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au:SILVA, TATIANA L DA

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Maize yield in an integrated crop-livestock-forestry system in south Goiás, Brazil
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Silva, Flávia Feliciano da Costa

; Ferreira, Jorge Luís Sousa

; Ramos, Tatiana Vieira

; Calil, Francine Neves

Revista Ceres

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jun 2020, Volume 67 N. 3 Pages 176 - 180

Abstract: En | Text: En | PDF: En

ABSTRACT Cerrado biome is responsible for part of Brazilian food production. However, due to inadequate soil management practices, large areas of crop and forage are degraded, leading to environmental and economic losses. A crop-livestock-forest system (CLFS) is a sustainable production strategy that integrates different cultivations. This objective of this study was to evaluate agronomic characteristics and maize (Zea mayz L.) yield when consorciated with Panicum maximum cv. Tamani, cultivated between rows of eucalypts clones. The statistical design used was randomized blocks, constituted by the following treatments: Maize + AEC 043; Maize + AEC 007; Maize + AEC 2111; and Maize + AEC 2034. The analyzed variables included: spikes length, number of rows, number of grains per row, number of grains per spike, and grain yield. Based on the obtained data, it is possible to conclude that trees did not affect maize yield in a CLFS. Considering that only 66% of the area was used for agriculture, maize yield was higher than when it is planted in total area.

https://doi.org/10.1590/0034-737x202067030002 403 downloads

Genomic detection of a virus lineage replacement event of dengue virus serotype 2 in Brazil, 2019
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de Jesus, Jaqueline Goes

; Dutra, Karina Rocha

; Sales, Flavia Cristina da Silva

; Claro, Ingra Morales

; Terzian, Ana Carolina

; Candido, Darlan da Silva

; Hill, Sarah C

; Thézé, Julien

; Torres, Celeste

; D’Agostini, Tatiana Lang

; Felix, Alvina Clara

; Reis, Andreia F Negri

; Alcantara, Luiz Carlos Junior

; de Abreu, André L

; Croda, Júlio HR

; de Oliveira, Wanderson K

; de Filipis, Ana Maria Bispo

; Camis, Maria do Carmo Rodrigues dos Santos

; Romano, Camila Malta

; Loman, Nick J

; Pybus, Oliver G

; Sabino, Ester Cerdeira

; Nogueira, Mauricio L

; Faria, Nuno Rodrigues

Memórias do Instituto Oswaldo Cruz

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2020, Volume 115 elocation e190423

Abstract: En | Text: En | PDF: En

BACKGROUND Despite efforts to mitigate the impact of dengue virus (DENV) epidemics, the virus remains a public health problem in tropical and subtropical regions around the world. Most DENV cases in the Americas between January and July 2019 were reported in Brazil. São Paulo State in the southeast of Brazil has reported nearly half of all DENV infections in the country. OBJECTIVES To understand the origin and dynamics of the 2019 DENV outbreak. METHODS Here using portable nanopore sequencing we generated20 new DENV genome sequences from viremic patients with suspected dengue infection residing in two of the most-affected municipalities of São Paulo State, Araraquara and São José do Rio Preto. We conducted a comprehensive phylogenetic analysis with 1,630 global DENV strains to better understand the evolutionary history of the DENV lineages that currently circulate in the region. FINDINGS The new outbreak strains were classified as DENV2 genotype III (American/Asian genotype). Our analysis shows that the 2019 outbreak is the result of a novel DENV lineage that was recently introduced to Brazil from the Caribbean region. Dating phylogeographic analysis suggests that DENV2-III BR-4 was introduced to Brazil in or around early 2014, possibly from the Caribbean region. MAIN CONCLUSIONS Our study describes the early detection of a newly introduced and rapidly-expanding DENV2 virus lineage in Brazil.

https://doi.org/10.1590/0074-02760190423 1193 downloads

SARS-CoV-2 diagnostic diary: from rumors to the first case. Early reports of molecular tests from the military research and diagnostic institute of Rio de Janeiro
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Cipitelli, Marcio da Costa

; Valentin, Elizabeth

; da Cruz, Nadia Vaez Gonçalves

; Nogueira, Tatiana LS

; de Melo, Elaine Cristina Amaro

; da Silva, Rebeca Araujo

; Serra, Marcelo M

; Meriano, André L

; Colares, Alberto ML

; Dornelas-Ribeiro, Marcos

; Santos, Caleb GM

Memórias do Instituto Oswaldo Cruz

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2020, Volume 115 elocation e200200

