Pesquisa | SciELO

Resumo Os microRNAs (miRNAs) são reconhecidos como biomarcadores do diabetes mellitus tipo 2 (DM2), úteis para a compreensão do metabolismo da doença, e possuem grande potencial como alvos terapêuticos. O aumento da expressão de BDNF e IGF1 está altamente envolvido nos benefícios as vias de insulina e glicose, porém, são regulados negativamente em condições de resistência à insulina, enquanto seu aumento de expressão está correlacionado com a melhora do metabolismo da glicose e da insulina. Estudos sugerem a regulação desses genes por microRNA em vários contextos diferentes, proporcionando uma nova abordagem de investigação para compreender o metabolismo do DM2 e revelar potenciais alvos terapêuticos. No presente estudo, investigamos em diferentes modelos animais (humanos, ratos e camundongos) miRNAs que têm como alvo BDNF e IGF1 em tecido muscular esquelético com condições fisiológicas de DM2. As análises foram realizadas utilizando ferramentas de bioinformática e bancos de dados para predição de miRNA, homologia molecular, validação experimental de interações, expressão na condição fisiológica estudada e interação em rede. Os resultados mostraram três candidatos a miRNAs para IGF1 (miR-29a, miR-29b e miR-29c) e um para BDNF (miR-206). As avaliações experimentais e a busca pela expressão no músculo esquelético de indivíduos com DM2 confirmaram a interação prevista entre miRNA-mRNA para miR-29b e miR-206 através de modelos humanos, ratos e camundongos. Essa interação foi reafirmada em múltiplas análises de rede. Em conclusão, nossos resultados mostram a relação de regulação entre miR-29b e miR-206 com os genes investigados, em diversos tecidos, sugerindo um padrão de inibição. Contudo, esses dados mostram um grande número de possíveis processos fisiológicos de interação para perspectivas biotecnológicas.

Abstract microRNAs (miRNAs) are recognized as diabetes mellitus type 2 (T2DM) biomarkers useful for disease metabolism comprehension and have great potential as therapeutics targets. BDNF and IGF1 increased expression are highly involved in the benefits of insulin and glucose paths, however, they are down-regulated in insulin resistance conditions, while their expression increase is correlated to the improvement of glucose and insulin metabolism. Studies suggest the microRNA regulation of these genes in several different contexts, providing a novel investigation approach for comprehending T2DM metabolism and revealing potential therapeutic targets. In the present study, we investigate in different animal models (human, rat, and mouse) miRNAs that target BDNF and IGF1 in skeletal muscle tissue with T2DM physiological conditions. Bioinformatics tools and databases were used to miRNA prediction, molecular homology, experimental validation of interactions, expression in the studied physiological condition, and network interaction. The findings showed three miRNAs candidates for IGF1(miR-29a, miR-29b, and miR-29c) and one for BDNF (miR-206). The experimental evaluations and the search for the expression in skeletal muscle from T2DM subjects confirmed the predicted interaction between miRNA-mRNA for miR-29b and miR-206 through human, rat, and mouse models. This interaction was reaffirmed in multiple network analyses. In conclusion, our results show the regulation relationship between miR-29b and miR-206 with the investigated genes, in several tissues, suggesting an inhibition pattern. Nevertheless, these data show a large number of possible interaction physiological processes, for future biotechnological prospects.

https://doi.org/10.1590/1519-6984.256691

Analysis of Residues of Pesticides in Tomato Processed Foods
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Costa, Fátima R. S.

; Maia, Priscilla L.

; Silva, Felipe S. da

; Nobre, Crisiana A.

; Silva, Renata O.

; Milhome, Maria A. L.

Journal of the Brazilian Chemical Society

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Nov 2023, Volume 34 Nº 11 Páginas 1734 - 1742

Resumo: En | Texto: En | PDF: En

The tomato (Solanum lycopersicon L.) crop has great economic relevance, being one of the most processed agricultural products worldwide. Some pesticides are chemically stable and can remain in food even after processing. In this context, the objective of this work was to analyze food products derived from tomatoes (extract, sauce and ketchup), regarding the levels of pesticide residues. Fifteen samples were acquired from Vale do Jaguaribe, Ceará, Brazil. Six pesticides (bifenthrin, chlorothalonil, chlorpyrifos, cyfluthrin, pyriproxyfen, trifluralin) authorized by Agência Nacional de Vigilância Sanitária (Anvisa) for tomato cultivation were selected for the study. QuEChERS (quick, easy, cheap, effective, rugged, safe) extraction and gas chromatography coupled to mass spectrometry (GC-MS) were used in method validation, according to the recommendations of SANTE 11312/2021 guidelines. Statistical analyses of linearity showed that the six pesticides studied were classified as heterocesdastic. Limits of detection (LOD) and quantification (LOQ) values (0.01-0.03 and 0.03-0.10 mg kg-1, respectively) were below the established maximum residue limits (MRLs) for tomatoes. Accuracy and precision (78-121 and 2.3-16.7%, respectively) were satisfactory. The results indicated that among the 15 analyzed samples, one active ingredient was detected (0.05 mg kg-1) in a tomato sauce sample, lower than MRLs (Anvisa 0.15 mg kg-1 and FAO 0.3 mg kg-1). The results show the relevance of monitoring pesticide residues in tomato-derived products and the validation of new methodologies for food control.

https://doi.org/10.21577/0103-5053.20230140

Dereplication of Sclerotiorin-Like Azaphilones Produced by Penicillium meliponae Using LC-MS/MS Analysis and Molecular Networking
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Peres, Eldrinei G.

