ABSTRACT Introduction Haploinsufficiency of the hematopoietic transcription factor GATA2 is associated with a broad spectrum of diseases, including infection susceptibility and neoplasms. We aimed to investigate GATA2 variants in patients with non-tuberculous mycobacterial (NTM) and/or fungal infections (FI) without known immunodeficiencies. Method We performed GATA2 genotyping in patients with NTM and/or FI. Results Twenty-two patients were enrolled (seventeen FI, four NTM and one with both infections). The pathogenic variant NG_029334.1:g.16287C>T was found in one patient (4.5%) and two asymptomatic offsprings. We also found the likely-benign variant NG_029334.1:g.12080G>A (rs2335052), the benign variant NG_029334.1:g.16225C>T (rs11708606) and the variant of uncertain significance NG_029334.1:g.16201G>A (rs369850507) in 18.2%, 27.3%, and 4.5% of the cases, respectively. Malignant diseases were additionally diagnosed in six patients. Conclusion Although detected in 45.4% of the patients, most GATA2 variants were benign or likely benign. Identifying a pathogenic variant was essential for driving both the patient's treatment and familial counseling. Pathogenic variants carriers should receive genetic counseling, subsequent infection prevention measures and malignancies surveillance. Additionally, case-control genotyping should be carried out in Brazil to investigate whether the observed variants may be associated with susceptibility to opportunistic infections and/or concurrent neoplasms.

The present work describes the chemical constituents of Odontadenia lutea (Vell.) Markgr. leaves and the toxicities of its extract and fractions against Atta sexdens rubropilosa Forel (Hymenoptera: Formicidae). Chromatographic procedures of the ethanolic extract resulted in the identification of the triterpene β-amyrin, the flavonoid rutin, two fatty acids palmitic and linolenic, and one glyceroglycolipid 3-O-(9,12,15-octadecatrienoyl)-glyceryl-β-D-galactopyranoside, which are known compounds, but they are described for the first time in the Odontadenia genus. The known triterpenes lupeol and α-amyrin were also identified. Structural identification of the compounds was performed by analysis of IR, ESI-MS, and 1D and 2D NMR spectra. The toxicity of its extract and fractions from O. lutea leaves was tested against leaf-cutting ants Atta sexdens rubropilosa Forel by employing ingestion bioassay procedures. The hexane fraction (2 mg mL-1) decreases the average survival of ants from sixteen to six days, causing 98% mortality on the 14th day and 100% at the end of the experiment.

A soja é a principal cultura de verão do Brasil, atualmente a implantação de segunda safra vem sendo crescente. Neste sentido, o conhecimento da contribuição dos caracteres ao rendimento de grãos nestas condições é necessário. O delineamento experimental utilizado foi blocos casualizados, em esquema fatorial 2 x 8, sendo dois locais de cultivo (Salvador das Missões - RS e Tenente Portela - RS) e oito genótipos de soja com hábito de crescimento indeterminado (BMX Elite, NS 5959, NS 5909, BMX Alvo, BMX Potência, BMX Tornado, BMX Turbo, BMX Garra). Os dados foram submetidos a análise de variância pelo teste F (p<0,05), posteriormente, a análise de trilha foi realizada a partir da matriz de correlações, considerando massa total de grãos como variável dependente, e os demais caracteres agronômicos como explicativos. Os componentes de rendimento, o número de legumes com três grãos, o peso de mil grãos dos legumes com três grãos apresentou tendências lineares mais fortes e positivas com o rendimento dos grãos. Os genótipos NS5959, BMX Potência e BMX Turbo obtiveram a maior massa total de grãos, com a maior contribuição do peso de mil grãos dos legumes com dois e três grãos.

The soybean is the main summer crop in Brazil, currently the cultivation of the second crop has been growing. In this sense, the acknowledgement of traits contribution to grain yield in front of these conditions is necessary. The experimental design was in a randomized block, in 2 x 8 factorial scheme, two locals (Salvador das Missões - RS and Tenente Portela - RS) and eight soybean genotypes with indeterminate habit (BMX Elite, NS 5959, NS 5909, BMX Alvo, BMX Potência, BMX Tornado, BMX Turbo, BMX Garra). Data were submited to analysis of variance by the F test (p <0.05), and after the path analysis was performed from the correlation matrix, considering total grain mass as the dependent variable, and the other agronomic traits as explanatory variables. The grain yield components, number of pods with three grains, thousand grain weight of pods with three grains showed stronger and more positive linear trends with grain yield. The genotypes NS5959, BMX Potência and BMX Turbo obtained the highest total grain mass, and that had more contribution of the thousand grain weight of pods with two and three grains.

