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Influencia de bioestimulantes sobre caracteres morfológicos y agroquímicos del banano (Musa AAA cv. Williams)
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Ugarte-Barco, Francisco Andrés

; Zhiñin-Huachun, Iván Armando

; Hernández-Pérez, Ricardo

Terra Latinoamericana

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dec 2022, Volume 40 elocation e1456

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

RESUMEN: Una de las limitantes para la aclimatación de plantas in vitro en el cultivo del banano ha sido el pobre enraizamiento y su lento desarrollo foliar, que afecta la sobrevivencia en campo bajo condiciones de stress posterior a siembra. El objetivo fue comparar la respuesta de plántulas de Musa cv. Williams tratadas con bioestimulantes y desestresantes comerciales. A los 30 días después de la inoculación, se determinó el diámetro del pseudotallo (cm), masa fresca de planta (g), masa fresca radical (g), número de raíces, índice SPAD, contenido iónico edáfico y foliar (pH, CE, NO3 -, K+, Na+ y Ca2+). Los resultados preliminares indicaron que StresSal® y (StresSal® + Equilibrium®) mostraron de forma significativa un mayor diámetro de pseudotallo. La mayor masa fresca radical correspondió a (Inicium® + StresSal®). El número de raíces no fue significativamente diferente. El índice SPAD fue elevado cuando se aplicó StresSal® solo o con Equilibrium®. El tratamiento (Inicium®+StresSal®) contribuyó a la mayor acidez en suelo. La conductividad (CE), el K+ y Na+ fueron mayores aplicando (Inicium® + Pro-Fulvic®). Mientras los niveles de NO3 - aumentaron con Inicium®. Los iones Ca2+ disminuyeron significativamente en suelo con (Inicium® + StresSal®). En cuanto al contenido foliar, el pH más alto correspondió a Inicium®, la conductividad (CE) fue mayor con StresSal® e (Inicium® + StresSal®), sin diferencia con el testigo. Un aumento de NO3 - se halló con (Inicium® + StresSal®). Mientras Na+ y Ca2+ fueron significativamente mayores con (Inicium® + Pro-Fulvic®).

SUMMARY: Some of the limitations for plant acclimatization in vitro in banana cultivation have been poor rooting and slow leaf development, which affect survival in the field under stress conditions after planting. The aim of this study is to compare seedling response of Musa cv. Williams treated with biostimulants and commercial de-stressing agents. Thirty days after inoculation, pseudostem diameter (cm), fresh plant mass (g), fresh root mass (g), root number, soil-plant analysis development (SPAD) index, edaphic ionic content and foliar content (pH, CE, NO3 -, K+, Na+ and Ca2+) were evaluated. Preliminary results indicated, that StresSal® and (StresSal® + Equilibrium®) showed significantly greater pseudostem diameter. The highest radical fresh mass corresponded to (Inicium® + StresSal®). Root number was not significantly different. The SPAD index was elevated when StresSal® was applied alone or with Equilibrium®. The treatment (Inicium®+StresSal®) contributed to higher soil acidity. Conductivity (CE), K+ and Na+ were higher when Inicium® + Pro- Fulvic® were applied. As NO 3 - levels increased with Inicium®, Ca+2 ions decreased significantly in soil with Inicium® + StresSal®. Regarding foliar content, the highest pH corresponded to Inicium®, conductivity (CE) was higher with StresSal® and Inicium® + StresSal®, without difference with the control. An increase in NO3 - was found with Inicium® + StresSal®. While Na+ and Ca2+ were significantly higher with Inicium® + Pro-Fulvic®.

https://doi.org/10.28940/terra.v40i0.1456 83 downloads

Infección respiratoria aguda por coronavirus Sars-CoV-2 en personal de salud. Implementación de un programa de detección precoz y seguimiento de casos en un hospital universitario
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Poblete Umanzor, Rodrigo

