Introduction It is well known that Parkinson’s disease is related to a deficit of dopamine (DA) in the region of the brain called the corpus striatum. The aim of this work is to demonstrate the possibility of in vitro closed-loop control of the DA concentration levels. Methods By applying the electrochemical technique of fast scan cyclic voltammetry (FSCV), the measured values were compared with previously selected ones, and the system made decisions to control infusion pumps by dynamically adjusting the DA concentration in a continuous flow injection cell. Low-cost hardware was used for the acquisition and control signals (Arduino board), whereas for processing the collected data, graphical programming software (LabView) was used. Results The resolution of the system was approximately 0.4 µmol/L, with a time correction of the concentration adjustable between 1 and 90 seconds. The system allowed control of the DA concentration between 1 and 10 µmol/L with an error of approximately +/– 0.8 µmol/L. Conclusion Although designed to control the DA concentration, the system could be used to control, within the range of the developed FSCV, the concentration of other substances or to turn on brain stimulators. These results encourage the possibility of using the system in clinical studies (in vivo).