Abstract Objective: The aim of this study was to verify the adequacy of affordances in the home environment of children at risk of developmental delay and to identify factors associated with their frequency. Methods: The cross-sectional study included 97 families who responded to the Affordances in the Home Environment for Motor Development — Infant Scale (AHEMD-IS) for 3–18 months (n=63), or AHEMD – Self-Report (AHEMD-SR) for 18–42 months (n=34). The Mann-Whitney U test was used to identify the differences between the frequencies of affordances between the groups. Multiple linear regression was used to verify the association between the child’s sex, mother’s marital status, education, socioeconomic level, child and mother’s ages, house residents’ number, per capita income, and AHEMD scores (α=0.05). Results: The home affordances’ frequency in the AHEMD-IS ranged from less than adequate to excellent, while in the AHEMD-SR, the highest predominance was medium. The offer of stimuli in the AHEMD-IS was significantly higher. Higher socioeconomic level and house residents’ number were associated with greater affordances. Conclusions: The higher the socioeconomic level and house residents’ number, the greater the affordances in the homes of children at risk of delay. It is necessary to provide families with some alternatives to make their home environments richer in affordances that favor child development.

RESUMO Objetivo Verificar a adequação das oportunidades de ação no ambiente doméstico de crianças em risco de atraso no desenvolvimento e identificar fatores associados à sua frequência. Métodos Este estudo transversal incluiu 97 famílias que responderam ao Affordances in the Home Environment for Motor Development - Infant Scale (AHEMD-IS) para 3–18 meses (n=63) ou o Self Report (AHEMD-SR) para 18–42 meses (n=34). O teste de Mann-Whitney foi utilizado para identificar as diferenças entre as frequências de oportunidades de ação entre os grupos. A regressão linear múltipla foi utilizada para verificar a associação entre o sexo da criança, o estado civil, a escolaridade e nível socioeconômico da mãe, as idades da criança e da mãe, o número de residentes da casa, a renda per capita e os escores do AHEMD (α=0,05). Resultados A frequência das oportunidades de ação dos domicílios no AHEMD-IS variou de menos do que adequado a excelente; enquanto, no AHEMD-SR, a maior predominância foi de média frequência. A oferta de estímulos no AHEMD-IS foi significativamente maior. O maior nível socioeconômico e o número de moradores da casa foram associados a maiores oportunidades de ação. Conclusões Quanto maior o nível socioeconômico e o número de moradores, maiores são as oportunidades de ação nos domicílios das crianças em risco de atraso. É necessário oferecer às famílias algumas alternativas para tornar os seus ambientes domésticos mais ricos em oportunidades de ação que favoreçam o desenvolvimento infantil.

Abstract Microcosm biofilms can reproduce the complexity of a dental biofilm. However, different forms of cultivation have been used. The impact of the culture atmosphere on the development of microcosm biofilms and their potential to cause tooth demineralization has not yet been deeply studied. Objective This study analyzes the effects of three experimental cultivation models (microaerophile vs. anaerobiosis vs. experimental mixed) on the colony-forming units (CFU) of the cariogenic microorganisms and tooth demineralization. Methodology 90 bovine enamel and 90 dentin specimens were distributed into different atmospheres: 1) microaerophilia (5 days, 5% CO2); 2) anaerobiosis (5 days, jar); 3) mixed (2 days microaerophilia and 3 days anaerobiosis), which were treated with 0.12% chlorhexidine (positive control – CHX) or Phosphate-Buffered Saline (negative control – PBS) (n=15). Human saliva and McBain’s saliva containing 0.2% sucrose were used for microcosm biofilm formation, for 5 days. From the second day to the end of the experiment, the specimens were treated with CHX or PBS (1x1 min/day). Colony-forming units (CFU) were counted, and tooth demineralization was analyzed using transverse microradiography (TMR). Data were subjected to two-way ANOVA and Tukey’s or Sidak’s test (p<0.05). Results CHX was able to reduce total microorganism’s CFU compared to PBS (differences of 0.3–1.48 log10 CFU/mL), except for anaerobiosis and microaerophilia in enamel and dentin biofilm, respectively. In the case of dentin, no effect of CHX on Lactobacillus spp. was observed. CHX significantly reduced enamel demineralization compared to PBS (78% and 22% reductions for enamel and dentin, respectively). Enamel mineral loss did not differ when compared with the other atmospheres; however, the enamel lesion depth was greater under anaerobiosis. Dentin mineral loss was lower under anaerobiosis when compared with the other atmospheres. Conclusion The type of atmosphere has, in general, little influence on the cariogenic ability of the microcosm biofilm.