Abstract: En | Text: En | PDF: En

Corona virus disease (COVID-19) presents a serious threat to global health. A historical timeline of early molecular diagnostics from government alert (January 22) (D) was presented. After in silico analysis, Brazilian Army Institute of Biology (IBEx-RJ) tested samples in house using real-time reverse transcriptase polymerase chain reaction (RT-PCR) (fast mode) based on Centers for Disease Control and Prevention (CDC) recommendations. First cases from Brazil, Rio de Janeiro, IBEx, and diagnosis team were reported in D36, D44, D66, and D74 respectively. Therefore, after 1300 tests, we recommend N1/N2 primer sets (CDC) for preliminary and Charité protocol confirmation in case of positive results. Moreover, every professional should be tested before starting work, in addition to weekly tests for everyone involved.

https://doi.org/10.1590/0074-02760200200 1015 downloads

Distillers dried grains with solubles from corn in diet of japanese quails
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Bittencourt, Tatiana Marques

; Lima, Heder José D’Avila

; Valentim, Jean Kaique

; Martins, Ana Carolina da Silva

; Moraleco, Debora Duarte

; Vaccaro, Brenda Chagas

Acta Scientiarum. Animal Sciences

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2019, Volume 41 elocation e42759

Abstract: En | Text: En | PDF: En

ABSTRACT. The objective of this study was to evaluate different inclusion levels of distillers dried grains with solubles (DDGS) from corn in the diet of quails. A total of 210 japanese quails (Coturnix coturnix japonica) were distributed in a completely randomized design of five treatments and six replicates, with seven quails per experimental unit. The treatments used were: control feed; inclusion of 5, 10, 15 and 20% of DDGS. The variables assessed were: feed intake, feed conversion per dozen and egg mass, egg production, egg weight, specific gravity, yolk color and economic analysis. Data were analyzed by regression model and Dunnett’s test at 5% probability. For individual feed intake, laying rate, and feed conversion per egg mass, specific gravity and yolk color by color fan, there was effect caused by inclusion of DDGS (p < 0.05), but the other variables did not show significant difference. By Dunnett’s test, for feed intake, feed conversion per egg mass, specific gravity and digital colorimeter’s L parameter, there was effect (p < 0.05). As for economic analysis, DDGS presented low cost. It can be concluded that DDGS can be used in feed at a 20% level without impairing the performance and quality of quails eggs.

https://doi.org/10.4025/actascianimsci.v41i1.42749 666 downloads

Growth and gas exchange in cowpea plants under different managements and saline conditions
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Silva, Weslley Costa

; Moura, Joana Gomes de

; Oliveira, Alexandre Bosco de

; Ferreira, Leonardo Elias

; Silva, Tatiana Maria da

Revista Ciência Agronômica

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2017, Volume 48 N. 5spe Pages 756 - 764

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O feijão-caupi é uma das principais culturas de importância econômica e social para o Nordeste do Brasil. Entretanto, a crescente necessidade de aumentar a produção da cultura tem motivado a utilização de águas com elevados teores de sais na irrigação. Desta forma o objetivo deste trabalho foi avaliar os efeitos de diferentes lâminas de irrigação e doses de biofertilizante líquido sobre o crescimento e as trocas gasosas de plantas de feijão-caupi em condições salinas. O experimento foi conduzido no delineamento de blocos casualizados, utilizando o esquema de parcelas subdivididas, sendo as parcelas constituídas por quatro lâminas de irrigação com água salina (75; 100; 125 e 150% da evapotranspiração da cultura - ETc) e nas subparcelas quatro diferentes doses de biofertilizante (0; 5.000; 10.000 e 15.000 L ha-1). Aos 75 dias após a semeadura (DAS) foram realizadas leituras das trocas gasosas das plantas (fotossíntese, condutância estomática, concentração interna de CO2 e transpiração) e análises de crescimento (altura de planta, área foliar e matéria seca total). O aumento da lâmina de irrigação proporciona incremento na massa seca total e área foliar. Para as trocas gasosas, o aumento da lâmina de irrigação proporciona aumento significativo, sendo verificado comportamento quadrático para a fotossíntese e condutância estomática, e linear para concentração interna de CO2 e transpiração. A aplicação de elevadas doses de biofertilizante proporciona aumento na abertura estomática das folhas de feijão-caupi.

