Resumo: O Parque Estadual de Porto Ferreira (PEPF) está localizado no estado de São Paulo, sudeste do Brasil, em uma intrigante área de contato entre a Mata Atlântica e o Cerrado - ambos hotspots de biodiversidade -, representada principalmente pelo cerradão (CER), a floresta estacional semidecidual (FES) e pela variação dessa última, com influência aluvial (FESA). As áreas ecotonais desempenham um papel importante no conhecimento ecológico e fitogeográfico da flora e da vegetação. Mesmo com muitos estudos realizados no PEPF, as informações encontram-se dispersas. Dessa forma, produzimos um checklist atualizado da flora vascular, resultante da compilação de vouchers, estudos realizados no PEPF e de esforços de coleta realizados pelos autores entre os anos de 2014 e 2017. Além disso, para entender suas relações fitogeográficas, realizamos uma análise de similaridade florística entre o PEPF e outros levantamentos realizadas nas mesmas fitofisionomias da área estudada. Registramos 684 espécies pertencentes a 387 gêneros e 107 famílias. A FES foi a fitofisionomia mais rica (478 espécies), seguida pelo CER (418) e a FESA (231). As famílias mais ricas foram Fabaceae (64 espécies), Myrtaceae (41), Orchidaceae (39), Rubiaceae (37), Asteraceae (35), Bignoniaceae (26) e Malvaceae (20). Oito espécies ameaçadas em âmbito regional e nacional foram encontradas. Desde a última lista florística produzida para o PEPF, 412 espécies foram adicionadas. As formas de vida com maiores números de espécies foram: árvores (286 espécies), ervas (176) e arbustos mais subarbustos (123). A análise de similaridade corroborou o padrão de que áreas mais próximas geograficamente são mais similares entre si, e esse padrão foi encontrado, também, para áreas ecotonais envolvendo diferentes fitofisionomias. Os resultados encontrados colocam o PEPF entre as unidades de conservação com maior número de espécies vegetais sob o domínio de clima sazonal, possuindo, portanto, grande importância para a conservação de espécies de plantas no Sudeste do Brasil.

Abstract: The Porto Ferreira State Park (PFSP) is located in the State of São Paulo southeastern Brazil, in an intriguing transitional area between the Atlantic Forest and Cerrado - both hotspots of biodiversity - represented mainly by the cerradão (CER), and the seasonal semideciduous forest (SSF), with its alluvial variation vegetation type (riparian forest - RP). Ecotonal areas play an important role in providing ecological and phytogeographic knowledge regarding the flora and vegetation of this region. Despite various studies on the PFSP, knowledge of this region remains fragmented. In this study, we aim to conduct an updated checklist of the PFSP vascular flora, including a compilation of all the studies conducted in this protected area, plus field work carried out by the authors from 2014 to 2017. In addition, given its ecotonal characteristics, we completed a floristic similarity analysis between the PFSP and other floristic surveys that examined the same vegetation types present in this study, to gain a better understanding of their phytogeographic relationships. Overall, 684 species, belonging to 387 genera and 107 families, were recorded. The SSF presented the richest vegetation type (478 species), followed by the CER (418) and the RP (231). The most diverse families were Fabaceae (64 species), Myrtaceae (41), Orchidaceae (39), Rubiaceae (37), Asteraceae (35), Bignoniaceae (26) and Malvaceae (20). Moreover, eight threatened species, at regional and national levels, were found. To date, 412 species have been added to the floristic list produced for the PFSP. The life forms with the highest number of species were trees (286 species), herbs (176) plus shrubs and subshrubs (123). Our research findings indicate floristic patterns with higher levels of similarity among species in geographical proximity, including those in ecotonal areas encompassing different vegetation types. These results rank the PFSP among some of the most species-rich conservation units with seasonal climates, and therefore is of great importance for plant conservation in the southeast of Brazil.

ABSTRACT Objective: To build a model to evaluate the impact of salvage radiotherapy (SRT) in men with PSA rise or persistent PSA after undergoing radical prostatectomy (RP). Materials and Methods: The study included 107 node-negative patients treated with SRT after RP at a single institution. Patients received SRT for either prostate-specific antigen (PSA) rising, or PSA persistence after RP. All patients received local radiation to the prostate / seminal vesicle bed. The primary measured outcome was the biochemical recurrence (BCR) free survival. Multivariable Cox regression analysis was used to develop a risk-stratification group to identify predictive factors associated with the probability of BCR at 5yr. Results: At a median follow-up of 52 months, the BCR free survival rate and overall survival in 5 years was 73% and 94%, respectively. At multivariable analysis, pre-SRT PSA level > 0.35ng / mL (p = 0.023), negative margins (p = 0.038), and seminal vesicles invasion (p = 0.001) were significantly associated with BCR free survival. Three risk groups using regression analysis for SRT administration was built. Low-, intermediate- and the high-risk groups had a BCR free survival in 5-years of 96%, 84%, and 44% (p = 0.0001), respectively. Conclusions: We developed a risk group stratification to show the impact of SRT based on prostate cancer characteristics. SRT showed to be extremely beneficial for patients with low- and intermediate-risk tumors. Moreover, the risk-group built could identify patients classified as high-risk who might benefit from more aggressive treatment for SRT.

