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au:SILVA, ANA CRISTINA S. E

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Biochemical parameters, C-reactive protein, and proteinogram of Sapajus libidinosus kept in captivity
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Ferreira, Adriano Fernandes

; Silva, Grasiene Meneses

; Silvestre-Ferreira, Ana Cristina

; Queiroga, Felisbina Luísa

Ciência Rural

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2023, Volume 53 N. 6 elocation e20210789

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: O Sapajus libidinosus é um primata do novo mundo pertencente à família Cebidae que habita os biomas da caatinga e cerrado do Brasil. Atualmente esta classificado como quase ameaçado pelos principais orgãos de proteção animal. O conhecimento da bioquímica sérica, proteína C reativa e proteinograma são importantes na preservação desta espécie. O nosso estudo tem como objetivo determinar os intervalos de referência para várias variáveis bioquímicas e proteinograma. Para isso, foram colhidas amostras de sangue de 50 macacos da espécie S. libidinosus (25 machos e 25 fêmeas) mantidos em cativeiro no estado da Paraíba, Brasil. A análise descritiva e os cálculos dos intervalos de referência foram estimados segundo as diretrizes da Sociedade Americana de Patologia Clínica Veterinária com o uso do software Reference Value Advisor 2.1. Na população total (n=50), considerando o factor idade, foram observadas diferenças significativas (P < 0,05) para a creatinina e proteínas totais (valores superiores nos adultos) e para a albumina e bilirrubina indirecta (valores superiores nos jovens). Foram encontradas diferenças associadas ao sexo (fêmeas versus machos, P < 0,05) para a ureia e creatinina séricas (valores superiores nos machos) e albumina e Beta-Globulinas (valores superiores nas fêmeas). Conclui-se que, tanto quanto é do conhecimento dos autores, os resultados deste estudo são inovadores e podem ser utilizados como intervalos de referência para avaliação do estado de higidez dos animais e é pioneiro na determinação da proteína C reativa nesta espécie. É importante salientar que o sexo e a faixa etária podem ter influência e devem ser levados em consideração pelos profissionais na interpretação dos exames.

ABSTRACT: Sapajus libidinosus is a New World primate belonging to the Cebidae family that lives in the caatinga and cerrado, which are known Brazilian biomes. It is currently classified as near threatened, almost endangered, by the main animal protection organizations. Knowledge of biochemistry, the C-reactive protein, and the proteinogram are important for the preservation of this species. Our study established reference intervals for several biochemical variables and the proteinogram. For this purpose, blood samples were collected from 50 S. libidinosus monkeys (25 males and 25 females) kept in captivity at the Brazilian state of Paraíba. Descriptive analysis and reference intervals were estimated following the guidelines of the American Society of Veterinary Clinical Pathology, using the Reference Value Advisor 2.1 software. In the overall population (n=50), significant differences (P < 0.05) were noted for creatinine and total proteins when considering the age factor (higher in adults), as well as for albumin and indirect bilirubin (higher in juveniles). Sex-associated differences (females versus males, P < 0.05) were reported for serum urea and creatinine (higher in males), as well as for albumin and Beta-Globulins (higher in females). In conclusion, to the authors’ best knowledge, the present results are innovative and can be used as reference intervals for assessing the animals’ health status. Moreover, it is also pioneer in determining the C-reactive protein in this species. It is important to emphasize that gender and age categories can have an influence and should be considered when interpreting the tests results.

https://doi.org/10.1590/0103-8478cr20210789

Expression of TIGIT, PD-1 and HLA-DR/CD38 markers on CD8-T cells of children and adolescents infected with HIV and uninfected controls
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Pereira-Manfro, Wânia Ferraz

; Silva, Giselle Pereira da

; Costa, Priscilla Ramos

; Costa, Dayane Alves

; Ferreira, Bianca da Silva

; Barreto, Daniela Mena

; Frota, Ana Cristina Cisne

; Hofer, Cristina Barroso

; Kallas, Esper Georges

; Milagres, Lucimar Gonçalves

Revista do Instituto de Medicina Tropical de São Paulo

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2023, Volume 65 elocation e14

Abstract: En | Text: En | PDF: En

ABSTRACT Immune exhaustion and senescence are scarcely studied in HIV-pediatric patients. We studied the circulatory CD8 T cells activation/exhaustion and senescent phenotype of children and adolescents vertically infected with HIV or uninfected controls based on the expression of human leukocyte antigen (HLA-DR), CD38, T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif (ITIM) domain (TIGIT), programmed death 1 (PD-1) and CD57 by flow cytometry, during approximately one year. Eleven HIV-infected (HI) and nine HIV-uninfected (HU) children/adolescents who received two doses or one dose of meningococcal C conjugate vaccine (MenC), respectively, were involved in this study. Blood samples were collected before the immunization (T0), 1–2 months after the first dose (T1), and 1–2 months after the second dose (T2), which was administered approximately one year after the first one. HI patients not receiving combined antiretroviral therapy (cART) showed a higher frequency of CD8 T cells TIGIT+, PD-1+ or CD57+, as well as a higher frequency of CD8 T cells co-expressing CD38/HLA-DR/TIGIT or CD38/HLA-DR/PD-1 when compared to HI treated or HU individuals, at all times that they were assessed. CD8 T cells co-expressing CD38/DR/TIGIT were inversely correlated with the CD4/CD8 ratio but positively associated with viral load. The co-expression of CD38/DR/TIGIT or CD38/DR/PD-1 on CD8 T cells was also inversely associated with the CD4 T cells expressing co-stimulatory molecules CD127/CD28. The results showed a higher expression of exhaustion/senescence markers on CD8 T cells of untreated HI children/adolescents and its correlations with viral load.

https://doi.org/10.1590/s1678-9946202365014

Assessment of the microbiological quality and safety in takeaway sushi meals in Portugal
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Alegria, Sandy J.C.

