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RESUMO Dentre os ajustes fitotécnicos necessários, a correta recomendação da população de plantas de cada cultivar de soja é de suma importância para o sucesso na produção desta cultura. Assim, o objetivo deste estudo foi analisar a distribuição e autocorrelação espacial da população de plantas de soja de acordo com a variabilidade espacial de atributos físicos e químicos de um Latossolo na região do Cerrado brasileiro. Os atributos estudados foram: população de plantas, produtividade de grãos de soja, condutividade elétrica aparente do solo, teor de matéria orgânica do solo, capacidade de troca catiônica, potencial hidrogeniônico e teor de argila no solo. A população de plantas variou inversamente em função da condutividade elétrica aparente do solo. As áreas com os maiores valores de condutividade elétrica aparente do solo, teor de matéria orgânica do solo, capacidade de troca catiônica e teor de argila apresentaram as menores populações de plantas. A produtividade da soja não foi influenciada pelo aumento ou diminuição da população de plantas, por isso é economicamente viável plantar com menor densidade de plantas para melhorar a margem de lucro do agricultor. necessários cultura Assim brasileiro foram solo agricultor

ABSTRACT Among the necessary phytotechnical adjustments, the correct recommendation of the plant population of each soybean cultivar is of paramount importance for success in crop production. Thus, the objective of this study was to analyze the distribution and spatial autocorrelation of the soybean plant population according to the spatial variability of the physical and chemical attributes of Oxisol in the Brazilian Cerrado region. The attributes studied were plant population, soybean grain yield, apparent soil electrical conductivity, soil organic matter content, cation exchange capacity, hydrogenionic potential, and clay content in the soil. The plant population varied inversely as a function of apparent soil electrical conductivity. The areas with the highest values of apparent soil electrical conductivity, soil organic matter content, cation exchange capacity, and clay content had the lowest plant populations. Soybean yield was not influenced by increase or decrease in the plant population. Thus, it is economically viable to plant with lower plant density to improve the farmer’s profit margin. adjustments production Thus region conductivity capacity potential populations farmers farmer s margin

https://doi.org/10.1590/1807-1929/agriambi.v28n1e264872

Pesticide Determination in Fresh Coconut Water (Cocos nucifera Linn.) by GC-MS Using Microwave-Assisted Liquid-Liquid Extraction
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Nakamura, Thamilin C.

; Rego, Enoc L. do

; Souza, Jurandir R. de

; Diniz, Paulo H. G. D.

; Silva, José D. S. da

Journal of the Brazilian Chemical Society

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nov 2023, Volume 34 N. 11 Pages 1707 - 1716

Abstract: En | Text: En | PDF: En

Fresh coconut (Cocos nucifera) water, a popular beverage in tropical countries, is often exposed to pesticide contamination due to agricultural practices. Thus, this work proposed the simultaneous determination of 36 pesticides in fresh coconut water samples using liquid-liquid extraction, drying assisted by a household microwave oven, and gas chromatography-mass spectrometry (GC-MS) analysis. Limits of detection varied from 5.4 (disulfoton) to 9.6 ng L-1 (parathion-methyl). Nine pesticide residues were detected (dimethoate, γ-HCH, β-HCH, 4,4’-dichlorodiphenyldichloroethylene, endosulfan II, 4,4’-dichlorodiphenyldichloroethane, 4,4’-dichlorodiphenyltrichloroethane, endrin ketone, and methoxychlor) with concentrations from < limit of quantification to 5,768.34 ± 1.64 ng L-1. Dimethoate was quantified in all samples, indicating its recent use. The proposed methodology offers several advantages, including the use of small sample volumes and solvent extraction, which eliminates the need for costly cartridges and reduces waste production. Additionally, the obtained results can be valuable for regulatory agencies, aiding in the mitigation of environmental damage and the protection of human health.

https://doi.org/10.21577/0103-5053.20230125

Fertilization with zinc improves the growth and photosynthetic performance of dwarf green coconut seedlings
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Moraes, Ana R. A. de

; Araújo, Suzana R.

; Silva Junior, Mário L. da

; Lins, Paulo M. P.

; Gomes, Maynara S.

; Gomes, Mila F.

; Marques, Jonathan D.

; Dias, Victor H. R.

