Abstract Objective To develop a health education mode based on the theory of planned behavior (TPB), and to explore the influence of this mode on the postoperative lifestyle of patients with cervical cancer. Methods A total of 122 patients diagnosed from January 2019 to June 2020 were divided into control and education groups (n=61) using a random number table. Control group received traditional health education after operation, while education group was given TPB-based health education after operation. Their general data, medication compliance, dietary compliance, discharge time, incidence of complications, lifestyle, quality of life (QOL) and mental state before and after operation were compared. Results No significant differences were found in general data, preoperative lifestyle score, QOL score and mental state score between the two groups (P>0.05). In education group, the medication compliance and dietary compliance scores were significantly higher than those in control group, while the discharge time and the total incidence of complications were earlier and lower than those in control group (P<0.05). After operation, the scores of self-realization, interpersonal relationship, nutrition, physical activity, health responsibility, stress management, physical dimension, social/family dimension, emotional dimension and functional dimension significantly rose, while the distress thermometer and problem list scores declined in both groups, and education group had better improvement in scores than control group (P<0.05). Conclusion Theory of planned behavior health education mode can improve the postoperative treatment compliance, reduce postoperative complications, and ameliorate the lifestyle, quality of life and mental state of patients with cervical cancer.

Resumen Objetivo Elaborar una modalidad de educación para la salud basada en la teoría del comportamiento planeado (TCP) e investigar su influencia en el estilo de vida posoperatorio de pacientes con cáncer cervical. Métodos Un total de 122 pacientes diagnosticados de enero de 2019 a junio de 2020 fueron divididos entre los grupos Control y Educación (n=61) por medio de una planilla de número aleatorios. El grupo Control recibió educación para la salud tradicional luego de la cirugía, mientras que el grupo Educación recibió educación para la salud basada en la TCP después de la cirugía; se compararon los datos generales, la adherencia a la medicación, la adherencia a la dieta, el tiempo de alta, la incidencia de complicaciones, el estilo de vida, la calidad de vida (CV) y el estado mental antes y después de la cirugía. Resultados No se encontraron diferencias significativas entre los dos grupos en datos generales, puntaje de estilo de vida preoperatorio, puntaje de calidad de vida y puntaje de estado mental (P>0,05). En el grupo Educación, los puntajes de adherencia a la medicación y adherencia a la dieta fueron significativamente más altos que en el grupo Control; mientras que el tiempo de alta fue anterior y la incidencia total de complicaciones fue menor que en el grupo Control (P <0,05). Después de la cirugía, los puntajes de autorrealización, relaciones interpersonales, nutrición, actividad física, responsabilidad por la salud, gestión del estrés, dimensión física, dimensión social/familiar, dimensión emocional y dimensión funcional aumentaron significativamente; mientras que los puntajes del termómetro de sufrimiento y de la lista de problemas disminuyeron en ambos grupos y la mejora de los puntajes fue mayor en el grupo Educación que en el grupo Control (P <0,05). Conclusión La modalidad de educación para la salud basada en la TCP puede mejorar la adherencia al tratamiento posoperatorio, reducir las complicaciones posoperatorias y mejorar el estilo de vida, la calidad de vida y el estado mental de paciente con cáncer cervical.

Resumo Objetivo Desenvolver uma modalidade de educação em saúde baseado na teoria do comportamento planejado (TCP) e explorar sua influência no estilo de vida pós-operatório de pacientes com câncer cervical. Métodos Um total de 122 pacientes diagnosticados de janeiro de 2019 a junho de 2020 foram divididos nos grupos Controle e Educação (n = 61) usando uma tabela de números aleatórios. O grupo Controle recebeu educação em saúde tradicional após a cirurgia, enquanto o grupo Educação recebeu educação em saúde baseada em TCP após a cirurgia e seus dados gerais, adesão à medicação, adesão à dieta, tempo de alta, incidência de complicações, estilo de vida, qualidade de vida (QV) e estado mental antes e depois da cirurgia foram comparados. Resultados Não foram encontradas diferenças significativas nos dados gerais, pontuação de estilo de vida pré-operatório, pontuação de qualidade de vida e pontuação de estado mental entre os dois grupos (P>0,05). No grupo Educação, as pontuações de adesão à medicação e adesão à dieta foram significativamente maiores do que no grupo Controle, enquanto o tempo de alta e a incidência total de complicações foram mais precoces e menores do que no grupo Controle (P <0,05). Após a cirurgia, as pontuações de autorrealização, relacionamento interpessoal, nutrição, atividade física, responsabilidade pela saúde, gestão do estresse, dimensão física, dimensão social/familiar, dimensão emocional e dimensão funcional aumentaram significativamente, enquanto as pontuações do termômetro de sofrimento e da lista de problemas diminuíram em ambos os grupos, e a melhora nas pontuações foi maior no grupo Educação do que o grupo Controle (P <0,05). Conclusão A modalidade de educação em saúde baseada na TCP pode melhorar a adesão ao tratamento pós-operatório, reduzir as complicações pós-operatórias e melhorar o estilo de vida, a qualidade de vida e o estado mental de pacientes com câncer cervical.

