Resumo Kiwis minimamente processados necessitam de técnicas para manter a qualidade dos frutos durante a conservação e o período de prateleira. Aditivos combinados à radiação ultravioleta podem ser uma alternativa para assegurar a qualidade das frutas por mais tempo de conservação. Neste contexto, foi proposto avaliar a eficiência do uso da radiação UV-C e diferentes aditivos na prevenção do escurecimento e conservação de kiwis ‘Bruno’ minimamente processados. As fatias de kiwi foram submetidas a soluções contendo os seguintes tratamentos: controle, ácido ascórbico a 1%; isoascorbato de sódio a 1%; ácido cítrico a 1%, depois, utilizou-se por dois minutos a radiação UV-C na intensidade de 2,71 kJ m-2. As bandejas foram armazenadas em câmara fria a 4 ± 1 ºC de temperatura, com umidade relativa de 85% a 90%, e armazenadas por períodos de 3, 6 e 9 dias. Avaliaram-se perda de massa, coloração da polpa, sólidos solúveis, acidez titulável, incidência de podridão, compostos fenólicos totais e atividade antioxidante. O uso de aditivos melhora a conservação do kiwi minimamente processado, com destaque para o isoascorbato de sódio e o ácido cítrico. Os frutos de kiwi da cultivar ‘Bruno’ podem ser armazenados por até 6 dias sob refrigeração com o uso de aditivos. Após esse período, a perda de massa é elevada. O uso de UV-C não alterou a qualidade dos kiwis durante o armazenamento, podendo ter seu uso dispensado.

Abstract Minimally processed kiwis need techniques to maintain the quality of the fruits during conservation and shelf life. The additives combined with ultraviolet radiation can be an alternative to ensure the quality of the fruits for a longer conservation period. In this context, it was proposed to evaluate the efficiency of the use of UV-C radiation and different additives in preventing the browning and conservation of minimally processed 'Bruno' kiwis. The kiwi slices were subjected to solutions containing the following treatments: control, ascorbic acid 1%; 1% sodium isoascorbate; citric acid 1%, after two minutes UV-C radiation was used at an intensity of 2.71 kJ m-2. The trays were stored in a cold chamber at 4 ± 1º C of temperature, with a relative humidity of 85% to 90%, and stored for periods of 3, 6 and 9 days. It was evaluated: mass loss, pulp color, soluble solids, titratable acidity, the incidence of rot, total phenolic compounds, and antioxidant activity. The use of additives improves the conservation of minimally processed kiwi, with emphasis on sodium isoascorbate and citric acid. The kiwi fruits of the cultivar 'Bruno' can be stored for up to 6 days under refrigeration with the use of additives, after which the loss of mass is high. The use of UV-C did not change the quality of kiwis during storage, and their use may be dispensed with.

ABSTRACT: The aim of this in vitro study was to investigate the influence of ethylenediaminetetraacetic acid (EDTA) associated with the benzalkonium chloride (BAK) on the adhesion and formation of Enterococcus faecalis biofilms attached to coated dentin. Discs standard bovine dentin blocks were treated with the coating materials evaluated: Saline solution (control), 17 % EDTA, 17 % EDTA associated with 1 % BAK for 5 minutes and subsequently washed with saline solution. Afterwards, biofilms of E. faecalis (ATCC 29212) were grown on the surface of coated dentin blocks for time intervals of 1 hour and 7 days (n = 20) and were subsequently washed with phosphate-buffered saline (PBS). Bacterial viability and total biovolume were analyzed by confocal laser scanning microscopy (CLSM) using the Live/Dead technique. Nonparametric Kruskal-Wallis followed by Dunn tests were used to determine statistical differences (a = 5 %). The 17 % EDTA + 1 % BAK group showed significantly lower biovolume and bacterial viability values at the end of 1 hour (p < 0.05). After 7 days of contamination, the 17 % EDTA and 17 % EDTA + 1 % BAK groups showed similar results that differed statistically from those of the control group (p < 0.05). The saline solution group showed higher values. The use of BAK associated with EDTA on dentin blocks surfaces before exposure to contamination was able to interfere in the adhesion of E. faecalis to dentin. Also, dentin treatment by BAK associated with a chelating agent influences the secondary biofilm formation, which could have important effects on the long-term success of root canal treatment.

