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There is a high demand for stroke rehabilitation in the Brazilian public health system, but most studies that have addressed rehabilitation for unilateral spatial neglect (USN) after stroke have been performed in high-income countries. Therefore, the aim of this study was to analyze USN patient recruitment in a multicenter noninvasive brain stimulation clinical trial performed in Brazil and to provide study design recommendations for future studies. We evaluated the reasons for exclusion of patients from a multicenter, randomized, double-blinded clinical trial of rehabilitation of USN patients after stroke. Clinical and demographic variables were compared between the included and excluded patients. A descriptive statistical analysis was performed. Only 173 of the 1953 potential neglect patients (8.8%) passed the initial screening. After screening evaluation, 87/173 patients (50.3%) were excluded for clinical reasons. Cognitive impairment led to the exclusion of 21/87 patients (24.1%). Low socioeconomic status led to the exclusion of 37/173 patients (21.4%). Difficulty obtaining transportation to access treatment was the most common reason for their exclusion (16/37 patients, 43.3%). The analyzed Brazilian institutions have potential for conducting studies of USN. The recruitment of stroke survivors with USN was restricted by the study design and limited financial support. A history of cognitive impairment, intracranial stenting or craniectomy, and lack of transportation were the most common barriers to participating in a multicenter noninvasive brain stimulation trial among patients with USN after stroke.

https://doi.org/10.1590/1414-431x2023e12326

Evaluation of biochemical and electrolytic components of semen from ram supplemented with different concentrations of selenium and its correlation with sperm quality
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Silva, D.C.

; Codognoto, V.M.

; Piagentini, M.

; Dantas, A.

; Sousa, G.C.

; Silva, L.S.

; Souza, E.R.

; Almeida Filho, R.A.

; Denadai, R.

; Oba, E.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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dec 2022, Volume 74 N. 6 Pages 1065 - 1071

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O objetivo do estudo foi avaliar se a suplementação com diferentes níveis de selênio (Se) pode alterar os componentes bioquímicos e eletrolíticos do sêmen, ocasionando, por conseguinte, uma melhoria na qualidade seminal em carneiros. Foram utilizados 30 animais, mantidos em sistema intensivo de baias, alimentados com feno e ração comercial, sendo alocados em cinco grupos (seis animais/baia) e suplementados com uma mistura mineral contendo 0mg (G1), 5mg (G2), 10mg (G3), 15mg (G4) e 20mg (G5) de Se/kg. Cada grupo recebeu um tratamento diferente a cada 56 dias, e foi realizado um rodízio de tratamentos entre os grupos seguindo uma sequência dinâmica. Amostras de sêmen foram colhidas por eletroejaculação após o fim de cada tratamento, a fim de se avaliarem os níveis de frutose, ácido cítrico, potássio (K), sódio (Na), cálcio (Ca), Se, zinco (Zn), manganês (Mn), enxofre (S) e chumbo (Pb). O desenho estatístico foi um quadrado latino 5x5. Os diferentes níveis de suplementação de Se avaliados mantiveram as concentrações de eletrólitos e de minerais do sêmen nos níveis exigidos e não modificaram a qualidade espermática, concluindo-se que maiores ingestões de Se não causam efeitos antagônicos na absorção e subsequente ação de outros minerais essenciais fornecidos aos animais e ainda mantêm o equilíbrio eletrolítico.

ABSTRACT The aim of the study was to evaluate whether supplementation with different levels of selenium (Se) can change the biochemical and electrolytic components of semen, causing an improvement in seminal quality in rams. Thirty animals were kept in an intensive pen system, fed with hay and commercial ration, allocated into five groups (six animals/pen) and supplemented with a mineral mixture containing 0mg (G1), 5mg (G2), 10mg (G3), 15mg (G4) and 20mg (G5) of Se/kg. Each group received a different treatment every 56 days and treatments were rotated between groups following a dynamic sequence. Semen samples were collected by electroejaculation after the end of each treatment to evaluate the levels of fructose, citric acid, potassium (K), sodium (Na), calcium (Ca), Se, zinc (Zn), manganese (Mn), sulfur (S) and lead (Pb). The statistical design was a 5x5 Latin square. The different levels of Se supplementation evaluated maintained the concentrations of electrolytes and minerals in the semen at the required levels and did not change the sperm quality, concluding that higher intakes of Se do not cause antagonistic effects on the absorption and subsequent action of other essential minerals supplied to the animals and still maintains electrolyte balance.

https://doi.org/10.1590/1678-4162-12690

Morfometria e população de folículos pré-antrais de vacas Nelore vazias, gestantes e de fetos nos terços inicial, médio e final da gestação
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Gonçalves, C.G.P.

