Abstract Background: Primary cutaneous CD4+ small/medium-sized pleomorphic T-Cell lymphoproliferative disorder (PC-SMTLD) has been considered as a controversial dermatological disease that has been included in cutaneous T-cell lymphoma group, presenting most commonly as a solitary nodule and/or plaque with a specific and characteristic head and neck predilection. Due to the considerable overlap between PC-SMTLD and pseudolymphoma (PL), the differential diagnosis is often challenging. Methylation of DNA at position 5 of cytosine, and the subsequent reduction in intracellular 5-hydroxymethylcytosine (5-hmC) levels, is a key epigenetic event in several cancers, including systemic lymphomas. However, it has rarely been studied in cutaneous lymphomas. Objectives: The authors aimed to explore the role of differential 5-hmC immunostaining as a useful marker to distinguish PC-SMTLD from PL. Methods: Retrospective case series study with immunohistochemical and immunofluorescence analysis of 5-hmC was performed in PL and PC-SMTLD. Results: Significant decrease of 5-hmC nuclear staining was observed in PC-SMTLD when compared with PL (p<0.0001). By semi-quantitative grade integration, there were statistical differences in the final 5-hmC scores in the two study groups. The IF co-staining of 5-hmC with CD4 revealed a decrease of 5-hmC in CD4+ lymphocytes of PC-SMTLD. Study limitations: The small clinical sample size of the study. Conclusions: The immunorreactivity of 5-hmC in CD4+ lymphocytes was highly suggestive of a benign process as PL. Furthermore, the decrease of 5-hmC nuclear staining in PC-SMTLD indicated its lymphoproliferative status and helped to make the differential diagnosis with PL. © 2023 Sociedade Brasileira de Dermatologia. Published by Elsevier España, S.L.U. This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/).

Abstract Based on the inverse piezoelectric effect and other characteristics of piezoelectric ceramics, a new type of piezoelectric variable friction damper is developed, and the output performance test of piezoelectric ceramic actuator and piezoelectric variable friction damper is carried out, and the parametric mechanical model of the damper is established by using Simulink to carry out numerical simulation, and compared and analyzed with the test results. The results of the study show that the simulation and the test present a good regular agreement. With the increment of loading voltage, the displacement deformation and zero-displacement thrust of piezoelectric ceramic actuator are approximately linear with the loading voltage; when the loading voltage is increased by 15V every time, the test value of the output damping force of the damper increases by 259.66N on average step by step, and the simulated value increases by 256.14N step by step, and the damping force has a linearly incremental relationship with the voltage and the slope of the linear increase is 16.434.

ABSTRACT: To investigate the development and dynamics of green manure research, we collected 802 and 4588 papers on green manure research from China national knowledge infrastructure (CNKI) and Web of Science (WoS) between 1992 and 2021, respectively, and conducted quantitative and visual analyses based on bibliometric methods on the annual number of publications, authors and institutions, keywords, and emergent words. The main research hotspots in the field of green manure are discussed and summarized based on the results of the visualization analysis and the important publications explored by the results of the visualization analysis. The results showed that: the overall number of publications on green manure research exhibits an increasing trend, and research in this field is receiving more and more attention; the United States is the core country in this research field, with a great influence; although, the number of Chinese publications is large, the intermediary centrality value is small and the level of scientific research needs to be improved; the research characteristics in the field of green manure research mainly focus on green manure cultivation technology, the effects on soil and crops, and the effects on pests and diseases. The study was conducted based on CiteSpace, a visualization tool of bibliometrics, with comprehensive literature information and intuitive results, providing important references for relevant researchers to understand the extent of research in this field, academic communication, and grasp research dynamics.

