ABSTRACT Introduction: Pesticides can trigger kidney disease. Objective: To describe the exposure to pesticides of patients with chronic kidney disease on dialysis. Methods: Quantitative and descriptive field research, with 90 patients with chronic kidney disease on dialysis in two hemodialysis units in the state of Santa Catarina, through the application of a structured questionnaire. Participants were divided into two groups: with and without exposure to pesticides. The questionnaire was applied in hemodialysis clinics during treatment. Laboratory test values were collected from clinical records. Data were analyzed using descriptive statistics and association using the chi-square test. For laboratory test data, a comparison of means was performed using the unpaired Student’s t-test between the groups. Results: The mean age of exposed participants was 58 years (±13.7; minimum = 23; maximum = 75) and that of non-exposed participants was 64 years old (±13.9; minimum = 35; maximum = 96). Of the 90 patients, 30% were exposed to pesticides. The mean exposure time was 6.7 ± 3.8 hours/day. There was a statistically significant association between the preparation of the mixture with pesticides and diabetes (p ≤ 0.048). There was no statistically significant difference between the results of laboratory tests in the exposed and non-exposed groups. Conclusion: This study shows that pesticides can be triggering factors for chronic kidney disease (CKD); however, we must expand research in this field to prove the relationship between exposure to pesticides and CKD.

Resumo Introdução: O uso de agrotóxicos pode desencadear doença renal. Objetivo: Descrever a exposição a agrotóxicos de pacientes com doença renal crônica em tratamento dialítico. Métodos: Pesquisa de campo, quantitativa e descritiva, com 90 portadores de doença renal crônica em tratamento dialítico em duas unidades de hemodiálise no estado de Santa Catarina, por meio da aplicação de um questionário estruturado. Os participantes foram divididos em dois grupos: sem e com exposição a agrotóxicos. O questionário foi aplicado nas clínicas de hemodiálise durante o tratamento. Foram coletados valores de exames laboratoriais dos prontuários clínicos. Os dados foram analisados pela estatística descritiva e associação pelo teste qui-quadrado. Para os dados dos exames laboratoriais, foi realizada comparação de médias pelo teste t de Student não pareado entre os grupos. Resultados: A idade média dos participantes expostos foi de 58 anos (±13,7; mínimo = 23; máximo = 75) e a dos não expostos, de 64 anos (±13,9; mínimo = 35; máximo = 96). Dos 90 pacientes, 30% foram expostos a agrotóxicos. O tempo médio de exposição foi de 6,7 ± 3,8 horas/dia. Houve associação estatística significativa entre o preparo da calda com agrotóxicos e a presença de diabetes (p ≤ 0,048). Não houve diferença estatística significativa entre os resultados dos exames laboratoriais do grupo exposto e do não exposto. Conclusão Esta pesquisa evidencia que os agrotóxicos podem ser fatores desencadeadores da doença renal crônica (DRC), entretanto sugere-se ampliar pesquisas na área que possam comprovar a relação entre exposição a agrotóxicos e DRC.

RESUMO: O arroz daninho (Oryza sativa L.) é a principal planta daninha do arroz cultivado e, devido à similaridade genética com a cultura, são poucas as alternativas de controle. Uma possibilidade são os sistemas de cultivo, em razão da influência na emergência e sobrevivência da espécie no banco de sementes do solo. Diante disso, o trabalho objetivou avaliar a longevidade e a distribuição das sementes de arroz daninho no perfil do solo, após 22 anos de cultivo, sob diferentes sistemas. Os tratamentos foram arranjados em esquema fatorial, em que o fator A foi composto por três sistemas de cultivo, realizados consecutivamente durante 22 anos, sendo esses: semeadura direta (SD), sistema convencional (SC) e pré-germinado (PG); e, o fator B composto por quatro profundidades de amostragem: 0-2, 2-5, 5-10 e 10-20 cm. As variáveis avaliadas foram: o número de sementes íntegras e deterioradas m-2; e, a porcentagem de viabilidade. Não foi verificado efeito dos sistemas até 5 cm, mas a 5-10 cm SC e PG mostraram maior quantidade de sementes, e PG a 10-20 cm. A SD proporcionou diminuição de sementes íntegras conforme aumentou a profundidade de amostragem, enquanto o sistema PG elimina menos sementes do banco de sementes do solo. Os sistemas SC e PG distribuem sementes viáveis no perfil do solo de 0 a 20 cm de profundidade. Após 22 anos, nos três sistemas de cultivo há sementes de arroz daninho viáveis até 10 cm de profundidade, sendo igual a quantidade de sementes viáveis até cinco centímetros de profundidade.