; Tironi, S.M.T.

; Silva, K.M.

; Silva Junior, E.R.

; Codognoto, V.M.

; Souza, A.G.

; Marques, N.F.S.

; Brochine, S.

; Vieira, A.F.

; Oba, E.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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oct 2019, Volume 71 N. 5 Pages 1735 - 1740

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

ABSTRACT The aim of this study was to describe population and morphological characteristics of preantral follicles of not pregnant cows, pregnant cows and fetus. Ten ovaries of non-pregnant Nelore cows, eighteen ovaries of pregnant cows and eighteen ovaries of fetus were used. For pregnant cows, six ovaries from each third (initial, middle and final) were evaluated, acquired from a slaughterhouse. For fetus, the same methodology and proportion of ovaries were used. Ovaries were washed, fixed and embedded in paraffin. They were then sectioned in longitudinal sections and stained by the Hematoxylin-Eosin method. Preantral follicles were classified according to morphology (primordial, primary and secondary) and degree of viability (intact and in initial, moderate and marked atresia). Descriptive and statistical analyzes were performed through KS300 image analysis program and Tukey’s test. A greater proportion of primordial follicles were found in all categories. Secondary follicles were not observed in ovaries of fetus and cows in the initial third of pregnancy. All the ovary dimensions were higher in non-pregnant cows and in the final third of cows' pregnancy, and lower in final third of pregnancy fetus. It was concluded that follicle isolation was effective in describing population and morphological characteristics of preantral follicles of cows and fetus.

https://doi.org/10.1590/1678-4162-11409 729 downloads

Germination of Stigmaphyllon blanchetii Seeds in Different Temperatures and Luminosity
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SILVA, E.M.

; PEREIRA, J.C.

; FERREIRA, V.M.

; SOUZA, R.C.

Planta Daninha

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2019, Volume 37 elocation e019197178

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: O presente trabalho teve por objetivo estudar a influência da temperatura e da luz sobre a germinação das sementes de Stigmaphyllon blanchetii, conhecida popularmente como rabo-de-rato. Na primeira etapa da pesquisa, foi avaliado o efeito de três temperaturas constantes (20 oC, 25 oC e 30 oC) e uma alternada de 20-30 oC. Na segunda etapa, para simulação da luz sob diferentes qualidades espectrais, foram utilizadas quatro condições de luminosidade (luz branca, luz vermelha, vermelho distante e ausência de luz). As temperaturas que proporcionaram melhor germinação e desenvolvimento para S. blanchetii foram a constante de 30 oC e alternada de 20-30 oC. As sementes germinaram tanto na presença como na ausência de luz, porém houve maior germinação e desenvolvimento das plântulas na ausência de luz, podendo ser classificadas em fotoblásticas negativas preferenciais.

ABSTRACT: The objective of the present work was to study the influence of temperature and light on germination of seeds of Stigmaphyllon blanchetii, popularly known as rat tail. The first stage of the research evaluated the effect of three constant temperatures (20 oC, 25 oC and 30 oC) and an alternating temperature (20-30 oC). In the second stage, for simulation of different types of light, four conditions of luminosity (white, red, far red and absence of light) were used. The temperatures that provided the best germination and development for S. blanchetii are the constant (30 oC) and the alternating (20-30 oC) ones. The seeds germinated both in the presence and absence of light, but there was greater germination and seedling development in the absence of light; thus they were classified as preferential negative photoblastic seeds.

https://doi.org/10.1590/s0100-83582019370100120 795 downloads

Antiedematogenic activity of the indole derivative N-salicyloyltryptamine in animal models
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SOUSA-NETO, BENEDITO P.

; GOMES, BRUNO S.

; CUNHA, FRANCISCO V.M.

; ARCANJO, DANIEL D.R.

; GUTIERREZ, STANLEY J.C.

