ABSTRACT Objective: To create and validate a model for predicting septic or hypovolemic shock from easily obtainable variables collected from patients at admission to an intensive care unit. Methods: A predictive modeling study with concurrent cohort data was conducted in a hospital in the interior of northeastern Brazil. Patients aged 18 years or older who were not using vasoactive drugs on the day of admission and were hospitalized from November 2020 to July 2021 were included. The Decision Tree, Random Forest, AdaBoost, Gradient Boosting and XGBoost classification algorithms were tested for use in building the model. The validation method used was k-fold cross validation. The evaluation metrics used were recall, precision and area under the Receiver Operating Characteristic curve. Results: A total of 720 patients were used to create and validate the model. The models showed high predictive capacity with areas under the Receiver Operating Characteristic curve of 0.979; 0.999; 0.980; 0.998 and 1.00 for the Decision Tree, Random Forest, AdaBoost, Gradient Boosting and XGBoost algorithms, respectively. Conclusion: The predictive model created and validated showed a high ability to predict septic and hypovolemic shock from the time of admission of patients to the intensive care unit.

RESUMO Objetivo: Criar e validar um modelo de predição de choque séptico ou hipovolêmico a partir de variáveis de fácil obtenção coletadas na admissão de pacientes internados em uma unidade de terapia intensiva. Métodos: Estudo de modelagem preditiva com dados de coorte concorrente realizada em um hospital do interior do nordeste brasileiro. Foram incluídos pacientes com 18 anos ou mais sem uso de droga vasoativa no dia da admissão e que foram internados entre novembro de 2020 e julho de 2021. Foram testados os algoritmos de classificação do tipo Decision Tree, Random Forest, AdaBoost, Gradient Boosting e XGBoost para a construção do modelo. O método de validação utilizado foi o k-fold cross validation. As métricas de avaliação utilizadas foram recall, precisão e área sob a curva Receiver Operating Characteristic. Resultados: Foram utilizados 720 pacientes para criação e validação do modelo. Os modelos apresentaram alta capacidade preditiva com área sob a curva Receiver Operating Characteristic de 0,979; 0,999; 0,980; 0,998 e 1,00 para os algoritmos de Decision Tree, Random Forest, AdaBoost, Gradient Boosting e XGBoost, respectivamente. Conclusão: O modelo preditivo criado e validado apresentou elevada capacidade de predição do choque séptico e hipovolêmico desde o momento da admissão de pacientes na unidade de terapia intensiva.

Abstract Introduction: patients with COVID-19 undergo changes in leukocyte count, respiratory disorders, and an increase in inflammatory substances. To improve the inflammatory condition, some nutrients can be used, including arginine, omega-3 fatty acids and nucleotides. This study aims to evaluate how oral immunonutrient supplements affects serum C-reactive protein (CRP) levels and lymphocyte count in patients with COVID-19. Methods: in this double-blind clinical trial, we randomized 43 adult patients with COVID-19 to receive a standard high-protein normocaloric supplement (control) or an immunonutrient-enriched supplement (experiment) for 7 days. The primary outcome was to evaluate changes in total lymphocyte count and serum level of CRP. The assessment of risk and nutritional status of these patients was also performed. Results: forty-three patients with mean age of 41.5 (± 1.8) years were followed up, 39.5 % of them women. The mean body mass index was 27.6 (± 0.8) kg/m² and 58.1 % had low nutritional risk. In the experiment group, there was a CRP reduction of 23.6 (± 7.5) mg/L, while in the control branch the decrease was 14.8 (± 12.1) mg/L (p = 0.002). There was an increase in lymphocytes in the experiment group (+367.5 ± 401.8 cells/mm³) and a reduction in the control group (-282.8 ± 327.8 cells/mm³), although there was no statistical significance (p = 0.369). Relative risk (RR) of treatment in reducing CRP by 30 % or more was 4.45 (p < 0.001; 95 % CI, 1.79-11.07). RR in increasing lymphocyte count by 30 % or more was 1.28 (p = 0.327; 95 % CI, 0.67-2.45). Conclusion: we conclude that immunonutrient supplements seem to reduce CRP levels more than standard high-protein normocaloric supplements.