ABSTRACT: Weedy rice (Oryza sativa L.) is the most problematic weed in rice fields due to the few control management alternatives to control it, because of the genetic similarity with the crop. Different cropping systems (regarding soil preparation before sowing) have been used as options to control the persistence and emergence of the weed seedbank. Therefore, the objective of this study was to evaluate the longevity and vertical distribution of weedy rice seeds in the soil seedbank after 22 years of different rice cropping systems. Data was analyzed as a two-way factorial, with cropping systems carried out for 22 years [no-tillage (NT), conventional tillage (CT), wet direct-seeded (WDS)] as one factor and sampling depth (0-2, 2-5, 5-10 and 10-20 cm) as the other factor. The number of whole and deteriorated seeds per m-2 were assessed, as well as the viability (%). No effect between the systems were detected up to 5 cm, however at 5-10 cm CT and WDS showed higher amount of seeds, and WDS at 10-20 cm. As the sampling depth increased, NT showed fewer amount of seeds, while less reduction of the soil seedbank was observed in WDS. CT and WDS spread viable seeds in the soil profile from 0 to 20 cm depth. After 22 years there are viable weedy rice seeds up to 10 cm of depth in the three cropping systems and there is no difference among them up to 5 cm of depth, demonstrating the serious problem of the seedbank for this species.

Abstract Improper disposal of expired medicines and treatment leftovers can contaminate the environment and compromise the human health, however, the implementation of reverse logistics for medicines is a challenge for municipalities. The objective of this study was to characterize the medicines disposal by the rural population of the Correia Pinto municipality/SC. Study was quantitative, descriptive-transversal, with data collection in the field from the application of a questionnaire to 156 rural residents. The results showed that basic sanitation is precarious, as 64.7% of people consume untreated water and are not served by waste collection, although 94.9% use the septic tank as sewage treatment. A high percentage of the population (63.43%) reported the daily use of medication. About 75% discard leftovers from drug treatment and expired drugs inappropriately. The data collected can contribute to the implementation strategies of the reverse logistics system for medicines waste, in addition to show the gaps in rural sanitation.

Resumen La disposición inadecuada de medicamentos vencidos y sobrantes de tratamientos puede contaminar el medio ambiente y la salud humana, sin embargo, la implementación de la logística inversa de medicamentos es un desafío para los municipios. El objetivo de este estudio fue caracterizar la disposición de medicamentos por parte de la población rural del municipio de Correia Pinto/SS. La investigación fue cuantitativa, descriptiva-transversal, con recolección de datos en campo a partir de la aplicación de un cuestionario a 156 pobladores de la zona rural. Los resultados mostraron que el saneamiento básico es precario, ya que el 64,7% de las personas consume agua no tratada y no cuenta con recolección de residuos, aunque el 94,9% utiliza la fosa séptica como tratamiento de aguas residuales. Un alto porcentaje de la población (63,43%) refirió el uso diario de medicamentos. Alrededor del 75% desechan los restos del tratamiento farmacológico y los medicamentos vencidos de manera inapropiada. Los datos recopilados pueden contribuir a las estrategias de implementación del sistema de logística inversa para medicamentos domiciliarios, además de mostrar las brechas en el saneamiento rural.