RESUMEN: El objetivo del estudio consistió en investigar in vitro, la influencia del ácido etilendiamino-tetraacético (EDTA) con cloruro de benzalconio (BAK) en la adhesión y formación de biopelículas de Enterococcus faecalis a la dentina. Discos de dentina bovina fueron tratadas con solución salina (control), 17 % de EDTA, 17% de EDTA asociado con 1 % de BAK durante 5 minutos y lavadas con solución salina. Las biopelículas de E. faecalis (ATCC 29212) se cultivaron sobre los discos de dentina durante intervalos de tiempo de 1 hora y 7 días (n = 20), lavados con solución salina tamponada con fosfato (PBS). La viabilidad bacteriana y el biovolumen total se analizaron mediante microscopía de barrido por láser (CLSM) utilizando la técnica Live / Dead. Se realizó prueba no paramétrica de Kruskal-Wallis, seguida por Dunn con una diferencia estadística (a = 5 %). El grupo de 17 % EDTA + 1 % BAK mostró valores significativamente menores de biovolumen y viabilidad bacteriana al final de 1 hora (p < 0,05). Después de 7 días de contaminación, los grupos de 17 % EDTA y 17 % EDTA + 1 % BAK mostraron resultados similares que diferían estadísticamente del grupo control (p < 0,05). La solución salina mostró valores más altos. La asociación de BAK con EDTA antes de la contaminación interfirió en la adhesión de E. faecalis. Además, el tratamiento de la dentina por BAK asociado con EDTA influye en la formación de biopelículas secundarias, lo que podría tener efectos importantes sobre el éxito a largo plazo del tratamiento del conducto radicular.

Abstract The aim of this study was to assess Brazilian endodontists’ level of knowledge about the coronavirus disease (COVID-19) and examine its professional repercussions. The link to the online survey that was created for this study was shared with Brazilian endodontists through social networking applications. The questionnaire contained questions that pertained to COVID-19 and its impact on dental practice. The collected data were analyzed using t-test, chi-square analysis, and analysis of variance, and the level of significance was set at 0.05. A total of 2,135 participants responded to the questionnaire, and all five Brazilian regions were represented in the sample. A total of 98.50% of endodontists reported that dental procedures can transmit COVID-19. Complete social distancing was practiced by 96.68% of the participants, and approximately 25% knew someone who had COVID-19. Moreover, in their daily practice, 72.13% of them implemented biosecurity measures that are ineffective in preventing COVID-19. Furthermore, 91.7% of them reportedly suspended elective dental procedures. Only 55.69% of them reported that they performed only emergency procedures in their workplaces. Those who believed that COVID-19 cannot be transmitted during dental procedures were less knowledgeable about the symptoms of COVID-19 (p = 0.0095). Endodontists who believed that personal protective equipment cannot prevent contamination were more knowledgeable about the symptoms of COVID-19 than their counterparts (p = 0.0003). The participating Brazilian endodontists demonstrated adequate knowledge about the risk of contamination during dental procedures and the main symptoms of COVID-19. Only some professionals reported providing emergency dental care during the pandemic.

Resumen Introducción. Varias revisiones sistemáticas y meta-análisis han sugerido que los programas de actividad física que combinan ejercicios de bajo impacto y de fuerza mantienen la capacidad funcional en adultos mayores. Objetivo. Analizar el efecto de un programa de entrenamiento acuático basado en movimientos explosivos y de impacto en los parámetros de la marcha en adultas mayores. Materiales y métodos. 60 mujeres físicamente activas (64.08±3.98 años) fueron divididas en dos grupos, uno control (CG=35) y otro de intervención (entrenamiento en piscina usando multisaltos) (IG=35). El IG entrenó por 32 semanas, 3 días a la semana, 1 hora por sesión. Se evaluó la composición corporal, la fuerza explosiva y los parámetros de la marcha sobre 6m de recorrido usando el centro de presión (COP) antes y después de participar en el programa. Resultados. Se presentaron diferencias en la fuerza explosiva y la potencia (EG vs. CG; p=0.05-001), así como cambios en los parámetros de la marcha relacionados al COP (EG vs. CG: p=0.05-001), con cambios significativos y positivos para EG. Conclusión. El programa de entrenamiento en agua con movimientos de impacto y explosivos induce ganancias en fuerza muscular y potencia, lo que mejora la capacidad de caminar. Teniendo en cuenta lo anterior, se debe considerar ofrecer una mejor disponibilidad de programas similares a esta población, ya que su participación en estos programas podría ayudarles a mejorar su capacidad funcional y, por tanto, su calidad de vida.