RESUMO: Para investigar o desenvolvimento e a dinâmica da pesquisa de adubo verde, coletamos 802 e 4.588 artigos sobre pesquisa de adubo verde da infraestrutura nacional de conhecimento da China (CNKI) e da Web of Science (WoS) entre 1992 e 2021, respectivamente, e realizamos estudos quantitativos e visuais. Análises baseadas em métodos bibliométricos sobre o número anual de publicações, autores e instituições, palavras-chave e palavras emergentes. Os principais focos de pesquisa na área de adubação verde são discutidos e resumidos com base nos resultados da análise de visualização e nas importantes publicações exploradas pelos resultados da análise de visualização. Os resultados mostram que: o número total de publicações sobre a pesquisa de adubação verde apresenta uma tendência crescente, e as pesquisas nessa área estão recebendo cada vez mais atenção; os Estados Unidos são o país central neste campo de pesquisa, com grande influência, embora o número de publicações chinesas seja grande, o valor de centralidade intermediária seja pequeno e o nível de pesquisa científica precise ser melhorado; as características da pesquisa no campo da pesquisa de adubo verde concentram-se principalmente na tecnologia de cultivo de adubo verde, nos efeitos sobre o solo e as culturas e os efeitos sobre pragas e doenças. O estudo foi realizado com base no CiteSpace, ferramenta de visualização da bibliometria, com informações bibliográficas abrangentes e resultados intuitivos, fornecendo referências importantes para pesquisadores relevantes compreenderem a extensão da pesquisa neste campo, a comunicação acadêmica e a compreensão da dinâmica da pesquisa.

Abstract Objective This retrospective study aimed to analyze the clinical efficacy of two regenerative surgical methods — Bio-Oss granules combined with barrier membranes and Bio-Oss Collagen alone — and to help clinicians achieve better periodontal regeneration outcomes in the specific periodontal condition. Methodology Patients who underwent periodontal regeneration surgery from January 2018 to April 2022 were retrospectively screened, and their clinical and radiographic outcomes at 6 months postoperatively were analyzed. The probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), gingival recession (GR), distance from the cemento-enamel junction to the bottom of the bone defect (CEJ-BD), and depth of intrabony defects (INFRA) were recorded before the operation (T0) and 6 months after it (T1), and subsequently compared. Results In total, 143 patients were included — 77 were placed in the Bio-Oss group and 66 were placed in the Bio-Oss Collagen group. All indicators, including PD and CAL at T1, showed significant differences compared to baseline, for both groups (P<0.001). PD reduction was greater in the group receiving the Bio-Oss Collagen treatment (P=0.042). Furthermore, in cases when the baseline PD range was 7-11 mm and the age range was 35-50 years, PD reduction was more significant for patients receiving the Bio-Oss Collagen treatment (P=0.031, 0.023). A linear regression analysis indicated that postoperative PD and CAL were positively correlated with baseline values, and that the efficacy tended to decrease with increasing age. Conclusion Both the use of Bio-Oss Collagen alone and the use of Bio-Oss granules combined with barrier membranes resulted in significant effects in the treatment of periodontal intrabony defects. The Bio-Oss Collagen treatment generated more improvements in PD than the Bio-Oss granules combined with barrier membranes, particularly within the baseline PD range of 7-11 mm and the 35-50 years age group. Additionally, age was the main factor influencing the effectiveness of regenerative surgery for intrabony defects: older individuals exhibited fewer improvements.

Abstract In this paper, the penetration resistance of 6061 aluminum plates to explosively formed projectiles (EFPs) at the incidence angles of 0°, 15°, 30°, and 45° was studied. The penetration process was roughly divided into three typical stages—the plastic flanging stage of the target, the righting stage of the projectile, and the plug formation stage of the target. Through the energy analysis, the energy consumption of the aluminum plate increased with the increase in the incidence angle during the entire penetration process. Through the microscopic analysis of the perforated wall surface of the aluminum plate, it was found that there were distinct boundaries and differences between the plastic reaming zone and the punching plug fracture zone.. Overall, four kinds of classification modes were proposed for the damage of the aftereffect plate, which provides a method to evaluate the aftereffect of EFP oblique penetration into 6061 aluminum plates.