Revista Brasileira de Engenharia Agrícola e Ambiental

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nov 2023, Volume 27 N. 11 Pages 864 - 872

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT Zinc is an essential micronutrient for plant metabolism and has been studied in several crops of economic interest. However, there is no evidence of its use in coconut crops. The objective of this study was to evaluate the influence of foliar zinc fertilization with doses of zinc on dwarf green coconut seedlings based on their nutritional status, growth attributes, and gas exchange. The experimental design consisted of randomized blocks, and the treatments consisted of different zinc doses (0, 5, 10, 15, 20, and 25 g per plant) with five replicates. Seedlings were positively influenced by zinc dose, mainly in terms of growth variables and gas exchange. Zinc fertilization reduced iron and manganese content and increased zinc concentrations in the leaf tissue. Zinc fertilization promoted an increase in leaf area, height, shoot dry mass, and total dry mass. Furthermore, zinc fertilization improved photosynthesis, transpiration, stomatal conductance, internal CO2 concentration, instantaneous water use efficiency, and intrinsic water use efficiency. The improvement in these growth indices and gas exchange, in most variables, was up to an estimated dose of approximately 16 g of zinc per plant and decreased with increasing doses.

RESUMO O zinco é um micronutriente de importância essencial para o metabolismo vegetal, é estudado para diversas culturas de interesse econômico, porém sem evidências de estudo para a cultura do coqueiro. Objetivou-se com este estudo avaliar a influência da fertilização com doses de zinco via foliar em mudas de coqueiro anão verde, por meio do estado nutricional, atributos de crescimento e trocas gasosas. O delineamento experimental foi em blocos ao acaso, e os tratamentos foram constituídos por doses de zinco (0, 5, 10, 15, 20 e 25 g por planta), com cinco repetições. As mudas foram influenciadas positivamente pelas doses de zinco principalmente nas variáveis de crescimento e nas trocas gasosas. No estado nutricional afetou de maneira decrescente o ferro e o manganês e aumentou o zinco no tecido foliar. O zinco proporcionou incrementos na área foliar, altura de plantas, massa seca da parte aérea e massa seca total. Além disso, melhorou a fotossíntese, transpiração, condutância estomática, concentração interna de CO2, eficiência instantânea do uso da água e eficiência intrínseca do uso da água. A melhora nesses índices de crescimento e trocas gasosas, na maioria das variáveis, foi até a dose estimada de aproximadamente 16 g de zinco por planta e diminuiu com o aumento das doses.

https://doi.org/10.1590/1807-1929/agriambi.v27n11p864-872

Long-term oncological and surgical outcomes after Video Endoscopic Inguinal Lymphadenectomy (VEIL) in patients with penile cancer
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Tobias-Machado, Marcos

; Ornellas, Antonio A.

; Hidaka, Alexandre K.

; Medina, Luis G.

; Mattos, Pablo A. L.

; Besio, Ruben S.

; Abreu, Diego

; Castro, Pedro R.

; Nishimoto, Ricardo H.

; Astigueta, Juan

; Dourado, Aurus

; Machado, Roberto D.

; Magnabosco, Wesley J.

; Corona-Montes, Victor

; Villoldo, Gustavo M.

; Zampolli, Hamilton C.

; Taha, Anis

; Auad, Pericles R.

; Faria, Eliney F.

; Arantes, Paulo B. O.

; Tavares, Alessandro

; Nascimento, Francisco S. M. S.

; Brazão Jr., Eder S.

; Rocha, Maurício M.

; Costa, Walter H.

; Panico, Vinicius

; Reis, Leonardo O.

; Almeida-Carrera, Roberto J.

; Silva, Rafael C.

; Zequi, Stênio C.

; Calixto, José R. R.

; Sotelo, Rene

International braz j urol

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oct 2023, Volume 49 N. 5 Pages 580 - 589

Abstract: En | Text: En | PDF: En

ABSTRACT Objective: To report outcomes from the largest multicenter series of penile cancer patients undergoing video endoscopic inguinal lymphadenectomy (VEIL). Materials and Methods: Retrospective multicenter analysis. Authors of 21 centers from the Penile Cancer Collaborative Coalition-Latin America (PeC-LA) were included. All centers performed the procedure following the same previously described standardized technique. Inclusion criteria included penile cancer patients with no palpable lymph nodes and intermediate/high-risk disease and those with non-fixed palpable lymph nodes less than 4 cm in diameter. Categorical variables are shown as percentages and frequencies whereas continuous variables as mean and range. Results: From 2006 to 2020, 210 VEIL procedures were performed in 105 patients. Mean age was 58 (45-68) years old. Mean operative time was 90 minutes (60-120). Mean lymph node yield was 10 nodes (6-16). Complication rate was 15.7%, including severe complications in 1.9% of procedures. Lymphatic and skin complications were noted in 8.6 and 4.8% of patients, respectively. Histopathological analysis revealed lymph node involvement in 26.7% of patients with non-palpable nodes. Inguinal recurrence was observed in 2.8% of patients. 10y- overall survival was 74.2% and 10-y cancer specific survival was 84.8%. CSS for pN0, pN1, pN2 and pN3 were 100%, 82.4%, 72.7% and 9.1%, respectively. Conclusion: VEIL seems to offer appropriate long term oncological control with minimal morbidity. In the absence of non-invasive stratification measures such as dynamic sentinel node biopsy, VEIL emerged as the alternative for the management of non-bulky lymph nodes in penile cancer.

https://doi.org/10.1590/s1677-5538.ibju.2023.0065

Production of pear cv. ‘Triunfo’ under irrigation systems and depths in the sub-middle São Francisco region
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Gomes, Victor H. F.

