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au:SOUZA, G.M.

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Carbothermic reduction of a willemite concentrate for use in the Waelz process
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Coimbra, V.S.

; de Lima, G.M.

; Leão, V.A.

; de Souza, R.F.M.

; Oliveira, V.A.A.

Journal of the Southern African Institute of Mining and Metallurgy

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nov 2023, Volume 123 N. 11 Pages 513 - 520

Abstract: En | Text: En | PDF: En

A willemite concentrate consisting mainly of willemite (55.3%) and dolomite (15.6%) was reduced in a tube furnace with charcoal as reductant. The effects of temperature and varying reductant additions on zinc extraction were studied. A zinc recovery of 86% was achieved with 20% reductant in the charge at 1100°C. During reduction, the calcium oxide generated through calcination of the dolomite promoted decomposition of the willemite, thus lowering the temperature at which reduction took place. The presence of CaO improved the zinc recovery at all temperatures studied. The best zinc recovery (93%) was obtained in the presence of 5% CaO, 20% charcoal reductant, and at a temperature of 1100°C. The results show that the willemite concentrate studied has great potential to be used as a raw material for the Waelz process.

https://doi.org/10.17159/2411-9717/2516/2023

Intraoperative clonidine to prevent postoperative emergence delirium following sevoflurane anesthesia in pediatric patients: a randomized clinical trial
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Sousa-Júnior, Fernando A.

; Souza, Alex S.R.

; Lima, Luciana C.

; Santos, Ítalo G.M.

; Menezes, Leonardo A.P.

; Ratis, Pedro A.P.L.

; Couceiro, Tania C.M.

Brazilian Journal of Anesthesiology

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feb 2021, Volume 71 N. 1 Pages 5 - 10

Abstract: En | Text: En | PDF: En

Abstract Introduction and objective Emergence Delirium (ED), particularly in children, is characterized by mental confusion, irritability, disorientation, and inconsolable crying. ED prolongs the time required in the Post-Anesthesia Care Unit (PACU) and increases concern and anxiety in parents. The present study aimed to determine the effectiveness and safety of low-dose clonidine in preventing ED in children receiving sevoflurane anesthesia for tonsillectomy/adenotonsillectomy. Methods A randomized, double-blind clinical trial was conducted between November 2013 and January 2014. Sixty-two children aged 2-12 years, scheduled to undergo tonsillectomy/adenotonsillectomy, and classified as American Society of Anesthesiologists (ASA) physical status I/II were included, with 29 being randomized to receive 1 µg.kg-1 clonidine intravenously, and 33 allocated to a control group that received no clonidine. Anesthesia was induced and maintained with sevoflurane. Children with altered state of consciousness, neurological deficit, history of allergy to dipyrone, or receiving other drugs such as preanesthetic agents were excluded from the study. The primary outcome was the presence of ED in the initial 20 minutes in the PACU according to the Pediatric Anesthesia Emergence Delirium (PAED) scale. The Chi-Square test and Fisher’s two-tailed exact test were used for statistical analysis, as applicable. Significance level was set at 5%, and Risk Ratios (RR) and their 95% Confidence Intervals (95% CI) were calculated. Results The frequency of ED was significantly decreased in the group of children who received clonidine (17.2% vs. 57.6%; RR = 0.30; 95% CI 0.13-0.70; p= 0.001). There was no difference between groups with respect to the frequency of postoperative self-harm (falls and bruises), dislodged catheters, and for most of the other adverse events evaluated. Conclusions The use of 1 µg.kg-1 intravenous clonidine during anesthesia induction can effectively reduce the incidence of ED in children undergoing elective tonsillectomy/adenotonsillectomy under general inhalation anesthesia with sevoflurane. ClinicalTrials.gov Identifier NCT02181543.

https://doi.org/10.1016/j.bjane.2020.12.003 100 downloads

Bases para um programa de controle da artrite encefalite caprina em rebanho leiteiro
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Alves, R.P.A.

; Rodrigues, A.S.

; Santos, V.W.S.

; Damasceno, E.M.

; Prado, G.M.

; Souza, K.C.

; Nunes Neto, T.B.

; Pinheiro, A.A.

; Cruz, M.S.P.