; SOUZA, MARIA F.V.

; ALMEIDA, FERNANDA R.C.

; OLIVEIRA, FRANCISCO A.

Anais da Academia Brasileira de Ciências

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mar 2018, Volume 90 N. 1 Pages 185 - 194

Abstract: En | Text: En | PDF: En

ABSTRACT The N-salicyloyltryptamine (NST) is an indole derivative compound analogue to the alkaloid N-benzoyltryptamine. In the present study, the antiedematogenic activity of NST was investigated in animal models. Firstly, the acute toxicity for NST was assessed according to the OECD Guideline no. 423. The potential NST-induced antiedematogenic activity was evaluated by carrageenan-induced paw edema in rats, as well as by dextran-, compound 48/80-, histamine-, serotonin-, capsaicine-, and prostaglandin E2-induced paw edema in mice. The effect of NST on compound 48/80-induced ex vivo mast cell degranulation on mice mesenteric bed was investigated. No death or alteration of behavioral parameters was observed after administration of NST (2000 mg/kg, i.p.) during the observation time of 14 days. The NST (100 and 200 mg/kg, i.p.) inhibited the carrageenan-induced edema from the 1st to the 5th hour (**p<0.01; ***p<0.001). The edematogenic activity induced by dextran, compound 48/80, histamine, serotonin, capsaicin, and prostaglandin E2 was inhibited by NST (100 mg/kg, i.p.) throughout the observation period (**p<0.01; ***p<0.001). The pretreatment with NST (50, 100 or 200 mg/kg, i.p) attenuates the compound 48/80-induced mast cell degranulation (**p<0.01; ***p<0.001). Thus, the inhibition of both mast cell degranulation and release of endogenous mediators are probably involved in the NST-induced antiedematogenic effect.

https://doi.org/10.1590/0001-3765201720160502 1340 downloads

Posibilidades en la educación en enfermería: errar, crear y arriesgar
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Souza, D.

; Schubert-Backes, V.M.

; Delacanal-Lazzari, D.

Enfermería universitaria

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jun 2017, Volume 14 N. 2 Pages 79 - 87

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

Resumo Introdução: O desenvolvimento docente é ininterrupto e a necessidade de contínuo aprendizado, por meio da formação docente é imprescindível para o fortalecimento da práxis, além da necessidade de uma avaliação crítico reflexiva na e sobre a prática docente. Objetivo: Identificar estratégias utilizadas para aperfeiçoar a prática docente dos professores enfermeiros. Método: Estudo qualitativo descritivo, com referencial teórico de Lee Shulman, realizado por meio de três oficinas pedagógicas, nos meses de abril e maio de 2013, com seis docentes de Enfermagem de educação profissional. Para análise dos dados, foi utilizada a análise temática com a proposta operativa de Minayo. Resultados: Da base de conhecimentos para o ensino de Shulman, aparece prioritariamente o Conhecimento do Conteúdo e o Conhecimento Pedagógico Geral, que fortalecem o Conhecimento Pedagógico do Conteúdo (CPC) dos docentes e os instrumentaliza para o momento do ensino. O CPC destes docentes tem suas fontes alimentadas por meio da reflexão (reflexão sobre os erros, acertos e feedback dos alunos), potencializadas pela dimensão pessoal e aproximação à disciplina lecionada, com domínio teórico. Conclusão: As particularidades e experiências docentes, que marcam as trajetórias pessoais, profissionais e acadêmicas dos docentes, influenciam o modo de ensino e determinam o desenvolvimento do processo de construção e ampliação das fontes do CPC. Há necessidade de participação em encontros de capacitação pedagógica para balizar o exercício docente e defende-se a necessidade e relevância de estudos do CPC na Enfermagem, por possibilitar a colaboração às pesquisas no ensino de enfermagem e saúde, fornecendo subsídios para estratégias de formação docente.

