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ABSTRACT In irrigated production systems, the common bean grain yield must reach high levels to maintain economic viability. In this context, management, like the selection of the most adapted cultivars, may be effective in obtaining high yields. Through a three-year experiment, it was aimed to select the common bean cultivars with the highest agronomic and technological performance in an irrigated production system and to determine the yield component that most interfered with common bean yield. The treatments consisted of eight common bean cultivars with indeterminate growth habit and from the pinto bean variety (carioca): ANFc 9, BRS Estilo, BRSMG Madrepérola, Pérola, IAC Alvorada, IAC Milênio, TAA Bola Cheia, and TAA Dama. IAC Milênio and IAC Alvorada had the highest agronomic performance for irrigated production systems, with grain yields up to 17% higher than other cultivars. The cultivars show little variation in the grain technological quality, meeting the culinary and nutritional qualities recommended for common bean. The ANFc 9 cultivar showed the highest crude protein content in grains (19%). The number of pods per plant is the yield component that most interferes with common bean grain yield, generating 86 kg ha-1 increments for each unitary increase. Therefore, the common bean cultivars selection in the irrigated production system is an effective management practice to increase common bean agronomic and quality performance.

RESUMO Em sistemas de produção irrigados, a produtividade de grãos do feijão-comum deve atingir níveis elevados para manter a viabilidade econômica. Nesse contexto, manejos como a seleção de cultivares mais adaptadas podem ser eficazes na obtenção de altas produtividades. Através de um experimento de três anos, objetivou-se selecionar as cultivares de feijão-comum com maior desempenho agronômico e tecnológico em sistema de produção irrigado, além de determinar o componente de produção que mais interfere na produtividade de grãos. Os tratamentos consistiram de oito cultivares de feijão-comum de hábito de crescimento indeterminado e grupo comercial de grãos ‘carioca’: ANFc 9, BRS Estilo, BRSMG Madrepérola, Pérola, IAC Alvorada, IAC Milênio, TAA Bola Cheia e TAA Dama. As cultivares IAC Milênio e IAC Alvorada apresentam o maior desempenho agronômico para o sistema de produção irrigado, com produtividade de grãos até 17% superior em relação às demais cultivares. As cultivares apresentam pouca variação na qualidade tecnológica do grão, atendendo às qualidades culinárias e nutricionais recomendadas para o feijão-comum. A cultivar ANFc 9 apresentou o maior teor de proteína bruta nos grãos (19%). O número de vagens por planta foi o componente de produção que mais interferiu na produtividade de grãos do feijão-comum, gerando incrementos de 86 kg ha-1 para cada aumento unitário. Portanto, a seleção de cultivares de feijão em sistema de produção irrigado é uma prática de manejo eficaz para aumentar o desempenho agronômico e qualitativo do feijão-comum.

https://doi.org/10.1590/1807-1929/agriambi.v27n11p882-891

Green Synthesis, Characterization and Antibacterial and Leishmanicidal Activities of Silver Nanoparticles Obtained from Aqueous Extract of Eucalyptus grandis
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Oliveira, Lucas M. F.

; Silva, Ueveton P. da

; Braga, João Pedro V.

; Teixeira, Álvaro V. N. C.

; Ribon, Andréa O. B.

; Varejão, Eduardo V. V.

; Coelho, Eduardo A. F.

; Freitas, Camila S. de

; Teixeira, Róbson R.

; Moreira, Renata P L.

Journal of the Brazilian Chemical Society

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Abr 2023, Volume 34 Nº 4 Páginas 527 - 536

Resumo: En | Texto: En | PDF: En

This work describes a green synthesis, the characterization, and biological evaluation of silver nanoparticles (AgNPs). The AgNPs suspension was synthesized using aqueous leaf extract of Eucalyptus grandis, which presented a characteristic band at 407 nm in the UV-Vis spectrum. The AgNPs presented a spherical shape and size of 9.7 ± 0.3 nm. The nanoparticles were stable over a month, indicating that E. grandis’ extract is suitable for their preparation and stabilization. The X-ray analysis showed that the crystallinity of AgNPs corresponded to the centered face phase of silver. The antibacterial and leishmanicidal activities of AgNPs were evaluated. The AgNPs presented antibacterial activity on the Gram-negative bacteria Escherichia coli at 53.9 µg mL-1. The leishmanicidal activity evaluation against promastigote forms of Leishmania infantum, Leishmania amazonensis, and Leishmania braziliensis showed that the biological response is dependent on the volume of AgNP suspension. It was demonstrated that L. infantum was more sensitive to the nanoparticle’s treatment than L. amazonensis and L. braziliensis. The treatment of L infantum promastigotes with 150 µL of AgNP suspension reduced parasite growth by 67.9%, a result which was similar to the treatment with 1 (66.7%) or 2 µL (70.6%) of amphotericin B used as a positive control.

https://doi.org/10.21577/0103-5053.20220126

Assessment of Chlorine, Fluorine, and Sulfur Concentrations in Depth Profile of Drill Cuttings from Onshore and Offshore Oil and Gas Exploration Wells
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Marim, Renan G.

