ABSTRACT Laetia suaveolens (Poepp.) Benth., Salicaceae, popularly known as "casinga-cheirosa", "caferana", or "laranjinha", is native to Brazil but not endemic to this country. A crude organic extract was obtained from the leaves and stem and intraperitoneally administered in male Balb-c mice. Its behavioral effects were evaluated in the open field and elevated plus maze in a two-stage experiment that assessed ten different parameters related to behavior as locomotion, emotionality, and anxiety. In the first stage of the experiment, intraperitoneal the crude organic extract administration dose-dependently impaired locomotion and emotionality 30–120 min after administration. A significant decrease in defecation was observed, which was related to emotionality. No alterations in the elevated plus maze were found; thus, this apparatus was not used in the next stage of the experiment. In the second stage, the previously determined non-lethal dose of 0.1563 g/kg was intraperitoneally administered, which impaired locomotion and rearing frequency and increased immobility time. Necropsy revealed smooth intestine hemorrhage. Rutin, leucoside, nicotiflorin, guaijaverin, and astragalin were isolated from the crude organic extract. This is the first time that these compounds have been identified in L. suaveolens. In conclusion, the crude organic extract impaired locomotion and emotionality and caused hemorrhage in male Balb-c mice, indicating that its consumption can be harmful to humans and animals. The present results provide a basis for further studies on the pharmacology, toxicology, and natural product chemistry of the crude organic extract.

The organic extract EB689, obtained from the stem of Abarema auriculata (Benth.) Barneby & J.W.Grimes, Fabaceae, commonly known as "saboeiro-ferro", was chemically studied, as well as its influence over behavioral effects such as locomotion, emotionality and anxiety, after intra-peritonial administration were assessed. The open-field and elevated-plus maze were used in experiments divided into two stages. The first stage aimed for the identification of the main effects over behavior using a reduced number of animals against half-fold diluted doses of EB689. The same variables were also tested in a second stage of the experiment using the non-lethal intra-peritoneal dose of 4.8 mg/kg in a larger number of animals. It was observed that EB689 clearly decreased locomotion, which was probably caused by internal hemorrhage causing hypovolemic shock. Although it is the first time lupeol and eucryphin are described in A. auriculata, it is still not clear if they are involved in the toxicology of A. auriculata. The undesirable effects of EB689 are better understood, the basis for further pharmacological assays aiming antitumor activity are supported.

OBJETIVO: Comparar o estado imunológico de 44 pacientes pediátricos com fibrose cística (FC)a umgrupo-controle formado por 16 indivíduos saudáveis. MÉTODOS: Foram selecionados para o estudo pacientes com FC com idade entre 3 e 12 anos, apresentando um escore clínico moderado e bom. Foram avaliados a glutationa eritrocitária, a produção de espécies reativas de oxigênio, citocinas (TNF-α, IFN-γ, IL-8, IL-6, IL-10) em culturas de células mononucleares do sangue periférico em condições espontâneas e estimuladas por BCG ou PHA, a concentração sérica de TGF-β2, IgA, IgG, IgM, IgE e IgA salivar. RESULTADOS :A produção espontânea de TNF-α, IL-6 e IL-10, a produção de IL-6 estimulada por PHA e TGF-β2, IgA e IgG séricas aumentaram em amostras de pacientes com FC. Indivíduos saudáveis tiveram uma produção mais elevada de TNF-α em resposta a BCG. CONCLUSÃO: Apesar de os pacientes com FC parecerem clinicamente estáveis, os resultados de seus exames de sangue periférico mostraram que houve um impacto sobre o sistema imunológico.

OBJECTIVE: To compare the immunologic state of 44 pediatric patients with cystic fibrosis (CF) with a control group consisting of 16 healthy individuals. METHODS: CF patients aged 3 to 12 years with moderate to good clinical score were selected for the study. Erythrocytic glutathione, production of reactive oxygen species, cytokines (TNF-α, IFN-γ, IL-8, IL-6, IL-10) in peripheral blood mononuclear cells cultures under spontaneous and BCG- or PHA-stimulated conditions, serum concentrations of TGF-β2, IgA, IgG, IgM, IgE, and salivary IgA were evaluated. RESULTS: The spontaneous production of TNF-α, IL-6, and IL-10, the PHA-stimulated production of IL-6, and the serum TGF-β2, IgA, and IgG were increased in samples from CF patients. Healthy subjects had a higher production of TNF-α in response to BCG. CONCLUSION: Although CF patients appearedclinically stable, the results of their peripheral blood examinations demonstrated an impact on the immune system.