Abstract Introduction: The need of continuous learning development of teaching staff, as well as its related critical-reﬂexive assessment, is necessary to strengthening the praxis. Objective: To identify strategies utilized to improve the teaching of nurse professors. Method: This is a qualitative descriptive study with theoretical referencing to Lee Shulman, carried out through 3 pedagogic workshops, during April and May 2013, with 6 professional nursing educators. Data were thematically analyzed using Minayo operative proposal. Results: From Shulman’s teaching knowledge base, it appears the knowledge of content and general pedagogical knowledge which strengthen the content pedagogical knowledge (CPK) of professors and instrument them to the moment of teaching. The CPK of these teachers has its feeding source through reﬂection (reﬂection on errors, objectives and student’s feedback), potentiated by the personal dimension and the proximity with the discipline thought as a theoretical domain. Conclusion: The teaching particularities and experiences which mark the personal, professional, and academic trajectories of teachers inﬂuence the mode of teaching and determine the development of the process of construction and enhancement of the CPK source. There is a need of participation in meetings of pedagogic training to orient the teaching exercise, and defend the need and relevance of studies on CPK in nursing, to make possible the collaboration towards research in the teaching of nursing and health, providing bases for strategies of teaching formation.

Resumen Introducción: El desarrollo docente es ininterrumpido y la necesidad de aprendizaje continuo por medio de la formación docente es imprescindible para el fortalecimiento de la praxis, además de la necesidad de una evaluación crítico-reﬂexiva en, y sobre la práctica docente. Objetivo: Identificar estrategias utilizadas para perfeccionar la práctica docente de los profesores enfermeros. Método: Estudio cualitativo descriptivo, con referencial teórico de Lee Shulman, realizado por medio de tres talleres pedagógicos, en los meses de abril y mayo de 2013, con seis docentes de enfermería de educación profesional. Para el análisis de los datos fue utilizado el análisis temático con la propuesta operativa de Minayo. Resultados: De la base de conocimientos para la enseñanza de Shulman, aparece prioritariamente el conocimiento del contenido y el conocimiento didáctico general, que fortalecen el conocimiento didáctico del contenido (CDC) de los docentes y los instrumentaliza para el momento de la enseñanza. El CDC de estos docentes tiene su fuente de alimentación a través de la reﬂexión (reﬂexión sobre los errores, aciertos y retroalimentación de los alumnos), potencializada por la dimensión personal y la proximidad con la disciplina enseñada con dominio teórico. Conclusión: Las particularidades y experiencias docentes, que marcan las trayectorias personales, profesionales y académicas de los docentes, inﬂuencian el modo de enseñar y determinan el desarrollo del proceso de la construcción y ampliación de la fuente del CDC. Hay necesidad de participación en encuentros de capacitación pedagógica para orientar el ejercicio docente, y defender la necesidad y relevancia de estudios del CDC en la enfermería, a fin de posibilitar la colaboración hacia las investigaciones en la enseñanza de la enfermería y la salud, suministrando apoyos para estrategias de formación docente.

https://doi.org/10.1016/j.reu.2017.02.005 1519 downloads

Bases teóricas da reconexão magnética
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Souza, V.M.

; Silveira, M.V.D.

; Koga, D.

; Jauer, P.R.

Revista Brasileira de Ensino de Física

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jun 2016, Volume 38 N. 2 elocation e2301

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Neste trabalho, o conceito e a origem histórica do processo físico conhecido como reconexão magnética são apresentados, bem como um dos primeiros modelos analíticos que serviu como base teórica para futuras investigações do fenômeno. A reconexão pode ocorrer entre dois ou mais regimes de plasmas magnetizados próximos o suficiente para que efeitos magneto-hidrodinâmicos não ideais permitam a reestruturação topológica dos seus respectivos campos magnéticos. Como consequência, o plasma pode ser acelerado num curto espaço de tempo, tal como observado em fenômenos físicos explosivos como, por exemplo, as explosões (flares) solares. Focam-se nos primeiros esforços analíticos propostos para explicar o processo segundo a perspectiva da magneto-hidrodinâmica. A reconexão magnética tem o potencial de tornar-se um mecanismo universal o qual pode ser utilizado no auxílio ao entendimento de uma miríade de processos físicos ocorrendo tanto em plasmas de laboratório, como em plasmas astrofísicos.