Resumo O descarte inadequado de medicamentos vencidos e sobras de tratamentos podem contaminar o ambiente e comprometer a saúde humana, porém, a implementação da logística reversa para os medicamentos é um desafio aos municípios brasileiros. O objetivo deste estudo foi caracterizar o descarte de medicamentos pela população rural do município de Correia Pinto/SC. A pesquisa foi do tipo quantitativa descritiva-transversal, com coleta de dados a campo a partir da aplicação de um questionário a 156 moradores da área rural. Os resultados mostraram que o saneamento básico é precário, pois 64,7% das pessoas consomem água sem tratamento e não são atendidas pela coleta de resíduos, apesar de 94,9% utilizar a fossa séptica como tratamento do esgoto. Elevado percentual da população (63,43%) relataram a utilização diária de medicamentos. Cerca de 75% descartam as sobras do tratamento medicamentoso e os medicamentos vencidos de forma inadequada. Os dados levantados podem contribuir com as estratégias de implementação do sistema de logística reversa dos medicamentos domiciliares, além de mostrar as lacunas do saneamento básico rural.

Abstract: The objective was to evaluate the management of waste from health services by dentists who work in public and private networks. The study was a field, quantitative, and descriptive research with 48 public and private network professionals. Data collection took place between December 2020 and March 2021, through the application of a questionnaire with 40 questions, which was disseminated in social networks and applied via Google Forms after approval by the Research Ethics Committee. Data were submitted to descriptive statistics and the association test between variables (chi-square). It was observed that 29.17% of the participants are not aware of the disposal of Health Service Waste (RSS), 39.58% are unaware of the disposal procedures for expired medicines and their leftovers, 47.92% are unaware of the Health Service Waste Management Plan, 45.83% are unaware of how long it has been implemented, and 77.08% stated lack of training on RSS management, as the main difficulty pointed out for adequate waste management. It is essential to promote permanent education practices that contribute to training and professional performance on RSS management and instigate the implementation and operation of the municipal waste management plan.

Resumen: El objetivo fue evaluar la gestión de resíduos de los servicios de salud por parte de los odontólogos que trabajan en redes públicas y privadas. El estudio fue de campo, cuantitativo y descriptivo, con 48 profesionales de redes públicas y privadas. La recopilación de datos se realizó entre diciembre de 2020 y marzo de 2021, mediante la aplicación de un cuestionario con 40 preguntas, lo cual fue difundido en las redes sociales y aplicado a través de Formularios Google, después de previa aprobación del Comité de Ética en Investigación. Los datos se sometieron a estadística descriptiva y prueba de asociación entre variables (chi-cuadrado). Se observó que el 29,17% de los participantes desconoce sobre la disposición de los Resíduos del Servicio de Salud (RSS); el 39,58% desconoce los procedimientos de disposición de los medicamentos vencidos y sus sobras; el 47,92% desconoce el Plan de Gestión de Resíduos del Servicio de Salud (PGRSS); el 45,83% desconoce cuánto tiempo se ha implantado; y el 77,08% manifiesta falta de formación en gestión de RSS y la tiene como la principal dificultad señalada para una correcta gestión de resíduos. Es fundamental promover prácticas de educación permanente que contribuyan a la formación y el desempeño profesional en la gestión de RSS e instigar la implementación y operación del plan de gestión municipal de resíduos.

Resumo: O objetivo foi avaliar o gerenciamento de resíduos de serviços de saúde por cirurgiões-dentistas que atuam no setor público e no setor privado. O estudo foi de campo, quantitativo e descritivo, com 48 profissionais do setor público e privado. A coleta de dados ocorreu entre os meses de dezembro de 2020 e março de 2021, pela aplicação de questionário com 40 questões, o qual foi divulgado nas redes sociais e aplicado via Formulários Google, após aprovação pelo Comitê de Ética em Pesquisa. Os dados foram submetidos à estatística descritiva e ao teste de associação entre as variáveis (qui-quadrado). Observou-se que 29,17% dos participantes não têm conhecimento sobre o descarte de Resíduos de Serviço de Saúde (RSS), 39,58% desconhecem os procedimentos de descarte sobre medicamentos vencidos e suas sobras; 47,92% desconhecem o Plano de Gerenciamento de Resíduos de Serviço de Saúde (PGRSS); 45,83% desconhecem há quanto tempo este foi implementado; e 77,08% afirmaram falta de capacitação sobre gerenciamento de RSS, sendo a principal dificuldade apontada para o adequado gerenciamento de resíduos. É imprescindível fomentar práticas de educação permanente que contribuam na formação e na atuação profissional sobre gestão dos RSS e instigar a implementação e o funcionamento do plano municipal de gestão de resíduos.

