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au:SILVA JR, GERALDO A

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Insights into cytochrome bc1 complex binding mode of antimalarial 2-hydroxy-1,4-naphthoquinones through molecular modelling
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Sodero, Ana Carolina Rennó

; Abrahim-Vieira, Bárbara

; Torres, Pedro Henrique Monteiro

; Pascutti, Pedro Geraldo

; Garcia, Célia RS

; Ferreira, Vitor Francisco

; Rocha, David Rodrigues da

; Ferreira, Sabrina Baptista

; Silva Jr, Floriano Paes

Memórias do Instituto Oswaldo Cruz

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apr 2017, Volume 112 N. 4 Pages 299 - 308

Abstract: En | Text: En | PDF: En

BACKGROUND Malaria persists as a major public health problem. Atovaquone is a drug that inhibits the respiratory chain of Plasmodium falciparum, but with serious limitations like known resistance, low bioavailability and high plasma protein binding. OBJECTIVES The aim of this work was to perform molecular modelling studies of 2-hydroxy-1,4-naphthoquinones analogues of atovaquone on the Qo site of P. falciparum cytochrome bc1 complex (Pfbc1) to suggest structural modifications that could improve their antimalarial activity. METHODS We have built the homology model of the cytochrome b (CYB) and Rieske iron-sulfur protein (ISP) subunits from Pfbc1 and performed the molecular docking of 41 2-hydroxy-1,4-naphthoquinones with known in vitro antimalarial activity and predicted to act on this target. FINDINGS Results suggest that large hydrophobic R2 substituents may be important for filling the deep hydrophobic Qo site pocket. Moreover, our analysis indicates that the H-donor 2-hydroxyl group may not be crucial for efficient binding and inhibition of Pfbc1 by these atovaquone analogues. The C1 carbonyl group (H-acceptor) is more frequently involved in the important hydrogen bonding interaction with His152 of the Rieske ISP subunit. MAIN CONCLUSIONS Additional interactions involving residues such as Ile258 and residues required for efficient catalysis (e.g., Glu261) could be explored in drug design to avoid development of drug resistance by the parasite.

https://doi.org/10.1590/0074-02760160417 4132 downloads

Fatores associados à morte materna em unidade de terapia intensiva
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Saintrain, Suzanne Vieira

; Oliveira, Juliana Gomes Ramalho de

; Saintrain, Maria Vieira de Lima

; Bruno, Zenilda Vieira

; Borges, Juliana Lima Nogueira

; Daher, Elizabeth De Francesco

; Silva Jr, Geraldo Bezerra da

Revista Brasileira de Terapia Intensiva

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dec 2016, Volume 28 N. 4 Pages 397 - 404

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo: Identificar os fatores associados à morte materna em pacientes internadas em unidade de terapia intensiva. Métodos: Estudo do tipo transversal realizado em unidade de terapia intensiva materna. Foram selecionados todos os prontuários de pacientes admitidas no período de janeiro de 2012 a dezembro de 2014. O critério de inclusão foi todas as pacientes obstétricas e puérperas, e o de exclusão as com diagnóstico de mola hidatiforme, gravidez ectópica e anembrionada, e as internadas por causas não obstétricas. Foi realizada análise comparativa entre os desfechos óbito e alta hospitalar. Resultados: Foram incluídas 373 pacientes, com idade entre 13 a 45 anos. As causas de internação na unidade de terapia intensiva foram síndromes hipertensivas relacionadas à gestação, cardiopatias, insuficiência respiratória e sepse; as complicações foram lesão renal aguda (24,1%), hipotensão (15,5%), hemorragia (10,2%) e sepse (6,7%). Ocorreram 28 óbitos (7,5%). As causas de óbito foram choque hemorrágico, falência múltipla de órgãos, insuficiência respiratória e sepse. Os fatores de risco independentes para óbito foram lesão renal aguda (OR = 6,77), hipotensão (OR = 15,08) e insuficiência respiratória (OR = 3,65). Conclusão: A frequência de óbitos foi baixa. Lesão renal aguda, hipotensão e insuficiência respiratória foram os fatores de risco independentes associados à mortalidade materna.