; Santos, Maria Isabel S.

; Furtado, Rosália M.S.

; Correia, Cristina Belo

; Lima, Ana Isabel G.

; Pedroso, Laurentina R.

; Ramos, Sónia Catarina da Silva

Portuguese Journal of Public Health

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aug 2022, Volume 40 N. 2 Pages 69 - 80

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract Being a food product that contains perishable ingredients and involves a significant degree of manual handling during preparation, sushi is regarded as a potentially hazardous food, which may lead to foodborne disease outbreaks. In Portugal, consumption of takeaway sushi meals has strongly increased throughout the past few years; however, there is limited information regarding its compliance with food quality standards. Under this context, the present study aimed to evaluate the microbiological quality and safety of take-away ready-to-eat sushi meals in Lisbon, Portugal. Sixty-two samples were collected from different origins (restaurant and hypermarket), and each sample was tested for aerobic mesophilic microorganisms, Enterobacteriaceae, Escherichia coli, positive coagulase Staphylococci, presumptive Bacillus cereus count, as for detection of pathogenic microorganisms, such as Salmonella spp., Listeria monocytogenes and Vibrio parahaemolyticus, V. cholerae and V. vulnificus. Results revealed that 48.4% (30/62) were deemed unsatisfactory, 35.5% (22/62) were classified as borderline and only 16.1% (10/62) were considered satisfactory. Even though we did not detect the incidence of potentially pathogenic microorganisms in sushi, the presence of B. cereus and coagulase-positive Staphylococci was detected at unsatisfactory levels. Furthermore, significant differences between the place of origin (restaurant vs. hypermarket) and type of fish were also observed. Overall, the high number of samples classified with a level of microbiological quality “unsatisfactory” and “borderline” highlights the need to review good hygiene practices, as well as the quality of the raw materials used, to obtain a final product with a satisfactory quality and safety level.

Resumo Por ser um género alimentício que contém ingredientes perecíveis e envolve um grau significativo de manipulação manual durante a sua preparação, o sushi é considerado um alimento potencialmente perigoso, que pode causar surtos de doença de origem alimentar. Em Portugal, o consumo de refeições de sushi prontas a consumir tem aumentado ao longo dos últimos anos. No entanto, a informação sobre o cumprimento das normas de qualidade alimentar é limitada. Neste contexto, o presente estudo teve como objetivo avaliar a qualidade e a segurança microbiológica de refeições de sushi prontas para consumo em take-way, na região de Lisboa, Portugal. Foram colhidas 62 amostras de diferentes origens (restaurante e hipermercado), e em cada amostra foi efetuada a contagem de microrganismos aeróbios mesófilos, Enterobacteriaceae, Escherichia coli, estafilococos coagulase positiva, Bacillus cereus presuntivos, e deteção de microrganismos patogénicos, tais como: Salmonella spp., Listeria monocytogenes, Vibrio parahaemolyticus, Vibrio cholerae e Vibrio vulnificus. Os resultados revelaram que 48,4% (30/62) das amostras foram consideradas insatisfatórias, 35,5% (22/62) foram classificadas como “borderline” e apenas 16,1% (10/62) foram consideradas como satisfatórias. Embora não tenham sido detetados microrganismos potencialmente patogénicos nas amostras de sushi, a presença de B. cereus e estafilococos coagulase positivos foram detetados em níveis insatisfatórios. Além disso, também foram observadas diferenças significativas entre o local de origem (restaurante vs. hipermercado) e tipo de peixe.No geral, o elevado número de amostras classificadas com um nível de qualidade microbiológica insatisfatória e “borderline” evidência a necessidade de revisão das boas práticas de higiene, bem como da qualidade das matérias-primas utilizadas, para obter um produto final com qualidade e segurança satisfatória.

https://doi.org/10.1159/000525005

Microbiological evaluation of industrialized and artisanal Minas fresh cheese commercialized in the Federal District, Brazil
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FERNANDES SILVA RODRIGUES, Letícia

; LUNARA SANTOS PAVELQUESI, Sabrina

; ALMEIDA DE OLIVEIRA FERREIRA, Ana Carolina

; DA SILVA MONTEIRO, Erika

; DE SOUZA SILVA, Calliandra Maria

; RODRIGUES DA SILVA, Izabel Cristina

; CASTILHO ORSI, Daniela

Food Science and Technology

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2022, Volume 42 elocation e45221