; Pinheiro, R.R.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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dec 2020, Volume 72 N. 6 Pages 2053 - 2058

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivou-se avaliar um programa de controle da artrite encefalite caprina (AEC), por meio de testes diagnósticos sensíveis, separação de mãe e cria após o parto e medidas de manejo, com o intuito de formar rebanho livre do vírus. Utilizou-se um total de 47 cabritos da raça Saanen, mantidos isoladamente até o resultado dos primeiros testes de reação em cadeia de polimerase nested (PCR nested) e Western Blotting (WB), com base na coleta de sangue no momento do nascimento (M0). No PCR nested, quatro animais foram positivos, no M0, e foram eutanasiados. Posteriormente, os demais 43 cabritos foram submetidos à coleta de sangue aos 60 (M60) e 270 (M270) dias de vida para realização de novos testes de WB e PCR nested, que não detectaram animais positivos. Pode-se afirmar que a metodologia adotada neste estudo foi efetiva no controle da doença, nas fases de aleitamento e pós-aleitamento, e que a combinação do sistema de manejo, a fim de propiciar diminuição de risco de transmissão horizontal, com técnicas de diagnóstico mais apuradas, como o WB e a PCR nested, é relevante para elaboração de plano estratégico de controle da enfermidade.

ABSTRACT We aimed to evaluate a program to control Caprine Arthritis Encephalitis (CAE), using diagnostic tests, separation of the mother and postpartum and other management measures, in order to form a free flock of the virus. We used a total of 47 Saanengoats in isolation until the results of the first nested Polymerase Chain Reaction (nested PCR) and Western Blotting (WB) tests, based on blood collection at the time of birth (M0). In the nested PCR, 4 animals were positive, at M0, and were eliminated. Later, the other 43goats were submitted to blood collection at 60 (M60) and 270 (M270) days of life to perform new tests of WB and nested PCR, which did not detect positive animals. We can affirm that the methodology adopted in this study was effective in the control of the disease, in the phase of breastfeeding and post-breastfeeding, and that the combination of the management system, which allows a reduction of risk of horizontal transmission, with more accurate diagnostic techniques, such as WB and nested PCR, is relevant for the elaboration of a strategic plan for the disease control.

https://doi.org/10.1590/1678-4162-11695 639 downloads

200.000 IU of vitamin D does not reduce resting Blood Pressure and Inhibit Post-Exercise Hypotension in elderly women: a pilot study
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GONÇALVES, MARIA DA CONCEIÇÃO R.

; MIRANDA NETO, MANOEL

; CAVALCANTE, ISA G.M.

; SEBADELHE, VITTÓRIA R.R.J.

; SOUZA, MICHELE F. De

; NEVES, JULIANA P.R.

; SOUZA, FLAVIA T.L. DE

; TAVARES, RENATA L.

; COSTA, MATHEUS S.

; SILVA, ALEXANDRE S.

Anais da Academia Brasileira de Ciências

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2020, Volume 92 N. 1 elocation e20190227

Abstract: En | Text: En | PDF: En

Abstract Given the scarcity of studies with elderly and the existence of studies investigating the effect of vitamin D supplementation in PEH (post exercise hypotension), this study evaluated the effect of a single megadose of vitamin D on resting blood pressure (RBP) and post-exercise hypotension (PEH) in the elderly. 11 hypertensive elderly women (70.3 ± 1.7 years) received a single megadose of 200.000 IU of cholecalciferol or a placebo, orally, through capsules. On day 7, the subjects performed 30 minutes of aerobic exercise with blood pressure measurement before exercise and every 10 minutes after exercise during 60 minutes, besides cardiac autonomic modulation. RBP did not significantly change. Exercise promoted significant systolic PEH only in one moment post exercise in treated group and in the placebo group promoted significant systolic PEH at four moments. Significant diastolic PEH did not occur in any of the groups. Sympathovagal activity increased at post exercise balance in supplemented subjects at 20 min, 40 min, 50 min and 60 min when compared to rest; this increase was not observed in the placebo. A megadose of vitamin D did not reduce RBP, promoted partial inhibition of systolic PEH and increased sympathovagal balance.

https://doi.org/10.1590/0001-3765202020190227 1055 downloads

Where do we aspire to publish? A position paper on scientific communication in biochemistry and molecular biology
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Baptista, M.S.

; Alves, M.J.M.

; Arantes, G.M.

; Armelin, H.A.

; Augusto, O.

; Baldini, R.L.

; Basseres, D.S.

; Bechara, E.J.H.

; Bruni-Cardoso, A.

; Chaimovich, H.

; Colepicolo Neto, P.

; Colli, W.

; Cuccovia, I.M.

; Da-Silva, A.M.

; Di Mascio, P.

; Farah, S.C.

; Ferreira, C.

; Forti, F.L.

; Giordano, R.J.

; Gomes, S.L.

; Gueiros Filho, F.J.

; Hoch, N.C.

; Hotta, C.T.

; Labriola, L.

; Lameu, C.

; Machini, M.T.

; Malnic, B.

; Marana, S.R.

; Medeiros, M.H.G.

; Meotti, F.C.

; Miyamoto, S.

; Oliveira, C.C.

; Souza-Pinto, N.C.

; Reis, E.M.