ABSTRACT The cowpea is one of the main crops of economic and social importance in the northeast of Brazil. However, the growing need to increase crop yield has prompted the use of irrigation water with high levels of salts. The aim of this study therefore, was to evaluate the effects of different irrigation depths and rates of liquid biofertilizer on growth and gas exchange in cowpea plants under saline conditions. The experiment was conducted in a randomized block design, using a scheme of split lots consisting of four irrigation depths with saline water (75, 100, 125 and 150% of crop evapotranspiration - ETc) and sub-lots of four different doses of biofertilizer (0; 5,000; 10,000 and 15,000 L ha-1). At 75 days after sowing (DAS), readings were taken of gas exchange in the plants (photosynthesis, stomatal conductance, internal CO2 concentration and transpiration), and growth analyses were carried out (plant height, leaf area and total dry matter). An increase in irrigation depth gives an increase in total dry weight and leaf area. Increasing irrigation depth causes a significant increase in gas exchange, with quadratic behavior noted for photosynthesis and stomatal conductance, and linear behavior for internal CO2 concentration and transpiration. The application of high doses of biofertilizer gives increased stomatal opening of the cowpea leaves.

https://doi.org/10.5935/1806-6690.20170088 873 downloads

Morphometric and Fractal Analysis of Injured Skeletal Muscle Tissue Subjected to A Combination of Treatments; Cryotherapy and Therapeutic Ultrasound
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Koike, Tatiana Emy

; Camargo, Regina Celi Trindade

; Ozaki, Guilherme Akio Tamura

; Castoldi, Robson Chacon

; Seraphim, Patrícia Monteiro

; Oikawa, Sérgio Minoro

; Camargo Filho, José Carlos Silva

International Journal of Morphology

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sep 2016, Volume 34 N. 3 Pages 1076 - 1082

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

The aim of this study was to analyze the effects of an association of cryotherapy and therapeutic ultrasound on the treatment of muscle injured by impact. Fifty-five Wistar rats were divided into five groups (n = 11), Acute Injury (AI), Injury (I), Cryotherapy (CR), Therapeutic Ultrasound (TU) and Association of Cryotherapy and Therapeutic Ultrasound (CRTU). The CR and CRTU groups received applications of Cryotherapy three times (immediately, 24 and 48 h after injury) of 20 minutes duration. The TU and CRTU groups received applications of Therapeutic Ultrasound for seven days, for five minutes, in pulsed mode, 0.5 w/cm intensity, frequency 1 MHz. Body mass and gastrocnemius mass were analyzed. In addition to histological slides stained with hematoxylin and eosin used for morphometric analysis, picrosirius dye was used for quantification of collagen by Fractal Dimension (FD). The results of the intra-group analysis showed lower body mass and gastrocnemius in the CRTU group in relation to the AI (p = 0.001), I (p = 0.001), CR (p = 0.001) and TU groups (p = 0.001), and lower values of FD to quantify collagen in the CRTU group in relation to the AI (p = 0.007) and CR groups (p = 0.014). In summary, the present study showed that the association of Cryotherapy with Therapeutic Ultrasound promoted better results in the aspects analyzed compared to application of the therapies in isolation.

El objetivo del estudio fue evaluar los efectos de la asociación de las técnicas de crioterapia y ultrasonido terapéutico en el tratamiento de la lesión muscular por impacto. Fueron utilizadas 55 ratas Wistar, expuestas a lesión y separadas en grupos (n = 11): Lesión aguda (LA), Lesión (L), Crioterapia (CR), Ultrasonido Terapéutico (UT) y Crioterapia + Ultrasonido Terapéutico (CRUT). Los grupos CR y CRUT recibieron la aplicación, durante 20 minutos, en tres momentos (inmediatamente, 24 y 48 horas, después de la lesión). Los grupos UT y CRUT, recibieron UT por siete días, con una duración de cinco minutos, en modo pulsado, con una intensidad de 0,5 W/cm2 y frecuencia de 1 MHz. Fueran medidos el peso corporal y el peso de los músculos gastrocnemios y se realizaron cortes histológicos del músculo gastrocnemio, los cuales fueron teñidos con hematoxilina-eosina (HE) para el análisis morfométrico y con picrosirius para el análisis del colágeno por dimensión fractal (DF). Los resultados de los análisis intragrupo demostraron una menor disminución de la masa coporal y muscular en el grupo CRUT. Además, fue observado un valor inferior en la morfometría en el grupo CRUT en comparación a los grupos LA (p = 0,001), L (p = 0,001), CR (p = 0,001) y UT (p = 0,001), y un menor valor de la DF con respecto al colágeno en el grupo CRUT en comparación a los grupos LA (p = 0,007) y CR (p = 0,014). En síntesis, el presente estudio demostró que el protocolo de asociación de las técnicas de CR y UT causaron mayores respuestas benéficas en los aspectos analizados en comparación a los protocolos con los tratamientos aplicados de forma aislada.

