Abstract The study evaluated the ecology of soil invertebrate faunal communities in periodically flooded forest (CF) fragments and anthropic fields (AF) in the Atlantic Forest. The sampling occurred in the rainy and dry seasons using pitfall traps. We estimated the total activity, richness, diversity, and evenness as well as the activity of the taxonomic and functional groups. Total activity and richness varied as a function of seasonality. Entomobryomorpha, Poduromorpha, and Symphypleona were the most representative taxonomic groups. Enchytraeidae and Blattaria were exclusive to CF. Auchenorrhyncha, Chilopoda, Heteroptera, and Thysanoptera were exclusive to AF. The functional group of microphagous/saprophagous (M/S) showed the highest activity, independent of the environment and season. Greater dissimilarity was observed among the invertebrate fauna in AF when compared to CF. Periodically flooded areas favored groups from the saprophagous trophic guild, while AF areas favored predator and herbivore groups. Soil fauna present in forest environments presented less seasonal variability.

Abstract - Growth Regulating Factors (GRFs) comprise a transcription factor family with important functions in plant growth and development. They are characterized by the presence of QLQ and WRC domains, responsible for interaction with proteins and DNA, respectively. The QLQ domain is named due to the similarity to a protein interaction domain found in the SWI2/SNF2 chromatin remodeling complex. Despite the occurrence of the QLQ domain in both families, the divergence between them had not been further explored. Here, we show evidence for GRF origin and determined its diversification in angiosperm species. Phylogenetic analysis revealed 11 well-supported groups of GRFs in flowering plants. These groups were supported by gene structure, synteny, and protein domain composition. Synteny and phylogenetic analyses allowed us to propose different sets of probable orthologs in the groups. Besides, our results, together with functional data previously published, allowed us to suggest candidate genes for engineering agronomic traits. In addition, we propose that the QLQ domain of GRF genes evolved from the eukaryotic SNF2 QLQ domain, most likely by a duplication event in the common ancestor of the Charophytes and land plants. Altogether, our results are important for advancing the origin and evolution of the GRF family in Streptophyta.

RESUMO. O objetivo do experimento foi avaliar a composição nutricional, a recuperação de matéria seca (MS) e a estabilidade aeróbia de silagens de cereais de inverno submetidas a diferentes tempos de estocagem. Os materiais utilizados foram o trigo (Triticum aestivum cv. BRS Gralha Azul), cevada (Hordeum vulgare cv. BRS Brau), aveia branca (Avena sativa cv. URS Guará), aveia preta (Avena strigosa cv. Embrapa 139) e triticale (X Triticosecale cv. IPR 11), em três tempos de estocagem: 60, 120 e 180 dias. O delineamento experimental foi inteiramente casualizado em arranjo fatorial 5x3. Na avaliação nutricional, a silagem de cevada apresentou baixos teores de fibra em detergente ácido (FDA; 331; 355 e 378 g kg MS-1, para 60, 120 e 180 dias, respectivamente) e altos de nutrientes digestíveis totais (NDT; 558; 544 e 531 g kg MS-1 para 60, 120 e 180 dias, respectivamente), além de alta recuperação de MS e estabilidade aeróbia. O trigo e o triticale apresentaram decréscimo na recuperação de MS com o aumento dos dias de estocagem, embora tenham apresentado alta estabilidade aeróbia. O tempo de estocagem influenciou de maneira distinta as forrageiras, no entanto, tempos de estocagem acima de 60 dias não proporcionaram benefícios para as variáveis avaliadas.

ABSTRACT. The objective of the experiment was to evaluate the nutritional composition, dry matter (DM) recovery and aerobic stability of winter cereal silages under different storage periods. The materials used were wheat (Triticum aestivum cv. BRS Gralha Azul), barley (Hordeum vulgare cv. BRS Brau), white oat (Avena sativa cv. URS Guará), black oat (Avena strigosa cv. Embrapa 139) and triticale (X Triticosecale IPR 11), in three storage periods: 60, 120 and 180 days. The experiment was conducted in a 5x3 factorial, completely randomized design, consisting of five forage species and three storage periods, with five replicates. In nutritional assessment, barley silage presented low values of acid detergent fiber (ADF; 331.2, 355.2 and 378.5 g kg DM-1 for 60, 120, 180 days, respectively), high total digestible nutrients (TDN; 558.2, 544.7 and 531.6 g kg DM-1 for 60, 120, 180 days, respectively), high DM recovery and aerobic stability. Wheat and triticale showed a decrease in DM recovery with the increase in storage length, although showed high aerobic stability. The storage period had a different effect on forages; however, storage period above 60 days provided no benefits for the variables evaluated.

