Abstract The present study evaluated the actual rotational speed of three different endodontic motors compared to the values provided by the manufacturers. A total of three endodontic motors (X-Smart Plus, VDW.Silver, and iRoot) were tested at 400 rpm and 800 rpm and 2 N/cm2 torque. The kinematics of the devices was recorded by using a custom angle-measuring disc with a 50-mm diameter attached to the handpiece provided by the manufacturer, whereas their movement was captured by a high-speed camera at 2,400 frames per second, 800 x 800 pixel-resolution and distance of 0.3 m from the target object. Statistical analysis was performed at a significance level of 5%. At 400 rpm, the iRoot motor had a value of 17.94 rpm above that indicated by the manufacturer, which was significantly different from those of X-Smart Plus (5.20 rpm below that indicated by the manufacturer) and VDW.Silver (0.62 rpm above that indicated by the manufacturer) motors (P < 0.05). At 800 rpm, the iRoot motor had a value of 51.34 rpm below that indicated by the manufacturer, whereas the X-Smart Plus motor had a value of 13.00 rpm below that indicated by the manufacturer (P > 0.05). The VDW.Silver motor statistically differed from the iRoot and X-Smart Plus ones, showing a value of 1.68 rpm above that indicated by the manufacturer. In conclusion, the X-Smart Plus, VDW.Silver, and iRoot motors showed lower variations in the rotational speed values compared to those reported by their manufacturers. The endodontic motors presented different behaviors between them, with the VDW.Silver motor presents the most accurate values and the iRoot presents the most divergent values.

Resumo O presente estudo avaliou a real velocidade rotacional de três diferentes motores endodônticos comparados aos valores fornecidos pelos fabricantes. Um total de três motores endodônticos (X-Smart Plus, VDW.Silver e iRoot) foram testados a 400 rpm e 800 rpm e torque de 2 N/cm2. A cinemática dos dispositivos foi verificada utilizando-se um disco customizado de 50 mm de diâmetro acoplado ao contra ângulo fornecido pelo fabricante e o movimento foi capturado através de uma câmera ajustada em 2400 quadros por segundo com resolução de 800 x 800 pixels distante 0,3 m do objeto alvo. A análise estatística foi realizada com nível de significância de 5%. A 400 rpm, o motor iRoot apresentou 17,94 rpm acima do indicado pelo fabricante, sendo o valor significativamente diferente do X-Smart Plus (5,20 rpm abaixo do indicado pelo fabricante) e VDW.Silver (0.62 rpm acima do indicado pelo fabricante) (P > 0,05). A 800 rpm, iRoot apresentou 51.34 rpm abaixo do indicado pelo fabricante e X-Smart Plus 13,00 rpm abaixo do indicado pelo fabricante (P > 0,05). VDW.Silver foi estatisticamente diferente de iRoot e X-Smart Plus, apresentando 1,68 rpm acima do indicado pelo fabricante. Concluiu-se que os motores X-Smart Plus, VDW.Silver e iRoot apresentaram baixa variação na velocidade rotacional quando comparada ao fornecido pelo fabricante. Os motores endodônticos apresentaram diferentes comportamentos entre eles, sendo que o motor VDW.Silver apresentou valores mais acurados enquanto o iRoot foi o mais divergente.

The aim of this work was to identify and quantify the metabolites present in the roots of Combretum lanceolatum inoculated with its endophytic fungi. The metabolomics was accomplished using the 1H nuclear magnetic resonance (NMR) spectral data and evaluated via rNMR software and Madison Metabolomics Consortium Database (MMCD). The principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) showed that plants inoculated with Trichoderma spirale (Ts) present differentiation and discrimination over the time compared to control. Seven days after Ts fungal inoculation, 15 metabolites were identified at different concentrations comparing to the control plants. The plants inoculated with Ts fungus present the metabolites spermidine and pantothenate in higher concentrations and 3-hydroxybutyric acid and b-alanine in lower concentrations compared to control plants, indicating any response to biotic stress. These metabolites are involved in various plant processes, including secondary metabolites biosynthesis, energy metabolism and self-defense. Therefore, this work demonstrates the diversification of primary metabolites composition influenced by endophytes inoculation.