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Allelopathy of Crotalariajuncea L. aqueous extracts on germination and initial development of maize
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Araujo da Cruz-Silva, Claudia Tatiana

; Becker Matiazzo, Ediane

; Palczewski Pacheco, Fábio

; Pereira Nóbrega, Lúcia Helena

Idesia (Arica)

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feb 2015, Volume 33 N. 1 Pages 27 - 32

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

The objective of this study was to evaluate the allelopathic potential of sunn hemp extracts on germination and initial development of maize. The trials were carried out in the laboratory and greenhouse with four concentrations (0; 7.5; 15 and 30%) of aqueous extracts obtained by grinding and leaching. In a germination chamber, more concentrated extracts (15% and 30%) inhibited maize germination, while shoot length (SL) was stimulated at 7.5% concentration. Ground extract stimulated root length (RL) at all tested concentrations. Root number (RN) was inhibited at higher concentrations of leached extract and stimulated for seeds exposed to sunn hemp ground extract. In the greenhouse, the longest root length (LRL) of maize was inhibited at the highest concentration (30%) of both extracts. The ground extract inhibited the LS in all concentrations and the 7.5% concentration stimulated the RN. It was concluded that sunn hemp extracts showed allelopathic effect on maize development, which varied according to how the extracts were obtained and concentrations used.

El objetivo de la investigación fue evaluar el potencial alelopático de los extractos de crotalaria sobre la germinación y el crecimiento inicial de maíz. Los ensayos se realizaron en el laboratorio y en el invernadero con cuatro concentraciones (0; 7,5; 15 y 30%) para los extractos acuosos obtenidos por molienda y lixiviación. En cámara de la germinación se observó que los extractos más concentrados (15 y 30%) inhibió la germinación de maíz y que la longitud de la parte aérea (LPA) se estimuló a una concentración de 7,5%. Para el extracto de molienda hubo estimulación en longitud de la raíz (LR) en todas las concentraciones ensayadas. El número de raíces (NR) se inhibió a concentraciones más altas de extracto de lixiviado y estimuló para las semillas expuestas a extracto machacado de crotalaria. En invernadero, la longitud de la raíz más larga (LRL) de maíz se inhibió a la mayor concentración (30%) de los extractos de ambas maneras obtenidos. El extracto machacado inhibió los LPA en todas las concentraciones y la concentración de 7,5% estimuló la NR. Se concluyó que los extractos de crotalaria mostraron efecto alelopático en el desarrollo de maíz, que variaba de acuerdo a cómo se obtuvieron los extractos y concentraciones utilizadas.

1985 downloads

Fibrinolytic protease production by new Streptomyces sp: DPUA 1576 from Amazon lichens
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M.M. Silva, Germana

; Bezerra, Raquel P

; Teixeira, José A

; Porto, Tatiana S

; Lima-Filho, José L

; Porto, Ana Lúcia F

Electronic Journal of Biotechnology

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jan 2015, Volume 18 N. 1 Pages 16 - 19

Abstract: En | Text: En | PDF: En

Background Streptomyces sp. DPUA 1576 from Amazon lichens was studied to protease and fibrinolytic production. A 2² factorial experimental design was applied to optimize its protease enzyme production using two independent variables, namely soybean flour and glucose concentrations. Results The optimal conditions to obtain high protease production (83.42 U/mL) were 1.26% soybean flour and 1.23% glucose concentration. A polynomial model was fitted to correlate the relationship between the two variables and protease activity. In relation to fibrinolytic activity, the highest activity of 706.5 mm² was obtained at 1.7% soybean flour and 1.0% glucose concentration, which was 33% higher than plasmin. Fibrinolytic production was not optimized in the studied conditions. Conclusions These results show that the optimization of the culture medium can enhance protease production, thus becoming a good process for further research. In addition, Streptomyces sp. DPUA 1576, isolated from Amazon lichens, might be a potential strain for fibrinolytic protease production.

https://doi.org/10.1016/j.ejbt.2014.11.001 1402 downloads

Cultivo do mirtilo: atualizações e desempenho inicial de variedades de baixa exigência em frio no Estado de Säo Paulo
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Cantuarias-Avilés, Tatiana

; Silva, Simone Rodrigues da

; Medina, Ricardo Bordignon

; Moraes, Ana Flavia Garcia

; Alberti, Mariana Freire

Revista Brasileira de Fruticultura

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mar 2014, Volume 36 N. 1 Pages 139 - 147