ABSTRACT EnlistTM technology is a new trait on soybean and corn conferring tolerance to auxinic-type (2,4-D choline) and graminicides (aryloxyphenoxypropionates – FOPs) herbicides. However, the occurrence of volunteer corn tolerant to 2,4-D and FOPs can become a significant weed on soybean cropping systems, affecting the crop yield and reducing seed quality. This research aimed to evaluate herbicides to EnlistTM volunteer corn control and their effects on plant development, crop yield, and physiological seed quality of EnlistTM soybean. Field experiment was performed in a complete randomized block experimental design with four replicates, using a density of 10 plants·m-2 of EnlistTM volunteer corn. Treatments consisted of herbicides applied at 3-4-leaves stage of volunteer corn with haloxyfop-P-methyl, clethodim, pinoxaden, cloransulam-methyl and imazethapyr regarding to a control with and without volunteer corn. The development and yield components of EnlistTM soybean were evaluated and physiological seed testing performed on soybean’s seeds. Clethodim controlled EnlistTM volunteer corn, whereas haloxyfop-P-methyl, pinoxaden, and cloransulam-methyl showed insufficient control levels (30-35%) at 30 days after spray. Imazethapyr exhibited 84% of control of EnlistTM volunteer corn and injured the EnlistTM soybean, affecting its seed viability. EnlistTM volunteer corn interferences negatively the soybean crop yield, yield components, and affects the plant development pattern.

Abstract Introduction A. hybridus interference in soybeans varies with population size and may be quantified by mathematical models that allow establishing the threshold levels. Objective Determine the interference of A. hybridus escaped from an herbicide program and with multiple resistance to EPSPs and ALS-inhibiting herbicides on soybean yield and its economic threshold (ET). Methods Two experiments were conducted in a randomized block design. The treatments consisted of populations of A. hybridus ranging from 0 to 41 plants m-2 (2018/19) and 0 to 32 plants m-2 (2019/20). The yield components and yield plant-1 of soybean were evaluated. The rectangular hyperbola model was fitted to the soybean yield loss. ET was calculated according to yield potential, price paid for grains, control cost and weed control efficiency Results The increase in populations of A. hybridus has decreased in the number of pods and grains-1 of soybean in both periods. The parameter i was 4.47 and 8.32%, and the ET ranged from 0.16 to 1.21 plants m-2 in 2018/19 and from 0.09 to 0.65 plants m-2 in 2019/20. Conclusions One A. hybridus m-2 may reduce 6.4% in the grain yield of soybean. ET occurs within the range of 0.09 to 1.21 plants m-2.

RESUMO A tolerância ao estresse hídrico pode diferir entre espécies e cultivares. O efeito da seca nas plantas depende do impacto nos processos fisiológicos, bioquímicos e moleculares da planta e da capacidade de a mesma se adaptar a essas condições. Objetivou-se avaliar as respostas fisiológicas e bioquímicas, bem como determinar a expressão dos genes OsAPX2, OsHSP24.15, OsHSP71.10 e OsHSP85.88 em condições de seca em arroz, arroz daninho e capim-arroz. Foi realizado experimento em casa-de-vegetação, em delineamento inteiramente casualizado, com quatro repetições e dois fatores: condições hídricas (bem irrigado e déficit hídrico) e espécies de plantas [arroz (Oryza sativa cv. Puitá), arroz daninho (Oryza spp.) e capim-arroz (Echinochloa spp.)]. Em condições de seca, as plantas de arroz e arroz daninho apresentaram mais danos celulares do que o capim-arroz, e as três espécies apresentaram taxa fotossintética reduzida. As plantas C3 (arroz e arroz daninho) aumentaram os danos aos lipídios e proteínas aos 5 dias de seca. No entanto, para plantas C4 (capim-arroz), as condições de seca não afetaram os parâmetros bioquímicos. A expressão do gene OsHSP85.88 aumentou nas três plantas expostas ao déficit hídrico.

