Oropouche virus (OROV) is an arthropod-borne virus of the Peribunyaviridae family, transmitted to humans primarily by Culicoides paraensis. It is one of the main arboviruses infecting humans in Brazil, primarily in the Amazon Region. Here, we report the detection of OROV in the saliva and urine of a patient whose samples were collected five days after the onset of symptoms. Nucleotide sequencing and phylogenetic analysis further confirmed the results. To our knowledge, this is the first study reporting the detection of OROV in the saliva and urine of an infected patient. In addition, the results of our study expand the current knowledge pertaining to the natural history of Oropouche fever.

Abstract Purpose: To determine the efficacy of norbixin-based poly(hydroxybutyrate) (PHB) membranes for Achilles tendon repair. Methods: Thirty rats were submitted to total tenotomy surgery of the right Achilles tendon and divided into two groups (control and membrane; n = 15 each), which were further subdivided into three subgroups (days 7, 14, and 21; n = 5 each). Samples were analyzed histologically. Results: Histological analysis showed a significant reduction in inflammatory infiltrates on days 7, 14 (p < 0.0001 for both), and 21 (p = 0.0004) in the membrane group compared to that in the control group. There was also a significant decrease in the number of fibroblasts in the control group on days 7, 14 (p < 0.0001), and 21 (p = 0.0032). Further, an increase in type I collagen deposition was observed in the membrane group compared to that in the control group on days 7 (p = 0.0133) and 14 (p = 0.0107). Conclusion: Treatment with norbixin-based PHB membranes reduces the inflammatory response, increases fibroblast proliferation, and improves collagen production in the tendon repair region, especially between days 7 and 14.

Conhecer como os caracteres agronômicos se correlacionam nas populações segregantes é primordial ao melhorista de plantas, principalmente quando o objetivo for seleção indireta, por meio de um caráter de fácil mensuração ou de maior herdabilidade correlacionado ao caráter de interesse de menor herdabilidade. Dessa forma, a partir da variabilidade observada em caracteres agronômicos nas gerações F2 e F3 de quatro cruzamentos de trigo, avaliados no ano agrícola de 2004, no Centro Agropecuário da Palma, do Centro de Genômica e Fitomelhoramento - FAEM/UFPel, localizado no município de Capão do Leão (RS), este trabalho teve como objetivos estimar: a) coeficientes de correlação genotípica, fenotípica e de ambiente entre os caracteres; b) coeficientes de trilha sobre o rendimento de grãos. Em todas as populações avaliadas, por meio das correlações simples, a seleção de plantas mais produtivas pode ser realizada pela seleção indireta sobre número de afilhos férteis por planta. Mediante a análise de trilha foi possível identificar o efeito indireto do caráter número de afilhos férteis como causador da formação da correlação entre outros caracteres (comprimento da espiga x rendimento de grãos, número de espiguetas da espiga x rendimento de grãos e número de grãos da espiga x rendimento de grãos), enfatizando que o número de afilhos férteis é o principal caráter a ser considerado na obtenção de ganhos em produção por planta.

The knowledge of how the agronomical traits are correlated in segregating populations is of primary importance for plant breeders, specially when one considers indirect selection through a character easily measurable or with high heritability correlated to the trait of interest with lower heritability. Agronomical traits in F2 and F3 generations of four wheat crosses, were evaluated on 2004 agricultural year in the Centro Agropecuário da Palma, of the Centro de Genômica e Fitomelhoramento - FAEM/UFPel, located in Capão do Leão country in Rio Grande do Sul State. The present work had the objectives to estimate for evaluated traits: a) genotypic, phenotypic and environmental correlation coefficients between the traits; and b) path coefficients for grain yield. For all populations, the single correlations indicated that the selection of higher yielding plants can be performed by indirect selection for number of fertile tillers per plant. The path analysis showed that it was possible to identify the indirect effect of the trait number of fertile tillers the as cause of the correlation between other traits (ear length x grain yield, spikelet number per ear x grain yield and number of ear grain x grain yield). Thus suggesting that the number of fertile tillers is the major trait to select for in order to obtain gains on grain yield.