Resumen Introducción: los pacientes con COVID-19 sufren cambios en el recuento de leucocitos, trastornos respiratorios y aumento de sustancias inflamatorias. Para mejorar la condición inflamatoria se pueden usar algunos nutrientes, como la arginina, los ácidos grasos omega-3 y los nucleótidos. Este estudio tiene como objetivo evaluar cómo los suplementos de inmunonutrientes orales afectan a los niveles séricos de proteína C-reactiva (PCR) y al recuento de linfocitos en pacientes con COVID-19. Métodos: en este ensayo clínico doble ciego, aleatorizamos a 43 pacientes adultos con COVID-19 para recibir un suplemento normocalórico estándar alto en proteínas (control) o un suplemento enriquecido con inmunonutrientes (experimento) durante 7 días. El resultado primario fue evaluar los cambios en el recuento total de linfocitos y el nivel sérico de PCR. También se realizó la evaluación del riesgo y el estado nutricional de estos pacientes. Resultados: cuarenta y tres pacientes con edad media de 41,5 (± 1,8) años fueron seguidos, el 39,5 % de ellos mujeres. El índice de masa corporal medio fue de 27,6 (± 0,8) kg/m² y el 58,1 % tenían bajo riesgo nutricional. En el grupo experimental hubo una reducción de la PCR de 23,6 (± 7,5) mg/L, mientras que en la rama de control la disminución fue de 14,8 (± 12,1) mg/L (p = 0,002). Hubo un aumento de linfocitos en el grupo experimental (+367,5 ± 401,8 células/mm³) y una reducción en el grupo de control (-282,8 ± 327,8 células/mm³), aunque no hubo significación estadística (p = 0,369). El riesgo relativo (RR) del tratamiento para reducir la PCR en un 30 % o más fue de 4,45 (p < 0,001; IC 95 %: 1,79-11,07). El RR en el aumento del recuento de linfocitos en un 30 % o más fue de 1,28 (p = 0,327; IC 95 %: 0,67-2,45). Conclusión: se concluye que los suplementos de inmunonutrientes parecen reducir los niveles de PCR más que los suplementos normocalóricos estándar altos en proteína.

Abstract Background Endogenous phospholipases A2 (PLA2) play a fundamental role in inflammation, neurodegenerative diseases, apoptosis and cellular senescence. Neurotoxins with PLA2 activity are found in snake venoms from the Elapidae and Viperidae families. The mechanism of action of these neurotoxins have been studied using hippocampal and cerebellar neuronal cultures showing [Ca2+]i increase, mitochondrial depolarization and cell death. Astrocytes are rarely used as a model, despite being modulators at the synapses and responsible for homeostasis and defense in the central nervous system. Preserving the cell division ability, they can be utilized to study the cell proliferation process. In the present work cultured astrocytes and glioblastoma cells were employed to characterize the action of β-micrustoxin (previously named Mlx-9), a PLA2 isolated from Micrurus lemniscatus snake venom. The β-micrustoxin structure was determined and the cell proliferation, cell cycle phases and the regulatory proteins p53, p21 and p27 were investigated. Methods β-micrustoxin was characterized biochemically by a proteomic approach. Astrocytes were obtained by dissociation of pineal glands from Wistar rats; glioblastoma tumor cells were purchased from ATCC and Sigma and cultured in DMEM medium. Cell viability was evaluated by MTT assay; cell proliferation and cell cycle phases were analyzed by flow cytometry; p53, p21 and p27 proteins were studied by western blotting and immunocytochemistry. Results Proteomic analysis revealed fragments on β-micrustoxin that aligned with a PLA2 from Micrurus lemniscatus lemniscatus previously identified as transcript ID DN112835_C3_g9_i1/m.9019. β-micrustoxin impaired the viability of astrocytes and glioblastoma tumor cells. There was a reduction in cell proliferation, an increase in G2/M phase and activation of p53, p21 and p27 proteins in astrocytes. Conclusion These findings indicate that β-micrustoxin from Micrurus lemniscatus venom could inhibit cell proliferation through p53, p21 and p27 activation thus imposing cell cycle arrest at the checkpoint G2/M.

ABSTRACT The Amazon region is one of the most pristine continental areas whose concentrations of atmospheric trace gases and aerosol particles are very low, mainly in the wet season. This study provides observational results of aerosol optical and radiative characteristics in situ as well as atmospheric columnar at a pristine forest in Central Amazonia. Spectral variation of the aerosol properties (aerosol optical depth [AOD], single scattering albedo [SSA], and asymmetry parameter [AP]) was evaluated using the AErosol RObotic NETwork (AERONET) data. The SSA values under natural atmospheric column conditions (AERONET) were compared to the SSA values calculated with in situ measurements. The values of shortwave aerosol radiative forcing (SWARF) on top of the atmosphere (TOA) and the surface (SUR) were estimated using the SBDART model and were validated with the AERONET values with regression analysis. SWARF had a high correlation to TOA (0.97) and SUR (0.92), including dry and wet seasons. Monthly, seasonal and annual mean values of SWARFTOA and SWARFSUR were negative while SWARFATM values were positive. SWARFTOA was -9.18 ± 2.80 W m-2 and SWARFSUR was -20.77 ± 5.04 W m-2 in the dry season, inducing a heating rate (HR) of 0.37 ± 0.13 K.day-1. This study showed that, for a long series of measurements, the effects caused by aerosols on the radiative flux in the pristine forest of Central Amazonia were of the order of SWARFTOA of -3.66 ± 1.59 W m-2 and SWARFSUR of -11.86 ± 2.35 W m-2 during the wet season.

