ABSTRACT Chrysanthemum (Dendranthema grandiflora) is the second most produced and commercialized ornamental plant in the world. Measuring leaf area through non-destructive methods is fundamental for studies on its growth and production. The estimation of leaf area by linear dimensions of the leaves can be a strategy for this purpose. The objective of this study was to find allometric equations to estimate the leaf area of chrysanthemum. The linear, linear without intercept, quadratic, cubic, power, and exponential regression models were used for the analysis. The choice of equations was based on the highest coefficients of determination. The non-destructive method using allometric models has accuracy for estimating the leaf area (LA) of chrysanthemum from the product between leaf length (L) and leaf width (W). The LA of chrysanthemum can be estimated using the equation ŷ = 0.6611*LW0.9490 (L - leaf length; W - leaf width). This equation will allow researchers and producers to determine leaf area non-destructively.

RESUMO O crisântemo (Dendranthema grandiflora) é a segunda planta ornamental mais produzida e comercializada no mundo. A medição da área foliar por métodos não destrutivos é fundamental para estudos sobre seu crescimento e produção. A estimativa da área foliar por dimensões lineares das folhas pode ser uma estratégia para este fim. O objetivo deste estudo foi encontrar equações alométricas para estimar a área foliar do crisântemo. Os modelos de regressão linear, linear sem intercepto, quadrático, cúbico, potência e exponencial foram utilizados para a análise. A escolha das equações foi baseada nos maiores coeficientes de determinação. O método não destrutivo por meio de modelos alométricos tem acurácia para estimar a área foliar (AF) do crisântemo a partir do produto entre o comprimento da folha (C) e a largura da folha (L). A AF do crisântemo pode ser estimada pela equação ŷ = 0,6611*CL0,9490 (C - comprimento da folha; L - largura da folha). Essa equação permitirá que pesquisadores e produtores determinem a área foliar de forma não destrutiva.

Abstract Introduction: Optimized allocation of medical resources to patients with COVID-19 has been a critical concern since the onset of the pandemic. Methods: In this retrospective cohort study, the authors used data from a Brazilian tertiary university hospital to explore predictors of Intensive Care Unit (ICU) admission and hospital mortality in patients admitted for COVID-19. Our primary aim was to create and validate prediction scores for use in hospitals and emergency departments to aid clinical decisions and resource allocation. Results: The study cohort included 3,022 participants, of whom 2,485 were admitted to the ICU; 1968 survived, and 1054 died in the hospital. From the complete cohort, 1,496 patients were randomly assigned to the derivation sample and 1,526 to the validation sample. The final scores included age, comorbidities, and baseline laboratory data. The areas under the receiver operating characteristic curves were very similar for the derivation and validation samples. Scores for ICU admission had a 75% accuracy in the validation sample, whereas scores for death had a 77% accuracy in the validation sample. The authors found that including baseline flu-like symptoms in the scores added no significant benefit to their accuracy. Furthermore, our scores were more accurate than the previously published NEWS-2 and 4C Mortality Scores. Discussion and conclusions: The authors developed and validated prognostic scores that use readily available clinical and laboratory information to predict ICU admission and mortality in COVID-19. These scores can become valuable tools to support clinical decisions and improve the allocation of limited health resources.

Black mold, a disease caused by the fungus Pilgeriella anacardii Arx & Müller, affects cashews (Anacardium occidentale). Some cashew clones are more resistant to the pathogen; however, little is known about the chemical profile responsible for this trait. The investigation of volatile organic compounds (VOCs) from leaves of dwarf cashew clones resistant (BRS 226 and BRS 265) and susceptible (CCP 76 and BRS 189) to the pathogen was carried out. Leaves were collected during the months of disease incidence and decline (March to July 2019, Brazil), and VOCs were analyzed by gas chromatography-mass spectrometry (GC-MS) combined with chemometric tools. The GC-MS analysis tentatively identified 96 compounds. Partial least squares discriminant analysis (PLS-DA), orthogonal partial least squares discriminant analysis (OPLS DA), hierarchical cluster analysis (HCA), and ROC curves analysis were useful in dividing VOCs into distinct resistance and associated chemical susceptibility groups for different clones. The VOCs in the leaves of the resistant clones were identified as alcohols and aldehydes containing six carbons: (E)-hex-2-enal, hex-3-en-1-ol, (Z)-hex-2-en-1-ol, (E)-hex-2-en-1-ol, and hexan-1-ol. Moreover, α-pinene, pseudolimonene, α-phellandrene, β-myrcene, sylvestrene, β-cis-ocimene, methyl salicylate, myrtenol, α-copaene, γ-muurolene, germacrene D, valencene, and germacrene B were also detected in these samples and may be candidate chemical biomarkers for cashew resistance to P. anacardii.