Abstract The initial characteristics of white spot lesion (WSLs), such as the degree of integrated mineral loss (ΔZ), depth and pattern of mineral distribution, have an impact on further demineralization and remineralization. However, these lesion parameters have not been evaluated in WSLs produced from microcosm biofilms. Objective: This study characterized artificial white spot lesions produced on human enamel under microcosm biofilm for different experimental periods. Methodology: In total, 100 human enamel specimens (4x4mm) were assigned to 5 distinct groups (n=20/group) differing according to the period of biofilm formation (2, 4, 6, 8 or 10 days). Microcosm biofilm was produced on the specimens from a mixture of human and McBain saliva at the first 8h. Enamel samples were then exposed to McBain saliva containing 0.2% sucrose. WSLs formed were characterized by quantitative light-induced fluorescence (QLF) and transverse microradiography (TMR). Data were analyzed by ANOVA/Tukey or Kruskal-Wallis/Dunn tests (p<0.05). Results: A clear time-response pattern was observed for both analyses, but TMR was able to better discriminate among the lesions. Regarding QLF analysis, median (95%CI; %) changes in fluorescence ∆Z were -7.74(-7.74:-6.45)a, -8.52(-8.75:-8.00)ab, -9.17(-10.00:-8.71)bc, -9.58(-10.53:-8.99)bc and -10.01(-11.44:-9.72)c for 2, 4, 6, 8, and 10 days, respectively. For TMR, median (95%CI; vol%.µm) ∆Z were 1410(1299-1479)a, 2420(2327-2604)ab, 2775(2573-2899)bc, 3305(3192-3406)cd and 4330(3972-4465)d, whereas mean (SD; µm) lesion depth were 53.7(12.3)a, 71.4(12.0)a, 103.8(24.8)b, 130.5(27.2)bc, 167.2(39.3)c for 2, 4, 6, 8 and 10 days, respectively. Conclusion: The progression of WSLs formed on human enamel under microcosm biofilm can be characterized over 2-10 days, both by QLF and TMR analyses, although the latter provides better discrimination among the lesions.

Abstract Chronic Lymphocytic Leukemia (CLL) has shown great biological heterogeneity, with a variable prognosis, and a short survival in some patients. In this light, the present study focused on the prognostic utility of circulating microparticles (MPs) and a Thrombin Generation (TG) Profile for thrombotic risk and disease progression in CLL patients. Circulating microparticles and TG were evaluated in 35 patients with CLL and 35 healthy individuals. For circulating microparticles, significant differences were observed among the following groups: MPs derived from endothelial cells (p = 0.002), B lymphocytes (p < 0.001), platelets (p = 0.003), and Tissue Factor MPs in monocytes (p < 0.001). In all cases, MP values were higher for the CLL group. When compared to the controls, CLL patients presented a decrease in TG, characterized by a reduced endogen thrombin potential (ETP) (p = 0.031). When the results were analyzed according to the Binet stage, as compared to the controls, the Binet B+C group also presented lower ETP values (p = 0.009). No significant differences were observed between the control and the Binet A groups or between the Binet A and the Binet B + C groups. Although hemostatic alterations may occur in patients with CLL, these parameters do not seem to be useful to indicate disease progression.