RESUMEN La región del Amazonas es una de las áreas continentales más impolutas, cuyas concentraciones de gases traza atmosféricos y partículas de aerosol son muy bajas, sobre todo en la estación húmeda. El presente estudio proporciona resultados observacionales in situ tanto de las características ópticas y radiativas como de la columna atmosférica de los aerosoles en un bosque virgen de la Amazonia Central. Se evaluó la variación espectral de las propiedades de los aerosoles (profundidad óptica de aerosoles [AOD, por sus siglas en inglés], albedo de dispersión simple [SSA] y parámetro de asimetría [AP]) utilizando para ello datos de la Red Robótica de Aerosoles (AERONET). Los valores de SSA en condiciones naturales de la columna atmosférica (obtenidos de AERONET) se compararon con valores de SSA obtenidos de mediciones in situ. Se estimaron los valores del forzamiento radiativo de onda corta (SWARF) en el techo de la atmósfera (TOA) y en la superficie (SUR) con datos del modelo SBDART y se validaron con valores de AERONET utilizando análisis de regresión. Los valores de SWARF tuvieron una alta correlación con los de TOA (0.97) y SUR (0.92), incluyendo las temporadas húmeda y seca. Los valores promedio mensuales, estacionales y anuales de SWARFTOA y SWARFSUR fueron negativos, en tanto que los de SWARFATM fueron positivos. Los valores de SWARFTOA fueron de -9.18 ± 2.80 W m-2 y los de SWARFSUR de -20.77 ± 5.04 W m-2 en la estación seca, induciendo una tasa de calentamiento (HR) de 0.37 ± 0.13 K día-1. El presente estudio muestra que, para series largas de medición, los efectos ocasionados por los aerosoles en el flujo radiativo en un bosque virgen de la Amazonia Central fueron del orden de -3.66 ± 1.59 W m-2 para SWARFTOA y de -11.86 ± 2.35 W m-2 para SWARFSUR durante la temporada húmeda.

Abstract Objective: To evaluate the effectiveness of chewing gums containing CPP-ACP in remineralisation of demineralised enamel around orthodontic brackets. Material and Methods: Enamel blocks from 120 bovine incisors were used. The blocks were flattened and polished, followed by the development of incipient subsurface caries. The blocks have been subjected to a baseline microhardness analysis. Orthodontic brackets were bonded to the exposed area of the blocks, reserving an area for hardness analysis. An in situ crossover (three-way crossover phases of 21 days with an interval of one week between them), controlled, randomised and blind experimental model was used, with the participation of 12 volunteers divided into groups: G1 – negative control group, without chewing gum; G2 – conventional sugar-free chewing gum, without CPP-ACP (placebo); and G3 – sugar-free chewing gum with CPP-ACP. The following parameters were analysed: superficial linear (Ra), volumetric roughness (Sa), and superficial structural loss, which was indicated by Gap measurement (difference between the healthy and treated area). Statistical tests used were the Friedman, Wilcoxon and Shapiro-Wilk, Kruskal Wallis' nonparametric tests and ANOVA. Results: There was a significant reduction in enamel microhardness after demineralisation in all the groups (p<0.05). This deleterious effect was attenuated in the three groups after the final evaluation, although there were no statistical differences (p<0.05); however, the final values did not return to the baseline values (p<0.05). Conclusion: Chewing gum containing CPP-ACP did not promote in situ remineralisation of demineralised enamel around orthodontic brackets.

Abstract The use of unconventional edible plants in human food has grown more widespread in recent years, driven mainly by gourmet dishes. Evidence of the food properties of these species is still scarce. Aiming to obtain more in-depth knowledge of dahlia plants, this study aimed to evaluate the nutritional, bioactive, and antinutritional properties of the edible parts of purple dahlia (tuberous root and flower) to elucidate their value and safety as foods. The percent composition, caloric value, pH, Total Soluble Solids (TSS), Total Titratable Acidity (TTA), ratio of TSS to TTA, anthocyanins, carotenoids, vitamin C, total phenolics, tannins, nitrates, and minerals of the roots and flowers were analyzed. The inulin content in the root and the color of the flower were also evaluated. The results were reported as mean and standard deviation, and exploratory factor analysis was performed. Both parts of the dahlia had constituents that contribute to a good diet at concentrations like those in conventional vegetables, whereas the antinutritional components were also compatible with those of commonly consumed foods, with acceptable dietary values. Thus, it is evident that purple dahlia flowers and roots have potential for use in human food and can be considered good options to improve and diversify a healthy diet.