; Ronsein, G.E.

; Salinas, R.K.

; Schechtman, D.

; Schreier, S.

; Setubal, J.C.

; Sogayar, M.C.

; Souza, G.M.

; Terra, W.R.

; Truzzi, D.R.

; Ulrich, H.

; Verjovski-Almeida, S.

; Winck, F.V.

; Zingales, B.

; Kowaltowski, A.J.

Brazilian Journal of Medical and Biological Research

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2019, Volume 52 N. 9 elocation e8935

Abstract: En | Text: En | PDF: En

The scientific publication landscape is changing quickly, with an enormous increase in options and models. Articles can be published in a complex variety of journals that differ in their presentation format (online-only or in-print), editorial organizations that maintain them (commercial and/or society-based), editorial handling (academic or professional editors), editorial board composition (academic or professional), payment options to cover editorial costs (open access or pay-to-read), indexation, visibility, branding, and other aspects. Additionally, online submissions of non-revised versions of manuscripts prior to seeking publication in a peer-reviewed journal (a practice known as pre-printing) are a growing trend in biological sciences. In this changing landscape, researchers in biochemistry and molecular biology must re-think their priorities in terms of scientific output dissemination. The evaluation processes and institutional funding for scientific publications should also be revised accordingly. This article presents the results of discussions within the Department of Biochemistry, University of São Paulo, on this subject.

https://doi.org/10.1590/1414-431x20198935 1769 downloads

Chemical Composition and Antibacterial Activity of the Essential Oil of Vitex agnus-castus L. (Lamiaceae)
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GONÇALVES, REGIANE

; AYRES, VANESSA F.S.

; CARVALHO, CARLOS E.

; SOUZA, MARIA G.M.

; GUIMARÃES, ANDERSON C.

; CORRÊA, GEONE M.

; MARTINS, CARLOS H.G.

; TAKEARA, RENATA

; SILVA, ELIANE O.

; CROTTI, ANTÔNIO E.M.

Anais da Academia Brasileira de Ciências

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dec 2017, Volume 89 N. 4 Pages 2825 - 2832

Abstract: En | Text: En | PDF: En

ABSTRACT Abnormal multiplication of oral bacteria causes dental caries and dental plaque. These diseases continue to be major public health concerns worldwide, mainly in developing countries. In this study, the chemical composition and antimicrobial activity of the essential oil of Vitex agnus-castus leaves (VAC‒EO) collected in the North of Brazil against a representative panel of cariogenic bacteria were investigated. The antimicrobial activity of VAC-EO was evaluated in terms of its minimum inhibitory concentration (MIC) values by using the broth microdilution method in 96-well microplates. The chemical constituents of VAC-EO were identified by gas chromatography (GC‒FID) and gas chromatography‒mass spectrometry (GC‒MS). VAC‒EO displayed some activity against all the investigated oral pathogens; MIC values ranged from 15.6 to 200 μg/mL. VAC-EO had promising activity against Streptococcus mutans (MIC= 15.6 μg/mL), Lactobacillus casei (MIC= 15.6 μg/mL), and Streptococcus mitis (MIC= 31.2 μg/mL). The compounds 1,8-cineole (23.8%), (E)-β-farnesene (14.6%), (E)-caryophyllene (12.5%), sabinene (11.4%), and α-terpinyl acetate (7.7%) were the major chemical constituents of VAC‒EO. VAC-EO displays antimicrobial activity against cariogenic bacteria. The efficacy of VAC-EO against S. mutans is noteworthy and should be further investigated.

https://doi.org/10.1590/0001-3765201720170428 2391 downloads

Evaluation of the efficacy and safety of endovascular management for transplant renal artery stenosis
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Valle, Leonardo G.M.

; Cavalcante, Rafael N.

; Motta-Leal-Filho, Joaquim M.

; Affonso, Breno B.

; Galastri, Francisco L.

; Doher, Marisa P.

; Guimarães-Souza, Nadia K.

; Cavalcanti, Ana K.N.

; Garcia, Rodrigo G.