Abstract: En | Text: En | PDF: En

Abstract The aim of this study was to evaluate the microbiological quality of Minas fresh cheese commercialized in Federal District, Brazil. For this, 20 samples of industrialized cheeses were collected in supermarkets and 20 samples of artisanal cheeses were collected in public food markets. The analyzes performed were total count of bacteria, determination of coliforms, count of S. aureus and identification of Salmonella spp. (InvA gene) and S. aureus (SeC gene). The results showed that 26 samples (65%) were unfit for consumption according to Brazilian legislation. For industrialized cheeses, 6 samples (15%) of were unfit for consumption (3 samples for excess of thermotolerant coliforms and 3 samples for excess of S. aureus). For artisanal cheeses, all 20 samples (50%) were unfit for consumption due to the high S. aureus counts, including 6 samples that had excess thermotolerant coliforms and 1 sample that was contaminated with Salmonella. Comparing the results, industrialized cheeses showed better microbiological quality than artisanal cheeses. Thus, artisanal Minas fresh cheeses commercialized in the public food markets of the Federal District showed the presence of bacterial hazards, and there is an evident need for good hygiene practices in the entire production chain to ensure consumer food safety.

https://doi.org/10.1590/fst.45221

Chronic inflammatory diseases, subclinical atherosclerosis, and cardiovascular diseases: Design, objectives, and baseline characteristics of a prospective case-cohort study ‒ ELSA-Brasil
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Bensenor, Isabela M.

; Goulart, Alessandra C.

; Pereira, Alexandre C.

; Brunoni, André R.

; Alencar, Airlane

; Santos, Raul D.

; Bittencourt, Márcio S.

; Telles, Rosa W.

; Machado, Luciana Andrade Carneiro

; Barreto, Sandhi Maria

; de Almeida-Pititto, Bianca

; Janovsky, Carolina Porto Silva

; Sgarbi, José Augusto

; Tebar, William R.

; Meneghini, Vandrize

; Barbosa Junior, Fernando

; Ribeiro, Ana Cristina de Medeiros

; Pasoto, Sandra Gofinet

; Pereira, Rosa Maria R.

; Bonfá, Eloísa

; Sipahi, Aytan M.

; Santos, Itamar de S.

; Lotufo, Paulo A.

Clinics

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2022, Volume 77 elocation 100013

Abstract: En | Text: En | PDF: En

Abstract Objectives This analysis describes the protocol of a study with a case-cohort to design to prospectively evaluate the incidence of subclinical atherosclerosis and Cardiovascular Disease (CVD) in Chronic Inflammatory Disease (CID) participants compared to non-diseased ones. Methods A high-risk group for CID was defined based on data collected in all visits on self-reported medical diagnosis, use of medicines, and levels of high-sensitivity C-Reactive Protein >10 mg/L. The comparison group is the Aleatory Cohort Sample (ACS): a group with 10% of participants selected at baseline who represent the entire cohort. In both groups, specific biomarkers for DIC, markers of subclinical atherosclerosis, and CVD morbimortality will be tested using weighted Cox. Results The high-risk group (n = 2,949; aged 53.6 ± 9.2; 65.5% women) and the ACS (n=1543; 52.2±8.8; 54.1% women) were identified. Beyond being older and mostly women, participants in the high-risk group present low average income (29.1% vs. 24.8%, p < 0.0001), higher BMI (Kg/m2) (28.1 vs. 26.9, p < 0.0001), higher waist circumference (cm) (93.3 vs. 91, p < 0.0001), higher frequencies of hypertension (40.2% vs. 34.5%, p < 0.0001), diabetes (20.7% vs. 17%, p = 0.003) depression (5.8% vs. 3.9%, p = 0.007) and higher levels of GlycA a new inflammatory marker (p < 0.0001) compared to the ACS. Conclusions The high-risk group selected mostly women, older, lower-income/education, higher BMI, waist circumference, and of hypertension, diabetes, depression, and higher levels of GlycA when compared to the ACS. The strategy chosen to define the high-risk group seems adequate given that multiple sociodemographic and clinical characteristics are compatible with CID.

https://doi.org/10.1016/j.clinsp.2022.100013

Consenso de leucemia mieloide aguda en México
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Arana-Luna, Luara L.

; Alvarado-Ibarra, Martha

; Silva-Michel, Luis G.

; Morales-Maravilla, Adrián

; González-Rubio, María del C.

; Chávez-Aguilar, Lénica A.

; Tena-Iturralde, María Fernanda

; Mojica-Balceras, Liliana

; Zapata-Canto, Nidia

; Galindo-Delgado, Patricia

; Miranda-Madrazo, María Raquel

; Morales-Hernández, Alba E.

; Silva-Vera, Karina

; Grimaldo-Gómez, Flavio A.

; Hernández-Caballero, Álvaro

; Bates-Martin, Ramón A.

; Álvarez-Vera, José L.

; Tepepa-Flores, Fredy

; Teomitzi-Sánchez, Óscar

; Fermín-Caminero, Denisse J.

; Peña-Celaya, José A. de la

; Salazar-Ramírez, Óscar

; Flores-Villegas, Luz V.

; Guerra-Alarcón, Lidia V.

; Leyto-Cruz, Faustino

; Inclán-Alarcón, Sergio I.

; Milán-Salvatierra, Andrea I.

; Ventura-Enríquez, Yanet

; Pérez-Lozano, Uendy

; Báez-Islas, Pamela E.

; Tapia-Enríquez, Ana L.

; Palma-Moreno, Orlando G.