Resumo A utilização de plantas alimentícias não convencionais na alimentação humana tem crescido nos últimos anos, impulsionada principalmente pelos pratos gourmet. Entretanto, estudos comprovando as propriedades alimentícias destas espécies ainda são incipientes. Sendo assim, objetivou-se com este trabalho avaliar as propriedades nutricionais, bioativas e antinutricionais das partes comestíveis da dália roxa (raiz tuberosa e flor), para elucidar seu potencial e a segurança no seu consumo. Foram, dessa forma, analisados: composição centesimal, valor calórico, pH, sólidos solúveis totais, acidez total titulável, ratio, antocianinas, carotenoides, vitamina C, fenólicos totais, taninos, nitratos e minerais para a raiz e a flor, além da avaliação do teor de inulina na raiz. Os resultados foram analisados pelas médias e desvio padrão, e foi realizada a análise fatorial exploratória. Ambas as partes avaliadas possuem constituintes requeridos para uma boa alimentação em concentrações semelhantes às das hortaliças convencionais, enquanto os componentes antinutricionais também são compatíveis aos de alimentos comumente consumidos, com valores aceitáveis na alimentação. Desse modo, é evidenciado que flor e raiz de dália roxa apresentam potencial para uso na alimentação humana, podendo ser consideradas como boas opções para incrementar e diversificar uma dieta saudável.

RESUMO O objetivo deste trabalho é, a partir dos preceitos da decolonialidade (QUIJANO, 2005; WALSH, 2009), analisar as listas de leituras obrigatórias de vestibulares, investigando: os efeitos retroativos dessas listas nas práticas de letramentos literários escolares; a diversidade social, étnica, sexual e de gênero que elas promovem; como movimentam o cânone literário escolar; e como contribuem (ou podem contribuir) para uma maior democratização da/na educação literária. Para isso, selecionamos listas dos últimos vestibulares de sete universidades públicas, buscando contemplar instituições de cada região do Brasil. Tomamos as listas de leituras como importante ferramenta de legitimação de autores e obras literárias para o contexto escolar. A análise proposta é qualitativo-interpretativista (MOITA-LOPES, 1994; 2006) e compreende que todas as práticas de letramentos literários são ideológicas (STREET, 2014), o que pressupõe tanto uma investigação em diálogo com os contextos sociais e com os diversos atores/agentes em torno das práticas de letramentos analisadas, quanto uma interpretação que não isole os dados da pesquisa das relações de poder e disputas discursivas exercidas em torno deles. A partir da constatação de que a inclusão promovida por essas listas de obras literárias se limita apenas a determinadas diversidades, procuramos analisar, especialmente, a ausência de autores e obras LGBTQIA+, a fim de compreender as razões dessa ausência e demonstrar o potencial educativo que obras e autores desses grupos sociais poderiam ter na construção de práticas de letramentos literários escolares de reexistência (SOUZA, 2011; WALSH, 2009) para uma educação transgressora (HOOKS, 2017) e democrática.

ABSTRACT This paper, based on the precepts of decoloniality (QUIJANO, 2005; WALSH, 2009), aims to analyze the required readings lists for college entrance examinations (vestibular) by investigating: the retroactive effects of these lists on school literary literacy practices; the social, ethnic, gender, and sexual diversity they promote; how they move the school literary canon; and how they contribute (or can contribute) to a greater democratization of/in literary education. To this end, we select lists from the latest vestibular examinations of seven public universities, seeking to include institutions from all regions of Brazil. We consider reading lists to be an important tool for legitimizing authors and literary works in the school context. The proposed analysis is qualitative-interpretative (MOITA-LOPES, 1994; 2006) and understands that all literary literacy practices are ideological (STREET, 2014), which presupposes not only an investigation in dialogue with social contexts and with the various actors/agents around the literacy practices analyzed, but also an interpretation that does not isolate the research data from the power relations and discursive disputes exercised around them. From the observation that the inclusion promoted by these lists of literary works is limited only to certain diversities, we seek to analyze, especially, the absence of LGBTQIA+ authors and works, in order to understand the reasons for this absence and to demonstrate the educational potential that works and authors from these social groups could have in the construction of school literary literacies practices of re-existence (SOUZA, 2011; WALSH, 2009) for a transgressive (HOOKS, 2017) and democratic education.