; Saldías Peñafiel, Fernando

; Sabatini Ugarte, Natalia

; Vite Valverde, Andy

; Ceriani Bravo, Alejandro

; Schaffeld Pernas, Soledad

; Letelier Saavedra, Luz María

; Gran Scheuch, José Ignacio

; Rabagliati Borie, Ricardo

Revista médica de Chile

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jun 2020, Volume 148 N. 6 Pages 724 - 733

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Background: Healthcare personnel are a high-risk group for acquiring COVID-19 disease. They represent 4 to 20% of the total number of cases reported in different geographical areas. Aim: To describe an epidemiological surveillance strategy to detect symptoms compatible with SARS-CoV-2 infection and early case detection among healthcare personnel at a university hospital. Patients and Methods: We assessed 209 healthcare workers reporting symptoms suggestive of COVID-19 in a telephone counseling system. After a structured evaluation, the suggestions ranged from having a SARS-CoV-2 nasopharyngeal swab PCR test, consulting in the emergency room or at outpatient clinic or returning to work. Results: In 61% of assessed workers a coronavirus SARS-CoV-2 nasopharyngeal swab PCR was requested and 28 (22%) were positive. In a multivariate analysis, the clinical variables associated with a positive PCR test were the presence of fever, sudden loss of smell or taste, and a history of contact with a COVID-19 positive case. Conclusions: The telephone symptom monitoring program allowed the early detection of a significant number of healthcare officials with acute respiratory infection due to coronavirus SARS-CoV-2, it is easy to implement and has a low cost.

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Efecto de los antimaláricos sobre los diferentes dominios del índice de daño SLICC en pacientes de la cohorte GLADEL
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Pons-Estel, Guillermo J.

; Quintana, Rosana

; Wojdyla, Daniel

; Alarcón, Graciela S.

; Serrano, Rosa María

; Ugarte-Gil, Manuel

; Pimentel-Quiroz, Víctor

; Soriano, Enrique R.

; Catoggio, Luis J.

; Scolnik, Marina

; Sacnun, Mónica

; Saurit, Verónica

; Caeiro, Francisco

; Alvarellos, Alejandro

; Sarano, Judith

; García, Mercedes

; Onetti, Laura

; Drenkard, Cristina

; Berbotto, Guillermo

; Scherbarth, Hugo R.

; Sato, Emilia

; Bonfa, Eloisa

; Ferreira Borba, Eduardo

; Costallat, Lilian

; Xavier, Ricardo

; Tavares Brenol, Joao C.

; Da Silva, Nilzio A.

; Cavalcanti, Fernando

; Massardo, Loreto

; Jacobelli, Sergio

; Neira, Oscar

; Molina, José F

; Vásquez, Gloria

; Gómez-Puerta, José A.

; Alonso Gonzalez, Luis

; Iglesias Gamarra, Antonio

; Guibert-Toledano, Marlene

; Reyes, Gil A.

; Cardiel, Mario H.

; Pascual-Ramos, Virginia

; García de la Torre, Ignacio

; Barile, Leonor

; Silveira, Luis H.

; Amigo, Mary-Carmen

; Sauza del Pozo, María Josefina

; Acevedo-Vásquez, Eduardo M.

; Alfaro-Lozano, José

; Segami, María Inés

; Chacón-Díaz, Rosa

; Abadi, Isaac

; Esteva Spinetti, María H

; Pons-Estel, Bernardo A.

Revista argentina de reumatología

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sep 2018, Volume 29 N. 3 Pages 6 - 10

