Quality plays an important role in the criteria directing wine product development. The evaluation of sensory characteristics associated with wine quality, as perceived by industry professionals, is therefore important. We investigated the suitability of the free-sorting sensory evaluation method, in combination with wine quality scoring using a 20-point scoring system, to determine the drivers of quality. Eight commercial South African Sauvignon Blanc wines were assessed by a panel of 24 wine industry professionals. Free sorting with a verbalisation step to describe the groups, followed by quality scoring using score sheets routinely used in the wine industry, was performed. A multivariate sensory map was constructed using DISTATIS to explain the similarities and differences amongst the set of wines. Correspondence analysis (CA) was applied to the group descriptors, and CA deviates were calculated. Pearson's correlation coefficients between CA deviates and the quality scores were calculated to identify the drivers of quality. Significant differences in quality were observed between the wines. The sensory attributes "passion fruit", "green pepper", "peas", "asparagus" and "green" were frequently cited by the panel for the wines that received the highest average quality scores, and these attributes were identified as drivers of quality. In this study, a procedure is presented that combines sorting and quality scoring to investigate the relationship between sensory attributes and quality scores to identify the drivers of wine quality. Industry professionals and research environments can use this procedure to determine drivers of wine quality in a single evaluation session.

Large differences in reduced glutathione (GSH) levels have been found in different investigations, also in healthy people. GSH oxidation in vitro has been associated with sample acidification in the presence of oxihemoglobin. In this work, the influence of different acids on GSH determination utilizing HPLC with UV detection was evaluated. The results showed that metaphosphoric acid and sulfosalicylic acid were inadequate for analysis, because metaphosphoric acid showed to be inefficient for deproteinization and with sulfosalicylic acid loss of GSH was observed. Trichloroacetic acid did not effect GSH quantification, since the deproteinized form was immediately derivatized with 5, 5´-dithio-bis (2-nitrobenzoic) acid. Methods with TCA deproteinization presented linear results from 0.5 to 3.0 mM. The correlation coefficient between aqueous curves and GSH spiked RBC exceeded 0.99. Precision calculations showed CV lower than 10% and bias within ± 10% for concentrations of 0.5; 1.5 and 3.0 mM GSH. The recovery was higher than 94%. Moreover, GSH blood concentrations were independent of hemoglobin concentrations.

O tempo de desenvolvimento na câmara incubadora dos embriões do cladócero Penilia avirostris Dana, 1852, foi estimado a partir do zooplâncton coletado na água superficial da Baía de Guanabara, Brasil, diariamente, durante 15 dias. A cada dia, foi anotado o estágio de maturidade dos embriões de 90 fêmeas partenogenéticas. O tempo de desenvolvimento total (do ovo até o nascimento) variou de 2 a 3 dias, sendo a fase imatura (estágios I e IV) geralmente mais longa (2 dias) que as fases intermediárias e maturas (1 dia, estágios V a XII). Resultados similares foram obtidos com a equação de Bottrell, que leva em conta a temperatura da água.

Development time of embryos in the brood pouch of the cladoceran Penilia avirostris Dana, 1852, was estimated by collecting zooplankton daily for 15 days in surface water of Guanabara Bay, Brazil. Each day the maturity stage of embryos of 90 parthenogenic females was noted. Total development time (egg to birth) varied from 2 to 3 days, the immature phase (stages I to IV) being generally longer (2 days) than intermediate and mature phases (1 day, stages V to XII). Similar results were obtained from Bottrell's equation, which takes water temperature into account.

O objetivo do presente estudo foi registrar alguns aspectos da reprodução das espécies de cladóceros marinhos que ocorrem na Baía de Guanabara. As amostras foram obtidas durante o ano de 1985, com o auxílio de uma rede cônica de 200 mm de diâmetro de poro. As duas espécies identificadas (Penilia avirostris e Pseudevadne tergestina) são comuns à baía, mas desaparecem do plâncton no inverno. Dois meses antes desse fenômeno, as fêmeas partenogenéticas apresentam redução no tamanho da ninhada, enquanto os animais gametogenéticos surgem na população. Essa seqüência de eventos, em geral, é típica para as populações de cladóceros marinhos que ocorrem em regiões temperadas.

The objective of this study was to report on some aspects of marine cladoceran reproduction in Guanabara Bay. Samples were collected during 1985 using a conical net with 200 mm mesh. Two species were identified: Penilia avirostris and Pseudevadne tergestina. Both species disappeared from the plankton in winter. Two months before this phenomenon, parthenogenetic females displayed reduction in brood size, while gamogenetic individuals appeared among the populations. This sequence of events is generally typical of marine cladoceran populations in temperate waters.