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A produção comercial de mirtilos na América do Sul tem passado por mudanças importantes nos últimos anos, em função do aumento da oferta de frutos frescos dos principais países produtores, causando forte queda de preços em plena safra, entre os meses de dezembro e fevereiro. Uma alternativa para esta situação é a produção de mirtileiros de maturação precoce ou muito tardia, fora da época de meia-estação, caracterizada pelos menores preços da fruta. Em 2010, foram introduzidas no Brasil novas variedades de mirtileiros de baixa exigência em frio desenvolvidas pela Universidade da Flórida, que podem ser cultivadas em regiões quentes e apresentam uma frutificação mais precoce, quando comparadas com as variedades tradicionalmente cultivadas no Brasil. Essas novas variedades apresentam grande potencial para a produção precoce de mirtilos no País em regiões sem frio hibernal, aumentando significativamente a rentabilidade da cultura. Para viabilizar esta produção, é necessário validar estratégias de manejo para estas variedades, devido à falta de informação técnica sobre seu cultivo nas condições edafoclimáticas brasileiras. Atualmente, estäo sendo conduzidas as primeiras pesquisas fitotécnicas no Estado de Säo Paulo para avaliar o crescimento e a produção destas variedades na ausência total de frio, situação de ocorrência particular em poucos países produtores do mundo.

In recent years, South American commercial blueberry production has gone through important changes imposed by a larger offer of fresh fruit from the main producers, thus leading to drastic price reductions during main harvest season, from December to February. One alternative solution to this situation is to grow early or late fruit ripening varieties, harvested at the beginning or at the end of the harvest period. In 2010 new blueberry varieties with low winter chilling requirements were introduced to Brazil, which were developed by the University of Florida and may be planted under hot weather conditions, showing earlier fruit bearing when compared to the traditional varieties grown in Brazil so far. Those low chilling requirement blueberries have an undeniable potential for early fruit production in hot regions with no chill hours during winter, with increased crop profitability to growers. For an adequate establishment of these varieties in Brazil, crop management strategies should be validated in regions with no chill hours, due to the lack of technical information on blueberry crop under such particular conditions. First field trials with early-bearing blueberry varieties are currently being conducted in the State of Säo Paulo for evaluating plant growth and fruit yield and quality under no chilling incidence, being these exceptional conditions met by few countries in the world.

https://doi.org/10.1590/0100-2945-453/13 6863 downloads Cited 5 times in SciELO

10.

Avaliação da promoção do aleitamento materno em Hospitais Amigos da Criança
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Souza, Mateus Freire L

; Ortiz, Priscilla Nunes

; Soares, Poliana Louzada

; Vieira, Tatiana de Oliveira

; Vieira, Graciete Oliveira

; Silva, Luciana Rodrigues

Revista Paulista de Pediatria

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dec 2011, Volume 29 N. 4 Pages 502 - 508

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

OBJETIVO: Avaliar o cumprimento dos Passos 4 a 10 dentre os Dez Passos para o Sucesso do Aleitamento Materno, preconizados pela Iniciativa Hospital Amigo da Criança (IHAC), criada pela Organização Mundial da Saúde (OMS). MÉTODOS: Estudo descritivo de corte transversal. Foram realizadas entrevistas com 100 puérperas nos dois hospitais credenciados pela IHAC em Salvador. Não foram incluídas mães ou recém-nascidos que não poderiam oferecer ou receber aleitamento materno exclusivo, respectivamente.Questionou-se sobre história obstétrica, aleitamento materno anterior, atendimento pré-natal e aspectos relacionados aos Passos Para o Sucesso do Aleitamento Materno. As questões foram elaboradas de acordo com os Critérios Globais para a IHAC. No mínimo 80% das mães deveriam responder de forma satisfatória às perguntas correspondentes a cada passo para que ele fosse considerado como cumprido. Foi feita descrição de frequências para avaliar as respostas. RESULTADOS: O cumprimento foi insatisfatório para o Passo 4 (suporte ao aleitamento materno após o parto - 58%), Passo 5 (aleitamento exclusivo durante a internação - 77%) e Passo 10 (encaminhamento para grupo de suporte ao aleitamento materno - 5%). Outros passos demonstraram bons resultados: Passo 6 (oferta de substitutos do leite materno - 19%), Passo 7 (prática do alojamento conjunto - 91%) e Passo 9 (não uso de chupetas e mamadeiras - 100%). CONCLUSÕES: Houve boa aderência a alguns aspectos dos Critérios Globais da IHAC. Evidencia-se, no entanto, a necessidade de se ampliarem as discussões sobre os critérios para manter o título de "Hospital Amigo da Criança", uma vez que os resultados foram insatisfatórios em relação aos Passos 4, 5 e 10.

