Abstract The elaboration of fruit blends added of functional components results in nutritionally enriched products. The purpose of this work was to evaluate the physicochemical, sensorial and rheological properties of cashew-apple and araça-boi beverages added with encapsulated omega-3 and stability during 120 days of storage. Juice formulations were prepared with and without adding omega-3 oil and encapsulated omega-3, pasteurized and stored at room temperature, and evaluated sensory, physicochemical and rheological properties. The optimized formulation of the beverage was obtained with 18.6% of cashew-apple and 9.3% of araça-boi pulps, selected in the experiment of the response surface. The formulations with encapsulated omega-3 maintained sensory acceptability. The physicochemical parameters did not present significant interactions among the formulations and the storage time. A consumer test with 100 people was performed using an acceptance test and check all that apply (CATA). The beverage added with unencapsulated omega-3 presented a variation in the sensory attributes along with the storage. All samples showed non-Newtonian behavior and pseudoplastic character. Omega-3 encapsulation is recommended to favor the acceptability and constancy of rheological characteristics in the beverage nutritionally enriched with omega-3.

Abstract A cashew nut-based beverage was obtained using broken cashew kernels and its composition and stability were evaluated during refrigerated storage for 60 days. Sensory tests were performed to define the formulation, testing the best proportion between kernels and water, the effect of kernel roasting, the ideal sweetness, the beverage attributes and the sensory acceptance. A cashew nut beverage can be obtained by grinding the kernel with water in the proportion of 1:10 (m/m) with added sugar at 3%, followed by heat treatment at 140ºC for four seconds. The proximate composition was 11.49% total solids, 0.26% ashes, 1.83% proteins, 3.97% lipids and 5.43% total carbohydrates. The pH remained above 6.49 and the color darkened during storage. The product was considered microbiologically safe and shelf-stable for at least 2 months, and sensory acceptance remained at an average value of 7.1 on a 9-point hedonic scale.

Resumo O tamarindo é uma fruta tropical de grande importância para a agricultura familiar e pode ser utilizada como matéria-prima para suco pronto para beber. No entanto, existem poucos dados na literatura sobre sua estabilidade. Este trabalho teve como objetivo avaliar a estabilidade do suco de tamarindo durante o armazenamento em temperatura ambiente (~28 °C) por 180 dias. Duas formulações foram produzidas, sendo um tratamento controle (pH 2,5) e um suco com acidez parcialmente reduzida (pH 3,5). Análises físico-químicas (pH, acidez e sólidos solúveis), de cor, sensoriais e microbiológicas foram realizadas para ambos os sucos, durante o armazenamento. Os resultados mostraram que os sucos foram estáveis em relação a todos os parâmetros físico-químicos analisados. Além disso, mantiveram escores de aceitação global estáveis, apesar de pequenas mudanças nos atributos − gosto de tamarindo, ácido e doce − para o suco parcialmente neutralizado (pH 3,5). Os sucos apresentaram também estabilidade microbiológica no período analisado, o que indicou um processo de fabricação e tratamento térmico eficientes. Portanto, ambos os sucos podem ser armazenados à temperatura ambiente, sem apresentar perda de qualidade.

Abstract Tamarind is a tropical fruit with relevant importance for family agriculture, and can be used as raw material for a ready-to-drink juice; however, there are few data in the literature associated with the tamarind juice storage stability. This work aimed to evaluate the physicochemical, sensory and microbiological stability of tamarind juice throughout storage at room temperature (~28 °C) for 180 days. Two juice formulations were produced, a control (pH 2.5), and the formulation with acidity reduction (pH 3.5). Physicochemical (pH, acidity, and soluble solids), sensory and microbiological analyses were performed for both juices along storage. The results showed that both juices were stable regarding all physicochemical parameters analyzed. Furthermore, both juices kept sensory scores in the acceptance zone, despite little changes in attributes (tamarind, sour, and sweet taste) for the partially neutralized juice (pH 3.5). Microbiological results were also stable for the period analyzed, which indicated efficient good manufacturing process alongside efficient heat treatment. Therefore, both juices can be stored at room temperature without presenting quality loss.

