ABSTRACT Objective To assess the level of exposure to pesticides and its correlation with perceived health indicators and injury biomarkers (genotoxic alterations and those caused by butyrylcholinesterase enzyme activity) in the population of pesticide applicators in extensive crops (PAEC) in Córdoba, Argentina. Methods Transversal study, in PAEC (n = 47) randomly selected from a sample of 2000, and non-exposed subject controls (n = 52). The sociodemographic variables, exposure conditioning, and perceived health were surveyed by means of a self-administered questionnaire; biological indicators of genotoxicity: micronuclei, chromosomal aberrations and kite assay, and butyrylcholinesterase activity. Results 40% of PAEC have over 10 years’ length of service and almost 50% of them reside less than 500 m from the sprinkled fields; they report low rates of personal protective equipment use while mixing, applying, or repairing the equipment. General, cardio-respiratory, and dermatological symptoms were greater among PAEC (p <0.05) as well as indicators of genotoxic injury (p <0.001). The butyrylcholinesterase activity was negatively associated with levels of exposure to pesticides. Conclusions The PAEC show an important negative impact on health linked to exposure to pesticides. The exposure scales associated to the use of biomarkers were a useful tool for monitoring pesticide applicators’ health.

RESUMEN Objetivo Evaluar el nivel de exposición a plaguicidas y su correlación con indicadores de salud percibida y biomarcadores de daño (alteraciones genotóxicas y de actividad enzimática de la butirilcolinesterasa), en la población de agroaplicadores de cultivos extensivos (ACE) de la Provincia de Córdoba, Argentina. Método Estudio transversal, en ACE (n = 47) seleccionados aleatoriamente de una muestra de 2000, y sujetos no expuestos controles (n = 52). Se relevaron variables sociodemográficas, condicionantes de exposición y de salud percibida, mediante cuestionario autoadministrado; indicadores biológicos de genotoxicidad: micronúcleos, aberraciones cromosómicas y ensayo cometa, y actividad de butirilcolinesterasa. Resultados El 40% de los ACE tiene una antigüedad mayor de 10 años y casi el 50% reside a menos de 500 m de campos asperjados; reportan bajas tasas de uso de equipo de protección personal durante la mezcla, aplicación o reparación de equipos. Los síntomas generales, cardiorrespiratorios y dermatológicos fueron mayores entre los ACE (p <0,05), así como los indicadores de daño genotóxico (p <0,001). La actividad butirilcolinesterasa se asoció negativamente a niveles de exposición a plaguicidas. Conclusiones Los ACE presentan un importante impacto negativo en la salud vinculado a la exposición a plaguicidas. Las escalas de exposición asociadas al uso de biomarcadores resultaron una herramienta útil para la vigilancia de la salud de los agroaplicadores.

ABSTRACT Objective To assess the level of exposure to pesticides and its correlation with perceived health indicators and injury biomarkers (genotoxic alterations and those caused by butyrylcholinesterase enzyme activity) in the population of pesticide applicators in extensive crops (PAEC) in Córdoba, Argentina. Methods Transversal study, in PAEC (n = 47) randomly selected from a sample of 2000, and non-exposed subject controls (n = 52). The sociodemographic variables, exposure conditioning, and perceived health were surveyed by means of a self-administered questionnaire; biological indicators of genotoxicity: micronuclei, chromosomal aberrations and kite assay, and butyrylcholinesterase activity. Results 40% of PAEC have over 10 years’ length of service and almost 50% of them reside less than 500 m from the sprinkled fields; they report low rates of personal protective equipment use while mixing, applying, or repairing the equipment. General, cardio-respiratory, and dermatological symptoms were greater among PAEC (p <0.05) as well as indicators of genotoxic injury (p <0.001). The butyrylcholinesterase activity was negatively associated with levels of exposure to pesticides. Conclusions The PAEC show an important negative impact on health linked to exposure to pesticides. The exposure scales associated to the use of biomarkers were a useful tool for monitoring pesticide applicators’ health.

RESUMEN Objetivo Evaluar el nivel de exposición a plaguicidas y su correlación con indicadores de salud percibida y biomarcadores de daño (alteraciones genotóxicas y de actividad enzimática de la butirilcolinesterasa), en la población de agroaplicadores de cultivos extensivos (ACE) de la Provincia de Córdoba, Argentina. Método Estudio transversal, en ACE (n = 47) seleccionados aleatoriamente de una muestra de 2000, y sujetos no expuestos controles (n = 52). Se relevaron variables sociodemográficas, condicionantes de exposición y de salud percibida, mediante cuestionario autoadministrado; indicadores biológicos de genotoxicidad: micronúcleos, aberraciones cromosómicas y ensayo cometa, y actividad de butirilcolinesterasa. Resultados El 40% de los ACE tiene una antigüedad mayor de 10 años y casi el 50% reside a menos de 500 m de campos asperjados; reportan bajas tasas de uso de equipo de protección personal durante la mezcla, aplicación o reparación de equipos. Los síntomas generales, cardiorrespiratorios y dermatológicos fueron mayores entre los ACE (p <0,05), así como los indicadores de daño genotóxico (p <0,001). La actividad butirilcolinesterasa se asoció negativamente a niveles de exposición a plaguicidas. Conclusiones Los ACE presentan un importante impacto negativo en la salud vinculado a la exposición a plaguicidas. Las escalas de exposición asociadas al uso de biomarcadores resultaron una herramienta útil para la vigilancia de la salud de los agroaplicadores.

