The aim of this study was to prepare and characterize a ceramic matrix cement of hydroxyapatite (HAp) and galactomannan (Gal) extracted from Adenanthera pavonina L. seed. Three composites were prepared: the solid phases used were 75 wt% HAp and 25 wt% Gal (HAG) both powder adding to composite 0.30 ml distilled water. The composite HAGJET consists of 0.10 mL catalyst commercial JET® (methyl methacrylate and dimethyl-p-toluidine) and 0.20 mL distilled water (see table 1). The HAGLS composite was composed of 60 wt% HAp powder, liquid phase of 40% Gal solution and 0.10 mL of catalyst commercial LS® (phosphoric acid, zinc oxide, aluminum hydroxide and water) (see table 1). The solid phase in each material was dissolved in the liquid corresponding to each composite formed. The phases of HAp and Gal were characterized by X-ray diffraction. The setting time, flow and solubility were tested according to ISO 6876/2001. Furthermore, the amount of water that each material absorbs was determined, as well as their microhardness and morphology. The data were analyzed using 1-way ANOVA with Student’s t-test (p < 0.05). All of the materials exhibited good setting time and flow above that allowed by the ISO (p < 0.05). The HAGLS composite had solubility of less than 3% (p < 0.05), absorbed less water (p < 0.05) and had the highest hardness value. The HAG and HAGLS composites showed roughened surfaces, while the HAGJET exhibited a smooth surface. These results suggest that there is potential to develop a sealer using HAp and Gal.

Este estudo refere-se à caracterização de um compósito a base de hidroxiapatita (Hap) e galactomanana (GAL) extraída da semente de Adenanthera pavonina L. Três compósitos foram preparados: o primeiro compósito é formado por 75% em massa de Hap e 25 % de GAL em fases sólidas utilizando 0,30 mL de água destilada como fase liquida (HAG). O segundo compósito é formado pela mesma proporção de Hap e Gal adicionando 0,20 mL de água destilada e 0,10 mL de catalisador commercial JET® (HAGJET) (ver tabela 1). O terceiro compósito (HAGLS) é compost de 60% em massa de Hap em fase sólida e 40 % de GAL em fase líquida e 0,10 mL de catalisador commercial LS® (ver tabela 1). A fase sólida de cada material foi dissolvida no líquido de cada compósito formado. As fases de Hap e GAL foram caracterizadas por difração de raios-X. Os compósitos foram caracterizados por técnicas de tempo de secagem ou cura, fluxo e solubilidade de acordo com a ISO 6876/2001. Além disso, a quantidade de água absorvida em cada compósito foi observada pelas técnicas de microdureza e morfologia. Os dados foram analisados estatísticamente utilizando o teste t-student com p < 0,05. Todos os compósitos apresentaram bons tempode de cura e fluxo. O HAGLS solubilizou abaixo de 3% com baixa absorção de água e um alto valor de microdureza. HAG e HAGLS apresentaram superficies rugosas, enquanto o HAGJET apresentou superfície lisa. Esses resultados sugerem que os compósitos de Hap e Gal são potencialmentes favoráveis a selantes.

Photosynthesis is one of the main plant metabolic processes affected by copper deficiency and toxicity. The objective of this study was to evaluate the effects of different copper concentrations on transient chlorophyll a fluorescence and modulated fluorescence in sweet potatoes. Sweet potatoes were placed in a hydroponic system and grown for six days with a complete nutrient solution. The plants were then transferred to solutions with different copper concentrations (0.041, 0.082, 0.123 and 0.164 mM) for nine days. The solutions were renewed every three days, and the plants were evaluated at the end of the experiment. Increased copper availability (0.123 and 0.164 mM) positively influenced the structure and functionality of photosystem I (PSI). The increase in Cu availability also reduced the apparent electron transport rate in the PSII [ETR(II)], and the plants treated with 0.082 and 0.123 mM Cu were able to dissipate the excess light energy in the PSII and protect its units (NPQ and qN). A joint analysis of the data shows that high copper concentrations in the nutrient solution lead to reduced photochemical activity of the PSII, increased dissipation of light energy from this same photosystem and increased PSI efficiency in sweet potatoes.