RESUMO Congea tomentosa é uma trepadeira indicada para cobertura de mandris, grades e cercas. A determinação da área foliar é útil para entender a relação planta-ambiente e facilitar estudos agronômicos sobre transpiração, necessidade de água, interceptação de luz e atividade fotossintética. O objetivo deste estudo foi obter uma equação alométrica para estimar a área foliar de C. tomentosa através da medição das dimensões foliares. As análises foram realizadas em 200 folhas de diferentes formas e tamanhos de 10 plantas adultas escolhidas aleatoriamente cultivadas em condições de campo. O comprimento da folha, a largura da folha, o produto do comprimento pela largura e a área foliar foram determinados. Modelos de regressão linear, linear sem intercepto, quadrático, cúbico, potência e exponencial foram utilizados para estimar a área foliar. O coeficiente de determinação, índice de concordância de Willmott, critério de informação de Akaike, raiz do quadrado médio do erro e índice BIAS foram usados para determinar o melhor modelo. A área foliar de C. tomentosa pode ser satisfatoriamente estimada por meio de um método não destrutivo que utiliza medidas de dimensões foliares. A equação ŷ = 0,63 × LW (Folha: L = comprimento, W = largura) estima a área foliar de C. tomentosa de forma prática e rápida, com 99,15% de precisão. A estimativa da área foliar de C. tomentosa utilizando modelos estatísticos é menos dispendiosa e de fácil acesso aos pesquisadores e produtores desta planta.

ABSTRACT Congea tomentosa is a climbing plant suitable for covering arbors, railings, and fences. Leaf area determination is useful in understanding the plant-environment relationship and facilitating agronomic studies on transpiration, water requirement, light interception, and photosynthetic activity. The objective of this study was to obtain an allometric equation to estimate the leaf area of C. tomentosa by measuring the leaf dimensions. Analyses were performed on 200 leaves of different shapes and sizes from 10 randomly chosen adult plants grown under field conditions. The leaf length, leaf width, product length and width, and leaf area were determined. Linear, linear without intercept, quadratic, cubic, power, and exponential regression models were used to estimate the leaf area. The coefficient of determination, Willmott’s concordance index, Akaike information criterion, root mean square error and BIAS index were used to determine the best model. The leaf area of C. tomentosa can be satisfactorily estimated using a non-destructive method that uses measurements of leaf dimensions. The equation ŷ = 0.63 × LW (Leaf: L = length, W = width) estimates the leaf area of C. tomentosa in a practical and fast way, with 99.15% of precision. Estimation of the leaf area of C. tomentosa using statistical models is less expensive and easily accessible to researchers and producers of this plant.

ABSTRACT Sugarcane-associated nematodes (Saccharum spp.) can reduce productivity up to 50%. Through the survey, it was possible to identify the main nematodes that occur in a certain region as a tool for designing the best management and control strategies. The aim of this study was to characterize the population of nematodes associated with sugarcane in the North Central, North Pioneiro and Northwest mesoregion of the state of Paraná, Brazil, quantify the nematode genera associated with the crop and identify the species of Pratylenchus and Meloidogyne. A total amount of 89 soil and root composite samples were collected in nine municipalities. Nematodes were extracted and counted in a Peters counting chamber under an optical light microscope. Morphological description followed identification keys. Pratylenchus spp. were identified by morphological characteristics; Meloidogyne spp. were identified by morphological characteristics and isoenzyme electrophoresis. Twelve genera of nematodes associated with sugarcane were identified: Pratylenchus, Meloidogyne, Helicotylenchus, Xiphinema, Mesocriconema, Trichodorus, Aphelenchus, Hoplolaimus, Tylenchus, Tylenchorhynchus, Ditylenchus, and Paratrichodorus. The genera Pratylenchus and Meloidogyne were found with the highest frequencies in the roots. Among the species of Pratylenchus, P. zeae and P. brachyurus were found, with P. zeae being the most frequent. Among the Meloidogyne species, only M. javanica was found. These results are essential to aid decision making in the management of phytonematodes, mainly in the development of new control strategies and in directing genetic breeding programs for development of sugarcane cultivars for the Paraná state.