ABSTRACT The drought stress tolerance may differ among species and cultivars. The drought effect on plants depends on the impact on the plant’s physiological, biochemical and molecular processes, as well as on its ability to adapt under these conditions. This study aimed to evaluate the physiological and biochemical responses, and determine the expression of the genes OsAPX2, OsHSP24.15, OsHSP71.10 and OsHSP85.88 under drought conditions in rice, weedy rice and barnyardgrass. A greenhouse experiment was performed in a complete randomized design, with four replications and two factors: water conditions (well-watered and water deficit) and plant species [rice (Oryza sativa cv. Puitá), weedy rice (Oryza spp.) and barnyardgrass (Echinochloa spp.)]. Under drought conditions, the rice and weedy rice plants showed more cellular damage than the barnyardgrass, and the three species showed a reduced photosynthetic rate. C3 plants (rice and weedy rice) increased the damage to lipids and proteins at 5 days of drought. However, for C4 plants (barnyardgrass), the drought conditions did not affect the biochemical parameters. The expression of the OsHSP85.88 gene increased in the three plants exposed to water deficit.

Abstract The objective is to verify the effects of pesticides on the health of farmers in the southern region of Brazil, as well as the possible symptoms and cases of intoxication. This study has a quantitative, descriptive and exploratory approach, carried out in 12 agricultural locations in the municipality of Serra Catarinense. This research was approved by the research ethics committee and was applied through a structured questionnaire to 79 farmers who grow grains in the conventional cultivation system. The pesticide most used by farmers was glyphosate, followed by acephate. Of the individuals, 21.5% reported that they had suffered poisoning by pesticides by the respiratory route (58.8%). In addition, 28% had three symptoms of intoxication, demonstrating possible probable cases of intoxication. And 41% of workers reported headaches as a predominant symptom in applications with the use of pesticides, followed by dizziness/vertigo (16%). Farmers who use PPE during the preparation of the pesticide spray are neither chronic diseases (p<0.003) nor psychological problems (p<0.000). All four individuals who had cancer, all also eat while applying pesticides (p<0.049). The exposure to pesticides causes changes in the organism of those exposed, using them more vulnerable to health problems.

Resumo - O objetivo deste trabalho foi avaliar aspectos ecofisiológicos da germinação de sementes em biótipos de sagitária (Sagittaria montevidensis) resistentes e suscetível a herbicidas. O delineamento experimental foi o inteiramente casualizado. Em campos de arroz, foram colhidas sementes de dois biótipos resistentes (SAGMO 10 e SAGMO 32) e de um suscetível (SAGMO 35) aos herbicidas inibidores da acetolactato sintase e do fotossistema II. A liberação da dormência foi realizada com 2,0% de nitrato de potássio, escarificação química (1,0% de H2SO4 por 30 s) e mecânica (lixa), 10 ppm de ácido giberélico, embebição em água por 24 horas, banho-maria a 60ºC e um controle. Avaliaram-se a germinação às temperaturas de 10 a 40°C e a emergência de plântulas aos 0,0, 0,5, 1,0, 2,0, 4,0 e 5,0 cm de profundidade de enterrio. Todos os biótipos apresentaram germinação superior a 70% após escarificação mecânica. A maior taxa de germinação ocorre a 25,9°C para SAGMO 35, o biótipo suscetível, e a 26,2 e 26,5°C, respectivamente, para SAGMO 10 e SAGMO 32, biótipos resistentes. Para todos os biótipos, a maior emergência de plântulas ocorre em sementes posicionadas a 0,5 e 1,0 cm de profundidade de enterrio.