; Souza, Mayane P.

; Sousa, Thiago F.

; Silva, Carlos V. A. da

; Barros, André L.

; Silva, Felipe M. A. da

; Costa, Emmanoel V.

; Medeiros, Lívia S. de

; Forim, Moacir R.

; Souza, Afonso D. L. de

; Paz, Weider H. P.

; Silva, Gilvan F. da

; Souza, Antonia Q. L. de

; Koolen, Hector H. F.

Journal of the Brazilian Chemical Society

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Ago 2023, Volume 34 Nº 8 Páginas 1139 - 1153

Resumo: En | Texto: En | PDF: En

Penicillium meliponae, a recently described and rare species, was isolated as an endophytic fungus from the Amazonian plant Duguetia sthelechantha, and has been proven to be a pigment producer. Considering the high productivity of this species and the lack of data on its chemical composition, the present study aimed to characterize the chemical profile of P. meliponae and evaluate the influence of agitation and the use of different culture media. For this purpose, liquid chromatography coupled with mass spectrometry (LC-MS/MS) and molecular networking were used, allowing the identification of 17 azaphilone molecules with sclerotiorin-like skeletons, becoming the first chemical report of this species. In addition, the different production patterns in the tested culture media were indicative that this species is sensitive to changes in the composition of the carbon source and to the presence of agitation. Furthermore, this work contributes to the fragmentation mechanisms of the different possible structural arrangements for azaphilones of the sclerotiorin type and serves as a repository of information on the gas-phase behavior of this type of metabolite in mass spectrometry experiments and will assist future studies aimed at the discovery of azaphilones.

https://doi.org/10.21577/0103-5053.20230027

Micromineral concentrations (copper, cobalt, iron, molybdenum and zinc) in the liver of dairy cows from Campos Gerais Region, Paraná state, Brazil
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Pagno, Kialane C.A.

; Barbosa, José D.

; Salvarani, Felipe M.

; Bomjardim, Henrique A.

; Faial, Kelson C.F.

; Sousa, Renato S.

; Gava, Aldo

; Perotta, João H.

; Barros Filho, Ivan R.

Pesquisa Veterinária Brasileira

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2023, Volume 43 elocation e07137

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

ABSTRACT: This study collected samples from 50 Holstein cows, most intensively bred, and from the Campos Gerais region, Paraná, with an average milk production of 30.21L/day. Samples of the liver, spleen and lymph nodes were collected to determine the levels of copper, cobalt, iron, molybdenum and zinc. Spleen and lymph nodes were subjected to histological analysis and evaluation of the degree of hemosiderosis. The average concentrations of copper (495.05ppm), molybdenum (4.19ppm), and zinc (274.49ppm) were higher than those established for the bovines. For cobalt 26% of the animals presented levels below the established level, which characterized cobalt deficiency. Only iron (299.12ppm) exhibited an adequate average level. Histopathologically hemosiderosis was observed mainly in the spleen (78%) and less frequently in the lymph nodes (20%). The observation of hemosiderin in the spleen and lymph nodes is not related to copper deficiency. Still it may be related to high levels of molybdenum, zinc, iron, or other undetermined causes.

RESUMO: Para este estudo foram coletadas amostras de 50 vacas, da raça Holandesa, a maior parte criada intensivamente e oriunda da região dos Campos Gerais/PR, com média individual de produção de leite de 30,21L/dia. Foram coletadas amostras de fígado, baço e linfonodos para determinação dos níveis de cobre (Cu), cobalto (Co), ferro (Fe), molibdênio (Mo) e zinco (Zn). Baço e linfonodo foram submetidos à análise histológica e avaliação do grau de hemossiderose. A média das concentrações de Cu (495,05ppm), Mo (4,19ppm) e Zn (274,49ppm) encontrava-se acima dos níveis estabelecidos para bovinos. Para o Co, observou-se que 26% dos animais apresentaram níveis abaixo do estabelecido, o que caracterizou deficiência de Co. Apenas o Fe (299,12ppm) apresentou níveis médios adequados. Na histopatologia, hemossiderose foi observada, principalmente no baço (78%) e com menos frequência nos linfonodos (20%). A observação de hemossiderina no baço e linfonodos não está relacionada à deficiência de Cu, porém pode estar relacionada a elevados níveis de Mo, Zn e Fe ou a outras causas não determinadas.

https://doi.org/10.1590/1678-5150-pvb-7137

Herpetofauna of protected areas in the Caatinga VIII: An updated checklist for the Serra das Confusões region with new data from Serra Vermelha, Piauí, Brazil
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Marques, Ricardo

; Garda, Adrian A.

; Furtado, Adriana P.

; Bruinjé, Andre C.

; Protázio, Arielson dos S.

; de Carvalho, Breno F.

; Vieira, Cecília R.

; Gomes, Dandara

; Pantoja, Davi L.

; Figueiredo, Diainara da S.

; Shepard, Donald B.

; Camurugi, Felipe

; Coelho, Felipe E. A.

; Magalhães, Felipe M.

; Caetano, Gabriel H. O.

; Colli, Guarino R.

; Paulino, Henrique M.

; Carvalho, Izabelle T. S.

; Graciene, Joedma

; Alvarenga, Júlio M.

; Clay, Natalie A.

; Albuquerque, Ralph L.

; Bosque, Renan J.

; Faria, Renato

; da Silveira-Filho, Ricardo R.

; Mângia, Sarah

; Cavalcante, Vitor H. G. L.

; Vieira, Washington L. S.

; da Silva, Willianilson P.