; Aguilar-Luévano, Jocelyn

; Espinosa-Partida, Arturo

; Pérez-Jacobo, Luis F.

; Rojas-Castillejos, Flavio

; Ruiz-Contreras, Josué I.

; Loera-Fragoso, Sergio J.

; Medina-Coral, Jesús E.

; Acosta-Maldonado, Brenda L.

; Soriano-Mercedes, Emely J.

; Saucedo-Montes, Erick E.

; Valero-Saldana, Luis M.

; González-Prieto, Susana G.

; Nava-Villegas, Lorena

; Hernández-Colin, Ana K.

; Hernández-Alcántara, Areli E.

; Zárate-Rodríguez, Pedro A.

; Ignacio-Ibarra, Gregorio

; Meillón-García, Luis A.

; Espinosa-Bautista, Karla A.

; Ledesma de la Cruz, Cindy

; Barbosa-Loría, Diego M.

; García-Castillo, Carolina

; Balderas-Delgado, Carolina

; Cabrera-García, Álvaro

; Pérez-Zúñiga, Juan M.

; Hernández-Ruiz, Eleazar

; Villela-Peña, Atenas

; Gómez Cortés, Sue Cynthia

; Romero-Rodelo, Hilda

; Garzón-Velásquez, Katheryn B.

; Serrano-Hernández, Cristina

; Martínez-Ríos, Annel

; Pedraza-Solís, María Luisa

; Martínez-Coronel, Jorge A.

; Narváez-Davalos, Iris M.

; García-Camacho, Alinka S.

; Merino-Pasaye, Laura E.

; Aguilar-Andrade, Carolina

; Aguirre-Domínguez, Juan A.

; Guzmán-Mera, Pedro G.

; Delgado-de la Rosa, Elizabeth

; Flores López, Perla E.

; González-Aguirre, Lilia L.

; Ramírez-Alfaro, Edgar M.

; Vera-Calderón, Heidi

; Meza-Dávalos, María Lizeth

; Murillo-Cruz, Juan

; Pichardo-Cepín, Yayra M.

; Ramírez-Romero, Eva F.

Gaceta médica de México

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2022, Volume 158 N. spe3 Pages M1 - M51

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Abstract Acute myeloid leukemia (AML) comprises a heterogeneous group of hematopoietic cell neoplasms of myeloid lineage that arise from the clonal expansion of their precursors in the bone marrow, interfering with cell differentiation, leading to a syndrome of bone marrow failure. AML is a consequence of genetic and epigenetic changes (point mutations, gene rearrangements, deletions, amplifications, and arrangements in epigenetic changes that influence gene expression) in hematopoietic precursor cells, which create a clone of abnormal cells that are capable of proliferating but cannot differentiate into mature hematopoietic cells or undergo programmed cell death. The diagnosis requires more than 20% myeloid blasts in the bone marrow and certain cytogenic abnormalities. Treatment will depend on age, comorbidities, and cytogenetic risk among the most frequent.

resumen está disponible en el texto completo

https://doi.org/10.24875/gmm.m21000597 895 downloads

Biological properties of Schinus terebinthifolia Raddi essential oil
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Vasconcelos, Priscilla Guimarães Silva

; Alves, Érika Ponchet

; Maia, Carolina Medeiros de Almeida

; Brito, Arella Cristina Muniz

; Silva, Diego Romário

; Freires, Irlan Almeida

; Cavalcanti, Yuri Wanderley

; Rehder, Vera Lucia Garcia

; Ruiz, Ana Lúcia Tasca Gois

; Duarte, Marta Cristina Teixeira

; Rosalen, Pedro Luiz

; Godoy, Gustavo Pina

; Costa, Edja Maria Melo de Brito

Brazilian Journal of Pharmaceutical Sciences

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2022, Volume 58 elocation e20417

Abstract: En | Text: En | PDF: En

Abstract Schinus terebinthifolia Raddi green fruits essential oil (EO) was evaluated regarding its phytochemical profile, antimicrobial and cytotoxic activities, and toxicity. Gas chromatography with mass spectrometry was applied to identify its constituents, thereafter the minimum inhibitory concentration, minimum bactericidal and fungicidal concentrations, and its antibiofilm activity were evaluated. The EO cytotoxicity was assessed in tumor and non-tumor human cells, and in vivo toxicity was evaluated in a Galleria mellonella model. The major constituents of S. terebinthifolia EO were alpha-phellandrene and beta-phellandrene. The EO had a weak activity against all strains of Candida albicans (MIC 1000μg/mL) and had no activity against non-albicans strains, bacteria, and C. albicans biofilm. Cytostatic activity against all tumor cell lines was shown. Additionally, cell viability remained at EO concentrations up to 62.5 μg/mL. At 16 mg/mL, 50% hemolysis was observed, and it had low toxicity in vivo. Overall, the S. terebinthifolia EO was characterized by low antimicrobial and antibiofilm activities, with no evidence of toxicity to human tumor and non-tumor cells.

https://doi.org/10.1590/s2175-97902021000x2e20417

68Ga-Prostate-specific membrane antigen (PSMA) positron emission tomography (pet) in prostate cancer: a systematic review and meta-analysis
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Matushita, Cristina S.