; Simões, Welson L.

; Garrido, Marlon da S.

; Lopes, Paulo R. C.

; Silva, Jucicléia S. da

Revista Caatinga

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sep 2023, Volume 36 N. 3 Pages 620 - 629

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT Aiming at the search for a new alternative for the sustainability of the properties in the irrigated areas of the Brazilian Northeast, the objective of this study was to identify the ideal irrigation system and depth for a better yield and quality of pear fruits in the Sub-middle São Francisco region. The experiment was conducted in the commercial orchard of the Frutos do Sol farm, located in the municipality of Petrolina, PE, Brazil, from October 2016 to November 2017. The treatments consisted of four irrigation depths (60; 80; 100 and 120% of crop evapotranspiration - ETc), under two irrigation systems (drip and micro-sprinkler), in two crop cycles of ‘Triunfo’ pear. Yield, average fruit weight, water productivity, pulp firmness and titratable acidity, total soluble solids and lightness of the fruits were analyzed. Irrigation depths of 96.04% and 94.84% ETc promote, respectively, the highest marketable yields for the first (43.48 t ha-1) and second (26.55 t ha-1) cycle of ‘Triunfo’ pear in the Brazilian semi-arid region. Drip irrigation system in the warmer period promotes higher yield. The cultivation of ‘Triunfo’ pear irrigated by drip and micro-sprinkler promotes fruits with good commercial quality in the Brazilian semi-arid region.

RESUMO Visando a busca de uma nova alternativa para sustentabilidade das propriedades dos polos irrigados do Nordeste brasileiro, objetivou-se com este estudo identificar o sistema e a lâmina de irrigação ideal para uma melhor produtividade e qualidade dos frutos da pereira, no Submédio São Francisco. O experimento foi conduzido no pomar comercial da fazenda Frutos do Sol, localizado no município de Petrolina - PE, no período de outubro de 2016 a novembro de 2017. Os tratamentos foram constituídos de quatro lâminas de irrigação (60; 80; 100 e 120% da evapotranspiração da cultura - ETc), sob dois sistemas de irrigação (gotejamento e microaspersão), em dois ciclos de cultivos da pereira ‘Triunfo’. Analisou-se a produtividade, peso médio dos frutos, produtividade de água, bem como a firmeza de polpa e a acidez titulável, sólidos solúveis totais e luminosidade dos frutos. As lâminas de irrigação de 96,04% e 94,84% da ETc proporcionam, respectivamente, as maiores produtividades comerciais para o primeiro (43,48 t ha-1) e segundo (26,55 t ha-1) ciclo de cultivo da pereira ‘Triunfo’ no Semiárido brasileiro. O sistema de irrigação por gotejamento no período mais quente proporciona maior produtividade. O cultivo da pereira ‘Triunfo’ irrigada por gotejamento e microaspersão proporcionam frutos com boa qualidade comercial no Semiárido brasileiro.

https://doi.org/10.1590/1983-21252023v36n315rc

Production, gas and biochemical exchanges in pear cultivated in semi-arid region under different irrigation managements
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Gomes, Victor H. F.

; Simões, Welson L.

; Silva, Jucicléia S. da

; Garrido, Marlon da S.

; Silva, José A. B. da

; Lopes, Paulo R. C.

; Silva, Weslley O. da

; Santos, Leane R. dos

Revista Brasileira de Engenharia Agrícola e Ambiental

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may 2023, Volume 27 N. 5 Pages 335 - 342

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

ABSTRACT The objective of this study was to evaluate the influence of irrigation systems and water depths on physiological, biochemical and production processes of pear trees grown in the Brazilian semi-arid region. The experimental design was randomized blocks, with 2 × 4 factorial scheme, corresponding to two irrigation systems (drip and microsprinkler) and four irrigation depths (60, 80, 100 and 120% of the crop evapotranspiration - ETc), with four replicates. Water deficit and excess applied to plants are harmful to gas exchange as well as to biosynthesis and accumulation of carbohydrates, amino acids and proteins in leaves, compromising the cultivation cycle of pear plants grown in the Sub-Middle São Francisco region. The irrigation depth of 91.8% ETc promotes the highest production of pear (18.49 kg plant-1) under drip and microsprinkler irrigation systems, under the edaphoclimatic conditions of the Sub-Middle São Francisco region.