; Soares, Yan F. F.

; Mesquita, Daniel O.

Biota Neotropica

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2023, Volume 23 Nº 4 elocation e20231520

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract It is repeatedly stressed the need to characterize the extant biodiversity in tropical ecosystems. However, inventory studies are still progressing slowly in dry ecosystems, leading to the underestimation of their true biodiversity and hindering conservation efforts. In this study, we present primary and secondary data, along with an updated list of amphibians and reptiles from two localities in the São Francisco-Gurguéia region in Piauí. Additionally, we compare the species composition between nine areas within the Caatinga, which were sampled using standardized methods over the past ten years, to examine broader spatial patterns of community composition. To survey reptiles and amphibians, we employed similar methods and sampling efforts in two areas within the Serra das Confusões National Park (SCNP) region. Our surveys recorded a total of 73 species of amphibians and reptiles, of which 24 are new distribution records for the SCNP region. Consequently, our findings increase the known herpetofauna in the region to 94 species. Despite their proximity, the two sites in the SCNP region exhibited only 42% similarity in species composition, and they differed significantly from other areas within the Caatinga. Furthermore, even the closer Caatinga areas presented differences in species composition, highlighting the necessity to evaluate biodiversity across the landscape and contribute to understanding biogeographic patterns.

Resumo É repetidamente enfatizada a necessidade de caracterizar a biodiversidade vivente em ecossistemas tropicais. No entanto, os estudos de inventário ainda estão progredindo lentamente em ecossistemas secos, levando à subestimação de sua verdadeira biodiversidade e dificultando os esforços de conservação. Neste estudo, apresentamos dados primários e secundários, juntamente com uma lista atualizada de anfíbios e répteis de duas localidades na região de São Francisco-Gurguéia, do Piauí. Além disso, comparamos a composição de espécies entre nove áreas dentro da Caatinga, que foram amostradas usando métodos padronizados nos últimos dez anos, para examinar padrões espaciais mais amplos de composição da comunidade. Para estudar répteis e anfíbios, utilizamos métodos e esforços de amostragem semelhantes em duas áreas na região do Parque Nacional da Serra das Confusões (PNSC). Nossos levantamentos registraram um total de 73 espécies de anfíbios e répteis, das quais 24 são novos registros de distribuição para a região do PNSC. Consequentemente, nossos resultados aumentam a herpetofauna conhecida na região para 94 espécies. Apesar da proximidade, os dois locais na região do PNSC exibiram apenas 42% de similaridade na composição de espécies e diferiram significativamente de outras áreas dentro da Caatinga. Mesmo áreas mais próximas da Caatinga apresentaram diferenças na composição de espécies, destacando a necessidade de avaliar a biodiversidade em toda a paisagem e contribuir para a compreensão de padrões biogeográficos.

https://doi.org/10.1590/1676-0611-bn-2023-1520

Accessibility perception of chilean para-athletes, road to santiago 2023
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Contreras Jofré, Nicolás Felipe

; Pinochet Gilli, José Ignacio

; Aguayo Carrasco, Enrique Alejandro

; Binimelis Hermosilla, Matías

; Pérez Nuñez, Sebastián Benjamín

; Vergara Ortúzar, Rodrigo Clemente

; Muñoz Hinrichsen, Fernando

Pensar en Movimiento: Revista de ciencias del ejercicio y la salud

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Dez 2022, Volume 20 Nº 2 Páginas 56 - 74

Resumo: Es Pt En | Texto: Es Pt En | PDF: Es | PDF: Pt | PDF: En

Resumen Contreras-Jofré, N. F., Pinochet-Gilli, J. I., Aguayo-Carrasco, E. A., Binimelis-Hermosilla, M., Pérez-Núñez, S. B., Vergara-Ortúzar, R. C. & Muñoz-Hinrichsen, F. (2022). Percepción de para atletas chilenos, rumbo a Santiago 2023, sobre la accesibilidad. PENSAR EN MOVIMIENTO: Revista de Ciencias del Ejercicio y la Salud, 20(2), 1-18. El objetivo de esta investigación es triple: 1) comprender las percepciones de los paratletas sobre la accesibilidad respecto a las futuras sedes de los Juegos Parapanamericanos Santiago 2023; 2) establecer diferencias en la percepción debidas al origen de la discapacidad y 3) documentar la percepción de paratletas con grandes necesidades de apoyo (AHSN, por sus siglas en inglés). Esta investigación es cuantitativa transversal que, en su primera etapa, desarrolló el instrumento denominado Encuesta de Percepción de Accesibilidad de Para-atletas (PPA-D, por sus siglas en inglés) y, en una segunda etapa, se aplicó a 96 paratletas chilenos (77.8% hombres, edad promedio = 31.0 ± 11.6 años). Para la prueba, se obtuvo un coeficiente de validación = .96. Respecto a las características de la muestra, 16.6% correspondió a participantes con discapacidad visual; un 35.1% era AHSN; el 51.5% estaba vinculado a la alta competencia y el 59.3% se trasladaba por su cuenta a sus entrenamientos. Se identificó una percepción negativa de la accesibilidad, principalmente en cuanto al transporte en microbús, en los elevadores y en los espacios de baños, afectando principalmente a AHSN con discapacidad visual (F1,101 = 14.01, p = .0003). Se concluye que es importante abordar las brechas de género, origen de discapacidad y AHSN para garantizar una participación equitativa bajo un marco de derecho.