Sorption isotherms of papaya, melon and grape seeds were determined and thermodynamic properties compared. The experiments were carried out at different temperature using the gravimetric method. The Henderson and GAB models were the best which represented the experimental data. Papaya and grape seeds are more stable with larger moisture content than melon seeds at 30°C. Grape seeds presented more resistance to lose water during the dehydration. The differential enthalpy and entropy decreased with increasing moisture content and satisfied the compensation theory. It was found that the sorption process investigated was enthalpy-driven

The surface of gutta-percha cones was evaluated after using five different cutting methods, including a new TipSnip device . The gutta-percha cones were cut off using: 1) TipSnip, 2) a single cut with a scalpel blade using a gauge, 3) two cuts with a scalpel blade using a gauge, 4) a razor blade against a glass slab or 5) scissors. Samples were examined under stereomicroscopy and observed by three highly qualified evaluators. The Kappa coefficient with a 95% confidence interval was used and all scores were tabulated and analyzed statistically using a nonparametric Kruskal-Wallis test with a 5% significance level. Cutting with scissors produced significant irregularities in the cone surface, providing the worst result. TipSnip, two cuts with scalpel blade, and cut with a razor against a glass slab provided the best results. A regular surface on the tips of gutta-percha cones improves apical fit, and may be achieved by means of different cutting methods.

Fue evaluada la superficie de conos de gutapercha después de ser calibrados con cinco diferentes métodos de corte, incluyendo el nuevo dispositivo TipSnip. Los conos de gutapercha fueron cortados con TipSnip, con un corte con hoja de bisturí en regla calibrada, con corte de ida y vuelta con hoja de bisturí en regla calibrada, con hoja de afeitar sobre una platina de vidrio, o con tijeras. Las muestras fueron observadas bajo microscopía estereoscópica y examinadas por tres evaluadores altamente calificados y previamente calibrados utilizado el coeficiente Kappa con intervalo de confianza del 95%; todos los resultados fueron tabulados y analizados estadísticamente mediante el test no paramétrico de Kruskal-Wallis con un nivel de significancia del 5%. El corte con tijeras produjo significativas irregularidades en la superficie del cono, siendo el grupo con peores resultados. El corte con TipSnip, el corte de ida y vuelta con hoja de bisturí, y la hoja de afeitar obtuvieron los mejores resultados. Una superficie regular en la punta de los conos de gutapercha mejora la adaptación apical, y esto puede conseguirse por medio de diferentes métodos de corte.

Este trabalho relata a ocorrência e aspectos epidemiológicos de surtos de pododermatite infecciosa e outras afecções podais em caprinos e ovinos no semiárido paraibano. As propriedades foram inspecionadas quanto à presença de problemas podais e aspectos relacionados às condições ambientais, higiene, locais de pastejo e medidas de controle utilizadas. Nos ovinos a prevalência de lesões podais foi de 19,41% (170/876) e nos caprinos de 17,99% (52/289), variando entre 5,77% e 33,85% nas diferentes propriedades. A pododermatite infecciosa foi a doença mais frequente acometendo 12,1% dos animais examinados (141/1165), sendo a prevalência nos ovinos (13,69%) significativamente maior (p<0,05) do que a nos caprinos (7,27%). A frequência de lesões malignas desta doença em caprinos (9,53%) foi, também, significativamente menor (p<0,05) do que nos ovinos (40,83%). Em uma das propriedades a prevalência de pododermatite infecciosa entre os ovinos Dorper (17,5%) foi significativamente maior do que a nos ovinos da raça Santa Inês (6,79 %), tendo os animais pela pododermatite infecciosa. Dichelobacter nodosus e Fusobacterium necrophorum foram isolados de casos de pododermatite infecciosa. A doença da linha branca foi constatada em 3,95% dos animais, a úlcera de sola em 1,29%, o abscesso do pé em 1,03%, e crescimento excessivo do casco em 0,5%. Os altos índices pluviométricos na época de ocorrência, o pastejo em áreas úmidas, os solos argilosos com pouca drenagem, os terrenos pedregosos, o encerramento em currais durante a noite e a introdução de animais doentes foram considerados fatores predisponentes para a ocorrência dos surtos.

