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au:TAVARES, VALERIA R.

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ANTIPROMASTIGOTE AND CYTOTOXIC ACTIVITIES OF FLAVONOIDS FROM Fridericia speciosa LEAVES
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Milani, Reinaldo R.

; Alves, Osvaine J. A.

; Bertanha, Camila S.

; Gimenez, Valéria M. M.

; Squarisi, Iara S.

; Nicolella, Heloiza D.

; Pagotti, Mariana C.

; Tavares, Denise C.

; Silva, Márcio L. A. e

; Cunha, Wilson R.

; Magalhães, Lizandra G.

; Januario, Ana H.

; Pauletti, Patricia M.

Química Nova

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dec 2020, Volume 43 N. 10 Pages 1404 - 1407

Abstract: En | Text: En | PDF: En

Fridericia speciosa (Bignoniaceae) is a liana that occurs in humid to dry vegetation in eastern and southeastern Brazil. Species belonging to the genus Fridericia are employed as medicinal plants. This study evaluates the antipromastigote and cytotoxic activities of the crude extract, fractions, and compounds isolated from F. speciosa, and an HPLC (High-Performance Liquid Chromatography) analytical method was used to quantify the bioactive flavonoids. The flavonoids alpinetin (1), luteolin (2), diosmetin (3), and apigenin (4) were isolated from the active EtOAc-soluble fraction (EAF) of the crude ethanol extract (LCE) obtained from F. speciosa leaves. Both LCE and EAF were active against the normal human lung fibroblast cell line GM07492A (IC50 of 460.7 µg mL-1 and 381.4 µg mL-1, respectively). Only EAF showed activity against Leishmania amazonensis (80.4% at 50 µg mL-1). Compounds 1 and 2 were quantified by HPLC-DAD in both LCE (1: 6.21%; 2: 1.11%) and EAF (1: 45.32%; 2: 2.00%). At 100 µM, compounds1-4 inhibited L. amazonensis flagellar motility by 40.2%, 26.8%, 1.0%, and 24.8%, respectively, while their toxicities (IC50) to the normal cell were > 1000, 353.0, 766.2, and >1000 µg mL-1, respectively. Therefore, F. speciosa leaves are a prolific source of bioactive flavonoids.

https://doi.org/10.21577/0100-4042.20170628 159 downloads

Isolation, in vitro and in silico Evaluation of Phenylethanoid Glycoside from Arrabidaea brachypoda as Lipoxygenase Inhibitor
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Bertanha, Camila S.

; Gimenez, Valéria M. M.

; Furtado, Ricardo A.

; Tavares, Denise C.

; Cunha, Wilson R.

; Silva, Márcio L. A. e

; Januario, Ana H.

; Borges, Alexandre

; Kawano, Daniel F.

; Parreira, Renato L. T.

; Pauletti, Patrícia M.

Journal of the Brazilian Chemical Society

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apr 2020, Volume 31 N. 4 Pages 849 - 855

Abstract: En | Text: En | PDF: En

Lipoxygenase (LOX) plays an important role in inflammatory processes and Arrabidaea brachypoda (DC.) Bureau (Bignoniaceae) has been described as presenting anti-inflammatory activity. Therefore, the objective of this study was to develop a high-performance liquid chromatography (HPLC) procedure to directly recognize LOX inhibitor compounds in A. brachypoda crude extract, facilitating the isolation, characterization of bioactive compounds, evaluation of natural compounds using an in vitro 15-LOX assay and prediction of the most probable binding modes of their main constituent through molecular docking simulations. The chemical analysis was performed by ethanol crude extract microfractionation using HPLC-DAD (diode array detector) associated to a fraction collector. The bioactive chromatogram displayed a peak with 50.9% LOX inhibition at 13.6 min retention time. The extract was purified and conandroside was isolated, presenting a LOX inhibitory activity with an inhibitory concentration (IC50) of 7.8 ± 1.1 µM, close to standard quercetin (IC50 7.6 ± 0.3 µM). Additionally, conandroside was not cytotoxic to normal cells (GM07492A). The LOX-conandroside complex displayed a slightly higher docking score (92.7) than quercetin (71.5). These results together suggest that conandroside could be explored as lipoxygenase inhibitor.

https://doi.org/10.21577/0103-5053.20190248 328 downloads

Efeito da aplicação de ácido giberélico no crescimento da palmeira-ráfia
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Tavares, Armando Reis

