F.
; Schneider, Maria Paula Cruz
.
Abstract Amazonian (Trichechus inunguis) and West Indian (Trichechus manatus) manatees are aquatic mammals vulnerable to extinction found in the Amazon basin and the coastal western Atlantic. Toll-like receptors (TLR) play a key role in recognizing pathogen-associated molecular patterns using leucine-rich repeats (LRRs). We described the diversity of TLR4 and TLR8 genes in these two species of manatee. Amazonian manatee showed seven SNPs in TLR4 and the eight in TLR8, while West Indian manatee shared four and six of those SNPs, respectively. In our analysis, TLR4 showed one non-conservative amino acid replacement substitution in LRR7 and LRR8, on the other hand, TLR8 was less variable and showed only conserved amino acid substitutions. Selection analysis showed that only one TLR4 site was subjected to positive selection and none in TLR8. TLR4 in manatees did not show any evidence of convergent evolution compared to species of the cetacean lineage. Differences in TLR4 and TLR8 polymorphism may be related to distinct selection by pathogens, population reduction of West Indian manatees, or an expected consequence of population expansion in Amazonian manatees. Future studies combining pathogen association and TLR polymorphism may clarify possible roles of these genes and be used for conservation purposes of manatee species.
https://doi.org/10.1590/1678-4685-gmb-2019-0252
448 downloads
3.
Stress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62A
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Fraiha, Rafael Ovando
; Pereira, Ana Paula Ramos
; Brito, Eliana da Costa Alvarenga
; Borges, Clayton Luiz
; Parente, Ana Flávia Alves
; Perdomo, Renata Trentin
; Macedo, Maria Ligia Rodrigues
; Weber, Simone Schneider
.
Revista da Sociedade Brasileira de Medicina Tropical
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Abstract INTRODUCTION: Studies have demonstrated that pathogens react to the harsh conditions in human tissues by inducing mechanisms that promote survival. METHODS: Persistence and biofilm-forming ability were evaluated during stress conditions that mimic those in the host. RESULTS: Carbon-source availability had a positive effect on Staphylococcus epidermidis RP62A adhesion during hypoxia, accompanied by a decrease in pH. In contrast, iron limitation led to decreased surface-adherent biomass, accompanied by an increase medium acidification and lactate levels. Interestingly, iron starvation and hypoxia induced persister cells in planktonic culture. CONCLUSIONS: These findings highlight the role of host stress in the virulence of S. epidermidis.
https://doi.org/10.1590/0037-8682-0001-2018
1610 downloads
4.
Comparative mitogenomic analyses of Amazona parrots and Psittaciformes
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Lima, Nicholas Costa Barroso
; Soares, André Elias Rodrigues
; Almeida, Luiz Gonzaga de Paula
; Costa, Igor Rodrigues da
; Sato, Fernanda Midori
; Schneider, Patricia
; Aleixo, Alexandre
; Schneider, Maria Paula
; Santos, Fabrício R.
; Mello, Claudio V.
; Miyaki, Cristina
; Vasconcelos, Ana Tereza R.
; Prosdocimi, Francisco
.
Abstract Amazon parrots are long-lived birds with highly developed cognitive skills, including vocal learning. Several parrot mitogenomes have been sequenced, but important aspects of their organization and evolution are not fully understood or have limited experimental support. The main aim of the present study was to describe the mitogenome of the blue-fronted Amazon, Amazona aestiva, and compare it to other mitogenomes from the genus Amazona and the order Psittaciformes. We observed that mitogenomes are highly conserved among Amazon parrots, and a detailed analysis of their duplicated control regions revealed conserved blocks. Population level analyses indicated that the specimen analyzed here seems to be close to A. aestiva individuals from Bahia state. Evolutionary relationships of 41 Psittaciformes species and three outgroups were inferred by BEAST. All relationships were retrieved with high support.
https://doi.org/10.1590/1678-4685-gmb-2017-0023
1973 downloads
5.
Impacto da sessão de hemodiálise na força de preensão manual
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Pinto, Ana Paula
; Ramos, Christiane Ishikawa
; Meireles, Marion Schneider
; Kamimura, Maria Ayako
; Cuppari, Lilian
.
Resumo Introdução: A força de preensão manual (FPM) é um método simples, confiável e com bom valor preditivo para avaliar a função muscular de pacientes submetidos à hemodiálise (HD). Porém, ainda não existe um consenso a respeito do momento mais apropriado para a aferição da medida, já que o desempenho da FPM pode ser influenciado pelas flutuações hidroeletrolíticas e de pressão arterial que acometem esses pacientes. Objetivo: Investigar o impacto da sessão de diálise sobre a FPM em pacientes submetidos à HD. Métodos: Trata-se de um estudo transversal com 156 pacientes [57,7% homens, idade mediana de 56,5 (42-67) anos, 28,8% diabéticos, IMC médio de 24,75 ± 4,5 kg/m2 e tempo em HD de 38 (19,25-72,75) meses]. Foram realizadas aferições da FPM com um dinamômetro nos minutos iniciais e ao término da sessão de HD. Os valores obtidos foram comparados com um padrão de referência nacional. Dados clínicos, demográficos e laboratoriais foram coletados do prontuário médico. Resultados: Foi observada uma redução significante da FPM após a sessão de HD (28,6 ± 11,4 kg para 27,7 ± 11,7 kg; p < 0,01). A prevalência de pacientes com FPM abaixo do percentil 30 aumentou de 44,9% para 55,1% (p < 0,01). A redução da pressão arterial durante a diálise foi o único fator que se associou com a redução da FPM. Conclusão: Os achados mostram que o processo de HD influencia negativamente a FPM.