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Objective: To assess the effects of antimalarials (AM) over the items of the SLICC Damage Index (SDI). Methods: Patients with recent (≤2 years) diagnosis of systemic lupus erythematosus (SLE) from the GLADEL cohort were studied. End-point: increase in items SDI since cohort entry. Independent variables (socio-demographic, clinical, laboratory and treatment) were included. The effect of AM as a time dependent variable on most frequent SDI items (adjusting for potential confounders) was examined with a multivariable Cox regression model. Results: Of the 1466 patients included in this analysis, 1049 (72%) received AM with a median exposure time of 30 months (Q1-Q3: 11-57). Damage occurred in 665 (45%) patients during a median follow-up time of 24 months (Q1-Q3: 8-55). There were 301 integument, 208 renal, 149 neuropsychiatric, 98 musculoskeletal, 88 cardiovascular and 230 others less frequently represented damages. After adjusting for potential confounders at any time during follow-up, a lower risk of renal damage (HR 0.652; 95% CI: 0.472-0.901) and borderline for neuropsychiatric damage (HR 0.701, 95% CI: 0.481-1.024) was found. Conclusion: In the GLADEL cohort, after adjustment for possible confounding factors, AM were independently associated with a reduced risk of renal damage accrual.

Objetivos: Estimar el efecto de los antimaláricos (AM) sobre los diferentes dominios del índice de daño SLICC (SDI). Métodos: Se estudiaron pacientes con diagnóstico clínico reciente (≤2 años) de lupus eritematoso sistémico (LES) de la cohorte GLADEL. Variable de estudio: aumento en los dominios del SDI desde el ingreso a la cohorte. Variables independientes: características sociodemográficas, clínicas, laboratorio y tratamientos. El efecto de los AM, como variable dependiente del tiempo, sobre los dominios más frecuentes del SDI (ajustado por factores de confusión) fue examinado con un modelo de regresión de Cox multivariado. Resultados: De 1466 pacientes estudiados, 1049 (72%) recibieron AM con un tiempo medio de exposición de 30 meses (Q1-Q3: 11-57) y 665 pacientes (45%) presentaron daño durante un seguimiento medio de 24 meses (Q1-Q3: 8-55); 301 eventos fueron cutáneos, 208 renales, 149 neuropsiquiátricos, 98 musculoesqueléticos, 88 cardiovasculares y 230 otros. Después de ajustar por factores de confusión, el uso de AM se asoció a un menor riesgo de daño renal (HR 0,652; IC 95%: 0,472-0,901) y en el límite de la significancia estadística (HR 0,701, IC 95%: 0,481-1,024) para el dominio neuropsiquiátrico. Conclusión: En GLADEL, el uso de AM se asoció independientemente a un menor riesgo de daño acumulado renal.

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Predictores de la percepción materna del peso del hijo con sobrepeso-obesidad
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Flores-Peña, Yolanda

; Trejo-Ortiz, Perla María

; Ávila-Alpirez, Hermelinda

; Cárdenas-Villarreal, Velia Margarita

; Ugarte-Esquivel, Alicia

; Gallegos-Martínez, Josefina

; Cerda-Flores, Ricardo Martín

Universitas Psychologica

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jun 2014, Volume 13 N. 2 Pages 553 - 563

Abstract: Es En | Text: Es En | PDF: Es | PDF: En

Se identificaron predictores de la percepción materna del peso del hijo (PMPH) con sobrepeso - obesidad (SP- OB). Participaron 2.874 madres e hijos, residentes en el Noreste de México; 951 tenían un hijo con SP-OB. La PMPH se evaluó por palabras (PP) y por imágenes (PI). Por PP 63.71% y 74.9% de madres de hijos con SP-OB no percibieron adecuadamente el peso del hijo y por PI, 27% y 10.99%. Mediante regresión logística, el modelo para PP fue χ² = 152.05, gl = 9, p = 0.001, varianza explicada 21.5% y para PI fue χ² = 192.35, gl = 9, p = 0.001, varianza explicada 31.9%. Edad del hijo y si el médico informa de SP en el niño/a, fueron variables que contribuyeron a los modelos. Se concluye que las madres de niños con SP-OB no perciben de forma adecuada esta situación. Se resalta la importancia de informar respecto al SP-OB del hijo.