In this work both the concept and historical origins of the physical process known as magnetic reconnection are presented, as well as one of the first analytical models which was used as theoretical basis for future investigations on the phenomenon. Magnetic reconnection can occur between two or more magnetized plasma regimes which are close enough to allow non-ideal magnetohydrodynamic effects to take place and consequently change the topological structure of the interacting magnetic fields. As a result, the plasma can be accelerated in a short amount of time, as has been observed in explosive physical phenomena like solar flares. In this work, it is emphasized the first mathematical efforts employed in order to understand and describe reconnection according to the point of view of magnetohydrodynamics. Magnetic reconnection has the potential of becoming a universal mechanism which can be used to help to understand a myriad of physical processes occurring in both laboratory and astrophysical plasmas.

https://doi.org/10.1590/S1806-11173812132 2462 downloads

Fatty acids intake and deposition in the Longissimus of Nellore steers supplemented at pasture
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Oliveira, A.P.

; Silva, R.R.

; Souza, S.O.

; Carvalho, G.G.P.

; Silva, F.F.

; Lisboa, M.M.

; Pereira, M.M.S.

; Carvalho, V.M.

; Mendes, F.B.L.

; Oliveira, A.C.

Archivos de Zootecnia

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mar 2014, Volume 63 N. 241 Pages 85 - 95

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

The aim of this work was to study the correlation between intake and deposition of fatty acids in the Longissimus muscle of Nellore steers supplemented at pasture. The fieldwork was implanted in an area of 52 ha, formed of Brachiaria brizantha. Forty Nellore steers with initial average weight of 373.7 ± 14.9 kg and 26 months of age were used. The animals were distributed into four treatments: Control= T00 (supply only mineral salt), T03= Supply of concentrate in the amount of 0.3 % of Body Weight; T06= Supply of concentrate in the amount of 0.6 % of Live Weight , T09= Supply of concentrate in the amount of 0.9 % of live weight. Samples of concentrates and forages were collected for fatty acids analysis. The experiment lasted for 98 days. After this period the animals were slaughtered. Longissimus samples were vacuum packed and frozen for analysis of fatty acid composition. The results were statistically analyzed by estimating Pearson linear correlation coefficients. The results showed that there are correlations between the fatty acids consumed and fatty acids deposited in the muscle.

Objetivou-se estudar a correlação entre consumo e deposição de ácidos graxos no músculo Longissimus de novilhos nelore suplementados em pastagens. O trabalho de campo foi implantado numa área de 52 ha, formada de Brachiaria brizantha. Foram utilizados 40 novilhos da raça Nelore com peso inicial médio de 373,7 ± 14,9 kg e 26 meses de idade, distribuídos em quatro tratamentos: Controle= T00 (fornecimento apenas de sal mineral), T03= fornecimento de 0,3% concentrado em função do peso vivo; T06= fornecimento de 0,6 % concentrado em função do peso vivo; T09= fornecimento de 0,9 % concentrado em função do peso vivo. Amostras dos concentrados e da forragem foram coletadas para análise da composição de ácidos graxos. O período experimental foi de 98 dias. Após este período, os animais foram abatidos. Os resultados foram analisados por correlação linear de Pearson e teste t. Constatou-se que existem correlações entre os ácidos graxos consumidos e depositados no músculo.

https://doi.org/10.4321/S0004-05922014000100009 362 downloads

Degradabilidade in situ das silagens de variedades de cana-de açúcar com aditivos
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Silva, G.W.V.

; Rocha Júnior, V.R.

; Rocha, W.J.B.

; Reis, S.T.

; Pires, D.A.A.

; Antunes, A.P.S.

; Almeida Filho, S.H.C.

; Oliveira, L.M.

; Caldeira, L.A.

; Souza, V.M. de

Archivos de Zootecnia

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mar 2014, Volume 63 N. 241 Pages 171 - 182

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

The aim of this work was to evaluate the in situ degradability of the dry matter, crude protein and neutral detergent fiber of two sugarcane varieties ensiled with additives. The evaluation of the degradability was carried out according to a random blocks design, in factorial scheme 2 x 6, being two cane varieties (IAC 86-2480 and RB 86-7515), and five additives (urea, NaOH, CaO, corn and Lactobacillus buchneri), plus the control silage, with 3 blocks (animals). The NaOH and the CaO increased the values of soluble fraction and the effective degradability of dry matter. The soluble fraction and the effective degradability of the crude protein were higher with urea. The effective degradability of the neutral detergent fiber of the two varieties was higher in the silages with NaOH and CaO. The silages treated with NaOH and CaO are the best in nutritional value, because of the highest effective degradability of dry matter and neutral detergent fiber. The IAC 86-2480 variety presents better nutritional value than RB 86-7515, due to the highest effective degradability of dry matter.