Resumo Contreras-Jofré, N. F., Pinochet-Gilli, J. I., Aguayo-Carrasco, E. A., Binimelis-Hermosilla, M., Pérez-Núñez, S. B., Vergara-Ortúzar, R. C. & Muñoz-Hinrichsen, F. (2022). Percepção dos paratletas chilenos, rumo a Santiago 2023, sobre acessibilidade. PENSAR EN MOVIMIENTO: Revista de Ciencias del Ejercicio y la Salud, 20(2), 1-18. O objetivo desta pesquisa é triplo: 1) compreender as percepções dos paratletas sobre acessibilidade com relação às futuras sedes dos Jogos Parapan-Americanos de Santiago 2023; 2) estabelecer diferenças de percepção devido à origem da deficiência portada e 3) documentar a percepção de paratletas com altas necessidades de apoio (AHSN, por suas siglas em inglês). Esta pesquisa é quantitativa transversal que, em sua primeira etapa, desenvolveu o instrumento denominado Enquete de Percepção de Acessibilidade de Paratletas (PPA-D, por suas siglas em inglês) e, em uma segunda etapa, foi aplicada a 96 paratletas chilenos (77,8% homens, idade média = 31,0 ± 11,6 anos). Para o teste, obteve-se um coeficiente de validação = 0,96. Em relação às características da amostra, 16,6% corresponderam a participantes com deficiência visual; 35,1% eram AHSN; 51,5% estavam ligados à alta competência e 59,3% se deslocaram por conta própria para o treinamento. Identificou-se uma percepção negativa de acessibilidade, principalmente com relação ao transporte por micro-ônibus, elevadores e banheiros, afetando principalmente os AHSN com deficiência visual (F1.101 = 14,01, p = 0,0003). Conclui-se que é importante abordar as lacunas de gênero, origem da deficiência e AHSN para garantir uma participação equitativa ao abrigo de um quadro jurídico.

Abstract Contreras-Jofré, N. F., Pinochet-Gilli, J. I., Aguayo-Carrasco, E. A., Binimelis-Hermosilla, M., Pérez-Núñez, S. B., Vergara-Ortúzar, R. C. & Muñoz-Hinrichsen, F. (2022). Accessibility perception of chilean para-athletes, road to Santiago 2023. PENSAR EN MOVIMIENTO: Revista de Ciencias del Ejercicio y la Salud, 20(2), 1-18. The objective of this research is three-fold: 1) to understand Para-athletes' perceptions on accessibility regarding Santiago 2023 Parapan American games' future venues, 2) to establish differences in perception due to the origin of the disability, and 3) document alignment, if any, with athletes with high support needs (AHSN). This research is a two-phased quantitative cross-sectional study, which at the first stage developed the ''Para-athletes Perception on Accessibility Survey” (PPA-D) and, at the second stage, the survey was applied to 96 Chilean Para-athletes (77.8% men, mean age = 31.0 ± 11.6 years old). The test obtained a validation coefficient of = 0.96. Regarding the sample characteristics, 16.6% corresponded to participants with visual impairment, 35.1% were AHSN, 51.5% were linked to high-level competition, and 59.3% travelled on their own to their training sessions. A negative perception of accessibility was found, mainly concerning minibuses, elevators, and restroom spaces, affecting mostly AHSN with visual impairment (F1.101 = 14.01, p = .0003). We conclude that it is important to address gender, disability, and AHSN gaps to guarantee equitable participation under a framework of the law and social rights.

https://doi.org/10.15517/pensarmov.v20i2.49265

Anti-Zika Virus Effects, Placenta Protection and Chemical Composition of Passiflora edulis Seeds Ethanolic Extract
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Xavier, Jadriane A.

; Santos, Jaqueline C.

; Nova, Monique A. Vila

; Gonçalves, Camilla M.

; Borbely, Karen S. C.

; Pires, Keyla S. N.

; Santos, Flávia A. R. dos

; Valentim, Iara B.

; Barbosa, Júnia H. P.

; Silva, Felipe C. da

; Santos, Josué C. C.

; Pinho, Danielle R.

; Lopes, Norberto P.

; Borbely, Alexandre U.

; Goulart, Marília O. F.

Journal of the Brazilian Chemical Society

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Jul 2022, Volume 33 Nº 7 Páginas 701 - 714

Resumo: En | Texto: En | PDF: En

Various beneficial biological activities have been reported for passion fruit (Passiflora edulis), grown in tropical and subtropical regions. However, no study has yet demonstrated its action against the Zika virus (ZIKV) infection. The present study aimed to investigate the anti-ZIKV of the ethanolic passion fruit seed extract (PFSE), from which, liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis allowed to putatively annotate the occurrence of piceatannol, astringin, scirpusin A, scirpusin B, isookanin-7-O-glucoside and naringenin-7-O-glucoside, two quadranguloside derivatives, tyrosine, and phenylalanine. PFSE is well tolerated by cell lines (Vero E6 and HTR-8/SVneo) and tissue explants from the human placenta. With 1- and 24-h treatments, PFSE consistently reduced the viral load and NS1 protein expression of the two strains of ZIKV (MR766 and PE243) in placental explants and cell lines. Thus, PFSE has a promising potential for developing anti-ZIKV treatments, protecting the placenta against infection caused by ZIKV, along with relevant antioxidant potential, represented by ferric reducing antioxidant power (FRAP) of 390.5 Trolox eq per g dry extract and half maximal inhibitory concentration (IC50) for 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) of 12.1 µg mL-1, and more than 90% protection from lipid peroxidation. Although further studies need to be conducted, PFSE treatment was safe and effective in trophoblast cells and placental explants, thus representing a promising class of all-accessible products based on the reuse of industrial by-products.

https://doi.org/10.21577/0103-5053.20220003

2021 clinical practice guideline for the early detection, diagnosis, treatment, and monitoring of patients with axial spondyloarthritis. Colombian Association of Rheumatology
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Bautista-Molano, Wilson

; Saldarriaga-Rivera, Lina M.

