BACKGROUND Norovirus (NoV) is a major cause of acute gastroenteritis (AGE) worldwide, especially in children under five years. Studies involving the detection and molecular characterisation of NoV have been performed in Brazil, demonstrating its importance as an etiological agent of AGE. OBJECTIVES The objectives of this study were to investigate the frequency of human NoV and to genotype the strains isolated from 0-14-year-old patients of AGE in Manaus, Brazil, over a period of two years. METHODS A total of 426 faecal samples were collected between January 2010 and December 2011. All samples were tested for the presence of NoV antigens using a commercial enzyme immunoassay kit. RNA was extracted from all faecal suspensions and reverse transcription-polymerase chain reaction (RT-PCR) for the NoV-polymerase partial region was performed as a trial test. Positive samples were then subjected to PCR with specific primers for partial capsid genes, which were then sequenced. FINDINGS NoV was detected in 150 (35.2%) faecal samples, for at least one of the two techniques used. NoV was detected in children from all age groups, with the highest positivity observed among the group of 1-2 years old. Clinically, fever was verified in 43% of the positive cases and 46.3% of the negative cases, and vomiting was observed in 75.8% and 70.8% cases in these groups, respectively. Monthly distribution showed that the highest positivity was observed in January 2010 (81.2%), followed by February and April 2010 and March 2011, when the positivity rate reached almost 50%. Phylogenetic analyses performed with 65 positive strains demonstrated that 58 (89.2%) cases of NoV belonged to genotype GII.4, five (7.7%) to GII.6, and one (1.5%) each to GII.7 and GII.3. MAIN CONCLUSIONS This research revealed a high circulation of NoV GII.4 in Manaus and contributed to the understanding of the importance of this virus in the aetiology of AGE cases, especially in a region with such few studies available.

SUMMARY Sapoviruses (SaVs) are responsible for acute gastroenteritis in humans, especially children and the elderly. In Brazil, data on SaVs infections are very limited, especially in Northern Brazil. Here, we investigated the occurrence of SaVs in samples from hospitalized children under ten years old that presented acute gastroenteritis. Positive samples were genotyped and phylogenetic analysis was performed using prototype strains sequences obtained from GenBank database. In total, 156 fecal samples were screened by RT-PCR for SaVs. A positivity rate of 3.8% (6/156) was found in children under three years of age. Four genotypes were detected: GI.I, GI.2 and GII.2?-GII.4?/GII.4, suggesting a possible inter-genotypes recombination. Most infections (83.3%) occurred between August and September. The positivity was similar to that found in other countries and genotyping demonstrated the presence of distinct genotypes. To our knowledge, this is the first study reporting the circulation of SaVs in Manaus, state of Amazonas, Amazon region, Brazil.

<p>Objetivou-se avaliar o efeito de diferentes proporções de concentrado e bagaço de cana-de-açúcar como volumoso exclusivo em dietas para ovinos. Os consumos de matéria seca, matéria orgânica, proteína bruta, carboidratos não fibrosos e nutrientes digestíveis totais expressos em kg/dia, aumentaram linearmente com o aumento da proporção de concentrado na dieta. O mesmo comportamento foi observado para a digestibilidade da matéria seca. O que ocorreu de forma contrária para a digestibilidade da fibra em detergente neutro, que diminuiu com os maiores níveis de concentrado. Com o aumento das proporções de concentrado nas dietas experimentais, foi observado efeito quadrático no tempo despendido com ruminação, alimentação e ócio, e nas concentrações de ácido acético, propiônico e butírico no rúmen. Maiores proporções de concentrado associado ao bagaço de cana-de-açúcar <italic>in natura</italic>podem ser utilizadas em dietas para ovinos.</p>

<p>This study aimed to evaluate the effect of different proportions of concentrate and sugar cane bagasse as roughage exclusive for sheep. The dry matter intake, organic matter, crude protein, non-fibrous carbohydrates and total digestible nutrients in kg/day, increased linearly with increasing proportion of concentrate in the diet. The same behavior was observed for dry matter digestibility. It occurred in a manner contrary to the digestibility of neutral detergent fiber that decreased with the higher levels of concentrate. With the increase of the proportion of concentrate in the diets, quadratic effect was observed to time of rumination, feeding and idleness, and the of concentrations of acetic, propionic and butyric acid in the rumen. Higher proportions of concentrate associated with sugar cane bagasse <italic>in nature</italic>can be used in diets.</p>

Noroviruses (NoVs) are important cause of gastroenteritis in humans worldwide. Genotype GII.4 is responsible for the majority of outbreaks reported to date. This study describes, for the first time in Brazil, the circulation of NoV GII.4 variant Sydney 2012 in faecal samples collected from children aged less than or equal to eight years in Rio Branco, state of Acre, northern Brazil, during July-September 2012.