Objetivou-se com este trabalho avaliar a degradabilidade in situ da matéria seca, proteína bruta e fibra em detergente neutro das silagens de variedades de cana-de-açúcar com aditivos. A avaliação da degradabilidade foi realizada segundo um delineamento em blocs casualizados, em esquema fatorial 2 x 6 sendo duas variedades de cana (IAC 86-2480 e RB 86-7515), e cinco aditivos (ureia, NaOH, CaO, milho grão moído e Lactobacillus buchneri), mais a silagem-controle, com 3 blocs (animais). A fração solúvel e a degradabilidade efetiva da matéria seca das silagens com NaOH e CaO aumentaram. A fração solúvel e a degradabilidade efetiva da proteína bruta foram maiores nas silagens com ureia. A degradabilidade efetiva da fibra em detergente neutro das duas variedades foi maior nas silagens com NaOH e CaO. As silagens com NaOH e CaO são de melhor valor nutricional, pois apresentam maior degradabilidade efetiva da matéria seca e fibra em detergente neutro. A variedade IAC 86-2480 apresenta melhor valor nutricional em relação à RB 86-7515, devido à maior degradabilidade efetiva da matéria seca.

https://doi.org/10.4321/S0004-05922014000100017 416 downloads

10.

Toxicidade do pericarpo da Jatropha curcas em ovinos
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Ferreira, O.R.

; Brito, S.S.

; Lima, F.G

; Souza, D.P. Mariano

; Mendonça, S.

; Ribeiro, J.A.A.

; Maiorka, P.C.

; Araújo, V.L

; Neiva, J.N.M

; Fioravante, M.C.S.

; Ramos, A.T.

; Maruo, V.M.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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jun 2012, Volume 64 N. 3 Pages 559 - 567

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O pinhão manso (Jatropha curcas) é uma planta cultivada para a produção de biocombustível. O pericarpo é um coproduto com potencial para alimentação animal, e a presença de componentes tóxicos, principalmente ésteres de forbol, pode limitar sua utilização. Assim, objetivou-se avaliar a toxicidade do pericarpo. Vinte ovinos foram distribuídos em quatro grupos - um grupo-controle, que não recebeu a planta, e três experimentais, que receberam o pericarpo nas concentrações de 15% (G15), 30% (G30) e 45% (G45), durante 23 dias. Após o 10º dia, a ingestão do pericarpo promoveu redução do consumo de alimento, diarreia, desidratação e caquexia. Todos os grupos tratados apresentaram redução na concentração de fosfatase alcalina. Animais do G30 apresentaram redução na concentração de ureia e proteínas totais e elevação de potássio e sódio. No G45, houve aumento de aspartato aminotransferase, albumina, creatinina bilirrubina indireta e total. A avaliação anatomo-histopatológica revelou ascite, hidropericárdio, congestão no trato gastrintestinal e nos pulmões, edema pulmonar, aderências à parede torácica, degeneração hepática centrolobular e das células tubulares renais, pneumonia linfo-histiocitica e enterite linfoplasmocitária e histiocítica. À análise fitoquímica, constatou-se 0,3845mg de ésteres de forbol/g de pericarpo. Conclui-se que o pericarpo de J. curcas é tóxico, não sendo recomendado para alimentação de ovinos.

Physic nut (Jatropha curcas) is a plant cultivated for biofuel production. Pericarp is a potential livestock food source by-product. However, its use may be limited due to the presence of toxic compounds, mainly phorbol esters. Thus, this study aimed to evaluate pericarp toxicity. Twenty sheep were divided in four groups, one control group which did not receive the plant and three experimental groups which received pericarp in 15% (G15), 30% (G30) and 45% (G45) concentrations for 23 days. After 10 days of treatment, pericarp ingestion produced food intake decrease, diarrhea, dehydration and loss of body condition. All treated groups showed decrease in alkaline phosphatase activity. G30 animals presented reductions in urea and total protein concentrations, and increase in potassium and sodium levels. G45 animals showed increase in serum aspartate aminotransferase activity and in albumin, creatinin, total and indirect bilirubin levels. Anatomohistopathologic findings included ascites, hydropericardium, congestion of the gastintestinal tract and lungs, pulmonary edema and adhesions in the thoracic cavity, renal tubular cells and centrilobular cytoplasmic vacuolation and lymphohistiocytic pneumonia and lymphoplasmacytic and histiocytic enteritis. On the physiochemical analysis 0.3845mg of phorbol esters/g of pericarp were detected. It is concluded that J. curcas pericarp is toxic and is not recommended for sheep feeding.

