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Abstract The sexual division of labor causes inequality between genders, as well as situations of violence directed to women in the workplace. In the pharmaceutical area, the majority of women and insertion in multiple scenarios, involving contact with public and differentiated professional partnerships, enhance exposure to violence. Thus, the objective of this study was to analyze situations of violence experienced by pharmacists in the workplace. For this, a qualitative method was used, with content analysis, performed in the NVivo® software, of an online survey, answered by pharmacists (n=381) registered in the Regional Pharmacy Council of Minas Gerais, Brazil. As a result, four categories were identified: ‘Living with disrespect, threats and vulnerability,’ ‘Sexism and structural chauvinism that shuts down, diminishes and torments,’ ‘Discrimination against women as an obstacle to equity’ and ‘Sexual harassment and objectification of women.’ Several expressions of violence in the labor environment emerged from multiple aggressors. Pharmacists recognized professional impairment and little recognition for technical capacity. We concluded that the reports exposed the lack of protection of pharmaceutical integrity in the workplace. It is expected that these results bring to light the gender inequality in pharmaceutical work, with emphasis on situations of violence, expanding the discussion and providing evolution of this mostly female profession.

Resumen La división sexual del trabajo ocasiona iniquidad entre los géneros, así como situaciones de violencia dirigidas a las mujeres en el entorno laboral. En el ámbito farmacéutico, la mayoría de las mujeres y la inserción en múltiples escenarios, que implican contacto con el público y asociaciones profesionales diferenciadas, potencian la exposición a la violencia. Por lo tanto, el objetivo de este estudio fue analizar las situaciones de violencia experimentadas por las farmacéuticas en el entorno laboral. Para esto, se utilizó un método cualitativo, con análisis de contenido, realizado en el software NVivo®, de una encuesta en línea, respondida por farmacéuticas (n=381) registradas en el Consejo Regional de Farmacia de Minas Gerais, Brasil. A consecuencia, se identificaron cuatro categorías: ‘Conviviendo con falta de respeto, amenazas y vulnerabilidad’, ‘el sexismo y el machismo estructural que cala, disminuye y atormentó’, ‘la discriminación contra la mujer como obstáculo a la equidad’ y ‘el acoso sexual y la objeción de la mujer’. Han surgido varias expresiones de violencia en el entorno laboral procedentes de múltiples agresores. Los productos farmacéuticos han reconocido el daño profesional y el escaso reconocimiento de la capacidad técnica. Se concluye que los informes haciendo hincapié de manifiesto la falta de protección de la integridad farmacéutica en el entorno laboral. Se espera que estos resultados traigan a la luz la desigualdad de género en el trabajo farmacéutico, haciendo hincapié en las situaciones de violencia, ampliando el debate y dando lugar a la evolución de esa profesión principalmente femenina.

Resumo A divisão sexual do trabalho ocasiona iniquidade entre gêneros, bem como situações de violência direcionadas às mulheres no ambiente laboral. Na área farmacêutica, a maioria feminina e a inserção em múltiplos cenários, que envolvem contato com público e parcerias profissionais diferenciadas, potencializam a exposição à violência. Assim, o objetivo deste estudo foi analisar situações de violência vivenciadas por farmacêuticas no ambiente laboral. Para tal, utilizou-se método qualitativo, com análise de conteúdo, realizada no software NVivo®, de uma survey on line, respondida por farmacêuticas (n=381) registradas no Conselho Regional de Farmácia de Minas Gerais, Brasil. Como resultado, identificou-se quatro categorias: ‘Convivendo com o desrespeito, ameaças e vulnerabilidade’, ‘O sexismo e o machismo estrutural que cala, diminui e atordoa’, ‘A discriminação contra a mulher como obstáculo à equidade’ e ‘Assédio sexual e objetificação da mulher’. Emergiram diversas expressões de violências no ambiente laboral provenientes de múltiplos agressores. Farmacêuticas reconheceram prejuízo profissional e pouco reconhecimento pela capacidade técnica. Conclui-se que os relatos expuseram a falta de proteção da integridade farmacêutica no ambiente de trabalho. Espera-se que esses resultados tragam à luz a desigualdade de gênero no trabalho farmacêutico, com destaque para situações de violência, ampliando a discussão e proporcionando evolução dessa profissão majoritariamente feminina.

https://doi.org/10.1590/1981-7746-ojs2154

Circulating microparticles and thrombin generation in patients with Chronic Lymphocytic Leukemia
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Evangelista, Fernanda Cristina Gontijo

; Ferrão, Aline Lúcia Menezes

; Duarte, Rita Carolina Figueiredo

; Gomes, Lorena Caixeta

; Alves, Luan Carlos Vieira

; Campos, Fernanda Magalhães Freire

; Braga, Tatiane Vieira

; Santiago, Marie Gabriele

; Araújo, Sergio Schusterschitz da Silva

; Carvalho, Maria das Graças

; Sabino, Adriano de Paula

Brazilian Journal of Pharmaceutical Sciences

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2022, Volume 58 elocation e19407