Several predictors of a maternal perception of her child's weight (MPCW) when the child is overweigh-obese (OW-OB) were identified. Participants: 2,842 mothers and children who reside in Mexico's northeastern region, 951 mothers had an OW-OB child. The MPCW was evaluated for words (PW) and images (PI). PW, 63.71% and 74.9% of the mothers with an OW-OB child, did not perceive the child's weight adequately and PI, 27% and 10.99%. Through Logistic Regression Analysis, a model for PW was χ² = 152.05, gl = 9, p = 0.001, 21.5% explained variance; and for PI χ² = 192.35, gl = 9, p = 0.001, 31.9% explained variance. The child's age, a doctor's report that the child had OW, were some variables that contributed to the models. We concluded that mothers with OW-OB children do not adequately perceive this situation. We emphasize the importance of reporting of a child's OW-OB condition.

https://doi.org/10.11144/Javeriana.UPSY13-2.ppmp 2368 downloads

Clinical characteristics of chronic kidney disease of nontraditional causes in Salvadoran farming communities
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Herrera, Raúl

; Orantes, Carlos M.

; Almaguer, Miguel

; Alfonso, Pedro

; Bayarre, Héctor D.

; Leiva, Irma M.

; Smith, Magaly J.

; Cubias, Ricardo A.

; MD, Carlos G. Torres

; Almendárez, Walter O.

; Cubias, Francisco R.

; Morales, Fabrizio E.

; Magaña, Salvador

; Amaya, Juan C.

; Perdomo, Edgard

; Ventura, Mercedes C.

; Villatoro, Juan F.

; Vela, Xavier F.

; Zelaya, Susana M.

; Granados, Delmy V.

; Vela, Eduardo

; Orellana, Patricia

; Hevia, Reynaldo

; Fuentes, E. Jackeline

; Mañalich, Reinaldo

; Bacallao, Raymed

; Ugarte, Mario

; Arias, María I.

; Chávez, Jackelin

; Flores, Nelson E.

; Aparicio, Claudia E.

MEDICC Review

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apr 2014, Volume 16 N. 2 Pages 39 - 48

Abstract: En | Text: En | PDF: En

INTRODUCTION: Chronic kidney disease is a serious health problem in El Salvador. Since the 1990s, there has been an increase in cases unassociated with traditional risk factors. It is the second leading cause of death in men aged >18 years. In 2009, it was the first cause of in-hospital death for men and the fifth for women. The disease has not been thoroughly studied. OBJECTIVE: Characterize clinical manifestations (including extrarenal) and pathophysiology of chronic kidney disease of nontraditional causes in Salvadoran farming communities. METHODS: A descriptive clinical study was carried out in 46 participants (36 men, 10 women), identified through chronic kidney disease population screening of 5018 persons. Inclusion criteria were age 18 - 59 years; chronic kidney disease at stages 2, 3a and 3b, or at 3a and 3b with diabetes or hypertension and without proteinuria; normal fundoscopic exam; no structural abnormalities on renal ultrasound; and HIV-negative. Examinations included social determinants; psychological assessment; clinical exam of organs and systems; hematological and biochemical parameters in blood and urine; urine sediment analysis; markers of renal damage; glomerular and tubular function; and liver, pancreas and lung functions. Renal, prostate and gynecological ultrasound; and Doppler echocardiography and peripheral vascular and renal Doppler ultrasound were performed. RESULTS: Patient distribution by chronic kidney disease stages: 2 (32.6%), 3a (23.9%), 3b (43.5%). Poverty was the leading social determinant observed. Risk factor prevalence: agrochemical exposure (95.7%), agricultural work (78.3%), male sex (78.3%), profuse sweating during work (76.3%), malaria (43.5%), NSAID use (41.3%), hypertension (36.9%), diabetes (4.3%). General symptoms: arthralgia (54.3%), asthenia (52.2%), cramps (45.7%), fainting (30.4). Renal symptoms: nycturia (65.2%), dysuria (39.1%), foamy urine (63%). Markers of renal damage: macroalbuminuria (80.4%), β2 microglobulin (78.2%), NGAL (26.1%). Renal function: hypermagnesuria (100%), hyperphosphaturia (50%), hypernatriuria (45.7%), hyperkaluria (23.9%), hypercalciuria (17.4%), electrolyte polyuria (43.5%), metabolic alkalosis (45.7%), hyponatremia (47.8%), hypocalcemia (39.1%), hypokalemia (30.4%), hypomagnesemia (19.6%). Imaging: Ultrasound showed fatty liver (93.5%) and vascular Doppler showed tibial artery damage (66.7%). Neurological symptoms: abnormal tendon reflexes (45.6%), Babinski sign and myoclonus (6.5%), sensorineural hearing loss (56.5%). CONCLUSIONS: This chronic kidney disease studied behaves clinically like chronic tubulointerstitial nephropathy, but with systemic manifestations not attributable to kidney disease. While male agricultural workers predominated, women and adolescents were also affected. Findings support a hypothesis of multifactorial etiology with a key role played by nephrotoxic environmental agents.