Abstract Quantification of collagen degradation is an important parameter to evaluate dentin caries for preventive aid. Objectives: Evaluate preventive methods against root collagen degradation by the hydroxyproline assay (HYP) and microradiography technique (MRT). Methodology: Five bovine root dentin blocks were obtained and subjected to an artificial demineralization process by acetate buffer (pH 5) to induce carious lesion formation. Samples were subjected to the following therapeutic treatments: 1) 0.12% chlorhexidine for 1 min, 2) 2% fluoride for 1 min, 3) Nd:YAG Laser (400 μm diameter optical fiber, 10 Hz frequency, 60 mJ/pulse energy, 48 J/cm2 energy density, in noncontact mode for 10 s), 4) deionized water (control) for 1 min, 5) MRT control group (without treatment and removal of collagen). Samples were exposed to degradation by a collagenase enzyme for five days. The enzyme solution was collected, by colorimetry in a spectrophotometer, from the collagen matrix for the hydroxyproline release analysis. The same samples were subjected to an additional two days of demineralization to induce the progression of mineral loss. Samples were analyzed by MRT for the visualization of their degraded areas (estimation of lesion depth and mineral loss). ANOVA was applied to compare hydroxyproline release rates. MRT data were subjected to the Kruskal-Wallis test, followed by the Dunn’s test. Comparisons between the initial five-day and the subsequent two-day demineralization processes were performed by repeated t-test or Wilcoxon (p<0.05) measurements. Results: The amount of HYP released from the dentin samples failed to show significant differences among the groups (p=0.09). Fluoride and chlorhexidine were able to interact with the samples, reducing the progression of dentin caries after removal of the demineralized organic matrix. CHX was the only treatment able to show significant lower lesion depth than the negative control. Conclusion: Chlorhexidine and fluoride were effective in reducing root caries progression.

Abstract Chronic lymphocytic leukemia (CLL) is a blood cancer characterized by the accumulation of clonal B-lymphocytes. This study evaluated the mRNA gene expression of miR-15a, miR-16- 1, ZAP-70, and Ang-2 by qPCR, as well as the plasma levels of Bcl-2 by Elisa immunoassay, in CLL patients and healthy controls. Significant differences were observed when comparing patients and controls regarding miR-15a (p < 0.001), miR-16-1 (p < 0.001) mRNA, Ang-2 gene expression, and Bcl-2 plasma levels (p < 0.001). When stratified by risk, differences were maintained with a significantly reduced expression in high-risk patients. A positive correlation was observed between miR-15a and platelets (R2 = 0.340; p = 0.009) as well as between Bcl-2 and leukocytes (R2 = 0.310; p = 0.019). Conversely, negative correlations were observed between ZAP-70 and platelets (R2 = - 0.334; p = 0.011), between miR-15a and lymphocytes (R2 = - 0.376; p = 0.004), as well as between miR-16-and lymphocytes (R2 = - 0.515; p = 0.00004). The data suggest that a reduction in miR-15a and miR-16-1 expressions, in addition to an overexpression of Bcl-2, are associated with the reduction in apoptosis and, consequently, to a longer survival of lymphocytes, thus contributing to lymphocyte accumulation and aggravation of the disease. By contrast, Ang-2 expression was significantly higher in A than in B + C Binet groups. This context leads to the speculation that this biomarker should be investigated in more robust studies within populations with a still relevantly indolent form of the disease in an attempt to identify those patients with a greater potential for an aggravation of the disease.

Abstract: This randomized three-armed controlled clinical trial compared the effect of titanium tetrafluoride (TiF4) and sodium fluoride (NaF) varnishes on caries control in smooth surfaces of permanent dentition and children's acceptability. Sixty children (6-8 y/o) were randomly divided into TiF4 (2.45% F–), NaF (2.26% F–) or placebo (control) groups. Varnishes were applied on permanent teeth once a week for the first 4 weeks and after the 6th and 12th months of the study. The variables were as follows: International Caries Detection and Assessment System (ICDAS) scores, quantitative fluorescence changes, visual plaque index (VPI) and degree of acceptability. Two-way RM-ANOVA, ANOVA/Tukey and χ2 tests were performed (p < 0.05). No differences were found between the treatments with respect to ICDAS scores (p = 0.32). Only TiF4 reduced the mean fluorescence loss significantly at 18 months compared to the baseline (p = 0.003). TiF4 showed a lower percentage of new caries lesions by tooth surface than the placebo, while NaF did not induce such a change (p < 0.014). Regardless of the treatment, more than 95% of the participants reported being satisfied. For all groups, the VPI decreased significantly at 3 months compared to the baseline value (p < 0.001), with no differences between the treatments (p = 0.17). TiF4 had a similar ability to control caries lesions as NaF; however, only TiF4 differed from the placebo (p = 0.004). The acceptability of TiF4 varnish was similar to that of NaF varnish.