O objetivo do presente estudo foi traduzir e adaptar, para o português, o BRIEF. O processo de tradução e adaptação seguiu os passos recomendados na literatura internacional. Além disso, foi realizada uma análise inicial das propriedades psicométricas. A versão final foi administrada numa amostra constituída por 277 pais e 282 professores de crianças entre 5 e 11 anos de idade, 112 adolescentes entre 11 e 18 anos da Cidade do Rio de Janeiro. Os valores dos Alfas de Cronbach oscilaram entre 0,901 e 0,957, considerando as 3 formas. A análise de componentes principais identificou duas dimensões. Os valores de adequada consistência interna encontrados na versão brasileira se assemelham aos da versão original, assegurando boa qualidade do processo de tradução e adaptação.

The aim of this study was to translate and adapt the BRIEF into Portuguese. The process of translation and adaptation followed the steps recommended in the international literature. Besides that, an initial analysis of psychometric properties was performed. The final version was administered in a sample of 277 parents, 282 teachers of children between 5 and 11 years old, and 112 adolescents between 11 and 18 years old from the city of Rio de Janeiro. The Cronbach Alphas ranged between .901 and .957 considering the three forms. The main component analysis extracted two dimensions. The internal consistency values found in the Brazilian version resembled the original version, ensuring good quality in the process of translation and adaptation.

OBJETIVO: Reproduzir um novo modelo de defeito ósseo alveolar em ratos Wistar que será utilizado para terapia genética e estudos com células tronco. Adicionalmente, outro objetivo do presente estudo foi determinar o pico de regeneração óssea do defeito criado na região alveolar do modelo experimental. MÉTODOS: Os animais foram aleatoriamente divididos em dois grupos de sete animais. Através de uma incisão gengivobucal foi criado um defeito ósseo medindo 28 mm² de área na região alveolar dos ratos. Os ratos foram sacrificados após duas semanas (n=7) e quatro semanas (n=7) da cirurgia. RESULTADOS: A área média do defeito alveolar após duas semanas de cirurgia foi de 22.27 ± 1.31 mm² e a área média do defeito alveolar após quatro semanas de cirurgia foi de 9.03 ± 1.17 mm². A taxa de formação óssea foi de 5.73 ± 1.31 mm² após duas semanas de cirurgia e de 19 ± 1.17 mm² após quatro semanas de cirurgia. Foi observada diferença estatisticamente significante na taxa de formação óssea entre o grupo dos animais sacrificados com duas e quatro semanas (p=0.003). CONCLUSÃO: Este estudo demonstrou que a maior taxa de regeneração óssea ocorreu no período entre duas e quatro semanas após a cirurgia de criação do defeito ósseo alveolar, portanto esta observação sugere que o período de tempo de quatro semanas será suficiente para avaliar a capacidade de células tronco em regenerar osso em ratos Wistar com defeito ósseo alveolar.

PURPOSE: To study were to reproduce an alveolar bone defect model in Wistar rats to be used for testing the efficacy of stem cell therapies. Additionally, we also aimed to determine the osteogenesis process of this osseous defect in the 1 month period post-surgery. METHODS: The animals were randomly divided into two groups of 7 animals each. A gingivobuccal incision was made, and a bone defect of 28 mm² of area was performed in the alveolar region. Animals were killed at 2 weeks after surgery (n=7) and 4 weeks after surgery (n=7). RESULTS: The average area of the alveolar defect at time point of 2 weeks was 22.27 ± 1.31 mm² and the average area of alveolar defect at time point of 4 weeks was 9.03 ± 1.17 mm². The average amount of bone formation at time point of 2 weeks was 5.73 ± 1.31 mm² and the average amount of bone formation at time point of 4 weeks was 19 ± 1.17 mm². Statistically significant differences between the amount of bone formation at 2 weeks and 4 weeks after surgery were seen (p=0.003). CONCLUSION: The highest rate of ossification occurred mostly from 2 to 4 weeks after surgery. This observation suggests that 4 weeks after the bone defect creation should be a satisfactory timing to assess the potential of bone inductive stem cells to accelerate bone regeneration in Wistar rats.