ABSTRACT Soil compaction negatively impacts soil physical functions, affecting root growth and crop yield. This study aimed to evaluate different soybean cultivars’ agronomic and physiological performance as a function of compaction in Rhodic Ferralsol under no-tillage (NT) with a clay loam texture in the Brazilian Cerrado biome. The experiment was implemented in Rio Verde, Goias state, central Brazil. The experimental design was a randomized block with a 3x2 factorial scheme with six replications. The first factor corresponded to three soybean cultivars from different maturity groups (MG) identified as super-early (MG = 6.2); early (MG = 6.8), and medium (MG = 7.9). The second factor was the cultivation in compacted and uncompacted soil. The experiment evaluated the soil physical properties: bulk density, total porosity, water content at matric potential -6 kPa, aeration capacity and water and air holding capacity in 0.0-0.1 and 0.1-0.2 m layers, agronomic and physiological traits (gas exchange). Soil compaction negatively affected the agronomic traits of soybean cultivars, with better performance of the medium cycle cultivar. Net photosynthesis, stomatal conductance, and transpiration rates were reduced up to 50% under compaction and were not influenced by cultivars. Our findings suggest that soil compaction negatively affected the performance of soybean cultivars with lower maturity groups reflecting in lower plant height, shoot and root dry mass, number of pods and grain yield. The choice of soybean cultivars with a longer cycle can be an alternative to minimize the adverse effects of soil compaction.

BACKGROUND Leishmaniasis, a neglected disease caused by the parasite Leishmania, is treated with drugs associated with high toxicity and limited efficacy, in addition to constant reports of the emergence of resistant parasites. In this context, snake serums emerge as good candidates since they are natural sources with the potential to yield novel drugs. OBJECTIVES We aimed to show the antileishmanial effects of γCdcPLI, a phospholipase A2 inhibitor from Crotalus durissus collilineatus snake serum, against Leishmania (Leishmania) amazonensis. METHODS Promastigotes forms were exposed to γCdcPLI, and we assessed the parasite viability and cell cycle, as well as invasion and proliferation assays. FINDINGS Despite the low cytotoxicity effect on macrophages, our data indicate that γCdcPLI has a direct effect on parasites promoting an arrest in the G1 phase and reduction in the G2/M phase at the highest dose tested. Moreover, this PLA2 inhibitor reduced the parasite infectivity when promastigotes were pre-treated. Also, we demonstrated that the γCdcPLI treatment modulated the host cell environment impairing early and late steps of the parasitism. MAIN CONCLUSIONS γCdcPLI is an interesting tool for the discovery of new essential targets on the parasite, as well as an alternative compound to improve the effectiveness of the leishmaniasis treatment. Leishmaniasis efficacy context A serum (Leishmania amazonensis cycle assays macrophages G G2M GM G2 M tested Moreover PLA pretreated. pretreated pre treated. pre-treated Also parasitism

A BRIEF OVERVIEW ON SINGLE-ATOM CATALYSIS: CONCEPTS AND APPLICATIONS. Catalytic processes became extremely important for the development of our society, especially after the second industrial revolution. Thus, the research for more efficient catalysts is an obstacle to overcome to achieve cheaper processes, higher yields, and selectivity of desired products. In this context, single-atom catalysis emerges as a promising alternative to unite the advantages of traditional homogeneous and heterogeneous catalysis. Catalysis by single-atoms is a bridge that unites in a single catalyst the ease of recovery and reuse (from heterogeneous catalysis) with the high exposure and uniformity of sites (from homogeneous catalysis). Thus, single-atom catalysts (SACs) and single-atom alloys (SAAs) have already found several applications in the literature, such as in hydrogenation, oxidation, conversion of biomass derivatives, electrocatalysis, and photocatalysis. However, it is essential to emphasize that it is still a field that is expanding and relatively new, with several opportunities and many barriers to surpass. In this review, concepts of homogeneous, enzymatic, and heterogeneous catalysis will be addressed, as well as fundamental aspects of single-atom catalysis, preparation methods, characterization, and current challenges.

