Resumen El almidón resistente (AR) es una fracción del almidón no digerible por las α-amilasas y a diferencia de la fibra dietética, el AR es fermentado en el colon promoviendo beneficios en la salud. Dentro de los diferentes tipos de AR, el tipo 3 (AR3) se obtiene por autoclaveado y sin el uso de agentes químicos. El objetivo de este estudio fue obtener AR3 a partir de avena y evaluar su efecto a diferentes concentraciones (0, 5, 10, 15 y 20 %, p/p) sobre las propiedades reológicas de la masa, propiedades de textura de las galletas y su aceptabilidad sensorial. El análisis de perfil de textura (TPA) evidenció un incremento en la fracturabilidad, dureza, adhesividad y gomosidad. La incorporación de AR3 incrementó el módulo elástico (G´) y viscoso (G´´) en comparación con la masa control. Todos los tratamientos presentaron comportamiento elástico (G´ > G´´). La dureza en las galletas aumentó con la concentración de AR3, con valores de ≈ 8 N (control) hasta 20 N (AR3-20 %). Las pruebas sensoriales no fueron significativas entre tratamientos, aunque las galletas AR3-20 % tuvieron mayor preferencia. La adición de AR3 en la masa provocó cambios funcionales y de textura significativos que afectaron positivamente los atributos de calidad de las galletas.

Abstract Resistant starch (RS) is a portion of starch that is not digestible by α-amylases and unlike dietary fiber, RS is fermented in the colon, promoting health benefits. Among the different types of RS, type 3 (RS3) is obtained by autoclaving and without chemical agents. The objective of this work was to obtain RS3 from oats and to evaluate its effect at different concentrations (0, 5, 10, 15 and 20 % w/w) on the rheological properties of the dough, textural properties of the cookies and their sensory acceptability. Texture profile analysis (TPA) showed an increased fracturability, hardness, adhesiveness and gumminess. The incorporation of RS3 increased the elastic (G’) and viscous (G’’) modulus compared to the control dough. All treatments showed elastic behavior (G’ > G’’). Hardness in cookies increased with RS3 concentration, with values from ≈ 8 N (control) to 20 N (RS3 - 20 %). Sensory tests were not significant between treatments, although RS3-20 % cookies had a higher preference. The addition of RS3 in the dough caused significant functional and textural changes that positively affected the quality attributes of the cookies.

La producción del papel en Venezuela genera una gran cantidad de desechos de fibras de celulosa no utilizables (lodo papelero), produciéndose problemas ambientales y de almacenamiento. La búsqueda de usos para este lodo papelero disminuiría los problemas generados. En este trabajo, se utilizó al hongo Trichoderma reesei para la realización de fermentaciones líquidas y el estudio del efecto de sales minerales, tamaño de partícula del lodo y pretratamientos ácidos y básicos, sobre la producción de azúcares reductores y la actividad celulasa. También se realizaron fermentaciones sólidas para obtener biomasa (esporas) del hongo. Los resultados indicaron que el uso de lodo higiénico, sales minerales y la reducción del tamaño de partícula, mejoran el rendimiento en azúcares reductores (AR). La máxima actividad celulasa obtenida, medida con carboximetilcelulosa, fue de 2,6 mg AR/L/h. Se determinó que con el pretratamiento ácido se obtienen mayores cantidades de AR (17 mg/L) que con el básico (7,4 mg/L). Se demostró que T. reesei puede crecer en fermentaciones sólidas a expensas del lodo papelero generando hasta 1,24x10(8) esporas por gramo de lodo seco. Se requiere estandarizar y escalar estas técnicas para usar al lodo papelero como materia prima en este tipo de procesos industriales.

Paper production in Venezuela generates large amounts of cellulose fibers (paper sludge), producing environmental and storage problems. Finding uses for this papermaking sludge would decrease the problems caused. In this work the fungus Trichoderma reesei was used on submerged fermentation to study the effect of mineral salts, particle size and acid-alkali pretreatment of sludge on production of reducing sugars (RS) and cellulase activity. Solid fermentations were also conducted to obtain biomass (spores) of the fungus. The results indicated that the use of sanitized sludge, mineral salts and smaller particles, increased the production of reducing sugars (RS). The maximum cellulase activity, measured with carboxymethylcellulose, was RS 2.6 mg/L/h. Acid pretreatment was the best in rendering RS (17 mg/L) in comparison with alkali treatment (7.4 mg/L). It was also demonstrated that T. reesei can grow on paper sludge producing biomass (spores) with a yield of 1,24x10(8) spores/g of dry sludge. It is necessary to standardize and scale range these procedures in order to be able to use paper sludge to industrialize these processes.