; Pacheco-Silva, Álvaro

; Nasser, Felipe

Clinics

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dec 2017, Volume 72 N. 12 Pages 773 - 779

Abstract: En | Text: En | PDF: En

OBJECTIVES: To evaluate the safety and efficacy of endovascular intervention with angioplasty and stent placement in patients with transplant renal artery stenosis. METHODS: All patients diagnosed with transplant renal artery stenosis and graft dysfunction or resistant systemic hypertension who underwent endovascular treatment with stenting from February 2011 to April 2016 were included in this study. The primary endpoint was clinical success, and the secondary endpoints were technical success, complication rate and stent patency. RESULTS: Twenty-four patients with transplant renal artery stenosis underwent endovascular treatment, and three of them required reinterventions, resulting in a total of 27 procedures. The clinical success rate was 100%. All graft dysfunction patients showed decreased serum creatinine levels and improved estimated glomerular filtration rates and creatinine levels. Patients with high blood pressure also showed improved control of systemic blood pressure and decreased use of antihypertensive drugs. The technical success rate of the procedure was 97%. Primary patency and assisted primary patency rates at one year were 90.5% and 100%, respectively. The mean follow-up time of patients was 794.04 days after angioplasty. CONCLUSION: Angioplasty with stent placement for the treatment of transplant renal artery stenosis is a safe and effective technique with good results in both the short and long term.

https://doi.org/10.6061/clinics/2017(12)09 557 downloads

Utilização de plasma rico em plaquetas de coelhos com poucos leucócitos e hemácias para a consolidação de ossos
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Andrade, M.G.M.G.

; Rodrigues, G.M.

; Lima, D.P.

; Faria, L.M.

; Silva, L.V.P.

; Souza, R.R.

; Eurides, D.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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apr 2016, Volume 68 N. 2 Pages 276 - 282

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Objetivou-se utilizar o plasma rico em plaquetas, obtido por técnica capaz de produzir um produto autólogo, com reduzido número de leucócitos e hemácias, e avaliar, por meio de radiografias, a capacidade de cicatrização de uma falha óssea induzida no rádio de coelhos. Foram coletados 10,5mL de sangue por via intracardíaca, o qual foi distribuído em três tubos de hemossedimentação contendo citrato de sódio. Os tubos contendo as amostras foram submetidos a uma centrifugação a 2.000rpm (670,8G) por 20 minutos, e da coluna de sedimentação foram aspirados de cada tubo 1.000µL de plasma para a redução do volume sobrenadante. Aspirou-se o plasma acima do anel de leucócitos e transferiu-se para outro tubo para centrifugação a 2.000rpm por 10 minutos. O conteúdo plaquetário no fundo do tubo foi ressuspendido e homogeneizado a 1.000µL do plasma sobrenadante para formar o PRP líquido. Uma falha de 1,0cm foi realizada no rádio esquerdo de cada coelho. O tempo de consolidação foi observado por meio de exame radiográfico aos 45 e 90 dias de pós-operatório (PO). As imagens foram comparadas entre o grupo controle (G1) e o grupo que recebeu o implante de PRP por via transdérmica (G2). A evolução da cicatrização foi verificada com auxílio do programa Adobe Photoshop CC e em uma escala de cinza que representa o contraste. No G2 foi verificada média de 63% de contraste aos 45 dias de PO e de 65% aos 90 dias de PO. No G2, aos 45 dias de PO, a média encontrada foi de 42,7%, e aos 90 dias de PO, 31,25%, indicando que houve evolução do processo de reparação óssea em comparação ao grupo que não recebeu o implante de PRP. O método de obtenção de plasma rico em plaqueta autólogo de coelhos reduziu o número de leucócitos e hemácias e recuperou o número de plaquetas, sendo este maior ou igual aos valores fisiológicos para a espécie. O PRP obtido foi capaz de acelerar o processo de consolidação óssea em coelhos.

The aim of this study was to use the platelet-rich plasma obtained by a technique capable of producing an autologous product, with a reduced number of white blood cells and red blood cells and assessed by radiographs, the healing ability of a bone defect induced in the radio rabbits. 10.5mL of blood was collected via intracardiac blood sedimentation and distributed into three tubes containing sodium citrate. The tubes containing the samples were subjected to centrifugation at 2,000 rpm (670.8G) for 20 minutes and the sediment column were aspirated from each plasma tube1,000μL to reduce the supernatant volume. The plasma was aspirated from above the leukocytes ring and transferred to another tube for centrifugation at 2,000 rpm for 10 minutes. The platelet content in the tube bottom was resuspended and homogenized to 1,000μL plasma supernatant PRP to form the liquid. A1.0 cm failure was performed on the left radio of each rabbit. The healing time was observed by means of radiographic examination at 45 and 90 days after surgery. The images were compared between the control group (G1) and the group receiving PRP implant transdermally (G2). The healing progress was assessed with the help of Adobe Photoshop program and a gray scale that represents the contrast. G2 had an average of 63% contrast at 45 days PO and 65% at 90 days PO. In G2, at 45 days PO the average was 42.7% and at 90 days PO 31.25% indicating that there was an increase in bone repair process compared to the group that did not receive the PRP implant. The method of obtaining an autologous platelet rich plasma of rabbits reduced the number of leukocytes and erythrocytes and platelets recovered which is greater than or equal to the physiological range for the species. The obtained PRP was able to accelerate the process of bone healing in rabbits.

https://doi.org/10.1590/1678-4162-8304 2034 downloads

Aspectos clínicos, cirúrgicos, histológicos e pós-operatórios de oito cadelas com leiomioma vaginal
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Menegassi, C.C.