; Coelho Junior, Gilberto S.

; Azevedo, Paola C. C. de

; Borges, Daniel L. G.

; Maranhão, Tatiane A.

; Oliveira, Fernando J. S. de

; Bascunãn, Vera L. A. F.

; Chaves, Eduardo S.

Journal of the Brazilian Chemical Society

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Mar 2023, Volume 34 Nº 3 Páginas 414 - 425

Resumo: En | Texto: En | PDF: En

The assessment of chlorine, fluorine, and sulfur concentrations in depth profile of drill cuttings was performed using ion chromatography after sample preparation by pyrohydrolysis. The parameters for pyrohydrolysis were optimized using a Box-Behnken design, and the established conditions were 300 mg of sample, 300 mg of V2O5, temperature of 1000 °C, reaction time of 25 min, and absorbing solution of 50 mmol L-1 NH3 + 150 mmol L-1 H2O2. The accuracy was evaluated by the analysis of certified reference materials and recovery tests, and the results showed a good agreement between determined and certified values and recoveries (89-108%). The precision (relative standard deviation (RSD) < 10%), and the limits of detection (0.5-2.5 mg kg 1) were suitable for drill cuttings analysis. The concentration of chlorine, fluorine, and sulfur in drill cuttings at different depths was at mg g-1 range, and its variation is related to the mineral composition of the samples.

https://doi.org/10.21577/0103-5053.20220118

Study of the Molecular Weight of Pullulan Produced by Aureobasidium pullulans from Industrial Waste
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Oliveira, Victor R. L.

; Coelho, Victória V.

; Sérvulo, Eliana F. C.

; Calado, Verônica M. de A.

Materials Research

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2023, Volume 26 Supl. 1 elocation e20230060

Resumo: En | Texto: En | PDF: En

The objective of this research was the sustainable development of pullulan and its structural and rheological characterization. In addition, the cultivation of the A. pullulans Y2092 strain by submerged fermentation on a laboratory scale was evaluated, valuing the use of industrial waste (residual brewery's yeast), RBY, and a low-cost renewable raw material VHP (very high polarization sugar). A 22 factorial statistical design of experiments was used to analyze the molecular weight of the pullulans produced. The maximum value of 1955 kDa was determined for the pullulan recovered after 96 h of fermentation in the mineral medium added with 70 g/L of VHP and 50 g/L of RBY. The FTIR analysis for the pullulan obtained was similar to the commercial pullulan. The rheological behavior of pullulan solutions is shown, evidencing the relationship between this property and Mw. The results from all analyses indicate that the pullulan obtained is suitable for the future production of biopolymeric films.

https://doi.org/10.1590/1980-5373-mr-2023-0060

Synthesis and Anticancer Activity of Homodimeric Morita-Baylis-Hillman Adducts Based on 3-Hydroxyindolin-2-one Core
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Coelho, Maísa C.

; Castro, Aleff

; Olegário, Tayná R.

; Cristiano, Rodrigo

; Vaz, Boniek G.

; Santos, Gabriel F. dos

; Machado, Lucas S.

; Militão, Gardênia C. G.

; Silva, Paulo B. N. da

; Vasconcellos, Mário L. A. A.

; Lima-Junior, Claudio G.

Journal of the Brazilian Chemical Society

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2023, Volume 34 Nº 9 Páginas 1273 - 1283

Resumo: En | Texto: En | PDF: En

Cancer treatment represents one of the main scientific study targets today, mainly due to the pronounced side effects arising from chemotherapy. This study reports the synthesis, characterization, and anticancer activity of ten compounds from the Morita-Baylis-Hillman reaction. Ethylene glycol diacrylate was used as a double Michael acceptor in reactions with isatin derivatives to give homodimers of 3-hydroxyindolin-2-one core, recognized in the literature for its extensive pharmacological profile. The use of 1,4-diazabicyclo[2,2,2]octane (DABCO) as a catalyst and room temperature were the optimal conditions for the study reaction. The isolated yields were up to 63%, with most reaction times inferior to 24 h, some as fast as 15 min. The anticancer potential of the synthesized dimers was evaluated in vitro against three cancer strains, resulting in average inhibitory concentrations up to 0.72 µM. It was also found that the best performing homodimers are more active than their monomeric counterparts. Considering the promising selectivity indices observed, the preliminary results obtained here act as a basis for broader tests regarding the effectiveness of homodimeric adducts against cancer cells.