PURPOSE: The study explores the U-shaped impact of responsible leadership on cyberloafing and the moderating effect of self-efficacy based on conservation of resources theory. DESIGN/METHODOLOGY/APPROACH: This is a field study based on quantitative analysis of full-time employees in the enterprises who work on computers for over 1 year. Hierarchical regression was employed to examine hypotheses. Bootstrap and Bayesian analysis to conduct robustness tests. FINDINGS/RESULTS: Results from 305 employees showed that responsible leadership had a U-shaped impact on cyberloafing. The self-efficacy moderated the U-shaped relationship between responsible leadership and cyberloafing. For employees with high self-efficacy, responsible leadership is more likely to reduce cyberloafing, but for employees with low self-efficacy, responsible leadership is more likely to increase cyberloafing. PRACTICAL IMPLICATIONS: Organisations should recognise the role of leaders in mitigating employees' cyberloafing behaviour through effective supervision, and equip the leaders with a comprehensive understanding of their impact. Besides, for employees with low self-efficacy, leaders should pay attention and help them through effective supervision. ORIGINALITY/VALUE: Our work illustrated the double-edged sword effect of responsible leadership on employee cyberloafing and identified self-efficacy as a key boundary condition of the conservation of resources theory. This study expands the related research on the influencing factors of cyberloafing behaviour and provides clue for the possible negative effects of responsible leadership. In addition, this study deepens the conservation of resources theory.

Abstract Objective: The objective of this study was to investigate the effects of activity-based locomotor training (ABLT) on motor function and walking ability in children with spinal cord injury (SCI). Materials and methods: The Chinese National Knowledge Infrastructure, WanFang, VIP, PubMed, and Web of Science databases were searched for related studies, with two reviewers subsequently evaluating the literature quality using the Cochrane Handbook. Results: A total of 11 studies were eligible, while only one met the ABLT standard program criteria. Overall, ABLT significantly improved the lower limb motor function, increased walking speed and distance, and improved the daily living ability of children with SCI. Conclusions: The ABLT strategy is of great significance to the motor function and walking ability of children with SCI. At present, there exist few studies on the application of ABLT for pediatric SCI. Further control studies with a larger sample size are required to improve the ABLT program guidelines for children with SCI.

Resumen Objetivo: Discuta el impacto del entrenamiento ejercicio basado en la actividad en la lesión de la médula espinal en la función de movimiento de los niños y la capacidad de caminar. Materials y métodos: Según China Zhiwang, Wanfang, VIP, PubMed, Science Network y otros documentos relacionados como fuente de datos. Dos revisores usan calidad de evaluación manual de Cochrane. Resultados: Un total de 11 estudios cumplen con las condiciones. Solo hay un estudio que cumple con los proyectos estándar de ABLT. General, ABLT mejora significativamente la función de los niños con lesiones de la médula espinal, aumenta la velocidad y la distancia de caminar y mejora la capacidad de la vida diaria. Conclusión: La estrategia ABLT es de gran importancia para la función de movimiento de los niños de la médula espinal y la capacidad de caminar. En la actualidad, ABLT tiene menos investigación en lesión pediátrica de la médula espinal. Es necesario mostrar la cantidad de muestra y controlar la investigación para mejorar las pautas del plan ABLT para el daño de la médula espinal a los niños.

Abstract Introduction: Propofol is a widely used anesthetic and its dose is closely related to aging. Telomere length (TL) is a unique heritable trait, and emerging as a biomarker of aging, health and disease. Telomerase RNA component (TERC) plays an important role in maintaining TL. We proposed a hypothesis that propofol dose in general anesthesia can be predicted by measuring TL before operation, which greatly reduced the risk of anesthesia, especially the elderly. Methods: The association between the propofol dose in anesthesia induction and: TL in the DNA of peripheral blood leukocytes; body weight; sex; difference of the Bispectral Index (BIS) before and after anesthesia induction in patients was evaluated by multivariable linear regression analyses. The mutation at the 5’end or 3’end of TERC was detected. We recruited 100 patients of elective surgery. Results: We found that propofol dose in anesthesia induction was clearly correlated significantly with TL (r = 0.78, p < 0.001), body weight (r = 0.84, p = 0.004), sex (r = 0.83, p= 0.84, p = 0.004), sex (r = 0.83, p = 0.004), and difference of BIS before and after anesthesia induction (r = 0.85, p = 0.029). By comparing the absolute values of standardized regression coefficients (0.58, 0.21, 0.19, and 0.12) of the four variables, it can be seen that TL contributes the most to the propofol dose in anesthesia induction. However, the mutation at the 5’ end or 3’ end of TERC was not found. Conclusions: These findings provide preliminary evidence that the propofol dose in anesthesia induction was clearly correlated with genetically determined TL. TL may be a promising predictor of the propofol dose, which is beneficial to improve the safety of anesthesia and reduce perioperative complications.