RESUMO O objetivo deste trabalho foi avaliar a influência de sistemas de irrigação e lâminas de irrigação sobre processos fisiológicos, bioquímicos e produtivos da pereira cultivada no Semiárido brasileiro. O delineamento experimental foi em blocos casualizados, com fatorial 2 x 4, sendo dois sistemas de irrigação (gotejamento e microaspersão) e quatro lâminas de irrigação (60; 80; 100 e 120% da evapotranspiração da cultura - ETc), com quatro repetições. A deficiência e o excesso hídrico aplicados às plantas são prejudiciais às trocas gasosas, bem como à biossíntese e acúmulo de carboidratos, aminoácidos e proteínas nas folhas, comprometendo o ciclo de cultivo de pereiras cultivadas na região do Submédio São Francisco. A lâmina de irrigação de 91,8% ETc promove a maior produção de pereira (18,49 kg planta-1) sob sistemas de irrigação por gotejamento e microaspersão, nas condições edafoclimáticas do Submédio São Francisco.

https://doi.org/10.1590/1807-1929/agriambi.v27n5p335-342

Multidisciplinary Scientific Cruises for Environmental Characterization in the Santos Basin – Methods and Sampling Design
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Moreira, Daniel L.

; Dalto, Adriana G.

; Figueiredo JR, Alberto G.

; Valerio, Aline M.

; Detoni, Amalia M. S.

; Bonecker, Ana C. T.

; Signori, Camila N.

; Namiki, Cláudia

; Sasaki, Dalton K.

; Pupo, Daniel V.

; Silva, Danilo A.

; Kutner, Deborah S.

; Duque-Castaño, Diana C.

; Marcon, Eduardo H.

; Gallotta, Fabiana D. C.

; Paula, Fabiana S.

; Gallucci, Fabiane

; Roque, Gabriela C. F.

; Campos, Giulia S.

; Fonseca, Gustavo

; Mattos, Gustavo

; Lavrado, Helena P.

; Silveira, Ilson C. A. da

; Costa, Jessica O.

; Santos Filho, João R. dos

; Carneiro, Juliane C.

; Moreira, Julio C.F.

; Rozo, Laura

; Araujo, Leandro F.M.

; Lazzari, Letícia

; Silva, Letícia O. da

; Michelazzo, Luan S.

; Fernandes, Luciano F.

; Dottori, Marcelo

; Araújo Jr., Marcus A. G. de

; Chuqui, Mateus G.

; Ceccopieri, Milena

; Borges-Silva, Milton

; Kampel, Milton

; Bergo, Natascha M.

; Silva, Paulo V. M.

; Tura, Pedro M.

; Moura, Rafael B. de

; Romano, Renato G.

; Martins, Renato P.

; Carreira, Renato S.

; Toledo, Rodrigo G.A.

; Bonecker, Sérgio L.C.

; Disaró, Sibelle T.

; Rodrigues, Silvana V.

; Corbisier, Thais N.

; Vicente, Thaisa M.

; Paiva, Vitor G. de

; Pellizari, Vivian H.

; Belo, Wellington C.

; Brandini, Frederico P.

; Sousa, Silvia H.M

Ocean and Coastal Research

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2023, Volume 71 Suppl. 3 elocation e23022

Abstract: En | Text: En | PDF: En

Abstract The Santos Basin (SB) is the main petroliferous basin in the Brazilian continental margin and one of the most studied marine areas in Brazil. However, historical data suggest that new efforts should be carried out to acquire quantitative biological data, especially in the deep sea, to establish the baseline of essential ocean variables in different ecosystems for future monitoring programs. The Brazilian energy company Petrobras planned and executed 24 oceanographic cruises over a period of 2 years to assess the benthic (SANSED cruise) and pelagic (SANAGU cruise) systems of the SB (356 days at sea in 2019 and 2021/2022). These efforts were part of the Santos Project, which comprised a comprehensive environmental study aimed at investigating benthic and pelagic variables to characterize ecology, biogeochemistry, thermohaline properties of water masses, and ocean circulation patterns, geomorphology, and sedimentology, as well as organic and inorganic chemistry. Here we present the detailed sampling designs and the field methods employed on board, during the SB scientific cruises. All sampling protocols were based on standardized approaches. For the benthos analyses, triplicate sediment samples were performed using a GOMEX-type box corer (0.25 m²) or a large modified Van Veen grab (0.75 m²) at 100 stations ranging from 25 to 2400 m depth. At each station, 25 geochemical and physico-chemical parameters were analyzed in addition to micro-, meio-, and macrofauna and living foraminifera samples. For the pelagic system, 60 stations were selected to investigate the plankton community, ranging in size from pico- to macroplankton, through vertical, horizontal, and oblique net hauls (20, 200, and 500 μm mesh size), as well as 25 biogeochemical parameters collected with an aid of a CTD-rosette sampler. Part of this scientific information also serves the Regional Environmental Characterization Project (PCR-BS) in support of Petrobras’ Santos Basin drilling licensing process led by the Brazilian Environmental Agency – IBAMA. This project contributes to the sustainable development of the SB, in line with the guidelines of the United Nations Decade of Ocean Science for Sustainable Development.