RESUMO Objetivo: reconhecer as aldeias como territórios de cuidado e resistência cotidiana à marginalização social. Métodos: estudo descritivo, de abordagem qualitativa, apoiado no referencial metodológico da etnografia inspirada na Antropologia Interpretativa. Pesquisa de campo de dois anos (do segundo semestre de 2015 ao primeiro semestre de 2017). Utilizou-se observação participante, entrevista semiestruturada com quatro interlocutores-privilegiados e diário de campo. A sistematização dos dados foi realizada durante todo o trabalho de campo. Resultados: divide-se em três categorias: Os interlocutores falam sobre a vida nas aldeias; As aldeias como dispositivo de cuidado; e As drogas nas aldeias. Considerações finais: os resultados revelam o desafio que é para a saúde e a enfermagem reconhecerem a necessidade de entender os contextos de vida urbana das pessoas em situação de rua. O enfermeiro necessita abranger elementos culturais em seus processos de trabalho, promovendo vínculo e compreensão dos modos de vida das pessoas em situação de rua.

ABSTRACT Objective: to recognize villages as territories of care and daily resistance to social marginalization. Methods: a descriptive study with a qualitative approach based on the methodological framework of ethnography inspired by Interpretative Anthropology. Two-year field research (from the second half of 2015 to the first half of 2017). Participant observation and semi-structured interviews with four privileged interlocutors and a field diary have been used. Data systematization was carried out throughout field work. Results: three categories emerged: Interlocutors talking about their life in villages; Villages as a care device; and Drugs in villages. Final considerations: the results reveal the challenge for health and nursing to recognize the need to understand the contexts of urban life of homeless persons. Nurses need to include cultural elements in their work processes, promoting bonding and understanding the ways of life of homeless persons.

RESUMEN Objetivo: reconocer a los pueblos como territorios de atención diaria y resistencia a la marginación social. Métodos: estudio descriptivo, con enfoque cualitativo, apoyado por el marco metodológico de la etnografía inspirado en la antropología interpretativa. Investigación de campo de dos años (desde la segunda mitad de 2015 hasta la primera mitad de 2017). Se utilizó observación participante, entrevistas semiestructuradas con cuatro interlocutores privilegiados y un diario de campo. La sistematización de los datos se realizó a lo largo del trabajo de campo. Resultados: se divide en tres categorías: Los interlocutores hablan de la vida en los pueblos; Los pueblos como dispositivo de cuidado; y Las drogas en los pueblos. Consideraciones finales: los resultados revelan el desafío para la salud y la enfermería de reconocer la necesidad de comprender los contextos de la vida urbana de las personas sin hogar. Las enfermeras deben incluir elementos culturales en sus procesos de trabajo, promoviendo lazos y la comprensión de las formas de vida de las personas sin hogar.

ABSTRACT Objective: to recognize villages as territories of care and daily resistance to social marginalization. Methods: a descriptive study with a qualitative approach based on the methodological framework of ethnography inspired by Interpretative Anthropology. Two-year field research (from the second half of 2015 to the first half of 2017). Participant observation and semi-structured interviews with four privileged interlocutors and a field diary have been used. Data systematization was carried out throughout field work. Results: three categories emerged: Interlocutors talking about their life in villages; Villages as a care device; and Drugs in villages. Final considerations: the results reveal the challenge for health and nursing to recognize the need to understand the contexts of urban life of homeless persons. Nurses need to include cultural elements in their work processes, promoting bonding and understanding the ways of life of homeless persons.