Peri-urban infestations with triatomine bugs, their sources and their dynamics have rarely been investigated. Here, we corroborated the reported occurrence of Triatoma infestans in a peri-urban area and in neighbouring rural houses in Pampa del Indio, in the Argentine Chaco, and identified its putative sources using spatial analysis and demographic questionnaires. Peri-urban householders reported that 10% of their premises had triatomines, whereas T. infestans was collected by timed manual searches or community-based surveillance in only nine (3%) houses. Trypanosoma cruzi-infected T. infestans and Triatoma sordida were collected indoors only in peri-urban houses and were infected with TcV and TcI, respectively. The triatomines fed on chickens, cats and humans. Peri-urban infestations were most frequent in a squatter settlement and particularly within the recently built mud houses of rural immigrants, with large-sized households, more dogs and cats and more crowding. Several of the observed infestations were most likely associated with passive bug transport from other sources and with active bug dispersal from neighbouring foci. Thus, the households in the squatter settlement were at a greater risk of bug invasion and colonisation. In sum, the incipient process of domestic colonisation and transmission, along with persistent rural-to-urban migratory flows and unplanned urbanisation, indicate the need for active vector surveillance and control actions at the peri-urban interface of the Gran Chaco.

El objetivo del estudio fue evaluar la capacidad de la vegetación para mitigar el ruido en el valle de Aburrá. La metodología comprendió dos fases: la primera consistió en la revisión de información secundaria relacionada con dicha función de la vegetación, lo que permitió obtener las bases conceptuales para el posterior desarrollo experimental. En la segunda fase se realizó la medición de los niveles de ruido a 10 m de la fuente sonora (vía de alto tráfico vehicular) en zonas verdes públicas urbanas con diferentes coberturas vegetales (arbórea, arbustiva y arbórea-arbustiva) y sin vegetación. Los resultados no arrojaron diferencias significativas entres los sitios con vegetación y sin ella, lo que permite inferir que en las condiciones del estudio, caracterizadas por contar con unos pocos individuos arbóreos o arbustivos dispersos y poco densos, la vegetación no cumple un papel significativo en la disminución del ruido. Por lo tanto, para su control en zonas urbanas deben tomarse medidas diferentes a la plantación de árboles, o establecer barreras vivas más anchas, largas, altas y densas.

The objective of the study was to assess the capacity of vegetation to reduce the noise in the Aburrá valley. The methodology comprised two phases: the first was the revision of secondary information related to the role of vegetation and to obtain the conceptual bases for further experimental development. The second phase was the measurement of noise levels 10 m from the sound source (high traffic road) in urban public green zones with different plant coverage (tree, shrub, and a mixture of trees and shrubs) and without vegetation. Results showed no significant differences between the sites with and without vegetation, allowing us to infer that under conditions of the study, characterized by having few individuals of trees and shrubs dispersed, the vegetation does not play a significant role in reducing noise. Therefore, for its control in urban areas measures other than the planting of trees must be taken, or wider, longer, denser, and higher live barriers should be established.

O objetivo do estudo foi avaliar a capacidade da vegetação para mitigar o ruído no vale de Aburrá. A metodologia compreendeu duas fases: a primeira consistiu na revisão de informação secundária relacionada com dita função da vegetação, o que permitiu obter as bases conceptuais para o posterior desenvolvimento experimental. Na segunda fase se realizou a medição dos níveis de ruído a 10 m da fonte sonora (via de alto tráfico veicular) em zonas verdes públicas urbanas com diferentes coberturas vegetais (arbórea, arbustiva e arbórea-arbustiva) e sem vegetação. Os resultados não arrojaram diferenças significativas entre os lugares com vegetação e sem ela, o que permite inferir que sob as condições do estudo, caracterizadas por contar com uns poucos indivíduos arbóreos ou arbustivos dispersos e pouco densos, a vegetação não cumpre um papel significativo na diminuição do ruído. Portanto, para seu controle em zonas urbanas devem tomar-se medidas diferentes à plantação de árvores, ou estabelecer barreiras vivas mais largas, compridas, altas e densas.