; Aguiar, Francismar Francisco Alves

; Sado, Monaly

; Kanashiro, Shoey

; Chu, Edison Paulo

; Lima, Giuseppina Pace Pereira

; Luz, Petterson Baptista da

; Modolo, Valéria Aparecida

Revista Árvore

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dec 2007, Volume 31 N. 6 Pages 999 - 1004

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A palmeira-ráfia possui grande importância econômica devido ao seu interesse comercial. É uma planta ornamental muito apreciada por sua beleza e utilização em ambiente interno, crescendo bem em locais com sombra e apresentando resistência à exposição solar. O valor econômico dessa planta está relacionado à altura e número de hastes. O objetivo do trabalho foi promover o crescimento da planta, através da aplicação do regulador de crescimento ácido giberélico (GA3). Mudas de palmeira-ráfia com aproximadamente um ano de idade foram aspergidas a cada 21 dias (quatro aplicações) com solução de ácido giberélico nas concentrações de 0; 75; 150; 225; e 300 mg.L-1. Os resultados indicaram que a aplicação do ácido giberélico foi eficiente na promoção do crescimento da espécie, sendo esse incremento significativo nas variáveis: comprimento dos pecíolos, lâminas foliares e altura da planta. Não se observou diferença no acúmulo de matéria seca ou fresca do pecíolo entre os tratamentos, e plantas do tratamento-controle foram superiores às dos tratamentos 225 e 300 mg L-1, nas variáveis diâmetro do colo e massa fresca e seca das raízes. Devido ao incremento na altura das mudas e à alteração da disposição das folhas da palmeira, a utilização do ácido giberélico pode ser recomendada para a produção de mudas de alta qualidade.

Lady Palm is of great economic importance due its commercial interest. It is an ornamental plant growing in shadowy places, resistant to sun exposure and very appreciated for its beauty and ornamental value. Its commercial value is associated to plant height and shoot number. The aim of this work was to stimulate lady palm growth by applying the plant growth regulator gibberellic acid. Plantlets of R. excelsa approximately one year old were sprayed every 21 days (4 applications) with gibberellic acid solution at concentrations of 0; 75; 150; 225 and 300 mg L-1. The results showed that gibberellic acid was efficient in promoting the growth of the species and was statistically significant for petioles, leaf length and plant height. Petiole dry and fresh mass did not show any significant difference between treatments for stem diameter, with root dry and fresh mass having a higher value for the control than for the 225 and 300 mg L-1 treatments. Due to the higher height and leaf architecture changes observed, gibberellic acid can be used as a tool to stimulate Lady Palm growth, adding commercial value to the plantlets.

3629 downloads

Screening of Central and South American plant extracts for antimycobacterial activity by the Alamar Blue test
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Leitão, Suzana G.

; Castro, Oscar

; Fonseca, Eduardo N.

; Julião, Lisieux S.

; Tavares, Eliana S.

; Leo, Rodrigo R.T.

; Vieira, Ricardo C.

; Oliveira, Danilo R.

; Leitão, Gilda G.

; Martino, Virginia

; Sulsen, Valeria

; Barbosa, Ymira A.G.

; Pinheiro, Diva P.G.

; Silva, Pedro E. Almeida da

; Teixeira, Dulcineia F.

; N. Junior, Ivan

; Lourenço, Maria Cristina S.

Revista Brasileira de Farmacognosia

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mar 2006, Volume 16 N. 1 Pages 6 - 11

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Quarenta e oito extratos brutos etanólicos e frações (em hexano, diclorometano, acetato de etila e n-butanol) de dez plantas brasileiras pertencentes às famílias Leguminosae, Monimiaceae e Verbenaceae; uma da Costa Rica (Verbenaceae) e uma da Argentina (Verbenaceae) foram ensaiados para verificação da atividade anti-micobacteriana contra Mycobacterium tuberculosis (ATCC-27294H37Rv), pelo teste do Alamar Blue, a uma concentração fixa de 100 µg/mL. Dentre os quarenta e oito extratos e frações estudados, sete mostraram-se ativos na concentração ensaiada - frações em hexano e diclorometano de folhas de Lantana trifolia, extrato em metanol:água, 1:1 de cascas de Vitex cooperi, frações em hexano e diclorometano de folhas de Lippia lacunosa e de Lippia rotundifolia, sendoque todas essas plantas pertencem à família Verbenaceae.