This paper reports the occurrence and epidemiology of outbreaks of foot rot and other foot diseases in goats and sheep in the semiarid region of Paraíba, northeastern Brazil. Four farms were inspected for the presence of foot lesion in sheep and goats and for environmental conditions, general hygiene, pastures, and disease control measures. The prevalence of foot lesions was 19.41% (170/876) in sheep and 17.99% (52/289) in goats, ranging between 5.77% and 33.85% in different farms. Foot rot was the most common disease, affecting 12.1% of the animals examined (141/1165), but with significantly higher (p<0.05) prevalence in sheep (13.69%) than in goats (7.27%). The frequency of malignant foot rot was also significantly lower (p<0.05) in goats (9.53%) than in the sheep (40.83%). On one farm, Dorper sheep showed significantly higher (p<0.05) prevalence of foot rot (17.5%) than Santa Inês sheep (6.79%), and the number of digits affected was also higher in the former. Dichelobacter nodosus and Fusobacterium necrophorum were isolated from cases of foot rot. White line disease was found in 3.95% of the animals, sole ulcers in 1.29%, foot abscess in 1.03% and hoof overgrowth in 0.5%. The high rainfall at the time of occurrence, grazing in wetlands, clay soils with poor drainage, presence of numerous stony grounds, closure of the flocks in pens at night, and introduction of affected animals were considered predisposing factors for the occurrence of foot diseases.

Crescimento e características de carcaça do jundiá (Rhamdia quelen) foram avaliados após 60 dias de alimentação com diferentes proporções de amilose:amilopectina na dieta. Foram criados 240 peixes (peso inicial de 34,3±4,3g) em sistema de recirculação de água constituído de 12 unidades experimentais de 280L. Foram testadas três dietas com as proporções amilose:amilopectina de: T417 = com 26% de amilose, T416 = com 16% de amilose e TMOCHI = com 0% de amilose. Os peixes foram alimentados duas vezes por dia, às 8h e 30min e às 16 horas. A cada 15 dias, foram feitas biometrias para ajuste da quantidade de alimento ofertado. A proporção amilose:amilopectina no amido das dietas não influenciou o crescimento, os rendimentos de carcaça e a composição corporal dos peixes. Conclui-se que o jundiá tem potencial para utilizar fontes de amido compostas por até 26% de amilose sem alterar a digestibilidade dos nutrientes e a deposição de gordura.

The growth and carcass traits of jundiá (Rhamdia quelen) after 60 days being fed with different amylose:amylopectin ratio in diet were evaluated. Two hundred and forty fish (initial weight of 34.3±4.3g) were raised in water re-use system with 12 units of 280L. Three diets were tested with amylose:amylopectin ratios: T417 = 26% amylose, T416 = 16% amylose and TMOCHI = 0% amylose. The fish were fed twice a day at 8:30 and 16:00h. Every 15 days the amount of food offered was ajusted. The amylose:amylopectin ratio in the starch of the diet did not affect growth, carcass and body composition of fish. This demonstrates that the jundiá has the potential to use starchy sources consisting of up to 26% amylose without altering the digestibility of nutrients and fat deposition.

Em razão de estudos e especulações envolvendo a questão da soja RR sob aplicação de glyphosate, são necessárias investigações que permitam esclarecer melhor essa situação. O presente trabalho teve por objetivo avaliar o desempenho agronômico e os teores de óleo e proteínas sob aplicação do herbicida glyphosate na cultura da soja transgênica. Para isso, foi desenvolvido um ensaio em delineamento em blocos casualizados, com quatro repetições, em que os tratamentos avaliados consistiram da pulverização foliar contendo glyphosate, em doses crescentes, aplicadas nos estádios V6 e R 2 . As variáveis avaliadas foram: altura média das plantas, número de vagens por planta, massa de mil grãos e produtividade, assim como os teores de óleo e proteínas. Verificou-se que o glyphosate, especialmente quando usado em R 2, pode comprometer o desempenho agronômico e os teores de proteínas.

Previous studies and speculation involving the behavior of Roundup Ready soybean under glyphosate application requires further investigations to clarify this issue. This study aimed to evaluate the agronomic performance and oil and protein contents of transgenic soybean culture under glyphosate application. Thus, an assay was carried out in a completely randomized block design, with four replications. Treatments consisted of foliar sprays in increasing doses of glyphosate applied at stages V6 and R2. The variables evaluated were: plant height, number of pods per plant, thousand grain weight and yield, as well as oil and protein contents. It was verified that glyphosate, especially when used in R2, can compromise both the agronomic performance and protein contents.