Abstract: En | Text: En | PDF: En

Abstract Chronic Lymphocytic Leukemia (CLL) has shown great biological heterogeneity, with a variable prognosis, and a short survival in some patients. In this light, the present study focused on the prognostic utility of circulating microparticles (MPs) and a Thrombin Generation (TG) Profile for thrombotic risk and disease progression in CLL patients. Circulating microparticles and TG were evaluated in 35 patients with CLL and 35 healthy individuals. For circulating microparticles, significant differences were observed among the following groups: MPs derived from endothelial cells (p = 0.002), B lymphocytes (p < 0.001), platelets (p = 0.003), and Tissue Factor MPs in monocytes (p < 0.001). In all cases, MP values were higher for the CLL group. When compared to the controls, CLL patients presented a decrease in TG, characterized by a reduced endogen thrombin potential (ETP) (p = 0.031). When the results were analyzed according to the Binet stage, as compared to the controls, the Binet B+C group also presented lower ETP values (p = 0.009). No significant differences were observed between the control and the Binet A groups or between the Binet A and the Binet B + C groups. Although hemostatic alterations may occur in patients with CLL, these parameters do not seem to be useful to indicate disease progression.

https://doi.org/10.1590/s2175-97902022e19407

Evaluation of miR-15a, miR-16-1, ZAP-70, Ang-2, and Bcl-2 as potential prognostic biomarkers in chronic lymphocytic leukemia
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Braga, Tatiane Vieira

; Evangelista, Fernanda Cristina Gontijo

; Santiago, Marie Gabriele

; Ferrão, Aline Lúcia Menezes

; Almeida, Tamara Dauare de

; Barbosa, Bárbara Lima da Fonseca

; Araujo, Sergio Schusterschitz da Silva

; Ribeiro, Glaciano Nogueira

; Carvalho, Maria das Graças

; Sabino, Adriano de Paula

Brazilian Journal of Pharmaceutical Sciences

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2022, Volume 58 elocation e19332

Abstract: En | Text: En | PDF: En

Abstract Chronic lymphocytic leukemia (CLL) is a blood cancer characterized by the accumulation of clonal B-lymphocytes. This study evaluated the mRNA gene expression of miR-15a, miR-16- 1, ZAP-70, and Ang-2 by qPCR, as well as the plasma levels of Bcl-2 by Elisa immunoassay, in CLL patients and healthy controls. Significant differences were observed when comparing patients and controls regarding miR-15a (p < 0.001), miR-16-1 (p < 0.001) mRNA, Ang-2 gene expression, and Bcl-2 plasma levels (p < 0.001). When stratified by risk, differences were maintained with a significantly reduced expression in high-risk patients. A positive correlation was observed between miR-15a and platelets (R2 = 0.340; p = 0.009) as well as between Bcl-2 and leukocytes (R2 = 0.310; p = 0.019). Conversely, negative correlations were observed between ZAP-70 and platelets (R2 = - 0.334; p = 0.011), between miR-15a and lymphocytes (R2 = - 0.376; p = 0.004), as well as between miR-16-and lymphocytes (R2 = - 0.515; p = 0.00004). The data suggest that a reduction in miR-15a and miR-16-1 expressions, in addition to an overexpression of Bcl-2, are associated with the reduction in apoptosis and, consequently, to a longer survival of lymphocytes, thus contributing to lymphocyte accumulation and aggravation of the disease. By contrast, Ang-2 expression was significantly higher in A than in B + C Binet groups. This context leads to the speculation that this biomarker should be investigated in more robust studies within populations with a still relevantly indolent form of the disease in an attempt to identify those patients with a greater potential for an aggravation of the disease.

https://doi.org/10.1590/s2175-97902022e19332

Adherence to humanized care practices for newborns with good vitality in the delivery room
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Schott, Laryssa Cristina

; Góes, Fernanda Garcia Bezerra

; Santos, Andressa Silva Torres dos

; Silva, Aline Cerqueira Santos Santana da

; Pereira-Ávila, Fernanda Maria Vieira

; Goulart, Maithê de Carvalho e Lemos

Revista Gaúcha de Enfermagem

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2022, Volume 43 elocation e20210248

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO Objetivo: Conhecer os fatores intervenientes à adesão dos profissionais de saúde às práticas assistenciais humanizadas ao recém-nascido com boa vitalidade na sala de parto. Método: Pesquisa qualitativa, mediante formulário online com 36 profissionais de saúde atuantes em salas de parto no estado do Rio de Janeiro. Dados processados no Interface de R pour Analyses Multidimensionnelles de Textes Et de Questionnaires e analisados segundo Análise de Conteúdo Temática. Resultados: Identificou-se o contato pele a pele como fator que se relaciona diretamente à realização do clampeamento oportuno do cordão umbilical e amamentação na primeira hora de vida. Outros fatores intervenientes foram: aceitação, formação e categoria profissional; tipo de parto; capacitação e treinamento das equipes. Conclusões: Aprimoramento profissional aliado ao estímulo da chefia, parceria entre pares, boas condições de trabalho, de recursos humanos e de infraestrutura e orientações às famílias propiciam a adesão às práticas assistenciais humanizadas ao recém-nascido na sala de parto.

ABSTRACT Objective: To know the intervening factors in the adherence of health professionals to humanized care practices for newborns with good vitality in the delivery room. Method: Qualitative research, through an online form with 36 health professionals working in delivery rooms in Rio de Janeiro. Data processed in the Interface de R pour Analyses Multidimensionnelles de Textes Et de Questionnaires and analyzed according to Thematic Content Analysis. Results: Skin-to-skin contact was identified as a factor that is directly related to timely clamping of the umbilical cord and breastfeeding in the first hour of life. Other intervening factors were: acceptance, training and professional category; type of delivery; qualification and training of teams. Conclusions: Professional improvement combined with encouragement from the leadership, partnership between peers, good working conditions, human resources and infrastructure and guidance to families provide adherence to humanized care practices for the newborn in the delivery room.