; Junca-Ramírez, Alejandro

; Fernández-Aldana, Andrés R.

; Fernández-Ávila, Daniel G.

; Jaimes, Diego A.

; Jauregui, Edwin A.

; Segura-Charry, Juan S.

; Romero-Sanchez, Consuelo

; Felipe-Diaz, Oscar J.

Revista Colombiana de Reumatología

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Jun 2022, Volume 29 Nº 2 Páginas 113 - 124

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

RESUMEN Introducción: La espondiloartritis axial es una enfermedad reumatológica que afecta a individuos jóvenes y tiene una gran repercusión sociolaboral. El retraso en el diagnóstico y el tratamiento se asocia con un mayor deterioro funcional y un impacto negativo en la calidad de vida, por lo que requiere un abordaje multidisciplinario. Objetivo: Desarrollar y formular un conjunto de recomendaciones específicas basadas en la mejor evidencia disponible para la detección temprana, el diagnóstico, el tratamiento y el seguimiento de los pacientes adultos con espondiloartritis axial. Métodos: Se configuró un grupo desarrollador, se formularon preguntas clínicas contestables, se graduaron los desenlaces y se realizó la búsqueda sistemática de la evidencia. El panel de la guía fue multidisciplinario (incluyendo representantes de los pacientes) y balanceado, minimizando el sesgo por conflictos de intereses. Se utilizó la aproximación Grading of Recommendations Assessment, Development and Evaluation (GRADE) para evaluar la calidad de la evidencia, al igual que la dirección y la fortaleza de las recomendaciones. Se presentan 11 recomendaciones relacionadas con diagnóstico (n = 2), tratamiento farmacológico (n = 6), tratamiento no farmacológico (n = 2) y seguimiento (n = 1). Resultados: Se recomienda la radiografía de articulaciones sacroilíacas como primer método diagnóstico, y el uso de escalas de actividad para el seguimiento de los pacientes (ASDAS o BASDAI). Los antiinflamatorios no esteroideos son la primera opción de tratamiento; en caso de intolerancia o dolor residual se recomienda acetaminofén u opioides. En pacientes con compromiso axial se recomienda abstenerse de utilizar medicamentos antirreumáticos modificadores de la enfermedad convencionales ni glucocorticoides sistémicos o locales. En pacientes con falla a los antiinflamatorios no esteroideos, se recomienda un anti-TNFα o un anti-IL17A. En pacientes con falla a anti-TNFα, se recomienda iniciar un anti-IL17A. El ejercicio y la terapia física y ocupacional se recomiendan como parte del tratamiento. Se recomienda no utilizar las terapias no convencionales como única opción de tratamiento. Conclusiones: Este conjunto de recomendaciones proporciona una guía actualizada sobre el diagnóstico y el tratamiento de la espondiloartritis axial.

Abstract Introduction: Axial spondyloarthritis is a rheumatic condition affecting young patients with social and occupational consequences. Diagnosis delay is associated with functional impairment and impact on quality of life, requiring a multidisciplinary approach. Objective: To develop a set of recommendations based on the best available evidence for the early detection, diagnosis, treatment, and monitoring of adult patients with axial spondy-loarthritis. Methods: A working group was established, questions were developed, outcomes were graded, and a systematic search for evidence was conducted. A multidisciplinary panel of members was established (including patient representatives), minimizing bias in relation to conflicts of interest. The GRADE approach "Grading of Recommendations Assessment, Development and Evaluation" was used to assess the quality of the evidence as well as the direction and strength of recommendations. In total, 11 recommendations on diagnosis (n=2), pharmacological treatment (n=6), non-pharmacological treatment (n=2) and monitoring (n=1) are presented. Results: Sacroiliac joint radiography as the first diagnostic method, and the use of disease activity scales for patient monitoring (ASDAS or BASDAI), are recommended. Nonsteroidal anti-inflammatory drugs are the first treatment option; in case of intolerance or residual pain, acetaminophen or opioids are recommended. In patients with axial involvement, it is recommended not to use conventional disease-modifying antirheumatic drugs or systemic or local glucocorticoids. In patients with failure to non-steroidal anti-inflammatory drugs, anti-TNF or anti-IL17A are recommended. In those patients presenting with anti-TNF failure, starting an anti-IL17A is recommended. Exercise, physical and occupational therapy are recommended as part of treatment. It is recommended not to use unconventional therapies as the only treatment option. Conclusions: This set of recommendations provides an updated guideline for the diagnosis, treatment, and monitoring of patients with axial spondyloarthritis.

https://doi.org/10.1016/j.rcreu.2021.08.003

Art and science: impact of semioccluded vocal tract exercises and choral singing on quality of life in subjects with congenital GH deficiency
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Andrade, Bruna M. R. de

; Valença, Eugenia H. O.

; Salvatori, Roberto

; Oliveira Neto, Luiz A.

; Souza, Anita H. O.

; Oliveira, Alaíde H. A.

; Oliveira, Mario C.P.

; Melo, Enaldo V.

; Carvalho, Susana de

; Sales, Neuza J

; Monteiro, Gisane C.

; Lima, José Marcel de

; Annunziato, Marcos Felipe Harder

; Mannis, Guilherme Daniel Breternitz

; Souza, Lucas E. de A.

