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1.
Use of derived adipose stem cells to reduce complications of cutaneous scarring in smokers. An experimental model in rats
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Martins, João Maximiliano Pedron
; Oliveira, Fernanda dos Santos de
; Lima, Elizabeth Obino Cirne
; Dullius, Diego
; Durli, Isabel Cirne Lima de Oliveira
; Hiraiwa, Everton
; Serrano, Tulio
; Teixeira, Geciele Rodrigues
; Sampaio, Paulo Marcos Ribeiro
; Collares, Marcus Vinicius Martins
.
Abstract Purpose To evaluate the use of adipose-derived stem cells (ADSC) in reducing the necrosis area in an experimental model of cutaneous ischemic flap in rats submitted to subcutaneous nicotine injection to simulate a smoker patient. Methods In an experimental study, 30 rats were enrolled and divided into two experimental groups of 15 animals all submitted to a subcutaneous nicotine injection to create ischemic cutaneous flaps on their backs. Other 10 animals were used only to obtain adipose tissue derived stem cells (ADSC). The first group (n=15) received ADSC treatment at the end of surgery while the other group, the control (n=15), received no other interventions. After euthanasia, a decal was performed on the whole area of the flap, accurately defining the transition from necrosis to healthy region. Photos of all animals were collected and evaluated by scales standardized by Paint-Autocad- 2015 software to define the area of flap necrosis in each rat. Student T test was performed to compare the groups, considering a p< 0.05 significant. Data were analyzed using SPSS IBM® 18 version. Results Through the analysis of the images by the program Paint-Autocad-2015 and the area of decal obtained by the transparent sheet, we obtained a mean of 46% necrosis of the total area of the flap in the treatment group and 69.4% in the control group. In the descriptive analysis, a mean of 3.7 cm of necrosis CI 95% (3.2 - 4.2) was evident in the treatment group whereas a mean value of 5.56 CI 95% (5.2 - 5.9) was found in control group, with p value <0.001 for this comparison. Conclusion The application of adipose-derived stem cells reduces the percentage of necrosis in an experimental model of randomized cutaneous flap in rats submitted to subcutaneous nicotine injection.
https://doi.org/10.1590/s0102-865020190060000005
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2.
Comparação entre as complicações da lipoabdomi-noplastia sem preservação da fáscia de Scarpa com abdominoplastia clássica: um estudo prospectivo cego
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MAXIMILIANO, JOÃO
; OLIVEIRA, ANTONIO CARLOS
; PORTINHO, CIRO PAZ
; FARENZENA, MAURICIO
; REIS, MATHEUS
; SERRANO, TULIO
; DULLIUS, DIEGO
; COLLARES, MARCUS VINICIUS MARTINS
.
▪ ABSTRACT Introduction: Abdominoplasty is among the most commonly performed surgical procedures. Seroma is the most common local complication associated with abdominoplasty, with an average incidence of 10%. The highest incidence of postoperative (PO) seroma occurs on the eleventh postoperative day (POD). Abdominal ultrasound is the method of choice for diagnosing seroma after abdominoplasty. New techniques have emerged aiming to improve aesthetic results with fewer complications, such as lipoabdominoplasty described by Saldanha. However, recent anatomical studies have questioned the need for Scarpa fascia preservation recommended in the lipoabdominoplasty technique, describing that around 90% of the abdominal lymphatic system is in the subdermal plane, while the other 10% is in a deep lymphatic system near the abdominal aponeurosis. The objective is to compare the incidence of seroma in lipoabdominoplasty without Scarpa fascia preservation to that in classic abdominoplasty. Methods: Prospective blinded cohort in which 40 consecutive patients who underwent abdominoplasty without associated liposuction (n = 20) or lipoabdominoplasty (n = 20) at the Hospital de Clínicas of Porto Alegre between April 2016 and May 2017 were analyzed. All patients underwent abdominal wall ultrasonography on the tenth POD. Results: The incidence of seroma was 5% (n = 1) in the classic abdominoplasty group and 10% (n = 2) in the lipoabdominoplasty group, with no statistical difference. Conclusion: These results showed no statistically significant intergroup difference in seroma development.