RESUMEN Objetivo: reconocer a los pueblos como territorios de atención diaria y resistencia a la marginación social. Métodos: estudio descriptivo, con enfoque cualitativo, apoyado por el marco metodológico de la etnografía inspirado en la antropología interpretativa. Investigación de campo de dos años (desde la segunda mitad de 2015 hasta la primera mitad de 2017). Se utilizó observación participante, entrevistas semiestructuradas con cuatro interlocutores privilegiados y un diario de campo. La sistematización de los datos se realizó a lo largo del trabajo de campo. Resultados: se divide en tres categorías: Los interlocutores hablan de la vida en los pueblos; Los pueblos como dispositivo de cuidado; y Las drogas en los pueblos. Consideraciones finales: los resultados revelan el desafío para la salud y la enfermería de reconocer la necesidad de comprender los contextos de la vida urbana de las personas sin hogar. Las enfermeras deben incluir elementos culturales en sus procesos de trabajo, promoviendo lazos y la comprensión de las formas de vida de las personas sin hogar.

RESUMO Objetivo: reconhecer as aldeias como territórios de cuidado e resistência cotidiana à marginalização social. Métodos: estudo descritivo, de abordagem qualitativa, apoiado no referencial metodológico da etnografia inspirada na Antropologia Interpretativa. Pesquisa de campo de dois anos (do segundo semestre de 2015 ao primeiro semestre de 2017). Utilizou-se observação participante, entrevista semiestruturada com quatro interlocutores-privilegiados e diário de campo. A sistematização dos dados foi realizada durante todo o trabalho de campo. Resultados: divide-se em três categorias: Os interlocutores falam sobre a vida nas aldeias; As aldeias como dispositivo de cuidado; e As drogas nas aldeias. Considerações finais: os resultados revelam o desafio que é para a saúde e a enfermagem reconhecerem a necessidade de entender os contextos de vida urbana das pessoas em situação de rua. O enfermeiro necessita abranger elementos culturais em seus processos de trabalho, promovendo vínculo e compreensão dos modos de vida das pessoas em situação de rua.

RESUMO Objetivo: Analisar os meios de trabalho do enfermeiro utilizados na articulação da Rede de Atenção Psicossocial (RAPS). Método: Estudo qualitativo exploratório-descritivo, realizado na RAPS de uma Gerência Distrital de saúde de Porto Alegre. Utilizou-se como técnica de coleta de dados a entrevista semiestruturada, realizada no período de outubro a dezembro de 2017. Os dados foram submetidos à análise temática sob referencial marxista do Processo de Trabalho. Resultados: A análise resultou em duas categorias temáticas: Gestão do Cuidado e Práticas de Cuidado. A primeira é composta por dois meios de trabalho: coordenação de equipe e reuniões com os demais componentes do território. A segunda, envolve três meios de trabalho: contatos telefônicos com serviços da RAPS; encaminhamentos do usuário na RAPS e apoio matricial. Considerações finais: Tendo em vista a superação do modelo manicomial, os meios de trabalho dos enfermeiros são instrumentos de produção de diálogos que visam ampliar o cuidado psicossocial.

ABSTRACT Objective: To analyze nurses' means of work used in the articulation of the Psychosocial Care Network (PCN) Method: An exploratory-descriptive qualitative study conducted at the PCN of a District Health Management in Porto Alegre. The data collection technique used was the semi-structured interview held from October to December 2017. Data was subjected to thematic analysis under the Marxist theory of the Labor Process. Results: The analysis resulted in two thematic categories: Care Management and Care Practices. The first consists of two means of work: team coordination and meetings with the other components of the territory. The second involves three means of work: telephone contacts with PCN services; user referrals in the PCN and matrix support. Final considerations: In view of overcoming the asylum model, nurses' means of work are instruments for producing dialogs aimed at expanding psychosocial care.

RESUMEN Objetivo: Analizar los medios de trabajo utilizados por los enfermeros en la articulación de la Red de Atención Psicosocial (RAPS). Método: Estudio cualitativo, exploratorio y descriptivo realizado en la RAPS de un Distrito de Gestión de la Salud en Porto Alegre. La técnica de recolección de datos utilizada fue la entrevista semiestructurada, de octubre a diciembre de 2017. Los datos se sometieron al análisis temático bajo la teoría marxista del proceso de trabajo. Resultados: El análisis derivó en dos categorías temáticas: gestión de la atención y prácticas de atención. El primero consiste en dos medios de trabajo: coordinación del equipo de trabajo y reuniones con los otros componentes del territorio. El segundo implica tres medios de trabajo: contactos telefónicos con servicios de la R APS; referencias de usuarios en la RAPS y soporte de matriz. Consideraciones finales: En vista de superar el modelo de los manicomios, los medios de trabajo de los enfermeros son instrumentos para producir diálogos destinados a expandir la atención psicosocial.