ABSTRACT Objective: To identify factors associated with maternal death in patients admitted to an intensive care unit. Methods: A cross-sectional study was conducted in a maternal intensive care unit. All medical records of patients admitted from January 2012 to December 2014 were reviewed. Pregnant and puerperal women were included; those with diagnoses of hydatidiform mole, ectopic pregnancy, or anembryonic pregnancy were excluded, as were patients admitted for non-obstetrical reasons. Death and hospital discharge were the outcomes subjected to comparative analysis. Results: A total of 373 patients aged 13 to 45 years were included. The causes for admission to the intensive care unit were hypertensive disorders of pregnancy, followed by heart disease, respiratory failure, and sepsis; complications included acute kidney injury (24.1%), hypotension (15.5%), bleeding (10.2%), and sepsis (6.7%). A total of 28 patients died (7.5%). Causes of death were hemorrhagic shock, multiple organ failure, respiratory failure, and sepsis. The independent risk factors associated with death were acute kidney injury (odds ratio [OR] = 6.77), hypotension (OR = 15.08), and respiratory failure (OR = 3.65). Conclusion: The frequency of deaths was low. Acute kidney injury, hypotension, and respiratory insufficiency were independent risk factors for maternal death.

https://doi.org/10.5935/0103-507x.20160073 7627 downloads

Recommendations for the treatment of migraine attacks - a Brazilian consensus
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Bordini, Carlos Alberto

; Roesler, Célia

; Carvalho, Deusvenir de Souza

; Macedo, Djacir Dantas P.

; Piovesan, Élcio

; Melhado, Eliana Meire

; Dach, Fabiola

; Kowacs, Fernando

; Silva Júnior, Hilton Mariano da

; Souza, Jano Alves de

; Maciel Jr, Jayme Antunes

; Carvalho, João José de Freitas de

; Speciali, José Geraldo

; Barea, Liselotte Menke

; Queiroz, Luiz Paulo

; Ciciarelli, Marcelo Cedrinho

; Valença, Marcelo Moraes

; Lima, Márcia Maria Ferreira

; Vincent, Maurice Borges

; Jurno, Mauro Eduardo

; Monzillo, Paulo Helio

; Moreira Filho, Pedro Ferreira

; Domingues, Renan

Arquivos de Neuro-Psiquiatria

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mar 2016, Volume 74 N. 3 Pages 262 - 271

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Neste artigo um grupo de especialistas no tratamento de cefaleia da Sociedade Brasileira de Cefaleia através de um consenso elaborou medidas estratérgicas para tratar uma crise de migrânea tanto na criança como no adulto. Uma enfase particular foi dada no tratamento da migranea na mulher, incluindo gravidez, lactação e período perimenstrual.

ABSTRACT In this article, a group of experts in headache management of the Brazilian Headache Society developed through a consensus strategic measurements to treat a migraine attack in both the child and the adult. Particular emphasis was laid on the treatment of migraine in women, including at pregnancy, lactation and perimenstrual period.

https://doi.org/10.1590/0004-282X2015021 10965 downloads

An evaluation of the tolerant to higher inflation rate in the short run by the Brazilian Central Bank in the period 2001-2012
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Moreira, Tito Belchior Silva

; Souza, Geraldo da Silva e

; Ellery Jr, Roberto

Revista Brasileira de Economia

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dec 2013, Volume 67 N. 4 Pages 513 - 528

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Este artigo analisa o grau de tolerância a elevadas taxas de inflação de curto prazo pelos presidentes do Banco Central no período de 2001 a 2012. Foram usados dados mensais e várias especificações e estimativas da regra de Taylor via GMM. Em geral, os resultados mostram as seguintes evidências empíricas: i) o mandato de Henrique Meirelles foi menos tolerante a taxas de inflação mais elevadas do que o mandato de Armínio Fraga e ii) a administração de Alexandre Tombini é mais tolerante a taxas de inflação mais elevadas no curto prazo do que a de Armínio Fraga.

This article investigates the degree of tolerance to higher inflation rates in the short run by the presidents of the Brazilian Central Bank in the period 2001-2012. We used monthly data and various specifications and estimates of the Taylor rule via GMM. In general, the results show the following empirical evidences: i) the mandate of Henrique Meirelles was less tolerant to higher inflation rates when comparing with the mandate of Armínio Fraga and ii) the Alexandre Tombini's administration is more tolerant to higher inflation rate in the short run than Armínio Fraga's.

1302 downloads

Hyponatremia, acute kidney injury, and mortality in HIV-related toxoplasmic encephalitis
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Libório, Alexandre B.

; Silva Jr, Geraldo B.

; Silva, Carolina G.C.H.

; Lima Filho, Francisco J.C.