Resumo Este estudo teve por objetivo analisar como as situações envolvendo expressões numéricas são retratadas nos livros didáticos do sexto ano do Ensino Fundamental, a partir do enfoque da Teoria dos Campos Conceituais. Foram selecionadas sete coleções de livros didáticos utilizadas nas escolas de rede pública em um Município do interior do Rio Grande do Sul. Trata-se de um estudo documental com um corpus de análise de 47 atividades no formato de problemas. Foram analisados enunciados e resoluções de expressões numéricas, sua escrita e representação por diagrama, em problemas aditivos, multiplicativos e mistos. Os problemas abordam os campos conceituais de forma diversificada, com maior frequência de situações multiplicativas de proporção simples. Sugere-se que o trabalho com expressões numéricas seja realizado com o uso de diagramas e situações variadas, a fim de proporcionar a construção do conceito de operação e a compreensão das regras de prevalência e a necessidade da escrita dos sinais de associação pelos estudantes.

Abstract This study aimed to analyze how the situations involving numerical expressions are portrayed in the textbooks of the sixth year of Elementary School, according to the Theory of Conceptual Fields. Seven collections of textbooks used in public schools in a city in the countryside of Rio Grande do Sul were selected. This is a documental study with a corpus of analysis of 47 activities in the format of problems. We analyzed task instructions and resolutions of numerical expressions, their writing, and representation by diagram, in addition, multiplicative, and mixed problems. The problems approach the conceptual fields in a diversified way, with greater frequency of multiplicative situations of simple proportion. It is suggested that the work with numerical expressions be carried out with the use of diagrams and manifold situations, in order to provide the construction of the operation concept and the understanding of the rules of prevalence and the need for students to write association signs.

Resumo Fundamento: A mortalidade por doenças cardiovasculares (DCV) vem mostrando tendência à estabilização em alguns países, incluindo o Brasil e o estado do Rio de Janeiro, após décadas de queda. Não encontramos análises detalhadas dessa tendência para o estado do Rio de Janeiro. Objetivo: Analisar as tendências da mortalidade prematura e tardia por doenças do aparelho circulatório (DAC), doença isquêmica do coração (DIC) e doença cerebrovascular (DCBV) por sexo nas regiões de saúde do estado do Rio de Janeiro e capital (1996-2016). Métodos: Dados de óbitos e população foram obtidos no DATASUS/MS. Taxas foram compensadas por códigos mal definidos, corrigidos pelos códigos cardiovasculares mal definidos e ajustadas por sexo e idade pelo método direto. O Joinpoint Trend Analysis Software foi empregado para calcular a variação percentual anual (APC) e variação percentual anual média (AAPC). Foram consideradas para o estudo APC e AAPC significativamente diferentes de zero, calculadas por um teste de student com significância de 5%. Resultados: A mortalidade por DIC estabilizou ou até aumentou em pelo menos 50% das localidades analisadas (EAPC ≥0). Nas regiões Norte e Noroeste, nenhuma mudança foi observada. Para DCBV, apenas uma região apresentou estabilidade na mortalidade (EAPC próximo a 0). Para as outras regiões, a taxa continuou a diminuir (APC <0) até 2016. Conclusão: Esses resultados observados no Rio de Janeiro devem se repetir em várias regiões brasileiras e apontam para a necessidade de uma resposta na abordagem dos comportamentos no estilo de vida. Os médicos da atenção primária devem estar familiarizados com a tendência desfavorável da doença isquêmica do coração entre os adultos mais jovens e rastrear ativamente os fatores de risco para DCV, com atenção especial às mulheres.

Abstract Background: Cardiovascular disease (CVD) mortality, after several decades of decrease, has shown a tendency towards the stabilization in some countries, including Brazil and Rio de Janeiro state. This new tendency was not further analyzed by gender, age group and region of the Rio de Janeiro state. Objective: To analyze the trends of premature and late mortality from CVD, ischemic heart disease (IHD) and cerebrovascular disease (CBVD) by gender in the city of Rio de Janeiro (capital) and the health regions of Rio de Janeiro state (from 1996 to 2016. Methods: Data on deaths and the population were obtained from DATASUS/MS. The rates were compensated by ill-defined codes, corrected by Ill-Defined Cardiovascular codes and gender and age-adjusted by the direct method (reference population – population of the state of Rio de Janeiro - 2000 census). The Joinpoint Trend Analysis Software was employed. Results: IHD mortality stabilized or even increased for at least 50% of the analyzed areas (EAPC≥0). No change was observed. in the “North” and “Northwest” regions For CBVD, just one region showed stability regarding mortality (EAPC close to 0). For the other regions, the rate continued to decrease (APC<0) until 2016. Conclusion: These results observed in Rio de Janeiro are possibly appropriate to various Brazilian regions and demonstrate that a serious public health response is needed to address lifestyle behaviors. Primary care physicians should also be familiar with the unfavorable tendency in coronary heart disease among younger adults in recent years and actively screen for risk factors for cardiovascular disease, paying special attention to women.