; Goes, Yasmin D.

; Carvalho, Thayza S.

; Farias, Celiane de

; Santos, Michela P. dos

; Cardoso, Gabriela P. F.

; Sousa, Carla S. Pereira

; Santana, Julia Rodrigues

; Sales, Ester Almeida

; d’Avila, Jeferson Sampaio

; Aguiar-Oliveira, Manuel H.

Archives of Endocrinology and Metabolism

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Abr 2022, Volume 66 Nº 2 Páginas 198 - 205

Resumo: En | Texto: En | PDF: En

ABSTRACT Objectives: Currently, not much is known about the interactions between voice and growth hormone (GH). We have described large kindred with isolated GH deficiency (IGHD) due to a GHRH receptor mutation, resulting in severe short stature and high-pitched voice. These IGHD individuals have little interest in GH treatment, as they consider themselves “short long-lived people”, rather than patients. Interestingly, they report normal general quality of life, but they rate their Voice-Related Quality of Life (V-RQOL) as low. Here, we assessed the social and auditory-perceptual impacts of artistic-intervention voice therapy with semioccluded vocal tract exercises (SOVTE) and choral singing, on their voices. Material and methods: Seventeen GH-naïve adult IGHD individuals were enrolled in a single-arm interventional pre-post study with 13 weekly sessions of choir singing over 90 days. Outcome measures were V-RQOL scores, self-assessment of voice, and auditory-perceptual analysis (GRBAS scale, G: grade of the severity of dysphonia; R: roughness; B: breathiness; A: asthenia; and S: strain). Results: Marked improvements in total (p = 0.0001), physical (p = 0.0002), and socioemotional (p = 0.0001) V-RQOL scores and in self-assessment of voice (p = 0.004) were found. The general grades of vocal deviation (p = 0.0001), roughness (p = 0.0001), breathiness (p = 0.0001) and strain (p = 0.0001) exhibited accentuated reductions. Conclusions: Voice therapy with semioccluded vocal tract exercises and choral training improved social impact and perceptual voice assessments in IGHD subjects and markedly improved their voice-related quality of life. This is particularly important in a setting where GH replacement therapy is not widely accepted.

https://doi.org/10.20945/2359-3997000000449

10.

2021 clinical practice guidelines for the diagnosis, treatment, and follow-up of patients with peripheral spondyloarthritis. Colombian Association of Rheumatology
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Saldarriaga-Rivera, Lina M.

; Bautista-Molano, Wilson

; Junca-Ramírez, Alejandro

; Fernández-Aldana, Andrés R.

; Fernández-Ávila, Daniel G.

; Jaimes, Diego A.

; Jáuregui, Edwin A.

; Segura-Charry, Juan S.

; Romero-Sánchez, Consuelo

; Felipe-Diaz, Oscar J.

Revista Colombiana de Reumatología

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Mar 2022, Volume 29 Nº 1 Páginas 44 - 56

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

ABSTRACT Background: Peripheral spondylarthritis is a chronic Inflammatory disease whose clinical presentation is related to the presence of arthritis, enthesitis and/or dactylitis. This term is used interchangeably with some of its subtypes such as psoriatic arthritis, reactive arthritis, and undifferentiated spondyloarthritis. Objective: To develop and formulate a set of specific recommendations based on the best available evidence for the diagnosis, treatment, and monitoring of adult patients with peripheral spondyloarthritis. Methods: A working group was established, clinical questions were formulated, outcomes were graded, and a systematic search for evidence was conducted. The guideline panel was multidisciplinary (including patient representatives) and balanced. Following the for mal expert consensus method, the GRADE methodology "Grading of Recommendations Assessment, Development and Evaluation" was used to assess the quality of the evidence and generate the recommendations. The clinical practice guideline includes ten recommendations related to monitoring of disease activity (n = 1) and treatment (n = 9). Results: In patients with peripheral spondyloarthritis, the use of methotrexate or sulfasalazine as the first line of treatment is suggested, and local injections of glucocorticoids are conditionally recommended. In patients with failure to cDMARDs, an anti TNFα or an anti IL17A is recommended. In case of failure to bDMARDs, it is suggested to use another bDMARD or JAK inhibitor. In patients with peripheral spondylarthritis associated with inflammatory bowel disease, it is recommended to start treatment with cDMARDs; in the absence of response, the use of an anti TNFα over an anti-IL-17 or an anti-IL-12-23 is recom mended as a second line of treatment. In patients with psoriatic arthritis, the combined use of methotrexate with a bDMARD is conditionally recommended for optimization of dosing. To assess disease activity in Psoriatic Arthritis, the use of DAPSA or MDA is suggested for patient monitoring. Conclusions: This set of recommendations provides an updated guideline on the diagnosis and treatment of peripheral spondyloarthritis.