1846 downloads

Growth Hormone Releasing Peptide 6 (GHRP6) reduces liver fibrosis in CCl4 chronically intoxicated rats
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Berlanga-Acosta, Jorge

; Vázquez-Blomquist, Dania

; Cibrián, Danay

; Mendoza, Yssel

; Ochagavía, María E

; Miranda, Jamilet

; Suárez, José

; González-Ferrer, Yolanda

; Vila, José M

; Abreu, Angel

; Ugarte-Moreno, Dayana

; Cruz, Yolanda

; Howland, Ivon

; Coro-Antich, Rosa

; León, Olga S

; Bringas, Ricardo

; García del Barco, Diana

; Cosme-Díaz, Karelia

; Palenzuela, Daniel

; Fernández, Julio R

; Nazabal, Marcelo

; Guillén, Isabel

; Cintado, Alberto

; Inés, Lidia

; López-Mola, Ernesto

; Guillén-Nieto, Gerardo E

Biotecnología Aplicada

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jun 2012, Volume 29 N. 2 Pages 60 - 72

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Tissue fibrosis is a leading cause of morbidity and mortality. Current treatments for conditions such as hepatic fibrosis have been unsuccessful. The growth hormone relasing peptide 6 (GHRP6) is endowed with cardioprotective actions but its antifibrotic effect had not been anticipated. We examined the GHRP6 ability to prevent and revert liver cirrhosis after induction in Wistar rats by a subcutaneous administration of CCl4. GHRP6 effects were examined after concomitant and delayed administration to toxic respectively. The percentages of hepatic fat, fibrosis, nodularity and septae thickness were histologically and morphometrically determined. Ascitis and portal dilation were judged by ultrasound and serum biochemical profile and oxidative stress parameters determined. Mechanistic involvement of selective gene/proteins was assessed by RT-PCR and immunohistochemistry. Microarrays showed gene expression profiles of GHRP6-treated liver samples on CapitalBio Rat Genome Oligo Array. GHRP6 concomitant intervention prevented in more than 85% parenchymal fibrotic induration (p < 0.0001) and therapeutic administration for only 15 days allowed for 37% fibrotic clearance (p = 0.0004) with more than 30% reduction of septae thickness (p = 0.0011). The 60 days GHRP6 administration scheme produced a 75% reduction of the fibrotic area with more than 60% reduction of nodularity. GHRP6 reduced oxidative damage enhancing the activity of antioxidant enzymes. Vimentin and alpha smooth muscle actin immunodetection profile indicated GHRP6 reduced the number of activated stellate cells. GHRP6 administration reduced fibrogenic factors as TGF-ß and CTGF on Kupffer cells. Differentially expressed genes in the microarray experiment indicated GHRP6 modulate the redox balance and parenchymal cells response to injury. These evidences suggest GHRP6 may control the liver's fibroplastic response.