; Martins, I.C.S.

; Pereira, G.M.

; Gomes, L.G.

; Bezerra, K.S.

; Spiller, P.R.

; Martini, A.C.

; Souza, R.L.

; Ribeiro, A.P.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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apr 2016, Volume 68 N. 2 Pages 307 - 312

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O presente trabalho reporta as características clínicas e cirúrgicas de oito casos de cadelas portadoras de leiomioma vaginal, com histórico de aumento de volume na região perineal, aparecimento de massa pela vulva, disquezia, tenesmo e disúria. As pacientes foram submetidas à cirurgia de episiotomia para ressecção da massa neoplásica, e em quatro casos foi necessária a ovariossalpingo-histerectomia. Porém, uma delas apresentou recidiva, e, no segundo procedimento cirúrgico de exérese de neoformação, o histopatológico diagnosticou leiomiossarcoma. A paciente ainda apresentou mais duas recidivas, até o proprietário optar pela eutanásia. Decorridos quatro meses, as sete cadelas diagnosticadas com leiomioma não apresentaram recidiva, e os proprietários relataram ausência de sinais relacionados com a doença descrita. Conclui-se que a episiotomia para ressecção da massa vaginal e a ovariossalpingo-histerectomia nas cadelas inteiras nos casos de tumores vaginais benignos são tratamentos satisfatórios, com bom prognóstico, ao contrário do leiomiossarcoma.

This study reports the clinical and surgical procedures of eight cases of bitches with vaginal leiomyoma. All animals presented increased volume in the perineal region, and exteriorization of the vulva, dyschezia, tenesmus and dysuria. The patients underwent episiotomy surgery for resection of the neoplastic mass, and ovariosalpingohisterectomy in four cases. However, one had recurrence on the second surgical procedure for removal of neoformation, histopathological diagnosed leiomyosarcoma, the patient also had two more relapses, until the owner chose euthanasia. After four months, the seven dogs diagnosed with leiomyoma showed no recurrence and the owners reported no signs of the disease described. We concluded that episiotomy for vaginal mass resection combined with ovariohysterectomy in cases of benign vaginal tumors have shown satisfactory treatment with good prognostic.

https://doi.org/10.1590/1678-4162-8232 7953 downloads

10.

Influência de diferentes tipos de micro-organismos na contagem bacteriana total por citometria de fluxo do leite cru refrigerado
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Sampaio, V.S.C.

; Souza, F.N.

; Saraiva, C.J.C.

; Santos, R.P.

; Leite, M.O.

; Resende, G.M.

; Gonçalves, N.C.

; Dionizio, F.L.

; Cerqueira, M.M.O.P.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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apr 2015, Volume 67 N. 2 Pages 607 - 612

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Contagens bacterianas de 179 amostras de leite cru refrigerado, provenientes de diferentes bacias leiteiras de Minas Gerais, foram realizadas pelos métodos de citometria de fluxo e contagem padrão em placas. O estudo das associações entre a contagem bacteriana total e as contagens bacterianas dos diferentes grupos de micro-organismos foi realizado pela correlação de Pearson. Houve uma forte correlação positiva e significativa entre os valores de contagem de psicrotróficos (r = 0,67) e mesófilos (r = 0,70), determinados pelo método de referência, e os valores da contagem bacteriana total, determinados pela citometria de fluxo. Foi possível estabelecer uma curva de calibração para o citômetro de fluxo utilizando equações de regressão linear. As equações para a contagem bacteriana obtida por citometria de fluxo em função da contagem de mesófilos e psicrotróficos foram: log (CBI) = 0,701 log mesófilos + 1,9091 e log (CBI) = 0,4703 log psicrotróficos + 3,2784, respectivamente. Não foi possível estabelecer curvas de calibrações para termófilos e termodúricos, pois as correlações obtidas entre as contagens desses micro-organismos e a contagem bacteriana total, determinada por citometria de fluxo, foram fracas (r = 0,30 e 0,27, respectivamente).

Total Bacterial count was performed in 179 raw milk samples from different dairy farms in Minas Gerais State, Brazil, through flow cytometry and standard plate count, that was regarded as the reference method. There was significant strong correlation between the values of psychrotrophic and mesophilic counts determined by the reference method with the values of bacterial count determined by flow cytometry (r = 0.67 and 0.70, respectively), which allowed to establish linear regression equations for bacterial count to calibrate the flow cytometry and estimate the count of mesophilic (log bacterial individual count (BIC) by flow cytometry = 0.701 log mesophilic + 1.9091) and psychrotrophic (log BIC by flow cytometry = 0.4703 log psychrotrophic + 3.2784) bacteria. It was not possible to establish calibration curves for thermophiles and thermodurics due to the weak to moderate correlation coefficients found among these microorganism counts and total bacterial count determined by flow cytometry (r = 0.30 and 0.27, respectively).

https://doi.org/10.1590/1678-7257 3069 downloads

11.