https://doi.org/10.21577/0103-5053.20230038

Herpetofauna of protected areas in the Caatinga VIII: An updated checklist for the Serra das Confusões region with new data from Serra Vermelha, Piauí, Brazil
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Marques, Ricardo

; Garda, Adrian A.

; Furtado, Adriana P.

; Bruinjé, Andre C.

; Protázio, Arielson dos S.

; de Carvalho, Breno F.

; Vieira, Cecília R.

; Gomes, Dandara

; Pantoja, Davi L.

; Figueiredo, Diainara da S.

; Shepard, Donald B.

; Camurugi, Felipe

; Coelho, Felipe E. A.

; Magalhães, Felipe M.

; Caetano, Gabriel H. O.

; Colli, Guarino R.

; Paulino, Henrique M.

; Carvalho, Izabelle T. S.

; Graciene, Joedma

; Alvarenga, Júlio M.

; Clay, Natalie A.

; Albuquerque, Ralph L.

; Bosque, Renan J.

; Faria, Renato

; da Silveira-Filho, Ricardo R.

; Mângia, Sarah

; Cavalcante, Vitor H. G. L.

; Vieira, Washington L. S.

; da Silva, Willianilson P.

; Soares, Yan F. F.

; Mesquita, Daniel O.

Biota Neotropica

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2023, Volume 23 Nº 4 elocation e20231520

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract It is repeatedly stressed the need to characterize the extant biodiversity in tropical ecosystems. However, inventory studies are still progressing slowly in dry ecosystems, leading to the underestimation of their true biodiversity and hindering conservation efforts. In this study, we present primary and secondary data, along with an updated list of amphibians and reptiles from two localities in the São Francisco-Gurguéia region in Piauí. Additionally, we compare the species composition between nine areas within the Caatinga, which were sampled using standardized methods over the past ten years, to examine broader spatial patterns of community composition. To survey reptiles and amphibians, we employed similar methods and sampling efforts in two areas within the Serra das Confusões National Park (SCNP) region. Our surveys recorded a total of 73 species of amphibians and reptiles, of which 24 are new distribution records for the SCNP region. Consequently, our findings increase the known herpetofauna in the region to 94 species. Despite their proximity, the two sites in the SCNP region exhibited only 42% similarity in species composition, and they differed significantly from other areas within the Caatinga. Furthermore, even the closer Caatinga areas presented differences in species composition, highlighting the necessity to evaluate biodiversity across the landscape and contribute to understanding biogeographic patterns.

Resumo É repetidamente enfatizada a necessidade de caracterizar a biodiversidade vivente em ecossistemas tropicais. No entanto, os estudos de inventário ainda estão progredindo lentamente em ecossistemas secos, levando à subestimação de sua verdadeira biodiversidade e dificultando os esforços de conservação. Neste estudo, apresentamos dados primários e secundários, juntamente com uma lista atualizada de anfíbios e répteis de duas localidades na região de São Francisco-Gurguéia, do Piauí. Além disso, comparamos a composição de espécies entre nove áreas dentro da Caatinga, que foram amostradas usando métodos padronizados nos últimos dez anos, para examinar padrões espaciais mais amplos de composição da comunidade. Para estudar répteis e anfíbios, utilizamos métodos e esforços de amostragem semelhantes em duas áreas na região do Parque Nacional da Serra das Confusões (PNSC). Nossos levantamentos registraram um total de 73 espécies de anfíbios e répteis, das quais 24 são novos registros de distribuição para a região do PNSC. Consequentemente, nossos resultados aumentam a herpetofauna conhecida na região para 94 espécies. Apesar da proximidade, os dois locais na região do PNSC exibiram apenas 42% de similaridade na composição de espécies e diferiram significativamente de outras áreas dentro da Caatinga. Mesmo áreas mais próximas da Caatinga apresentaram diferenças na composição de espécies, destacando a necessidade de avaliar a biodiversidade em toda a paisagem e contribuir para a compreensão de padrões biogeográficos.

https://doi.org/10.1590/1676-0611-bn-2023-1520

Analysis of Nerve Endings in the Superior Labrum-Biceps Complex by Fluorescence Immunohistochemistry and Confocal Laser Microscopy
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Cavalcante, Maria L. C.