The objective of this work was to verify the MS production and nutritive value of five clones of Pennisetum. The samples were taken from an utile area of 2,0 m² in each plot. After clipping the forage to standarize, four cuts were performed with 60-d intervals. In each plot, three tillers were oven-dried and grinded for laboratory analysis. The following response variables were determined: dry matter concentration (MS), crude protein (PB), neutral detergent fiber (FDN), acid detergent fiber (FDA), ether extract (EE), mineral matter (MM), in vitro dry matter digestibility (DIVMS), total carbohydrates (CHOT), and non-fibrous carbohydrates (CNF). The evaluated genotypes presented average MS yield of 1.7 and 2.1 t/ha/ cut, for leaf blade and stem, respectively. Average nutritive value was 21.1 and 15.1% for MS; 9.1 and 5.3% for PB; 73.2 and 77.7% for FDN; 40.3 and 47.4% for FDA; 9.6 and 11.6% for MM; 1.9 and 0.7% for EE; 56.2 and 56.3% for DIVMS; 79.3 and 82.4% for CHOT; and 11.4 and 9.7% for CNF, in leaf blades and stem, respectively. The clones presented medium to high heritability, similar biomass production and nutritive value, when harvested at 60 days of regrowth in the conditions of the forest zone of Pernambuco.

Objetivou com este trabalho verificar a produção e valor nutritivo de cinco genótipos de Pennisetum (IRI-381, Venezuela, Elefante B, HV-241 e Hexaplóide) sob corte na Zona da Mata Seca de Pernambuco. As amostras foram obtidas de uma área útil de 2,0 m², num delineamento em blocos casualizados com 6 repetições. Após o corte de uniformização, efetuaram-se quatro cortes a intervalos de 60 dias. Foi tomada uma amostra de três perfilhos de cada parcela. Foram avaliados os percentuais de matéria seca (MS), proteína bruta (PB), fibra em detergente neutro (FDN), fibra em detergente ácido (FDA), extrato etéreo (EE), matéria mineral (MM), digestibilidade in vitro da matéria seca (DIVMS) carboidratos totais (CHOT) e carboidratos não fibrosos (CNF). Os genótipos avaliados apresentaram rendimento médio de MS de lâminas foliares de 1,7 t/ha/corte e de colmos de 2,1 t/ha/corte, não havendo diferença entre clones (p>0,05). Nas lâminas foliares e nos colmos, respectivamente, observou-se 21,1 a 15,1% para MS; 9,1 a 5,3% para PB; 73,2 a 77,7% para FDN; 40,3 a 47,4% para FDA; 9,6 a 11,6% para MM; 1,9 a 0,7% para EE; 56,2 a 56,3% para DIVMS; 79,3 a 82,4% para CHOT e 11,4 a 9,7% para CNF. Os clones apresentaram de média a alta herdabilidade e rendimento forrageiro e valor nutritivo semelhantes, quando manejados aos 60 dias de idade nas condiçoes da zona da mata seca de Pernambuco.

Avaliou-se o efeito da ractopamina e de diferentes métodos de formulação de dietas sobre o desempenho e as características de carcaça de leitoas em terminação. Foram utilizadas 60 leitoas, híbridas comerciais, distribuídas em delineamento experimental de blocos ao acaso, em esquema fatorial 2x3, sendo dois níveis de ractopamina e três métodos de formulação de dietas. Houve interação de métodos de formulação versus ractopamina para ganho de peso diário, peso corporal, conversão alimentar e taxa de deposição de carne magra (TDCM) na avaliação de carcaça in vivo por ultrassom. A dieta formulada com alta proteína bruta proporcionou maior consumo de dieta (CRD), de lisina (CLD), ganho de peso diário (GPD) e peso corporal final. A suplementação de ractopamina não influenciou o CRD e o CLD, entretanto aumentou o GPD. O método de formulação da dieta não influenciou as características de carcaça aos 28 dias, enquanto a suplementação de ractopamina reduziu a espessura de toucinho, aumentou a profundidade de lombo e o rendimento de carne magra. A dieta formulada com alta proteína bruta proporcionou melhores resultados de desempenho e taxa de deposição de carne magra na carcaça de leitoas em terminação, suplementadas com ractopamina.