RESUMO Objetivo: Realizar um levantamento sobre a interferência dos medicamentos nos exames laboratoriais. Métodos: Utilizamos a Relação Municipal dos Medicamentos Essenciais (Remune) de um município de médio porte do estado de Minas Gerais e as seguintes fontes de informações: Dynamed, Micromedex® e Nursing Reference Center. Resultados: Observamos que a maioria (67,7%) dos medicamentos da Remune pode interferir em um ou mais exames laboratoriais; entre eles, as principais classes de medicamentos são os diuréticos, betabloqueadores, betalactâmicos, sulfonamidas, macrolídeos, antidepressivos tricíclicos e antidepressivos inibidores seletivos da recaptação de serotonina. Conclusão: É muito importante divulgar o conhecimento a respeito dessas interferências para que os profissionais da área da saúde possam identificar as possíveis alterações nos exames, reduzindo erros analíticos e diagnósticos e monitoramentos equivocados. Portanto, faz-se necessária a educação continuada sobre as interferências de medicamentos nos exames laboratoriais.

ABSTRACT Objective: Conduct a survey on the interference of drugs in laboratory tests. Methodology: To identify the interference of each medication in laboratory tests, the Municipal List of Essential Medicines (Remume) of a medium-sized municipality in the state of Minas Gerais was used along with the following sources of information: Dynamed, Micromedex® and Nursing Reference Center. Results: We observed that the majority (67.7%) of Remume drugs can interfere in one or more laboratory tests; among them, the main classes of drugs are diuretics, beta blockers, ß-lactams, sulfonamides, macrolides, tricyclic antidepressants and selective serotonin reuptake inhibitors antidepressants. Conclusion: It is extremely important to disseminate knowledge about these interferences so that health professionals are alert and know how to identify the possible changes, reducing analytical errors and subsequently misdiagnosis and erroneous monitoring. Continued education on drug interference in laboratory tests is equally important.

RESUMEN Objetivo: Llevar a cabo una encuesta acerca de la interferencia de los medicamentos en las pruebas de laboratorio. Métodos: Usamos la Relación Municipal de Medicamentos Esenciales (Remume) de una municipalidad mediana en el estado de Minas Gerais y las siguientes fuentes de información: Dynamed, Micromedex® y Nursing Reference Center. Resultados: Observamos que la mayor parte (67,7%) de los medicamentos de Remume puede interferir en una o más pruebas de laboratorio; entre ellos, las principales clases son los diuréticos, beta bloqueadores, betalactámicos, sulfonamidas, macrólidos, antidepresivos tricíclicos y antidepresivos inhibidores selectivos de la recaptación de serotonina. Conclusión: Es muy importante divulgar el conocimiento respecto a esas interferencias para que los profesionales de la salud puedan identificar las posibles alteraciones en las pruebas, reduciendo errores analíticos y diagnósticos y monitoreos equivocados. Por lo tanto, es necesaria una educación continuada acerca de las interferencias de medicamentos en las pruebas de laboratorio.

Biodiesel made from oils is rich in polyunsaturated fatty acids methyl esters, and, consequently, presents high tendency to oxidize. Biodiesel made from fats, on the other hand, is mainly comprised by saturated chains. Although it is highly stable, they easily crystalize at low temperatures. Blends from both can exhibit optimized properties. In this work, oxidative stability, expressed by induction period (IP), and cold filter plugging point (CFPP) of blends from palm, palm kernel, babassu, tallow or lard biodiesels with soy one were measured. Biodiesel samples were prepared by two steps transesterification methodology with KOH catalysis followed by H2SO4. IP increases, as the fat biodiesel content in the blend is higher, as expected. Blends comprised by 60% of fat biodiesel by volume had their IP increased from 1.5 (lard) to 2 times (palm kernel), compared to soy one. At this level, IP was found 8 h or higher, except for palm, which 80% was required. CFPP was observed at 8 °C or lower for the blends containing up to 60% of all the fat biodiesel, except for the palm one, which a 20% content must not be surpassed. Palm kernel 80% biodiesel blend reached the Brazilian specification, that is, IP higher than 12 h and CFPP lower than 5 °C.