; Fernandes, Eduardo G.

; Pinheiro Júnior, José A.

; Jamacaru, Francisco Vagnaldo F.

; Coelho, José Victor de V.

; Leite, José Alberto Dias

Revista Brasileira de Ortopedia

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Out 2022, Volume 57 Nº 5 Páginas 863 - 867

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Objetivos As estruturas capsulo-ligamentares do ombro funcionam como estabilizadores estáticos, juntamente com os músculos do bíceps e do manguito rotador, aumentando a superfície de contato da cavidade glenoide. Terminações nervosas livres e mecanorreceptores foram identificados no ombro; no entanto, existem alguns estudos que descrevem a presença desses nervos na inserção do bíceps. Este estudo teve como objetivo descrever a morfologia e distribuição de terminações nervosas utilizando imunofluorescência com protein gene product 9.5 (PGP 9.5) e microscopia confocal. Métodos Foram estudados seis complexos labrum-bíceps de seis cadáveres congelados frescos. Os espécimes foram cortados coronalmente e preparados pelo método de imunofluorescência. Tanto em hematoxilina e eosina (H&E) quanto em imunofluorescência, foi descrita a organização do tecido conjuntivo com fibras paralelas de colágeno. Resultados No estudo de H&E, foram visualizadas estruturas vasculares e algumas estruturas nervosas, que foram identificadas pela presença alongada da célula nervosa. Todas as amostras analisadas com imunofluorescência e microscopia confocal demonstraram baixa ocorrência de morfotipos de corpúsculos sensoriais e terminações nervosas livres. Identificamos terminações nervosas livres localizadas no labrum, inserção bicipital e terminações nervosas esparsas ao longo do tendão. Terminais corpusculares com aspecto fusiforme, cuneiforme e oval foram identificados no tendão. Conclusão Esses achados corroboram a hipótese de que a geração de dor nas lesões labrais superiores de anterior a posterior (SLAP, na sigla em inglês) deriva da parte mais proximal do cabo longo do bíceps e ainda mais do labrum superior. Estudos quantitativos futuros com um número maior de espécimes podem fornecer mais informações sobre esses sistemas sensoriais.

Abstract Objectives The capsuloligamentous structures of the shoulder work as static stabilizers, together with the biceps and rotator cuff muscles, increasing the contact surface of the glenoid cavity. Free nerve endings and mechanoreceptors have been identified in the shoulder; however, there are a few studies that describe the presence of these nerves in the biceps' insertion. The present study aimed to describe the morphology and distribution of nerve endings using immunofluorescence with protein gene product 9.5 (PGP 9.5) and confocal microscopy. Methods Six labrum-biceps complexes from six fresh-frozen cadavers were studied. The specimens were coronally cut and prepared using the immunofluorescence technique. In both hematoxylin and eosin (H&E) and immunofluorescence, the organization of the connective tissue with parallel collagen fibers was described. Results In the H&E study, vascular structures and some nerve structures were visualized, which were identified by the elongated presence of the nerve cell. All specimens analyzed with immunofluorescence and confocal microscopy demonstrated poor occurrence of morphotypes of sensory corpuscles and free nerve endings. We identified free nerve endings located in the labrum and in the bicipital insertion, and sparse nerve endings along the tendon. Corpuscular endings with fusiform, cuneiform, and oval aspect were identified in the tendon. Conclusion These findings support the hypothesis that the generation of pain in the superior labral tear from Anterior to posterior (SLAP) lesions derives from the more proximal part of the long biceps cord and even more from the upper labrum. Future quantitative studies with a larger number of specimens may provide more information on these sensory systems.

https://doi.org/10.1055/s-0040-1722579

Benzophenone Derivatives Showed Dual Anti-Inflammatory and Antiproliferative Activities by Inhibiting COX Enzymes and Promote Cyclin E Downregulation
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Folquitto, Laís R. S.

; Souza, Thiago B. de

; Januario, Jaqueline P.

; Nascimento, Isadora M.

; Brandão, Brenda T. V.

; Moreira, Maria E. C.

; Horvath, Renato O.

; Santos, Marcelo H.

; Coelho, Luiz F. L.

; Veloso, Marcia P.

; Soares, Marisi G.

; Carvalho, Diogo T.

; Ionta, Marisa

; Chagas-Paula, Daniela A.

; Dias, Danielle F.

