A gastroenteritis outbreak that occurred in 2013 in a low-income community in Rio de Janeiro was investigated for the presence of enteric viruses, including species A rotavirus (RVA), norovirus (NoV), astrovirus (HAstV), bocavirus (HBoV), aichivirus (AiV), and adenovirus (HAdV). Five of nine stool samples (83%) from patients were positive for HAdV, and no other enteric viruses were detected. Polymerase chain reaction products were sequenced and subjected to phylogenetic analysis, which revealed four strains and one strain of non-enteric HAdV-A12 and HAdV-F41, respectively. The HAdV-A12 nucleotide sequences shared 100% nucleotide similarity. Viral load was assessed using a TaqMan real-time PCR assay. Stool samples that were positive for HAdV-A12 had high viral loads (mean 1.9 X 107 DNA copies/g stool). All four patients with HAdV-A12 were < 25 months of age and had symptoms of fever and diarrhoea. Evaluation of enteric virus outbreaks allows the characterisation of novel or unique diarrhoea-associated viruses in regions where RVA vaccination is routinely performed.

RESUMO OBJETIVO Determinar o número necessário de leitos públicos de unidades de terapia intensiva para adultos no estado do Rio de Janeiro para atender à demanda existente, e comparar os resultados com a recomendação do Ministério da Saúde. MÉTODOS Seguiu-se modelo híbrido que agrega séries temporais e teoria de filas para prever a demanda e estimar o número de leitos necessários. Foram considerados quatro cenários de fluxo de pacientes, de acordo com as solicitações de vagas, proporção de desistências e tempo médio de permanência no leito de unidade de terapia intensiva. Os resultados foram confrontados com os parâmetros do Ministério da Saúde. Os dados foram obtidos da Central Estadual de Regulação, de 2010 a 2011. RESULTADOS Houve 33.101 solicitações médicas para 268 leitos de unidade de terapia intensiva regulados no Rio de Janeiro. Com tempo médio de permanência das unidades de terapia intensiva reguladas de 11,3 dias, haveria necessidade de 595 leitos ativos para garantir a estabilidade do sistema e 628 leitos para o tempo máximo na fila de seis horas. Deduzidas as atuais taxas de desistência por melhora clínica (25,8%), estes números caem para 441 e 471. Com tempo médio de permanência de 6,5 dias, o número necessário seria de 342 e 366 leitos, respectivamente; deduzidas as taxas de desistência, de 254 e 275. O Ministério da Saúde estabelece parâmetro de 118 a 353 leitos. Embora o número de leitos regulados esteja na faixa recomendada, necessita-se incremento de 122,0% de leitos para garantir a estabilidade do sistema e de 134,0% para um tempo máximo de espera de seis horas. CONCLUSÕES O dimensionamento adequado de leitos deve considerar os motivos de limitações de acesso oportuno e a gestão do fluxo de pacientes em um cenário que associa priorização das solicitações com menor tempo médio de permanência.

ABSTRACT OBJECTIVE To estimate the required number of public beds for adults in intensive care units in the state of Rio de Janeiro to meet the existing demand and compare results with recommendations by the Brazilian Ministry of Health. METHODS The study uses a hybrid model combining time series and queuing theory to predict the demand and estimate the number of required beds. Four patient flow scenarios were considered according to bed requests, percentage of abandonments and average length of stay in intensive care unit beds. The results were plotted against Ministry of Health parameters. Data were obtained from the State Regulation Center from 2010 to 2011. RESULTS There were 33,101 medical requests for 268 regulated intensive care unit beds in Rio de Janeiro. With an average length of stay in regulated ICUs of 11.3 days, there would be a need for 595 active beds to ensure system stability and 628 beds to ensure a maximum waiting time of six hours. Deducting current abandonment rates due to clinical improvement (25.8%), these figures fall to 441 and 417. With an average length of stay of 6.5 days, the number of required beds would be 342 and 366, respectively; deducting abandonment rates, 254 and 275. The Brazilian Ministry of Health establishes a parameter of 118 to 353 beds. Although the number of regulated beds is within the recommended range, an increase in beds of 122.0% is required to guarantee system stability and of 134.0% for a maximum waiting time of six hours. CONCLUSIONS Adequate bed estimation must consider reasons for limited timely access and patient flow management in a scenario that associates prioritization of requests with the lowest average length of stay.

