ABSTRACT The Sonoran semi-desert with its stressful conditions of high temperature, salinity and drought affect the plant physiological performance and productivity. In this region, a study was carried out with the aim of evaluating the dynamics of leaf development, NDVI (Normalized Difference Vegetation Index), leaf senescence time and primary productivity of Parkinsonia aculeata L. Sp. Pl. in three sites there where high temperatures conditions prevail. It was found that heat does not affect the development dynamics nor leaves length, however, drought does, reducing their development rate to 0.2 cm day-1. The NDVI was more affected by drought condition, with values lower than 0.79. Salinity caused leaves senescence at 67 days. Parkinsonia aculeata L. Sp. Pl. presented higher primary productivity under heat condition, generating 116 g m-2 month-1.

RESUMEN El semidesierto de Sonora con sus condiciones estresantes de altas temperaturas, salinidad y sequía afectan el desempeño fisiológico y la productividad de las plantas. En esta región se realizó un estudio con el objetivo de evaluar la dinámica del desarrollo de las hojas, el NDVI (Índice de Vegetación de Diferencia Normalizada), el tiempo de senescencia de las hojas y la productividad primaria de Parkinsonia aculeata L. Sp. Pl. en tres sitios donde prevalecen condiciones de altas temperaturas, salinidad y sequía. Se encontró que las altas temperaturas no afectan la dinámica del desarrollo ni la longitud de las hojas, sin embargo, la sequía reduce sí, reduciendo su ritmo de desarrollo a 0.2 cm día-1. La condición de sequía fue la que más afectó el NDVI se afecta más por la condición de sequía, con valores inferiores al 0.79. La salinidad causó la senescencia de las hojas a los 67 días. Parkinsonia aculeata L. Sp. Pl. presentó mayor productividad primaria en la condición de calor, generando 116 g m-2 mes-1.

RESUMO O objetivo no trabalho foi avaliar o efeito de dessecantes químicos e épocas de colheita sobre a qualidade fisiológica e expressão enzimática em sementes de soja. O delineamento experimental foi blocos casualizados, com quatro repetições, em esquema fatorial (4 × 3 + 1): quatro dessecantes (paraquat - 2 L ha-1, glufosinato de amônio - 2 L ha-1, diquat - 1,5 L ha-1, saflufenacil 40 g ha-1) aplicado no estádio fenológico R7.1 (início do amarelecimento das folhas), com três épocas de colheita (0; 14 e 28 dias após o estádio fenológico R8) mais o controle (ausência de dessecante e colhida em R8). Foi avaliada a qualidade fisiológica das sementes de soja por meio de testes em laboratório e a expressão enzimática das enzimas malato desidrogenase, álcool desidrogenase, esterase, isocitrato liase e para a superóxido dismutase. A colheita de sementes no estádio R8+14 promove as maiores perdas na qualidade das sementes, todavia, não só tempo de atraso afeta, mas a ocorrência de precipitações é decisiva, sendo que chuva de 50 mm, já proporciona deterioração das sementes de soja. Os dessecantes diquat e paraquat foram os que promoveram os menores e maiores danos à qualidade fisiológica das sementes, respectivamente. A expressão das enzimas álcool desidrogenase, esterase e isocitrato liase são eficientes no monitoramento e com adequada correlação com a qualidade fisiológica. A malato desidrogenase e superóxido dismutase apresentam baixa correlação com os testes fisiológicos realizados nas sementes de soja.