Abstract Introduction: Several systematic reviews and meta-analyses have suggested that physical activity programs combining low impact exercises and resistance exercises help maintaining functional capacity in older adults. Objective: To analyze the effects of an aquatic training program involving both impact and explosive exercises on gait parameters of women aged 60 and above. Materials and methods: 60 physically active women (64.08±3.98 years) were divided into 2 groups: those training in a pool by performing series ofjumps, i.e., the experimental group (EG= 35), and the control group (CG=35). EG participants trained 3 times per week during 32 weeks in an hour per session basis. Body composition measurements, explosive strength, and gait parameters (in a 6 meters long track) were assessed using the center of pressure (COP) indicator before and after participating in the training program. Results: When comparing both groups, differences in explosive strength and power (EG vs. CG; p values=from 0.05 to 001) were observed, as well as changes in gait parameters related to the COP (EG vs. CG: p = 0.05-001), in particular EG participants had significant and positive changes. Conclusion: The aquatic training program described here produced an increase in muscle strength and muscle power, thus gait parameters were improved. Bearing this in mind, an improved availability of similar programs for older adults should be considered, since their participation in these programs could help them improve their functional capacity, and, thus, their quality of life.

RESUMO Febre de origem indeterminada (FOI) é uma entidade desafiadora com presença marcante nos hospitais de todo o mundo. É definida como temperatura ≥37,8 ° C em várias ocasiões, com duração ≥3 semanas, na ausência de diagnóstico após três dias de investigação hospitalar ou três consultas ambulatoriais. As principais etiologias são de ordem infecciosa, neoplásica e doenças inflamatórias não infecciosas. O diagnóstico é baseado na história clínica e no exame físico minuciosos desses pacientes, com a finalidade de direcionar os exames complementares específicos a serem realizados em cada caso. A abordagem diagnóstica inicial do paciente com FOI deve incluir exames complementares inespecíficos. A terapia empírica não é recomendada (com poucas exceções) em pacientes com febre prolongada, uma vez que ela pode camuflar e retardar o diagnóstico e a conduta para tratar a etiologia específica. O prognóstico engloba uma mortalidade de 12-35%, variando de acordo com a etiologia de base.

SUMMARY Fever of undetermined origin (FUO) is a challenging entity with a striking presence in hospitals around the world. It is defined as temperature ≥ 37.8 ° C on several occasions, lasting ≥ three weeks, in the absence of diagnosis after three days of hospital investigation or 3 outpatient visits. The main etiologies are infectious, neoplastic, and non-infectious inflammatory diseases. The diagnosis is based on the detailed clinical history and physical examination of these patients, in order to direct the specific complementary tests to be performed in each case. The initial diagnostic approach of the FUO patient should include non-specific complementary exams. Empirical therapy is not recommended (with few exceptions) in patients with prolonged fever, as it may disguise and delay the diagnosis and conduct to treat the specific etiology. The prognosis encompasses mortality of 12-35%, varying according to the baseline etiology.

RESUMO Febre de origem indeterminada (FOI) é uma entidade desafiadora com presença marcante nos hospitais de todo o mundo, à qual uma miríade de diagnósticos diferenciais podem estar associados. É definida como temperatura axilar ≥37,8 0 C em várias ocasiões, com duração ≥ três semanas, na ausência de diagnóstico após três dias de investigação hospitalar ou três consultas ambulatoriais. As principais etiologias são de ordem infecciosa, neoplásica e reumatológica. O diagnóstico é baseado na história clínica e no exame físico minuciosos desses pacientes, com a finalidade de direcionar os exames complementares específicos a serem realizados em cada caso. A terapia empírica não é recomendada (com poucas exceções) em pacientes com febre prolongada, uma vez que ela pode camuflar e retardar o diagnóstico e a conduta para tratar a etiologia específica. O prognóstico engloba uma mortalidade de 12–35%, variando de acordo com a etiologia de base. O objetivo deste estudo é revisar os principais tópicos acerca da febre de origem indeterminada, trazendo aspectos históricos e científicos, nacionais e internacionais.

SUMMARY Fever of undetermined origin (FUO) is a challenging entity with a striking presence in hospitals around the world and can be associated with a myriad of differential diagnoses. It is defined as axillary temperature ≥ 37.8 ° C on several occasions, lasting ≥ three weeks, in the absence of diagnosis after three days of hospital investigation or three outpatient visits. The main etiologies are: infectious, neoplastic, and rheumatic. The diagnosis is based on the detailed clinical history and physical examination of these patients, in order to direct the specific complementary tests to be performed in each case. Empirical therapy is not recommended (with few exceptions) in patients with prolonged fever, as it may disguise and delay the diagnosis and conduct to treat the specific etiology. The prognosis encompasses mortality of 12% - 35%, varying according to the underlying etiology. In this sense, the objective of this study is to review the main topics about fever of undetermined origin, bringing historical and scientific aspects, national and international.