RESUMO OBJETIVO Determinar o número necessário de leitos públicos de unidades de terapia intensiva para adultos no estado do Rio de Janeiro para atender à demanda existente, e comparar os resultados com a recomendação do Ministério da Saúde. MÉTODOS Seguiu-se modelo híbrido que agrega séries temporais e teoria de filas para prever a demanda e estimar o número de leitos necessários. Foram considerados quatro cenários de fluxo de pacientes, de acordo com as solicitações de vagas, proporção de desistências e tempo médio de permanência no leito de unidade de terapia intensiva. Os resultados foram confrontados com os parâmetros do Ministério da Saúde. Os dados foram obtidos da Central Estadual de Regulação, de 2010 a 2011. RESULTADOS Houve 33.101 solicitações médicas para 268 leitos de unidade de terapia intensiva regulados no Rio de Janeiro. Com tempo médio de permanência das unidades de terapia intensiva reguladas de 11,3 dias, haveria necessidade de 595 leitos ativos para garantir a estabilidade do sistema e 628 leitos para o tempo máximo na fila de seis horas. Deduzidas as atuais taxas de desistência por melhora clínica (25,8%), estes números caem para 441 e 471. Com tempo médio de permanência de 6,5 dias, o número necessário seria de 342 e 366 leitos, respectivamente; deduzidas as taxas de desistência, de 254 e 275. O Ministério da Saúde estabelece parâmetro de 118 a 353 leitos. Embora o número de leitos regulados esteja na faixa recomendada, necessita-se incremento de 122,0% de leitos para garantir a estabilidade do sistema e de 134,0% para um tempo máximo de espera de seis horas. CONCLUSÕES O dimensionamento adequado de leitos deve considerar os motivos de limitações de acesso oportuno e a gestão do fluxo de pacientes em um cenário que associa priorização das solicitações com menor tempo médio de permanência.

ABSTRACT OBJECTIVE To estimate the required number of public beds for adults in intensive care units in the state of Rio de Janeiro to meet the existing demand and compare results with recommendations by the Brazilian Ministry of Health. METHODS The study uses a hybrid model combining time series and queuing theory to predict the demand and estimate the number of required beds. Four patient flow scenarios were considered according to bed requests, percentage of abandonments and average length of stay in intensive care unit beds. The results were plotted against Ministry of Health parameters. Data were obtained from the State Regulation Center from 2010 to 2011. RESULTS There were 33,101 medical requests for 268 regulated intensive care unit beds in Rio de Janeiro. With an average length of stay in regulated ICUs of 11.3 days, there would be a need for 595 active beds to ensure system stability and 628 beds to ensure a maximum waiting time of six hours. Deducting current abandonment rates due to clinical improvement (25.8%), these figures fall to 441 and 417. With an average length of stay of 6.5 days, the number of required beds would be 342 and 366, respectively; deducting abandonment rates, 254 and 275. The Brazilian Ministry of Health establishes a parameter of 118 to 353 beds. Although the number of regulated beds is within the recommended range, an increase in beds of 122.0% is required to guarantee system stability and of 134.0% for a maximum waiting time of six hours. CONCLUSIONS Adequate bed estimation must consider reasons for limited timely access and patient flow management in a scenario that associates prioritization of requests with the lowest average length of stay.