RESUMEN Antecedentes: La espondiloartritis periférica es una patología Inflamatoria crónica cuya presentación clínica está determinada por la presencia de artritis, entesitis y/o dactilitis. Este término se utiliza indistintamente con algunos de sus subtipos como artritis psoriásica, artritis reactiva y espondiloartritis indiferenciada. Objetivo: Desarrollar y formular un conjunto de recomendaciones específicas basadas en la mejor evidencia disponible para el diagnóstico, el tratamiento y el seguimiento de pacientes adultos con espondiloartritis periférica. Métodos: Se constituyó un grupo desarrollador, se formularon preguntas clínicas, se graduaron los desenlaces y se realizó la búsqueda sistemática de la evidencia. El panel de la guía fue multidisciplinario (incluyendo representantes de los pacientes) y balanceado. Siguiendo el método de consenso formal de expertos, se utilizó la metodología GRADE (Grading of Recommendations Assessment, Development and Evaluation) para para evaluar la calidad de la evidencia y generar las recomendaciones. La guía de práctica clínica incluye 10 recomendaciones: una sobre seguimiento de la actividad de la enfermedad y nueve sobre tratamiento. Resultados: En pacientes con espondiloartritis periférica se sugiere usar metotrexato o sulfasalazina como primera línea de tratamiento y se recomienda en forma condicional la inyección local de glucocorticoides. En los pacientes que fallan a cDMARDs, se recomienda iniciar un anti TNFα o un anti IL17A. Ante falla terapéutica a la primera línea con bDMARDs, se sugiere usar otro bDMARD o un inhibidor JAK. En pacientes con espondiloartritis periférica y enfermedad inflamatoria intestinal asociada, se recomienda iniciar tratamiento con cDMARDs; en ausencia de respuesta, se recomienda el uso de un anti TNFα sobre un anti IL-17 o un anti IL-12-23 como segunda línea de tratamiento. En pacientes con artritis psoriásica se recomienda, de forma condicional, el uso combinado de metotrexato con bDMARD para favorecer la optimización de la dosis de estos. Para evaluar la actividad de la enfermedad en artritis psoriásica, se sugiere el uso del DAPSA o MDA para el seguimiento de los pacientes. Conclusiones: Este conjunto de recomendaciones proporcionan una guía actualizada sobre el diagnóstico y el tratamiento de la espondiloartritis periférica.

https://doi.org/10.1016/j.rcreu.2021.07.005

11.

Evaluation of Flow Accelerated Corrosion in Typical Recovery Boiler Environments of Energy Production Industries
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Moreira, Rogaciano M.

; Almeida, Tatiana C.

; Bandeira, Merlin C. E.

; Assunção, Felipe R. S.

; Carvalho, Juliana F. A.

; Silva, Elaine F.

; Verbicario, João V. T.

; Mattos, Oscar R.

; Oliveira, Jefferson R.

; Bonfim, André L. C.

; Novgorodecev, André R.

Materials Research

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2022, Volume 25 Supl. 1 elocation e20210520

Resumo: En | Texto: En | PDF: En

Abstract Flow accelerated corrosion (FAC) is a steel degradation that occurs in heat recovery steam generators in the power industry. The mechanism of this corrosion comprises an electrochemical dissolution of the semi-protective magnetite layer (Fe3O4) that is formed within the pipes employed in the boilers. The FAC is influenced by different factors such as fluid velocity, pH and dissolved oxygen concentration. In this context, Rotating Cage tests were used to evaluate the influence of pH and dissolved oxygen content on FAC of A210, P11 and P22 steels. General corrosion was lower for P11 and P22 steels, an effect consistent with the presence of chromium in their compositions. General corrosion was most critical at pH 8.5. The corrosion intensity decreased for the three steels when the dissolved oxygen concentration increased, at 2 m/s. This behavior is coherent considering the most effective precipitation of Fe2O3 inside the pores of the Fe3O4 layer in a more oxidizing environment. For 3 m/s, the corrosion intensity increased for both materials when oxygen increased, showing the shear stress effect. These behaviors were more significant at pH 8.5. About pitting corrosion, the analyzes show localized attack in the majority of coupons, however with very low number of pits.

https://doi.org/10.1590/1980-5373-mr-2021-0520

12.

Muscular and cardiorespiratory parameters of Brazilian professional futsal players: comparison between top national and regional level athletes
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David, Gabriela B.

; Alberton, Cristine L.

; Brizio, Maria Laura R.

; Coswig, Victor S.

; Jung, Laura G.

; Silveira, Jennifer R.

; Reichert, Felipe F.

Motriz: Revista de Educação Física

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2022, Volume 28 elocation e10220005921

Resumo: En | Texto: En | PDF: En

Abstract Aim: To compare muscular and cardiorespiratory parameters between Brazilian professional futsal athletes from different competitive levels (national versus regional). A secondary aim was to explore the correlation of physical fitness variables between both competitive levels. Methods: Twenty-two futsal players (24.7 ± 3.7 years), 11 from a regional team and 11 from a national team, were evaluated with countermovement jump test (lower limbs muscle power), square test (the speed with change direction), RSSA (anaerobic power), and YOYOIR1 (aerobic power). Tests were performed in the middle of the season for both teams. Results: The speed with change direction (p = 0.001) and mean anaerobic power (p = 0.04) were higher for national than the regional players. Sprint performance decreased similarly for national and regional level players (p < 0.001), but the latter had poorer performance (p = 0.044). From the fourth sprint on, sprints for the national level players were slower than the first three, while for the regional level players, each sprint speed was slower than the subsequent. A significant correlation was found between anaerobic power with jump height (r = −0.50; p = 0.01), speed with change direction (r=0.65; p = 0.001) and distance covered in the YOYOIR1 (r = −0.54; p = 0.01). Conclusion: National-level athletes were more agile and presented a higher mean anaerobic power than regional ones. Moreover, national-level athletes presented better performance along with repeated sprint tests, maintaining this performance for a longer time during the test.

https://doi.org/10.1590/s1980-657420220005921

13.

Assessing the carbon stock of cultivated pastures in Rondônia, southwestern Brazilian Amazon
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SANQUETTA, CARLOS R.

; BASTOS, ALEXIS DE S.

; SANQUETTA, MATEUS N.I.

; BARBERENA, IARA M.