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11.

Influence of progesterone on GAD65 and GAD67 mRNA expression in the dorsolateral striatum and prefrontal cortex of female rats repeatedly treated with cocaine
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Souza, M.F.

; Toniazo, V.M.

; Frazzon, A.P.G.

; Barros, H.M.T.

Brazilian Journal of Medical and Biological Research

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nov 2009, Volume 42 N. 11 Pages 1068 - 1075

Abstract: En | Text: En | PDF: En

Female rats are intensely affected by cocaine, with estrogen probably playing an important role in this effect. Progesterone modulates the GABA system and attenuates the effects of cocaine; however, there is no information about its relevance in changing GABA synthesis pathways after cocaine administration to female rats. Our objective was to investigate the influence of progesterone on the effects of repeated cocaine administration on the isoenzymes of glutamic acid decarboxylase (GAD65 and GAD67) mRNA in brain areas involved in the addiction circuitry. Ovariectomized, intact and progesterone replacement-treated female rats received saline or cocaine (30 mg/kg, ip) acutely or repeatedly. GAD isoenzyme mRNA levels were determined in the dorsolateral striatum (dSTR) and prefrontal cortex (PFC) by RT-PCR, showing that repeated, but not acute, cocaine decreased GADs/β-actin mRNA ratio in the dSTR irrespective of the hormonal condition (GAD65: P < 0.001; and GAD67: P = 0.004). In the PFC, repeated cocaine decreased GAD65 and increased GAD67 mRNA ratio (P < 0.05). Progesterone replacement decreased both GAD isoenzymes mRNA ratio after acute cocaine in the PFC (P < 0.001) and repeated cocaine treatment reversed this decrease (P < 0.001). These results suggest that cocaine does not immediately affect GAD mRNA expression, while repeated cocaine decreases both GAD65 and GAD67 mRNA in the dSTR of female rats, independently of their hormonal conditions. In the PFC, repeated cocaine increases the expression of GAD isoenzymes, which were decreased due to progesterone replacement.

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12.

Quantitative evaluation of Listeria monocytogenes in fresh and processed surubim fish (Pseudoplatystoma sp)
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Souza, V.M. de

; Alves, V.F.

; Destro, M.T.

; De Martinis, E.C.P.

Brazilian Journal of Microbiology

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sep 2008, Volume 39 N. 3 Pages 527 - 528

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

L. monocytogenes é um patógeno psicrotrófico transmitido por alimentos, de importância especial para alimentos minimamente processados. Neste trabalho, a bactéria foi enumerada em amostras de peixe surubim utilizando-se a técnica do NMP. A população de L. monocytogenes foi estimada como < 0.012 NMP/cm² do peixe fresco e < 0.03 NMP/g do peixe minimamente processado.

L. monocytogenes is a foodborne psychrotrophic bacterial pathogen of special importance for minimally processed foods. In this work, it was enumerated in samples of surubim fish by MPN technique. The population of L. monocytogenes was estimated as < 0.012 MPN/cm² in fresh and < 0.03 MPN/g in minimally processed fish.

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13.

Efeitos da umidade relativa do ar na incubadora e da idade da matriz leve sobre o rendimento da incubação
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Barbosa, V.M.

; Cançado, S.V.

; Baião, N.C.

; Lana, A.M.Q.

; Lara, L.J.C.