RESUMEN Objetivo: Conocer factores involucrados en la adhesión de los profesionales de la salud a las prácticas de atención humanizada al recién nacido con buena vitalidad en la sala de partos. Método: Investigación cualitativa, utilizando un formulario en línea con 36 profesionales de la salud que trabajan en salas de parto en el estado de Río de Janeiro. Datos procesados mediante la Interface de R pour Analyses Multidimensionnelles de Textes Et de Questionnaires y analizados mediante Thematic Content Analysis. Resultados: El contacto piel con piel se identificó como un factor que está directamente relacionado con la realización del pinzamiento oportuno del cordón umbilical y la lactancia materna en la primera hora de vida. Otros factores fueron: aceptación, formación y categoría profesional; tipo de entrega; calificación y entrenamiento de los equipos. Conclusiones: La superación profesional combinada con el estímulo del liderazgo, la colaboración entre pares, las buenas condiciones de trabajo, los recursos humanos y la infraestructura y la orientación a las familias brindan adherencia a las prácticas de cuidado humanizado del recién nacido en la sala de partos.

https://doi.org/10.1590/1983-1447.2022.20210248.en

Clinical and epidemiological aspects of Delta and Gamma SARS-CoV-2 variant of concern from the western Brazilian Amazon
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Sgorlon, Gabriella

; Queiroz, Jackson Alves da Silva

; Roca, Tárcio Peixoto

; Silva, Ana Maisa Passos da

; Gasparelo, Nadson Willian Felipe

; Teixeira, Karolaine Santos

; Oliveira, Andreia Souza da Nóbrega

; Mendonça, Aline Linhares Ferreira de Melo

; Maia, Adriana Cristina Salvador

; Pereira, Soraya dos Santos

; Batista, Flávia Serrano

; Salcedo, Juan Miguel Villalobos

; Rampazzo, Rita de Cassia Pontello

; Resende, Paola Cristina

; Siqueira, Marilda Mendonça

; Naveca, Felipe Gomes

; Vieira, Deusilene

Memórias do Instituto Oswaldo Cruz

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2022, Volume 117 elocation e220155

Abstract: En | Text: En | PDF: En

BACKGROUND The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants has become a major concern contributing to increased morbidity and mortality worldwide. OBJECTIVES Here we describe the replacement of the Gamma variant of concern (VOC) with Delta in the western Brazilian Amazon. METHODS In this study, we analysed 540 SARS-CoV-2 positive samples determined by qualitative real-time RT-PCR selected in the state of Rondônia between June and December 2021. The positive cohort was sequenced through next-generation sequencing (NGS) and each sample was quantified using real-time RT-qPCR, the whole genome sequence was obtained, SARS-CoV-2 lineages were classified using the system Pango and the maximum likelihood (ML) method was used to conduct phylogenetic analyses. FINDINGS A total of 540 high-quality genomes were obtained, where the Delta VOC showed the highest prevalence making up 72%, with strain AY.43 being the most abundant, while the Gamma VOC was present in 28%, where the P.1 strain was the most frequent. In this study population, only 32.96% (178/540) had completed the vaccination schedule. MAIN CONCLUSIONS This study highlighted the presence of Gamma and Delta variants of SARS-CoV-2 in RO. Furthermore, we observed the replacement of the Gamma VOC with the Delta VOC and its lineages.

https://doi.org/10.1590/0074-02760220155

Cannabinoids in Neurology - Position paper from Scientific Departments from Brazilian Academy of Neurology
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BRUCKI, Sonia Maria Dozzi

; ADONI, Tarso

; ALMEIDA, Carlos Mauricio Oliveira

; ANDRADE, Daniel Ciampi de

; ANGHINAH, Renato

; BARBOSA, Luciana Mendonça

; BAZAN, Rodrigo

; CARVALHO, Alzira Alves de Siqueira

; CARVALHO, William

; CHRISTO, Paulo Pereira

; COLETTA, Marcus Della

; CONFORTO, Adriana Bastos

; CORREA-NETO, Ylmar

; ENGELHARDT, Eliasz

; FRANÇA JUNIOR, Marcondes Cavalcante

; FRANCO, Clelia

; VON GLEHN, Felipe

; GOMES, Helio Rodrigues

; HOULY, Caroline Gomes de Barros

; KAUP, Alexandre Ottoni

; KOWACS, Fernando

; KANASHIRO, Aline

; LOPES, Victor Gonçalves

; MAIA, Débora

; MANREZA, Maria

; MARTINEZ, Alberto Rolim Muro

; MARTINEZ, Sandra Cristina Gonçalves

; NADER, Saulo Nardy

; NEVES, Luciana de Oliveira

; OKAMOTO, Ivan Hideyo

; OLIVEIRA, Rogério Adas Ayres de

; PEIXOTO, Fabiano de Melo

; PEREIRA, Cristiana Borges

; SABA, Roberta Arb

; SAMPAIO, Leticia Pereira de Brito

; SCHILLING, Lucas Porcello

; SILVA, Marcus Tulius Teixeira

; SILVA, Emanuelle Roberta

; SMID, Jerusa

; SOARES, Cristiane Nascimento

; SOBREIRA-NETO, Manoel

; SOUSA, Nise Alessandra de Carvalho

; SOUZA, Leonardo Cruz de

; TEIVE, Hélio Afonso Ghizoni

; TERRA, Vera Cristina

; VALE, Matheus

; VIEIRA, Vitor Mendes Grise

; ZANOTELI, Edmar

; PRADO, Gilmar

Arquivos de Neuro-Psiquiatria

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apr 2021, Volume 79 N. 4 Pages 354 - 369

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Os canabinoides compreendem os endocanabinoides, fitocanabinoides e os canabinoides sintéticos e desempenham ações no sistema nervoso central e periférico. Uma quantidade enorme de publicações tem sido lançada nos últimos anos, embora a cannabis seja conhecida por milênios. Os Departamentos Científicos da Academia Brasileira de Neurologia descreveram as evidências do uso médico em suas áreas. A literatura está em constantes mudanças e possíveis novas evidências podem surgir nos próximos dias ou meses. A prescrição dessas substâncias deve ser discutida com os pacientes e suas famílias, com conhecimento sobre eventos adversos e sua eficácia.