Abstract - The objective of this work was to evaluate the ecophysiological aspects of seed germination in California arrowhead (Sagittaria montevidensis) biotypes resistant and susceptible to herbicides. The experimental design was completely randomized. In paddy rice fields, seeds were collected from two biotypes that are resistant (SAGMO 10 and SAGMO 32) and from one that is susceptible (SAGMO 35) to acetolactate synthase and photosystem II inhibiting herbicides. Seed dormancy release was performed with 2.0% potassium nitrate, chemical (1.0% H2SO4 for 30 s) and mechanical (sandpaper) scarification, 10 ppm gibberellic acid, water imbibition for 24 hours, water bath at 60°C, and a control. Germination was evaluated at temperatures from 10 to 40°C and seedling emergence at 0.0, 0.5, 1.0, 2.0, 4.0, and 5.0 cm burial depths. All biotypes showed a germination higher than 70% after mechanical scarification. The highest germination rate occurs at 25.9°C for SAGMO 35, the susceptible biotype, and at 26.2 and 26.5°C, respectively, for SAGMO 10 and SAGMO 32, the resistant biotypes. For all biotypes, the highest seedling emergence occurs in seeds positioned at the 0.5 and 1.0 cm burial depths.

Resumo Introdução: O uso de agrotóxicos pode desencadear doença renal. Objetivo: Descrever a exposição a agrotóxicos de pacientes com doença renal crônica em tratamento dialítico. Métodos: Pesquisa de campo, quantitativa e descritiva, com 90 portadores de doença renal crônica em tratamento dialítico em duas unidades de hemodiálise no estado de Santa Catarina, por meio da aplicação de um questionário estruturado. Os participantes foram divididos em dois grupos: sem e com exposição a agrotóxicos. O questionário foi aplicado nas clínicas de hemodiálise durante o tratamento. Foram coletados valores de exames laboratoriais dos prontuários clínicos. Os dados foram analisados pela estatística descritiva e associação pelo teste qui-quadrado. Para os dados dos exames laboratoriais, foi realizada comparação de médias pelo teste t de Student não pareado entre os grupos. Resultados: A idade média dos participantes expostos foi de 58 anos (±13,7; mínimo = 23; máximo = 75) e a dos não expostos, de 64 anos (±13,9; mínimo = 35; máximo = 96). Dos 90 pacientes, 30% foram expostos a agrotóxicos. O tempo médio de exposição foi de 6,7 ± 3,8 horas/dia. Houve associação estatística significativa entre o preparo da calda com agrotóxicos e a presença de diabetes (p ≤ 0,048). Não houve diferença estatística significativa entre os resultados dos exames laboratoriais do grupo exposto e do não exposto. Conclusão Esta pesquisa evidencia que os agrotóxicos podem ser fatores desencadeadores da doença renal crônica (DRC), entretanto sugere-se ampliar pesquisas na área que possam comprovar a relação entre exposição a agrotóxicos e DRC.

ABSTRACT Introduction: Pesticides can trigger kidney disease. Objective: To describe the exposure to pesticides of patients with chronic kidney disease on dialysis. Methods: Quantitative and descriptive field research, with 90 patients with chronic kidney disease on dialysis in two hemodialysis units in the state of Santa Catarina, through the application of a structured questionnaire. Participants were divided into two groups: with and without exposure to pesticides. The questionnaire was applied in hemodialysis clinics during treatment. Laboratory test values were collected from clinical records. Data were analyzed using descriptive statistics and association using the chi-square test. For laboratory test data, a comparison of means was performed using the unpaired Student’s t-test between the groups. Results: The mean age of exposed participants was 58 years (±13.7; minimum = 23; maximum = 75) and that of non-exposed participants was 64 years old (±13.9; minimum = 35; maximum = 96). Of the 90 patients, 30% were exposed to pesticides. The mean exposure time was 6.7 ± 3.8 hours/day. There was a statistically significant association between the preparation of the mixture with pesticides and diabetes (p ≤ 0.048). There was no statistically significant difference between the results of laboratory tests in the exposed and non-exposed groups. Conclusion: This study shows that pesticides can be triggering factors for chronic kidney disease (CKD); however, we must expand research in this field to prove the relationship between exposure to pesticides and CKD.