ABSTRACT Broccoli is increasingly favored by consumers as it is rich in vitamins and many other bioactive compounds. However, existing large tractor-drawn vegetable harvesting machinery is more suitable for large farmland and farm cultivation patterns. Therefore, this paper develops tracked broccoli harvesting equipment for small farmland and tests the ability to harvest broccoli. The tracked broccoli harvester developed in this paper is capable of traveling on its own in the field, cutting the stalks and transporting the broccoli bulbs. This paper analyses the working process of the cutting device and designs the working parameters. Through the cutting bench experiment, the optimal working parameters of the cutting device were determined as 456 r/min of rotational speed, 8.312 mm of overlap, and 0.255 m/s of pusher speed. In the field test, the broccoli leakage rate was 4.8%, the cutting qualification rate was 81.6%, the transport qualification rate was 95.62%, the damage rate was 3.96%, and the total loss rate was 8.55%. This structural model is an important technical support for broccoli harvesting equipment in China.

Resumen Antecedentes: La enfermedad de injerto contra huésped (EICH) es una complicación importante después del trasplante alogénico de células madre hematopoyéticas. Objetivos: Para dilucidar el papel de TLR4, el principal receptor de LPS bacteriano, en el desarrollo de GVHD, construimos un modelo de GVHD en ratones knockout para TLR4 (TLR4-/-) y monitoreamos el quimerismo celular. Métodos: En este estudio, usamos PCR para identificar si se establecieron ratones knockout para TLR4 (TLR4-/-). Antes del trasplante, pretratamos a los ratones con irradiación para obtener la dosis de irradiación adecuada. Se aplicó citometría de flujo para medir el estado de quimerismo, las distribuciones de APC y células T en ratones receptores TLR4+/+ y TLR4-/-. Resultados: El estado general de los receptores de TLR4-/- fue mejor que el de los receptores de TLR4+/+, y los ratones receptores de TLR4-/- mostraron manifestaciones de GVHD menos graves que los ratones receptores de TLR4+/+. La mayoría de las APC y las células T en el bazo del ratón huésped se derivaron de las células del donante, y las células T CD4+, incluidas las células T de memoria, se encontraban en su mayoría en los ratones huéspedes. Conclusión: En general, nuestros datos muestran que la eliminación de TLR4 atenuó el desarrollo de GVHD, lo que sugiere que TLR4 podría usarse como un nuevo objetivo y paradigma terapéutico en las terapias de GVHD.

Abstract Background: Graft-versus-host disease (GVHD) is a major complication after allogeneic hematopoietic stem cell transplantation. Objective: To elucidate the role of Toll-like receptor 4 (TLR4), the major receptor for bacterial lipopolysaccharide, in the development of GVHD, we constructed a GVHD model in TLR4 knockout (TLR4-/-) mice and monitored the cell chimerism. Methods: In this study, we used polymerase chain reaction to identify whether TLR4 knockout (TLR4-/-) mice were established. Before transplantation, we pretreated mice with irradiation so as to obtain an appropriate irradiation dose. Flow cytometry was applied to measure the chimerism status, the distributions of antigen-presenting cells (APCs), and T-cells in TLR4+/+ and TLR4-/- recipient mice. Results: The general condition of TLR4-/- recipients was better than that of TLR4+/+ recipients, and the TLR4-/- recipient mice showed less severe GVHD manifestations than the TLR4+/+ recipient mice. Most of the APCs and T-cells in the host mouse spleen were derived from donor cells, and CD4+ T-cells, including memory T-cells, were in the majority in host mice. Conclusion: In general, our data show that TLR4 deletion attenuated GVHD development, which suggests that TLR4 could be used as a novel target and therapeutic paradigm in GVHD therapies.