Journal of the Brazilian Chemical Society

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Abr 2022, Volume 33 Nº 4 Páginas 348 - 360

Resumo: En | Texto: En | PDF: En

Considering the promising antitumor effects of compounds with dual anti-inflammatory and antiproliferative activities, thus benzophenones analogs (2-7) were evaluated on in vivo anti-inflammatory assay and molecular docking analysis. Those with the best molecular docking results were in vitro evaluated on cyclooxygenase (COX) enzymes and tested regarding antiproliferative activity. All derivatives displayed in vivo anti-inflammatory activity. Among them, the substances 2’-hydroxy-4’-benzoylphenyl-β-D-glucopyranoside (4), 4-hydroxy-4’-methoxybenzophenone (5) and 4’-(4’’-methoxybenzoyl)phenyl-β-D-glucopyranoside (7) showed the best values of Glide Score in COX-2 docking evaluation and 4 and 5 selectively inhibited COX-2 and COX-1 in vitro enzymatic assay, respectively. Thus, 4 and 5 were tested against breast cancer (MCF-7, MDA MB-231, Hs578T) and non-small-cell-lung cancer (A549) cell lines. The estrogen-positive MCF-7 cell line was more responsive compared to other tested cell lines. They induced cell cycle arrest at G1/S transition in MCF-7 cell line once there was an increase in G0/G1 population with concomitant reduction of S population. The antiproliferative activity of these substances on MCF-7 was associated with their ability to inhibit cyclin E expression, a critical regulator of G1/S transition. Taken together, the data indicate that 4 and 5 have dual anti-inflammatory and antiproliferative activities and support further studies to evaluate their antitumor potential.

https://doi.org/10.21577/0103-5053.20210154

Size-dependent sex allocation in Solanum lycocarpum St. Hil. (Solanaceae)
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Coelho, F. F.

; Damasceno, A. G.

; Fávaro, A.

; Teodoro, G. S.

; Langsdorff, L. P.

Brazilian Journal of Biology

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2022, Volume 82 elocation e237575

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo A alocação de recursos para reprodução pode mudar dependendo do tamanho, conforme previsto pela alocação sexual dependente do tamanho. Essa teoria é baseada no fato de que indivíduos pequenos investirão na alocação sexual com menor custo de produção, geralmente do sexo masculino. Nas plantas, existem algumas espécies andromonoicas, presença de hermafrodita e flores masculinas no mesmo indivíduo. A andromonoicia fornece uma estratégia para alocar recursos de maneira ideal às funções masculina e feminina, desenvolvendo uma estratégia reprodutiva de economia de energia. Assim, nosso objetivo foi investigar a alocação sexual dependente do tamanho em Solanum lycocarpum St. Hil. Testamos a hipótese de que plantas de maior tamanho investirão na produção de flores hermafroditas, pois indivíduos mais altos economizam mais disponibilidade de recursos para investir em estruturas mais complexas que envolvem maior gasto de energia. A espécie estudada foi S. lycocarpum, uma espécie andromonoica. De junho de 2016 a março de 2017, os dados foram coletados em 38 indivíduos, divididos em dois grupos: o maior grupo de plantas (n = 18; altura = 3-5 m) e o menor grupo de plantas (n = 20; altura = 1-2 m). Nossos dados mostram que houve efeito do tamanho da planta na produção de flores e na alocação sexual. As plantas maiores apresentaram mais flores e maior produção de flores hermafroditas. Além disso, observamos uma relação alométrica entre as características da flor, com investimentos proporcionais em biomassa, tamanho da antera e tamanho do gineceu. Nossos resultados estão de acordo com a teoria de alocação de sexo dependente de tamanho e a hipótese de andromonoicia relacionada a mecanismos para a alocação ótima de recursos para a função masculina e feminina.

Abstract Resource allocation to reproduction can change depending on size, as predicted by the size-dependent sex allocation. This theory is based on the fact that small individuals will invest in the allocation of sex with lower cost of production, usually male gender. In plants, there are some andromonoecy species, presence of hermaphrodite and male flowers in the same individual. Andromonoecy provides a strategy to optimally allocate resources to male and female function, evolving a reproductive energy-saving strategy. Thus, our objective was to investigate the size-dependent sex allocation in Solanum lycocarpum St. Hil. We tested the hypothesis that plants with larger size will invest in the production of hermaphrodite flowers, because higher individuals have greater availability of resources to invest in more complex structures involving greater energy expenditure. The studied species was S. lycocarpum, an andromonoecious species. From June 2016 to March 2017 the data were collected in 38 individuals, divided in two groups: the larger plant group (n=18; height=3-5 m) and the smaller plant group (n=20; height=1-2 m).Our data show that there was effect of plant size on the flower production and the sexual gender allocation. The larger plants showed more flowers and higher production of hermaphrodite flowers. Furthermore, in the flower scale, we observed allometric relationship among the flower’s traits with proportional investments in biomass, anther size and gynoecium size. Our results are in agreement with size-dependent sex allocation theory and andromonoecy hypothesis related to mechanisms for optimal resource allocation to male and female function.

https://doi.org/10.1590/1519-6984.237575 246 downloads

10.