Abstract Caves are habitats characterized by low light or total darkness, whose fauna is largely composed of animals that adapt to dark environments, such as bats and many arthropods. Here, we present the first record of bat carcass colonization by Diptera larvae in a cave in the Neotropics. Twenty-one adult specimens of Microcerella halli (Sarcophagidae), seven males and 14 females, emerged from larvae collected in a carcass of Pteronotus gymnonotus (Mormoopidae) found in a twilight zone, about 15 m from the cave entrance. The mean time between carcass collection and adult emergence was 17.99 ± 0.44 days. As colonization by M. halli only occurs after death, we estimated the mean duration of the pupal stage - by recording daily pupation and emergence data - and attempted a preliminary estimate of the post-mortem interval, which indicated that larviposition occurred at least 24 h before carcass collection. Furthermore, we discussed the fact that M. halli females can find and colonize carcasses in low light environments, reinforcing the forensic potential of the species.

Resumo O bioma das Florestas e Arbustais Tropicais Sazonalmente Secos tem na Caatinga sua mais extensa área, possuindo elevada diversidade florística e ambiental. Caracterizamos a composição florística e a fisionomia da vegetação no Refúgio da Vida Silvestre Pedra da Andorinha (RPA), área de conservação no noroeste do estado do Ceará, Brasil. Realizamos coletas botânicas entre março de 2015 e maio de 2021, aplicando os métodos tradicionais de coleta e herborização. Todo o material foi depositado no herbário HUVA. A fitossociologia foi feita em cinco parcelas de 50 x 50 m (1,25 ha), onde os indivíduos lenhosos com diâmetro ao nível do solo ³ a 3 cm foram inventariados. Avaliamos os parâmetros fitossociológicos de estrutura da vegetação e sua diversidade florística. Identificamos 266 plantas vasculares, distribuídas em 185 gêneros e 67 famílias, incluindo uma pteridófita (Marsilea deflexa - Marsileaceae). A família Fabaceae teve a maior riqueza (38 spp.), enquanto Ipomoea foi o gênero mais rico (9 spp.). O componente herbáceo foi o componente mais rico (43,6%), com uma predominância de herbáceas terófitas (57,5% de todas as plantas herbáceas). O estudo fitossociológico amostrou 1.988 indivíduos, distribuídos em 24 espécies de 13 famílias. As espécies com maior valor de importância foram Cordia oncocalyx e Croton blanchetianus, responsáveis por 63% dos indivíduos amostrados. Classificamos a área como Caatinga sensu stricto e em vegetação rupícola de inselbergues e afloramentos rochosos, chamando a atenção para a elevada riqueza de espécies no componente herbáceo, que, como em outras áreas de Caatinga sensu stricto, supera a riqueza de espécies lenhosas.

Abstract The Seasonally Dry Tropical Forests and Woodlands biome (SDTFW) has its largest nucleus in the Caatinga domain. We characterized the flora and physiognomy of the vegetation in the Pedra da Andorinha Wildlife Refuge (RPA), in Ceará, Brazil. Plant collections were made between March/2015 and May/2021, applying usual botanical methods. All material was deposited in HUVA herbarium. The phytosociological studies covered five 50 m x 50 m plots established (1.25 ha) in which all individuals with diameters at soil level ³ 3 cm were inventoried. We identified 266 vascular plants species distributed among 185 genera and 67 families, including one fern (Marsilea deflexa - Marsileaceae). Fabaceae had the greatest species richness (38 spp.), while Ipomoea was the richest genus (9 spp.). 43.6% of all plant species were herbaceous, with a predominance of therophytes (57.5% of all herbaceous plants). The phytosociological study sampled 1,988 individuals distributed among 24 species of 13 families. The species with the greatest important value were Cordia oncocalyx (Boraginaceae) and Croton blanchetianus (Euphorbiaceae). We classify the local physiognomy as typical caatinga sensu stricto vegetation and rocky vegetation on inselbergs and outcrops. We highlight the richness of herbaceous plants in the local community, which surpass the richness of the woody component.