Determinação de parâmetros farmacognósticos para as folhas de Erythroxylum suberosum A. St.-Hilaire (Erythroxylaceae) coletadas no município de Goiânia, GO.
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RODRIGUES, G.A.

; SOUZA, W.C.

; GODINHO, M.G.C.

; FERREIRA, H.D.

; VILA VERDE, G.M.

Revista Brasileira de Plantas Medicinais

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2015, Volume 17 N. 4 Pages 1169 - 1176

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO As plantas medicinais representam um dos principais recursos terapêuticos utilizado pelo homem para a cura e prevenção de doenças, no entanto, para que o tratamento seja seguro e eficaz é necessário conhecer seus componentes químicos. Dessa forma, o intuito deste trabalho foi determinar os parâmetros farmacognosticos para o estabelecimento do controle de qualidade da droga vegetal composta pelas folhas de Erythroxylum suberosum A. St.-Hil., pertencentes à família Erythroxylaceae ocorrente no Cerrado. Para tanto, foram coletadas folhas adultas nas proximidades da Universidade Federal de Goiás, Goiânia-GO. Foram determinados o teor de umidade por meio de balança com irradiação de raios infravermelho e o teor de cinzas totais. Na prospecção fitoquímica foram pesquisadas as classes de metabólitos secundários e realizou-se o doseamento de fenóis totais, flavonoides e taninos totais. Os resultados dos testes de pureza realizados, teor de umidade e teor de cinzas totais, estão de acordo com os limites estabelecidos pelas especificações farmacopeicas. As análises fitoquímicas evidenciaram a presença de flavonoides, taninos, cumarinas, saponinas e resinas. No doseamento de fenóis totais, taninos e flavonoides presentes nas folhas de E. suberosum A. St.-Hil. obteve-se respectivamente 17,97%, 6,31%, 3,87%. Estes resultados confirmam os dados da literatura quanto à presença destes compostos em Erythroxylaceae, pois de acordo com nas folhas de E. tortuosum obteve-se valores de 10%, 8,4% e 0,064% de Fenóis, Taninos e flavonoides, respectivamente e nas folhas de E. deciduum foram encontrados 12,04% de fenóis totais, 0,87% de taninos e 1,37% de flavonoides. Os resultados encontrados no presente trabalho também se tornam relevantes quando comparadas as quantidades desses metabólitos com espécies clássicas na biossíntese de compostos fenólicos como o teor de taninos de Stryphnodendron adstringens (Mart.) Coville e em Eugenia uniflora L. foi de 29,9% e 2,96%, respectivamente. Teores de flavonóides que variaram entre 0,65% a 0,79% na Calendula officinalis L. e Ginkgo bilobaL. 0,59%, 0,75% e 0,79%. Sendo assim, a quantidade considerável de compostos fenólicos obtida em E. suberosum A. St.-Hil. sugere que a espécie possua um importante potencial terapêutico e quem sabe potencial antioxidante, a ser explorado em estudos posteriores.

ABSTRACT Medicinal plants represent a major therapeutic resources used by man for the cure and prevention of diseases, however, that the treatment be safe and effective it is necessary to know their chemical components. Thus, the aim of this work was to determine the pharmacognostic parameters for the establishment of the plant drug quality control made by the leaves of Erythroxylum suberosum A. St.-Hil., belonging to the family Erythroxylaceae occurring in the Cerrado. To this end, adult leaves were collected near the Federal University of Goiás, Goiânia-GO. It was determined the moisture content through scale radiating infrared rays and the content of total ash. The phytochemical screening of secondary metabolites classes were surveyed and performed the determination of total phenols, flavonoids and total tannins. The results of the purity tests, moisture content and total ash content, are in accordance with the limits established by the pharmacopeic specifications. The phytochemical analysis revealed the presence of flavonoids, tannins, coumarins, saponins and resins. The determination of total phenols, tannins and flavonoids quantities present in the leaves of E. suberosum A. St.-Hil. were obtained respectively as 17.97%, 6.31%, 3.87%. These results confirm the literature data for the presence of these compounds in Erythroxylaceae, since according to the leaves of E. tortuosum yielded values of 10%, 8.4% and 0.064% of phenols, tannins and flavonoids, respectively. On the E. deciduum leaves were found 12.04% of total phenols, tannins 0.87% and 1.37% of flavonoids. The results of this work also become relevant when comparing the amounts of these metabolites with classic species in the biosynthesis of phenolic compounds such as tannins content in barbatimão and pitanga was 29.9% and 2.96%, respectively. Flavonoid contents ranging from 0.65% to 0.79% in calendula and ginkgo 0.59%, 0.75% and 0.79%. Thus, considerable amount of phenolic compounds obtained in E. suberosum. A. St. Hil. It suggests that the species has an important therapeutic potential and who knows antioxidant potential to be explored in further studies.

https://doi.org/10.1590/1983-084x/14_114 5462 downloads

12.