https://doi.org/10.1590/2675-2824071.22072dlm

Simulation of the electrical percolation of carbonnano tube composites in polymethyl methacrylatematrix: two-dimensional andthree-dimensional analysesusing a tortuosityeffectofnanofillers
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Silva, Jessica P

; Sousa, Lailson

; Vélez, Harvey A Villa

; Coelho, Paulo H. S. L.

Revista de Ciencia y Tecnología

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jun 2022, N. 37 Pages 71 - 80

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Resumen El presente trabajotiene como objetivo estudiar la simulación de la percolación eléctrica de nanotubos de carbono (CNT) en matriz de polimetilmetacrilato (PMMA). Para ello, se utilizaron simulaciones en análisis bidimensionales y tridimensionales a través de un programa desarrollado utilizando el método Monte Carlo, apoyado en el modelo de volumen excluido para analizar la tortuosidad geométrica en la matriz polimérica. El umbral de percolación se analizó mediante diferentes relaciones de aspecto, fracciones volumétricas y geometrías de carga conductiva. En las simulaciones, se observó una disminución en el umbral de percolación cuando aumentaba la relación de aspecto y las partículas se volvían más tortuosas, siendo estas características los parámetros para evaluar sistemas tanto bidimensionales como tridimensionales. Los resultados muestran valores de umbral de percolación de 0.625% vol y relación de aspecto de 1000 para análisisbidimensionales y 0.08% vol y relación de aspecto de 250 para análisis tridimensionales, siendo la adecuación a los principales modelos de percolación eléctrica conefecto de tortuosidadlosnanoaditivospropuestos por la literatura.

Abstract The present work aims to study the simulation of the electrical percolation of carbon nanotubes (CNTs) in polymethylmethacrylate matrix (PMMA). In order to do this, simulations were employed in two-dimensional and three-dimensional analyses through a program developed by the Monte Carlo method, supported by the excluded volume model in order to analyze the geometric tortuosity in the polymeric matrix. The percolation threshold was analyzed by different aspect ratios, volumetric fractions and conductive load geometries. In the simulations, a decrease in the percolation threshold was observed when the aspect ratio increased and the particles became more tortuous, being these characteristics the parameters to evaluate both two-dimensional and three-dimensional systems. The results show percolation threshold values of 0.625% vol and aspect ratio of 1000 for two-dimensional analyses and, 0.08% vol and aspect ratio of 250 for three-dimensional analyses, being the adequacy to the main models for electrical percolation with tortuosity effect of the nanocarriers proposed by literature.

https://doi.org/10.36995/j.recyt.2022.37.008 16 downloads

AN ADAPTED UNMANNED AERIAL VEHICLE FOR ENVIRONMENTAL WATER SAMPLING
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D. Neto, José C.

; Resque, Ian S.

; Avelino, Rodrigo A.

; Santos, Vagner B. dos

; Leite, Lucas S.

; Cesar, Lucas O.

; Belo, Paulo H. da Silva

; Albuquerque, Jones

; Lima Filho, José L. de

Química Nova

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jun 2022, Volume 45 N. 6 Pages 734 - 741

Abstract: En | Text: En | PDF: En

In this work an unmanned aerial vehicle (UAV) was adapted to be used as an environmental tool to collect water samples of sea, estuary, and mangrove for further analysis by gas chromatography with a mass detector (GC-MS). The sampling automated system was based on a microcontroller, a solenoid valve, and a peristaltic mini-pump that was coupled to a quadcopter UAV. The apparatus was properly calibrated to acquire 100 mL of environmental water at 4.6 min. After, the concentration of BTEX (benzene, toluene, ethylbenzene, and o, m, p-xylenes) was analyzed in laboratory using GC-MS and the BTEX concentration ranged from 0.3 to 5.0 µg L-1 with a recovery from 83.9 to 118% with a relative standard deviation lower than 7.0%. Tests of cross-contamination were performed by simultaneous sampling and laboratory blank was carried out and no contamination was found for BTEX analysis. Based on the results, the adapted UAV presented a very useful ability to collect samples in difficult access areas such as mangroves, points between rocks, corals reefs, without health risk and without impacting the fragile ecosystem being an interesting tool for environmental purposes.

https://doi.org/10.21577/0100-4042.20170892

10.