RESUMO Objetivo: integrar evidências científicas sobre o perfil de habilidades linguísticas na dislexia do desenvolvimento e no transtorno do desenvolvimento da linguagem, para melhor compreensão de possíveis condições comórbidas ou quadros isolados. Métodos: trata-se de uma revisão integrativa da literatura. A busca ocorreu nas bases de dados: Scielo, Eric e LILACS, bem como no buscador PubMed. Os critérios de inclusão foram: 1) estudos publicados nos periódicos nacionais e internacionais, gratuitos, com texto completo e disponível; 2) que abordem a temática nos últimos 10 anos; 3) em inglês e/ou português. Revisão da Literatura: a busca contou com 654 artigos. A amostra final foi composta por dez artigos, nos quais cinco estudos são concordantes quanto à hipótese de que dislexia e transtorno do desenvolvimento da linguagem, podem ser transtornos comórbidos, com prejuízos na leitura, na escrita e na consciência fonológica; os demais estudos descrevem déficits mais amplos de linguagem nas crianças somente com transtorno do desenvolvimento da linguagem, implicados na decodificação e compreensão da linguagem, enquanto na dislexia os déficits estão associados ao processamento fonológico. Conclusão: não há consenso se a dislexia e o transtorno do desenvolvimento da linguagem ocorrem simultaneamente em crianças. No entanto, ambos conferem riscos à compreensão leitora e ao desempenho escolar.

ABSTRACT Purpose: to gather scientific evidence on the linguistic skill profile in developmental dyslexia and developmental language disorder to better understand possible comorbid or isolated conditions. Methods: an integrative review of the literature. The search was conducted in SciELO, ERIC, LILACS, and PubMed. The inclusion criteria were as follows: 1) studies published and available in open-access national and international journals in full text; 2) addressing the topic in the last 10 years; 3) in English and/or Portuguese. Literature Review: the search retrieved 654 articles, the final sample comprising 10, 5 of which agreed with the hypothesis that dyslexia and developmental language disorder can be comorbid disorders, impairing reading, writing, and phonological awareness. The other studies described broader language deficits in children with developmental language disorder alone, affecting language decoding and comprehension, whereas deficits in dyslexia are more associated with phonological processing. Conclusion: there is no consensus on whether dyslexia and developmental language disorders occur simultaneously in children. However, both pose risks to reading comprehension and school performance.

Resumen El objetivo de este trabajo es estudiar la viabilidad del empleo de Drones para mapear e inspeccionar manifestaciones patológicas en pavimentos asfálticos en comparación con el método tradicional. Se establecieron parcelas muestrales cada 20 m, con 6 m de extensión, totalizando 20 estaciones, las cuales tuvieron sus manifestaciones patológicas anotadas y calculado el Índice Global de Gravedad para el área de estudio. Los resultados obtenidos con las metodologías de referencia y alternativa se confrontaron en función de las patologías identificadas y el tiempo empleado en los levantamientos. Los resultados demostraron viabilidad para el empleo de Drones en la inspección de patologías de pavimentos asfálticos, tomando como base la Norma DNIT 006/2003 - PRO, obteniendo resultados similares a la metodología tradicional con reducción significativa del tiempo de operación (33,3%).

Abstract This work objective is to study the feasibility of the Drones’ use for mapping and inspecting pathological manifestations in asphalt pavements compared to the traditional method. Sampling plots were established every 20 m, with 6 m extension, making a total of 20 stations, which had their pathological manifestations recorded and calculated with the Global Severity Index for the study area. The results obtained by the reference and alternative methodologies were confronted according to the pathologies identified and the time spent in the surveys. The results showed feasibility in the use of drones for inspection of pathologies in asphalt pavements, based on DNIT 006/2003 - PRO, obtaining results similar to those of the traditional methodology with a significant reduction of the time of operation (33.3%).