ABSTRACT Cannabinoids comprehend endocannabinoids, phytocannabinoids, and synthetic cannabinoids, with actions both in the central and peripherical nervous systems. A considerable amount of publications have been made in recent years, although cannabis has been known for over a thousand years. Scientific Departments from the Brazilian Academy of Neurology described evidence for medical use in their areas. Literature is constantly changing, and possible new evidence can emerge in the next days or months. Prescription of these substances must be discussed with patients and their families, with knowledge about adverse events and their efficacy.

https://doi.org/10.1590/0004-282x-anp-2020-0432 1049 downloads

Morphology and morphogenesis of SARS-CoV-2 in Vero-E6 cells
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Barreto-Vieira, Debora Ferreira

; da Silva, Marcos Alexandre Nunes

; Garcia, Cristiana Couto

; Miranda, Milene Dias

; Matos, Aline da Rocha

; Caetano, Braulia Costa

; Resende, Paola Cristina

; Motta, Fernando Couto

; Siqueira, Marilda Mendonça

; Girard-Dias, Wendell

; Archanjo, Bráulio Soares

; Barth, Ortrud Monika

Memórias do Instituto Oswaldo Cruz

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2021, Volume 116 elocation e200443

Abstract: En | Text: En | PDF: En

BACKGROUND The coronaviruses (CoVs) called the attention of the world for causing outbreaks of severe acute respiratory syndrome (SARS-CoV), in Asia in 2002-03, and respiratory disease in the Middle East (MERS-CoV), in 2012. In December 2019, yet again a new coronavirus (SARS-CoV-2) first identified in Wuhan, China, was associated with a severe respiratory infection, known today as COVID-19. This new virus quickly spread throughout China and 30 additional countries. As result, the World Health Organization (WHO) elevated the status of the COVID-19 outbreak from emergency of international concern to pandemic on March 11, 2020. The impact of COVID-19 on public health and economy fueled a worldwide race to approve therapeutic and prophylactic agents, but so far, there are no specific antiviral drugs or vaccines available. In current scenario, the development of in vitro systems for viral mass production and for testing antiviral and vaccine candidates proves to be an urgent matter. OBJECTIVE The objective of this paper is study the biology of SARS-CoV-2 in Vero-E6 cells at the ultrastructural level. METHODS In this study, we documented, by transmission electron microscopy and real-time reverse transcription polymerase chain reaction (RT-PCR), the infection of Vero-E6 cells with SARS-CoV-2 samples isolated from Brazilian patients. FINDINGS The infected cells presented cytopathic effects and SARS-CoV-2 particles were observed attached to the cell surface and inside cytoplasmic vesicles. The entry of the virus into cells occurred through the endocytic pathway or by fusion of the viral envelope with the cell membrane. Assembled nucleocapsids were verified inside rough endoplasmic reticulum cisterns (RER). Viral maturation seemed to occur by budding of viral particles from the RER into smooth membrane vesicles. MAIN CONCLUSIONS Therefore, the susceptibility of Vero-E6 cells to SARS-CoV-2 infection and the viral pathway inside the cells were demonstrated by ultrastructural analysis.

https://doi.org/10.1590/0074-02760200443 1922 downloads

Adverse events following immunization in pregnant women from Minas Gerais
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Silveira, Isabela Oliveira da

; Silva, Thales Philipe Rodrigues da

; Luvisaro, Bianca Maria Oliveira

; Silva, Roberta Barros da

; Gusmão, Josianne Dias

; Vimieiro, Aline Mendes

; Oliveira, Valéria Conceição de

; Souza, Karina Cristina Rouwe de

; Faria, Ana Paula Vieira

; Matozinhos, Fernanda Penido

Revista de Saúde Pública

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2021, Volume 55 elocation 24

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO OBJETIVO: Analisar a distribuição dos eventos adversos pós-vacinação (EAPV) em gestantes do estado de Minas Gerais, entre 2015 e 2019. MÉTODOS: Estudo epidemiológico, descritivo, realizado com dados de EAPV, do período de 2015 a 2019, registrados no Sistema de Informação da Vigilância de Eventos Adversos, no estado de Minas Gerais (MG), Brasil. Foram analisados 670 EAPV em gestantes. As estimativas foram apresentadas em proporções, de acordo com o ano de ocorrência, macrorregião de saúde de Minas Gerais e imunobiológico administrado. RESULTADOS: O ano em que mais houve registros foi 2017 (36,8%). Entre as 14 macrorregiões, as com menor e maior número de registros foram o Vale do Jequitinhonha (0,5%) e o Centro (31,8%), respectivamente. As vacinas contraindicadas durante a gestação representaram 27,6% do total de notificações. Foram considerados como erros de imunização 69,5% dos casos. Em 75,9% dos registros, a variável de atendimento médico foi ignorada, e em 73,7% dos casos não foi apresentada informação sobre a evolução. CONCLUSÃO: Este estudo expõe a necessidade da educação continuada para a equipe multidisciplinar, a fim de reduzir casos de EAPV e garantir o preenchimento adequado das notificações pelos profissionais de saúde.