Identificar mudanças no tempo gasto em cada modo de transporte para ir à escola por sexo e idade, em adolescentes de Santa Catarina, Brasil. Dois levantamentos de base escolar foram realizados, em 2001 (N = 5.028) e 2011 (N = 6.529), com estudantes de 15-19 anos de idade. O modo de transporte (a pé, de bicicleta, de ônibus, carro/moto) e o tempo gasto para ir à escola foram analisados. O deslocamento ativo aumentou para viagens curtas em rapazes (25,1% para 36,7%) e moças (18,8% para 29,2%) e em todas as idades (15-16 anos: 21% para 32,7%; 17-19 anos: 21,9% para 32,4%); e diminuiu para viagens longas nos rapazes (30,5% para 21,9%) e adolescentes de 15-16 anos (25,7% para 34,7%). O uso de carro/moto dobrou para viagens curtas em rapazes (38,1% para 65,9%) e em estudantes de 17-19 anos (37,7% para 62,7%). Nossos resultados contribuem em discussões com foco em políticas públicas sobre ambiente seguro para promover deslocamento ativo à escola, especialmente para diminuir o uso de transporte motorizado em curtas distâncias.

To examine changes in the time spent in each mode of transportation used for going to school by gender and age among adolescents from Santa Catarina State, Brazil. Two school-based surveys were performed in 2001 (N = 5,028) and 2011 (N = 6,529) in high school students (15-19 years old). The mode of transportation (on foot; by bicycle; by bus; car/motorcycle) and the time spent for commuting to school were assessed. Active commuting increased for short trips in both genders (male: 25.1% to 36.7%; female: 18.8% to 29.2%) and in all ages (15-16 years: 21% to 32.7%; 17-19 years: 21.9% to 32.4%), and declined for longer trips in males (30.5% to 21.9%) and in 15-16 years old students (25.7% to 34.7%). Car/motorcycle use has doubled for short trips in males (38.1% to 65.9%) and in 17-19 years old students (37.7% to 62.7%), while the use of buses remained stable in both genders. Our findings contribute to discussions on public policy focusing on the design of safe environments to promote active commuting to schools, particularly to decrease the use of motorized transport for short trips.

El trabajo examina los cambios en el tiempo empleado para ir a la escuela en cada tipo de transporte, según el género y la edad de adolescentes pertenecientes al estado de Santa Catarina, Brasil. Se realizaron dos estudios epidemiológicos en 2001 (N = 5.028) y 2011 (N = 6.529) en estudiantes de instituto (15-19 años). Se midió el modo de transporte (a pie; en bicicleta; en autobús; en coche/moto) y el tiempo empleado en ir a la escuela. El transporte activo aumentó en las distancias cortas en ambos géneros (hombre: de 25,1% a 36,7%; mujer: de 18,8% a 29,2%) y en todas las edades (15-16 años: de 21% a 32.7%; 17-19 años: de 21.9% a 32.4%) y descendió en las distancias más largas en hombres (de 30,5% a 21,9%) y en estudiantes de 15-16 años (de 25,7% a 34,7%). El uso de coche/moto se duplicó en las distancias cortas en hombres (de 38,1% a 65,9%) y en estudiantes de 17-19 años (de 37,7% a 62,7%), mientras que el uso de autobús permaneció estable en ambos géneros. Nuestros resultados contribuyen a los debates de políticas públicas para disminuir el uso de transporte motorizado en viajes cortos.

Identificar mudanças no tempo gasto em cada modo de transporte para ir à escola por sexo e idade, em adolescentes de Santa Catarina, Brasil. Dois levantamentos de base escolar foram realizados, em 2001 (N = 5.028) e 2011 (N = 6.529), com estudantes de 15-19 anos de idade. O modo de transporte (a pé, de bicicleta, de ônibus, carro/moto) e o tempo gasto para ir à escola foram analisados. O deslocamento ativo aumentou para viagens curtas em rapazes (25,1% para 36,7%) e moças (18,8% para 29,2%) e em todas as idades (15-16 anos: 21% para 32,7%; 17-19 anos: 21,9% para 32,4%); e diminuiu para viagens longas nos rapazes (30,5% para 21,9%) e adolescentes de 15-16 anos (25,7% para 34,7%). O uso de carro/moto dobrou para viagens curtas em rapazes (38,1% para 65,9%) e em estudantes de 17-19 anos (37,7% para 62,7%). Nossos resultados contribuem em discussões com foco em políticas públicas sobre ambiente seguro para promover deslocamento ativo à escola, especialmente para diminuir o uso de transporte motorizado em curtas distâncias.