Abstract Introduction: Handgrip strength (HGS) is a simple and reliable method with a good predictive clinical value for assessing muscle function of patients undergoing hemodialysis (HD). However, there is no consensus regarding the appropriate moment for performing the HGS measurement since the performance of the HGS can be influenced by fluid, electrolyte and blood pressure changes that affect patients on HD. Objective: To investigate the impact of the dialysis session on the HGS in patients undergoing HD. Methods: This is a cross-sectional study with 156 patients [57.7% male, median age of 56.5 (42-67) years old, 28.8% diabetes, mean BMI of 24.75 ± 4.5 kg/m2 and HD vintage of 38 (19.25 to 72.75) months]. Measures of HGS were performed with a dynamometer during the initial minutes of the HD session and at the end of the session. The values obtained were compared with a national standard reference. Clinical, demographic and laboratory data were collected from medical records. Results: A significant reduction of HGS was observed after the HD session (28.6 ± 11.4 kg to 27.7 ± 11.7 kg; p < 0.01). The prevalence of patients with HGS below the 30th percentile increased from 44.9% to 55.1% (p < 0.01). The decrease in blood pressure during dialysis was the only factor associated with the reduction of HGS. Conclusion: These findings show that the HD procedure affects negatively the HGS.
https://doi.org/10.5935/0101-2800.20150072
5002 downloads
6.
A putative RA-like region in the brain of the scale-backed antbird, Willisornis poecilinotus (Furnariides, Suboscines, Passeriformes, Thamnophilidae)
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Lima, Jamily L.R. de
; Soares, Fabricio A.
; Remedios, Ana C.S.
; Thom, Gregory
; Wirthlin, Morgan
; Aleixo, Alexandre
; Schneider, Maria Paula C.
; Mello, Claudio V.
; Schneider, Patricia N.
.
The memorization and production of song in songbirds share important parallels with the process of speech acquisition in humans. In songbirds, these processes are dependent on a group of specialized telencephalic nuclei known as the song system: HVC (used as a proper name), RA (robust nucleus of arcopallium), LMAN (lateral magnocellular nucleus of the nidopallium) and striatal Area X. A recent study suggested that the arcopallium of the Sayornis phoebe, a non vocal learner suboscine species, contains a nucleus with some properties similar to those of songbird RA, suggesting that the song system may have been present in the last common ancestor of these groups. Here we report morphological and gene expression evidence that a region with some properties similar to RA is present in another suboscine, the Amazonian endemic Willisornis poecilinotus. Specifically, a discrete domain with a distinct Nissl staining pattern and that expresses the RA marker RGS4 was found in the arcopallium where the oscine RA is localized. Our findings, combined with the previous report on the S. phoebe, suggest that an arcopallial region with some RA-like properties was present in the ancestor of both Suboscines infraorders Tyranni and Furnarii, and is possibly an ancestral feature of Passeriformes.
https://doi.org/10.1590/S1415-475738320150010
1790 downloads
7.
Estabilidade lipídica de filés de carpa húngara congelados tratados com extratos de Lippia Alba
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Veeck, Ana Paula de Lima
; Klein, Bruna
; Ruviaro, Amanda Roggia
; Quatrin, Andréia
; Ferreira, Lauren Fresinghelli
; Daniel, Ana Paula
; Piccolo, Jaqueline
; Oliveira, Maurício Schneider
; Mallmann, Carlos Augusto
; Heinzmann, Berta Maria
; Emanuelli, Tatiana
.
O objetivo do presente estudo foi avaliar o efeito da aspersão de extratos de Lippia alba na estabilidade lipídica de filés de carpa húngara armazenados a -18±2°C. Filés não tratados (controle sem aspersão) ou aspergidos (1mL 10g-1 de filé) com água destilada (controle água destilada) ou com extratos de L. alba (0,10g mL-1) hidrometanólico ou aquoso foram analisados durante o armazenamento nos dias zero, 90 e 180. Independente do tempo de congelamento, o extrato hidrometanólico reduziu os valores de dienos conjugados (DC) dos filés em relação aos demais tratamentos, além de reduzir os valores de ácidos graxos livres aos 90 dias (P<0,05). O extrato aquoso resultou em maior teor de peróxidos após 180 dias de congelamento comparado aos demais tratamentos (P<0,05). Os extratos hidrometanólico e aquoso reduziram os valores de substâncias reativas ao ácido tiobarbitúrico (TBARS) após 180 dias, comparados aos filés tratados com água destilada e sem aspersão (P<0,05). Ambos os extratos de L. alba retardaram a oxidação lipídica, sendo que o extrato aquoso retardou a degradação de produtos primários da oxidação lipídica (peróxidos) em produtos secundários (TBARS), enquanto o extrato hidrometanólico parece ser mais eficiente, pois inibiu de forma similar tanto a formação de DC e peróxidos, quanto a sua degradação em produtos secundários (TBARS)
The effect of sprinkling with Lippia alba extracts was evaluated on the lipid stability of common carp fillets stored at -18±2°C. Fillets that received no treatment (no spray control) or that were sprayed (1mL 10g-1 fillet) with distilled water (water control) or with hydro-methanolic or aqueous extract of L. alba (0.10g mL-1) were evaluated immediately (time zero) and after 90 and 180 days. Regardless of the storage time, the hydro-methanolic extract reduced the conjugated dienes (CD) values of fillets compared to the other treatments, and reduced the free fatty acid levels at 90 days (P<0.05). The aqueous extract caused higher peroxide value after 180 days of frozen storage compared to the other treatments (P<0.05). The hydro-methanolic and aqueous extracts reduced thiobarbituric acid reactive substances (TBARS) values after 180 days compared to the non-treated fillets or to the water-sprayed fillets (P<0.05). Both extracts have delayed lipid oxidation. While the aqueous L. alba extract delayed the degradation of primary oxidation products (peroxides) into secondary products (TBARS), the hydro-methanolic extract was more efficient as it inhibited both the CD and peroxide formation and its degradation into secondary products (TBARS)
https://doi.org/10.1590/0103-8478cr20140785
1598 downloads
8.