ABSTRACT OBJECTIVE: To analyze the distribution of adverse events following immunization (AEFI) in pregnant women in the state of Minas Gerais, between 2015 and 2019. METHODS: This is an epidemiological, descriptive study conducted with AEFI data from 2015 to 2019, recorded in the Adverse Events Surveillance Information System, in the state of Minas Gerais (MG), Brazil. A total of 670 AEFI were analyzed in pregnant women. The estimates were presented in proportions, according to the year of occurrence, health macro-region of Minas Gerais and immunobiological administered. RESULTS: The year in which there were the most records was 2017 (36.8%). Among the 14 macro-regions, the ones with the lowest and highest number of records were the Vale do Jequitinhonha (0.5%) and Center (31.8%), respectively. The vaccines contraindicated during pregnancy represented 27.6% of the total notifications. The total of 69.5% of the cases were considered immunization errors. In 75.9% of the records, the variable of medical care was ignored, and in 73.7% of the cases no information on the evolution was presented. CONCLUSION: This study shows the need for continuing education for the multidisciplinary team, in order to reduce cases of AEFI and ensure the adequate completion of notifications by health professionals.

https://doi.org/10.11606/s1518-8787.2021055002592

Adverse events following immunization in pregnant women from Minas Gerais
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Silveira, Isabela Oliveira da

; Silva, Thales Philipe Rodrigues da

; Luvisaro, Bianca Maria Oliveira

; Silva, Roberta Barros da

; Gusmão, Josianne Dias

; Vimieiro, Aline Mendes

; Oliveira, Valéria Conceição de

; Souza, Karina Cristina Rouwe de

; Faria, Ana Paula Vieira

; Matozinhos, Fernanda Penido

Revista de Saúde Pública

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2021, Volume 55 elocation 24

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.11606/s1518-8787.2021055002592 339 downloads

10.

Whole chickpea flour as an ingredient for improving the nutritional quality of sandwich bread: Effects on sensory acceptance, texture profile, and technological properties
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Costa, Regina Teixeira da

; Silva, Sinara Cristina da

; Silva, Luana Sousa

; Silva, Washington Azevêdo da

; Gonçalves, Aline Cristina Arruda

; Pires, Christiano Vieira

; Martins, Amanda Mattos Dias

; Chávez, Davy William Hidalgo

; Trombete, Felipe Machado

Revista chilena de nutrición

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dec 2020, Volume 47 N. 6 Pages 933 - 940

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

ABSTRACT The inclusion of pulse flours with higher dietary fiber content in bakery products, such as chickpea (Cicer arietinum L) flour, has been exploited by the food industry due to its nutritional and sensory characteristics. This work aimed to develop a sandwich bread with a partial substitution (7.5%, 15%, and 30%) of refined wheat flour by whole chickpea flour (WCF) and also to evaluate the effects on sensory acceptance, physicochemical parameters, and texture profile during the shelf life. Four methods for obtaining WCF were assessed and the best condition was used to produce the sandwich bread. Sensory evaluation was carried out with 65 consumers using a 9-point hedonic scale and a purchase intention scale. Firmness, elasticity, cohesiveness, chewability, and fracturability was evaluated by the Texture Profile Analysis (TPA). The specific volume, total fungi count, pH, titratable acidity, and moisture content were also determined. The nutritional composition was estimated by mass balance. Data demonstrated that there was no difference (p>0.05) in sensory evaluation of the products elaborated with different concentrations of WCF, and all samples presented good acceptance. The addition of WCF did not cause negative effects on the texture and volume characteristics of the bread. The sample with 30% WCF had adequate technological characteristics, resulting in a product with higher nutritional value, especially dietary fiber, and was well accepted by the consumers. This formulation can be used by the food industry and also in domestic preparations, contributing to the diversification and nutritional enrichment of sandwich bread.

RESUMEN La inclusión de harinas de granos no convencionales con mayor contenido de fibra dietética en productos de panadería, como la harina integral de garbanzo (HIG) (Cicer arietinum L), es utilizada por la industria alimentaria debido a sus características nutricionales y sensoriales. Este trabajo tuvo como objetivo desarrollar un pan para sándwich (PPS) con sustitución parcial (7.5%, 15% y 30%) de harina de trigo refinada por HIG así como evaluar la aceptación sensorial, parámetros fisicoquímicos y el perfil de textura durante su vida útil. Fueron evaluados cuatro métodos para la obtención de HIG, utilizándose la mejor condición para producir el PPS. Sesenta y cinco consumidores realizaron la evaluación de aceptación sensorial e intención de compra con una escala hedónica de 9 puntos. La firmeza, la elasticidad, la cohesión, la masticabilidad y la fracturabilidad se evaluaron mediante el análisis de perfil de textura (TPA). También se determinó el volumen específico, el recuento total de hongos, el pH, la acidez titulable y el contenido de humedad. La composición nutricional se estimó por balance de masa. Todas las muestras presentaron buena aceptabilidad sin diferencias estadística. La adición de HIG no causó efectos negativos en la textura ni el volumen del PPS. La muestra con 30% de HIG tubo características tecnológicas adecuadas, resultando en un producto con mayor valor nutricional, especialmente por la fibra dietética. Esta formulación podría ser utilizada por la industria alimentaria y también en preparaciones domésticas, contribuyendo a la diversificación y al enriquecimiento nutricional del pan para sándwich.

595 downloads

11.