ABSTRACT Powdery mildew, caused by the fungus Pseudoidium anacardii, is currently the most important cashew disease, affecting leaves, inflorescences, and fruits. However, there is a lack of detailed reports associating the effects of P. anacardii to cashew yield. Therefore, the objective of this study was to determine the damage caused by powdery mildew to BRS 189 cashew clone nut, kernel, and peduncles. One experiment was conducted at the Embrapa Agroindústria Tropical Experimental Field in Pacajus, state of Ceará, Brazil, from July to December 2014, with BRS 189 cashew clone, with eight sulfur doses needed for achieving a disease gradient, distributed in a randomized block with four replications. The incidence (%) and severity of powdery mildew in cashew plants were estimated in a scale ranging from 0 to 4, and then correlated with plant yield and biometric characteristics of cashew nuts, kernels, and peduncles. The results showed that powdery mildew did not reduce productivity of nuts on this clone, although it caused a reduction in the mass and size of the kernels. On the other hand, powdery mildew affected mass, size, and quality of cashew peduncles at the lack of control.

RESUMO: O presente estudo foi conduzido com o objetivo de determinar as causas de fotossensibilização em ruminantes e equídeos no Nordeste do Brasil, através da revisão dos laudos de exames arquivados no Laboratório de Patologia Veterinária da Universidade Federal da Paraíba. Durante os quatro anos do estudo foram diagnosticados 22 surtos de fotossensibilização, incluindo 11 surtos de fotossensibilização primária e oito surtos de fotossensibilização hepatógena. A intoxicação por Froelichia humboldtiana foi a principal causa de fotossensibilização e a única causa de fotossensibilização primária. As espécies mais gravemente afetadas por fotossensibilização primária foram os asininos, caprinos, bovinos e ovinos, mas os equinos e mulas também são afetados. A intoxicação por Brachiaria decumbens foi a principal causa de fotossensibilização hepatógena e afetou apenas os ovinos e bovinos. Outras plantas associadas com fotossensibilização hepatógena incluíram Enterolobium contortisiliquum e Lantana camara. Dermatite alérgica foi diagnosticada em dois rebanhos ovinos e em um cavalo. Os animais tinham lesões crônicas, caracterizadas por alopecia, crostas e hiperpigmentação no topo da cabeça, ao redor dos olhos (ovinos) e nos membros (equino). O prurido foi o principal sinal clínico observado nos casos de fotossensibilização primária e hipersensibilidade à picada de insetos.

ABSTRACT: The study was conducted to determine the causes of photosensitization in ruminants and equidae in northeastern Brazil through a review of the files at the Laboratório de Patologia Veterinária of Universidade Federal da Paraíba. During four years of the study 22 outbreaks of photosensitization were diagnosed, including 11 outbreaks of primary photosensitization and eight outbreaks of hepatogenous photosensitization. Poisoning by Froelichia humboldtiana was the main cause of photosensitization, and the only cause of primary photosensitization. The most severely affected animals by primary photosensitization are donkeys, goats, cattle and sheep, but horses and mules may also be affected. Poisoning by Brachiaria decumbens was the main cause of hepatogenous photosensitization, and affected only sheep and cattle. Other plants associated with hepatogenous photosensitization in cattle include Enterolobium contortisiliquum and Lantana camara. Allergic dermatitis was diagnosed in two flocks of sheep and in a horse. The animals had chronic lesions characterized by areas of alopecia, crusts and hyperpigmentation on the head, around the eyes (sheep) and at the legs (horse). Itching was the main clinical sign in cases of primary photosensitization and insect hypersensitivity.