ABSTRACT This study aimed to evaluate the effect of chemical desiccants and harvest times on the enzymatic expression and physiological quality of soybean seeds. The experiment was carried out in a randomized block design with four replications, in a factorial scheme (4 × 3 + 1) with four desiccants (paraquat - 2 L ha-1, ammonium glufosinate - 2 L ha-1, diquat - 1.5 L ha-1, and saflufenacil - 40 g ha-1). These were applied at the phenological stage R7.1 (beginning of leaf yellowing) and at three harvest times (0, 14, and 28 days after the phenological stage R8). There was also a control treatment (no desiccant, harvested at R8). The physiological quality of soybean seeds and the enzymatic expressions of malate dehydrogenase, alcohol dehydrogenase, esterase, isocitrate lyase, and superoxide dismutase were evaluated in laboratory tests. Seeds harvested at the R8 + 14 stage led to the highest losses in seed quality. However, the 50 mm rainfall also affected seed deterioration. The desiccants diquat and paraquat provided the lowest and the highest damage to the seed physiological quality, respectively. The expression of the enzymes alcohol dehydrogenase, esterase, and isocitrate lyase were efficient and had an adequate correlation with the physiological quality. Malate dehydrogenase and superoxide dismutase had no satisfactory relation with the physiological tests performed with soybean seeds.

RESUMO O uso de dessecantes pode permitir a obtenção de sementes com máxima qualidade fisiológica e sanitária, devido o menor período em que as sementes ficam expostas as condições adversas no campo aguardando o estádio de maturação para colheita. O objetivo neste trabalho foi avaliar o efeito de dessecantes químicos e épocas de colheita sobre a qualidade fisiológica e sanitária em sementes de soja. O ensaio foi realizado em delineamento em blocos casualizados, disposto em esquema fatorial (4 × 3) + 1, sendo quatro herbicidas para a dessecação (gramoxone - 2 L ha-1, glufosinato - 2 L ha-1, reglone - 1,5 L ha-1, saflufenacil 40 g ha-1) aplicado no estádio fenológico R7.1 (maturidade fisiológica e 65% de umidade), com três épocas de colheita (0; sete e 14 dias após o estádio fenológico R8 - maturação plena), mais o controle (ausência de dessecante e colhida em R8), com quatro repetições. O uso do dessecante gramoxone e as sementes colhidas sete dias após R8 proporciona maior qualidade fisiológica de sementes de soja, conforme observado nas variáveis germinação, danos por umidade, danos mecânicos e a incidência de Colletotrichum sp., Phomopsis sp., Penicilium sp., Fusarium sp., Cercospora kikuchii e Alternaria sp.. A colheita de sementes no estádio R8+14 promove as maiores perdas na qualidade das sementes. O uso de glufosinato de amônio e saflufenacil como dessecantes não são recomendados, devido à redução na qualidade fisiológica das sementes de soja.

ABSTRACT The use of desiccants may result in seeds with high physiological and sanitary quality due to the shorter period of exposure to field adverse conditions before the maturity stage for harvest. This study evaluated the effect of chemical desiccants and harvest times on the physiological and sanitary quality of soybean seeds. The experiment consisted of a randomized block design, arranged in a factorial scheme (4 × 3) + 1, with four replications. The first factor corresponded to four desiccation (grammoxone-2 L ha-1, glufosinate-2 L ha-1, reglone-1.5 L ha-1, and saflufenacil-40 g ha-1) applied at the R7.1 phenological stage (physiological maturity and 65% moisture with three harvest times (0, 7, and 14 days after the R8 phenological stage) and additional treatment (control, application of water only, and harvest at the R8 stage), with four replications. The use of the grammoxone desiccant and seeds harvested at seven days after the R8 stage resulted in soybean seeds of the highest physiological quality, as observed for the variables germination, moisture damage, mechanical damage, and incidences of Colletotrichum sp., Phomopsis sp., Penicillium sp. Fusarium sp., Cercospora kikuchii, and Alternaria sp. Seeds harvested at the R8 + 14 stage had the greatest losses in seed quality. The use of ammonium glufosinate and saflufenacil as desiccants is not recommended due to the reduction in the physiological quality of soybean seeds.