The methodology of the electrical conductivity test for Carthamus tinctorius L. seeds
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Silva, Ingrid M. H. de L. e

; Silva, Givanildo Z. da

; Coelho, Mirelle V.

; Gama, Gabriela F.

; Silva, Amalia A. S.

; Machado, Carla G.

Revista Brasileira de Engenharia Agrícola e Ambiental

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Ago 2021, Volume 25 Nº 8 Páginas 553 - 559

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO O teste de condutividade elétrica destaca-se por gerar respostas rápidas para diferenciação de lotes, no entanto, necessita-se de estudos visando o aprimoramento desta prática em sementes de cártamo. Objetivou-se verificar a eficiência e estabelecer metodologia do teste de condutividade elétrica para sementes de cártamo. Inicialmente, 12 lotes foram caracterizados quanto às propriedades físicas e potencial fisiológico. Para condutividade elétrica, o experimento foi efetuado em três etapas com os 12 lotes: primeiro determinou-se o período de embebição (2, 4, 6, 8, e 16 horas), em seguida avaliou-se o volume (50, 75, 100, 150 e 200 mL) e por fim determinou-se a quantidade de sementes (25 e 50). O teste de condutividade elétrica utilizando 25 sementes embebidas em 50 mL de água destilada no período de seis horas possibilita a diferenciação de lotes de sementes de cártamo. A análise multivariada dos componentes principais é eficiente na discriminação do vigor de lotes de sementes de cártamo.

ABSTRACT The electrical conductivity test stands out for generating quick responses for lot differentiation; however, studies are needed to improve this practice in safflower seeds. This study aimed to verify the efficiency and establish a methodology of the electrical conductivity test for safflower seeds. Initially, 12 lots were characterized regarding physical properties and physiological potential. For electrical conductivity, the experiment was carried out in three stages with the 12 lots: firstly, the soaking period was determined (2, 4, 6, 8, and 16 hours), then the volume was evaluated (50, 75, 100, 150, and 200 mL), and finally the number of seeds (25 and 50 seeds) was determined. The electrical conductivity test using 25 seeds in 50 mL of distilled water for six hours of soaking enables the differentiation of safflower seed lots. Multivariate analysis of principal components is efficient in discriminating the vigor of safflower seed lots.

https://doi.org/10.1590/1807-1929/agriambi.v25n8p553-559 395 downloads

11.

Morita-Baylis-Hillman Reaction with 7-Chloroquinoline Derivatives-New Compounds with Potential Anticancer Activity
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Oliveira, João Paulo G.

; Caleffi, Guilherme S.

; Silva, Everton P.

; Coelho, Maísa C.

; Castro, Aleff C.

; Mendes, Rhuan K. S.

; Olegário, Tayná R.

; Lima-Junior, Claudio G.

; Vasconcellos, Mario L. A. A.

; Souza, Júlia L. C.

; Souza, Sílvia M.

; Militão, Gardênia C. G.

; Vaz, Boniek G.

; Ramalho, Ruver R. F.

Journal of the Brazilian Chemical Society

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Fev 2021, Volume 32 Nº 2 Páginas 347 - 354

Resumo: En | Texto: En | PDF: En

Morita-Baylis-Hillman adducts (MBHA) is a class of polyfunctional molecules that has been standing out due to their versatility and expressive biological activities. Therefore, this paper describes the synthesis and antiproliferative activity of some new MBHA/7-choroquinoline hybrids. The Michael acceptors were obtained starting from 4,7-dichloroquinoline which were submitted to the Morita-Baylis-Hillman reaction with ortho, meta and para-nitrobenzaldehyde. The in vitro screening of the synthetized MBHA against NCI-H292, HCT-116 and MCF-7 cancer cells suggests the influence of the spacer chain in its inhibition potential. The 50% inhibitory concentration (IC50) obtained in the antiproliferative assay using MCF-7, HCT-116, HL-60 and NCI-H292 cancer cells indicate expressive cytotoxic potential of the adducts containing nitro group in the ortho position, with IC50 of 4.60 µmol L-1. MBHA/7-choroquinoline hybrids were more active than MBHA described in literature, indicating the improvement of the cytotoxic effect due to 7-chloroquinoline moiety in the molecular structure, with maximum selectivity index values of 11.89.

https://doi.org/10.21577/0103-5053.20200185 303 downloads

12.