Abstract Cashew nut milk was evaluated as a matrix to deliver commercial probiotic strains (Bifidobacterium animalis, Lactobacillus acidophilus and Lactobacillus plantarum) in order to develop a non-dairy probiotic beverage. The first step was to evaluate the viability of the probiotic strains. All evaluated bacteria were able to survive in this food matrix under 4 °C for 30 days. So, B. animalis was the selected strain to perform a stability test of the beverage by monitoring the pH, color, sensory and microbiological quality during refrigerated storage for 30 days. During storage, B. animalis counts were above 107 CFU.mL-1, the microbiological quality was maintained and there was no significant difference in whiteness. A significant pH decrease was observed, but it did not affect the beverage’s sensory acceptance, indicating that cashew nut milk is an adequate vehicle for delivering probiotics.

Abstract We investigate the impact of yacon syrup consumption on glycemic, lipid and metabolic endotoxemia in healthy subjects. Thus, 32 healthy were randomized into two groups that consumed 40 g of yacon syrup/day (= 8.74 g FOS/day) or a placebo, for 2-weeks. Anthropometric assessments, gastrointestinal effects, glycemic and lipid profile, and LPS were performed at the beginning and at the end of the study. The results obtained shown that the 2-weeks consumption of yacon syrup does not promote serum LPS alteration in healthy volunteers. Moreover, the glycemic and lipid profile were also similar between the groups before and after the intervention. Yacon syrup did not affect blood lipid, glucose or metabolic endotoxemia. However, it is possible that positive effects of yacon syrup will turn evident only in a long-term intervention. Further studies are needed to assess the long-term effect of yacon syrup consumption, and its use in obese and diabetic individuals.

Abstract The aim of this study was to evaluate the antioxidant and prebiotic effect of a beverage composed by tropical fruits and yacon in alloxan-induced diabetic rats. To obtain the beverage, a Response Surface Methodology was used, with concentration of yacon extract (x1) and the sweetener (x2) added to a mixture of tropical fruits as independent variables, and the sensorial acceptance as response. The optimized beverage showed higher values for antioxidant capacity, measured by ABTS, FRAP and DPPH assays. Thus, the fructooligosaccharides content (2.32 ± 0.65 g FOS/100 g), total phenolic (126.83 ± 9.48 mg gallic acid equivalent/100 g) and ascorbic acid (171.64 ± 7.31 mg/100 g) shows that the beverage contains high levels of these bioactive compounds. In addition, the beverage was tested in vivo, using male Wistar rats, divided into five groups (control, non-treated diabetic, and diabetic treated with 100, 200 or 400 mg of lyophilized beverage/kg/day). The results showed a promotion of the growth of lactobacilli in cecal material and an increase in catalase activity in the liver, in a dose-dependent manner, showing the prebiotic and antioxidant effects of the beverage in the diabetic model.

RESUMO O objetivo deste trabalho foi o estudo de estabilidade da polpa de manga em pó obtida pelo processo de liofilização, assim como das suas características estruturais através de análise de microscopia eletrônica. Em resposta ao delineamento experimental realizado precedentemente utilizando concentrações de maltodextrina de 0%; 10%; 20% e 30% e tempos de 24 e 30 horas, foi escolhida a condição de 20% de maltodextrina e 24 horas de secagem como a melhor para secagem de manga em liofilizador. Portanto, o estudo de estabilidade foi realizado com a polpa de manga em pó obtida a partir desta melhor condição de secagem armazenada durante 90 dias, com acompanhamento quinzenal através de realização das análises de umidade, teor de ácido ascórbico, parâmetro colorimétrico b* e higroscopicidade. O armazenamento foi feito a temperatura ambiente (27 ºC) em embalagem laminada com e sem vácuo, e plástica sem vácuo. A embalagem laminada a vácuo apresentou maior manutenção das características iniciais do produto armazenado, principalmente para o teor de ácido ascórbico, higroscopicidade e parâmetro de cor b*. Posteriormente foi realizado o estudo da microestrutura da amostra em diferentes concentrações de maltodextrina (0%; 10%; 20% e 30%) com finalidade de compreender a interferência do adjuvante no produto final. As imagens aproximadas do pó revelaram a formação de superfície porosa e a influência do adjuvante nas dimensões da partícula, na qual maiores concentrações de maltodextrina resultaram em pós de menores e mais porosos.