Resumo Este estudo avaliou a efetividade de um adesivo “multi-mode” (Single Bond Universal/3M) como um substituto para a silicatização e aplicação do silano na resistência de união das cerâmicas de zircônia e um cimento resinoso. Para isso, 120 blocos cerâmicos sinterizados de zircônia nas dimensões de (5 x 5 x 5 mm) foram obtidos, lixados com lixas de granulação decrescente (#600, #800, #1000 e #1200) e divididos aleatoriamente em 12 grupos (n = 10), de acordo com os fatores “tratamento de superfície” (ScSi - silicatização + silanização; ScSBU - silicatização + Single Bond; SBU - SBU sem fotoativação e SBUp - SBU com fotoativação) e “cerâmica” (Lava/3M ESPE, Ceramill Zircônia/ Amann Girrbach e Zirkonzahn/Zirkonzahn). Posteriormente, cilindros de cimento resinoso dual (RelyX Ultimate/3M ESPE) foram confeccionados no centro de cada bloco com auxílio de uma matriz de silicone (Ø=2 mm; h=5 mm) e fotopolimerizados por 40 s (1200 mW/ cm²). Em seguida, as amostras foram armazenadas durante trinta dias em água destilada (37 °C) e submetidas ao ensaio de resistência de união ao cisalhamento (1 mm/min, 100 kgF). Os dados (MPa) foram analisados sob ANOVA (2 fatores) e teste de Tukey (5%). Análises complementares também foram realizadas. ANOVA revelou que apenas o fator “tratamento de superfície” foi significativo (p=0,0001). O tratamento ScSi (14.28A) promoveu valores de adesão estatisticamente superiores aos demais ScSBU (9.03B), SBU (8.47B) e SBUp (7.82B), os quais foram semelhantes entre si (Tukey). A Análise de falhas revelou que 100% da falhas que ocorreram foram mistas. A silicatização seguida da silanização promoveu a melhor resistência de união entre cimento resinoso e a cerâmica, independentemente do tipo da cerâmica ou do SBU.

Abstract This study evaluated the effectiveness of a multi-mode adhesive (SBU-Scotch Bond Universal/3M) as a substitute for silica coating and silane application on the bonding of zirconia ceramics to resin cement. One-hundred and twenty sintered zirconia ceramic blocks (5 x 5 x 5 mm) were obtained, finished by grounding with silicon carbide paper (#600, #800, #1000 and #1200) and randomly divided into 12 groups (n=10) in accordance with the factors “surface treatment” (ScSi - silicatization + silanization; ScSBU - silicatization + SBU; SBU - SBU without photoactivation and SBUp - SBU photoactivated) and “ceramic” (Lava / 3M ESPE, Ceramill Zirconia / Amann Girrbach and Zirkonzahn / Zirkonzahn). Dual resin cement cylinders (RelyX Ultimate/3M ESPE) were subsequently produced in the center of each block using a silicon matrix (Ø=2 mm, h=5 mm) and photoactivated for 40 s (1200 mW/cm2). The samples were stored for 30 days in distilled water (37ºC) and submitted to shear bond strength test (1 mm/min, 100 KgF). Data (MPa) were analyzed under ANOVA (2 levels) and Tukey test (5%). Complementary analyzes were also performed. ANOVA revealed that only the factor “surface treatment” was significant (p=0.0001). The ScSi treatment (14.28A) promoted statistically higher bond strength values than the other ScSBU (9.03B), SBU (8.47B) and SBUp (7.82B), which were similar to each other (Tukey). Failure analysis revealed that 100% of the failures were mixed. The silica coating followed by the silanization promoted higher bond strength values of resin cement and ceramic, regardless of the zirconia ceramic or SBU.

RESUMO Objetivou-se avaliar o efeito de dois teores de proteína bruta no suplemento mineralnitrogenado para terminação de novilhos Nelore. Foram utilizados 90 animais, com peso médio inicial de 382,35±32,95 kg, submetidos a dois tratamentos: suplemento com 30% de proteína bruta (75% de milho, 8,91% ureia e 16,09% mineral) e 40% de proteína bruta (66,67% de milho, 11,90 % ureia e 21,43% mineral). Os animais foram mantidos em dois piquetes de Uruchloa brizantha cv. MG-5 (sin. Brachiaria brizantha). Foram realizadas pesagens ao início, aos 45 e aos 94 dias de experimento, quando foram abatidos com 451,1±35,38 kg. Foi avaliado o consumo de suplemento, o ganho de peso e variáveis econômicas. Houve efeito do tratamento sobre o consumo de suplemento (P<0,05), com médias de 0,69±0,21 (40% PB) e 2,26±0,52 (30% PB) kg/dia. A média de ganho de peso total foi superior (P<0,01) para os animais que receberam suplemento com 30 % PB (71,46 vs 64,33 kg/animal). Foi obtido maior peso de abate nos animais do tratamento com 30% PB (456,66 vs. 443,84 kg). Em ambos tratamentos, o desempenho e as caracteristicas de carcaça foram satisfatórios. Sob o ponto de vista econômico, recomenda-se a suplementação com 40 % PB na terminação de novilhos em pastagens, porém o sucesso para redução dos custos de produção no campo depende das variações entre as cotações nas diferentes regiões do país e mercado de oportunidades.