A QUÍMICA POR TRÁS DOS MEDICAMENTOS DISTRIBUÍDOS PELO PROGRAMA FARMÁCIA POPULAR NO BRASIL: ROTAS SINTÉTICAS, RELAÇÃO ESTRUTURA-ATIVIDADE E PERSPECTIVAS FUTURAS
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Santos, Giovanny C. dos

; Rodrigues, Julia L.

; Souza, Júllia R. de

; Silva-Filho, Luiz C. da

; Silva, Bruno H. S. T. da

Química Nova

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2021, Volume 44 N. 10 Pages 1280 - 1299

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Certainly, most chemistry students, patients or simply curious, have already questioned themselves about certain medications present in their daily lives, trying to understand how they work and even how they can be obtained. Based on that, this work presents some known synthetic routes and the structure-activity relationship (SAR), of some active pharmaceutical ingredients present in medications distributed in Brazil by the government program known as “Farmacia Popular”, emphasizing the functional groups and chemical structure linked to their mechanisms of action. Some other characteristics and future perspectives of such drugs and their respective active principles are also highlighted.

https://doi.org/10.21577/0100-4042.20170770

11.

COVID-19: The question of genetic diversity and therapeutic intervention approaches
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Figueiredo, David Livingstone Alves

; Ximenez, João Paulo Bianchi

; Seiva, Fábio Rodrigues Ferreira

; Panis, Carolina

; Bezerra, Rafael dos Santos

; Ferrasa, Adriano

; Cecchini, Alessandra Lourenço

; Medeiros, Alexandra Ivo de

; Almeida, Ana Marisa Fusco

; Ramão, Anelisa

; Boldt, Angelica Beate Winter

; Moya, Carla Fredrichsen

; Chin, Chung Man

; Paula, Daniel de

; Rech, Daniel

; Gradia, Daniela Fiori

; Malheiros, Danielle

; Venturini, Danielle

; Tavares, Eliandro Reis

; Carraro, Emerson

; Ribeiro, Enilze Maria de Souza Fonseca

; Pereira, Evani Marques

; Tuon, Felipe Francisco

; Follador, Franciele Aní Caovilla

; Fernandes, Glaura Scantamburlo Alves

; Volpato, Hélito

; Cólus, Ilce Mara de Syllos

; Oliveira, Jaqueline Carvalho de

; Rodrigues, Jean Henrique da Silva

; Santos, Jean Leandro dos

; Visentainer, Jeane Eliete Laguila

; Brandi, Juliana Cristina

; Serpeloni, Juliana Mara

; Bonini, Juliana Sartori

; Oliveira, Karen Brajão de

; Fiorentin, Karine

; Lucio, Léia Carolina

; Faccin-Galhardi, Ligia Carla

; Ferreto, Lirane Elize Defante

; Lioni, Lucy Megumi Yamauchi

; Consolaro, Marcia Edilaine Lopes

; Vicari, Marcelo Ricardo

; Arbex, Marcos Abdo

; Pileggi, Marcos

; Watanabe, Maria Angelica Ehara

; Costa, Maria Antônia Ramos

; Giannini, Maria José S. Mendes

; Amarante, Marla Karine

; Khalil, Najeh Maissar

; Lima Neto, Quirino Alves de

; Herai, Roberto H.

; Guembarovski, Roberta Losi

; Shinsato, Rogério N.

; Mainardes, Rubiana Mara

; Giuliatti, Silvana

; Yamada-Ogatta, Sueli Fumie

; Gerber, Viviane Knuppel de Quadros

; Pavanelli, Wander Rogério

; Silva, Weber Claudio da

; Petzl-Erler, Maria Luiza

; Valente, Valeria

; Soares, Christiane Pienna

; Cavalli, Luciane Regina

; Silva Jr, Wilson Araujo

Genetics and Molecular Biology

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2021, Volume 44 N. 1 elocation e20200452

Abstract: En | Text: En | PDF: En

Abstract Coronavirus disease 2019 (COVID-19), caused by the Severe Acute Respiratory Syndrome Coronavirus type 2 (SARS-CoV-2), is the largest pandemic in modern history with very high infection rates and considerable mortality. The disease, which emerged in China’s Wuhan province, had its first reported case on December 29, 2019, and spread rapidly worldwide. On March 11, 2020, the World Health Organization (WHO) declared the COVID-19 outbreak a pandemic and global health emergency. Since the outbreak, efforts to develop COVID-19 vaccines, engineer new drugs, and evaluate existing ones for drug repurposing have been intensively undertaken to find ways to control this pandemic. COVID-19 therapeutic strategies aim to impair molecular pathways involved in the virus entrance and replication or interfere in the patients’ overreaction and immunopathology. Moreover, nanotechnology could be an approach to boost the activity of new drugs. Several COVID-19 vaccine candidates have received emergency-use or full authorization in one or more countries, and others are being developed and tested. This review assesses the different strategies currently proposed to control COVID-19 and the issues or limitations imposed on some approaches by the human and viral genetic variability.

https://doi.org/10.1590/1678-4685-gmb-2020-0452

12.