; Silva, Ana M. Marques da

; Schuck, Phelipi N.

; Bardisserotto, Matteo

; Piant, Diego B.

; Pereira, Jonatas L.

; Cerci, Juliano J.

; Coura Filho, George B.

; Esteves, Fabio P.

; Amorim, Barbara J.

; Gomes, Gustavo V.

; Brito, Ana Emília T.

; Bernardo, Wanderley M.

; Mundstock, Eduardo

; Fanti, Stefano

; Macedo, Bruna

; Roman, Diego H.

; Tem-Pass, Cinthia Scatolin

; Hochhegger, Bruno

International braz j urol

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aug 2021, Volume 47 N. 4 Pages 705 - 729

Abstract: En | Text: En | PDF: En

ABSTRACT Introduction: Prostate cancer (PC) is the second most commonly diagnosed cancer in males. 68Ga-PSMA PET/CT, a non-invasive diagnostic tool to evaluate PC with prostate-specific membrane antigen (PSMA) expression, has emerged as a more accurate alternative to assess disease staging. We aimed to identify predictors of positive 68Ga-PSMA PET and the accuracy of this technique. Materials and methods: Diagnostic accuracy cross-sectional study with prospective and retrospective approaches. We performed a comprehensive literature search on PubMed, Cochrane Library, and Embase database in search of studies including PC patients submitted to radical prostatectomy or radiotherapy with curative intent and presented biochemical recurrence following ASTRO 1996 criteria. A total of 35 studies involving 3910 patients submitted to 68-Ga-PSMA PET were included and independently assessed by two authors: 8 studies on diagnosis, four on staging, and 23 studies on restaging purposes. The significance level was α=0.05. Results: pooled sensitivity and specificity were 0.90 (0.86-0.93) and 0.90 (0.82-0.96), respectively, for diagnostic purposes; as for staging, pooled sensitivity and specificity were 0.93 (0.86-0.98) and 0.96 (0.92-0.99), respectively. In the restaging scenario, pooled sensitivity and specificity were 0.76 (0.74-0.78) and 0.45 (0.27-0.58), respectively, considering the identification of prostate cancer in each described situation. We also obtained specificity and sensitivity results for PSA subdivisions. Conclusion: 68Ga-PSMA PET provides higher sensitivity and specificity than traditional imaging for prostate cancer.

https://doi.org/10.1590/s1677-5538.ibju.2019.0817 81 downloads

Prevalence of nasal carriers of methicillin-resistant Staphylococcus aureus in primary health care units in Brazil
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Goes, Isabella Carolina Rodrigues dos Santos

; Romero, Letícia Calixto

; Turra, Ana Julia

; Gotardi, Maria Angélica

; Rodrigues, Talyssa Fernandes Silva de Oliveira

; Santos, Lalessa de Oliveira

; Dores, Jéssica Cristina das

; Nascimento, Michel Ulloffo do

; Cavalleri, Andressa Côrtes

; Pinheiro-Hubinger, Luiza

; Eller, Lizziane Kretli Winkelstroter

; Pereira, Valéria Cataneli

Revista do Instituto de Medicina Tropical de São Paulo

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2021, Volume 63 elocation e14

Abstract: En | Text: En | PDF: En

ABSTRACT Nasal carriage of Staphylococcus aureus by healthcare workers is of great clinical importance as it facilitates the contamination of medical devices and cross-transmission. However, studies regarding the epidemiology and dissemination of S. aureus and Methicillin-resistant S. aureus (MRSA) within the Primary Health Care in Brazil are scarce. The current study aimed to detect and characterize S. aureus and MRSA strains from the nasal cavities of 63 healthcare working in primary health care units in order to determine the prevalence of S. aureus and MRSA, biofilm formation and resistance profile of these isolates. PCR reactions were performed for detecting mecA, icaA and icaD genes. The phenotypic antimicrobial susceptibility was assessed by the disk diffusion method and biofilm formation by the Congo Red Agar (CRA) method. The MRSA isolates were typed for the Staphylococcal Cassette Chromosome mec (SCCmec). The prevalence of nasal carriage of S. aureus was 74.6%, of which 72.3% were MRSA carrying SCCmec type I (24.4%), III (34.1%), IV (36.6%). Two (4.9%) isolates presented a non-typeable cassette by the performed technique. The antimicrobial susceptibility evaluation evidenced penicillin resistance in 66.1% of S. aureus, erythromycin resistance in 49.2%, while 37.3% were resistant to oxacillin, 28.8% to cefoxitin, 5.1% to levofloxacin and 5.1% to clindamycin. All isolates were biofilm producers and 96.6% of the strains contained the ica biofilm-forming genes (icaA and/or icaD). We have demonstrated a high prevalence of S. aureus and MRSA carriage among health care working in Primary Health Care units, the presence of SCCmec types I, III and IV, in addition to their high ability to form biofilm, factors that possibly contribute to the dissemination and persistence of these pathogens within the primary care services. These observations highlight the importance of broadening the perspective of Health Care-Associated Infections prevention, including all health care levels, which are currently little explored. In addition, the dynamics and resistance mechanisms of S. aureus transmission still need to be further clarified to enable the implementation of more effective prevention measures.

https://doi.org/10.1590/s1678-9946202163014 661 downloads

10.