ABSTRACT The aim of this work was to study the stability of powdered mango pulp obtained through the process of lyophilisation, as well as its structural characteristics using electron microscopy analysis. In response to an earlier experimental design using maltodextrin concentrations of 0%, 10%, 20% and 30% and periods of 24 and 30 hours, the condition of 20% maltodextrin and 24 hours drying was chosen as the best for drying mango in a freeze dryer. The stability study was therefore carried out using powdered mango pulp obtained under the above best-drying conditions, stored for 90 days and monitored every two weeks by analysis of the moisture, ascorbic acid content, b* colorimetric parameter and hygroscopicity. Storage was at room temperature (27 °C) in vacuum and non-vacuum laminated packaging, and in non-vacuum plastic packaging. The vacuum laminated packaging maintained the initial characteristics of the stored product for longer, especially the ascorbic acid content, hygroscopicity and b* colour parameter. The microstructure of the sample was then studied under different concentrations of maltodextrin (0%, 10%, 20% and 30%) so as to understand the effect of the drying agent on the final product. Close-up images of the powder revealed the porous surface formation and the effect of the agent on particle size, where higher concentrations of maltodextrin resulted in more porous powders with smaller particles.

Resumo O objetivo do presente trabalho foi avaliar a estabilidade de uma bebida de caju e yacon durante o armazenamento sob refrigeração. A bebida, composta por polpa de caju e extrato de yacon (proporção 1:1, v/v), foi pasteurizada a 85 °C por 90 segundos e armazenada sob refrigeração (5 °C). No tempo inicial e a cada 45 dias do período de estocagem, foram determinados os teores de compostos bioativos (ácido ascórbico - AA e polifenóis extraíveis totais - PET), a atividade antioxidante total (AAT), os sólidos solúveis totais (SST), a acidez titulável (AT), o pH e a cor (L*, a* e b*). Após 225 dias de estocagem, a bebida apresentou diminuição expressiva da sua AAT, medida pelo método FRAP (Ferric Reducing Antioxidant Power), de aproximadamente 22%, assim como houve queda nas concentrações de AA e PET, em torno de 20 e 16%, respectivamente. A bebida atendeu ao padrão estabelecido pela legislação brasileira para coliformes a 45 °C e Salmonella sp. (ausência/25 mL); porém, em 135 dias de armazenamento, a bebida apresentou contagem de bolores e leveduras superior a 104 UFC/mL. Neste sentido, embora a bebida tenha apresentado níveis consideráveis de compostos bioativos e AAT durante os 225 dias de armazenamento refrigerado (5 °C), recomenda-se que o produto seja armazenado por até 90 dias sob refrigeração (5 °C), período em que a bebida manteve seus componentes bioativos e boa qualidade microbiológica e sensorial.

Abstract The objective of the present study was to evaluate the stability of a beverage produced with cashew apple and yacon during refrigerated storage. The beverage, composed of cashew pulp and yacon extract (ratio 1: 1, v / v), was pasteurized at 85 °C for 90 seconds and stored at 5 °C. The following components were evaluated at zero time (after processing and packaging) and every 45 days during the storage period: the bioactive compound contents (ascorbic acid (AA), total extractable polyphenols (PET), total antioxidant activity (AAT), total soluble solids, titratable acidity (AT), pH and colour (L *, a * and b *). After 225 days of storage, the beverage presented a 22% decrease in AAT as analysed by the FRAP method (Ferric Reducing Antioxidant Power), as well as a drop in the AA and PET concentrations of around 20 and 16%, respectively. The beverage complied with the microbiological standard established by Brazilian legislation for coliforms at 45 °C and Salmonella sp. (absence/25 mL), but after 135 days storage, the beverage had a yeast and mould count greater than 104 CFU/mL. Thus although the beverage presented considerable levels of bioactive compounds and AAT during the 225 days of refrigerated storage (5 °C), it is recommended that it be stored for up to 90 days under refrigeration (5 °C), considering the microbiological, sensorial quality and maintenance of its bioactive components during this period.