ABSTRACT In general, Passiflora species have been reported for their folk medicinal use as sedative and anti-inflammatory. However, P. caerulea has already been reported to treat pulmonary diseases. Severe pulmonary tuberculosis, generally caused by Mycobacterium tuberculosis strains resistant to multiple drugs, can lead to deleterious inflammation and high mortality, encouraging new approaches in drug discovery. Thus, the aim of this work was to evaluate the Passiflora mucronata Lam., Passifloraceae, potential for tuberculosis treatment. Specifically, related to antimycobacterial activity and anti-inflammatory related effects (based on inhibition of nitric oxide, tumor necrosis factor-alpha production and antioxidant potential), as well as the chemical profile of P. mucronata. High performance liquid chromatography coupled with diode-array ultraviolet and mass spectrometer analyses of crude hydroalcoholic extract and ethyl acetate fraction showed the presence of flavonoids. Ethyl acetate fraction showed to be as antioxidant as Ginkgo biloba standard extract with EC50 of 14.61 ± 1.25 µg/ml. One major flavonoid isolated from ethyl acetate fraction was characterized as isoorientin. The hexane fraction and its main isolated compound, the triterpene β-amyrin, exhibited significant growth inhibitory activity against Mycobacterium bovis BCG (MIC50 1.61 ± 1.43 and 3.93 ± 1.05 µg/ml, respectively). In addition, Passiflora mucronata samples, specially hexane and dichloromethane fractions, as well as pure β-amyrin, showed a dose-related inhibition of lipopolysaccharide (LPS)-induced nitric oxide production. In conclusion, Passiflora mucronata presented relevant biological potential and should be considered for further studies using in vivo pulmonary tuberculosis model.

RESUMO A ocorrência do oídio (Pseudoidium anacardii) está correlacionada com a brotação nova do cajueiro e à infecção que se processa em botões, flores e maturis jovens. Tecidos maduros tornam-se naturalmente resistentes ao fungo e, para o controle da doença, é necessária a proteção preventiva com fungicida à base de enxofre. Objetivou-se, com este trabalho, avaliar as épocas de floração e os períodos de proteção fenológica do cajueiro-anão BRS 189 à infecção de P. anacardii. O experimento foi realizado em blocos casualizados, com quatro repetições e em esquema de parcelas subdivididas, em que as parcelas foram duas épocas de floração e, as subparcelas, os seis períodos de proteção fenológica. Foram avaliados os graus de severidade do oídio na panícula, no pedúnculo e na castanha e a produção de cajus, durante as duas épocas do fluxo de florações. Verificou-se que as épocas e os períodos de proteção fenológica influenciaram no grau de severidade da doença e na produção de cajus. Para as variáveis de doença, observaram-se menores graus de severidade do oídio para os maiores períodos de proteção fenológica na panícula, no pedúnculo e na castanha. Não houve diferença significativa entre as épocas, quando se avaliaram os períodos de proteção fenológica, em relação ao peso total e pedúnculo, ao passo que, em ambas as épocas, o período de proteção (T6) foi diferente dos demais. Porém, não houve diferença entre épocas e período de proteção fenológica em relação ao peso das castanhas. Os resultados indicam que as épocas do fluxo de florações e os períodos de proteção fenológica interferem na severidade da doença e na produção de cajus do clone BRS 189.

ABSTRACT The occurrence of powdery mildew, Pseudoidium anacardii, is conditioned by new sprouting of the cashew tree and the infection that occurs in buds, flowers, and young maturis (green cashew nuts). Mature tissues naturally become resistant to fungus and, for the control of the disease, preventive protection with a sulfur-based fungicide is necessary. The objective was to verify the flowering time and phenological protection periods in the dwarf cashew BRS 189 against P. anacardii infection. The experimental design was randomized blocks in split-plot scheme with four replications. The main plots were flowering time (time I and II) and split plots were six phenological protection periods. The severity of powdery mildew in the panicle, peduncle, and cashew nuts and cashew production was evaluated. It was verified that the flowering times and the phenological protection periods influenced the severity of the disease and cashew production. For disease variables, lower severities of powdery mildew were observed for the greater phenological protection periods in panicle, peduncle, and chestnuts. There was no significant difference between the flowering times when the phenological protection periods were analyzed for total weight and peduncle. In both flowering times the protection period (T6) was different from the others. However, there was no difference between flowering times and phenological protection periods in relation to the weight of the nuts. The results indicate that flowering times and phenological protection periods interfere with the severity of the disease and cashew production (peduncle) of the BRS 189.