A quantitative and descriptive study, aimed at verifying the knowledge of women who have sex with women on ways of transmission, prevention and risk factors regarding to human papillomavirus (HPV). It was conducted with 31 women living in the city of Sao Jose do Rio Preto, São Paulo. Data were collected through structured questionnaire with 17 questions analyzed with the aid of the Statistical Package of Social Sciences and descriptive statistics. It was found that the average age of women was 39.5 years, and that 52% had between 9 and 11 years of study. Regarding knowledge, 68% of women did not know the significance of HPV, 58% believed that condom protects fully 45% responded that Pap smears should be performed twice a year. It was concluded that there is a vulnerability to contamination due to exposure to HPV risk factors and lack of knowledge.

Estudio cuantitativo y descriptivo, que tuvo como objetivo verificar el conocimiento de mujeres que hacen sexo con mujeres acerca de las formas de transmisión, prevención y factores de riesgo del papiloma virus hunano (HPV). Fue realizado con 31 mujeres residentes en municipio de São José do Rio Preto, estado de São Paulo. Los datos fueron recogidos por medio de cuestionario estructurado con 17 cuestiones, analizados con auxilio del programa estadístico Statístical Package of Social Sciences y estadística descriptiva. Se ha constatado que la media de edad de las mujeres fue de 39,5 años, y 52% poseían entre 9 y 11 años de estudio. En relación al conocimiento, 68% de las mujeres no sabían el significado del HPV, 58% creían que el condón protege totalmente, 45% contestaron que el Papanicolau debe ser realizado dos veces al año. La conclusión es que hay vulnerabilidad para contaminación por HPV a causa de la exposición a los factores de riesgo y falta de conocimiento.

Estudo quantitativo e descritivo, que teve como objetivo verificar o conhecimento de mulheres que fazem sexo com mulheres sobre as formas de transmissão, prevenção e fatores de risco relacionados ao papiloma vírus humano (HPV). Foi realizado com 31 mulheres residentes no Município de São José do Rio Preto, Estado de São Paulo. Os dados foram coletados por meio de questionário estruturado com 17 questões, analisados com auxílio do programa estatístico Statistical Package of Social Sciences e estatística descritiva. Constatou-se que a média de idade das mulheres foi de 39,5 anos, e que 52% possuíam entre 9 e 11 anos de estudo. Em relação ao conhecimento, 68% das mulheres não sabiam o significado do HPV, 58% acreditavam que a camisinha protege totalmente, e 45% responderam que o Papanicolau deve ser realizado duas vezes ao ano. Concluiu-se que há vulnerabilidade para contaminação por HPV devido à exposição aos fatores de risco e falta de conhecimento.

Neste trabalho foi investigado o efeito inibitório de extratos etanólicos de partes aéreas de Hypericum myrianthum e Hypericum polyanthemum sobre a germinação e o crescimento de alface (Lactuca sativa). A germinação das sementes foi reduzida em todas as concentrações avaliadas. Após sete dias, o percentual de germinação foi significativamente retardado pelos extratos de ambas as plantas, nas maiores concentrações, em comparação com o grupo controle. O comprimento das radículas foi significativamente afetado, estas apresentando alguns sinais de necrose. As duas espécies apresentam compostos fenólicos como constituintes principais, os quais podem ser responsáveis pela inibição da germinação e crescimento de Lactuca sativa.

Germination and growth inhibitory effects of ethanolic crude extracts of Hypericum myrianthum and H. polyanthemum aerial parts on lettuce (Lactuca sativa) were investigated. The germination was retarded in all the tested concentrations. After seven days the final germination percentage of the most concentrated extracts was significantly reduced by both extracts in comparison with the control. The radicles length was significantly affected showing necrosis. Both species present phenolic compounds as the main components and they could be responsible for the inhibition of the germination and growth of Lactuca sativa.