Growing knowledge: an overview of Seed Plant diversity in Brazil
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Zappi, Daniela C.
; Filardi, Fabiana L. Ranzato
; Leitman, Paula
; Souza, Vinícius C.
; Walter, Bruno M.T.
; Pirani, José R.
; Morim, Marli P.
; Queiroz, Luciano P.
; Cavalcanti, Taciana B.
; Mansano, Vidal F.
; Forzza, Rafaela C.
; Abreu, Maria C.
; Acevedo-Rodríguez, Pedro
; Agra, Maria F.
; Almeida Jr., Eduardo B.
; Almeida, Gracineide S.S.
; Almeida, Rafael F.
; Alves, Flávio M.
; Alves, Marccus
; Alves-Araujo, Anderson
; Amaral, Maria C.E.
; Amorim, André M.
; Amorim, Bruno
; Andrade, Ivanilza M.
; Andreata, Regina H.P.
; Andrino, Caroline O.
; Anunciação, Elisete A.
; Aona, Lidyanne Y.S.
; Aranguren, Yani
; Aranha Filho, João L.M.
; Araújo, Andrea O.
; Araújo, Ariclenes A.M.
; Araújo, Diogo
; Arbo, María M.
; Assis, Leandro
; Assis, Marta C.
; Assunção, Vivian A.
; Athiê-Souza, Sarah M.
; Azevedo, Cecilia O.
; Baitello, João B.
; Barberena, Felipe F.V.A.
; Barbosa, Maria R.V.
; Barros, Fábio
; Barros, Lucas A.V.
; Barros, Michel J.F.
; Baumgratz, José F.A.
; Bernacci, Luis C.
; Berry, Paul E.
; Bigio, Narcísio C.
; Biral, Leonardo
; Bittrich, Volker
; Borges, Rafael A.X.
; Bortoluzzi, Roseli L.C.
; Bove, Cláudia P.
; Bovini, Massimo G.
; Braga, João M.A.
; Braz, Denise M.
; Bringel Jr., João B.A.
; Bruniera, Carla P.
; Buturi, Camila V.
; Cabral, Elza
; Cabral, Fernanda N.
; Caddah, Mayara K.
; Caires, Claudenir S.
; Calazans, Luana S.B.
; Calió, Maria F.
; Camargo, Rodrigo A.
; Campbell, Lisa
; Canto-Dorow, Thais S.
; Carauta, Jorge P.P.
; Cardiel, José M.
; Cardoso, Domingos B.O.S.
; Cardoso, Leandro J.T.
; Carneiro, Camila R.
; Carneiro, Cláudia E.
; Carneiro-Torres, Daniela S.
; Carrijo, Tatiana T.
; Caruzo, Maria B.R.
; Carvalho, Maria L.S.
; Carvalho-Silva, Micheline
; Castello, Ana C.D.
; Cavalheiro, Larissa
; Cervi, Armando C.
; Chacon, Roberta G.
; Chautems, Alain
; Chiavegatto, Berenice
; Chukr, Nádia S.
; Coelho, Alexa A.O.P.
; Coelho, Marcus A.N.
; Coelho, Rubens L.G.
; Cordeiro, Inês
; Cordula, Elizabeth
; Cornejo, Xavier
; Côrtes, Ana L.A.
; Costa, Andrea F.
; Costa, Fabiane N.
; Costa, Jorge A.S.
; Costa, Leila C.
; Costa-e-Silva, Maria B.
; Costa-Lima, James L.
; Cota, Maria R.C.
; Couto, Ricardo S.
; Daly, Douglas C.
; De Stefano, Rodrigo D.
; De Toni, Karen
; Dematteis, Massimiliano
; Dettke, Greta A.
; Di Maio, Fernando R.
; Dórea, Marcos C.
; Duarte, Marília C.
; Dutilh, Julie H.A.
; Dutra, Valquíria F.
; Echternacht, Lívia
; Eggers, Lilian
; Esteves, Gerleni
; Ezcurra, Cecilia
; Falcão Junior, Marcus J.A.
; Feres, Fabíola
; Fernandes, José M.
; Ferreira, D.M.C.
; Ferreira, Fabrício M.
; Ferreira, Gabriel E.
; Ferreira, Priscila P.A.
; Ferreira, Silvana C.
; Ferrucci, Maria S.
; Fiaschi, Pedro
; Filgueiras, Tarciso S.