La fibrosis es causa fundamental de morbilidad y mortalidad. Los tratamientos actuales han fracasado. El péptido liberador de la hormona de crecimiento-6 (GHRP6) ejerce efectos cardioprotectores, pero no se ha descrito su acción antifibrótica. Se examinó la propiedad del GHRP6 para prevenir y revertir la cirrosis hepática, luego de su inducción en ratas mediante la administración de CCl4. Se evaluó el porcentaje de grasa hepática, fibrosis, nodularidad y grosor septal mediante estudios histomorfométricos y la ascitis o dilatación portal por ultrasonido. Se determinó el perfil bioquímico y los parámetros de estrés oxidativo en suero, así como la participación de genes y proteínas, mediante reacción en cadena de la polimerasa con transcripción inversa e inmunohistoquímica. Se utilizó un microarreglo de oligonucleótidos del genoma de rata para estudiar el perfil de expresión de los genes inducidos por el GHRP6. La intervención concomitante con GHRP6 previno la induración fibrótica en más del 85% (p < 0.0001). La administración terapéutica durante 15 días permitió la remoción fibrótica del 37% (p = 0.0004), la reducción del grosor septal superó el 30% (p = 0.0011). La administración durante 60 días redujo las áreas fibróticas en 75%, y la reducción de la nodularidad fue de más del 60%. El GHRP6 redujo el daño oxidativo porque aumentó la actividad de las enzimas antioxidantes y las células estrelladas activadas positivas a vimentina y actina alfa de músculo liso. También redujo la expresión de factores fibrogénicos sobre las células Kupffer. El perfil de expresión de genes indicó que el GHRP6 modula el balance redox y la respuesta al daño tisular en las células parenquimales. Estas evidencias sugieren que el GHRP6 pudiera controlar la respuesta fibroplásica del hígado.

215 downloads

Hidrocolpos e hidrometrocolpos neoanatal: a propósito de un caso clínico
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GARFIAS VON F, CAROLINA

; BARRERA N, ANTONIO

; ZUBIETA A, RICARDO

; UGARTE P, FRANCISCA

Revista chilena de pediatría

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apr 2011, Volume 82 N. 2 Pages 137 - 141

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

Hydrocolpos and hydrometrocolpos is a condition characterized by a cystic dilatation od the vagina and/or uterus with accumulation of fluid as a result of congenital vaginal obstructions. It can be of secretory or urinary types, the last one when a vagino-vesical communication develops such as a sinus or chloaca. Other causes are vaginal septum, imperforated hymen. Clinical Case: Ten day old newborn, 46 XX with genital virilization (Prader IV) confirmed as due to Congenital Adrenal Hyperplasia, sonogram showed dilated vagina with fluid content due to neonatal hydrocolpos. Conclusion: It is important to maintain a high index of suspicion when a female newborn shows urogenital sinus, chloaca, genital virilization or imperforated hymen, as well as a female newborn with an abdominal mass. Diagnostic test of choice is a sonogram. Evaluation must be completed by a multidisciplinary team, including urology, endocrinology and pediatric gynecology for optimal patient management.

El Hidrocolpos e Hidrometrocolpos es una condición caracterizada por dilatación quística de la vagina y/o del útero, con acumulación de líquido como resultado de obstrucciones vaginales congénitas. Puede ser de tipo secretorio o urinario, este último ocurre cuando existe comunicación vagino-vesical, como en el seno urogenital o anomalía tipo cloaca. Otras causas son septo vaginal, himen imperforado, malformación tipo cloaca y senourogenital. Caso: Recién nacida de 10 días, 46 XX, con virilización de genitales grado IV de Prader, cuyo estudio confirmó una Hiperplasia Suprarrenal Congénita y cuya ecografía demostró una vagina dilatada con contenido liquido correspondiendo a un hidrocolpos neonatal.