; Souza, M.R.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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jun 2008, Volume 60 N. 3 Pages 741 - 748

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Avaliaram-se os efeitos da umidade relativa do ar na incubadora (URI) e da idade da matriz leve sobre o rendimento de incubação, sobre o peso absoluto do coração e do saco vitelino e sobre a relação desses dois órgãos com o peso do pinto ao nascer. Foram utilizados 10.836 ovos de matrizes da linhagem Lohmann LSL, com 26, 41 e 56 semanas de idade, incubados com níveis de URI de 48, 56 e 64%. A perda de peso do ovo foi maior à medida que diminuiu a URI ou à medida que aumentou a idade da matriz. A maior taxa de eclosão foi obtida quando se utilizaram 56% de URI, e os ovos das matrizes mais velhas apresentaram eclodibilidade reduzida. A maior relação saco vitelino/peso do pinto foi obtida quando os ovos foram incubados com URI de 48% e com ovos de matrizes com idade de 41 semanas. Os pintos de matrizes com 41 e 56 semanas de idade apresentaram os maiores pesos absolutos do coração e os das matrizes com 56 semanas, a maior relação peso coração/peso pinto.

The effects of hatchery relative air humidity (RAH) and age of breeder hen on incubation yield, on heart weight, on yolk sac and on the ratio of these organs and the newly-hatched chick weight were evaluated. A total of 10,836 eggs of Lohmann LSL of 26, 41 and 56 week-old breeder hens were incubated in three hatchery relative humidity (48, 56 and 64%). Egg weight loss was higher for eggs incubated at lower RAH and for older breeder hens. The hatchability was higher for RAH lower than 56% and lower for eggs of older hens. Higher yolk sac/chick weight ratio was observed for eggs of 41 week-old breeder hens and for eggs incubated at lower RAH (48%).Chicks hatched from eggs of 41 to 56 week-old breeder hens had the highest heart weight/chick weight ratio.

5764 downloads Cited 4 times in SciELO

14.

Estudos sobre a nutrição mineral do milho: II - deficiências de macronutrientes na variedade Piranão
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Malavolta, E.

; Rapchan, V.M.

; Castro, A.M.G. de

; Souza, A.P.

; Lopes, E.S.

; Sobral, L.F.

; Lovato, L.A.

; Menezes, M.

; Tisselli Fº, O.

; Oliveira, G.D.

Anais da Escola Superior de Agricultura Luiz de Queiroz

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1975, Volume 32 Pages 509 - 525

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Foram induzidos sintomas de carência de macronutrientes no milho, var. Piranão, exceto no caso do enxofre. Os níveis foliares de N, P, K, Ca, Mg e S foram determinados nas plantas sujeitos à nutrição completa e às deficiências de macronutrientes.

Corn plants, Piranão variety, were grown in nutrient solution, complete and with omission of one macronutrient each time, in order to gain information on the effects of treatments on growth, symptoms of deficiency, and mineral composition of the leaves. The following symptoms of deficiency were obtained: Nitrogen -- plants underdeveloped; "V" shaped chlorosis beginning in the older leaves followed by drying and necrosis; Phosphorus - older leaves dark green with a purple hve; Potassium - plants underdeveloped; marginal chlorisis and necrosis in the older leaves; iron induced chlorosis in the younger leaves; Calcium - plants very underdeveloped due to death of apical merystem; younger leaves showing tip and marginal chlorosis and afterwards necrosis and break down of the tissue; wide whitish areas in the younger leaves; Magnesium - plants very underdeveloped; internerval chlorosis in older leaves first, proceeding to the young ones; Sulfur - absence of marked symptoms, except for a slight chlorosis in the younger leaves. Dry matter production obeyed the following decreasing order: Complete, minus S, minus P, minus K, minus Mg, minus N and minus Ca. Ears were obtained only in treatments complete and minus S. Top/root ratio decreased according to this order: minus S, complete, minus K, minus Mg, minus N, minus P and minus Ca. The macronutrient content for the treatment complete was the following respectively for lower and upper, leaves: N - 2.76 and 2.52%; P - 0.59 and 0.53%; K - 2.25 and 2.26%;Ca - 2.17 and 1.85;Mg - 0.82 and0.66;S - 0.38 and 0.32. The values of the element corresponding to each treatment were: N - 1.84 and 1.22 (that is: lower leaves in the minus N plants had 1 84% N, whereas the upper leaves in same treatment had 1.22% N; P - 0.14 and 0.17%; K - 0.29 and 0.78%; Ca - 0.29 and 0.05; Mg -0.09 and 0.05%; S- 0.31 and 0.26%.

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