Complete denture hygiene solutions: antibiofilm activity and effects on physical and mechanical properties of acrylic resin
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ROCHA, Millena Mangueira

; CARVALHO, Adrianne Moura

; COIMBRA, Flávia Cristina Targa

; ARRUDA, Carolina Noronha Ferraz de

; OLIVEIRA, Viviane de Cássia

; MACEDO, Ana Paula

; SILVA-LOVATO, Cláudia Helena

; PAGNANO, Valéria Oliveira

; PARANHOS, Helena de Freitas Oliveira

Journal of Applied Oral Science

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2021, Volume 29 elocation e20200948

Abstract: En | Text: En | PDF: En

Abstract Appropriated denture hygiene is a predictive factor for longevity of rehabilitation treatment and maintenance of the oral mucosal health. Although, disinfectant solutions are commonly used as denture cleansers, the impact of these solutions on acrylic resin-based dentures remain unclear. Objective To evaluate, in vitro, the antibiofilm activity of complete denture hygiene solutions and their effects on physical and mechanical properties of acrylic resin. Methodology For antibiofilm activity measurement acrylic resin specimens were contaminated with Candida albicans, Candida glabrata and Streptococcus mutans. After biofilm growth, the specimens were assigned to the hygiene solutions: Distilled water (Control); 0.2% Sodium hypochlorite (SH); Efferdent Power Clean Crystals (EPC) and 6.25% Ricinus communis (RC). The viability of microorganisms was evaluated by agar plate counts. In parallel, physical, and mechanical properties of the acrylic resin were evaluated after simulating a 5-year period of daily immersion in the previously mentioned solutions. The changes in surface roughness, color, microhardness, flexural strength, impact strength, sorption and solubility were evaluated. Data were compared by ANOVA followed by the Tukey test or Kruskal-Wallis followed by the Dunn test depending on the distribution (α=0.05). Results Regarding antibiofilm action, SH eliminated all microorganisms while EPC and RC exhibited moderate action against S. mutans (p=0.001) and C. glabrata (p<0.001), respectively. Relative to effects on the physical and mechanical properties of the acrylic resin, RC led to higher values of color change (p=0.030), hardness (p<0.001), surface roughness (p=0.006) and flexural strength (p<0.001). Moreover, RC induced the highest values of changes in solubility (p<0.001). EPC promoted greater changes in surface morphology, whereas immersion in SH retained the initial appearance of the acrylic resin surface. All hygiene solutions reduced the impact strength (p<0.05). Conclusion SH presented the most effective antibiofilm activity. In addition, changes on properties were observed after immersion in RC, which were considered within acceptable limits.

https://doi.org/10.1590/1678-7757-2020-0948 151 downloads

11.

COVID-19: The question of genetic diversity and therapeutic intervention approaches
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Figueiredo, David Livingstone Alves

; Ximenez, João Paulo Bianchi

; Seiva, Fábio Rodrigues Ferreira

; Panis, Carolina

; Bezerra, Rafael dos Santos

; Ferrasa, Adriano

; Cecchini, Alessandra Lourenço

; Medeiros, Alexandra Ivo de

; Almeida, Ana Marisa Fusco

; Ramão, Anelisa

; Boldt, Angelica Beate Winter

; Moya, Carla Fredrichsen

; Chin, Chung Man

; Paula, Daniel de

; Rech, Daniel

; Gradia, Daniela Fiori

; Malheiros, Danielle

; Venturini, Danielle

; Tavares, Eliandro Reis

; Carraro, Emerson

; Ribeiro, Enilze Maria de Souza Fonseca

; Pereira, Evani Marques

; Tuon, Felipe Francisco

; Follador, Franciele Aní Caovilla

; Fernandes, Glaura Scantamburlo Alves

; Volpato, Hélito

; Cólus, Ilce Mara de Syllos

; Oliveira, Jaqueline Carvalho de

; Rodrigues, Jean Henrique da Silva

; Santos, Jean Leandro dos

; Visentainer, Jeane Eliete Laguila

; Brandi, Juliana Cristina

; Serpeloni, Juliana Mara

; Bonini, Juliana Sartori

; Oliveira, Karen Brajão de

; Fiorentin, Karine

; Lucio, Léia Carolina

; Faccin-Galhardi, Ligia Carla

; Ferreto, Lirane Elize Defante

; Lioni, Lucy Megumi Yamauchi

; Consolaro, Marcia Edilaine Lopes

; Vicari, Marcelo Ricardo

; Arbex, Marcos Abdo

; Pileggi, Marcos

; Watanabe, Maria Angelica Ehara

; Costa, Maria Antônia Ramos

; Giannini, Maria José S. Mendes

; Amarante, Marla Karine

; Khalil, Najeh Maissar

; Lima Neto, Quirino Alves de

; Herai, Roberto H.

; Guembarovski, Roberta Losi

; Shinsato, Rogério N.