Resumo Este trabalho objetiva estudar a viabilidade do emprego de Drones para mapeamento e inspeção de manifestações patológicas em pavimentos asfálticos comparando ao método tradicional. Foram estabelecidas parcelas amostrais a cada 20 m, com 6 m de extensão, totalizando 20 estações, as quais tiveram suas manifestações patológicas anotadas e calculado o Índice Global de Gravidade para a área de estudo. Os resultados obtidos pelas metodologias de referência e alternativa foram confrontados em função das patologias identificadas e do tempo gasto nos levantamentos. Os resultados demonstraram viabilidade para emprego de Drones para inspeção de patologias em pavimentos asfálticos, tomando como base a Norma DNIT 006/2003 - PRO, obtendo resultados similares à metodologia tradicional com redução significativa do tempo de operação (33,3%).

ABSTRACT Increase on intensive monocultures is an important threat to Caatinga biodiversity decrease. The use of chemical insecticides directly affects insect natural enemies of crops, but also non-target species. We aimed to test whether the diversity of sarcosaprophagous flies would be affected by agricultural management (conventional and organic). Adult dipterans were collected monthly, from July 2015 to January 2016, using a grid composed of 12 suspended traps distant 20 m from each other, baited with 150 g of sardine or bovine spleen, in each type of management. We first calculated α-diversity by Hill numbers, then evaluated the difference of diversity metrics by ANOVA. We also graphically visualise the species composition distribution by NMDS. We recorded a total of 4,651 specimens of 15 Calliphoridae and Sarcophagidae species. Chrysomya spp. were the dominant species (93.7% of all blowflies). Sarcophagidae species were sampled in low abundance, but with exclusive presence of some species in each treatment (e.g. B laesoxipha stallengi and Ravinia belforti in organic management). Although there was no difference on sarcosaprophagous fly diversity between treatments, we demonstrate empirically that mango plantations in the Caatinga harbour diverse and abundant assemblages of sarcosaprophagous dipterans, whose richness of species is comparable to other ecosystems such as rainforests and urban areas. Our results expand the known geographical range for forensically relevant species by providing a checklist of sarcosaprophagous dipterans that can thrive in agroecosystems in the Caatinga and, potentially, colonize cadavers disposed in that type of environment.

Abstract One of the major health security challenges of the 21st century is the occurrence of microbial infections and bacterial complications that could affect 10 million people by 2050. On the biomaterial field, implant metallic currently replaces partial or total body parts and can fail to be integrated into the body due to infections. This study performs two combined surface modifications on Ti-30Ta alloy, in order to obtain an infection-resistance and osseointegration surface on metallic implants to be tested within bacterial biofilm. The Group 1 investigated surface modifications by the anodization process in the electrolyte glycerol + NH4F 0.25% at 30V- 9 hours and annealed in 530°C (5°C/min). The Group 2 underwent the same process as Group 1 and, additionally, the samples were immersed in 0.3 M CaCl2 and 0.5 M Na2HPO4 solutions for hydroxyapatite growth. The substrate was characterized using scanning electron microscopy (SEM), X-ray diffractometer (XRD) and dynamic contact angle. S. epidermidis bacterial adhesion and biofilm formation. The results indicated that the Group 1 shows a higher antimicrobial activity, hydrophilic behavior and potential to be used for metallic implant applications. The Group 2 with the hydroxyapatite film coating did not have an improvement in the antimicrobial response.