; CORTE, ANA P. DALLA

; QUEIROZ, ALEXANDRE

; ALMEIDA, LUIZ FELIPE P.U.

Anais da Academia Brasileira de Ciências

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2022, Volume 94 Nº 4 elocation e20210262

Resumo: En | Texto: En | PDF: En

Abstract Cattle ranching is the primary land-use of deforested areas in the Brazilian Amazon. Deforestation precedes pasture establishment, implying tremendous amounts of greenhouse gas emissions caused by carbon stock losses. Despite several studies addressing carbon storage in forests, there is a lack of data regarding cultivated pastures. Hence, the estimation of greenhouse gas emissions associated with land-use change becomes uncertain. In this study, we assessed the carbon stock of cultivated pastures located in Rondônia, southwestern Brazilian Amazon. A total of 50 squared plots of 1 m² were randomly allocated in cattle ranching farms covered by Oxisols (Dystrophic Yellow and Dystrophic Red-Yellow Latosols). Carbon fraction ranged from 0.36 for belowground biomass to 0.45 gC.g-1 d.m. for aboveground biomass. The average total carbon stock was 5.17 MgC.ha-1, with non-significant differences when stratifying data by soil types. Considering data from the III Brazilian Inventory of Anthropogenic Emissions and Removals of Greenhouse Gases, our results suggested that land-use change from primary forests to cultivated pastures resulted in a loss of 192.54 MgC.ha-1, which corresponds to a net emission of 705.98 MgCO2eq.ha-1 to the atmosphere. This study provides valuable information to improve the Brazilian Inventory of Anthropogenic Emissions and Removals of Greenhouse Gases.

https://doi.org/10.1590/0001-3765202220210262

14.

Longitudinal study about the impact of COVID-19 pandemic in a southern Brazilian state: the PAMPA cohort
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FETER, NATAN

; CAPUTO, EDUARDO L.

; DORING, IGOR R.

; LEITE, JAYNE S.

; CASSURIAGA, JÚLIA

; REICHERT, FELIPE F.

; SILVA, MARCELO C. DA

; ROMBALDI, AIRTON J.

Anais da Academia Brasileira de Ciências

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2022, Volume 94 Nº 2 elocation e20201718

Resumo: En | Texto: En | PDF: En

Abstract This study aimed to describe the effects of social distancing due to the COVID-19 pandemic on physical and mental health, chronic disease management, and economic situation in adults from a southern Brazilian state. Data from the baseline of the PAMPA (Prospective Study About Mental and Physical Health) cohort, a population-based, longitudinal study is presented. An online-based questionnaire was used to assess the variables pre- and during social distancing. Most of the respondents (N=2,321) were women (76.7%), white (90.6%), had at least university degree (66.7%), and were overweight or obese (53.3%). Nearly 40% were not able to work from home and 45.3% had monthly income reduced during social distancing. Moderate-to-severe symptoms of depression and anxiety increased 7.3x and 8.4x from before to during social distancing. Three quarters reported low back pain (74.2%; 95%CI: 71.9%, 76.3%) during the same period while the prevalence of physical inactivity increased 31.2%. Managing chronic conditions was harder during social distancing for 28.5% of the participants. Roughly 40% reported that did not seek medical assistance even when necessary during this period. COVID-19 pandemic has disrupted the health of adults from a southern Brazilian state in several aspects.

https://doi.org/10.1590/0001-3765202220201718

15.

MOLECULAR NETWORKING-BASED DEREPLICATION OF AMBUIC ACID DERIVATIVES FROM THE MARINE FUNGUS PESTALOTIOPSIS SP. 4A11
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Kellner Filho, Luis C.

; Assis, Rhenner N. A.

; Santos, Aline O. dos

; Targanski, Sabrina K.

; Siqueira, Kátia A.

; Medeiros, Livia S. de

; Angolini, Célio F. F.

; Silva, Felipe M. A. da

; Soares, Marcos A.

; Dias, Gustavo M.

; Silva, Marcio L. A.

; Cunha, Wilson R.

; Pauletti, Patricia M.

; Koolen, Hector H. F.

; Januario, Ana H.

Química Nova

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2022, Volume 45 Nº 3 Páginas 268 - 274

Resumo: En | Texto: En | PDF: En

Ambuic acid (AA) is a highly-modified cyclohexenone and known as a promising inhibitor of quorum sensing in methicillin-resistant Staphylococcus aureus, and is thus a candidate as an antivirulence drug. This molecule is mainly produced by the species of Pestalotiopsis and, since its discovery twenty years ago, only a restricted amount of AA-derivatives have been described. Despite being a promising subject, methods for the analysis of modified AA-analogues via mass spectrometry remain unexplored. In order to adress this question, the marine fungus Pestalotiopsis sp. 4A11 associated with the ascidian Didemnum perlucidum was grown in a solid rice medium and its crude extract was chemically studied. From this extract, AA and 10-hydroxy ambuic acid (10-HAA) were isolated and identified using NMR spectroscopy with the aim of obtaining model compounds for the MS analysis. These served as reference compounds (seeds) to guide the dereplication of other AA-analogues via LC-MS/MS-based molecular networking. Based on the manual interpretation of the fragmentation pathways of the seeds and related compounds observed in the networks, six AA-derivatives were dereplicated in the extract. Furthermore, three analogues with unprecedented chemical formulas were proposed as putative unprecedented AA-derivatives. The fragmentation annotation proposed represents a fast and feasible method for characterizing AA-derivatives.

https://doi.org/10.21577/0100-4042.20170819

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