▪ RESUMO Introdução: Abdominoplastia é um dos procedimentos cirúrgicos estéticos mais realizados. Seroma é a complicação local mais comum associada com abdominoplastia, com uma incidência média de 10%. A maior incidência de seroma pós-operatório (PO) ocorre no décimo primeiro dia PO. Ecografia abdominal é o método de escolha para o diagnóstico de seroma após abdominoplastia. Novas técnicas surgiram ao longo dos anos na tentativa de trazer melhores resultados estéticos com menos complicações, como lipoabdominoplastia descrita por Saldanha. Porém, estudos anatômicos recentes questionam a necessidade da manutenção da fáscia de Scarpa descrita na técnica de lipoabdominoplastia, descrevendo que em torno de 90% do sistema linfático abdominal está no plano subdérmico e 10% em um sistema linfático profundo justa-aponeurose abdominal. O objetivo é comparar a incidência de seroma na lipoabdominoplastia sem preservação da fáscia de Scarpa com a abdominoplastia clássica. Métodos: Coorte prospectiva, cega na qual serão analisados 40 pacientes consecutivos que realizaram abdominoplastia sem lipoaspiração associada (n = 20) ou lipoabdominoplastia (n = 20) no Hospital de Clínicas de Porto Alegre entre abril de 2016 e maio de 2017. Todos foram submetidos à ecografia de parede abdominal no 10o dia PO. Resultados: A incidência de seroma foi de 5% (n = 1) no grupo de abdominoplastia clássica e de 10% (n = 2) no grupo de lipoabdominoplastia, sem diferença estatística. Conclusão: Estes resultados, neste grupo de pacientes, mostram que não houve diferença estatística entre os dois grupos.
3.
Crio-Tomografía de rayos X (Cryo-XT) de fábricas virales en células infectadas con virus vaccinia
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ABSTRACT VV (vaccinia virus) is one of the most complex viruses, with a size greater than 300 nm and more than 100 structural proteins. Its assembly involves sequential interactions and important rearrangements of its structural components. The BSC40 cells were infected and then selected by light fluorescence microscopy and subsequently imaged under the X-ray microscope under cryogenic conditions. Tomographic tilt series of X-ray images were used to produce three-dimensional reconstructions showing different cellular organelles (nuclei, mitochondria, RE), together with two other types of viral particles related to different stages of immature vaccinia (IV) maturation and (MV) mature particles; tests with witaferin showed links with actin, which prevents polymerization and elongation of the filaments; causing poorly packaged or aberrant virions, which inhibits the progression of the viral infection. The findings demonstrate that X-ray cryo-tomography is a powerful tool to collect three-dimensional structural information from frozen whole cells, unfixed and without spots with sufficient resolution to detect different virus particles that exhibit different levels of maturation
RESUMEN El VV (virus vaccinia) es uno de los virus más complejos, con un tamaño superior a 300 nm y más de 100 proteínas estructurales. Su montaje implica interacciones secuenciales y reordenamientos importantes de sus componentes estructurales. Se infectaron las células BSC40 y luego se seleccionaron mediante microscopía de fluorescencia de luz y posteriormente se formaron imágenes en el microscopio de rayos X en condiciones criogénicas. Se utilizaron series de inclinación tomográfica de imágenes de rayos X para producir reconstrucciones tridimensionales que muestran diferentes organelos celulares (núcleos, mitocondrias, RE), junto con otros dos tipos de partículas virales relacionadas con diferentes etapas de maduración del virus vaccinia (IV) inmaduros y (MV) partículas maduras; los ensayos con witaferina mostraron enlaces con actina, que impide la polimerización y el alargamiento de los filamentos; causando viriones mal empaquetados o aberrantes, que inhibe la progresión de la infección viral. Los hallazgos demuestran que la cryo-tomografía de rayos X, es una poderosa herramienta para recolectar información estructural tridimensional a partir de células enteras congeladas, no fijadas y sin manchas con resolución suficiente para detectar diferentes partículas de virus que exhiben distintos niveles de maduración
4.
Trabajos de investigación presentados en el XLIX Congreso Mexicano de Anestesiología: Zacatecas 2015. México
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Sánchez, Claudia
; Osorio-Santiago, María Arelí
; Medina-Tarango, Marco Antonio
; Fernandez De Lara, Alfredo
; Castro-Salinas, Jose Ernesto
; Salazar-Luna, Miguel Angel
; Sanchez, Placido
; Castro-Cayetano, José Esteban
; Millán Hernández, Manuel
; Almonte, Hector Asencion
; Zamora-Aguirre, Salvador A.