The effect of ractopamine supplementation and feed formulation methods was evaluated on performance and carcass characteristics of finishing gilts. Sixty animals were allotted in a completely randomized block experimental design, in a 2x3 factorial scheme - ractopamine (0 and 5ppm) X three feed formulation methods. Interactions between feed formulation method and ractopamine for daily weight gain (DWG), body weight, feed:gain (DFG), and lean carcass meat deposition (LCMD) were by in vivo carcass evaluation using ultrasound observed. Daily feed intake (DFI), lysine intake (DLI), DWG, and final body weight were higher for diets with high protein level. Ractopamine supplementation did not affect feed intake, and lysine intake, but increased daily weight gain. Feed formulation method affected carcass characteristics recorded at 28 days, while ractopamine supplementation reduced backfat thickness, increased muscle depth, and lean carcass proportion of the gilts. Ractopamine improved performance and carcass characteristics of finishing gilts and feed formulation methods affected performance and carcass characteristics. Better performance and higher lean carcass deposition were observed for finishing gilts fed high protein level diets supplemented with ractopamine.

Procedeu-se à criopreservação do sêmen de oito tourinhos Gir Leiteiro, com idade média de 25 meses, pré-selecionados para elevada pontuação (média 84,4±5,6) na classificação andrológica por pontos (CAP), em dois diferentes diluidores: um à base de lactose-gema-glicerol e outro à base de lecitina de soja. As curvas de resfriamento e congelação foram padronizadas com o auxílio da máquina CRYOGEN®. Os parâmetros pós-congelação avaliados no sêmen submetido aos dois diluidores - motilidade, vigor, defeitos maiores, menores e totais, defeitos de acrossoma, cauda dobrada, reação ao teste hiposmótico (Thos) e células normais - foram comparados aos do sêmen fresco, exceto para Thos e entre eles. O sêmen de todos os animais foi congelado com êxito no diluidor lactose-gema-glicerol. Houve diferença (P<0,05) em todas as variáveis analisadas no sêmen fresco e pós-congelado, exceto para defeitos maiores. Entre diluidores, houve diferença (P<0,05) para motilidade, vigor, cauda dobrada e Thos. Estes resultados indicam que a seleção pelo CAP médio >80 é um bom índice para selecionar touros com maior taxa de espermatozoides viáveis pós-congelação

Semen cryopreservation from eight young dairy Gyr bulls was performed using two different semen extenders. Bulls aging 25 months old and pre-selected for a high average score (84.4±5.6 in a 0-100 scale) in Zebu breeding soundness evaluation (BSE) composed the experimental group. Extenders were based on lactosis-egg yolk-glycerol and soya lecithin. Chilling and freezing curves were standardized by CRYOGEN® machine. Post-thaw features evaluated in semen frost in both extenders - motility, vigor, major, minor and total deffects, morphological alteration in acrossome, bent tail, reative cells to hyposmotic sweeling test (Thos), and normal cells - were compared to the ones in the fresh ejaculate (except Thos) and among them. It was possible to freeze semen from all animals in the lactosis-egg yolk-glycerol extender. There were difference (P<0.05) in all analyzed features between fresh and cryopreserved semen, except for major deffects. Between extenders, there were differences (P<0.05) in motility, vigor, bent tail, and Thos. All bulls had successfull semen freezability. These results sustain that pre-selection for high BSE values (average >80 points) is a good index to identify bulls with good post semen - thaw features. However, the choice of the extender is critical for obtaining acceptable results

The fluoroscopy-guided renal arteriography was evaluated in order to be used as an auxiliary method in investigations and as a way to experimentally induce kidney diseases in swine. The technique was effective to obtain sharp images as well as to determine the area of renal irrigation. Despite its easiness, trained professionals are required to perform it.