Abstract: Background: Ryegrass is considered an important weed in crops in the state of Rio Grande do Sul, Brazil, for being resistant to the herbicide glyphosate. Objective: This study evaluates ryegrass control by applying different spray volumes and doses of clethodim alone or in combination with glyphosate and/or 2,4-D. Methods: Two field experiments were conducted in a 5 x 3 factorial design. In experiment I, factor A consisted of clethodim (96 g a.i. ha-1); clethodim (96 g a.i. ha-1) + glyphosate (1,080 g a.e. ha-1); clethodim (96 g a.i. ha-1) + 2,4-D (1,047 g a.e. ha-1); clethodim (96 g a.i. ha-1) + glyphosate (1,080 g a.e. ha-1) + 2,4-D (1,047 g a.e. ha-1); and control without treatment. Factor B consisted of three spray volumes (40, 80, and 120 L ha-1). In the second experiment, factor A consisted of the same herbicides and their associations aforementioned and factor B consisted of three increasing doses of the herbicide clethodim (96, 192 and 288 g a.i. ha-1). The herbicides were applied at the milky stage of ryegrass grains. Ryegrass control at 10, 20, and 30 days after application (DAA) of herbicides and dry matter were evaluated. Results: Spray volume reduction did not interfere with ryegrass control by clethodim and glyphosate or clethodim, glyphosate, and 2,4-D at 30 DAA, but it did interfere with the application of clethodim alone or associated only with 2,4-D. Doses above 192 g a.i. ha-1 of clethodim alone or in mixture were efficient for ryegrass control. Conclusions: Greater clethodim doses and spray volume increased control of ryegrass at the milky grain stage and decreased the antagonism in the associations with 2,4-D.

RESUMO As plantas sintetizam metabólitos especializados para defenderem-se contra patógenos. Estes compostos ativos, quando isolados e identificados, podem ser utilizados como modelos para o desenvolvimento de fungicidas. Baseado em observações a campo, as plantas de Heteranthera reniformis (aguapé-mirim) são promissoras na síntese de compostos com atividade antifúngica. O objetivo deste estudo foi avaliar a atividade antifúngica de extratos de H. reniformis sobre o desenvolvimento de Bipolaris oryzae. A atividade dos extratos hexano, acetato de etila e metanol de folhas de H. reniformis foi avaliada no crescimento micelial e esporulação de B. oryzae. Devido a maior inibição da esporulação de B. oryzae, o extrato metanólico foi escolhido para o experimento de concentrações. O efeito do extrato metanólico sobre a morfologia das colônias, comprimento e germinação dos esporos de B. oryzae também foi avaliado. Os resultados demonstraram que o extrato metanólico foi o mais ativo na inibição da esporulação de B. oryzae. A morfologia das colônias é alterada quando o fungo cresce em meio contendo extratos foliares de H. reniformis. As maiores concentrações do extrato metanol estimulam o crescimento micelial e suprimem a esporulação de B. oryzae. Há reduções no comprimento e germinação de esporos de B. oryzae pelo extrato metanólico de folhas de H. reniformis. O extrato metanólico tem compostos com atividade antifúngica e deve ser submetido ao isolamento guiado por bioensaio para a purificação e identificação destes compostos.

ABSTRACT Plants synthesize specialized metabolites to defend themselves against pathogens. These active compounds, when isolated and identified, can be used as template for fungicide development. Based on field observations, Heteranthera reniformis (kidney leaf mudplantain) could potentially synthesize compounds with antifungal activity. The goal of this study was to evaluate the fungicidal activity of H. reniformis leaf extracts on Bipolaris oryzae development. The activities of hexane, ethyl acetate, and methanol extracts of H. reniformis leaves were tested on mycelial growth, sporulation, and colony morphology. Due to the highest inhibition of B. oryzae sporulation, methanol extract was chosen for concentration tests. The effect of methanol extract on B. oryzae spore length and germination was also evaluated. Methanolic extract was the most active in inhibiting B. oryzae sporulation. The morphology of the colonies is altered when the fungus grows in medium containing H. reniformis leaf extracts. Higher concentration of methanol extract stimulates mycelial growth and suppresses B. oryzae sporulation. There are reductions in length and number of germinated B. oryzae spores caused by methanol extract of H. reniformis leaves. Methanolic extract has compounds with antifungal activity and should be subjected to bioassay-guided isolation for purification and identification of these active compounds.