Clinical and molecular correlates of the ASPECTS in the acute phase of stroke
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MOURÃO, Aline Mansueto

; VICENTE, Laélia Cristina Caseiro

; ABREU, Mery Natali Silva

; SANT’ANNA, Romeu Vale

; DE MEIRA, Fidel Castro Alves

; XAVIER, Rodrigo Menezes de Brito

; TANURE, Marco Túlio de Azevedo

; VIEIRA, Erica Leandro Marciano

; DE SOUZA, Leonardo Cruz

; MIRANDA, Aline Silva de

; RACHID, Milene Alvarenga

; TEIXEIRA, Antônio Lucio

Arquivos de Neuro-Psiquiatria

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may 2020, Volume 78 N. 5 Pages 262 - 268

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Introdução: A Alberta Stroke Early Score (ASPECTS) foi desenvolvida para monitorização de alterações isquêmicas precoces na tomografia computadorizada de crânio, estando associada a desfechos clínicos. A ASPECTS também pode se associar aos biomarcadores periféricos que refletem a resposta fisiopatológica do cérebro ao AVC isquêmico. Objetivo: Investigar à associação entre os parâmetros periféricos com a Alberta Stroke Early Score (ASPECTS) em indivíduos após acidente vascular cerebral isquêmico. Métodos: Pacientes acima de 18 anos com AVC isquêmico agudo foram incluídos neste estudo. Nenhum paciente foi elegível para trombólise. Os pacientes foram submetidos à tomografia computadorizada sem contraste nas primeiras 24 horas da admissão, a ASPECTS e as avaliações clínicas e moleculares aplicadas no mesmo dia. O National Institutes of Health Stroke Scale (NIHSS), a escala de Rankin modificada e o Mini Exame do Estado Mental para avaliação clínica também foram aplicados a todos os indivíduos. Os níveis plasmáticos de BDNF, VCAM-1, VEGF, IL-1β, sTNFRs e adiponectina foram determinados por ELISA. Resultados: Pior desempenho neurológico (NIHSS), cognitivo (MEEM) e funcional (Rankin) foram observados no grupo com alterações na ASPECTS. Pacientes com alterações na ASPECTS também exibiram níveis mais altos de IL-1β e adiponectina. Na regressão multivariada linear, foi encontrado um coeficiente R ajustado de 0,515, indicando adiponectina e NIHSS como preditores independentes para a ASPECTS. Conclusão: Os níveis plasmáticos de adiponectina estão associados aos escores da ASPECTS.

ABSTRACT Background: The Alberta Stroke Program Early CT Score (ASPECTS) scale was developed for monitoring early ischemic changes on CT, being associated with clinical outcomes. The ASPECTS can also associate with peripheral biomarkers that reflect the pathophysiological response of the brain to the ischemic stroke. Objective: To investigate the association between peripheral biomarkers with the Alberta Stroke Program Early CT Score (ASPECTS) in individuals after ischemic stroke. Methods: Patients over 18 years old with acute ischemic stroke were enrolled in this study. No patient was eligible for thrombolysis. The patients were submitted to non-contrast CT in the first 24 hours of admission, being the Alberta Stroke Program Early CT Score and clinical and molecular evaluations applied on the same day. The National Institutes of Health Stroke Scale (NIHSS), modified Rankin scale and the Mini-Mental State Examination for clinical evaluation were also applied to all subjects. Plasma levels of BDNF, VCAM-1, VEGF, IL-1β, sTNFRs and adiponectin were determined by ELISA. Results: Worse neurological impairment (NIHSS), cognitive (MEEM) and functional (Rankin) performance was observed in the group with changes in the NCTT. Patients with NCTT changes also exhibited higher levels of IL-1β and adiponectin. In the linear multivariate regression, an adjusted R coefficient of 0.515 was found, indicating adiponectin and NIHSS as independent predictors of ASPECTS. Conclusion: Plasma levels of adiponectin are associated with the ASPECTS scores.

https://doi.org/10.1590/0004-282x20200001 1343 downloads

12.

Water sustainability of the Guamá river basin, Eastern Amazonia/Brazil
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Rocha, Nívia Cristina Vieira

; Lima, Aline Maria Meiguins de

Sociedade & Natureza

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2020, Volume 32 Pages 130 - 148

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo A bacia hidrográfica do rio Guamá abrange 19 municípios localizados no nordeste do Pará, com grande diversidade de formas de uso da terra e a maior densidade demográfica do estado. Nesta pesquisa foi avaliada sua sustentabilidade hídrica, a partir dos aspectos hidrológicos, ambientais, sociais e de gestão, nas suas 8 sub-bacias componentes. O indicador hidrológico demonstrou um comportamento mediano para às sub-bacias; o ambiental destacou a fragilidade da cobertura vegetal resiliente; o social apresentou o pior desempenho; e o de gestão indicou a necessidade do fortalecimento institucional. A bacia do rio Guamá no geral obteve um índice de sustentabilidade intermediário, porém os resultados parciais apontam para necessidade de medidas voltadas ao planejamento estratégico e ao manejo, para minimizar as pressões sobre a vegetação remanescente, reforçar a capacidade institucional e melhorar a qualidade dos recursos e de vida da população, com a intenção de potencializar a sustentabilidade da bacia.

Abstract The Guamá River basin covers 19 municipalities located in northeastern Pará, with great diversity of land use forms and the highest population density of the state. In this research, its water sustainability was evaluated, based on the hydrological, environmental, social and management aspects, in its 8 sub-basins. The hydrological indicator showed a medium behavior for the sub-basins; the environmental highlighted the fragility of the resilient vegetal cover; the social performance presented the worst performance; and the management pointed out the need for institutional strengthening. The Guamá river basin in general obtained an intermediate sustainability index, but the partial results indicated that for measures geared to the strategic planning and management, to minimize pressures on the remaining vegetation, enhancing institutional capacity and improving the quality of life of the population and resources, with the intention of enhancing the sustainability of the basin.

https://doi.org/10.14393/sn-v32-2020-45694

13.