In Orchidaceae, association with symbiotic fungi is required for seed germination and seedling development, thereby being the main energy source during the first steps of germination. Colombia is one of the countries with the greatest biodiversity of orchids, with an estimated 3,200 species, but few studies on orchid mycorrhiza have been conducted. In our study, we isolated and sequenced the internal transcribed spacer rDNA region of fungi from two co-occurring Colombian epiphytic orchids, I. utricularioides and P. pusilla, both belonging to the subtribe Oncidiinae. All sequences were recognized as belonging to the genus Ceratobasidium, known to be a common orchid mycorrhizal fungus in both tropical and temperate orchids. One sequence was 100% similar to fungi isolated from I. utricularioides in Costa Rica in a previous study. I. utricularioides was confirmed to be a specialist, associating with only one clade of mycorrhizal fungi. However, P. pusilla was shown to be a generalist, associating with three clades. This finding indicates that the variation in mycorrhizal specificity could be an important factor in the co-existence of orchids. The high affinity between the subtribe Oncidiinae and Ceratobasidium was also reinforced.

Amblyomma rotundatum Koch é um carrapato partenogenético geralmente associado a répteis e anfíbios. Entretanto existem relativamente poucos estudos sobre a ocorrência de carrapatos em populações silvestres de répteis no Brasil. O objetivo deste estudo foi analisar a presença de carrapatos associados às espécies de répteis em uma comunidade na restinga de Grussaí, município de São João da Barra, Estado do Rio de Janeiro, Brasil. Foram amostradas, entre os meses de dezembro de 2010 e janeiro de 2011, 131 indivíduos pertencentes a nove espécies de répteis da ordem Squamata: lagartos Tropidurus torquatus (n = 51); Hemidactylus mabouia (n = 25), Mabuya agilis (n = 30), Mabuya macrorhyncha (n = 6), Cnemidophorus littoralis (n = 5) e Ameiva ameiva (n = 10), e serpentes Philodryas olfersii (n = 2), Oxyrhopus rhombifer (n = 1) e Micrurus corallinus (n = 1). A única espécie de carrapato encontrada associada a espécimes de répteis foi A. rotundatum, tendo sido encontrada uma fêmea adulta em um indivíduo do lagarto A. ameiva, uma ninfa em um T. torquatus e quatro ninfas em uma serpente P. olfersii. O presente estudo constitui o primeiro registro do parasitismo de A. rotundatum nos répteis T. torquatus e P. olfersii como hospedeiros. Nossos resultados sugerem que no habitat da restinga de Grussaí, A. rotundatum pode utilizar diferentes espécies de répteis para a realização do seu ciclo de vida.

Amblyomma rotundatum Koch is a parthenogenetic tick usually associated with reptiles and amphibians. However, relatively few studies on occurrences of ticks in wild reptile populations in Brazil have been produced. The aim of this study was to analyze the presence of ticks associated with reptile species in the Grussaí restinga, in the municipality of São João da Barra, state of Rio de Janeiro, Brazil. Between December 2010 and January 2011, 131 individuals belonging to nine species of reptiles of the order Squamata were sampled: the lizards Tropidurus torquatus (n = 51), Hemidactylus mabouia (n = 25), Mabuya agilis (n = 30), Mabuya macrorhyncha (n = 6), Cnemidophorus littoralis (n = 5) and Ameiva ameiva (n = 10); and the snakes Philodryas olfersii (n = 2), Oxyrhopus rhombifer (n = 1) and Micrurus corallinus (n = 1). The only tick species found to be associated with any of the reptiles sampled was A. rotundatum. One adult female was detected on one individual of the lizard A. ameiva, one nymph on one individual of the lizard T. torquatus and four nymphs on one individual of the snake P. olfersii. This study is the first record of parasitism of A. rotundatum involving the reptiles T. torquatus and P. olfersii as hosts. Our results suggest that in the Grussaí restinga habitat, A. rotundatum may use different species of reptiles to complete its life cycle.

