ABSTRACT The need to use strategies for using brackish water in agriculture, especially in semi-arid conditions, is evident. Based on this information, this study was developed with the aim of evaluating the consumption, efficiency and water content, as well as the dry mass of the arugula plants (cv. Broad Leaf) exposed to brackish nutrient solutions as a function of replacement strategies and circulation frequencies. The treatments consisted of six salinity levels of the nutrient solution (1.5, 3.0, 4.5, 6.0, 7.5 and 9.0 dS m-1) and two circulation frequencies (twice a day 8 a.m. and 4 p.m., and three times a day at 8 a.m., 12 p.m. and 5 p.m.). In Experiment I, the evapotranspiration line was replaced with the respective brackish water used in the preparation of the solution, and in Experiment II, the water supply was used. In both treatments, the experimental design was completely randomized, in a 6 x 2 factorial scheme, with five replications. The conclusion is that it is technically feasible to circulate the nutrient solution twice a day and there were lower losses in the water relations, in the biomass production of the shoot and in the partition of photoassimilates when the replacement with water supply was adopted.

Abstract This work aimed to evaluate the production of lipolytic complexes, produced by microorganisms isolated from a biological treatment system of effluents from a hotel. To select the best lipolytic microorganism for use in biotechnological processes, we tested 45 bacterial isolates recovered from the raw effluent of the hotel’s restaurant waste tank. Lipase production was assayed in culture medium supplemented with olive oil and rhodamine B, incubated at 25 °C and 30 °C for 24 h - 48 h. Results showed 22 isolates lipase producers. All isolates were inoculated on medium without yeast extract to select the ones with highest enzyme yields. Out of these, nine isolates showed high lipase activity. The strain with the larger halo was assayed in submerged culture using an orbital shaker and a bioreactor, with three different substrates (olive oil, grape seed oil, and canola oil). Isolate G40 identified as Acinetobacter baylyi was selected to run the production assays because it showed the best result in the solid medium. In the bioreactor, maximum lipase production was obtained after 12 h of culture with the three substrates evaluated: 0,358 U/mL.min-1 in olive oil, 0,352 U/mL.min-1 with grapeseed oil, and 0,348 U/mL.min-1 with canola oil.