Abstract Objective: to explore the association between serum vitamin D level and the occurrence and pathological grade of gastric cancer. Material and methods: search PubMed, Embase, Web of Science, Cochrane and Chinese database; all articles about the association between serum vitamin D levels and gastric cancer published before July 2021. Results: 10 trials with 1159 cases of gastric cancer patients and 33,387 cases of regular control patients were analyzed. The serum vitamin D level of the gastric cancer group (15.56 ± 7.46 ng/ml) was lower than in the control group (17.60 ± 1.61 ng/ml), and the difference was statistically significant. The patients with gastric cancer, clinical stage III/IV (16.19 ± 8.04 ng/ml) had lower vitamin D levels than those with stage I/II (19.61 ± 9.61 ng/ml), and the patients with low differentiation of gastric cancer (17.5 ± 9.5 ng/ml) had lower levels than those with well- or moderately-differentiated cancer (18.04 ± 7.92 ng/ml). The patients with lymph node metastasis (19.41 ± 8.63 ng/ml) had lower vitamin D levels than the patients without lymph node metastasis (20.65 ± 7.96 ng/ml), and the difference was statistically significant. Conclusion: vitamin D levels were negatively associated with gastric cancer. Vitamin D levels were significantly associated with different clinical stages, degrees of differentiation, and lymph node metastasis, suggesting that low vitamin D levels might predict poor prognosis in gastric cancer.

Resumen Objetivo: investigar la asociación entre los niveles de vitamina D en suero y la carcinogénesis gástrica y su clasificación patológica. Material y métodos: se buscaron en las bases de datos PubMed, Embase, Web of Science, Cochrane y China todos los artículos sobre la asociación entre los niveles séricos de vitamina D y el cáncer gástrico publicados antes de julio de 2021. Resultados: se analizaron diez datos de 1159 pacientes con cáncer gástrico y 33.387 pacientes normales de control. El nivel de vitamina D en suero del grupo (15,56 ± 7,46 ng/ml) de cáncer gástrico era inferior al del grupo de control (16,19 ± 8,04 ng/ml), y la diferencia era estadísticamente significativa. En los pacientes con cáncer gástrico en estadio clínico III/IV (16,19 ± 8,04 ng/ml) era inferior al de los pacientes en estadio I/II (19,61 ± 9,61 ng/ml) y en los pacientes con cáncer de estómago poco diferenciado (17,5 ± 9,5 ng/ml) era menor que en los pacientes con cáncer bien o moderadamente diferenciado (18,04 ± 7,92 ng/ml); en los pacientes con metástasis en ganglios linfáticos (19,41 ± 8,63 ng/ml) era inferior al de los pacientes sin metástasis en ganglios linfáticos (20,65 ± 7,96 ng/ml), siendo la diferencia estadísticamente significativa. Conclusión: los niveles de vitamina D se correlacionaron negativamente con el cáncer gástrico. Los niveles de vitamina D se asociaron significativamente a los diferentes estadios clínicos, el grado de diferenciación y la metástasis en ganglios linfáticos, lo que sugiere que los niveles bajos de vitamina D pueden ser un factor de predicción de mal pronóstico en el cáncer gástrico.