In this study the toxicological evaluation at repeated dose by the method of limit test of the candidate to magnetically treated immunopotentiator CM-95 Solution was carried out; according with regulations of the Organization for Economic Cooperation and Development included in guide 407. The objective was to establish the possible organic and functional lesions caused by the magnetically treated CM-95 Solution with maximum permissible magnetic induction (0,16 T), for obtaining the candidate to immunopotentiator. Three groups were used: Experimental, Control and Satellite. As experimental Biomodels, male and female Sprague Dawley rats were used, with 150 to 200 g weights. During the study neither clinical signs of toxicity nor deaths in any of the animals of the treated groups were registered. There was not affectation of the corporal weight during the test. There was not change of the corporal weight during the trial. Although there were variations of some haematology and biochemical parameters, they had no biological significance. Macroscopic or microscopic lesions were not found; just proliferation effects were observed, in the lymphoid tissues of the thymus and spleen, related to the response of immune system. Magnetically treated CM-95 Solution, showed no toxicity in the animal model and dose level used, and under the observed experimental conditions.

En este trabajo se realizó la evaluación toxicológica a dosis repetidas por el método de test límite del candidato a inmunopotenciador, la solución CM-95 tratada magnéticamente, acorde con las regulaciones de la Organización para la Colaboración Económica y el Desarrollo, incluida en la Guía 407. El objetivo fue establecer las posibles lesiones orgánicas y funcionales ocasionadas por la solución CM-95, tratada magnéticamente con la máxima inducción magnética permisible (0,16 T), para la obtención del candidato a inmunopotenciador. Se emplearon tres grupos: Experimental, Control y Satélite. Como Biomodelo experimental se utilizaron ratas Sprague Dawley machos y hembras con pesos de 150 a 200 g. Durante el estudio no se registraron signos clínicos de toxicidad ni muertes en ninguno de los animales de los grupos tratados, ni en los controles. No hubo afectación del peso corporal durante el ensayo. Aunque hubo variaciones en los valores de algunos parámetros hematológicos y bioquímicos, estos no tuvieron significación biológica. No se encontraron lesiones macroscópicas, ni microscópicas; solo se observaron efectos proliferativos en el tejido linfoide de timo y bazo, relacionados con la respuesta del sistema inmune. La solución CM-95 tratada magnéticamente, no mostró toxicidad en el modelo animal y nivel de dosis utilizado, y bajo las condiciones experimentales ensayadas.

En comparación con el tratamiento convencional, la terapia endovascular en aneurisma de aorta torácica, presenta los mejores resultados, por lo que se convierte en el tratamiento de elección para la patología de aorta torácica descendente endovascular, por su baja morbimortalidad perioperatoria. El tratamiento quirúrgico por vía retroperitoneal y/o endovascular para aneurisma de aorta abdominal infrarrenal, resulta ser especialmente seguro en pacientes octogenarios o con alta morbilidad. Esta cohorte institucional presenta resultados perioperatorios y en el seguimiento, similares a los reportados en la literatura mundial.

Compared with the conventional treatment, endovascular therapy in thoracic aortic aneurysm shows the best results, being the election treatment for the pathology of the descending thoracic aorta, due to its low peri-operative morbid-mortality. Surgical treatment by retro-peritoneal route and/or endovascular for infra-renal abdominal aortic aneurysm is especially safe in octogenarian patients or in those with a high mortality rate. This institutional cohort show peri-operative and follow-up results similar to those reported in the world literature.

En estos momentos es posible hacer recomendaciones sobre las indicaciones de la endarterectomía carotídea, basadas en los ensayos clínicos publicados y en la revisión de grandes series quirúrgicas. Las indicaciones para la cirugía dependerán del grado de estenosis y de la morfología de la placa, así como del estado clínico del paciente y de la morbi-mortalidad del equipo quirúrgico. Este al igual que otros estudios con grandes series de casos, evidencia que la endarterectomía carotídea y/o el manejo endovascular, realizado por equipos expertos, es un procedimiento seguro en pacientes con indicación quirúrgica.

It is now possible to recommend indications for aortic endarterectomy, based on published clinical essays and revision of large surgical series. Surgery indications will depend on the stenosis degree and the plaque morphology, as well as on the patient's clinical state and the morbid-mortality in this surgical team. As other studies with large case series, evidences that carotid endarterectomy and/or endovascular management, when realized by expert teams, is a safe procedure in patients with surgical indication.