ABSTRACT BACKGROUND: Turner syndrome (TS) is a rare genetic disease. Understanding its clinical findings contributes to better management of clinical conditions. OBJECTIVE: To investigate the clinical and karyotypic characteristics of patients diagnosed with TS at two reference services for clinical genetics in southern Brazil. DESIGN AND SETTING: Retrospective cross-sectional study conducted in two clinical genetics services in Porto Alegre (RS), Brazil. METHODS: The sample consisted of 59 patients with TS diagnosed from 1993 to 2019. A review of their medical records was performed and a standard protocol was filled out. RESULTS: The average age of the patients at diagnosis was 15.9 years, and 40.7% were over 13 years old. The largest proportion of them (42.4%) had been referred from an endocrinology department and their constitution was 45,X (40.7%). The most common clinical findings were short stature (85.7%), hypoplastic/ hyperconvex nails (61.2%), low posterior hairline (52.1%) and cubitus valgus (45.8%). There was no difference regarding the presence of short stature (P = 0.5943), number of dysmorphia (P = 0.143), anatomical regions affected and malformations identified through imaging examinations (P = 1.0000), regarding the presence or absence of 45,X constitution. Only 6% of the patients had used growth hormone and 43%, estrogen. CONCLUSION: We found that, in general, patients with TS were being diagnosed late. This has important implications for their treatment. In addition, only a small proportion of the patients were undergoing further examination or evaluation, which appeared to be leading to underdiagnosis of many abnormalities.

ABSTRACT Introduction: Much has been discussed about the training of a plastic surgeon nationally and internationally. There is a need to improve and standardize training to ensure the future of this specialty. Methods: Questionnaires were filled by third year trainees at the Brazilian Congress of Plastic Surgery (Belo Horizonte). Results: A total of 230 questionnaires were distributed and 113 were included in the study. The respondents included 71 men and 41 women; 34 were from institutions recognized by the Brazilian Society of Plastic Surgery (SBCP) and 71 were from institutions recognized by the Ministry of Education and the SBCP. Ninety-six respondents revealed that purely aesthetic procedures were conducted in their institutions, with an average of 54.3% of aesthetic procedures. The respondents had the least confidence in performing hair transplants and the most confidence in performing an abdominoplasty. The topic most requested for training was rhinoplasty and the least requested was abdominoplasty. The trainees were fairly satisfied with their programs, with an average satisfaction level of 3.89, on a scale of 1 to 5. The procedure that needed to be performed more frequently was rhinoplasty (more than 10 procedures). Most trainees felt that the program prepared them to practice surgeries, with an average of 3.8 on a scale of 1 to 5. Further, 65% found it necessary to have a fellowship, with mastology being the most requested. The most common procedure was reduction mammoplasty. Most of the trainees wanted to work in a private clinic. Conclusion: In order to improve the level of education, the accredited institutions should meet the requirements necessary for good preparation of the trainees.

RESUMO Introdução: Muito se discute sobre a formação do cirurgião plástico na especialização médica nacional e internacionalmente. Há necessidade da busca por melhoras e padronização na formação visando o futuro da especialidade. Métodos: Foi avaliado protocolo preenchido no Congresso Brasileiro de Cirurgia Plástica (Belo Horizonte) por especializandos do terceiro ano. Resultados: Foram distribuídos 230 protocolos. 113 protocolos foram incluídos. A amostra incluiu 71 homens e 41 mulheres. 34 eram de serviços cadastrados pela Sociedade Brasileira de Cirurgia Plástica (SBCP) e 71 eram de serviços cadastrados pelo Ministério da Educação e SBCP. 96 afirmaram que em seus serviços são realizados procedimentos puramente estéticos, com média de 54,3% de procedimentos estéticos. O procedimento com menos confiança em realizar foi transplante capilar, e mais confiança foi abdominoplastia. Área de interesse mais requisitada foi rinoplastia e a menos foi abdominoplastia. Os especializandos estão regularmente satisfeitos com seus programas, com média de 3,89, em uma escala de 1 a 5. O procedimento que deve ser mais realizado foi rinoplastia, sendo necessário, do ponto de vista deles, realizar mais de 10 procedimentos. Os especializandos sentem-se bem preparados pela programa, com média de 3,8 em uma escala de 1 a 5. 65% deles acham necessário fazer fellow, sendo o mais requisitado de mastologia. O procedimento mais realizado foi mamoplastia redutora. A maioria dos especializandos quer trabalhar em clínica privada. Conclusão: Visando aprimorar a formação acadêmica, é necessário que os serviços credenciados se adequem aos requisitos necessários para a boa formação dos especializandos.