Evaluation of the Synthesis of Zeolite SSZ-13 using Choline Chloride and Different Crystallization Seeds and its Application in the Thermocatalytic Degradation of Ultra-High Molecular Weight Polyethylene
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Solano, Julyane R. S.

; Nascimento, Raul C. S.

; Silva, David C. M.

; Silva, Diogo P. S.

; Sousa, Lenivaldo V.

; Silva, Bruno J. B.

; Alencar, Soraya L.

; Urbina, Maritza M.

; Quintela, Paulo H. L.

; Silva, Antonio O. S.

Materials Research

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2021, Volume 24 N. 5 elocation e20210066

Abstract: En | Text: En | PDF: En

Abstract The synthesis of SSZ-13 was studied by evaluating the influence of two types of crystallization seeds with CHA structure (S1 seeds prepared in fluoride medium and S2 seeds by the conversion of zeolite Y), SiO2/Al2O3 (28, 40, 60 and 100) and OH/SiO2 (0.6 and 0.8) ratios. Choline chloride was used as the structure-directing agent and the crystallizations occurred at 140 ºC from 24 to 96 h. S1 seeds led to the coexistence of zeolites SSZ-13 and P, while S2 seeds promoted in all SiO2/Al2O3 ratios and OH/SiO2 = 0.8, the formation of pure SSZ-13 after 24 h. The SSZ-13 samples are formed by clusters of crystals with cuboid-like morphology and exhibited textural properties typical of CHA framework. SSZ-13 zeolite led to a decrease in the temperature and the activation energy required for the degradation of ultra-high molecular weight polyethylene polymer, demonstrating its efficiency as catalyst in this process.

https://doi.org/10.1590/1980-5373-mr-2021-0066 152 downloads

13.

ANTIPROMASTIGOTE AND CYTOTOXIC ACTIVITIES OF FLAVONOIDS FROM Fridericia speciosa LEAVES
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Milani, Reinaldo R.

; Alves, Osvaine J. A.

; Bertanha, Camila S.

; Gimenez, Valéria M. M.

; Squarisi, Iara S.

; Nicolella, Heloiza D.

; Pagotti, Mariana C.

; Tavares, Denise C.

; Silva, Márcio L. A. e

; Cunha, Wilson R.

; Magalhães, Lizandra G.

; Januario, Ana H.

; Pauletti, Patricia M.

Química Nova

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dec 2020, Volume 43 N. 10 Pages 1404 - 1407

Abstract: En | Text: En | PDF: En

Fridericia speciosa (Bignoniaceae) is a liana that occurs in humid to dry vegetation in eastern and southeastern Brazil. Species belonging to the genus Fridericia are employed as medicinal plants. This study evaluates the antipromastigote and cytotoxic activities of the crude extract, fractions, and compounds isolated from F. speciosa, and an HPLC (High-Performance Liquid Chromatography) analytical method was used to quantify the bioactive flavonoids. The flavonoids alpinetin (1), luteolin (2), diosmetin (3), and apigenin (4) were isolated from the active EtOAc-soluble fraction (EAF) of the crude ethanol extract (LCE) obtained from F. speciosa leaves. Both LCE and EAF were active against the normal human lung fibroblast cell line GM07492A (IC50 of 460.7 µg mL-1 and 381.4 µg mL-1, respectively). Only EAF showed activity against Leishmania amazonensis (80.4% at 50 µg mL-1). Compounds 1 and 2 were quantified by HPLC-DAD in both LCE (1: 6.21%; 2: 1.11%) and EAF (1: 45.32%; 2: 2.00%). At 100 µM, compounds1-4 inhibited L. amazonensis flagellar motility by 40.2%, 26.8%, 1.0%, and 24.8%, respectively, while their toxicities (IC50) to the normal cell were > 1000, 353.0, 766.2, and >1000 µg mL-1, respectively. Therefore, F. speciosa leaves are a prolific source of bioactive flavonoids.

https://doi.org/10.21577/0100-4042.20170628 159 downloads

14.

Effect of pre-anesthetic fasting on gastric emptying and plasma glucose in healthy dogs of different age groups
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Lecheta, Danielle R.

; Silva, Dheywid K.M.

; Santos, Guilherme A.

; Cunha, Marcelo S.