PRESCRIÇÃO E USO DE METRONIDAZOL PARA CONTROLE DO ODOR EM FERIDAS NEOPLÁSICAS
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Souza, Nauã Rodrigues de

; Lima, Maria Theresa Camilo de

; Batista, Rayanne Paullyne da Silva

; Santos, Aline Milany da Silva

; Bushatsky, Magaly

; Santos, Isabel Cristina Ramos Vieira

Cogitare Enfermagem

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2019, Volume 24 elocation e57906

Abstract: En Es Pt | Text: En Es Pt | PDF: En | PDF: Es | PDF: Pt

ABSTRACT Objective To check aspects related to the prescription, preparation and administration of metronidazole for the control of odor in neoplastic wounds. Methodology Cross-sectional study with 80 health professionals from five reference hospitals in oncology in Recife-PE, between August and October 2017. The following variables were analyzed: professional characterization, criteria for prescription, presentation, dilution, application, frequency and care in the application. Mean and standard deviation were calculated for obtaining the discrete rational variables, and chi-square test with Yates correction was used for dichotomous categorical variables. Results The nurses had little professional experience (1-3 years), less specialization compared to physicians, but had greater expertise in palliative care. Regarding the prescription and use of the product, 14 (53.8%) professionals used alternative and empirical prescriptions, with maceration of tablets, and five (19.3%) used injectable solution. Conclusion According to the results obtained, there are few studies on the subject and protocols based on scientific evidence should be constructed.

RESUMEN Objetivo verificar los aspectos asociados a la prescripción, preparación y administración del metronidazol para control del olor en heridas neoplásicas. Metodología estudio transversal, con 80 profesionales de salud de cinco hospitales referencia en oncología en Recife, PE, entre agosto y octubre de 2017. Se analizaron las variables: caracterización profesional, criterios para prescripción, presentación, dilución, aplicación, frecuencia y cuidados en la aplicación. Se calcularon el promedio y la desviación típica para las variables racionales discretas, y para categóricas dicotómicas, test chi cuadrado con corrección de Yates. Resultados los enfermeros se caracterizaron por el poco tiempo de experiencia (1-3 años), menor frecuencia de especialización en comparación con los médicos, pero con más frecuencia de actualización en cuidados paliativos. Acerca de la prescripción y uso del producto, se observaron prescripciones alternativas y empíricas, con maceración de pastillas 14 (53,8%) o solución inyectable en cinco (19,3%). Conclusión resultados evidencian la escasa literatura sobre la temática y apuntan a la necesidad de construcción de protocolos basados en evidencias científicas.

RESUMO Objetivo verificar os aspectos relacionados à prescrição, preparo e administração do metronidazol para controle do odor em feridas neoplásicas. Metodologia estudo transversal, com 80 profissionais de saúde de cinco hospitais referência em oncologia em Recife-PE, entre agosto e outubro de 2017. Foram analisadas as variáveis: caracterização profissional, critérios para prescrição, apresentação, diluição, aplicação, frequência e cuidados na aplicação. Calculou-se média e desvio padrão para as variáveis racionais discretas, e para categóricas dicotômicas, teste qui-quadrado com correção de Yates. Resultados os enfermeiros caracterizaram-se pelo pouco tempo de experiência (1-3 anos), menor frequência de especialização comparados aos médicos, porém maior frequência de atualização em cuidados paliativos. Quanto à prescrição e utilização do produto, observou-se prescrições alternativas e empíricas, com maceração de comprimidos 14 (53,8%) ou solução injetável em cinco (19,3%). Conclusão resultados evidenciam a escassa literatura sobre a temática e levantam a necessidade de construção de protocolos fundamentados em evidências científicas.

https://doi.org/10.5380/ce.v24i0.57906 427 downloads

14.

T-lymphocyte-expressing inflammatory cytokines underlie persistence of proteinuria in children with idiopathic nephrotic syndrome
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Guimarães, Fábio Tadeu Lourenço

; Melo, Gustavo Eustáquio Brito Alvim de

; Cordeiro, Thiago Macedo

; Feracin, Victor

; Vieira, Etel Rocha

; Pereira, Wagner de Fátima

; Pinheiro, Sérgio Veloso Brant

; Miranda, Aline Silva

; Simões-e-Silva, Ana Cristina

Jornal de Pediatria

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oct 2018, Volume 94 N. 5 Pages 546 - 553