Abstract Purpose: To evaluate the effects and mechanisms of andiroba-based emulsion (ABE) topical treatment on full-thickness cutaneous wounds in rats. Methods: The wounds were harvested on days 3, 7, 15, and 20 post-surgery. Wound contraction rate, quantitative immunohistochemistry [macrophages, myofibroblasts, capillaries, collagens (col) I and III, transforming growth factor β3β (TGFβ3)], and tensile strength were assessed. Results: Treated wounds were smaller, contracted earlier and had increased angiogenesis, fewer CD68+ and M2 macrophages on days 7 and 15, but higher on day 20. Myofibroblasts appeared on days 3 to 7 in untreated wounds and on days 7 to 15 in treated wounds. TGFβ3 levels were higher in the treated wounds, less dense collagen fibers, lower col I/III ratios and a higher tensile strength. Conclusion: These results demonstrate the important anti-inflammatory role of treatment and the associated modulation of macrophages, myofibroblasts, and TGFβ3 levels. Collagen fibers in the treated wounds were more organized and less dense, similar to unwounded skin, which likely contributed to the higher tensile strength.

Os compostos fenólicos influenciam a qualidade de maçãs e seus produtos tanto em termos de aparência e sabor quanto em propriedades nutricionais. O objetivo deste trabalho foi quantificar os teores de compostos fenólicos totais, antocianinas, flavonóis e atividade antioxidante em maçãs da cultivar Gala e de duas mutações somáticas, Royal Gala e Galaxy Gala, com diferentes intensidades de pigmentação vermelha. Os teores de fenóis totais foram semelhantes nas frutas inteiras. A atividade antioxidante apresentou diferença significativa na fruta inteira, sendo que a cultivar Gala foi a que obteve maior valor (17559 µm.g-1) e a cv. Galaxy Gala, com pouca pigmentação, o menor (6160 µg.g-1). A cultivar Royal Gala apresentou valores intermediários de atividade antioxidante de 12050 µm.g-1, para as frutas com muita pigmentação vermelha, e 13546 µm.g-1 para as com pouca pigmentação. O teor de antocianinas na fruta inteira variou de 5,79 a 13,73 mg.100 g-1 e o teor de flavonóis oscilou entre 23,37 e 382,83 mg.100 g-1 nas cvs. Royal Gala e Galaxy Gala, respectivamente. Analisado nas diferentes partes das maçãs, o epicarpo apresentou a maior concentração de fenóis com aproximadamente 57% do conteúdo total e 42% da atividade antioxidante. Os compostos fenólicos e a atividade antioxidante nos sucos oxidados foram significativamente reduzidos com o escurecimento enzimático, com perdas de 69% e 83%, respectivamente. As frutas de mesma cultivar com maior e menor intensidade de cor apresentaram teores de compostos fenólicos e capacidade antioxidante semelhantes, porém as cultivares variaram significativamente.

Phenolic compounds influence the quality of apples and their products in appearance, flavor, and nutritional properties. The aim of this work was to quantify the phenolic compounds, anthocyanins, flavonols, and antioxidant activity in Gala apples and of two somatic mutations, Royal Gala and Galaxy Gala, with different intensities of red pigmentation. The total phenolic content was similar in these fruits. The antioxidant activity presented significant difference in the whole fruits. gala variety presented the highest value (17559 µm.g-1) while Galaxy Gala, with low pigmentation, presented the lowest value (6160 µm.g-1). The anthocyanins content in the whole fruit varied from 5.79 to 13.73 mg.100 g-1 and the flavonols content from 23.37 to 382.83 mg.100 g-1 in Royal Gala and Galaxy Gala, respectively. The phenolic compounds presented the highest concentration in the epicarp, around 57% of the total content, and 42% of the antioxidant activity. The phenolic compounds and the antioxidant activity in the apple juices were significantly reduced with the enzymatic browning, with losses of 69% and 83% respectively. The fruits from the same variety with high and low color intensity showed similar values of phenolic compounds content and antioxidant capacity. However, the cultivars varied significantly.