; Gudiño, Yocelin Montserrat
; Reyes-Valle, Roberto
; Zepeda-Olivera, Silvia
; Ferretiz-López, Giancarlo
; Salgado, Mónica
; Moctezuma-Ramírez, Luis
; Hernández-Ortiz, Israel Ivan
; Hernández-Zenteno, Norma Angelica
; Martinez, Raquel
; Sánchez-Hernández, Eloy
; Camacho-Gómez, Aldo Constantino
; Martinez, Raquel
; Chenge-Said, Jarumi
; Flores Fonseca, Elvia
; López-Centeno, Martha Alicia
; López, Angélica Yanine
; Jiménez-Ramos, Adriana
; Velasco-Sordo, Luis Ramón
; Hurtado-Reyes, Ignacio Carlos
; Castellanos, Antonio
; Rojas-Peñaloza, Janeth
; Zapién-Madrigal, Jesús Miguel
; Sánchez, Placido
; Sánchez-López, Eric Mauricio
; Mendoza- García, María Isabel
; Calzada-Grijalba, José Francisco
; Hernandez, Israel Ivan
; Castellanos De León, Edgar
; Luna Medina, Cinthya Isabel
; Alvarez-Mora, Lucy Andrea
; López, Angélica Yanine
; Jiménez-Ramos, Adriana
; Velasco-Sordo, Luis Ramón
; Hurtado-Reyes, Ignacio Carlos
; Diaz, Erica Raquel
; Hernandez-Gutierrez, Deoselina
; Gargiulo, Paul Raphael
; Barragán, Maria Jimena
; Ham Mancilla, Ofelia
; Zamora- Gutiérrez, Ana Lilia
; Arteaga, Jaime
; Hernández-Gutierrez, Deoselina
; Ríos-Navarro, Ramón Alejandro
; Romo-Serrano, Erika
; Castellanos, Antonio
; Zapién-Madrigal, Jesús Miguel
; Rojas- Peñaloza, Janeth
; Garduño, Araceli Marselle
; Flores-Rodriguez, Alfonso de Jesus
; Cuervo-Ggonzalez, Alma Kenia
; Puente-Solorio, Armando
; Benitez-Rebollar, Joel Enrique
; Gaona-Valle, Laura Soraya
; Jaime, Mayra Alicia
; Ortega-Suarez, Lizbeth
; Barrientos-Quintanilla, Luis Alberto
; Alvarado-Ramos, Salomon
; Moya-Morales, Yuliana
; Ferretiz, Giancarlo
; César, Julio
; Yañez, Pablo
; Reyes-Valle, Roberto
; Colores-Velazquez, Julio César
; Luna, Arístides De Jesús
; Almonte, Hector Asencion
; Márquez-Noyola, Elizabeth Alicia
; Castellanos, Antonio
; Barrera, Lorena
; Cabrera-Sánchez, Miroslava
; Fernanández-Muñoz, Angel Fernando
; Rolón-Sánchez, Yumara Georgina
; Barragán-Hernández, Adriana Guadalupe
; Lòpez, Estefania
; Osorio-Santiago, Maria Areli
; Muñoz, Beatriz Adelaida
; Cruz-Bautista, Mayra Ivette De La
; Ripoll-Aburto, Carlos Andres
; Ortiz-Maldonado, Jessie Karolina
; Hernández-Rasgado, Javier
; Cuj-Díaz, Alberto
; Sanchez, Placido
; Sarmiento, Lina
; Peña-Olvera, Silvia
; Santiago-Poot, Nohemí
; López, Angélica Yanine
; Jiménez-Ramos, Adriana
; Hurtado-Reyes, Ignacio Carlos
; Ferretiz, Giancarlo
; Pablo-Yañez, Julio César
; Flores-Martinez, Juan Ramón
; Chavez-Contreras, Sara Idali
; Gudiño-Larios, Yocelin Montserrat
; Velazquez, Sara
; Vences-Perez, Noemi Dolores
; Ayala-Bermudez, Celina Alejandra
; Garcia-Castro, Marco Tulio
; Gordillo-Parra, Alejandra
; Barrera, Lorena
; Cabrera-Sánchez, Miroslava
; Martínez-García, Claudia Lizeth
; Zuñiga-Iñiguez, Paloma
; Anaya-Romo, Eduardo
; Antelo-Corral, Jaqueline
; Ramos, Perla Del Carmen
; Hernández-Rasgado, Javier
; Cuj-Díaz, Alberto
; Fonz-Murillo, Elma Patricia
; Cruz-Bautista, Mayra Ivette De la
; Ripoll-Aburto, Carlos Andres
; Cruz, Mayra Ivette De La
; Cuj-Díaz, Alberto
; Hernández-Rasgado, Javier
; Vega-Cano, Miguel
; Ramos-Rodríguez, Perla del Carmen
; Muñoz-Hernández, Beatriz Adelaida
; Caceres, Leonardo Andres
; Arteaga-Favela, Claudia Berenice
; Nava-Osorio, Alejandro
; Arce-Bojorquez, Brisceyda
; Urias, Emma