Abstract Background Weed competition is one of the main constraints to rice yield. Objective Determine the period prior to interference (PPI) of barnyardgrass ( Echinochloa spp.) on the irrigated rice crop as a function of the application of the herbicide cyhalofop-butyl alone and associated with penoxsulam. Methods A field experiment was carried out in a randomized block design, with four replications. The treatments were arranged in a 2×7 factorial scheme, with factor A consisting of the herbicides cyhalofop-butyl and the mixture penoxsulam + cyhalofop-butyl, while factor B consisted of seven coexistence periods: 0, 7, 14, 21, 28, 35, and 120 days after rice emergence (DAE). The herbicides were applied in each of these periods. The analyzed variables were crop height and shoot dry mass of the rice and barnyardgrass, number of grains per panicle, thousand-grain weight, and yield. Results The presence of barnyardgrass competing for environmental resources negatively affected the analyzed variables and rice yield for all periods with a coexistence longer than 28 DAE. Barnyardgrass competition during the entire rice cycle (120 DAE) reduced the shoot dry mass of the crop. Conclusions The PPI termination was determined at 14 DAE for the mixture cyhalofop-butyl + penoxsulam and 29 DAE for cyhalofop-butyl alone.

ABSTRACT The aim of the present study was to assess the relative competition between white clover (cv. BRS URS Entrevero) and weed species Silene gallica L. through replacement series. The experiments followed a completely randomized design with four replications. The first study showed that the shoot dry mass of both species (white clover and S. gallica) became constant with 16 plants.pot-1 or 400 plants.m-2. In 2017, a study was conducted and it was repeated in 2018 in order to assess white clover competition with S. gallica under different crop: weed proportions pot-1 (100:00; 75:25; 50:50; 25:75 and 00:100). Competitiveness analysis was carried out through diagrams applied to replace experiments and through the relative competition indices. Morphological parameters such as plant height, leaf area and shoot dry mass of the crop and the weed were measured when S. gallica plants bloomed (105 days after species emergence). Results have proven the phenotypic plasticity of temperate-climate foragers such as white clover. This outcome shows that the competitive relationship with Silene gallica changes due to plant proportions composing the association. Therefore, it was possible to conclude that the species show similar competitive ability.

ABSTRACT: Italian ryegrass (Lolium multiflorum Lam.) is a weed broadly found in fields cultivated with wheat and barley crops. Seed inputs into the soil before harvesting winter crops increase seed bank, ensuring survival. This study evaluated the persistence of Italian ryegrass seeds subjected to burial depths over time. Experiments were carried out in the field for two years in randomized block experimental design with four repetitions. Dormant seeds harvested from mother-plants were put in nylon bags containing soil. Previously, seed viability was evaluated in a 1 % tetrazolium solution, and 50 viable seeds by repetition were buried at 0.5, 5.0, 10, and 20 cm depths. Seed persistence was evaluated by the percentage of deteriorated and remaining seeds, non-dormant seeds, abnormal seedlings, and viable and non-viable dormant seeds at 0, 30, 60, 90, 180, 360, 540, and 720 days after burial (DAB). Seed persistence increased at 10 and 20 cm of burial depth compared to seeds in the soil layers up to 5 cm. Moreover, burial depth at 10 and 20 cm showed lower percentage of deteriorated seeds (10 % lower) compared to 0.5 cm at 360 DAB. For non-dormant seeds, a higher percentage was found at 90 DAB, regardless of seed burial depth. Dormancy breakage occurred until 180 DAB, and more rapidly at 10 and 20 cm depths. At 540 DAB, more than 95 % of seeds were unviable, demonstrating short persistence of Italian ryegrass in the soil seed bank.