Soybean [Glycine max (L.) Merril], one of the most important crop species in the world, is very susceptible to abiotic and biotic stress. Soybean plants have developed a variety of molecular mechanisms that help them survive stressful conditions. Hybrid proline-rich proteins (HyPRPs) constitute a family of cell-wall proteins with a variable N-terminal domain and conserved C-terminal domain that is phylogenetically related to non-specific lipid transfer proteins. Members of the HyPRP family are involved in basic cellular processes and their expression and activity are modulated by environmental factors. In this study, microarray analysis and real time RT-qPCR were used to identify putative HyPRP genes in the soybean genome and to assess their expression in different plant tissues. Some of the genes were also analyzed by time-course real time RT-qPCR in response to infection by Phakopsora pachyrhizi, the causal agent of Asian soybean rust disease. Our findings indicate that the time of induction of a defense pathway is crucial in triggering the soybean resistance response to P. pachyrhizi. This is the first study to identify the soybean HyPRP group B family and to analyze disease-responsive GmHyPRP during infection by P. pachyrhizi.

A tolerância ao frio nos estádios iniciais de desenvolvimento do arroz (Oryza sativa L.) é uma característica altamente desejável para incorporar em cultivares do Rio Grande do Sul (RS), porém a seleção para esta característica deve ser feita sob condições de temperatura controlada, o que limita o número de linhas que pode ser avaliada. O conhecimento da herança desta característica é importante para definir estratégias de melhoramento genético, assim o objetivo do presente trabalho é estudar a base genética da tolerância ao frio no estádio vegetativo do arroz. Seis genótipos com reações contrastantes de tolerância ao frio foram cruzados num esquema dialélico sem os recíprocos. Os genitores e as gerações F1 e F2 foram cultivados em casa-de-vegetação até o estádio V4, quando foram submetidos a 10°C por dez dias e avaliados quanto à sobrevivência de plantas após sete dias de recuperação sob temperatura normal. Os resultados obtidos pela análise dialélica da geração F1 indicaram significância tanto dos efeitos aditivos quanto não-aditivos, mas a capacidade geral de combinação foi mais importante. A avaliação da geração F2 revelou uma herança oligogênica com um ou dois alelos dominantes responsáveis pela tolerância ao frio nos genitores tolerantes e dois genes complementares com alelos recessivos segregando nos cruzamentos envolvendo genótipos sensíveis e intermediários.

Rice (Oryza sativa L.) cold tolerance at the initial stages of development is a highly desirable trait to be incorporated into the state of Rio Grande do Sul, Brazil, cultivars, but selection for this trait must be performed under controlled temperature conditions, which limits the number of lines that can be evaluated. Knowledge of the inheritance of this trait is important to define breeding strategies. So the aim of this paper was to study the genetic basis of rice cold tolerance at the vegetative stage. Six genotypes with constrasting cold tolerance reactions were crossed in a diallel scheme without the reciprocals. The parents and the F1 and F2 generations were cultivated in a greenhouse until the V4 stage, when they were submitted to 10°C for ten days and evaluated for plant survival after seven days of recovery under normal temperature. The results obtained by the diallel analysis of the F1 generation indicated significance of both additive and non-additive effects, but the general combining ability was more important. The evaluation of the F2 generation revealed oligogenic inheritance with one or two dominant alleles responsible for cold tolerance in the cold tolerant parents and two complementary genes with recessive alleles segregating in the crosses involving sensitive and intermediate genotypes.

Rice is a tropical plant, so cold temperature may be detrimental to its development, depending on the genotype and environmental conditions. Degree of lipid unsaturation has been related to cold tolerance due to its effect on membrane stability. So, the aim of this study was to characterize the fatty acid composition and its alterations due to cold temperature in rice genotypes of diversified origin. Forty-four rice genotypes at the V4 stage were submitted to two temperature conditions: 10°C and 28°C for two days and after this they had their leaves collected for lipid extraction and quantification. Control plants were allowed to regrow until presenting four leaves fully expanded and then were subjected to 10°C for ten days for cold tolerance evaluation. Plant survival was measured seven days after recovery at 28°C and the genotypes were grouped in three cold tolerance classes: tolerant, intermediate and sensitive. These classes differed for total saturated and unsaturated fatty acids only under the cold temperature treatment. Further analysis of the more abundant fatty acids: linoleic, linolenic and palmitic, showed that the two last ones differed between tolerant and sensitive genotypes. Linolenic acid increased after cold exposure in cold tolerant genotypes while palmitic acid decreased, and an opposite behavior was found in the cold sensitive genotypes. These evidences indicate that these fatty acids are potential molecular markers useful for breeding programs as well as for future basic studies on cold tolerance in rice.