ABSTRACT Objective: To analyze nurses' means of work used in the articulation of the Psychosocial Care Network (PCN) Method: An exploratory-descriptive qualitative study conducted at the PCN of a District Health Management in Porto Alegre. The data collection technique used was the semi-structured interview held from October to December 2017. Data was subjected to thematic analysis under the Marxist theory of the Labor Process. Results: The analysis resulted in two thematic categories: Care Management and Care Practices. The first consists of two means of work: team coordination and meetings with the other components of the territory. The second involves three means of work: telephone contacts with PCN services; user referrals in the PCN and matrix support. Final considerations: In view of overcoming the asylum model, nurses' means of work are instruments for producing dialogs aimed at expanding psychosocial care.

RESUMEN Objetivo: Analizar los medios de trabajo utilizados por los enfermeros en la articulación de la Red de Atención Psicosocial (RAPS). Método: Estudio cualitativo, exploratorio y descriptivo realizado en la RAPS de un Distrito de Gestión de la Salud en Porto Alegre. La técnica de recolección de datos utilizada fue la entrevista semiestructurada, de octubre a diciembre de 2017. Los datos se sometieron al análisis temático bajo la teoría marxista del proceso de trabajo. Resultados: El análisis derivó en dos categorías temáticas: gestión de la atención y prácticas de atención. El primero consiste en dos medios de trabajo: coordinación del equipo de trabajo y reuniones con los otros componentes del territorio. El segundo implica tres medios de trabajo: contactos telefónicos con servicios de la R APS; referencias de usuarios en la RAPS y soporte de matriz. Consideraciones finales: En vista de superar el modelo de los manicomios, los medios de trabajo de los enfermeros son instrumentos para producir diálogos destinados a expandir la atención psicosocial.

RESUMO Objetivo: Analisar os meios de trabalho do enfermeiro utilizados na articulação da Rede de Atenção Psicossocial (RAPS). Método: Estudo qualitativo exploratório-descritivo, realizado na RAPS de uma Gerência Distrital de saúde de Porto Alegre. Utilizou-se como técnica de coleta de dados a entrevista semiestruturada, realizada no período de outubro a dezembro de 2017. Os dados foram submetidos à análise temática sob referencial marxista do Processo de Trabalho. Resultados: A análise resultou em duas categorias temáticas: Gestão do Cuidado e Práticas de Cuidado. A primeira é composta por dois meios de trabalho: coordenação de equipe e reuniões com os demais componentes do território. A segunda, envolve três meios de trabalho: contatos telefônicos com serviços da RAPS; encaminhamentos do usuário na RAPS e apoio matricial. Considerações finais: Tendo em vista a superação do modelo manicomial, os meios de trabalho dos enfermeiros são instrumentos de produção de diálogos que visam ampliar o cuidado psicossocial.

Abstract Objectives: The aim of this study was to assess the effect of Myracrodruon urundeuva All. and Qualea grandiflora Mart. leaves hydroalcoholic extracts on viability and metabolism of a microcosm biofilm and on enamel demineralization prevention. Methodology: Microcosm biofilm was produced on bovine enamel using inoculum from pooled human saliva mixed with McBain saliva, under 0.2% sucrose exposure, for 14 days. The biofilm was daily-treated with the extracts for 1 min. At the end, it was analyzed with respect to viability by fluorescence, CFU counting and extracellular polysaccharides (phenol-sulphuric acid colorimetric assay) and lactic acid (enzymatic assay) production. The demineralization was measured by TMR. The data were compared using ANOVA or Kruskal-Wallis (p<0.05). Results: M. urundeuva All. at 100, 10 and 0.1 μg/mL and Q. grandiflora Mart. at 100 and 0.1 μg/mL reduced biofilm viability similarly to positive control (chlorhexidine) and significantly more than the negative-vehicle control (35% ethanol). M. urundeuva at 1000, 100 and 0.1 μg/mL were able to reduce both lactobacilli and mutans streptococci CFU counting, while Q. grandiflora (1000 and 1.0 μg/mL) significantly reduced mutans streptococci CFU counting. On the other hand, the natural extracts were unable to significantly reduce extracellular polysaccharides and lactic acid productions neither the development of enamel carious lesions. Conclusions: The extracts showed antimicrobial properties on microcosm biofilm, however, they had no effect on biofilm metabolism and caries protection.