To examine changes in the time spent in each mode of transportation used for going to school by gender and age among adolescents from Santa Catarina State, Brazil. Two school-based surveys were performed in 2001 (N = 5,028) and 2011 (N = 6,529) in high school students (15-19 years old). The mode of transportation (on foot; by bicycle; by bus; car/motorcycle) and the time spent for commuting to school were assessed. Active commuting increased for short trips in both genders (male: 25.1% to 36.7%; female: 18.8% to 29.2%) and in all ages (15-16 years: 21% to 32.7%; 17-19 years: 21.9% to 32.4%), and declined for longer trips in males (30.5% to 21.9%) and in 15-16 years old students (25.7% to 34.7%). Car/motorcycle use has doubled for short trips in males (38.1% to 65.9%) and in 17-19 years old students (37.7% to 62.7%), while the use of buses remained stable in both genders. Our findings contribute to discussions on public policy focusing on the design of safe environments to promote active commuting to schools, particularly to decrease the use of motorized transport for short trips.

El trabajo examina los cambios en el tiempo empleado para ir a la escuela en cada tipo de transporte, según el género y la edad de adolescentes pertenecientes al estado de Santa Catarina, Brasil. Se realizaron dos estudios epidemiológicos en 2001 (N = 5.028) y 2011 (N = 6.529) en estudiantes de instituto (15-19 años). Se midió el modo de transporte (a pie; en bicicleta; en autobús; en coche/moto) y el tiempo empleado en ir a la escuela. El transporte activo aumentó en las distancias cortas en ambos géneros (hombre: de 25,1% a 36,7%; mujer: de 18,8% a 29,2%) y en todas las edades (15-16 años: de 21% a 32.7%; 17-19 años: de 21.9% a 32.4%) y descendió en las distancias más largas en hombres (de 30,5% a 21,9%) y en estudiantes de 15-16 años (de 25,7% a 34,7%). El uso de coche/moto se duplicó en las distancias cortas en hombres (de 38,1% a 65,9%) y en estudiantes de 17-19 años (de 37,7% a 62,7%), mientras que el uso de autobús permaneció estable en ambos géneros. Nuestros resultados contribuyen a los debates de políticas públicas para disminuir el uso de transporte motorizado en viajes cortos.

OBJETIVOS: Avaliar a prevalência e a circulação dos genótipos de rotavírus, antes e após a introdução da vacina oral contra rotavírus humano, bem como verificar uma possível mudança na faixa etária de ocorrência da infecção pelo RV-A. MÉTODOS: Trata-se de um estudo transversal realizado no período de 2002 a 2011, em Juiz de Fora, MG. Foram avaliados 1.144 espécimes fecais diarreicos, obtidos de crianças de 0 a cinco anos não hospitalizadas, que foram analisadas por PAGE e RT-PCR. Os dados relativos à prevalência e distribuição etária dos casos de rotavirose foram analisados pelo teste χ2 (p < 0,05), utilizando-se o programa SPSS, versão 13.0. RESULTADOS: Infecções por rotavírus foram detectadas em 9,35% (107/1.144) das amostras, com prevalências variando de 11,12% (90/809) no período pré-vacinal a 5,07% (17/335) no pós-vacinal (p = 0,001). Dentre as amostras caracterizadas, os genótipos mais frequentemente detectados foram G1P[6] (6/33 = 18,2%) no período 2002-2005 e G2P[4] no ano de 2006 (11/33 = 33,3%) e no período 2007-2011 (5/33 = 15,2%). Observou-se, ainda, uma redução significativa no número de casos de rotavirose em crianças de 0 a 36 meses, após a introdução da vacina. CONCLUSÕES: O estudo revelou queda significativa na prevalência de rotavírus, principalmente na faixa etária de 0 a 36 meses, no período 2007-2011, bem como redução na circulação do genótipo G1.