Abstract The aim of this investigation was to evaluate the impact of multiple freezing and thawing cycles on the physicochemical properties of Nile tilapia fillets. For this purpose, 72 fresh Nile tilapia fillets were packed and stored in a freezer at -18 °C. The frozen samples were submitted to five freeze-thaw cycles; in each cycle, the freezer was switched off during 14 hours. The consecutive freeze-thaw cycles resulted in a fillet’s total weight loss of 9.48%, with a quadratic regression (P < 0.0001) for thaw loss, with a greater loss percentage in cycle 3 (2.68%). pH values differed between the cycles (P < 0.0001), being observed an increment in this parameter only from cycle 4. The lipid oxidation remained constant in cycles 1, 2, 4 and 5, however in cycle 3 the lowest value (P < 0.0002) was observed. The luminosity, and intensity of the red and yellow colours increased linearly (P < 0.0001) as the cycles increased. Thereby, the tilapia fillets’ quality were adversely affected by the freezing cycles. The unfavourable alterations included weight loss, lipid oxidation and elevated pH, as well as the increasingly augmentation of brightness and red and yellow colorations of fillets as the progressive cycles occurred.

Resumo Fundamento: Em muitas cidades no mundo, a taxa de mortalidade por câncer (CA) ultrapassou aquela por doenças do aparelho circulatório (DAC). Objetivos: Comparar as curvas de mortalidade por DAC e CA nas capitais mais populosas das cinco regiões brasileiras. Métodos: Dados de mortalidade por DAC e CA entre 2000 e 2015 foram coletadas no Sistema de Informações sobre Mortalidade das capitais mais populosas das cinco regiões do Brasil: Manaus, Salvador, Goiânia, São Paulo e Curitiba. Os dados foram categorizados por faixas etárias dos indivíduos em precoce (30-69 anos) e tardia (≥70 anos), e por gênero. Foram considerados os capítulos II e IX da Classificação Internacional de Doenças-10 para análise das causas de óbito. A tendência na estimativa de mudança percentual foi calculada pelo modelo de regressão Joinpoint 4.6.0.0. e a detecção das mudanças das taxas pelo teste de permutação Monte Carlo. Nível de significância estatística de 5%. Resultados: Observou-se queda consistente das mortalidades precoce e tardia por DAC, em ambos os gêneros, nas capitais estudadas, com exceção da mortalidade tardia em homens em Manaus. Houve tendência de queda das taxas de mortalidade por CA em São Paulo e Curitiba, e de aumento da taxa de mortalidade por CA em Goiânia. Em Salvador, houve queda na mortalidade precoce por CA em homens e mulheres e incremento na mortalidade tardia em ambos os gêneros. Conclusão: Houve queda progressiva e expressiva da taxa de mortalidade por DAC nas cinco capitais em oposição à manutenção ou discreta elevação da mortalidade por CA. Tais fenômenos concorrem para o cruzamento das curvas com predomínio da mortalidade por CA (já ocorrido ou casos novos).

Abstract Background: In many cities around the world, the mortality rate from cancer (CA) has exceeded that from disease of the circulatory system (DCS). Objectives: To compare the mortality curves from DCS and CA in the most populous capital cities of the five regions of Brazil. Methods: Data of mortality rates from DCS and CA between 2000 and 2015 were collected from the Mortality Information System of Manaus, Salvador, Goiania, Sao Paulo and Curitiba, and categorized by age range into early (30-69 years) and late (≥ 70 years), and by gender of the individuals. Chapters II and IX of the International Classification of Diseases-10 were used for the analysis of causes of deaths. The Joinpoint regression model was used to assess the tendency of the estimated annual percentage change of mortality rate, and the Monte Carlo permutation test was used to detect when changes occurred. Statistical significance was set at 5%. Results: There was a consistent decrease in early and late mortality from DCS in both genders in the cities studied, except for late mortality in men in Manaus. There was a tendency of decrease of mortality rates from CA in São Paulo and Curitiba, and of increase in the rates from CA in Goiania. In Salvador, there was a decrease in early mortality from CA in men and women and an increase in late mortality in both genders. Conclusion: There was a progressive and marked decrease in the mortality rate from DCS and a maintenance or slight increase in CA mortality in the five capital cities studied. These phenomena may lead to the intersection of the curves, with predominance of mortality from CA (old and new cases).