A quantificação da divergência genética entre acessos de bancos de germoplasma baseada em descritores permite a indicação de potenciais genitores a serem utilizados em programas de melhoramento, entre outros resultados de interesse para o melhorista. Para estudos de divergência genética, a análise multivariada, incluindo os métodos de aglomeração, tem sido amplamente empregada. Este trabalho teve como objetivos caracterizar e quantificar a divergência genética entre acessos de tomateiro, por meio de métodos de agrupamento. O experimento foi conduzido em 2001, em Campos dos Goytacazes, RJ., em condições de campo. Utilizou-se o delineamento experimental de blocos casualizados com 70 acessos, três repetições e 16 plantas por parcela. Vinte descritores de caracterização e cinco de avaliação foram considerados. Houve diferença significativa entre os acessos para número total, peso total, número médio, e peso médio de frutos; comprimento e diâmetro do fruto; número de dias para germinação; número de dias para frutificação; número de flores por inflorescência; teor de sólidos solúveis; número de lóculos e número de dias para florescimento, indicando a presença de variabilidade entre os acessos. O agrupamento pelo Método Hierárquico do Vizinho Mais Próximo, detectou a formação de dois grupos, baseado no número de dias para germinação. O grupo 1 reuniu os acessos com germinação em 10 dias e o grupo 2 foi composto pelos acessos que germinaram em sete dias. O subagrupamento dos grupos 1 e 2 permitiu a detecção de sete subgrupos no grupo 1 e cinco subgrupos no grupo 2. O Método de Otimização de Tocher possibilitou a formação de dez grupos, concordantes com os subgrupos obtidos pelo método do Vizinho mais Próximo.

The quantification of the genetic divergence among accessions in a germplasm bank, based on descriptors for characterization, allows us to indicate promising parentals that can be used in breeding programs. In order to study the genetic divergence, multivariate analysis, including cluster methods has been used. The genetic divergence among tomato accessions was characterized and quantified, using cluster analysis. The experiment was carried out in 2001, in Rio de Janeiro State, Brazil, in field conditions. A randomized block design was used, with 70 accessions, three replications and 16 plants per plot. Twenty characterization and five evaluation descriptors were considered. There was significant difference among accessions for total number of fruits, total weight of fruits, mean number of fruits, mean weight of fruits, fruit length, fruit diameter, number of days for germination, days for fruit set, number of flowers per inflorescence, solid solubles, number of locules and days for flowering indicating the presence of genetic variability among accessions. Nearest neighbor method detected two groups, based on number of days for germination. Group 1 was formed by accessions with germination in 10 days while group 2 included accessions that germinated in seven days. Subgrouping from groups 1 and 2 detected seven and five subgroups for each group, respectively. Based on Tocher Method, ten groups were formed with agreement between Tocher and Nearest Neighbor.

Cinco acessos de feijão-de-vagem (UENF 1429, UENF 1432, UENF 1442, UENF 1445 e UENF 1448), e combinações híbridas F1, foram analisados quanto à capacidade geral e específica de combinação no delineamento em blocos ao acaso com quinze repetições. Decorrente da perda de parcelas utilizou-se o modelo de Griffing (1956) para dados desbalanceados, conforme proposto por Martins Filho et al. (1992). Os valores dos quadrados médios para capacidade geral de combinação foram altamente significativos, em 1% de probabilidade, pelo teste F, para todas as características avaliadas. Por sua vez, para capacidade específica de combinação as características número médio de sementes por vagem (NS) e altura média da inserção da primeira vagem (APV) não exibiram significância. Com base nos resultados de g i, os acessos UENF 1429, UENF 1442 e UENF 1445 são indicados como os mais promissores para uso 'per se'. Com relação aos híbridos, as combinações UENF 1429 x UENF 1432, UENF 1429 x UENF 1445, UENF 1432 x UENF 1445 e UENF 1445 x UENF 1448 têm potencial para geração de genótipos superiores.

Five accessions of snap bean (UENF 1429, UENF 1432, UENF 1442, UENF 1445 and UENF 1448) and their diallelic F1 hybrids were evaluated for general and specific combining ability in randomized complete block design with fifteen replications. Due to variation caused by the loss of plots, the Griffing's Methodology (1956) for unbalanced diallel was used, according to Martins Filho et al. (1992). Mean square values of the general combining ability were highly significant, at 1% of probability, for the F test, for all evaluated traits. For specific combining ability of mean number of seeds per pod and mean insertion of the first pod were not significant. Based on the g i results, the accessions UENF 1429, UENF 1442 and UENF 1445 were indicated for using 'per se'. The hybrids UENF 1429 x UENF 1432, UENF 1429 x UENF 1445, UENF 1432 x UENF 1445 and UENF 1445 x UENF 1448 have potential to obtain superior genotypes.