RESUMO OBJETIVO Descrever a evolução do consumo de alimentos fora do domicílio no Brasil no período 2008–2018. MÉTODOS Foram usados dados dos Inquéritos Nacionais de Alimentação (INA) de 2008–2009 e 2017–2018, realizados com 34.003 e 46.164 indivíduos, respectivamente, para estimar a frequência de consumo de alimentos fora de casa e a contribuição desse consumo para alimentos específicos. O consumo de alimentos foi investigado por meio de registros alimentares no INA 2008–2009 e de recordatórios 24 horas em 2017–2018. As estimativas foram geradas para o Brasil como um todo, para áreas urbana e rural, para faixas de idade (adolescente, adulto, idoso) e para faixas de renda. RESULTADOS A frequência de consumo fora de casa diminuiu 8,8% entre os dois inquéritos, sem alteração na área rural, nas regiões Nordeste e Sul e nas menores faixas de renda. Houve leve aumento entre idosos e na região Centro-Oeste. De modo geral, a contribuição do consumo alimentar fora de casa para a ingestão diária de energia também diminuiu (16,3% vs. 12,7%), com exceção da área rural, onde houve redução da diferença em relação à área urbana entre os dois inquéritos. O consumo de alimentos fora de casa foi reduzido para a maioria dos itens avaliados. Os alimentos mais consumidos fora de casa em ambos os inquéritos foram as bebidas alcoólicas, salgadinhos fritos e assados, refrigerantes, pizzas, doces e sanduíches. CONCLUSÃO Em 10 anos, a prevalência de consumo de alimentos e o percentual de contribuição dos alimentos consumidos fora de casa diminuíram no Brasil, mas os alimentos ultraprocessados ainda figuram como o grupo de alimento mais consumido fora de casa.

ABSTRACT OBJECTIVE To describe the evolution of out-of-home food consumption in Brazil in 2008–2018. METHODS We used the 2008–2009 and 2017–2018 data from the Inquéritos Nacionais de Alimentação (INA - National Food Surveys), conducted amid 34,003 and 46,164 individuals, to estimate the frequency of out-of-home food consumption and the contribution of this consumption to specific foods. Food consumption was analyzed using food records in the 2008–2009 INA and 24-hour recalls in 2017–2018. Estimates were generated for Brazil in general, for urban and rural areas, for age groups (adolescent, adult, elderly), and for income bracket. RESULTS The frequency of out-of-home consumption decreased by 8.8% between the two surveys, with no change in the rural area, in the Northeast and South regions, and for the lowest income brackets. We observed a slight increase among the elderly and in the Midwest region. The contribution of out-of-home food consumption to daily energy intake also decreased (16.3% vs. 12.7%), excepting the rural area, where there was a reduction in the difference in relation to the urban area between the two surveys. For most items evaluated, the out-of-home food consumption decreased. The most consumed out-of-home food were alcoholic beverages, fried and baked snacks, soft drinks, pizza, sweets, and sandwiches in both surveys. CONCLUSION In 10 years, the prevalence of food consumption and the percentage of contribution of out-of-home food decreased in Brazil, but ultra-processed foods still figure as the most consumed food group outside the home.

RESUMO OBJETIVO Descrever a evolução do consumo de alimentos fora do domicílio no Brasil no período 2008–2018. MÉTODOS Foram usados dados dos Inquéritos Nacionais de Alimentação (INA) de 2008–2009 e 2017–2018, realizados com 34.003 e 46.164 indivíduos, respectivamente, para estimar a frequência de consumo de alimentos fora de casa e a contribuição desse consumo para alimentos específicos. O consumo de alimentos foi investigado por meio de registros alimentares no INA 2008–2009 e de recordatórios 24 horas em 2017–2018. As estimativas foram geradas para o Brasil como um todo, para áreas urbana e rural, para faixas de idade (adolescente, adulto, idoso) e para faixas de renda. RESULTADOS A frequência de consumo fora de casa diminuiu 8,8% entre os dois inquéritos, sem alteração na área rural, nas regiões Nordeste e Sul e nas menores faixas de renda. Houve leve aumento entre idosos e na região Centro-Oeste. De modo geral, a contribuição do consumo alimentar fora de casa para a ingestão diária de energia também diminuiu (16,3% vs. 12,7%), com exceção da área rural, onde houve redução da diferença em relação à área urbana entre os dois inquéritos. O consumo de alimentos fora de casa foi reduzido para a maioria dos itens avaliados. Os alimentos mais consumidos fora de casa em ambos os inquéritos foram as bebidas alcoólicas, salgadinhos fritos e assados, refrigerantes, pizzas, doces e sanduíches. CONCLUSÃO Em 10 anos, a prevalência de consumo de alimentos e o percentual de contribuição dos alimentos consumidos fora de casa diminuíram no Brasil, mas os alimentos ultraprocessados ainda figuram como o grupo de alimento mais consumido fora de casa.