RESUMO Misturas asfálticas são os principais tipos de materiais que compõem as camadas de revestimentos asfálticos dos pavimentos brasileiros. Sabendo disso, este estudo teve por objetivo estudar misturas asfálticas, conhecidas como Concreto Asfáltico Estocável (CAE), que tem como diferencial a proposta de poder ser armazenada a granel ou ensacada e aplicada à frio. Foram realizadas as caracterizações físicas, volumétricas e mecânicas de três amostras de CAE de diferentes fabricantes com vistas a compreender sua composição e comportamento mecânico. Além disso, foram realizadas avaliações da granulometria utilizando as metodologias Bailey e Frações de Agregados Dominantes (FAD). Os resultados mostraram que as misturas de CAE não atenderam aos requisitos especificados em normas brasileiras e apresentaram resultados diferentes do esperado para um concreto asfáltico convencional, indicando que o mesmo não deve ser abordado como tal. Além disso, o CAE não atendeu aos parâmetros de trabalhabilidade e volumetria. Os resultados de viscosidade do ligante, do módulo dinâmico e do Flow Number indicaram que uma das amostras apresenta alta rigidez, fato que pode estar relacionado ao envelhecimento do ligante asfáltico e consequente enrijecimento da mistura. Por fim, verificou-se que o CAE apresenta envelhecimento durante a estocagem, o que não é adequado para misturas asfálticas. Logo, conclui-se que o mesmo, sem a adoção de critérios de controle de produção e aplicação, não é adequado para utilização como nova camada de revestimento, tendo seu uso limitado à serviços de manutenção como tapa-buraco.

ABSTRACT Asphalt mixtures are the main types of materials that make up the asphalt surface layers of Brazilian pavements. In this way, this study aimed to study asphalt mixtures, known as Stock Asphalt Concrete (CAE), which has as its differential the proposal to be stored in bulk or bagged and applied cold. The physical, volumetric and mechanical characterizations of three CAE samples from different manufacturers were performed to understand its composition and mechanical behavior. In addition, particle size evaluations were performed using Bailey and Dominant Aggregate Size Range (DASR) methodologies. The results showed that CAE mixtures did not meet the Brazilian standard requirements and presented different results than expected for a conventional asphalt concrete, indicating that it should not be approached as such. In addition, CAE did not meet the workability and volume parameters. The binder viscosity, dynamic modulus and Flow Number results indicated that one of the samples has high rigidity, which may be related to the aging of the asphalt binder and consequent stiffening of the mixture. Finally, it was found that CAE is aging during storage, which is not suitable for asphalt mixtures. Therefore, one concluded that CAE, without the adoption of production and application control criteria, cannot be used as a new surface course, and its application is limited only for maintenance works as blacktop.

Resumo: O objetivo deste trabalho foi avaliar a solubilidade dos micronutrientes cobre, níquel e zinco, extraídos quimicamente de agrominerais silicáticos, como fonte alternativa de potássio para as culturas de alface (Lactuca sativa) e arroz (Oryza sativa), bem como sua disponibilidade para as plantas. Avaliaram-se os teores de micronutrientes de cinco agrominerais (ultramáfica alcalina, biotita xisto, brecha vulcânica, flogopita xisto e subproduto de mineração de Chapada), após extração pelos métodos ácido cítrico, ácido clorídrico, ácido dietilenotriamino penta-acético, 3051A, citrato neutro de amônio e Mehlich 3. Os agrominerais, nas doses de 0 (controle), 200, 400 e 600 kg ha-1 de K, foram misturados em solo com cultivo de alface e arroz. Os agrominerais apresentaram baixa solubilidade de Ni, Cu e Zn, com menores teores em folhas de alface e grãos de arroz que o padrão solúvel correspondente na forma de sulfato. A disponibilidade de micronutrientes para as plantas aumentou com uso dos agrominerais, em comparação ao controle. Foram observadas similaridades na solubilidade e na disponibilidade de micronutrientes entre os diferentes grupos de agrominerais. A solubilidade e a disponibilidade de Cu, Ni e Zn dependem da solubilidade dos minerais que compõem os agrominerais.

Abstract: The objective of this work was to evaluate the solubility of the micronutrients copper, nickel, and zinc, chemically extracted from silicate agrominerals, as alternative sources of potassium for lettuce (Lactuca sativa) and rice (Oryza sativa) cultures, as well as their availability to the plants. The micronutrient contents of five agrominerals (alkaline ultramafic, biotite schist, volcanic breccia, phlogopite schist, and mining by-product from Chapada) were evaluated, after being extracted by the citric acid, hydrochloric acid, diethylenetriaminepentaacetic acid, 3051A, neutral ammonium citrate, and Mehlich-3 methods. The agrominerals, at the doses of 0 (control), 200, 400, and 600 kg ha-1 K, were mixed to soil where lettuce and rice were cultivated. The agrominerals presented a low solubility of Ni, Cu, and Zn, with lower contents in lettuce shoots and rice grains than the corresponding soluble standards in sulfate form. The availability of micronutrients to the plants increased with the use of agrominerals, in comparison with the control. Similarities were observed in micronutrient solubility and availability between the different groups of agrominerals. The solubility and availability of Cu, Ni, and Zn depend on the solubility of the minerals that compose the agrominerals.