; Mainardes, Rubiana Mara

; Giuliatti, Silvana

; Yamada-Ogatta, Sueli Fumie

; Gerber, Viviane Knuppel de Quadros

; Pavanelli, Wander Rogério

; Silva, Weber Claudio da

; Petzl-Erler, Maria Luiza

; Valente, Valeria

; Soares, Christiane Pienna

; Cavalli, Luciane Regina

; Silva Jr, Wilson Araujo

Genetics and Molecular Biology

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2021, Volume 44 N. 1 elocation e20200452

Abstract: En | Text: En | PDF: En

Abstract Coronavirus disease 2019 (COVID-19), caused by the Severe Acute Respiratory Syndrome Coronavirus type 2 (SARS-CoV-2), is the largest pandemic in modern history with very high infection rates and considerable mortality. The disease, which emerged in China’s Wuhan province, had its first reported case on December 29, 2019, and spread rapidly worldwide. On March 11, 2020, the World Health Organization (WHO) declared the COVID-19 outbreak a pandemic and global health emergency. Since the outbreak, efforts to develop COVID-19 vaccines, engineer new drugs, and evaluate existing ones for drug repurposing have been intensively undertaken to find ways to control this pandemic. COVID-19 therapeutic strategies aim to impair molecular pathways involved in the virus entrance and replication or interfere in the patients’ overreaction and immunopathology. Moreover, nanotechnology could be an approach to boost the activity of new drugs. Several COVID-19 vaccine candidates have received emergency-use or full authorization in one or more countries, and others are being developed and tested. This review assesses the different strategies currently proposed to control COVID-19 and the issues or limitations imposed on some approaches by the human and viral genetic variability.

https://doi.org/10.1590/1678-4685-gmb-2020-0452

12.

Synthesis, Characterization and in vitro, in vivo and in silico Anti-Inflammatory Studies of the Novel Hybrid Based on Ibuprofen and 3-Hydroxy-Copalic Acid Isolated from Copaiba Oil (Copaifera multijuga)
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Souza, Fábio C. de

; Brito, Larissa F.

; Silva, Marcos T.

; Sugimoto, Michelle A.

; Pinto, Ana Cristina S.

; Almeida, Patrícia D. O. de

; Souza, Rodrigo O. S.

; Costa, Renyer A.

; Guilhon-Simplicio, Fernanda

; Wanderley, Almir G.

; Oliveira, Kelson M. T. de

; Sousa, Lirlândia P.

; Veiga-Junior, Valdir F. da

; Lima, Emerson S.

Journal of the Brazilian Chemical Society

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jul 2020, Volume 31 N. 7 Pages 1335 - 1344

Abstract: En | Text: En | PDF: En

A novel anti-inflammatory hybrid 3-ibuprofenyl-copalic acid (3-IbuCA) was synthesized from 3-hydroxy-copalic acid isolated from Amazonian copaiba oil (Copaifera multijuga Hayne), and the anti-inflammatory ibuprofen. After full characterization, several assays to verify its anti-inflammatory effects were performed in vitro, in vivo and in silico (molecular docking). Induced fit docking was performed to observe the interactions with the enzymes cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2). In vitro tests of cytotoxicity and tumor necrosis factor (TNF)-α inhibition, and in vivo tests of pleurisy, protein expression and gastrocytotoxicity were performed. Molecular docking studies with COX-1 and 2 showed binding free energies (ΔG) of -2.2 and -7.8 kcal mol-1, respectively, while for mofezolac and indomethacin, the binding free energies ΔG presented values of -8.5 and -10.1 kcal mol-1, which makes 3-IbuCA selective for COX-2 inhibition. This hybrid showed no toxicity against human macrophage at concentrations up to 2 µM, and inhibited TNF-α production in lipopolysaccharide (LPS)-stimulated macrophages. In the pleurisy assays, 3-IbuCA reduced the total leukocytes and mononuclear cells, which was followed by reduction of p-IKBα (phosphorylated nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha) protein expression. Compared with ibuprofen alone, the hybrid caused less gastric damage. Thus, the docking, together with in vitro and in vivo studies suggest that this novel hybrid has potential as a new anti-inflammatory agent.

https://doi.org/10.21577/0103-5053.20190266 429 downloads

13.

Guideline of the Brazilian Society of Cardiology on Telemedicine in Cardiology - 2019
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Lopes, Marcelo Antônio Cartaxo Queiroga

; Oliveira, Gláucia Maria Moraes de

; Ribeiro, Antonio Luiz Pinho

; Pinto, Fausto J.

; Rey, Helena Cramer Veiga

; Zimerman, Leandro Ioschpe

; Rochitte, Carlos Eduardo

; Bacal, Fernando

; Polanczyk, Carisi Anne

; Halperin, Cidio

; Araújo, Edson Correia

; Mesquita, Evandro Tinoco

; Arruda, José Airton

; Rohde, Luis Eduardo Paim

; Grinberg, Max

; Moretti, Miguel

; Caramori, Paulo Ricardo Avancini

; Botelho, Roberto Vieira

; Brandão, Andréa Araújo

; Hajjar, Ludhmila Abrahão

; Santos, Alexandre Fonseca

; Colafranceschi, Alexandre Siciliano

; Etges, Ana Paula Beck da Silva

; Marino, Bárbara Campos Abreu

; Zanotto, Bruna Stella

; Nascimento, Bruno Ramos

; Medeiros, Cesar Rocha

; Santos, Daniel Vitor de Vasconcelos

; Cook, Daniela Matos Arrowsmith

; Antoniolli, Eduardo

; Souza Filho, Erito Marques de

; Fernandes, Fábio

; Gandour, Fabio

; Fernandez, Francisco

; Souza, Germano Emilio Conceição

; Weigert, Guilherme de Souza

; Castro, Iran

; Cade, Jamil Ribeiro

; Figueiredo Neto, José Albuquerque de

; Fernandes, Juliano de Lara

; Hadlich, Marcelo Souza

; Oliveira, Marco Antonio Praça

; Alkmim, Maria Beatriz

; Paixão, Maria Cristina da

; Prudente, Maurício Lopes

; Aguiar Netto, Miguel A. S.