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THEORETICAL STUDY OF THE ISOMERIZATION OF MALEIC ACID INTO FUMARIC ACID
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UGARTE, RICARDO

; BUSTOS, CARLOS

; MORENO-VILLOSLADA, IGNACIO

Journal of the Chilean Chemical Society

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2011, Volume 56 N. 2 Pages 656 - 662

Abstract: En | Text: En | PDF: En

The isomerization reaction of 2-butenedioic acid in gas phase has been studied using Hartree-Fock (HF), Meller-Plesset to 2nd order (MP2) and BLYP, B3LYP Density Functional Theory (DFT) levels of approximation. Potential energy surfaces (PES) of the singlet and triplet states of the molecule are constructed in order to corroborate the hypothesis that the reaction proceeds by a non-adiabatic path. A total of ten stationary points (eight singlet and two triplet) were characterized as energy minima or saddle points. Thermochemical analysis of the triplet conformers and singlet most stable structures yields a value of the AH0 of activation for isomerization that is in good agreement with the expected results.

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Estudio teórico de la isomerización del ácido maleico en ácido fumárico: un enfoque basado en el concepto de superficie de energía potencial
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Ugarte, Ricardo

; Salgado, Guillermo

; Martinez, Rolando

; Navarrete, José

Química Nova

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2010, Volume 33 N. 3 Pages 750 - 754

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

A comparative study based on potential energy surfaces (PES) of 2-butanedioic and hypothetic 2-butanedioic/HCl acids is useful for understanding the maleic acid isomerization. The PES enables locating conformers of minimum energy, intermediates of reactions and transition states. From contour diagrams, a set of possible reaction paths are depicted interconnecting the proposed structures. The study was carried out in absentia and in the presence of the catalyst (HCl), using an solvatation model provided by the Gaussian software package. Clearly, the effect of HCl is given by new reaction paths with lower energetic barriers in relation to the reaction without catalyzing.

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10.

SYNTHESIS AND CONFORMATIONAL ANALYSIS OF LEPTOCARPIN DERIVATIVES: INFLUENCE OF MODIFICATION OF THE OXIRANE RINGON LEPTOCARPIN'S CYTOTOXIC ACTIVITY
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MARTÍNEZ, ROLANDO

; KESTERNICH, VICTOR

; CARRACSO, HÉCTOR

; ÁLVAREZ-CONTRERAS, CAROLINA

; MONTENEGRO, CAROLINA

; UGARTE, RICARDO

; GUTIÉRREZ, ELENA

; MORENO, JOSÉ

; GARCÍA, CARLOS

; WERNER, ENRIQUE

; CÁRCAMOS, JUAN

Journal of the Chilean Chemical Society

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dec 2006, Volume 51 N. 4 Pages 1010 - 1014

Abstract: En | Text: En | PDF: En

The reaction in acidconditions of Leptocarpin 1, a compound with antitumor activity, formed two new isomeric products, 8b-angeloyl-1b,3b-dihydroxy-4,10-dimethyl,-D11(13) methylen-4Z,9Z-dieneheliangol-6,12-olide 2 and 8b-angeloyl-1b,3b-dihydroxy-4-methyl-D11(13),D11(14)-dimethylen-4Z-eneheliangol-6,12-olide 3, whose structures reported in this study were established by spectroscopy (¹H-NMR, 13C-NMR, MS and IR) and confirmedthrough ROESY experiments and theoretical studies by molecular mechanics. The in vitro cytotoxicity of these isomeric compounds was less active than leptocarpin, showing the importance of the oxirane ring in the biological activity. Cytotoxic activity was measured in six cancer cell lines

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11.

Diabetes Mellitus tipo 1 (DM1) en niños menores de 5 años: Características al debut vs otros grupos etarios en Chile
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Eyzaguirre C, Francisca

; Peláez D, José Miguel

; Sepúlveda R, Carolina

; Gaete V, Ximena

; Codner D, Ethel

; Unanue M, Nancy

; Ávila A, Alejandra

; Ugarte P, Francisca

; Silva A, Ricardo

; García B, Hernán

Revista chilena de pediatría

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aug 2006, Volume 77 N. 4 Pages 375 - 381