The development of beverages with functional properties must consider the preservation of the bioactive or functional properties during storage. For this reason, the aim of this study was to evaluate the stability of a functional beverage of tropical fruits and yacon, stored under refrigeration. The beverage, composed by 50% of yacon and 50% of a blended tropical fruits (camu-camu, acerola, cashew-apple, yellow mombin, acai and pineapple), was pasteurized (90 seconds/ 85°C) and stored under refrigeration (5°C). After processing and on 45 day intervals until the end of storage, were assayed the bioactive compounds (ascorbic acid and total extractable polyphenols), antioxidant activity, total soluble solids, titratable total acidity, pH, color (L*, a* and b*), total sugar content, sucrose, glucose and fructose, and nd the physical and chemical analyzes were limited by decreased total antioxidant activity and their bioactive components. The beverage showed relative physical and chemical quality during storage period , and in the 225 days of storage, the total extractable polyphenols and total antioxidant activity showed a significantly decline , and thus , these parameters were evaluated only until this period. However, the main limitation for the beverage storage was due to sensory acceptability and microbiological safety, which although in accordance with Brazilian legislation, limited storage period for 90 days.

O desenvolvimento de bebidas com perfil funcional deve considerar também a conservação dos compostos ou propriedades funcionais durante o período de armazenamento. Assim, o objetivo do presente trabalho foi avaliar a estabilidade de uma bebida funcional de frutas tropicais e yacon durante o armazenamento em refrigeração (5°C). A bebida, composta por 50% de extrato de yacon e 50% de blend de frutas tropicais (camu-camu, acerola, caju, cajá, açaí e abacaxi) foi pasteurizada (90 segundos/85°C) e armazenada sob refrigeração (5°C). No tempo inicial, e a cada 45 dias do período de estocagem, foram determinados os compostos bioativos (ácido ascórbico e polifenóis extraíveis totais), atividade antioxidante total, sólidos solúveis totais, acidez total titulável, pH, cor (L*, a* e b*), açúcares totais, sacarose, glicose e frutose, sendo que a análises físicas e químicas foram limitadas pela diminuição da atividade antioxidante total e de seus componentes bioativos. A bebida manteve relativa qualidade física e química durante o período de armazenamento, sendo que em 225 dias de estocagem, os teores de polifenóis extraíveis totais e atividade antioxidante total apresentaram uma queda mais acentuada, e desta forma, estes parâmetros foram avaliados somente até este período. Porém, o principal limitante para o armazenamento da bebida foi devido a aceitabilidade sensorial e segurança microbiológica, que embora de acordo com a legislação vigente, limitou o período de armazenamento para 90 dias.

El objetivo de este trabajo fue investigar los efectos sinérgicos, aditivos y antagónicos de mezclas de diferentes frutas tropicales en la capacidad antioxidante total (TAC) y compuestos bioactivos presentes en los jugos mixtos, y optimizar su formulación por la metodología de superficie de respuesta basado en las evaluaciones de: polifenoles totales (TP), capacidad antioxidante total (TAC), contenido de ácido ascórbico y la aceptación sensorial. Camu-camu, acerola y acai fueron las frutas que más influyeron en el potencial antioxidante del jugo mixto; y el jobo mostró un efecto positivo en la aceptación del jugo mixto tropical. Se observó un efecto antagónico entre acerola y camu-camu para la TAC. La formulación óptima obtenida contenía 20% acerola, 10% de camu-camu, 10% el jobo, 10% de manzana de marañón y 10% de acai, la cual ha proporcionado contenidos medio de 155,46 mg.100 g-1 de ácido ascórbico, 103,01 mg de GAE.100 g-1 de TP, 10,27 mM Trolox g-1 de TAC y aproximadamente 6.1 de aceptación sensorial.

The objective of this work was investigate the synergistic, additive and antagonistic effects of fruit mixtures on total antioxidant capacities and bioactive compounds in tropical fruit juices, and optimize its formulation by the response surface methodology based on the responses: total polyphenols (TP), total antioxidant capacity (TAC), ascorbic acid content and sensorial acceptance. Camu-camu, acerola and acai were the major factors that influenced the antioxidant potential of the juice; and the yellow mombin showed a positive effect on the acceptance of the tropical juice. It was observed an antagonistic effect between acerola and camu-camu for the TAC response. The optimum formulation obtained was 20% acerola, 10% camu-camu, 10% yellow mombin, 10% cashew apple and 10% acai, which was responsible for a response of 155.46 mg.100 g-1 of ascorbic acid, 103.01 mg of GAE.100 g-1 of TP, 10.27 μM Trolox g-1 of TAC and approximately 6.1 of acceptance.