Two magnetotelluric (MT) profiles perpendicular to the trench provide information on the subduction of the Rivera plate under the Jalisco block (JB). The geometry of the subducting slab is inferred by the anomalous conductor on the top of the profile in the central part of the JB. High conductivity zones (<50 ohm-m) at depths shallower than 10 km are associated to dewatering of the oceanic crust below the accretion prism in the SW of the profile. Away from the coast, observed upper crustal conductors (<10 km) are interpreted as partial melt related to the Central Jalisco volcanic lineament. The source of the crustal conductor in the central part of the MT profile, ~25-75 km inland and depths between 40 and 60 km, is interpreted as a region of interconnected fluids associated with the metamorphic dehydration of the oceanic plate. Contrasting resistivity in the mantle wedge at depths below 40 km suggests that hot mantle material may be migrating upwards, mixing with dehydration reaction products. Near Bahla de Banderas subduction appears steeper closer to the trench than toward the SW suggesting a twisted oceanic subducting slab beneath the continental crustal block. Our results support the mantle upwelling model as an explanation for the reported 1.5 km uplift of the central part of the JB and subsidence of the fore arc region, as well as a change in mantle properties at the NW edge of the JB, which is backed by seismic data.

Dos perfiles magnetotelúricos (MT) perpendiculares a la trinchera proporcionan información del proceso de subducción de la placa de Rivera debajo del bloque de Jalisco (BJ). A partir del conductor anómalo inclinado que se asocia a la interface activa se deducen rasgos de la geometría de la placa que subduce. Zonas de conductividad anómala (<50 ohm-m) a profundidades inferiores a los 10 km se asocian a la deshidratación de la corteza oceánica debajo del prisma de acreción en el SW del perfil. Lejos de la costa, los conductores observados a profundidades de la corteza superior (<10 km) se interpretan como zonas de fusión parcial relacionadas al lineamiento volcánico de Jalisco Central. La fuente de la zona conductora cortical en la parte central del perfil MT, aproximadamente 25 a 75 km dentro del continente y entre los 40 y 60 km de profundidad, se interpreta como una región de fluidos interconectados asociados con la deshidratación de la corteza oceánica por procesos de metamorfismo. El contraste de resistividades en la cuña del manto debajo de ~40 km sugiere la migración ascendente de material caliente del manto que se mezcla con productos de reacciones metamórficas de la placa oceánica. El proceso de subducción de la placa de Rivera ocurre a un ángulo más pronunciado y más cercano a la trinchera en la parte noroeste que hacia la parte sureste de la zona de subducción, lo que sugiere una placa oceánica flexionada hacia el NW debajo del bloque de corteza continental. Nuestros resultados apoyan un modelo de ascenso del manto en la parte centro oriental del BJ, que explica el levantamiento topográfico de más de 1.5 km y la subsidencia en la zona del ante-arco reportada, además de un cambio en las propiedades eléctricas del manto en la zona, que parecen variar rápidamente, como lo sugieren también los datos sísmicos.

Canine visceral leishmaniasis (CVL) is endemic in numerous Brazilian regions. The greatest difficulty in controlling the disease is the diagnostic limitation. In the present study, the most common tests employed for visceral leishmaniasis diagnosis were compared: immunofluorescence antibody test (IFAT), immunoenzymatic assay (ELISA), direct parasitological examination and polymerase chain reaction (PCR). Samples of lymph node aspirates and blood were collected from 100 dogs that lived in an endemic area (Bauru city, São Paulo state) and from 100 negative controls from a non-endemic area (Botucatu city, São Paulo state). Specificity of both IFAT and PCR was 100% whereas ELISA was 99%. Sensitivities were 97.77, 93.33 and 91.11% respectively for IFAT, ELISA and PCR.

Pesquisou-se Neospora caninum como causador de abortamento e natimortalidade em bovinos, por meio de exame histopatológico (hematoxilina-eosina), imunoistoquímica (IHQ) e nested-PCR, utilizando primers da região Nc5 do DNA genômico (PCR PLUS) e primers da região ITS1 do DNA ribossomal (PCR JB). Foram avaliadas 105 amostras de abortamento bovino entre janeiro de 2006 a maio de 2008, encaminhadas ao Centro de Pesquisa e Desenvolvimento de Sanidade Animal do Instituto Biológico para diagnóstico diferencial de causas infecciosas. Observou-se em 71,4% das amostras lesões histológicas (HE) caracterizadas pela presença de células inflamatórias mononucleares no coração, pulmão, fígado, rim, placenta e cérebro. A IHQ detectou 8,57% de positividade, sendo maior no cérebro, placenta e coração. A nested-PCR JB revelou 6,66% de casos positivos, enquanto que a nested-PCR PLUS apresentou maior taxa de positividade (20,95%), principalmente no cérebro, placenta e no pool fígado/coração. Houve baixo grau de concordância entre as três técnicas pelo índice kappa. A taxa de positividade para qualquer uma das técnicas (IHQ, nested-PCR PLUS e nested-PCR JB) foi de 24,8%, devendo ser consideradas conjuntamente pelo fato de possuírem características distintas e serem, portanto, complementares no diagnóstico definitivo da neosporose.

Neospora caninum, a cause of abortion and stillbirth in cattle, was studied by histology, immunohistochemistry, and nested-PCR, using primers from the Nc5 region of the genomic DNA (PCR PLUS) and primers from the ITS1 region of the ribosomal DNA (PCR JB). A total of 105 fetal samples sent to the Centro de Pesquisa e Desenvolvimento de Sanidade Animal do Instituto Biológico from January 2006 to May 2008 were examined for evidence of N. caninum. Histological examination revealed 71.4% with non-suppurative inflammation in the heart, lung, liver, kidney, placenta, and brain. Immunohistochemistry detected infections in 8.6% of the samples, mainly in the brain, placenta, and heart. Nested-PCR JB revealed 6.7% with infections, while nested-PCR PLUS returned 20.9% positive results, mainly in brain and placenta, and in the pooled liver and heart. Kappa statistics demonstrated little agreement among the three techniques. The three methods are complementary, since they have distinct diagnostic characteristics and were combined to give a positivity rate of 24.8%.