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

: During the last years, an increase in the incidence of DM1 in infants has been observed. Objective: to study the number of children younger than 5 years-old diagnosed with DM1 and compare clinical and laboratory features with older children at DM1 onset. Method: Study of the clinical and laboratory characteristics in subjects diagnosed with DM1 from 1998 to 2003 in Santiago. Patients were classified according to age in 3 groups: 0-4 (GI), 5-9 (GII) and 10-14 years-old (GIII). Results: 19,7% cases were younger than 5 years-old (GI n = 27), GII (43,8% n = 60) and GIII (36,5% n = 50). A shorter duration of symptoms was observed in GI (18,4 ± 23,7 vs 26,4 ± 27,4) (p < 0,0001) and HbA1c levels were lower in GII (10,1 ± 1,7 vs 11,8 ± 3,4%) (p < 0,0001) and in GIII (12,4 ± 2,6%) (p = 0,028). Glucose levels were similar among groups (p>0,05) and metabolic acidosis was more severe in GI (pH 7,14 ± 0,1 vs 7,19 ± 0,2 in GII and 7,26 ± 0,1 in GIII) (p < 0,0001). A concomitant infectious disease was observed in GI (33%), GII (20%) and GIII (28%) (p > 0,05). Conclusions: An important percentage of DM1 in children presents in subjects younger than 5 years-old. This group showed acute and severe clinical presentation with longer duration of symptoms, severe acidosis and lower HbA1c levels. It is necessary to evaluate carefully in order to suspect the diagnosis in this group

Introducción: En los últimos años, se ha visto un aumento en la incidencia de DM1 en niños. Objetivo: Determinar frecuencia y características clínicas y de laboratorio al debut de DM1 en niños chilenos menores de 5 años, comparado con los de mayor edad. Pacientes y Métodos: Se estudiaron los datos clínicos y de laboratorio de pacientes que debutaron entre 1998-2003 en cuatro centros de Santiago. Se clasificaron en 3 grupos etarios (G): 0-4 (GI), 5-9 (GII) y 10-14 años (GIII) y se compararon según los parámetros descritos. Resultados: Un 19,7% de los pacientes eran menores de 5 años; GI (n = 27), seguido de aquéllos pertenecientes a GII (43,8%; n = 60) y GIII (36,5%; n = 50). El periodo de síntomas previo al diagnóstico fue más corto en GI; 18,4 ± 23,7 vs 26,4 ± 27,4 y 40,1 ± 60 días (p < 0,0001) y su nivel de HbA1c fue más baja; 10,1 ± 1,7 vs 11,8 ± 3,4% en GII (p < 0,0001) y 12,4 ± 2,6% en GIII (p = 0,028), respectivamente. No hubo diferencias en la glicemia inicial entre los grupos. La acidosis metabólica fue mayor en GI; pH 7,14 ± 0,1 vs 7,19 ± 0,2 (GII) y 7,26 ± 0,1 (GIII) (p < 0,0001) y presentaron más infecciones concomitantes (33%, 20 y 28% respectivamente; p > 0,05). Conclusiones: Un porcentaje importante de las DM1 se inicia en niños < 5 años. Este grupo presenta un cuadro más grave, con mayor acidosis, menores niveles de HbA1c y periodo previo de síntomas, por lo que debe existir alerta para el diagnóstico en este grupo etario

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12.

Quimioluminiscencia electrogenerada del luminol usando electrodos de bajo costo
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Salgado, Guillermo

; Navarrete, José

; Bustos, Carlos

; Sánchez, Cristián

; Ugarte, Ricardo

Química Nova

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apr 2006, Volume 29 N. 2 Pages 381 - 384

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

The purpose of the study was to observe eletrogenerated chemiluminescence (ECL) of luminol using different materials as electrodes such as wires, nails, coins, razor blades, etc. Based on the experimental observations and the mechanisms proposed in the literature, students should be capable of analyzing their results in order to understand the phenomena studied. Students could then elaborate a mechanism of action consistent with the experimental results which could rationalize the formation of intermediates in the reaction and the dependence of ECL on solution pH in addition to other points of interest

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