ABSTRACT Considering that the beneficial effects of silicon on human health and it´s accumulation in plants are little explored, studies of mineral nutrition in non-conventional vegetables are extremely relevant. Thus, the objective of this work was to identify non-conventional vegetables responsive to silica fertilization and evaluate the effect of fertilization on the content and accumulation of Si and macronutrients in these plants. The experiment followed a 4 x 5 factorial design, with four species of non-conventional vegetables (Rumex acetosa L., Amaranthus retroflexus L., Sonchus oleraceus L. and Stachys byzantina) and five doses of Si (0, 25, 50, 100 and 150 mg dm-3). The relative chlorophyll index, the fresh and dry matter masses, the content and accumulation of Si and macronutrients were evaluated. The species A. retroflexus, S. oleraceus. and S. byzantina showed the highest contents and accumulation of Si. The application of doses above 100 mg dm-3 promoted the highest silicon content estimated for the species. The species A. retroflexus, R. acetosa and S. byzantina are non-silicon accumulators, whereas S. oleraceus is intermediate.

ABSTRACT Objective: Evaluate the inhibition of venoms from the snakes Bothrops alternatus and Bothrops moojeni by plant extracts from Jatropha curcas, Myrsine guianensis and Zanthoxylum monogynum in terms of coagulant, phospholipase and hemorrhagic activities. Methods: The plant extracts were obtained by lyophilization of leaves of Jatropha curca, Myrsine guianensis and Zanthoxylum monogynum. In vitro inhibition tests were performed with a positive control group (10 μg of crude venom), experimental groups receiving a mixture of the venom with each of the extracts at concentrations of 5 to 500 μg, and a negative control group (500 μg of extract). In vivo tests were performed with Balb/c mice (n = 4). The mice in the positive control group were injected only the venom (25 μg), the experimental groups received a mixture of the venom with each of the extracts at concentrations of 25 to 500 μg, and the negative control group received only extract (500 μg). Results: Jatropha curcas and Myrsine guianensis significantly inhibited phospholipase activity at a 1:0.5 (venom:extract) proportion. Coagulant and hemorrhagic activities were markedly inhibited at 1:1 and 1:5 proportions with Myrsine guianensis and Jatropha curcas, respectively. Phospholipase and coagulant activities were inhibited with Zanthoxylum monogynum at 1:5 and 1:10 proportions, respectively. Conclusions: The extracts analyzed inhibited the venoms from Bothrops alternatus and Bothrops moojeni due to the presence of molecules with possible antiophidic properties.

RESUMEN Objetivo: evaluar la inhibición de los venenos de las serpientes Bothrops alternatus y Bothrops moojeni por los extractos vegetales de Jatropha curcas, Myrsine guianensis y Zanthoxylum monogynum, en relación con las actividades coagulante, fosfolipasa y hemorrágica. Métodos: Los extractos vegetales se obtuvieron a partir del liofilizado de hojas de Jatropha curca, Myrsine guianensis y Zanthoxylum monogynum. Se realizaron ensayos de inhibición in vitro con un grupo de control positivo (10 μg de veneno crudo), grupos experimentales que recibieron una mezcla del veneno con cada uno de los extractos, en concentraciones de 5 a 500 μg y un grupo de control negativo (500 μg de extracto). Se realizaron ensayos in vivo con grupos de ratones Balb/c (n = 4). A los ratones del grupo de control positivo se les inyectó solamente el veneno (25 μg); a los de los grupos experimentales, una mezcla del veneno con cada uno de los extractos, en concentraciones de 25 a 500 μg; y a los del grupo de control negativo, solamente extracto (500 μg). Resultados: Jatropha curcas y Myrsine guianensis inhibieron significativamente la actividad fosfolipasa a partir de la proporción 1:0,5 (veneno:extracto). Por otro lado, las actividades coagulante y hemorrágica, fueron inhibidas expresivamente a partir de las proporciones 1:1 y 1:5 con Myrsine guianensis y Jatropha curcas, respectivamente. Las actividades fosfolipasa y coagulante fueron inhibidas con Zanthoxylum monogynum a partir de las proporciones 1:5 y 1:10, respectivamente. Conclusiones: Los extractos analizados inhibieron los venenos de Bothrops alternatus y Bothrops moojeni debido a la presencia de moléculas con posibles propiedades antiofídicas.