; Studart Neto, Adalberto

; Okoba, Willy

; Bruin, Veralice M.S. de

; Araújo, Sônia M.H.A.

; Daher, Elizabeth F.

Brazilian Journal of Infectious Diseases

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dec 2012, Volume 16 N. 6 Pages 558 - 563

Abstract: En | Text: En | PDF: En

BACKGROUND: There are no reports on hyponatremia and acute kidney injury (AKI) involved in the course of HIV-related toxoplasmic encephalitis (TE). The main objective of this study was to describe the occurrence of hyponatremia and its relationship with AKI and mortality in HIV-related toxoplasmic encephalitis (TE). METHODS: This was a retrospective cohort study on patients with HIV-related TE. AKI was considered only when the RIFLE (risk, injury, failure, loss, end-stage) criterion was met, after the patient was admitted. RESULTS: A total of 92 patients were included, with a mean age of 36 ± 9 years. Hyponatremia at admission was observed in 43 patients (46.7%), with AKI developing in 25 (27.1%) patients during their hospitalization. Sulfadiazine was the treatment of choice in 81% of the cases. Death occurred in 13 cases (14.1%). Low serum sodium level correlated directly with AKI and mortality. Male gender (OR 7.89, 95% CI 1.22-50.90, p = 0.03) and hyponatremia at admission (OR 4.73, 95% CI 1.22-18.30, p = 0.02) were predictors for AKI. Independent risk factors for death were AKI (OR 8.3, 95% CI 1.4-48.2, p < 0.0001) and hyponatremia (or 9.9, 95% ci 1.2-96.3, p < 0.0001). CONCLUSION: AKI and hyponatremia are frequent in TE. Hyponatremia on admission is highly associated with AKI and mortality.

https://doi.org/10.1016/j.bjid.2012.08.015 2252 downloads

Abordagem discreta para a dinâmica da taxa Selic-meta
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Caetano, Sidney Martins

; Silva Jr, Geraldo Edmundo

; Corrêa, Wilson Luiz Rotatori

Economia Aplicada

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jun 2011, Volume 15 N. 2 Pages 199 - 221

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O presente artigo utiliza abordagem discreta não estacionaria para modelar taxa de juros no Brasil. O diferencial está em abordar o problema em termos das decisões de alta, baixa ou manutenção da taxa Selic tomadas pelo Copom como uma decisão discreta ao invés de contínua. O modelo proposto se mostra parcimonioso e capaz de prever 83% das decisões do Copom. O modelo permite que os parâmetros estimados que definem o limiar para o qual o Copom tomaria a decisão sejam assimétricos, mas não encontra diferenças significativas entre os limiares de alta e baixa.

In this paper we do follow a non-stationary discrete approach to model the baseline rate in Brazil. The paper innovates in approaching the decisions in terms of a hike, maintenance or cut in the baseline rate rather than assume that it follows a continuous path. Our model seems to be parsimonious and forecast correctly 83% of the actual Copom decisions. The estimated parameters define a threshold to the Copom actions which is asymmetric, but finds no significant differences between high and low thresholds.

7230 downloads

Comparação dos valores da capacidade pulmonar total e do volume residual obtidos pelas técnicas pletismográfica e de respiração única com metano
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Dias, Ricardo Marques

; Chacur, Fernando Hauaji

; Carvalho, Sonia Regina da Silva

; Mancini, André Luís

; Capuchino Jr, Geraldo A

Revista Portuguesa de Pneumologia

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nov 2006, Volume 12 N. 6 Pages 659 - 667

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Foram analisados exames de função pulmonar de 20 asmáticos, em acompanhamento no HU Gaffrée Guinle, classificados, segundo o Consenso Brasileiro (2002), em asma leve persistente ou moderada (9) e grave (11). Foram obtidos os valores dos parâmetros da espirografia, da pletismografia e da técnica de respiração única, com metano, para a medida da difusão pulmonar (DLco). Assim, a capacidade pulmonar total e o volume residual, quando medidos pela pletismografia, foram designados, respectivamente, CPTPL e VRPL. Da mesma forma, quando determinados pela técnica de respiração única, CPTSB e VRSB. A amostra foi constituída por 13 mulheres e 7 homens, sendo a média de idade de 47,6 anos. A intensidade da disfunção, medida pela relação VEMS/CVF foi de 58,8%, com IC95 de 53,9 a 63,6. Os valores de CPTPL (5,94) e VRPL (2,55), expressos em litros e pela média, foram significativamente maiores do que os de CPTSB (4,73) e VRSB (1,66). Foram determinadas equações de regressão múltipla, para previsão dos valores de CPTPL e VRPL, utilizando os valores de CPTSB, VRSB e dos parâmetros espirográficos, com coeficientes de regressão significativos. Contudo, a inclusão dos parâmetros espirográficos, excepto por CVF, não melhorou a capacidade de previsão das equações. Considerando apenas a CPTSB, r2=0,79, a equação é: CPTPL=(CPTSB*1,025)+1,088, com EP=0,64. A regressão para VRPL, com r2=0,23, é: VRPL=(VRSB*0,9268)+1,012. Os exames realizados após a broncodilatação, com 400mcg de salbutamol, não melhoraram as equações. Concluímos que a técnica de SB não dá resultados iguais aos da pletismografia para CPT e VR; contudo, para a CPT, esta diferença pode ser prevista.