OBJECTIVE: To evaluate the compliance to steps 4 to 10 of the Ten Steps Program to Successful Breastfeeding recommended by the Baby-Friendly Hospital Initiative (BFHI), created by World Health Organization (WHO). METHODS: Cross-sectional descriptive study of 100 mothers in the immediate post-partum period admitted to both BFHI accredited hospitals in Salvador (Northeast Brazil). Newborns that could not be exclusively breastfed were not included. The mothers were questioned about obstetric history, previous breastfeeding, prenatal care, and aspects related to The Ten Steps to Successful Breastfeeding. Interviewers used a questionnaire based on the BFHI Global Criteria. At least 80% of the mothers had to satisfactorily answer the questions related to each step in order to consider them complied. Descriptive statistics was used to evaluate the answers. RESULTS: The compliance was unsatisfactory for Step 4 (support to breastfeeding initiation immediately after birth - 58%), Step 5 (exclusive breastfeeding during hospitalization - 77%), and Step 10 (referral of mothers to a breastfeeding support group following discharge from hospital - 5%). Other steps showed a satisfactory result: Step 6 (offer of formula - 19%), Step 7 (practice of rooming-in - 91%) and Step 9 (no pacifiers and bottles use - 100%). CONCLUSIONS: A satisfactory compliance with some aspects of the BFHI Global Criteria was noted, but more discussions about strategies to keep the title of "Baby Friendly Hospital" are needed, since the compliance was poor regarding Steps 4, 5 and 10.

OBJETIVO: Evaluar el cumplimiento de los Pasos 4 a 10 entre los Diez Pasos para el Éxito de la Lactancia Materna, preconizados por la Iniciativa Hospital Amigo del Niño (IHAC), creada por la OMS. MÉTODOS: Estudio descriptivo de corte transversal. Fueron realizadas entrevistas con 100 puerperas en los dos hospitales acreditados en la IHAC en Salvador (Brasil). Se cuestionó sobre la historia obstétrica, lactancia materna anterior, atención prenatal y aspectos relacionados a los Pasos para el Éxito de la Lactancia Materna. Las cuestiones fueron elaboradas conforme a los Criterios Globales para la IHAC. Como mínimo, el 80% de las madres deberían contestar de modo satisfactorio a las preguntas correspondientes a cada paso para que se lo considerara cumplido. Se utilizó la descripción estadística de frecuencia en el SPSS 13.0 para evaluar las respuestas. No se incluyeron madres o recién nacidos que no podrían recibir lactancia materna exclusiva. RESULTADOS: El cumplimiento fue insatisfactorio para: Paso 4 (soporte a la lactancia materna después del parto - 58%), Paso 5 (lactancia materna exclusiva durante la internación - 77%) y Paso 10 (encaminamiento al grupo de apoyo a la lactancia materna - 5%). Otros pasos demostraron buenos resultados: Paso 6 (oferta de sustitutos de la leche materna - 19%), Paso 7 (práctica del alojamiento conjunto - 91%) y 9 (no utilización de chupos y biberones - 100%). CONCLUSIONES: Esos resultados demostraron buena adherencia a algunos aspectos de los Criterios Globales de IHAC. Se evidencia, sin embargo, la necesidad de ampliar las discusiones sobre los criterios para obtener y mantener el prestigioso título de «Hospital Amigo del Niño», una vez que los resultados fueron insatisfactorios respecto a los pasos 4, 5 y 10.

11591 downloads

11.

New insights into trypanosomatid U5 small nuclear ribonucleoproteins
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Silva, Marco Túlio A da

; Ambrósio, Daniela L

; Trevelin, Caroline C

; Watanabe, Tatiana F

; Laure, Helen J

; Greene, Lewis J

; Rosa, José C

; Valentini, Sandro R

; Cicarelli, Regina MB

Memórias do Instituto Oswaldo Cruz

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mar 2011, Volume 106 N. 2 Pages 130 - 138

Abstract: En | Text: En | PDF: En

Several protozoan parasites exist in the Trypanosomatidae family, including various agents of human diseases. Multiple lines of evidence suggest that important differences are present between the translational and mRNA processing (trans splicing) systems of trypanosomatids and other eukaryotes. In this context, certain small complexes of RNA and protein, which are named small nuclear ribonucleoproteins (U snRNPs), have an essential role in pre-mRNA processing, mainly during splicing. Even though they are well defined in mammals, snRNPs are still not well characterized in trypanosomatids. This study shows that a U5-15K protein is highly conserved among various trypanosomatid species. Tandem affinity pull-down assays revealed that this protein interacts with a novel U5-102K protein, which suggests the presence of a sub-complex that is potentially involved in the assembly of U4/U6-U5 tri-snRNPs. Functional analyses showed that U5-15K is essential for cell viability and is somehow involved with the trans and cis splicing machinery. Similar tandem affinity experiments with a trypanonosomatid U5-Cwc21 protein led to the purification of four U5 snRNP specific proteins and a Sm core, suggesting U5-Cwc-21 participation in the 35S U5 snRNP particle. Of these proteins, U5-200K was molecularly characterized. U5-200K has conserved domains, such as the DEAD/DEAH box helicase and Sec63 domains and displays a strong interaction with U5 snRNA.

