ABSTRACT Regenerated cellulose properties can be altered and manipulated by various methods and procedures. Coagulation steps in cellulose regeneration process is one of the most crucial step in determining the final properties of the regenerated cellulose products. This study was conducted to investigate the effect of different type of coagulant on the properties of regenerated cellulose membranes (RCM). Hence, the coagulation mechanism will be highlighted and postulated. Cellulose from cotton linter was dissolved in NaOH/urea via precooled method to form cellulose solution and coagulated in three types of coagulant which were distilled water, 5 wt% H2SO4 and 5 wt% (NH4)2SO4 which lead to the formation of RCM. The RCM were air dried and characterized for their physical, mechanical and chemical properties by using X-ray diffraction (XRD), tensile and Fourier transform infrared (FTIR). Results showed that RCM coagulated in distilled water has the highest tensile strength and crystallinity index but showed the lowest value for the elongation at break. Meanwhile, the RCM coagulated in 5 wt% (NH4)2SO4 has the lowest tensile strength and crystallinity index but had the highest value of the elongation at break. From these results, the crystallinity of RCM has affected the elongation at break for all sample. RCM with higher crystallinity have lower elongation at break compared to the one with higher crystallinity. The RCM coagulated in 5 wt% H2SO4 has intermediate results for all tensile strength, elongation at break and crystallinity compared to those coagulated in distilled water and 5 wt% (NH4)2SO4.