Abstract Objective Nontuberculous mycobacteria (NTM) species are increasingly being isolated and have become a key factor affecting public health by causing pulmonary diseases. Most NTM species do not respond to conventional tuberculosis (TB) drugs. This study aimed to identify NTM isolated from suspected pulmonary TB patients from the Zhejiang province and analyze their distribution in the region. Methods A total of 1,113 NTM isolates from patients suspected to be suffering from acid-fast bacilli-positive tuberculosis were identified at the species level, using the CapitalBio Mycobacterium identification array and polymerase chain reaction amplification and sequencing of 16S-23S gene internal transcribed spacer (ITS), 16S rRNA, and hsp65. Results Of the 23,138 isolates, we identified 1,102 NTM (4.8%), mainly including Mycobacterium intracellulare (54.81%, 604/1,102), M. chelonae-M. abscessus (16.52%, 182/1,102), M. avium (13.16%, 145/1,102), M. kansasii (8.17%, 90/1,102), and M. gordonae (3.27%, 36/1,102). Conclusion The distribution of NTM species observed in patients with suspected pulmonary tuberculosis provides guidance for the diagnosis and treatment of NTM pulmonary diseases.

Abstract Purpose: To evaluate the effects of 1,25 dihydroxy vitamin D3 (1,25(OH)2D3) on the content of triglyceride (TG), as well as on the gene and protein expressions of adiponectin receptor 2 (AdipoR2), p38 mitogen-activated protein kinase (P38MAPK), and lipoprotein lipase (LPL) in the liver of rats with type 2 diabetes mellitus (T2DM) so as to provide theoretical basis for exploring the mechanism by which 1,25(OH)2D3 regulates TG. Methods: Wistar rats were divided into four groups (n=25), with different treatments and detected the gene and protein expressions of AdipoR2, p38MAPK, and LPL in the liver tissue by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. Meanwhile, the content of TG in the liver tissue was detected by the Enzyme-linked immunosorbent assay. Results: The expression of AdipoR2, p38MAPK, LPL gene and protein in the liver of VitD intervention group was significantly higher than that in T2DM group (P <0.05), while the TG content was significantly lower than that in T2DM group (P <0.05). Conclusion: 1,25(OH)2D3 can decrease the content of TG in the liver, and its mechanism may be achieved by upregulating the expressions of AdipoR2, p38MAPK, and LPL in the liver.

OBJECTIVE: This study investigated serum interleukin-10 (IL-10) levels, changes in peripheral blood CD4+CD25+ regulatory T cell (PBCDT) ratios, and the prognosis of cervical cancer (CC) patients. METHODS: Seventy patients with CC composed the observation group, and 70 healthy subjects composed the control group. The PBCDT ratios in the CC patients and healthy subjects were calculated. Serum IL-10 levels were detected with a double antibody sandwich enzyme-linked immunosorbent assay (ELISA). RESULTS: The PBCDT ratio was higher in the patients with active CC [12.16±2.41%] than in the control subjects [6.34±1.05%]. Serum IL-10 levels were higher in the patients with CC [384±106 pg/ml] than in the control subjects [104±50 pg/ml]; the differences in both PBCDT ratio and IL-10 level were statistically significant (p<0.01). Serum IL-10 levels were positively correlated with PBCDT ratios (r=0.375, p<0.05). The 5-year patient survival rate was significantly higher in the low serum IL-10 group (64.2%) than in the high serum IL-10 group (42.8%, p=0.012). CONCLUSIONS: PBCDT ratios and serum IL-10 levels are related to CC activity. These factors are reciprocally related and influence one another, and both are involved in the development and progression of CC. Low IL-10 expression is beneficial regarding the survival of patients with CC.

The aim of this meta-analysis was to compare the efficacy of metronidazole and vancomycin for the treatment of Clostridium difficileinfection, especially to investigate which agent was superior for treating either mild or severe C. difficileinfection. A meta-analysis of randomized controlled trials and cohort studies identified in Pubmed, Embase, and the Cochrane Library was conducted. Four randomized controlled trials and two cohort studies involving 1218 patients were included in this meta-analysis. Metronidazole was inferior to vancomycin for treating C. difficileinfection in terms of both initial clinical cure rates (risk ratio, RR = 0.91, 95% confidence interval, CI = 0.84-0.98, p= 0.02) and sustained cure rates (RR = 0.88, 95% CI = 0.82-0.96, p= 0.003). For mild C. difficileinfection, the efficacy of metronidazole and vancomycin resulted in similar clinical cure rates (RR = 0.94, 95% CI = 0.84-1.04, p= 0.21) and sustained cure rates (RR = 0.93, 95% CI = 0.83-1.05, p= 0.26). For severe C. difficileinfection the efficacy of vancomycin was superior to metronidazole in terms of clinical cure rates (RR = 0.81, 95% CI = 0.69-0.95, p= 0.009), whereas sustained cure rates were similar (RR = 0.86, 95% CI = 0.72-1.02, p= 0.08). Regarding microbiological cure metronidazole therapy was as effective as vancomycin therapy (RR = 0.88, 95% CI = 0.64-1.21, p= 0.43). Recurrence rates with metronidazole and vancomycin for both mild C. difficileinfection (RR = 0.95, 95% CI = 0.56-1.60, p= 0.85) and severe C. difficileinfection (RR = 1.27, 95% CI = 0.85-1.91, p= 0.25) were not different. Likewise, no difference in all-cause mortality was found as well (RR = 0.87, 95% CI = 0.56-1.35, p= 0.53). In conclusion, vancomycin provides improved initial clinical and sustained cure rates in patients with C. difficileinfection compared with metronidazole, especially in patients with severe C. difficileinfection. In view of these data, vancomycin may be considered first line therapy for severe C. difficileinfection.