; Firens, Marcela
; Flores, Andreia S.
; Forero, Enrique
; Forster, Wellington
; Fortuna-Perez, Ana P.
; Fortunato, Reneé H.
; Fraga, Cléudio N.
; França, Flávio
; Francener, Augusto
; Freitas, Joelcio
; Freitas, Maria F.
; Fritsch, Peter W.
; Furtado, Samyra G.
; Gaglioti, André L.
; Garcia, Flávia C.P.
; Germano Filho, Pedro
; Giacomin, Leandro
; Gil, André S.B.
; Giulietti, Ana M.
; A.P.Godoy, Silvana
; Goldenberg, Renato
; Gomes da Costa, Géssica A.
; Gomes, Mário
; Gomes-Klein, Vera L.
; Gonçalves, Eduardo Gomes
; Graham, Shirley
; Groppo, Milton
; Guedes, Juliana S.
; Guimarães, Leonardo R.S.
; Guimarães, Paulo J.F.
; Guimarães, Elsie F.
; Gutierrez, Raul
; Harley, Raymond
; Hassemer, Gustavo
; Hattori, Eric K.O.
; Hefler, Sonia M.
; Heiden, Gustavo
; Henderson, Andrew
; Hensold, Nancy
; Hiepko, Paul
; Holanda, Ana S.S.
; Iganci, João R.V.
; Imig, Daniela C.
; Indriunas, Alexandre
; Jacques, Eliane L.
; Jardim, Jomar G.
; Kamer, Hiltje M.
; Kameyama, Cíntia
; Kinoshita, Luiza S.
; Kirizawa, Mizué
; Klitgaard, Bente B.
; Koch, Ingrid
; Koschnitzke, Cristiana
; Krauss, Nathália P.
; Kriebel, Ricardo
; Kuntz, Juliana
; Larocca, João
; Leal, Eduardo S.
; Lewis, Gwilym P.
; Lima, Carla T.
; Lima, Haroldo C.
; Lima, Itamar B.
; Lima, Laíce F.G.
; Lima, Laura C.P.
; Lima, Leticia R.
; Lima, Luís F.P.
; Lima, Rita B.
; Lírio, Elton J.
; Liro, Renata M.
; Lleras, Eduardo
; Lobão, Adriana
; Loeuille, Benoit
; Lohmann, Lúcia G.
; Loiola, Maria I.B.
; Lombardi, Julio A.
; Longhi-Wagner, Hilda M.
; Lopes, Rosana C.
; Lorencini, Tiago S.
; Louzada, Rafael B.
; Lovo, Juliana
; Lozano, Eduardo D.
; Lucas, Eve
; Ludtke, Raquel
; Luz, Christian L.
; Maas, Paul
; Machado, Anderson F.P.
; Macias, Leila
; Maciel, Jefferson R.
; Magenta, Mara A.G.
; Mamede, Maria C.H.
; Manoel, Evelin A.
; Marchioretto, Maria S.
; Marques, Juliana S.
; Marquete, Nilda
; Marquete, Ronaldo
; Martinelli, Gustavo
; Martins da Silva, Regina C.V.
; Martins, Ângela B.
; Martins, Erika R.
; Martins, Márcio L.L.
; Martins, Milena V.
; Martins, Renata C.
; Matias, Ligia Q.
; Maya-L., Carlos A.
; Mayo, Simon
; Mazine, Fiorella
; Medeiros, Debora
; Medeiros, Erika S.
; Medeiros, Herison
; Medeiros, João D.
; Meireles, José E.
; Mello-Silva, Renato
; Melo, Aline
; Melo, André L.
; Melo, Efigênia
; Melo, José I.M.
; Menezes, Cristine G.
; Menini Neto, Luiz
; Mentz, Lilian A.
; Mezzonato, A.C.
; Michelangeli, Fabián A.
; Milward-de-Azevedo, Michaele A.
; Miotto, Silvia T.S.
; Miranda, Vitor F.O.
; Mondin, Cláudio A.
; Monge, Marcelo
; Monteiro, Daniele
; Monteiro, Raquel F.
; Moraes, Marta D.
; Moraes, Pedro L.R.
; Mori, Scott A.
; Mota, Aline C.
; Mota, Nara F.O.
; Moura, Tania M.
; Mulgura, Maria
; Nakajima, Jimi N.
; Nardy, Camila
; Nascimento Júnior, José E.
; Noblick, Larry
; Nunes, Teonildes S.
; O'Leary, Nataly
; Oliveira, Arline S.
; Oliveira, Caetano T.
; Oliveira, Juliana A.
; Oliveira, Luciana S.D.
; Oliveira, Maria L.A.A.
; Oliveira, Regina C.
; Oliveira, Renata S.
; Oliveira, Reyjane P.
; Paixão-Souza, Bruno
; Parra, Lara R.
; Pasini, Eduardo
; Pastore, José F.B.
; Pastore, Mayara
; Paula-Souza, Juliana
; Pederneiras, Leandro C.
; Peixoto, Ariane L.
; Pelissari, Gisela
; Pellegrini, Marco O.O.
; Pennington, Toby
; Perdiz, Ricardo O.
; Pereira, Anna C.M.
; Pereira, Maria S.
; Pereira, Rodrigo A.S.
; Pessoa, Clenia
; Pessoa, Edlley M.
; Pessoa, Maria C.R.