Desempenho de frangos de corte alimentados com altos valores de balanço eletrolítico em região de clima quente
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Vieites, F.M.

; Fraga, A.L.

; Souza, C.S.

; Araújo, G.M.

; Vargas Júnior, J.G.

; Nunes, R.V.

; Corrêa, G.S.S.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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apr 2011, Volume 63 N. 2 Pages 441 - 447

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Determinaram-se os melhores valores de balanço eletrolítico (BE) para o desempenho de frangos de corte de um a sete, um a 14, um a 21 e um a 42 dias de idade. Utilizaram-se 720 pintos comerciais, machos, criados em galpão de alvenaria, em boxes cobertos com maravalha, alimentados com dieta basal composta por milho e farelo de soja combinada com valores de BE de 200; 250; 300; 350 e 400mEq/kg. Adotou-se o delineamento inteiramente ao acaso, com seis repetições de 24 aves para cada uma das 30 unidades experimentais. Avaliaram-se o ganho de peso, o consumo da dieta e a conversão alimentar aos sete, 14, 21 e 42 dias de idade. O melhor BE estimado para o ganho de peso aos 21 e 42 dias foi de 270mEq/kg, estimado pelo modelo quadrático de regressão. Para as demais variáveis, não houve diferenças entre os BE. Recomenda-se um valor de BE entre 270 e 300mEq/kG para frangos de corte para as fases inicial e de crescimento em regiões de clima quente.

A trial was carried out to determine the best electrolyte balance value (EB) for broilers performance from 1 to 7, 1 to 14, 1 to 21 and 1 to 42 days of age. A total of 720 one-day-old chicks, males, were reared in the floor covered with shaving woods and fed corn-soybean base diets combined with 200; 250; 300; 350 e 400mEq/kg of EB. A completely randomized experimental design with six replicates of 24 chicks each and 36 experimental units was used. Weigh gain (WG), feed intake (FC) and feed efficiency (FE) were evaluated at 7, 14, 21 and 42 days of age. The best values of EB estimated for the WG at 21 and 42 days was 70mEq/kg, estimated by a quadratic regression model. No differences among treatments were observed for the other studied traits. The results suggested EB should be around 270 up to 300mEq/kg in high temperature regions, for broiler chicks in inicial and growth phases.

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13.

Effects of strain and age on ear wound healing and regeneration in mice
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Costa, R.A.

; Ruiz-de-Souza, V.

; Azevedo Jr., G.M.

; Vaz, N.M.

; Carvalho, C.R.

Brazilian Journal of Medical and Biological Research

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dec 2009, Volume 42 N. 12 Pages 1143 - 1149

Abstract: En | Text: En | PDF: En

Round holes in the ears of MRL mice tend to close with characteristics of regeneration believed to be absent in other mouse strains (e.g., C57BL/6). We evaluated the kinetics and the histopathology of ear wound closure in young (8 weeks old) C57BL/6 and BALB/c mice. We also used middle-aged (40 weeks old) C57BL/6 mice to evaluate the influence of aging on this process. A circular through-and-through hole was made in the ear, photographs were taken at different times after injury and wound area was measured with digital analysis software. The percentages of closed area measured on day 100 were: 23.57 ± 8.66% for young BALB/c mice, 56.47 ± 7.39% for young C57BL/6 mice, and 75.31 ± 23.65% for middle-aged C57BL/6 mice. Mice were sacrificed on days 1, 3, 5, 25, 44, and 100 for histological evaluation with hematoxylin and eosin, Gomori’s trichrome, periodic acid-Schiff, or picrosirius red staining. In young mice of both strains, healing included re-epithelialization, chondrogenesis, myogenesis, and collagen deposition. Young C57BL/6 and BALB/c mice differed in the organization of collagen fibers visualized using picrosirius-polarization. Sebaceous glands and hair follicles regenerated and chondrogenesis was greater in young C57BL/6 mice. In middle-aged C57BL/6 mice all aspects of regeneration were depressed. The characteristics of regeneration were present during ear wound healing in both young BALB/c and young C57BL/6 mice although they differed in intensity and pattern. Greater ear wound closure in middle-aged C57BL/6 mice was not correlated with regeneration.