SUMMARY The objective of this study was to evaluate the effects of two protein levels in mineral-protein-energy supplements on the productivity and economic performance of Nellore steers. Ninety animals weighing 382.35±32.95 kg on average, were treated with mineral-nitrogen supplements containing 30% crude proteins (CP; 75% ground maize, 8.91 % urea and 16.09% mineral) and 40% CP (66.67 % ground maize, 11.90% urea, and 21.43% mineral). The animals were housed in two paddocks of Uruchloa brizantha ‘MG-5’ (syn. Brachiaria brizantha) and were weighed at the beginning, at 45 days, and at 94 days of the experiment, when they were slaughtered at an average weight of 451±35.38 kg. We evaluated supplement intake, weight gain and economic aspects. The 40 and 30% CP treatments significantly affected supplement intake (P<0.05), with averages of 0.69 and 2.26 kg/day, respectively. The average total weight gain was higher (P<0.01) in animals receiving the supplement containing 30 % CP (71.46 kg / animal) than in animals receiving 40% CP (64.33 kg/animal). Greater slaughter weight was attained in the animals treated with 30% CP (456.66 vs. 443.84 kg). The mineral-nitrogen supplementation in both CP levels provided satisfactory animal performance and carcass characteristics. Owing to economic considerations, it is recommended to use 40% CP mineral-nitrogen supplements on finishing steers on pasture, although success in reducing production costs of livestock ultimately depends on the variations in prices between the different regions of the country and market opportunities.

RESUMO Podisus nigrispinus (Dallas) (Hemiptera: Pentatomidae) são percevejos predadores utilizados como agentes de controle biológico de lagartas desfolhadoras em florestas de eucalipto. Criações massais de inimigos naturais produzem grande número de insetos, mas o número de predadores produzidos pode não coincidir com a época de surtos populacionais de pragas no campo. O objetivo deste trabalho foi avaliar o efeito de diferentes períodos de armazenamento em ovos de P. nigrispinus em baixas temperaturas (5 ºC, 13 ºC e 15 ºC). Os tratamentos consistiram dos períodos de armazenamento (cinco, 10, 15 e 20 dias) dos ovos do predador nas temperaturas indicadas. Após os períodos de armazenagem, os ovos foram retirados e avaliados os parâmetros: viabilidade dos ovos, sobrevivência e duração do estádio ninfal e razão sexual dos adultos emergidos. Os períodos de armazenamento de ovos a 5ºC reduziram a porcentagem de ninfas eclodidas inviabilizando a maioria dos ovos. Enquanto valores de viabilidade próximos a 50% foram observados para os períodos de armazenamento de até 10 dias para 13 ºC e 15 dias armazenados a 15 ºC, com taxas de sobrevivência de ninfas, similares às do controle. Não houve efeito na duração do estágio ninfal e na razão sexual de P. nigrispinus nos tratamentos em cada uma das temperaturas. Portanto, conclui-se que a temperatura de armazenamento de 5 ºC é altamente prejudicial e inviabilizou a maioria dos embriões, enquanto a 13 ºC e 15 ºC é possível armazenar ovos de P. nigrispinus por até 10 e 15 dias, respectivamente.

ABSTRACT Podisus nigrispinus are bedbug predators that are used as biological control agents of defoliating larvae in eucalypt plantations. The mass rearing of natural enemies produces large numbers of insects, but the number of predators produced may not coincide with the timing of the outbreaks of pest populations in the field. The aim of this study was to evaluate the effects of different storage periods on the eggs of P. nigrispinus at low temperatures of 5, 13 and 15ºC. The treatments consisted of periods of 5, 10, 15 and 20 days of storage of predator eggs at each temperature indicated. After the different periods of storage, the eggs were removed, and the egg viability, the survival and duration of nymphal instars and the sex ratio of emerged adults were evaluated. Egg storage at 5ºC reduced the percentage of hatched eggs, while viability values close to 50% were observed for storage periods of up to 10 days at 13ºC and up to 15 days at 15ºC. For these treatments, the survival rates of nymphs were similar to those of the control. The different storage periods had no effect on the duration of the nymphal stage or the sex ratio of P. nigrispinus at any of the temperatures. Therefore, the storage temperature of 5ºC caused the death of the majority of the embryos, while it is possible to store P. nigrispinus eggs up to 10 and 15 days at 13 and 15ºC, respectively.