Resumo O objetivo deste estudo foi utilizar a polpa dos frutos do Cerrado brasileiro araticum e cagaita para desenvolver novos produtos e avaliar a aceitabilidade sensorial por crianças de 7 a 9 anos de idade. Os produtos desenvolvidos foram: três formulações de doce de leite: DP (padrão), DA (com polpa de araticum) e DB (com polpa de araticum e coco ralado); três formulações de refresco de cagaita: RP (polpa de cagaita), RA (polpa de cagaita e suco de laranja) e RB (polpa de cagaita e suco de cenoura); e uma formulação de geleia de cagaita. Verificou-se que as três formulações de doce de leite foram igualmente aceitas (p>0,05), enquanto o refresco RA foi mais aceito (p<0,05) que os demais (RP e RB). Além disso, a geleia de cagaita apresentou elevados escores de aceitação sensorial. Todos os produtos desenvolvidos foram bem aceitos, uma vez que seus índices de aceitabilidade foram maiores que 70%. Não houve influência (p>0,05) do gênero sobre a aceitabilidade sensorial dos produtos avaliados. Conclui-se que os produtos desenvolvidos apresentaram boa aceitabilidade do ponto de vista sensorial, o que sugere um elevado potencial de mercado.

Summary The objective of this study was to use the pulp of the Brazilian Savannah fruits araticum and cagaita to develop new products, and to evaluate their sensory acceptance by children from 7 to 9 years of age. The products developed were: three milk caramel formulations: DP (standard), DA (araticum pulp) and DB (araticum pulp and grated coconut); three cagaita refresher formulations: RP (cagaita pulp), RA (cagaita pulp and orange juice) and RB (cagaita pulp and carrot juice); and a cagaita jam formulation. It was found that the three formulations of milk caramels were equally accepted (p>0.05), whereas the RA refresher was more accepted (p<0.05) than the others (RP and RB). In addition, the cagaita jam received high sensory acceptance scores. All the products developed were well accepted since their acceptability indexes were greater than 70%. There was no influence (p>0.05) of gender on the sensory acceptability of the products evaluated. It was concluded that the products developed showed good acceptability from a sensory point of view, which suggests high market potential.

O experimento foi conduzido para comparar a eficiência dos métodos do disco medidor de forragem (DMF), o bastão graduado (BG), a régua e o medidor de capacitância (MC), utilizados em estimativas da massa de forragem (MF) em pastagens. Os instrumentos foram empregados em pastagens de capim-estrela (Cynodon nlemfuensis Vanderyst var. nlemfuensis) usadas sob condições de pastejo contínuo, taxa de lotação variável e em diferentes alturas do pasto. O delineamento foi o inteiramente ao acaso com duas repetições e os tratamentos foram quatro alturas do pasto: 5, 10, 15 e 20cm. A altura do pasto nos piquetes foi mantida por ajustes da taxa de lotação, usando-se novilhas da raça Nelore (Bos indicus). As avaliações nos pastos foram realizadas em períodos mensais, de 7/11/2007 a 26/4/2008. A efetividade de cada método para quantificar a MF foi avaliada por equação de regressão. O MC não apresentou resultados consistentes. Os valores obtidos de coeficiente de determinação para avaliação da MF foram baixos e inferiores a 0,63 com o BG, DMF e com a régua. Medições de MF em pastos de capim-estrela sob pastejo contínuo podem ser realizadas preferivelmente com o DMF, ou com o BG e a régua.

The experiment was conducted aiming to compare the efficiency of the methods rising plate meter (RP), sward stick (SS), ruler and capacitance meter (CM), used to estimate herbage mass on pastures. The instruments were used in stargrass pasture (Cynodon nlemfuensis Vanderyst var. nlemfuensis) under continuous stocking, variable stocking rate, and sward heights. The design was completely randomized with two replicates and the treatments were sward heights: 5, 10, 15, and 20cm. The sward height at paddock was maintained by stocking rate adjustments using Nellore heifers (Bos indicus). The swards were evaluated monthly, from November 7th, 2007 to April 26th, 2008. The effectiveness of each method to quantify the herbage mass was evaluated by the regression equation. The capacitance meter did not show consistent results. The values of coefficient of determination for the evaluation of herbage mass were low and less than 0.63 with the SS, RP and the ruler. Measurements of herbage mass on swards of stargrass under continuous stocking can be performed preferably with the RP, or with the SS and the ruler.

This experiment was designed with the objective of developing a simple, practical, and high repeatability technique for the simultaneous evaluation of the integrity of the plasmatic and acrosomal membranes, as well as funcional mitochondria of domestic fowl spermatozoa using an association of fluorescent probes. Four ejaculates (motility > 80% and abnormal morphology < 10%) from each of six Ross male broiler breeder (n=24) were diluted in TALP sperm medium (25x10(6) spermatozoa/mL) and split into two aliquots, and one of these aliquots was flash frozen in liquid nitrogen and thawed to damage all cellular membranes. Three treatments were prepared from these aliquots, with the following ratios of Fresh semen:Flash frozen semen: 100:0 (T100), 50:50 (T50), and 0:100 (T0). A 150-µL aliquot of diluted semen was placed in a microcentrifuge tube with the addition of 2-µL PI, 2-µL MITO, and 50-µL FITC-PSA, and incubated at 38.5º C/8 min in the dark. An 8-µL sample was placed on a slide, coverslipped, and examined by epifluorescence microscopy. Each sample had 200 cells counted and classified based on the fluorescence emitted by each probe. By regression analysis, plasma membrane integrity, as detected by PI, was determined as: v=4.17+0.82X (R²=0.95). Acrosome integrity, as detected by FITC-PSA, generated the equation: v=4.19+0.84X (R²=0.96). Functional mitochondria was estimated by the equation v=3.20+0.83X (R²=0.96). This is an efficient technique to simultaneously evaluate plasmatic, acrosomal, and mitochondrial membranes in fowl sperm. It is suggested that its application in flow cytometry systems allows this methodology to be applied in large scale.