; Marcolino, Milena Soriano

; Oliveira, Monica Amorim de

; Simonelli, Osvaldo

; Lemos Neto, Pedro A.

; Rosa, Priscila Raupp da

; Figueira, Renato Minelli

; Cury, Roberto Caldeira

; Almeida, Rodrigo Coelho

; Lima, Sandra Regina Franco

; Barberato, Silvio Henrique

; Constancio, Thiago Inocêncio

; Rezende, Wladimir Fernandes de

Arquivos Brasileiros de Cardiologia

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nov 2019, Volume 113 N. 5 Pages 1006 - 1056

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.5935/abc.20190205 5274 downloads

14.

Concomitance of Aids and cysticercosis in autopsies
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Cavellani, Camila L.

; Favarato, Grace Kelly N. A.

; Silva, Aline Cristina S.

; Oliveira, Lívia F.

; Faleiros, Ana Carolina G.

; Corrêa, Rosana Rosa M.

; Lino-Júnior, Ruy S.

; Teixeira, Vicente de Paula A.

Jornal Brasileiro de Patologia e Medicina Laboratorial

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jun 2019, Volume 55 N. 3 Pages 305 - 314

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO O objetivo do presente trabalho foi relatar a concomitância entre cisticercose e síndrome da imunodeficiência adquirida (Aids) em pacientes autopsiados em um hospital escola de Minas Gerais, Brasil. É possível associar cisticercose e pacientes com Aids, mas eles podem ou não apresentar os sintomas, dependendo do seu estado imunológico. Por isso, é necessário que a cisticercose entre no rol de infecções oportunistas e que mais estudos sejam feitos para melhor compreensão dessa coinfecção.

ABSTRACT The objective of the present study was to report the concomitance of cysticercosis and acquired immunodeficiency syndrome (Aids) in autopsied patients at a school hospital in Minas Gerais State, Brazil. Cysticercosis can be seen in patients with Aids, who may or may not present symptoms according to their immunological state. Therefore, it is necessary that cysticercosis be included in the list of opportunistic infections, and that more studies be carried out for a better understanding of this coinfection.

RESUMEN El objetivo del presente trabajo es reportar la concomitancia entre cisticercosis y el síndrome de inmunodeficiencia adquirida (Sida) en pacientes sometidos a autopsia en un hospital universitario de Minas Gerais, Brasil. Es posible asociar cisticercosis y pacientes con Sida, pero ellos pueden presentar síntomas o no, según su situación inmunitaria. Por eso, se recomienda incluir la cisticercosis en la lista de infecciones oportunistas y realizar más estudios para mejorar la comprensión de esa coinfección.

https://doi.org/10.5935/1676-2444.20190025 1039 downloads

15.

Is arbuscular mycorrhizal fungal species community affected by cotton growth management systems in the Brazilian Cerrado?
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NUNES, HELIAB B.

; GOTO, BRUNO T.

; COIMBRA, JOÃO LUIZ

; OLIVEIRA, JAMILE S.

; TAVARES, DÉRICA G.

; ROCHA, MARCELO S.

; SILVA, FABIANE L.

; SOARES, ANA CRISTINA F.

Anais da Academia Brasileira de Ciências

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2019, Volume 91 N. 4 elocation e20180695

Abstract: En | Text: En | PDF: En

Abstract: Conventional cotton production in western Bahia, Brazil, involves intensive use of agricultural inputs and mechanization, which may affect arbuscular mycorrhizal fungi (AMF). This work aimed at studying the impact of conventional and organic cotton production in the AMF of western Bahia. Soil samples were obtained from conventional white cotton and colored cotton organic production systems as well as from native Cerrado areas, close to the white cotton fields, and from the subcaducifolia vegetation, close to the organic colored cotton farms. The most frequent species in the conventional farming areas belonged to the genera Acaulospora (10 spp.); Glomus (8 spp.); Dentiscutata (3 spp.); Ambispora, Pacispora and Scutellospora (2 spp. each), as well as Claroideoglomus etunicatum, Diversispora sp., Entrophospora infrequens, Gigaspora sp., Orbispora pernambucana, Paradentiscutata maritima, and Paraglomus occultum. Eighteen species were found in the organic farming areas, with the predominance of Glomus (5 spp.) and Acaulospora (5 spp.), and with Claroideoglomus, Dentiscutata, Gigaspora, Corymbiglomus, Orbispora, Paraglomus, Scutellospora, and Simiglomus (1 spp. each). Paraglomus bolivianum was first reported in Cerrado. In the native vegetation, nine species were found, with the predominance of Glomus and Acaulospora. The highest number of AMF species was found in the organic farming areas, which deserves further investigation.

https://doi.org/10.1590/0001-3765201920180695 657 downloads

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