ABSTRACT Introduction: There is a strong commitment in pursuing reconstructive solutions for areas of skin or muscular loss that require covering or stable cavity filling. The anterolateral thigh flap, described in China by Song et al. (1984), is considered by many as the optimal flap in large reconstructions. Objective: To report the applicability of the anterolateral thigh flap, as a versatile resource in reconstructions of the abdominal wall. Methods: Two patients were submitted to tumoral resection of the right colon with invasion of the abdominal wall by a team of oncologic surgeons in conjunction with the plastic surgery team that was responsible for the reconstruction of the abdominal wall. In both cases, the pedicled anterolateral thigh flap was used, which was transposed to the defect after partial resection of the abdominal wall at the same surgical time. A PROCEEDTM surgical mesh was fixed between the abdominal viscera and the flap to reinforce the abdominal wall. Results: Both patients had good postoperative evolution and are in follow-up, with no signs of tumor recurrence and with a good quality of life. Conclusion: The anterolateral thigh flap is a useful resource for the reconstruction of the abdominal wall due to large tumor resections in oncology.

RESUMO Introdução: Há um grande empenho na busca por soluções reconstrutivas para as áreas de perda cutânea ou muscular que exijam cobertura ou preenchimento cavitário estável. O retalho anterolateral da coxa, descrito na China por Song et al. (1984), é considerado por muitos como o retalho ideal nas grandes reconstruções. Objetivo: Relatar a aplicabilidade do retalho anterolateral da coxa como recurso versátil nas reconstruções da parede abdominal. Métodos: Dois pacientes foram selecionados para serem submetidos à ressecção tumoral de cólon direito com invasão da parede abdominal pela equipe de cirurgia oncológica em conjunto com a equipe de cirurgia plástica, que foi a responsável pela reconstrução da parede abdominal. Em ambos os casos, utilizou-se o retalho anterolateral da coxa pediculado, que foi transposto para o defeito após a ressecção parcial da parede abdominal no mesmo tempo cirúrgico. Entre as vísceras abdominais e o retalho, foi fixada tela de PROCEEDTM para reforço da parede abdominal. Resultados: Ambos os pacientes tiveram boa evolução pós-operatória e encontram-se em acompanhamento, sem sinais de recidiva tumoral e com boa qualidade de vida. Conclusão: O retalho anterolateral da coxa mostrou-se recurso útil dentro do arsenal terapêutico reconstrutivo da parede abdominal devido a grandes ressecções tumorais em oncologia.

Metastatic lesions to the mandible may be originated from primary tumors elsewhere in the body. However, metastatic colonic carcinomas to this bone have been described infrequently. We report the case of a 71-year-old man with an adenocarcinoma in the sigmoid colon with liver metastasis. The patient underwent chemotherapy with indication of sigmoidectomy and retroperitoneal lymphadenectomy. One year and four months after the first metastatic diagnosis, the patient presented a tumor mass in the body and branch of the right mandible. Histopathological and immunohistochemical analysis with monoclonal antibodies specific for CEA, CK20, CDX-2, and vilin were compatible with the diagnosis of moderately differentiated metastatic adenocarcinoma with colonic origin. However, due to the wide spread of the disease, the patient died four months later. Tumor markers have been applied in clinical practice to assist in the diagnosis and to help guide prognosis, staging and treatment of cancer. The management of metastatic lesions remains a controversial issue and the development of new and more specific markers of gastrointestinal differentiation that may promote early diagnosis, are of continuous interest.