; Pinto, Luiz J.S.
; Pinto, Rafael B.
; Pontes, Tiago A.
; Prance, Ghillean T.
; Proença, Carolyn
; Profice, Sheila R.
; Pscheidt, Allan C.
; Queiroz, George A.
; Queiroz, Rubens T.
; Quinet, Alexandre
; Rainer, Heimo
; Ramos, Eliana
; Rando, Juliana G.
; Rapini, Alessandro
; Reginato, Marcelo
; Reis, Ilka P.
; Reis, Priscila A.
; Ribeiro, André R.O.
; Ribeiro, José E.L.S.
; Riina, Ricarda
; Ritter, Mara R.
; Rivadavia, Fernando
; Rocha, Antônio E.S.
; Rocha, Maria J.R.
; Rodrigues, Izabella M.C.
; Rodrigues, Karina F.
; Rodrigues, Rodrigo S.
; Rodrigues, Rodrigo S.
; Rodrigues, Vinícius T.
; Rodrigues, William
; Romaniuc Neto, Sérgio
; Romão, Gerson O.
; Romero, Rosana
; Roque, Nádia
; Rosa, Patrícia
; Rossi, Lúcia
; Sá, Cyl F.C.
; Saavedra, Mariana M.
; Saka, Mariana
; Sakuragui, Cássia M.
; Salas, Roberto M.
; Sales, Margareth F.
; Salimena, Fatima R.G.
; Sampaio, Daniela
; Sancho, Gisela
; Sano, Paulo T.
; Santos, Alessandra
; Santos, Élide P.
; Santos, Juliana S.
; Santos, Marianna R.
; Santos-Gonçalves, Ana P.
; Santos-Silva, Fernanda
; São-Mateus, Wallace
; Saraiva, Deisy P.
; Saridakis, Dennis P.
; Sartori, Ângela L.B.
; Scalon, Viviane R.
; Schneider, Ângelo
; Sebastiani, Renata
; Secco, Ricardo S.
; Senna, Luisa
; Senna-Valle, Luci
; Shirasuna, Regina T.
; Silva Filho, Pedro J.S.
; Silva, Anádria S.
; Silva, Christian
; Silva, Genilson A.R.
; Silva, Gisele O.
; Silva, Márcia C.R.
; Silva, Marcos J.
; Silva, Marcos J.
; Silva, Otávio L.M.
; Silva, Rafaela A.P.
; Silva, Saura R.
; Silva, Tania R.S.
; Silva-Gonçalves, Kelly C.
; Silva-Luz, Cíntia L.
; Simão-Bianchini, Rosângela
; Simões, André O.
; Simpson, Beryl
; Siniscalchi, Carolina M.
; Siqueira Filho, José A.
; Siqueira, Carlos E.
; Siqueira, Josafá C.
; Smith, Nathan P.
; Snak, Cristiane
; Soares Neto, Raimundo L.
; Soares, Kelen P.
; Soares, Marcos V.B.
; Soares, Maria L.
; Soares, Polyana N.
; Sobral, Marcos
; Sodré, Rodolfo C.
; Somner, Genise V.
; Sothers, Cynthia A.
; Sousa, Danilo J.L.
; Souza, Elnatan B.
; Souza, Élvia R.
; Souza, Marcelo
; Souza, Maria L.D.R.
; Souza-Buturi, Fátima O.
; Spina, Andréa P.
; Stapf, María N.S.
; Stefano, Marina V.
; Stehmann, João R.
; Steinmann, Victor
; Takeuchi, Cátia
; Taylor, Charlotte M.
; Taylor, Nigel P.
; Teles, Aristônio M.
; Temponi, Lívia G.
; Terra-Araujo, Mário H.
; Thode, Veronica
; Thomas, W.Wayt
; Tissot-Squalli, Mara L.
; Torke, Benjamin M.
; Torres, Roseli B.
; Tozzi, Ana M.G.A.
; Trad, Rafaela J.
; Trevisan, Rafael
; Trovó, Marcelo
; Valls, José F.M.
; Vaz, Angela M.S.F.
; Versieux, Leonardo
; Viana, Pedro L.
; Vianna Filho, Marcelo D.M.
; Vieira, Ana O.S.
; Vieira, Diego D.
; Vignoli-Silva, Márcia
; Vilar, Thaisa
; Vinhos, Franklin
; Wallnöfer, Bruno
; Wanderley, Maria G.L.
; Wasshausen, Dieter
; Watanabe, Maurício T.C.
; Weigend, Maximilian
; Welker, Cassiano A.D.
; Woodgyer, Elizabeth
; Xifreda, Cecilia C.
; Yamamoto, Kikyo
; Zanin, Ana
; Zenni, Rafael D.
; Zickel, Carmem S
.