OBJECTIVES: To evaluate the prevalence and circulation of rotavirus genotypes before and after the introduction of oral vaccine against human rotavirus (OVHR), and to check for a possible change in the age of occurence of the infection by RV-A. METHODS: This was a cross-sectional study conducted between 2002-2011, in the city of Juiz de Fora, state of Minas Gerais, Brazil. A total of 1,144 diarrheal stool specimens were obtained from nonhospitalized children aged between 0 and 5 years, and analyzed by polyacrylamide gel electrophoresis and reverse-transcription polymerase chain reaction for genotype characterization. Data on prevalence and age distribution of rotavirus cases were analyzed through the chi-squared test (p < 0.05), using SPSS, release 13.0. RESULTS: Rotavirus infection was detected in 9.35% (107/1,144) samples, with prevalence rates ranging from 11.12% (90/809) in the pre-vaccine to 5.07% (17/335) in the post-vaccine period (p = 0.001). Among the samples tested, the most frequently detected genotypes were G1P[6] (6/33 = 18.2%) in the period between 2002 and 2005 and G2P[4] in 2006 (11/33 = 33.3%) and in the period between 2007 and 2011 (5/33 = 15.2%). There was also a significant reduction in the number of cases of rotavirus disease in children aged between 0 and 36 months after the vaccine introduction. CONCLUSIONS: The study evidenced a significant decrease in the prevalence of rotavirus, mainly in children aged between 0 and 36 months in the 2007-2011 period, as well as a reduction in G1 genotype circulation.

This retrospective study (April-September 2003) was designed to investigate the roles of the main viruses responsible for cases of acute infantile gastroenteritis in hospitalised children up to two years of age. The viruses were identified in 64.7% (88/136) of the cases and the detection rates of rotavirus A (RVA), norovirus (NoV) and astrovirus were 41.9% (57/136), 30.3% (24/79) and 12.7% (7/55), respectively. RVA and NoV were detected in 20 of the 24 reported nosocomial infection cases. This study identified the first circulation of the genotype NoV GII.21 in Brazil and highlights the need to establish differential diagnoses through active laboratorial surveillance.

Rotavirus is a major cause of infantile acute diarrhea, causing about 440,000 deaths per year, mainly in developing countries. The World Health Organization has been recommending the assessment of rotavirus burden and strain characterization as part of the strategies of immunization programs against this pathogen. In this context, a prospective study was made on a sample of 134 children with acute diarrhea and severe dehydration admitted to venous fluid therapy in two state hospitals in Rio de Janeiro, Brazil, from February to September 2004. Rotavirus where detected by polyacrylamide gel electrophoresis (PAGE) and by an enzyme-linked immunoassay to rotavirus and adenovirus (EIARA) in 48% of the children. Positive samples for group A rotavirus (n = 65) were analyzed by reverse transcription/heminested multiplex polymerase chain reaction to determine the frequency of G and [P] genotypes and, from these, 64 samples could be typed. The most frequent G genotype was G1 (58%) followed by G9 (40%). One mixed infection (G1/G9) was detected. The only [P] genotype identified was [8]. In order to estimate the rotavirus infection frequency in children who acquired diarrhea as hospital infection in those hospitals, we studied 24 patients, detecting the pathogen in 41% of them. This data suggest that genotype G9 is an important genotype in Rio de Janeiro, with implications to the future strategies of vaccination against rotavirus, reinforcing the need of continuous monitoring of circulating strains of the pathogen, in a surveillance context.