Forty eight ethanolic crude extracts and fractions (hexane, dichloromethane, ethyl acetate and n-butanol) from ten Brazilian plants (Leguminosae, Monimiaceae and Verbenaceae), 1 from Costa Rica (Verbenaceae) and 1 from Argentina (Verbenaceae) were screened for anti-mycobacterium activity against Mycobacterium tuberculosis (ATCC-27294H37Rv), by the Alamar Blue test, at a fixed concentration of 100 µg/mL. Out of the forty eight, seven were active at this concentration, corresponding to Lantana trifolia (hexane and dichloromethane extracts from leaves), Vitex cooperi (methanol:water, 1:1 extract from barks), Lippia lacunosa (hexane and dichloromethane extracts from leaves) and Lippia rotundifolia (hexane and dichloromethane extracts from leaves), all from the Verbenaceae family.

3279 downloads

New records of Uroderma magnirostrum Davis (Mammalia, Chiroptera) from southeastern Brazil, with comments on its natural history
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Nogueira, Marcelo R.

; Tavares, Valéria C.

; Peracchi, Adriano L.

Revista Brasileira de Zoologia

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dec 2003, Volume 20 N. 4 Pages 691 - 697

Abstract: En | Text: En | PDF: En

Uroderma magnirostrum Davis, 1968 is reported from four new localities in southeastern Brazil, significantly extending its geographical distribution south and westward. A total of 12 adult specimens collected in areas of Caatinga and Atlantic Forest in the states of Minas Gerais, Espírito Santo, and Rio de Janeiro, including specimens from the new localities and museum specimens were examined. Females from southeastern Brazil were larger than males in all external measurements analyzed, but after univariate statistical analyses using corrected p values for multiple tests no significant sexual dimorphism was detected. Mean values obtained from this sample fall within the known range documented for the species in both external and cranial measurements, and are similar to those found in specimens from north and northeastern Brazil. The habitats of the new localities of U. magnirostrum in southeastern Brazil ranged from pristine and secondary forests to a small urban park. Uroderma magnirostrum is apparently a rare species in southeast Brazil, which corroborates most previous reports of populations of this bat at other localities.

2418 downloads

Isolation of dengue virus type 1 from larvae of Aedes albopictus in Campos Altos city, State of Minas Gerais, Brazil
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Serufo, José C.

; Oca, Hector Montes de

; Tavares, Valéria A.

; Souza, Andréa M.

; Rosa, Reinaldo V.

; Jamal, Marcos C.

; Lemos, José R.

; Oliveira, Maria A.

; Nogueira, Rita M. R.

; Schatzmayr, Hermann G.

Memórias do Instituto Oswaldo Cruz

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sep 1993, Volume 88 N. 3 Pages 503 - 504

Abstract: En | Text: En | PDF: En

2257 downloads

Investigação sorológica da influenza tipos A e B em estudantes universitários, Brasil
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Mancini, Dalva A. P.

; Tavares, Valéria R.

; Espindola, Clarice P. M.

Revista de Saúde Pública

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dec 1991, Volume 25 N. 6 Pages 468 - 472

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Levantamento sorológico realizado em 200 estudantes da Universidade de São Paulo, nos anos de 1984 e 1985, demonstrou ampla prevalência sorológica do vírus da influenza tipos A e B. Os anticorpos dos indivíduos foram detectados pela técnica de Hemólise Radial Simples (HRS), cujas médias aritméticas de títulos foram maiores entre as cepas dos subtipos (H1N1) e (H3N2) do vírus da influenza tipo A, mais recentemente isoladas da população. Porém, com relação ao tipo B, deste vírus, a situação foi inversa, pois apesar da cepa B/Engl./ 847/73 ser a mais antiga incidente, revelou melhor reatogenicidade sobre as demais cepas avaliadas e de acordo com a doutrina do "Pecado original antigênico", é suposto que tenha sido responsável pela primo infecção na maioria do grupo investigado. A avaliação sorológica dos subtipos do vírus influenza tipos A e B, desta população, revelou índices de anticorpos de baixos títulos HRS (2,5 a 3,5 mm) e de altos títulos (> 4,0 mm) que estão relacionadas ao menor e maior nível de proteção à infecção. Sendo que a capacidade individual da imunidade e da persistência de anticorpos contra o vírus, dependeram da atualidade e freqüência de exposição à influenza.