Objetivo: Analisar variações anatômicas relacionadas ao bloqueio do plexo celíaco por meio da simulação por tomografia computadorizada e avaliar a possibilidade de transfixação de órgãos pelo método transcrural. Materiais e Métodos: Cento e oito imagens de tomografias computadorizadas transaxiais abdominais foram analisadas. As relações aorto-vertebral, tronco celíaco (TCe)-vertebral, TCe-aórtica e celíaco-aorto-vertebral foram registradas. Em cada imagem foram dispostas duas trajetórias de agulhas, a 9 cm e a 4,5 cm à esquerda e à direita da linha média. Os órgãos vitais transfixados e associações relacionadas ao gênero foram registrados. Resultados: Aorto-vertebral - 45,37% esquerda e 54,62% central; TCe-vertebral - T12, 36,11%; T12-L1, 32,4%; L1, 27,77%; T11-T12, 2,77%; TCe-aórtica - 53,7% esquerda e 46,3% central; celíaco-aorto-vertebral - L-l, 22,22%; M-m, 23,15%; L-m, 31,48%; M-l, 23,15%. Em nenhum dos critérios analisados houve correspondência no lado direito e nem associação significativa entre os gêneros. Conclusão: O bloqueio do plexo celíaco não deve ser padronizado, em razão das amplas variações anatômicas abdominais e das características próprias de cada acesso, sendo necessário o registro de imagem prévio ao procedimento para cada paciente, visando diminuir riscos de lesão. Registros sobre a variação anatômica quanto ao gênero, relacionados ao bloqueio do plexo celíaco, devem ser aprofundados.

Objective: To analyze anatomical variations associated with celiac plexus complex by means of computed tomography simulation, assessing the risk for organ injury as the transcrural technique is utilized. Materials and Methods: One hundred eight transaxial computed tomography images of abdomen were analyzed. The aortic-vertebral, celiac trunk (CeT)-vertebral, CeT-aortic and celiac-aortic-vertebral topographical relationships were recorded. Two needle insertion pathways were drawn on each of the images, at right and left, 9 cm and 4.5 cm away from the midline. Transfixed vital organs and gender-related associations were recorded. Results: Aortic-vertebral - 45.37% at left and 54.62% in the middle; CeT-vertebral - T12, 36.11%; T12-L1, 32.4%; L1, 27.77%; T11-T12, 2.77%; CeT-aortic - 53.7% at left and 46.3% in the middle; celiac-aortic-vertebral - L-l, 22.22%; M-m, 23.15%; L-m, 31.48%; M-l, 23.15%. Neither correspondence on the right side nor significant gender-related associations were observed. Conclusion: Considering the wide range of abdominal anatomical variations and the characteristics of needle insertion pathways, celiac plexus block should not be standardized. Imaging should be performed prior to the procedure in order to reduce the risks for injuries or for negative outcomes to patients. Gender-related anatomical variations involved in celiac plexus block should be more deeply investigated, since few studies have addressed the subject.

Enterococcus faecalis é uma espécie bacteriana frequentemente encontrada em canais radiculares com insucesso do tratamento endodôntico. Pastes alcalinas são amplamente utilizada em Endodontia por causa de sua biocompatibilidade e atividade antimicrobiana, porém esse microrganismo pode ser resistente a alcalinidade. Este estudo avaliou in vitro a resistência do E. faecalis ao pH alcalino por diferentes períodos até 14 dias. Amostras foram obtidas da cavidade oral de 150 pacientes da Clínica de Endodontia. O pH dos tubes experimentais (n=84) foram inicialmente ajustados com NaOH 6M a valores de pH 9.5, 10.5, 11.5 e 12.5 (21 tubes per pH). Vinte isolados clínicos e a cepa ATCC 29212 foram testados. Os 5 controles positivos e os tubos experimentais de cada pH foram inoculados com 10 µL de suspensão bacteriana e incubados a 36 °C por 24, 48 e 72 h, 7 e 14 dias. Para cada período, turvação do meio foi compara visualmente com padrão 0.5 da escala de McFarland. A presença de microorganism foi confirmada por semeadura no meio ágar M-Enterococcus. Quatro tubos contendo caldo BHI ajustado aos pHs testados foram incubados por 14 dias para verificar a ocorrência de alterações de pH. O pH do caldo BHI inoculado também foi medido no 14° dia para determinar se o microrganismo acidificou o meio. O crescimento de todas as cepas de E. faecalis ocorreu com pH entre 9.5 e 11.5 em todos os períodos. Embora não tenha sido observada turvação do meio no pH 12.5, houve crescimento de 13 e 2 cepas às 24 e 48 h, respectivamente, no meio ágar M-Enterococcus. Nenhum tube apresentou crescimento bacteriano no pH 12.5 após 72 h. Concluiu-se que o E. faecalis pode sobreviver em pH altamente alcalino, que alguns isolados clínicos requerem 72 h em pH 12.5 para serem eliminados.