O sisal (Agave sisalana Perrine ex Engelm.) é a principal fonte de extração de fibras duras vegetais do mundo. No Brasil, o cultivo ocupa extensa área de solos pobres na região semi-árida dos Estados da Bahia, Paraíba e Rio Grande do Norte, em regiões com escassa ou nenhuma alternativa para exploração de outras culturas. Tendo em vista a propriedade inibitória dos extratos vegetais sobre o desenvolvimento de fungos, avaliou-se o efeito inibitório do extrato vegetal de alho (Allium sativum L.) sobre o fungo Aspergillus niger isolados da cultura do sisal. O extrato vegetal foi incorporado ao meio BDA fundente, aproximadamente 45ºC, nas concentrações 0, 500, 1000, 2000, 50000, 10000 e 50000 mg L-1. Os resultados demonstraram que a dose de 50000 mg L-1 de alho foi eficiente na inibição do crescimento do fungo Aspergillus niger "extraídos" da cultura do sisal.

Sisal (Agave sisalana Perrine ex Engelm.) is the main source of plant hard fiber extraction in the world. In Brazil, its cultivation occupies a large area of poor soils in the semi-arid region of Bahia, Paraiba and Rio Grande do Norte States, and in regions with scarce or no alternative to explore other crops. Based on the inhibitory property of plant extracts on fungal development, the inhibitory effect of garlic (Allium sativum L.) extract was evaluated on the fungus Aspergillus niger isolated from sisal crop. The plant extract was incorporated into melting PDA medium around 45ºC, at the following concentrations: 0, 500, 1000, 2000, 50000, 10000, and 50000 mg L-1. (OBS: M. Elena, há duas doses repetidas; creio que a primeira seja 5000). The concentration 50000 mg L-1 garlic extract was effective in inhibiting the growth of the fungus Aspergillus niger extracted from sisal crop.

A numeric formulation is presented based on a combination of fracture mechanics and continuum damage mechanics to treat the problem of the crack propagation in elastic regime, under the effect of a mechanical action defined by a static load applied in the tensile mode and of an environmental action characterized by hydrogen diffusion into the lattice. The equations that describe the evolution of the variables at the crack tip form a non-linear system of ordinary differential equations that is solved using the 4th order Runge-Kutta method. The results show the influence of hydrogen on the decrease of the time at which cracks starts and propagates, which is consistent with macroscopic observations of the hydrogen embrittlement phenomenon.

Se presenta una formulación numérica basada en una combinación de la mecánica de la fractura y de la mecánica del daño continuo para tratar el problema de la propagación de una fisura en régimen elástico, bajo el efecto de una acción mecánica definida por una carga estática aplicada en el modo de fractura I y de una acción ambiental caracterizada por la difusión de hidrógeno a través de la estructura cristalina del material. Las ecuaciones que describen la evolución de las variables en la punta de la fisura forman un sistema no lineal de ecuaciones diferenciales ordinarias que es resuelto con el método de Runge-Kutta de 4º orden. Los resultados obtenidos muestran la influencia del hidrógeno en la disminución del tiempo de inicio y propagación de fisuras, lo que es consistente con las observaciones macroscópicas del fenómeno de fragilización por hidrógeno.

There is evidence showing a close relationship between diet and the occurrence of non-communicable chronic diseases. The present study assessed food consumption in a 2002/2004 cohort of young adults born in 1978/79 in Ribeirão Preto, SP, Brazil. The composition of the habitual diet consumed by a sample of 2063 individuals aged 23-25 years was analyzed using a validated semi-quantitative food frequency questionnaire based on studies of prevention of non-communicable chronic diseases. The Dietsys software was used for dietary calculations. In terms of WHO/2003 recommendations, there was a high mean daily consumption of energy from fat (consumption: 35.4%; recommendation: 15-30%), a low mean intake of energy from carbohydrates (47.5%; 55-75%) and a low mean consumption of total fibers (15.2 g; >25 g). Mean intake of energy from fatty acids (10%; <10%) and protein (15.6%; 10-15%) was within recommended limits. When compared to the recommendations of the food pyramid adapted to the Brazilian population, adequate intake was observed only regarding the meat group (consumption: 1.9 portions; recommended: 1-2). There was a low consumption of vegetables (2.9; 4-5), fruits (1.2; 3-5), breads (3.6; 6-9), and dairy products (1.7; 3), with excessive fat and sugar intake (5.7; 1-2). We conclude that the inadequate food consumption observed in this young population may be associated with the development of excess weight and may contribute to the triggering of non-communicable chronic diseases.