Wide serological prevalence of influenza A and B was verified by the serological survey covering 200 students of the University of S. Paulo during the 1984-1985 period. The humoral antibodies were detected by the single radial haemolysis technique, whose arithmetic titres averages were greater for both subtypes, (H1N1) and (H3N2) of the influenza A virus strains recently isolated from the population. However, the situation of this type B virus was not the same as that of type A seeing that the B/Engl/ 847/73, although an older strain, showed better reactogenicity than the other strains evaluated. It is possible that is was responsible for the primo infection of most of the components of the group surveyed, as the phenomenon of the "Original Antigenic Sin" explains. The antibody responses to subtypes of influenza A and B in this survey demonstrated levels with low SRH titres (2.5 to 3.5 mm) and high SRH titres (> 4.0mm) related to the lowest and highest levels of suggest protection against infection. Individual immunity and persistence of antibody are related to frequency and recent occurrence of exposition to influenza. As concerns the formulation of influenza vaccine it was established that this preserves the antigen selection of the new strains isolated from the population, mainly due to the behavior of the influenza A virus.

1154 downloads

Investigação sorológica da influenza tipos A e B em estudantes universitários, Brasil
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Mancini, Dalva A. P.

; Tavares, Valéria R.

; Espindola, Clarice P. M.

Revista de Saúde Pública

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dec 1991, Volume 25 N. 6 Pages 468 - 472

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

3989 downloads

Freqüência de anticorpos contra os vesiculovírus e aftovírus, em bovinos e eqüídeos, de Catolândia-Bahia
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Tavares-Neto, José

; Sôndahl, Magnus S.

; Oliveira, Guilherme Ferreira de

; Farah, Samir

; Cortes, Paulo Severo B.

; Molina, Carlos Augusto F.

; Cassis Neto, Domingos

; Freitas, Frederico A. S.

; Silva, Guilherme R.

; Gonçalves, Jussara

; Gonçalves, Raquel M.

; Alves, Valéria Cardoso

; Brito, Vinícius Nahime de

; Facure Júnior, Waldir

Revista da Sociedade Brasileira de Medicina Tropical

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sep 1991, Volume 24 N. 3 Pages 177 - 179

Abstract: Pt | Text: Pt | PDF: Pt

1431 downloads

10.

A ocorrência de riquetsioses do grupo Rickettsia rickettsii
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Mancini, Dalva A. Portari

; Nascimento, Elvira M. Mendes

; Tavares, Valéria Rosa

; Soares, Murillo Adelino

Revista de Saúde Pública

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dec 1983, Volume 17 N. 6 Pages 493 - 499

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Foi realizada revisão da literatura com objetivo de atualizar as informações sobre a ocorrência de riquetsioses do grupo Rickettsia rickettsii. Verificou-se que nos EUA e Europa, a incidência da febre maculosa, vem aumentando desde 1970 até hoje. No Brasil, foi relatado um caso presuntivo, no estado da Bahia, em 1979. Com relação a prevenção, controle e tratamento dessa doença é salientada a importância de informações relacionadas com indivíduos expostos a picadas de carrapatos, notificação de novos casos, fatores ecológicos, técnicas laboratoriais mais específicas para a identificação do agente etiológico, e a antibioticoterapia mais eficiente. A vacinação é ainda referida como meio mais favorável na prevenção da doença, devendo ser administrada aos indivíduos de alto risco. No Brasil, faltam informações precisas sobre a ocorrência de R. rickettsii.

A search of the literature to update the available information on the occurrence of rickettsiosis caused by the Rickettsia rickettsii group was made. It was verified that the incidence of spotted fever has had an increase in the U.S.A. and Europe since 1970. In Brazil, a presumptive case was reported in the State of Bahia, in 1979. Regarding the prevention, control and treatment of this disease, importance is given to data related to individuals exposed to tick bites, report of new cases, ecological factors, more specific laboratorial procedures for the identification of the etiological agent, and a more efficient antibiotic therapy. Vaccination is still regarded as the most adequate means for the prevention of the disease, and should be aimed at groups of individuals at high risk. In Brazil, there is a lack of more precise information on the occurrence of R. rickettsii.

1960 downloads

11.

A ocorrência de riquetsioses do grupo Rickettsia rickettsii
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Mancini, Dalva A. Portari

; Nascimento, Elvira M. Mendes

; Tavares, Valéria Rosa

; Soares, Murillo Adelino

Revista de Saúde Pública

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dec 1983, Volume 17 N. 6 Pages 493 - 499

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

5868 downloads

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