; Rosario-Ayala, Yuridia Del
; Salazar-Castro, Laura Elena
; Nieblas-Torres, Mariela Lizet
; Arce-Bojorquez, Brisceyda
; Prince-Angulo, Silvia Annel
; Espinoza-Quintero, Erendira
; Alzate, Ana Lizette
; Camacho, Jesus Erick
; Aviles-Lozoya, Jose Trinidad
; Urias, Emma
; Espinoza-Quintero, Erendira
; Castellanos, Antonio
; Cruz, Nora Cecilia
; Rendón-Salazar, Dafne Denisse
; González-Cordero, Gustavo
; Garduño-Chávez, Belia Inés
; Orozco, Arturo Emmanuel
; Guerrero-Morales, Fernando
; Gonzalez-Ortiz, Julio Cesar
; Casillas-Cruz, Angel Alberto
; Aquino-Tamayo, Blanca Olga
; Estrada, Haydeé Lucía
; Soberanes-Alderete, Francisco Javier
; Ojeda-Ramos, Joel
; Martinez-Flores, Carlos
; Sandoval-Rivera, Arturo Guadalupe
; Cáceres-Figeroa, Leonardo Andres
; Ortega, Columba
; Torres-Mata, Lizbeth Yareli
; Peña-Olvera, Silvia
; Huerta, Juana Geraldine
; Sarmiento-Kamar, Rodrigo Ignacio
; Espinoza, Erendira
; Gerardo-Angulo, Alma Ruth
; Nieblas-Torres, Mariela Lizet
; Martinez-Felix, Jesus Israel
; Rochin-Parra, Julia Teresa
; Chavez, Veronica
; Buendía-Roque, Daniel
; Pinales-Aguilar, Guillermina
; Sandoval, Linda Alejandra
; Ovalle-Bueno, Claudia
; Armendáriz-Salinas, Jaime
; Valdovinos, Salvador
; Castellanos, Antonio
; Sandoval, Linda Alejandra
; Medina-Piqué, Sheila
; Aguilar, Hugo
; Barrera, Eduardo
; Márquez, Elizabeth Alicia
; León, Héctor Almonte De
; Castellanos, Antonio
; Rojas-Peñaloza, Janeth
; Zapién-Madrigal, Jesús Miguel
; Paez, Nancy
; Borquez-Torres, Samantha Anabel
; Chinchillas-Chinchillas, Yanira Zelene
; Silva-Romo, Luis Julian
; Aldana, Jorge Pablo
; Hernández-Gutiérrez, Deoselina
; Sánchez-Hernández, Eloy
; Castellanos, Antonio
; Coronel, Berta
; Fuentes-González, Nalleli del Carmen
; Mendoza-García, María Isabel
; Yañez, Gonzalo
; Contreras-Salomón, José Luis
; Garcia-Simbron, Emma
; Chable-Juárez, Randy Josue
; Manriquez-Merino, Ricardo
; Martinez, Raquel
; Chenge-Said, Jarumi
; Hernández Gutiérrez, Deoselina
; Sánchez, Placido
; Arrieta-Ruiz, Marelyn
; González-Velázquez, Felipe
; Estrada-Utrera, Sandra
; Castellanos, Antonio
; Meza, Oscar Eduardo
; Jimenez-Gonzalez, Adriana Guadalupe
; Pilar-Velazquez, Estefania
; Romo-Cortes, Ramona
; Torres-Soto, Maria de la Luz
; Estrada, Maria Sandra
; Espinoza, Cinthia Yadira
; Islas-Ruz, Felix Gilberto
; Perez-Diaz, Roberto
; Manuel-Serna, Roberto Aurelio
; Arce-Bojorquez, Brisceyda
; Medina-Lopez, Francisco de Jesus
; Nieblas-Torres, Mariela Lizet
; Espinoza-Quintero, Erendira
; Ayala-Camargo, Yuridia
; Guadarrama, Hugo
; Flores-Rodriguez, Alfonso de Jesus
; Benitez-Rebollar, Joel Enrique
; Cedillo-Hernandez, Carolina
; Acevedo-Corona, Arturo
; Gaona-Valle, Laura Soraya
; Morfin, Cecilia
; Flores Rodriguez, Alfonso de Jesus
; Cortes-Aceves, Hector Omar
; Rodríguez-Martínez, José Carlos
; Puente Solorio, Armando
; Gaona-Valle, Laura Soraya
; Reyes, Agustin
; Uscanga-Villa, Maria Nieves
; Molina-Rodriguez, Gloria
; Aquino-Tamayo, Blanca Olga
; Cabrera, Francisco
; Motta-Amezquita, Luis Gerardo
; Castellanos-Olivares, Antonio
; Joffre-Mora, Oscar Paul
; Moncada, Adriana Patricia
; Hernandez-Baez, Humberto Trinidad
; Cecilia, Nora
; Garduño-Chávez, Belia Inés
; González-Cordero, Gustavo
; Palacios-Ríos, Dionicio
.
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