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Objetivo Há comprovação do importante papel das alterações no sistema imunológico no desencadeamento e manutenção da síndrome nefrótica idiopática (SNI). O objetivo deste estudo foi investigar a expressão das citocinas em populações de linfócitos de pacientes com SNI em comparação a indivíduos saudáveis e de acordo com a proteinúria. Métodos Este estudo transversal incluiu 44 pacientes com SNI e oito crianças saudáveis, pareados por idade e sexo (controles). Os pacientes foram subdivididos de acordo com a proteinúria: proteinúria persistente ou remissão parcial (PP ≥ 300 mg/24 h, n = 17) e proteinúria baixa ou remissão completa (PB < 300 mg/24 h, n = 27). A análise ex vivo de leucócitos no sangue periférico por citometria de fluxo foi feita utilizando marcadores de superfície para linfócitos T, TCD4, TCD8, células natural killer (NK), linfócitos NKT e B. As frequências das citocinas intracelulares foram analisadas nessas células. Resultados A frequência dos linfócitos B, células NK e células NKT foi menor em pacientes com SNI do que nos controles, ao passo que os pacientes com SNI apresentaram maior frequência de células CD4+fator de necrose tumoral (TNF)-α+ do que nos controles. Os linfócitos T citotóxicos que expressam interferon (IFN)-γ foram menores nos pacientes com SNI do que nos controles. Os pacientes com PP mostraram maiores frequências de linfócitos T CD4 que expressam IFN-γ e TNF-α que os controles. Os linfócitos CD8 que expressam TNF-α apresentaram aumento no grupo com PP, em comparação aos com PB e os controles, apesar de as células CD8+IFN-γ+ serem mais baixas nos pacientes com PB e nos controles. Conclusão Com relação ao nível de proteinúria, os pacientes com SNI apresentaram aumento na expressão de TNF-α nos linfócitos CD4 e expressão reduzida de IFN-γ nos linfócitos CD8. A persistência da proteinúria foi associada a maiores níveis de marcadores inflamatórios.

Abstract Objective: There is evidence of an important role of immune system changes in the triggering and maintenance of idiopathic nephrotic syndrome (INS). The aim of this study was to investigate the expression of cytokines in lymphocyte populations of patients with INS in comparison to healthy individuals, according to proteinuria. Methods: This cross-sectional study included 44 patients with INS and eight healthy children, matched for age and sex (controls). Patients were subdivided according to proteinuria: persistent proteinuria or partial remission (PP ≥ 300 mg/24 h, n = 17) and low proteinuria or complete remission (LP < 300 mg/24 h, n = 27). Ex vivo analysis of peripheral blood leukocytes by flow cytometry was performed using surface markers for T-lymphocytes, TCD4, TCD8, natural killer (NK) cells, NKT, and B-lymphocytes. Frequencies of intracellular cytokines were analyzed in these cells. Results: The frequencies of B-lymphocytes, NK cells, and NKT cells were lower in INS than in controls, whereas INS patients had a higher frequency of CD4+tumor necrosis factor (TNF)-α+ cells than controls. Cytotoxic-T-lymphocytes expressing IFN-γ were lower in INS than in controls. Patients with PP showed higher frequencies of CD4-T-lymphocytes expressing IFN-γ and TNF-α than controls. CD8-lymphocytes expressing TNF-α were increased in PP group when compared with LP and controls, while CD8+interferon (IFN)-γ+ cells were lower than in LP and in controls. Conclusion: Regardless the level of proteinuria, INS patients had increased expression of TNF-α in CD4-lymphocytes and reduced expression of IFN-γ in CD8-lymphocytes. Persistence of proteinuria was associated with higher levels of inflammatory markers.

https://doi.org/10.1016/j.jped.2017.08.005 2253 downloads

15.

Sistema universal de saúde e cobertura universal: desvendando pressupostos e estratégias
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Giovanella, Ligia

; Mendoza-Ruiz, Adriana

; Pilar, Aline de Carvalho Amand

; Rosa, Matheus Cantanhêde da

; Martins, Gabrieli Branco

; Santos, Isabela Soares

; Silva, Danielle Barata

; Vieira, Jean Mendes de Lucena

; Castro, Valeria Cristina Gomes de

; Silva, Priscilla Oliveira da

; Machado, Cristiani Vieira

Ciência & Saúde Coletiva

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jun 2018, Volume 23 N. 6 Pages 1763 - 1776

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Nos últimos anos, acirrou-se o debate internacional sobre diferentes concepções de universalidade em saúde, polarizado nas propostas de sistema universal versus cobertura universal em saúde. A concepção de cobertura universal tem sido difundida por organizações internacionais e incorporada às reformas dos sistemas de saúde de alguns países em desenvolvimento, inclusive na América Latina. O artigo explora os pressupostos e as estratégias relacionados à proposta de cobertura universal de saúde. Inicialmente contrastam-se as concepções de universalidade nos modelos de cobertura universal e de sistemas universais de saúde. A seguir, contextualiza-se o debate internacional, incluindo exemplos de diferentes sistemas de saúde. Por fim, discutem-se as implicações da concepção de cobertura universal para o direito à saúde no Brasil. A análise das diferentes concepções de universalidade e das experiências de países evidenciam que arranjos de seguros (privados ou sociais) não superam as fortalezas de sistemas públicos universais de saúde. A compreensão dos projetos em disputa no cenário internacional é fundamental para identificar possibilidades e ameaças à consolidação do Sistema Único de Saúde no Brasil.

Abstract In recent years the international debate about universality in health has been marked by a polarization between ideas based on a universal system, and notions proposing universal health coverage. The concept of universal coverage has been disseminated by international organizations and has been incorporated into health system reforms in several developing countries, including some in Latin America. This article explores the assumptions and strategies related to the proposal of universal health coverage. Firstly, a comparison is provided of the models of universal health coverage and universal health systems. This is followed by a contextualization of the international debate, including examples of different health systems. Finally, the implications of the proposal of universal coverage for the right to health in Brazil are discussed. The analysis of different concepts of universality and the experiences of different countries shows that health insurance-based models, either social or private, are not as satisfactory as public, universal health systems. Greater understanding about ongoing international projects is essential in order to identify the possibilities represented by the consolidation of the Unified Health System (SUS) in Brazil, as well as the risks of dismantling the SUS.

https://doi.org/10.1590/1413-81232018236.05562018 57 downloads

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