Resumo Um levantamento atualizado das plantas com sementes e análises relevantes acerca desta biodiversidade são apresentados. Este trabalho se iniciou em 2010 com a publicação do Catálogo de Plantas e Fungos e, desde então vem sendo atualizado por mais de 430 especialistas trabalhando online. O Brasil abriga atualmente 32.086 espécies nativas de Angiospermas e 23 espécies nativas de Gimnospermas e estes novos dados mostram um aumento de 3% da riqueza em relação a 2010. A Amazônia é o Domínio Fitogeográfico com o maior número de espécies de Gimnospermas, enquanto que a Floresta Atlântica possui a maior riqueza de Angiospermas. Houve um crescimento considerável no número de espécies e nas taxas de endemismo para a maioria dos Domínios (Caatinga, Cerrado, Floresta Atlântica, Pampa e Pantanal), com exceção da Amazônia que apresentou uma diminuição de 2,5% de endemicidade. Entretanto, a maior parte das plantas com sementes que ocorrem no Brasil (57,4%) é endêmica deste território. A proporção de formas de vida varia de acordo com os diferentes Domínios: árvores são mais expressivas na Amazônia e Floresta Atlântica do que nos outros biomas, ervas são dominantes no Pampa e as lianas apresentam riqueza expressiva na Amazônia, Floresta Atlântica e Pantanal. Este trabalho não só quantifica a biodiversidade brasileira, mas também indica as lacunas de conhecimento e o desafio a ser enfrentado para a conservação desta flora.
Abstract An updated inventory of Brazilian seed plants is presented and offers important insights into the country's biodiversity. This work started in 2010, with the publication of the Plants and Fungi Catalogue, and has been updated since by more than 430 specialists working online. Brazil is home to 32,086 native Angiosperms and 23 native Gymnosperms, showing an increase of 3% in its species richness in relation to 2010. The Amazon Rainforest is the richest Brazilian biome for Gymnosperms, while the Atlantic Rainforest is the richest one for Angiosperms. There was a considerable increment in the number of species and endemism rates for biomes, except for the Amazon that showed a decrease of 2.5% of recorded endemics. However, well over half of Brazillian seed plant species (57.4%) is endemic to this territory. The proportion of life-forms varies among different biomes: trees are more expressive in the Amazon and Atlantic Rainforest biomes while herbs predominate in the Pampa, and lianas are more expressive in the Amazon, Atlantic Rainforest, and Pantanal. This compilation serves not only to quantify Brazilian biodiversity, but also to highlight areas where there information is lacking and to provide a framework for the challenge faced in conserving Brazil's unique and diverse flora.
https://doi.org/10.1590/2175-7860201566411
33340 downloads
9.
Comparative study of anthropometric and body composition variables, and functionality between elderly that perform regular or irregular physical activity
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Leite, Leni Everson Araújo
; Cruz, Ivana Beatrice Mânica da
; Baptista, Rafael
; Heidner, Gustavo Sandri
; Rosemberg, Laura
; Nogueira, Guilherme
; Closs, Vera Elizabeth
; Engroff, Paula
; Viegas, Karin
; Schneider, Rodolfo
; Gottlieb, Maria Gabriela Valle
.
Revista Brasileira de Geriatria e Gerontologia
- Journal Metrics
Introdução: o impacto da atividade física regular e/ou irregular na composição corporal, massa e força muscular e funcionalidade em idosos é ainda pouco estudado. Objetivo: comparar variáveis antropométricas, espessura muscular e de gordura, mobilidade e força de preensão palmar de membros inferiores entre idosos regular e irregularmente ativos classificados pelo International Phisical Activity Questionnnaire. Método: estudo transversal com 75 idosos (14 homens e 61 mulheres) que praticavam atividade física regular (AFR=10) ou irregular (AFI=65). Variáveis antropométricas (índice de massa corporal, circunferências e dobras cutâneas), espessura muscular e de gordura (tríceps braquial, vasto lateral e gastrocnêmico medial [ultrassom]), força de preensão palmar (dinamômetro Crown), força de membros inferiores (teste de senta e levanta) e mobilidade foram coletadas da amostra de idosos. A atividade física foi avaliada através do International Phisical Activity Questionnnaire. Resultados: não foram encontradas diferenças significativas nas variáveis antropométricas, espessura muscular e de gordura e força entre os dois grupos (p>0,05). No entanto, no grupo de idosos que praticavam atividade física irregular, foi obtido melhor desempenho no teste de mobilidade do que nos idosos que praticavam atividade física regular (p<0,008). Esse resultado foi independente de sexo e idade (p=0,017). Conclusão: o estudo não encontrou diferenças significativas entre os idosos que praticam atividade física de forma regular ou irregular em relação à composição corporal e parâmetros de força. Contudo, os resultados sugerem que a prática de atividade física irregular pode ajudar a mobilidade dos idosos e prevenir quedas.
Background: The impact of regular and irregular physical activity in body composition, muscle mass and strength of the elderly is not well studied yet. Objective: To compare anthropometric variables, muscle and fat thickness, mobility, handgrip and lower limb strength between regularly and irregularly active elderly classified by the International Physical Questionnaire Activity Questionnaire. Methods: A cross-sectional study conducted with 75 elderly people (14 males and 61 females) who practiced regular (RPA=10) or irregular physical activity (IPA=65). Anthropometric variables (body mass index, circumferences and skinfolds), muscular and fat thickness (triceps, vastus lateralis and medial gastrocnemius [ultrasound]), handgrip strength (Crown dynamometer), lower limb strength (sit and stand up test) and mobility were collected from the sample. Physical activity was assessed by the International Physical Questionnaire Activity Questionnaire. Results: No significant differences were found in the anthropometric, skeletal muscle and fat mass and force variables between two groups (p>0.05). However, elders who practiced irregular physical activity presented best performance in the time up and go test than those who practiced regular physical activity (p=0.008). Results were independent of sex and age of subjects (p=0.017). Conclusion: The study showed no significant differences between elderly that performed physical activity in regular or irregular way in relation body composition and force parameters. However, the results suggest that even irregular physical activity can help the elderly individuals in the mobility, and prevent falls.
https://doi.org/10.1590/S1809-98232014000100004
5561 downloads
10.