We analyzed pulmonary function tests of twenty asthmatic patients from Gaffrée e Guinle University Hospital, classified according to Brazilian Guidelines for Asthma (2002), similar to GINA, into mild persistent or moderate (9) or severe (11) asthma. We obtained parameters from spirometry, plethysmograph(PL) and single breath technique for diffusion capacity (SB), with methane. Total lung capacity and residual volume were called TLCPL and RVPL when measured by pletysmography and TLCSB and RVSB when determined by single breath test. There were 13 women and 7 men with mean age of 47.6 years. The pulmonary dysfunction degree to FEV1/FVC was 58.8% with CI95=53.9 to 63.6. The mean values in litres for TLCPL (5.94) and RVPL (2.55) were significantly higher than for TLCSB (4.73) and RVSB (1.66). Multiple regression equations were determined for TLCPL e RVPL using only single breath values,TLCSB or RVSB, and spirographic parameters, with significant regression coefficients. However, the inclusion of spirometric parameters, except for FVC, did not improve the predicted capacity for the equations. Considering only the TLCSB, r2=0.79, the equation is: TLCPL=(TLCSB*1.025)+1.088, with EPE=0.64. The regression for RVPL, r2=0.23, is: RVPL=(RVSB*0.9268)+1.012. The results obtained after bronchodilation with 400 mcg of salbutamol did not improve the regression. We concluded that the SB technique did not obtain the same results as pletysmography for TLC and RV, but for TLC this difference can be predicted.

1338 downloads

Efeito de diferentes temperaturas no desenvolvimento e reprodução de Tetranychus abacae Baker & Printchard (Acari: Tetranychidae) em bananeira Musa sp. cv. Prata
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Vasconcelos, Geraldo J.N.

; Silva, Fernando R. da

; Gondim Jr, Manoel G.C.

; Barros, Reginaldo

; Oliveira, Jose V.

Neotropical Entomology

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apr 2004, Volume 33 N. 2 Pages 149 - 154

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Estudaram-se as exigências térmicas de Tetranychus abacae Baker & Printchard em Musa sp. cv. Prata em quatro temperaturas constantes (20; 23; 25,5 e 30°C), bem como o efeito dessas sobre a fase adulta. Com esses dados, foram construídas tabelas de vida de fertilidade. Os estágios de ovo, larva, protoninfa, deutoninfa e ovo-adulto apresentaram temperatura base de 14,9; 15,2; 14,2; 16,2 e 15,3°C, respectivamente. As constantes térmicas foram de 47,7; 25,4; 25,5; 27,4 e 124,3 GD para as fases de ovo, larva, protoninfa, deutoninfa e período de ovo-adulto, respectivamente. A maior fecundidade foi verificada na temperatura de 25,5°C. A duração média da geração (T) variou de 50,03 a 13,51 dias entre as temperaturas de 20ºC e 30°C. A taxa líquida de reprodução (R0) foi maior na temperatura de 25,5°C, aumentando 28,7 vezes a cada geração. A capacidade reprodutiva (r m) e a razão finita de aumento (l) variaram de 0,049 e 1,05 a 0,226 e 1,25 entre as temperaturas de 20ºC a 30°C, respectivamente. Estimou-se que T. abacae pode desenvolver até 31 gerações por ano a 26°C em condições de campo e 43 gerações por ano a 30°C em condições de casa de vegetação, no estado de Pernambuco.