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12.

BAP, 2,4-D e ácido acetilsalicílico na indução e diferenciação de calos em anteras de Coffea arabica L
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Silva, Adelaide Siqueira

; Luz, José Magno Queiroz

; Rodrigues, Tatiana Michlovská

; Bittar, Cecília Alves

; Lino, Leandro de Oliveira

Ciência e Agrotecnologia

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oct 2009, Volume 33 N. 5 Pages 1205 - 1212

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O melhoramento genético do cafeeiro por meio de métodos convencionais é um processo demorado para se obter uma nova cultivar. A redução desse tempo é possível através da produção de linhagens homozigóticas, oriundas de dihaplóides obtidas através da cultura de anteras. Objetivou-se aplicar a técnica da cultura de anteras em diferentes cvs. de Coffea arabica L. para induzir a formação de calos e regenerar plântulas di-haplóides, com uso de reguladores vegetais. Os experimentos foram conduzidos no laboratório de Biotecnologia Vegetal da Universidade Federal de Uberlândia (UFU). Anteras das cultivares Mundo Novo LCP-379-19 e Catuaí Vermelho H2077-2-5-44 foram inoculadas em meio MS suplementado com 2,0 mg L-1 de 2,4-D e AAS, nas concentrações de 0; 8; 16; 32 e 64 mg L-1. Calos de 'Catuaí Vermelho 44' foram subcultivados em meio MS acrescido de diferentes concentrações de BAP (0; 2; 4 e 8 mg L-1) e 2,4-D (0; 1; 2 e 4 mg L-1). Tanto para as cvs. Mundo Novo quanto para Catuaí Vermelho 44 o aumento das concentrações de AAS diminuiu a formação de próembrióides nos calos e somente o 2,4-D foi capaz de promover a formação de calos friáveis, porém o equilíbrio da auxina e da citocinina utilizadas no trabalho, favoreceram a produção de calos friáveis.

Coffee plant breeding through conventional methods demands a long time to obtain new cultivars. The reduction of this period is possible through the production of homozygous lines, from dihaploids obtained via anther culture. The aim of this study was to apply the anther culture technique on different C. arabica L. cultivars to induce calli formation and to regenerate dihaploid seedlings with the use of plant growth regulators. The experiments were accomplished in the Plant Biotechnology laboratory at Uberlândia Federal University (UFU). Anthers of the cultivars Mundo Novo LCP-379-19 and Catuaí Vermelho H2077-2-5-44 were inoculated on MS medium supplemented with 2.0 mg L-1 2,4-D and ASA at 0, 8, 16, 32 or 64 mg L-1. 'Catuaí Vermelho 44' calli were subcultured on MS supplemented with different concentrations of BAP (0, 2, 4 or 8 mg L-1) and 2,4-D (0, 1, 2 or 4 mg L-1). The increase in ASA concentrations decreased the pro-embryoid formation on calli of both cultivars and only 2,4-D promoted the formation of friable calli. However, the balance of auxin and cytokinin used in this study favored the production of friable calli.

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13.

Calibração de câmara semiaberta estática para quantificação de amônia volatilizada do solo
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Araújo, Ednaldo da Silva

; Marsola, Tatiana

; Miyazawa, Mário

; Soares, Luís Henrique de Barros

; Urquiaga, Segundo

; Boddey, Robert Michael

; Alves, Bruno José Rodrigues

Pesquisa Agropecuária Brasileira

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jul 2009, Volume 44 N. 7 Pages 769 - 776

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O objetivo deste trabalho foi calibrar uma câmara prática e de baixo custo, para quantificar a amônia volatilizada do solo. A calibração da câmara foi realizada pela técnica do balanço do isótopo 15N. A amônia volatilizada foi capturada por uma câmara semiaberta livre estática (SALE), confeccionada a partir de garrafa de plástico transparente de politereftalato de etileno (PET) de 2 L. O interior da câmara apresentava livre circulação de ar, e possuía uma lâmina de espuma de poliuretano, com 2,5 cm de largura e 25 cm de comprimento, pendurada verticalmente, com a extremidade inferior inserida em frasco com solução ácida, que mantinha a espuma saturada. A recuperação do 15N-NH3 no coletor aumentou de forma linear com o aumento da taxa de volatilização de NH3 do solo. Os testes de campo mostraram eficiência de 57% na recuperação do N-NH3 volatilizado do solo. O intervalo de coletas das espumas pode variar de um a seis dias, sem comprometer a eficiência do sistema. Para estudos de campo, com uso do método proposto, o fator de correção de 1,74 deve ser usado para estimar a real taxa de volatilização de amônia do solo.