ABSTRACT OBJECTIVE To describe the evolution of out-of-home food consumption in Brazil in 2008–2018. METHODS We used the 2008–2009 and 2017–2018 data from the Inquéritos Nacionais de Alimentação (INA - National Food Surveys), conducted amid 34,003 and 46,164 individuals, to estimate the frequency of out-of-home food consumption and the contribution of this consumption to specific foods. Food consumption was analyzed using food records in the 2008–2009 INA and 24-hour recalls in 2017–2018. Estimates were generated for Brazil in general, for urban and rural areas, for age groups (adolescent, adult, elderly), and for income bracket. RESULTS The frequency of out-of-home consumption decreased by 8.8% between the two surveys, with no change in the rural area, in the Northeast and South regions, and for the lowest income brackets. We observed a slight increase among the elderly and in the Midwest region. The contribution of out-of-home food consumption to daily energy intake also decreased (16.3% vs. 12.7%), excepting the rural area, where there was a reduction in the difference in relation to the urban area between the two surveys. For most items evaluated, the out-of-home food consumption decreased. The most consumed out-of-home food were alcoholic beverages, fried and baked snacks, soft drinks, pizza, sweets, and sandwiches in both surveys. CONCLUSION In 10 years, the prevalence of food consumption and the percentage of contribution of out-of-home food decreased in Brazil, but ultra-processed foods still figure as the most consumed food group outside the home.

RESUMO Os remotely piloted aircrafts - RPAs, estão firmados no mercado como poderosa ferramenta de aquisição de imagens aéreas, facilitando os mapeamentos para diversos fins. Assim, objetivou-se avaliar a qualidade dos processos a partir da geração de produtos digitais georreferenciados com diferentes quantidades de pontos de controle (GCPs), utilizando Controle Estatístico de Processos (CEP) e Padrão de Exatidão Cartográfico (MAS) em ortomosaico produzido com imagens de RPA. Foi utilizado um RPA multirrotor para aquisição de imagens aéreas em área de 2 hectares. Posteriormente foi gerado ortomosaico por meio do software PhotoScan, georreferenciado com oito, cinco e três GCPs (Pontos de controles). Os erros de posicionamento foram submetidos ao CEP para avaliar a qualidade dos processos e os ortomosaicos foram qualificados pelo MAS. Os resultados são promissores, tendo em vista os erros de posicionamento menores que 0,1 m nos ortomosaicos gerados, classificados como padrão “A” de Exatidão Cartográfica. Os Controles Estatísticos de Processos demonstraram níveis aceitáveis de erros, indicando grande acurácia para levantamentos desta natureza. As elevadas precisões obtidas nos mapeamentos indicam que imagens aéreas obtidas por meio de RPAs podem ser utilizadas em levantamentos topográficos, contanto que se respeitem os padrões de erros e controle de processos, atestando a qualidade dos resultados.