Evaluation of hematology, general serum biochemistry, bone turnover markers and bone marrow cytology in a glucocorticoid treated ovariectomized sheep model for osteoporosis research
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COELHO, CATARINA A.

; BORDELO, JOÃO P.

; CAMASSA, JOSÉ A.

; BARROS, VERA A.

; BABO, PEDRO S.

; GOMES, MANUELA E.

; REIS, RUI L.

; AZEVEDO, JORGE T. DE

; REQUICHA, JOÃO F.

; FAÍSCA, PEDRO

; CARVALHO, PEDRO P.

; VIEGAS, CARLOS A.

; DIAS, ISABEL R.

Anais da Academia Brasileira de Ciências

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2020, Volume 92 Nº 4 elocation e20200435

Resumo: En | Texto: En | PDF: En

Abstract Osteoporosis is a metabolic disorder characterized by a loss of bone mass and structure and increasing the risk of fragility fractures, mostly among postmenopausal women. Sheep is a recognized large animal model for osteoporosis research. An experimental group of ewes (3-4 years old) was subjected to ovariectomy (OVX) and weekly glucocorticoid (GC) application for 24 weeks and compared with a sham control group. Blood and bone marrow parameters were analyzed before and 24 weeks after OVX and GC administration. Osteopenia was confirmed through micro-computed tomography and histomorphometric analysis of L4 vertebra in the study end. A statistically significant increase was observed in mean corpuscular volume, mean cell hemoglobin and monocytes and a decrease in red blood count and eosinophils (p<0.05). Alkaline phosphatase (ALP), gamma-glutamyl transpeptidase, magnesium and α1-globulin increased, and creatinine, albumin, sodium and estradiol decreased (p<0.05). A slight decrease of bone formation markers (bone ALP and osteocalcin) and an increase of bone resorption markers (C-terminal telopeptides of collagen type 1 and tartrate-resistant acid phosphatase) were observed, but without statistical significance. This study aims to contribute to better knowledge of sheep as a model for osteoporosis research and the consequences that a performed induction protocol may impose on organic metabolism.

https://doi.org/10.1590/0001-3765202020200435 677 downloads

13.

Distribution uniformity in drip units applying dilutions of treated water produced by oil exploration
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Vale, Hudson S. M.

; Costa, Danniely O.

; Batista, Rafael O.

; Oliveira, Francisco de A. de

; Medeiros, José F. de

; Coelho, Daniela da C. L.

Revista Brasileira de Engenharia Agrícola e Ambiental

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2020, Volume 24 Nº 6 Páginas 394 - 401

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Objetivou-se, com este estudo, avaliar a uniformidade de distribuição em unidades gotejadoras aplicando diluições de água tratada produzida pela exploração do petróleo. O experimento foi montado no delineamento inteiramente casualizado em esquema de parcelas sub-subdivididas, com três repetições; tendo nas parcelas os tratamentos P0 - 100% de água subterrânea, controle; P10 - 90% de água subterrânea e 10% de água produzida; P20 - 80% de água subterrânea e 20% de água produzida; P30 - 70% de água subterrânea e 30% de água produzida; e P40 - 60% de água subterrânea e 40% de água produzida; nas subparcelas os tipos de gotejadores não autocompensantes (D1 - 1,6 L h-1, Netafim Super Typhoon, D2 - 1,6 L h-1, Netafim Streamline e D3 - 1,7 L h-1, NaanDanJain TalDrip) e nas subsubparcelas os tempos de avaliação (0, 40, 80, 120 e 160 h). Foram avaliados os coeficientes de uniformidade de distribuição e de uniformidade estatístico a cada 40 h, até completar 160 h. O pH das diluições foi o atributo que representou maior risco de obstrução aos emissores. Ainda em relação às diluições, P40 ocasionou maior desuniformidade de aplicação em D2, enquanto, os maiores valores de uniformidade de aplicação foram alcançados nos tratamentos P10 e P20 em D1 e D3, indicando eficácia da diluição na minimização da obstrução.