OBJETIVO: Estudar aspectos funcionais e morfológicos dos auto-implantes esplênicos e do pólo inferior do baço de ratos, tratados ou não com oxigênio hiperbárico no pós-operatório, e a sobrevida desses animais. MÉTODOS: Foram operados 78 ratos, machos, Wistar, pesando entre 192 g e 283 g ( M.A 238,3 ± 9,6) , distribuídos aleatoriamente em três grupos : 1-(n=20) , manipulação do baço; 2-(n=36), auto-implante esplênico; 3(n= 22), esplenectomia subtotal com preservação do pólo inferior. Cada grupo foi dividido em dois subgrupos: a- não submetido à oxigenoterapia hiperbárica: 1a(n=10), 2a(n=21), 3a(n= 13); b- submetido: 1b(n=10), 2b(n=15), 3b(n=9). O sangue foi colhido para dosagem dos lípides e imunoglobulinas e contagem das plaquetas e dos corpúsculos de Howell-Jolly no pré-operatório e 11 dias após a cirurgia. O baço e os remanescentes foram retirados para estudo histológico. RESULTADOS: O número de animais sobreviventes foi significantemente maior nos grupos 1(p<0,01) e 3(p<0,05) em relação ao subgrupo 2a. O colesterol total e a fração LDL aumentaram significantemente nos subgrupos 2a (p<0,01) e 3a(p<0,05), e não alteraram nos subgrupos 2b e 3b. A IgM caiu mais significantemente no grupo 2 que no 3. O aumento do número de plaquetas e o aparecimento dos corpúsculos de Howell Jolly foi menor no subgrupo 2b que no 2a, no grupo 3 que no grupo 2. O aspecto macro e microscópico no subgrupo 2b foi mais viável que no subgrupo 2a, e o do grupo 3 mais viável que no grupo 2. A sobrevida dos animais com todo o baço ou com o pólo inferior foi mais freqüente que a dos animais com auto-implantes. CONCLUSÕES: A função e a viabilidade de todo o baço ou do pólo inferior foram melhores que a dos auto-implantes. A oxigenoterapia hiperbárica contribuiu para aumentar a freqüência de sobrevida dos animais com auto-implantes, para melhorar a função e a viabilidade dos auto-implantes e a função do pólo inferior, e não interferiu nos animais com baço íntegro.

PURPOSE: To assess functional and morphological aspects of spleen auto-implants and of the splenic inferior pole of rats, post-operatively treated or not with hyperbaric oxygen, as well as the survival of these animals, were studied. METHODS: Seventy-eight male Wistar rats, weighing between 192 and 283 g ( 238,3 ± 9,6g), were randomly distributed into three groups: Group1-(n=20), spleen manipulation; group 2-(n=36), spleen auto-implantation; group3-(n= 22), subtotal splenectomy preserving the inferior pole. Each group was subdivided as follows: subgroup a, not submitted to hyperbaric oxygen therapy: 1a(n=10), 2a(n=21), 3a(n= 13); subgroup b, submitted to the therapy: 1b(n=10), 2b(n=15), 3b(n=9). Blood was collected pre-operatively and 11 days after surgery, for the estimation of lipids and immunoglobulins and the counting of platelets and Howell-Jolly corpuscles. The spleen and remains were taken for histological study. RESULTS: The number of surviving animals was significantly higher in groups 1(p<0,01) and 3(p<0,05) relative to those of subgroup 2a. Total cholesterol and the LDL fraction increased significantly in subgroup 2a (p<0,01) and 3a (p<0,05), and remained unaltered in subgroups 2b e 3b. IgM decreased more significantly in subgroup 2 than in subgroup 3 (p<0,001 vs p<0,01). The increase of platelet numbers and the appearance of Howell Jolly corpuscles was smaller in subgroup 2b compared to subgroup 2a , and in group 3 compared to group aqui-> 2. The macro and microscopic appearance in subgroup 2b were more viable than in subgroup 2a, and that of group 3 more viable than in group 2. The survival of the animals carrying their whole spleen or its inferior pole was more frequent than that of the auto-implanted animals. CONCLUSION: Functionality and viability of the whole spleen or of its inferior pole, were better than in the auto-implanted animals. Hyperbaric oxygentherapy contributed to increased survival frequency of auto -implanted animals, and to improve the functionality and viability of the auto-implants and the function of the inferior splenic pole, and did not interfere in animals carrying their whole spleen.