ABSTRACT Objective: Transient receptor potential melastatin (TRPM) are integral membrane proteins that have broad range of cellular functions. Roles of TRPM2, TRPM3, TRPM4 and TRPM7 among these channels are very important, and their roles in lung ischaemia/reperfusion injury have not been evaluated yet. The aim of this study is to investigate the contribution of these genes in lung ischaemia/reperfusion injury and evaluate histopathology of tissues. Methods: A total of 40 Wistar albino rats were enrolled for the study. Ischaemia was performed by the application of an atramvatic clamp to pulmonary artery. Gene expressions were determined by the semi-quantitative reverse transcription-polymerase chain reaction method. Histopatholical evaluations were held by a standard haematoxyline–eosin staining. Results: The major histopathological tissue damage was observed in ischaemia performed groups, and expression of TRPM channels was found to be obviously downregulated. Substantial changes were determined between TRPM2, TRPM3, TRPM4 and TRPM7 and lung ischaemia/reperfusion injury. In particular, expression of TRPM2 and TRPM7 was reversibly downregulated in ischaemia. Yet, the expression of TRPM3 and TRPM4 was irreversibly down-regulated after ischaemia. Conclusion: Consequently, these results indicate that TRPM family of cation channels may have significant roles in the lung ischaemia/reperfusion injury.

ABSTRACT This study investigated the effects of the addition of Lactobacillus reuteri E81 (LRE) into rations of chukar partridges (Alectoris chukar) fed under heat stress (HS) conditions on fattening performance and meat quality. This study included 256 chukar partridges aged 1 day. The study comprised an adaptation period of 7 days and a fattening period of 35 days and included 8 different groups with 32 animals in each group. Each group was further divided into four subgroups with eight animals in each subgroup. At the end of the study, the best results in terms of fattening performance in the non-HS groups were obtained in the LRE 600 ppm group and in the HS groups, SLRE 200 ppm had the best effect on average live weight and average live weight increase, whereas SLRE 400 ppm had the best effect on FCR (p<0.05). The analysis of the samples collected from chukar partridges on day 21 showed that, there was no effect on the colour parameters and Thiobarbituric acid reactive substances (TBARS) level in the LRE in the HS and non- HS groups, whereas the meat pH level decreased in the SLRE 400 ppm group (p<0.05). The analysis of the samples collected on day 42 showed that there was no effect on colour parameters in the HS and non-HS groups. TBARS level decreased at the dose of LRE 200 ppm in the non-HS group, and the meat pH level decreased in both HS and non-HS groups (p<0.05).

ABSTRACT Cardiac troponin (cTnI) and erythrocyte distribution width (RDW) have been used as specific markers for the evaluation of cardiac arrhythmias and myocardial diseases. In this experimental study, we aimed to determine the dose-dependent administration of an Essential OilMixture (EOM) (Eucalyptus globulusLabill, Thymus vulgaris, Cymbopogon nardus, and Syzygiumaromaticum) on the serum erythrocyte indices and myocardial damage. We used 400 male, one-day-old, Ross-308 chicks. Each group ((n=8) (22°C Control (C), C+250 mL/1,000 L, C+500 mL/1,000 L, C+750 mL/1,000 L), 36°C (stress control (SC), SC+250 mL/1,000 L, SC+500 mL/1,000 L, SC+750 mL/1,000 L) was further divided into eight groups with 50 animals. Each group of 50 chicks was further subdivided into five groups with 10 animals in each compartment. The results showed that the WBC, RBC, HGB, HCT, RDW-SD, RDW-CV, and Lymphocyte decreased in groups without exposure to temperature stress (22ºC) compared to the 22ºC control group; whereas, CnTnI, MCHC, NEUT, and IG (p<0.05) increased compared to the control group. In groups exposed to temperature stress (36ºC), the WBC, HGB, HCT, RDW-SD, and Lymphocyte decreased compared to the control group, but MCH, MCHC, NEUT, and IG increased compared to the control. However, cTnI, CK, creatinine, RBC, and RDW had no effect on CD, MONO, EO, and BASO (p<0.05). EOM mixture had no effect on hematological and biochemical parameters.