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14.

Confusão sexual para o controle de Grapholita molesta (Lepidoptera: Tortricidae), em pomares de macieira, em Fraiburgo (SC), Brasil
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Monteiro, Lino B.

; Souza, Alexandre de

; Belli, Luiz

Bragantia

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2008, Volume 67 N. 1 Pages 191 - 196

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O controle de Grapholita molesta (Lepidoptera: Tortricidae) foi avaliado pela técnica de confusão sexual em macieira no município de Fraiburgo (SC). A formulação do feromônio sintético utilizada foi da marca Quant G.m. (400 mg do acetato de (Z/E) - 8 - dodecenila 14% p/v), com 500 difusores por hectare, distribuídos em um pomar de macieira comercial 'Fuji' de 2,52 ha. O tratamento com confusão sexual foi comparado com a testemunha convencional. O monitoramento para moscas-das-frutas, Anastrepha fraterculus (Diptera: Tephritidae); mariposa oriental, Grapholita molesta e lagarta enroladeira, Bonagota cranaodes (Lepidoptera: Tortricidae) foi realizado para decidir se haveria necessidade de pulverização de inseticidas. Não houve capturas de G. molesta na armadilha do Tipo Delta, instalada no tratamento com confusão sexual até 93 dias após a liberação do feromônio. Nesse período, realizaram-se duas pulverizações para moscas-das-frutas e lagarta-enroladeira no tratamento com confusão sexual e oito pulverizações no tratamento convencional. Os danos causados por G. molesta em ambos os tratamentos não ultrapassaram 0,5%, mesmo com a redução de 77% dos inseticidas no tratamento com confusão sexual. Verifica-se, portanto, que a técnica de confusão sexual é eficiente para o controle de G. molesta.

The control of Grapholita molesta (Lepidoptera: Tortricidae) was assessed by using the mating disruption technique in an apple orchard in Fraiburgo, SC. Quant G.m. synthetic pheromone formulation (400 mg of (Z/E) - 8 - dodecenyl acetate 14% p/v) was used, with 500 dispensers per hectare, distributed over a 2.52 hectare 'Fuji' commercial orchard. The mating disruption treatment was compared with a chemical control treatment. The action threshold was determined by monitoring fruit flies Anastrepha fraterculus (Diptera: Tephritidae), G. molesta and leafroller Bonagota cranaodes (Lepidoptera: Tortricidae). G. molesta was caught in the Delta traps installed in the mating disruption plot until 93 days from release of the pheromone. In this period, the mating disruption treatment was sprayed twice for fruit flies and leafrollers, and the chemical control plot received eight pesticides application. The damage caused by G. molesta in both treatments did not exceed 0.5%, even with a 77% reduction of insecticide in the mating disruption plot. We concluded that the mating disruption technique was efficient in the control of G. molesta.

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15.

Expression, purification and DNA-binding activities of two putative ModE proteins of Herbaspirillum seropedicae (Burkholderiales, Oxalobacteraceae)
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Souza, André L.F.

; Chubatsu, Leda S.

; Souza, Emanuel M.

; Pedrosa, Fábio O.

; Monteiro, Rose A.

; Rego, Fabiane G.M.

; Rigo, Liu U.

Genetics and Molecular Biology

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2008, Volume 31 N. 3 Pages 743 - 750

Abstract: En | Text: En | PDF: En

In prokaryotes molybdenum is taken up by a high-affinity ABC-type transporter system encoded by the modABC genes. The endophyte β-Proteobacterium Herbaspirillum seropedicae has two modABC gene clusters and two genes encoding putative Mo-dependent regulator proteins (ModE1 and ModE2). Analysis of the amino acid sequence of the ModE1 protein of H. seropedicae revealed the presence of an N-terminal domain containing a DNA-binding helix-turn-helix motif (HTH) and a C-terminal domain with a molybdate-binding motif. The second putative regulator protein, ModE2, contains only the helix-turn-helix motif, similar to that observed in some sequenced genomes. We cloned the modE1 (810 bp) and modE2 (372 bp) genes and expressed them in Escherichia coli as His-tagged fusion proteins, which we subsequently purified. The over-expressed recombinant His-ModE1 was insoluble and was purified after solubilization with urea and then on-column refolded during affinity chromatography. The His-ModE2 was expressed as a soluble protein and purified by affinity chromatography. These purified proteins were analyzed by DNA band-shift assays using the modA2 promoter region as probe. Our results indicate that His-ModE1 and His-ModE2 are able to bind to the modA2 promoter region, suggesting that both proteins may play a role in the regulation of molybdenum uptake and metabolism in H. seropedicae.

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