Abstract Microcosm biofilm has been applied to induce carious lesions in dentin. However, no study has been done to compare the impact of the type of model for providing nutrients to microcosm biofilm formation on dentin. Objective This study compared the performance of two kinds of models (static and semi-dynamic) on the biofilm formation and the development of dentin carious lesions. Material and Methods In both models, biofilm was produced using inoculum from pooled human saliva mixed with McBain saliva for the first 8 h (5% CO2 and 37°C). Afterwards, for the static model, the samples were placed in 24-wells microplate containing McBain saliva with 0.2% sucrose, which was replaced at 24 h. In the semi-dynamic model, the samples were submitted to artificial mouth system with continuous flow of McBain saliva with 0.2% sucrose (0.15 ml/min, 37°C) for 10 h a day (for the other 14 h, no flow was applied, similarly to the static model). After 5 days, biofilm viability was measured by fluorescence and dentin demineralization by transverse microradiography. Results Biofilm viability was significantly lower for the static compared with semi-dynamic model, while dentin demineralization was significantly higher for the first one (p<0.05). The static model was able to produce a higher number of typical subsurface lesions compared with the semi-dynamic model (p<0.05). Conclusions The type of model (static and semi-dynamic) applied in the microcosm biofilm may have influence on it's viability and the severity/profile of dentin carious lesions.

RESUMO Objetivo Investigar potenciais associações entre funções executiva, física global e de alimentação na fase aguda do acidente vascular cerebral (AVC). Métodos Trata-se de estudo transversal envolvendo 63 pacientes admitidos em unidade de AVC de um hospital público. Os critérios de exclusão foram outros diagnósticos neurológicos e/ou psiquiátricos. Os instrumentos utilizados foram: Mini-Exame do Estado Mental e Bateria de Avaliação Frontal para avaliar funções cognitivas; Alberta Stroke Program Early CT Score para quantificação da lesão cerebral; National Institutes of Health Stroke Scale para comprometimento neurológico; Escala Modificada de Rankin para funcionalidade e Functional Oral Intake Scale para função alimentar. Resultados A amostra compreendeu 34 homens e 29 mulheres, sendo a idade média de 63,6 anos. A Bateria de Avaliação Frontal correlacionou significativamente com as demais escalas. Na análise multivariada, a variável independentemente associada com a função executiva foi a Functional Oral Intake Scale. Conclusão A maioria dos pacientes com AVC apresenta alterações das funções executivas que comprometem significativamente a alimentação oral.

ABSTRACT Purpose To investigate potential associations among executive, physical and food functions in the acute phase after stroke. Methods This is a cross-sectional study that evaluated 63 patients admitted to the stroke unit of a public hospital. The exclusion criteria were other neurological and/or psychiatric diagnoses. The tools for evaluation were: Mini-Mental State Examination and Frontal Assessment Battery for cognitive functions; Alberta Stroke Program Early CT Score for quantification of brain injury; National Institutes of Health Stroke Scale for neurological impairment; Modified Rankin Scale for functionality, and the Functional Oral Intake Scale for food function. Results The sample comprised 34 men (54%) and 29 women with a mean age of 63.6 years. The Frontal Assessment Battery was significantly associated with the other scales. In multivariate analysis, executive function was independently associated with the Functional Oral Intake Scale. Conclusion Most patients exhibited executive dysfunction that significantly compromised oral intake.