ABSTRACT Purpose: To compare the effectiveness and safety of marketed oral drugs for overactive bladder based on a systematic review and network meta-analysis approach. Methods: Pubmed, Embase, Web of Science, and the Cochrane Register of Clinical Trials databases were systematically searched. The search time frame was from database creation to June 2, 2022. Randomized controlled double-blind trials of oral medication for overactive bladder were screened against the protocol's entry criteria. Trials were evaluated for quality using the Cochrane Risk of Bias Assessment Tool, and data were statistically analyzed using Stata 16.0 software. Result: A total of 60 randomized controlled double-blind clinical trials were included involving 50,333 subjects. Solifenacin 10mg was the most effective in mean daily micturitions and incontinence episodes, solifenacin 5/10mg in mean daily urinary urgency episodes and nocturia episodes, fesoterodine 8mg in urgency incontinence episodes/d and oxybutynin 5mg in voided volume/micturition. In terms of safety, solifenacin 5mg, ER-tolterodine 4mg, mirabegron, vibegron and ER-oxybutynin 10mg all showed a better incidence of dry mouth, fesoterodine 4mg, ER-oxybutynin 10mg, tolterodine 2mg, and vibegron in the incidence of constipation. Compared to placebo, imidafenacin 0.1mg showed a significantly increased incidence in hypertension, solifenacin 10mg in urinary tract infection, fesoterodine 4/8mg and darifenacin 15mg in headache. Conclusion: Solifenacin showed better efficacy. For safety, most anticholinergic drugs were more likely to cause dry mouth and constipation, lower doses were better tolerated. The choice of drugs should be tailored to the patient's specific situation to find the best balance between efficacy and safety.

Abstract Lateral Lymph Node Metastasis (LLNM) is common in Papillary Thyroid Carcinoma (PTC) and is associated with a poor prognosis. LLNM without central lymph node metastasis as skip metastasis is not common. We aimed to investigate clinicopathologic and sonographic risk factors for skip metastasis in PTC patients, and to establish a nomogram for predicting the possibility of skip metastasis in order to determine the therapeutic strategy. We retrospectively reviewed the data of 1037 PTC patients who underwent surgery from 2016 to 2020 at a single institution. Univariate and multivariate analyses were used to identify the clinicopathologic and preoperative sonographic risk factors of skip metastasis. A nomogram including the risk factors for predicting skip metastasis was further developed and validated. The incidence of skip metastasis was 10.7%. The univariate and multivariate analyses suggested that gender (p = 0.001), tumor location (p = 0.000), extrathyroidal extension (p = 0.000), and calcification (p = 0.000) were independent risk factors. For papillary thyroid microcarcinoma, tumor location (p = 0.000) and calcification (p = 0.001) were independent risk factors. A nomogram according to the clinicopathologic and sonographic predictors was developed. The receiver operating characteristic curve indicated that AUC was 0.824 and had an excellent consistency. The calibration plot analysis showed a good performance and clinical utility of the model. Decision curve analysis revealed it was clinically useful. A nomogram for predicting the probability of skip metastasis was developed, which exhibited a favorable predictive value and consistency. For the female PTC patient, tumor located at the upper pole is more likely to have skip metastasis. Surgeons and sonographers should pay close attention to the patients who have the risk factors. Evidence level: This article’s evidence level is 3. Level 3 evidence is derived from nonrandomized, controlled clinical trials. In this study, patients who receive an intervention are compared to a control group. Authors may detect a statistically significant and clinically relevant outcome.

Abstract Objectives: The degree of endolymphatic hydrops in Meniere’s disease may be related to hearing loss. However, the results of prior studies have been inconsistent. We aimed to investigate the relationship between endolymphatic hydrops and hearing loss characteristics in Meniere’s disease. Methods: This study included 54 patients (62 ears) with Meniere’s disease. Patients underwent three-dimensional real inversion recovery sequences for magnetic resonance imaging and puretone audiometry. Endolymphatic hydrops were assessed according to Gurkov’s criteria (2011). Correlations between different degrees of endolymphatic hydrops and pure-tone audiometry, as well as staging, were analysed. Results: Pure tone audiometry and staging were higher for vestibular endolymphatic hydrops complicated by cochlear ones than isolated cochlear or vestibular hydrops (both p<0.05). There was no significant correlation between vestibular endolymphatic hydrops and pure-tonal audiometry or staging (all p > 0.05). The degree of hydrops in the middle turn was correlated with the mid-frequency hearing threshold (p<0.05). The degree of cochlear hydrops was correlated with the audiometry, low-frequency hearing thresholds, mid-frequency hearing thresholds and staging (p<0.01). Conclusion: The types and sites of endolymphatic hydrops based on a 3D-real inversion recovery sequence can be used to indicate the degree of hearing loss in patients with Meniere's disease. Level of evidence: Level III.