Diversity and three-dimensional structures of the alpha Mcr of the methanogenic Archaea from the anoxic region of Tucuruí Lake, in Eastern Brazilian Amazonia
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Santana, Priscila Bessa
; Ghilardi Junior, Rubens
; Alves, Claudio Nahum
; Silva, Jeronimo Lameira
; McCulloch, John Anthony
; Schneider, Maria Paula Cruz
; Silva, Artur da Costa da
.
Methanogenic archaeans are organisms of considerable ecological and biotechnological interest that produce methane through a restricted metabolic pathway, which culminates in the reaction catalyzed by the Methyl-coenzyme M reductase (Mcr) enzyme, and results in the release of methane. Using a metagenomic approach, the gene of the a subunit of mcr (mcrα) was isolated from sediment sample from an anoxic zone, rich in decomposing organic material, obtained from the Tucuruí hydroelectric dam reservoir in eastern Brazilian Amazonia. The partial nucleotide sequences obtained were 83 to 95% similar to those available in databases, indicating a low diversity of archaeans in the reservoir. Two orders were identified -the Methanomicrobiales, and a unique Operational Taxonomic Unit (OTU) forming a clade with the Methanosarcinales according to low bootstrap values. Homology modeling was used to determine the three-dimensional (3D) structures, for this the partial nucleotide sequence of the mcrα were isolated and translated on their partial amino acid sequences. The 3D structures of the archaean mcrα observed in the present study varied little, and presented approximately 70% identity in comparison with the mcrα of Methanopyrus klanderi. The results demonstrated that the community of methanogenic archaeans of the anoxic C1 region of the Tucurui reservoir is relatively homogeneous.
2669 downloads
11.
Genetic structure of red-handed howler monkey populations in the fragmented landscape of Eastern Brazilian Amazonia
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Bastos, Heitor B.
; Gonçalves, Evonnildo C.
; Ferrari, Stephen F.
; Silva, Artur
; Schneider, Maria Paula C.
.
We genotyped 15 microsatellite loci in order to evaluate the effects of habitat fragmentation, caused by flooding of the Tucuruí reservoir, on the genetic structure of Alouatta belzebul in eastern Amazonia. The analysis included two populations sampled in 1984, representing both margins of the Tocantins river, and three populations sampled 18 years later. Minimal differences in the diversity levels between present-day (Ho = 0.62-0.69 and A R = 6.07-7.21) and pre-flooding (Ho = 0.60-0.62 and A R = 6.27-6.77) populations indicated there was no significant loss of genetic variability, possibly because of successful management strategies applied during the flooding. The changes observed were limited to shifts in the composition of alleles, which presumably reflect the admixture of subpopulations during flooding. Given this, there were significant differences in the Rst values (p = 0.05) in all but one between-site comparison. Both present-day and original populations showed a deficit of heterozygotes, which suggests that this may be typical of the species, at least at a local level, perhaps because of specific ecological characteristics. The relatively large number of private alleles recorded in all populations may be a consequence of the Wahlund effect resulting from population admixture or a process of expansion rather than the loss of rare alleles through genetic drift. Additionally, the levels of genetic variability observed in this study were higher than those reported for other species of Neotropical primates, suggesting good fitness levels in these A. belzebul populations. Regular genetic monitoring of remnant populations, especially on islands, should nevertheless be an integral component of long-term management strategies.
2561 downloads
12.
Executive functions in the young elderly and oldest old: a preliminary comparison emphasizing decision making
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Bakos, Daniela Schneider
; Couto, Maria Clara Pinheiro de Paula
; Melo, Wilson Vieira
; Parente, Maria Alice de M. P.
; Koller, Silvia H.
; Bizarro, Lisiane
.
This study aimed at investigating differences in the performance of the young elderly and oldest old in tasks evaluating cognitive flexibility/inhibition (Stroop test), selective attention/working memory (Digit Span Subtest), premorbid intelligence/semantic knowledge (Vocabulary Subtest), and decision making (Iowa Gambling Task - IGT). Twenty healthy individuals were divided into two groups: 10 young elderly (M = 62 years, SD = 2.1) and 10 oldest old (M = 80 years, SD = 3.3), both with high educational level (M = 14 years of study, SD = 2.5). Results showed that the groups were only different in terms of decision making. There was also a difference in the learning process of each group; the young elderly reached more favorable scores in the IGT.
2636 downloads
13.
Meiose e viabilidade polínica na família Araceae
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Corrêa, Maria Goreti Senna
; Viégas, Judith
; Silva, João Baptista da
; Ávila, Paula Fernanda Vaz de
; Busato, Gustavo Rossato
; Lemes, Jaqueline Schneider
.