ABSTRACT Remotely piloted aircraft (RPA) are established in the market as a powerful tool for acquiring aerial images and facilitating mapping for various purposes. The aim of this study was to evaluate the quality of processes originating from the generation of georeferenced digital products employing a differing number of ground control points (GCP), using Statistical Process Control (SPC) and Mapping Accuracy Standards (MAS) in an orthomosaic produced with images from an RPA. A multirotor RPA was used to acquire aerial images over an area of 2 hectares. An orthomosaic was later generated using the PhotoScan software, and georeferenced with eight, five and three GCP (ground control points). Positioning errors were submitted to SPC to evaluate the quality of each process, and the orthomosaics were qualified by MAS. The results are promising, in view of the positioning errors of less than 0.1 m in the generated orthomosaics, which are classified as Mapping Accuracy Standards class ‘A’. Statistical Process Control showed acceptable levels of error, indicating the high accuracy of surveys of this nature. The precision obtained when mapping shows that aerial images obtained by means of RPA can be used in topographic surveys as long as error standards and process control are observed, attesting to the quality of the results.

Resumo Fundamento: As pessoas que vivem com HIV têm um risco aumentado de doença cardiovascular e espessamento da carótida, devido à inflamação causada pelo vírus, à terapia antirretroviral e a outros fatores de risco. No entanto, poucos estudos observaram a ocorrência de doenças cardiovasculares e espessamento carotídeo na população soropositiva com baixo risco cardiovascular e carga viral indetectável. Objetivos: Avaliar a associação entre níveis de marcadores inflamatórios e espessura da carótida em pessoas vivendo com HIV, sob terapia antirretroviral e com baixo risco cardiovascular. Métodos: Para determinar o baixo risco cardiovascular em ambos os grupos (indivíduos infectados e não-infectados pelo HIV), foi utilizado o Escore de Risco de Framingham. Os marcadores inflamatórios (IFN-γ, TNF-α, IL-1β, IL-6, sVCAM-1 e sICAM-1) foram avaliados por citometria de fluxo. A espessura da carótida (mm) foi mensurada por meio de ultrassom com Doppler. O nível de significância foi de p < 0,05. Resultados: Em pessoas vivendo com HIV, a idade e o tabagismo foram associados a alterações da espessura da carótida. No grupo não-HIV, idade e níveis mais altos de colesterol total e LDL foram associados ao aumento da espessura da carótida. Utilizando a análise multivariada, observou-se associação significativa entre os níveis de TNF-α e IL-1β e maior chance de desenvolvimento de aterosclerose no grupo com HIV. Conclusão: Ambos os grupos têm risco semelhante de desenvolver doença cardiovascular, portanto, nosso estudo demonstra que indivíduos HIV-positivos com carga viral indetectável em terapia antirretroviral sem inibidores de protease e com baixo risco cardiovascular não apresentam diferenças na espessura da carótida em relação aos indivíduos não-infectados.

Abstract Background: People living with HIV are at increased risk of cardiovascular disease and carotid thickness, due to the inflammation caused by the virus, the antiretroviral therapy, and other risk factors. However, few studies have observed the occurrence of cardiovascular diseases and carotid thickness in HIV-positive population at low cardiovascular risk and with undetectable viral load. Objectives: To evaluate the association between levels of inflammatory markers and carotid thickness in people living with HIV, under antiretroviral therapy and at low cardiovascular risk. Methods: To determine low cardiovascular risk in both groups (HIV infected and non-infected individuals), the Framingham Risk Score was used. Inflammatory markers (IFN-γ, TNF-α, IL-1β, IL-6, sVCAM-1, and sICAM-1) were assessed using flow cytometry. Carotid thickness (mm) was measured using Doppler ultrasound. Level of significance was p < 0.05. Results: In People living with HIV, age and smoking status were associated with carotid thickness alterations. In the non-HIV group, age, higher total cholesterol, and LDL levels were associated with increased carotid thickness. Using the multivariate analysis, a significant association between TNF-α and IL- 1( levels, and a higher chance of atherosclerosis development in HIV group were observed. Conclusions: Both groups have a similar risk for developing cardiovascular disease, therefore our study demonstrates that HIV-positive individuals with undetectable viral load in antiretroviral therapy without protease inhibitors and with low cardiovascular risk do not present differences in carotid thickness in relation to uninfected individuals.