The objective of this work was to calibrate a practical and low-cost chamber for the quantification of ammonia volatilization from soil. The chamber was calibrated using the 15N isotope balance technique. The volatilized ammonia was captured by a semi-opened free static chamber built with a 2 L ethylene polyterephthalate (PET) plastic bottle. The inner side of the chamber had free-air circulation and a stripe of polyurethane foam, with 2.5 cm wide and 25 cm long, hanged vertically with the lower end inserted in a bottle containing acid solution, which kept the foam moistened. The recovery of 15N-ammonia by the chamber increased linearly with the increase in the rate of soil ammonia volatilization. Field tests showed an efficiency of 57% in the volatilized ammonia recuperation. The sampling interval of foam can vary from one to six days without impairing the system efficiency. For studies in the field using the proposed method, a correction factor of 1.74 should be used to estimate the real rate of ammonia volatilization from soil.

5619 downloads Cited 9 times in SciELO

14.

Bioactivity of crude ethanol extract and fractions of Eugenia uniflora (Myrtaceae) in the hepatopancreas of Oreochromis niloticus L
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FIUZA, TATIANA S

; SILVA, PAULO C

; PAULA, JOSÉ R DE

; TRESVENZOL, LEONICE M F

; SABÓIA-MORAIS, SIMONE M T

Biological Research

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2009, Volume 42 N. 4 Pages 401 - 414

Abstract: En | Text: En | PDF: En

This study evaluates the bioactivity of the crude ethanol extract and ethyl acetate, hexane and chloroform fractions obtained from Eugenia uniflora leaves using the hepatopancreas of Oreochromis niloticus L. as an experimental model. The ethanol extract and fractions were administered to the fish orally with their feed. Twenty-four hours later, the fish were sacrificed and their livers dissected, fixed in neutral formalin, embedded in paraffin and sectioned. Histological analyses were performed using Masson's trichrome and Haematoxylin-Eosin. Histochemical studies were performed using Feulgen, PAS (Periodic Acid Schiff) and PAS + salivary amylase and Sudan IV stain. The qualitative analysis of the material showed that the crude extract and the ethyl, chloroform and hexane fractions induced vasodilation, vascular congestion and toxicity due to the presence of eosinophilic granular cells, rodlet cells, some leukocytic infiltrate and rare focal necroses. The Nile tilapia proved to be a satisfactory model for screening plant products.

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15.

The effect of crude ethanol extract and fractions of Hyptidendron canum (Pohl ex Benth.) Harley on the hepatopancreas of Oreochromis niloticus L
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FIUZA, TATIANA S

; SILVA, PAULO C

; PAULA, JOSÉ R

; TRESVENZOL, LEONICE M F

; SABÓIA-MORAIS, SIMONE M T

Biological Research

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2009, Volume 42 N. 2 Pages 153 - 162

Abstract: En | Text: En | PDF: En

Hyptidendron canum (Pohl ex Benth.) Harley is a native tree of the Brazilian Savannah. The fish Oreochromis niloticus L. was used as an experimental model to determine the bioactivity of the crude ethanol extract as well as ethyl acetate, hexanic and chloroform fractions obtained from its leaves. The plant ethanol extract and fractions were administered to the fish orally with their feed. Twenty four hours later, the fish were sacrificed and their livers dissected, fixed in neutral formalin, embedded in paraffin and sectioned. Histological analyses were performed using Masson's trichrome and Haematoxylin-Eosin. Histochemical studies were performed using Feulgen, PAS (Periodic Acid Schiff) and PAS + salivary amylase and Sudan IV stain. The qualitative analysis of the material showed that both the crude ethanol extract and the fractions from H. canum induced vasoactive activity, causing vasodilation and vascular congestion, and the hexanic fraction also caused an apparent proliferation of capillaries. Hepatopancreas toxicity was evident through inflammatory processes. Pancreatic (chloroform fraction) and hepatic alterations, hemorrhagic spots and necroses were observed in fish treated with-ethanol extract and fractions. This study is the first description of the biologic action of the crude ethanol extract and the hexane, ethyl acetate and chloroform fractions in fish.

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