O objetivo deste trabalho foi analisar a microsporogênese e a viabilidade dos grãos de pólen em 17 espécies de aráceas coletadas no Rio Grande do Sul, Brasil. Nove espécies foram analisadas quanto à ocorrência de células mãe de pólen (CMP) normais e anormais nas fases de metáfase, anáfase e telófase, tanto da meiose I (M I) como da meiose II (M II); 10 espécies foram estudadas quanto à presença de tétrades com número normal ou anormal de micrósporos e 17 espécies quanto à viabilidade dos grãos de pólen. As CMP anormais apresentaram, tanto em M I quanto em M II, cromossomos fora da placa metafásica ou cromossomos retardatários em anáfase e/ou telófase. As freqüências de CMP normais/anormais encontradas na microsporogênese salientam a grande variação existente entre as espécies. Ressalta-se a ausência de CMP com anomalias na microsporogênese de Monstera deliciosa Adans., assim como em M I de Anthurium scandens (Aubl) Engl. e em M II de Caladium hortulanum Birdsey. O número observado de CMP anômalas, em M I e M II, nas espécies Syngonium podophyllum Schott e Zantedeschia aethiopica Spreng, foi maior que o esperado. A freqüência média de tétrades normais em dez espécies de aráceas, assim como a de grãos de pólen viáveis em 17 espécies, foi significativamente superior à freqüência média de anormais e de inviáveis, respectivamente.
The objective of this work was to analyze microsporogenesis and pollen viability in 17 species of the Araceae family collected at Rio Grande do Sul, Brazil. Occurrence of normal and abnormal pollen mother cells (PMC) was analyzed in metaphase, anaphase and telophase, in meiosis I (M I) and meiosis II (M II) of nine species; tetrads with normal or abnormal number of microspores was observed in 10 species, and pollen grain viability, in 17 species. Abnormal PMC presented chromosomes outside the metaphasic plate or laggard chromosomes in anaphase and/or telophase in both M I and M II. The frequencies of normal/abnormal PMC found in microsporogenesis point out to the existence of great variation among the species. It should be emphasized the absence of PMC with abnormalities in Monstera deliciosa Adans. microsporogenesis, as well as in Anthurium scandens (Aubl) Engl. M I and in Caladium hortulanum Birdsey M II. The number of anomalous PMC in Syngonium podophyllum Schott and Zantedeschia aethiopica Spreng in M I and M IIwas higher than expected. Average frequency of normal tetrads in ten araceous species and viable pollen grains in 17 species were significantly superior to the average frequency of abnormal tetrads and unviable pollen grains, respectively.
14.
Molecular phylogeny of the genus Saguinus (Platyrrhini, Primates) based on the ND1 mitochondrial gene and implications for conservation
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Tagliaro, Claudia Helena
; Schneider, Horacio
; Sampaio, Iracilda
; Schneider, Maria Paula Cruz
; Vallinoto, Marcelo
; Stanhope, Michael
.
The systematics of the subfamily Callitrichinae (Platyrrhini, Primates), a group of small monkeys from South America and Panama, remains an area of considerable discussion despite many investigations, there being continuing controversy over subgeneric taxonomic classifications based on morphological characters. The purpose of our research was to help elucidate the phylogenetic relationships within the monkey genus Saguinus (Callitrichinae) using a molecular approach to discover whether or not the two different sections containing hairy-faced and bare-faced species are monophyletic, whether Saguinus midas midas and Saguinus bicolor are more closely related than are S. midas midas and Saguinus midas niger, and if Saguinus fuscicollis melanoleucus and Saguinus fuscicollis weddelli really are different species. We sequenced the 957 bp ND1 mitochondrial gene of 21 Saguinus monkeys (belonging to six species and nine morphotypes) and one Cebus monkey (the outgroup) and constructed phylogenetic trees using maximum parsimony, neighbor joining, and maximum likelihood methods. The phylogenetic trees obtained divided the genus Saguinus into two groups, one containing the small-bodied species S. fuscicollis and the other, the large-bodied species S. mystax, S. leucopus, S. oedipus, S. midas, S. bicolor. The most derived taxa, S. midas and S. bicolor, grouped together, while S. fuscicollis melanoleucus and S. f. weddelli showed divergence values that did not support the division of these morphotypes into subspecies. On the other hand, S. midas individuals showed divergence compatible with the existence of three subspecies, two of them with the same morphotype as the subspecies S. midas niger. The results of our study suggest that there is at least one Saguinus subspecies that has not yet been described and that the conservation status of Saguinus species and subspecies should be carefully revised using modern molecular approaches.
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15.
Taxonomic implications of molecular studies on Northern Brazilian Teredinidae (Mollusca: Bivalvia) specimens
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Santos, Sonia Maria Lima
; Tagliaro, Claudia Helena
; Beasley, Colin Robert
; Schneider, Horacio
; Sampaio, Iracilda
; Santos Filho, Carlos
; Müller, Ana Cláudia de Paula
.
The current taxonomy of the Teredinidae (shipworms) is wholly based on morphology and up to now no molecular studies of the phylogeny of this group have been published. In the present study the relationships between four genera of the subfamilies Teredininae and Bankiinae were established and the efficiency of the 16S rRNA gene in characterizing four Teredinidae species was tested. Phylogenetic trees support the grouping of Bankia fimbriatula with Nausitora fusticula and of Neoteredo reynei with Psiloteredo healdi, but the genetic distances do not justify the classification of these species into two distinct subfamilies. The results show that B. fimbriatula, N. reynei and P. healdi specimens from the coast of the Brazilian state of Pará have five distinct 16S rRNA haplotypes, with one N. reynei haplotype differing from the other haplotypes in respect to at least seven sequences sites, indicating the existence of two very distinct sympatric lineages.
2971 downloads
Cited 1 time in SciELO
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