No presente estudo empregou-se o método de proteômica para obter a expressão diferencial de proteínas em hipocampo de pacientes com epilepsia do lobo temporal (ELT) (N=6) comparado a hipocampos controle obtidos por meio de autópsia (N=6). O estudo foi feito por meio de eletroforese bidimensional, acoplada a HPLC e espectroscopia de massa. As proteínas que tiveram expressão aumentada no hipocampo de pacientes com ELT foram: isoforma-1 da soroalbumina, HSP70, diidropirimidinase-2, isoforma-1 da proteína básica da mielina, subunidade catalítica A da próton ATPase, glutationa S-transferase P, proteína DJ-1 (PARK7), e resíduo diidropolilisina do componente acetil-transferase do complexo da piruvato desidrogenase. A expressão da isoforma-3 da cadeia alfa da espectrina (fodrina) foi menor no hipocampo de pacientes com epilepsia do lobo temporal e as proteínas glutationa S-transferase P e PARK7 foram detectadas somente no tecido epiléptico. Assim, nossos resultados trazem evidencias sobre a direta relação entre distúrbio metabólico e dano oxidativo na patofisiologia da ELT. Além disto, o estudo indica biomarcadores para futuras investigações como alvos terapêuticos para epilepsia.

In this study we used proteomics approaches to obtain the protein profile of human epileptic hippocampi (N=6) and control hippocampi obtained from autopsy (N=6). We used two-dimensional gel electrophoresis (2-D) coupled to HPLC and Mass spectroscopy (MALDI-TOF) to identify proteins differentially expressed. Nine proteins were differentially expressed comparing the hippocampus of patients with the hippocampus of control. Proteins that were increased in the hippocampus of patients with TLE were: isoform 1 of serum albumin, HSP 70, dihydropyrimidinase-related protein 2, isoform 1 of myelin basic protein, proton ATPase catalytic subunit A, and dihydrolipoyllysine-residue acethyltransferase component of pyruvate dehydrogenase complex. The expression of isoform 3 of spectrin alpha chain (fodrin) was down-regulated while the proteins glutathione S-transferase P and the DJ-1 (PARK7) were detected only in the hippocampus of patients with TLE. Taken together, our results provide evidence supporting a direct link between metabolic disturb and oxidative damage related to pathophysiology of TLE. Besides, indicates biomarkers for further investigations as therapies targeted to epilepsy.

A produção de tomates no Estado do RS ocupa um importante papel sócio-econômico, que pode ser constatado pelo crescimento do cultivo dessa hortaliça em ambiente protegido. Essa técnica permite a produção de tomates em período de entressafra; no entanto, ocorrem também condições favoráveis para o desenvolvimento de doenças fúngicas como o mofo cinzento provocado por Botrytis cinerea. O surgimento de raças de patógenos resistentes a fungicidas químicos vem fazendo com que o controle biológico torne-se uma alternativa necessária. Neste trabalho foi realizada seleção in vitro de 24 isolados do fungo Trichoderma harzianum e 12 de Gliocladium viride que inibiram o desenvolvimento do patógeno B. cinerea. Foram selecionados dois isolados (TRIC-30 e GLIO-10) para serem testados em experimentos em condições de campo com tomates cultivados sob ambiente protegido, nos quais a pulverização foliar semanal com uma suspensão com 2x10(7) conídios mL-1 reduziu significativamente a incidência do mofo cinzento, enquanto a aplicação dos antagonistas nas sementes, no substrato e na cova, no momento do plantio, não reduziu a incidência do patógeno.

The production of tomato in the State of Rio Grande do Sul performs an important economical and social role that can be evidenced by the increase in cultivation of this vegetable in protected environment. This practice allows the production of tomato during the off-season periods. However, it can also promote favorable conditions to the development of gray mold caused by Botrytis cinerea, and the arising of pathogen races resistant to fungicides is turning biological control into a necessary alternative. In the present work, an in vitro selection among 24 isolates of the fungus Trichoderma harzianum and 12 of Gliocladium viride that inhibited the development of the pathogen B. cinerea was carried out. Two isolates (TRIC-30 e GLIO-10) were selected to be tested in an experiment in field conditions with tomatoes cultivated under protected environment. In this experiment, the weekly foliar pulverization of a suspension with 2x10(7) conidia mL-1 significantly decreased the incidence of gray mold, while the application of the antagonists on the seeds, substrate and seed hole during seeding did not reduced the incidence of the pathogen.