ABSTRACT The objective of this study was to evaluate the uniformity of distribution in drip units applying dilutions of treated water produced by oil exploration. The experiment was set up in a completely randomized design in a split-split-plot scheme, with three replicates. The plots were composed of the treatments P0 - 100% groundwater, control; P10 - 90% groundwater and 10% produced water; P20 - 80% groundwater and 20% produced water; P30 - 70% groundwater and 30% produced water; and P40 - 60% groundwater and 40% produced water; the subplots had the types of non-pressure compensating drippers (D1 - 1.6 L h-1, Netafim Super Typhoon, D2 - 1.6 L h-1, Netafim Streamline and D3 - 1.7 L h-1, NaanDanJain TalDrip); and the sub-subplots contained the evaluation times (0, 40, 80, 120 and 160 h). Distribution uniformity and statistical uniformity coefficients were evaluated every 40 h, until completing 160 h. The pH of the dilutions was the attribute posing the highest risk of clogging to the emitters. Also in relation to dilutions, P40 caused greater application non-uniformity in D2, while the highest values of application uniformity were achieved in treatments P10 and P20 with D1 and D3, indicating the effectiveness of dilution in minimizing clogging.

https://doi.org/10.1590/1807-1929/agriambi.v24n6p394-401 514 downloads

14.

Synthesis and Biological Evaluation of New Eugenol-Derived 1,2,3- Triazoles as Antimyco bacterial Agents
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Santos, Thiago dos

; Coelho, Camila M.

; Elias, Thiago C.

; Siqueira, Fallon S.

; Nora, Eloísa S. S. D.

; Campos, Marli M. A. de

; Souza, Gabriel A. P. de

; Coelho, Luiz F. L.

; Carvalho, Diogo T.

Journal of the Brazilian Chemical Society

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Jul 2019, Volume 30 Nº 7 Páginas 1425 - 1436

Resumo: En | Texto: En | PDF: En

Eugenol has diverse biological properties including antimycobacterial activity, and the triazole ring is an important heterocycle in antimycobacterial compounds. Therefore, this research aimed to synthesize novel eugenol-derived 1,2,3-triazole as antimycobacterial agents with interesting cytotoxic profile and pharmacological assets. Sixteen compounds were obtained and characterized by nuclear magnetic resonance (NMR), infrared (IR), and high-resolution mass spectrometry (HRMS). Among them, the best growth inhibition properties from a microdilution assay were observed for three derivatives: a benzylic ether (minimum inhibitory concentration (MIC) = 48.89 µM) against Mycobacterium abscessus (ATCC 19977), an O-galactosyde (MIC = 31.76 µM) against Mycobacterium massiliense (ATCC 48898) and a sulfonate (MIC = 88.64 µM) against Mycobacterium fortuitum (ATCC 6841). They can form biofilms, and the infection progression is challenging to control due to multi-drug resistance profiles against diverse antibiotics. In conclusion, the above-mentioned compounds represent starting points in the search of bioactive molecules against mycobacteria with low cytotoxicity and better pharmacological profiles.

https://doi.org/10.21577/0103-5053.20190038 484 downloads

15.

CHARACTERIZATION-PERFORMANCE OF ZnO AND ZnO/ZnFe2O4 CATALYST USING ARTIFICIAL AND SOLAR LIGHT FOR MERCURY (II) REDUCTION
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Coelho, Ana L. S.

; Almeida Neto, Ambrósio F. de

; Ivashita, Flávio F.

; Lenzi, Giane G.

; Jorge, Luiz M. de M.

; Santos, Onélia A. A. dos

Brazilian Journal of Chemical Engineering

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Jun 2019, Volume 36 Nº 2 Páginas 797 - 810

Resumo: En | Texto: En | PDF: En

Abstract This study describes the application of ZnO commercial oxide and ZnO/ZnFe2O4 mixed oxides to reduce the mercury ion (Hg2+) in the aqueous phase. The catalysts synthesized were characterized by thermogravimetric analysis, adsorption and desorption of N2, scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, photoacoustic spectroscopy and Mössbauer spectroscopy. The heterogeneous photocatalysis process was studied in synthetic effluent treatment containing mercury chloride (HgCl2) in a batch and solar reactor. The results indicated that commercial ZnO catalyst totally reduced the Hg (II), in all conditions and reaction systems studied. On the other hand, for the mixed oxides (ZnO/ZnFe2O4), there was a decrease in the reduction efficiency in the batch reactor, indicating no ability to reduce Hg(II) in the presence of sunlight.

https://doi.org/10.1590/0104-6632.20190362s20180280 1062 downloads

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