The thermal requirements of Tetranychus abacae Baker & Printchard in Musa sp. cv. Prata were studied at constant temperatures (20; 23; 25,5 and 30°C), as well as the effect of the temperatures on the adult phase. Age-specific life tables were built with the data. The basal temperature and thermal constant for egg, larva, protonymph, deutonymph and egg-adult development were: 14,9; 15,2; 14,2; 16,2 and 15,3°C and 47,7; 25,4; 25,5; 27,4 and 124,3 GD, respectively. High fecundity was registered at 25,5°C. The mean duration of the generation (T) varied from 50,03 to 13,51 days between 20ºC and 30°C. The net reproductive rate (R0) was high at the temperature of 25,5°C, increasing 28,7 times at each generation. The intrinsic rate of natural increase (r m) and the finite rate of increase (l) varied from 0,049 and 1,05 to 0,226 and 1,25 between 20ºC to 30°C, respectively. It as possible to estimate that T. abacae can develop up to 31 generations per year at 26°C in field conditions and 31 generations per year at 30°C in green house conditions, in Pernambuco State, Brazil.

1963 downloads

Etiology of parkinsonism in a Brazilian movement disorders clinic
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CARDOSO, FRANCISCO

; CAMARGOS, SARAH T

; SILVA JR, GERALDO A

Arquivos de Neuro-Psiquiatria

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jun 1998, Volume 56 N. 2 Pages 171 - 175

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVO: O objetivo deste estudo é investigar se há diferenças geográficas na etiologia de parkinsonismo (PA). PANORAMA: 72% dos pacientes com PA avaliados em Clínicas de Distúrbios do Movimento no hemisfério norte são diagnosticados com doença de Parkinson (DP). Dados a respeito de outras regiões não se encontram disponíveis. MÉTODOS: Nós revisamos os prontuários de todos pacientes com PA vistos na Clínica de Distúrbios de Movimentos da Universidade Federal de Minas Gerais entre Julho 1993 e outubro 1995. PA foi diagnosticado pela presença de no mínimo dois dos seguintes: tremor de repouso, bradicinesia, rigidez e instabilidade postural. As diferentes etiologias foram diagnosticadas baseadas em critérios clínicos padrões RESULTADOS: Durante o período do estudo, PA foi reconhecido em 338 indivíduos. Os seguintes diagnósticos clinícos foram feitos: DP (68,9%), PA induzido por droga (PID) (13,3%), PA vascular (4,7%), paralisia supranuclear progressiva (PSP) (2%), atrofia de múltiplos sistemas (AMS) (1,8%), outros (9,7%). Cinarizina, haloperidol e flunarizina foram as drogas mais comumente relacionadas a PID. CONCLUSÕES: À semelhança de outros estudos, DP é responsável por cerca de 70% dos casos de PA. Existem, porém, diferenças entre nossos resultados e outras séries. PID é muito mais comum na população estudada. Isso pode ser explicado por uso mais liberal de drogas antidopaminérgicas no nosso meio, sobretudo bloqueadores de canal de Calcio. A baixa frequência de AMS e PSP no nosso estudo pode refletir tempo de seguimento curto, já que muitos pacientes com diagnóstico inicial de DP posteriormente desenvolvem síndrome Parkinson-plus.

OBJECTIVE: The aim of the present study is to investigate whether there are geographic differences in the etiology of parkinsonism (PA). BACKGROUND: 72% of patients with PA evaluated at movement disorders clinics in the Northern Hemisphere are diagnosed with Parkinson's disease (PD). Data regarding other regions are not available. METHODS: We reviewed the charts of all patients with PA seen at the Federal University of Minas Gerais Movement Disorders Clinic from July 1993 through October 1995. PA was diagnosed by the presence of at least two of the following: rest tremor, bradykinesia, rigidity, and postural instability. The different etiologies were diagnosed based on standard clinical criteria RESULTS: During the period of the study, PA was recognized in 338 subjects. The following clinical diagnoses were made: PD (68.9%), drug-induced PA (DIP) (13.3%), vascular PA (4.7%), Progressive supranuclear palsy (PSP) (2%), multiple system atrophy (MSA) (1.8%), others (9.7%). Cinnarizine, haloperidol and flunarizine were the commonest drugs related to DIP. CONCLUSIONS: Similarly to other studies, PD accounts for about 70% of PA patients. However, there are differences between our results and previous series. DIP is much more common in the present series. This may be accounted for a more liberal use of antidopaminergic drugs in our environment, especially Calcium channel blockers. The lower frequency of MSA and PSP in our study may reflect a short follow-up, since many patients initially diagnosed with PD later are found to have Parkinson-plus syndromes.

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