Enterococcus faecalis is a bacterial species often found in root canals with failed endodontic treatment. Alkaline pastes are widely used in Endodontics because of their biocompatibility and antimicrobial activity, but this microorganism can resist alkalinity. The purpose of this study was to evaluate in vitro the alkaline pH resistance of E. faecalis for different periods up to 14 days. Samples were obtained from the oral cavity of 150 patients from the Endodontic clinic. The pH of the experimental tubes (n=84) was first adjusted with 6M NaOH to pH values of 9.5, 10.5, 11.5 and 12.5 (21 tubes per pH). Twenty clinical isolates and the ATCC 29212 strain were tested. The 5 positive controls and experimental tubes of each pH were inoculated with 10 µL of bacterial suspension and incubated at 36 °C for 24, 48 and 72 h, 7 and 14 days. For each period, the turbidity of the medium was visually compared with a 0.5 McFarland standard. The presence of the microorganism was confirmed by seeding on M-Enterococcus agar. Four tubes containing BHI broth adjusted to the tested pHs were incubated for 14 days to verify if pH changes occurred. The pH of inoculated BHI broth was also measured on day 14 to determine if the microorganism acidified the medium. The growth of all E. faecalis strains occurred at pH 9.5 to 11.5 in all periods. Although turbidity was not observed at pH 12.5, there was growth of 13 and 2 strains at 24 and 48 h, respectively, on M-Enterococcus agar. No tube showed growth at pH 12.5 after 72 h. It was concluded that E. faecalis can survive in highly alkaline pH, and some clinical isolates require 72 h at pH 12.5 to be killed.

O "mulching" tem assumido importante papel na cobertura dos solos, no entanto existem alguns procedimentos técnicos a serem ajustados para as novas condições modificadas para o estabelecimento da necessidade total adequada de água à cultura. É necessário, ainda, observar os processos de distribuição da água no solo e a conformação do bulbo molhado que justifiquem a utilização de tais técnicas. Por conseguinte, este trabalho teve por objetivo quantificar o consumo real de água pela cultura do melão rendilhado em ambiente protegido sob "mulching" e verificar a distribuição espacial de água ao redor do sistema radicular, em duas classes de solo, franco-arenoso e argiloso. O "mulching" permitiu a economia de 27 mm de água ao reduzir a evaporação de água. Em termos de volume, cada planta recebeu, em média, a quantia de 175,2 L de água nos 84 dias de cultivo, enquanto nos tratamentos com "mulching", a necessidade hídrica foi de 160,2 L de água por planta. A utilização do "mulching" favoreceu a redução na variabilidade da umidade ao longo do ciclo da cultura e permitiu maior distribuição de água no solo, com maior intensidade no argissolo. O Argissolo proporcionou a retenção de 43 mm a mais que o Latossolo na camada que abrange até 50 m de profundidade e reduziu 5,6 mm a variação de umidade do solo durante o ciclo da cultura, comparado ao Latossolo.

Mulching has become an important technique for land cover, but there are some technical procedures which should be adjusted for these new modified conditions to establish optimum total water depth. It is also important to observe the soil-water relations as soil water distribution and wetted volume dimensions. The objective of the present study was to estimate melon evapotranspiration under mulching in a protected environment and to verify the water spatial distribution around the melon root system in two soil classes. Mulching provided 27 mm water saving by reducing water evaporation. In terms of volume each plant received, on average, the amount of 175.2 L of water in 84 days of cultivation without mulching, while when was used mulching the water requirement was 160.2 L per plant. The use of mulching reduced the soil moisture variability throughout the crop cycle and allowed a greater distribution of soil water that was more intense in the clay soil. The clayey soil provided on average 43 mm more water depth retention in 0.50 m soil deep relative to the sandy loam soil, and reduced 5.6 mm the crop cycle soil moisture variation compared to sandy loam soil.

The objective of this study was to identify the pattern of deposition of feces by supplemented crossbred dairy cows after the lactation peak on cultivated areas under levels of intensification of the system and its effects on nitrogen cycling, with the aid of geostatistics, in the rainy period. Treatments were composed of two levels of nitrogen fertilization (equivalent to 400 and 800 kg N/ha/year) and two types of animals, according to the daily milk production. Geostatistical and descriptive analyses of the data were conducted. The data presented positive coefficients of skewness and platykurtic kurtosis. For the coefficient of variation, there was elevated alteration, with rest areas showing higher values and paddocks with a higher level of fertilization showing lower values.The climate factors radiation, temperature and relative humidity significantly influenced the dispersion and location of feces. As to the degree of spatial dependence, the classification varied from moderated to strong. The range was from 14.0 to 12.7 m for the rest areas and paddocks, respectively. Fertilization and concentrate supply affected the deposition and loss of nitrogen via feces, elevating its values as the nutritional uptake is elevated. Fecal deposition showed heterogeneity, with areas of greater concentration such as shades, entrance of the paddocks and threshing floor, showing deposition peaks that reach 1,051.2 kg N/ha/year.