; Gaspar, Tamiris T.

; Lopes, Bruno A.

; Deboleto, Sandriele G.C.

; Braz, Paulo H.

Pesquisa Veterinária Brasileira

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apr 2020, Volume 40 N. 4 Pages 289 - 292

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: Avaliou-se o esvaziamento gástrico e a glicemia plasmática em cães jovens e adultos, alimentados com ração seca e úmida, submetidos a diferentes períodos de jejum pré-anestésico (6, 8 e 12 horas). Foram selecionados 40 cães hígidos, os quais foram segmentados em 4 grupos de acordo com a faixa etária e o tipo de dieta administrada. Foi avaliado o esvaziamento gástrico por ultrassonografia e a glicemia sérica. Apenas 17,5% apresentaram completo esvaziamento gástrico, não sendo encontradas diferenças significativas entre as avaliações com 6 e 8 horas de jejum, ou entre as faixas etárias e dietas, considerando nível de significância p<0,05. Os valores médios da glicose plasmática dos grupos indicaram normoglicemia em todos os momentos de avaliação. Foi encontrada diferença significativa entre as médias da glicemia dos cães jovens e adultos, no período de 8 horas de jejum com dieta úmida (p=0,03) e com 12 horas nos animais com dieta seca (p=0,04). Conclui-se que cães hígidos jovens e adultos, em equilíbrio fisiológico, mantêm valores normais de glicemia plasmática apesar de períodos prolongados de jejum pré-anestésico, os quais podem ser necessários, tendo em vista que 8 horas de jejum alimentar de sólidos não é suficiente para proporcionar completo esvaziamento gástrico.

ABSTRACT: Gastric emptying and plasma glucose were evaluated in young and adult dogs, fed with dry and wet food, submitted to different periods of pre-anesthetic fasting (6, 8, and 12 hours). Forty healthy dogs were selected, which were segmented into four groups according to the age group and type of diet. It was evaluated the gastric emptying by ultrasound and serum glycemia. Only 17.5% presented complete gastric emptying, and no significant differences were found between the 6 and 8-hour fasting evaluations, or between the age groups and the diets, considering significance level p<0.05. Mean plasma glucose values from the groups indicated normal glycemia at all times of evaluation. A significant difference was found between the means of glycemia in young and adult dogs, with the 8-hour fasting with wet diet (p=0.03) and with 12 hours with dry diet (p=0.04). Healthy young and adult dogs, in physiological equilibrium, maintain average values of plasma glucose despite prolonged periods of pre-anesthetic fasting, which may be necessary, since 8-hour fasting for solid food is not enough to provide complete gastric emptying.

https://doi.org/10.1590/1678-5150-pvb-6260 889 downloads

15.

Hancornia speciosa serum fraction latex stimulates the angiogenesis and extracellular matrix remodeling processes
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D’ABADIA, PATRÍCIA L.

; BAILÃO, ELISA FLÁVIA LUIZ C.

; LINO JÚNIOR, RUY S.

; OLIVEIRA, MATHEUS GABRIEL

; SILVA, VINICIUS B.

; OLIVEIRA, LEANDRA A.R.

; CONCEIÇÃO, EDEMILSON C.

; MELO-REIS, PAULO ROBERTO

; BORGES, LEONARDO LUIZ

; GONÇALVES, PABLO JOSÉ

; ALMEIDA, LUCIANE M.

Anais da Academia Brasileira de Ciências

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2020, Volume 92 N. 2 elocation e20190107

Abstract: En | Text: En | PDF: En

Abstract The Hancornia speciosa latex reveals angiogenic, osteogenic, and anti-inflammatory properties, which present its potential for developing of wound healing drugs; however, the latex compounds responsible for angiogenesis remain unknown. One strategy to screen these active compounds is evaluation of latex fractions. This study aimed to obtain different fractions of latex and evaluate its angiogenic activity separately using the chick chorioallantoic membrane (CAM) assay. The serum (SE) fraction was responsible for angiogenesis, which was subject to biochemical characterization and computational simulations in order to understand the contribution of H. speciosa latex in wound healing process. Our results revealed weak antioxidant potential and absence of antimicrobial activity in the SE fraction. Phytochemical analysis identified chlorogenic acids (CGA) as the main compound of SE fraction. CGA bioactivity predictions identify different molecules associated with extracellular matrix (ECM) remodeling, such as metalloproteinases, which also are overexpressed in our CAM assay experiment. Docking simulations revealed the interactions between CGA and matrix metalloproteinase 2. In conclusion, SE latex fraction stimulates angiogenesis and may influence ECM remodeling. These properties may contribute to the wound healing process, and also confirm the widespread use of this plant.

https://doi.org/10.1590/0001-3765202020190107 1041 downloads

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