Resumo Contexto: Tendo por base os estudos do Modelo Multifatorial Teresa Lluch (1999), foi criado e validado um Programa Promotor Saúde Mental Positiva (SM+) para adolescentes e aplicado numa turma piloto. Após 3 meses do final do programa, procedeu-se ao follow-up na mesma turma, dando origem a novos dados. O trabalho integra-se Projeto Saúde Mental Positiva: CINTESIS/NursID: Centro de Investigação em Tecnologias e Serviços de Saúde/ Escola Superior de Enfermagem do Porto. Objetivo(s): Avaliar o impacto do programa de SM+ em adolescentes após 3 meses do seu término, analisar a eficácia do programa SM+ na promoção da saúde mental dos adolescentes em ambiente escolar e avaliar a vulnerabilidade psicológica dos adolescentes. Métodos: Estudo quantitativo, observacional-descritivo e longitudinal. A amostra foi constituída por 23 alunos com idades entre os 14-16 anos, e como instrumento foi aplicado novamente Escala de Vulnerabilidade Psicológica (EVP) e o Questionário de Saúde Mental Positiva (QSM+). Resultados: Antes do programa o QSM+ da turma era de 94,35, após programa passou a 92,61. Após 3 meses, QSM+ passou para 91,17. Cotações baixas no QSM+ correspondem a níveis mais elevados de SMP, variando entre 39 e 156. Na EVP registou-se alterações, passando de 14,26 após o programa para 15,39 no follow-up. Conclusões: Apesar das diferenças observadas não terem significado estatístico, que nos permita afirmar que o Programa de SM+ teve eficácia na saúde mental dos adolescentes e na sua vulnerabilidade psicológica, foram observadas mudanças a nível comportamental, com maior interajuda entre eles, melhor comunicação e mais ativos nas atividades escolares.

Abstract Background: Based on studies of Teresa Lluch's Multifactorial Model (1999), a Positive Mental Health Promoting Program for adolescents was created and validated and applied to a pilot class. Three months after the end of the program, we followed up in the same class, giving rise to new data. The work is part of the Positive Mental Health Project of CINTESIS / NursID of the Research Center on Health Technologies and Services and the Porto School of Nursing. Aim: To assess the impact of the positive mental health (PMH) program on adolescents 3 months after its end; analyze the effectiveness of the PMP program in promoting adolescent mental health in the school environment and assess the psychological vulnerability of adolescents. Methods: The study was quantitative, observational, descriptive and longitudinal. The sample consisted of 23 students aged 14 to 16 years, and as instrument was applied again the psychological vulnerability scale and the Positive Mental Health Questionnaire (QSM +). Results: Before the program, the class's QSM + was 94.35, after the program it went to 92.61. After 3 months, the QSM + increased to 91.17. Low quotations on the QSM + correspond to higher levels of SMP, ranging from 39 to 156. In EVP, there were changes, going from 14.26 after the program to 15.39 in the follow-up. Conclusions: Although the observed differences have no statistical significance, which allows us to affirm that the PMP Program was effective in the mental health of adolescents and their psychological vulnerability, changes were observed at the behavioral level, with greater mutual help between them, better communication and more active in school activities.

Simple double repetitive element polymerase chain reaction (MaDRE-PCR) and Pvu II-IS1245 restriction fragment length polymorphism (RFLP) typing methods were used to type 41 Mycobacterium avium isolates obtained from 14 Aids inpatients and 10 environment and animals specimens identified among 53 mycobacteria isolated from 237 food, chicken, and pig. All environmental and animals strains showed orphan patterns by both methods. By MaDRE-PCR four patients, with multiple isolates, showed different patterns, suggesting polyclonal infection that was confirmed by RFLP in two of them. This first evaluation of MaDRE-PCR on Brazilian M. avium strains demonstrated that the method seems to be useful as simple and less expensive typing method for screening genetic diversity in M. avium strains on selected epidemiological studies, although with limitation on analysis identical patterns except for one band.